SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012169059.1 NCBI__GCF_000010525.1:WP_012169059.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AAI3 ATP-dependent zinc metalloprotease FtsH; Cell division protease FtsH; EC 3.4.24.- from Escherichia coli (strain K12) (see 4 papers)
58% identity, 99% coverage: 6:638/640 of query aligns to 3:624/644 of P0AAI3

query
sites
P0AAI3

R

K

N

N

F

L

A

I

L

L

W

W

V

L

I

V

I

I

V

A

L

V

L

V

L

L

L

M

A

S

L

V

F

F

S

Q

L

S

F

F

Q

-

S

G

P

P

G

S

Q

E

R

-

S

S

S

N

A

G

N

R

D

K

I

V

S

D

F

Y

S

S

Q

T

L

F

L

L

S

Q

D

E

V

V

D

N

Q

N

G

D

R

Q

V

V

R

R

D

E

V

A

V

R

I

I

E

N

G

G

P

R

N

E

I

I

S

N

G

V

S

T

F

K

T

K

D

D

G

S

R

N

Q

R

F

Y

Q

T

T

T

Y

Y

A

I

P

P

-

V

S

Q

D

D

P

P

T

K

L

L

V

L

Q

D

R

N

L

L

Y

L

G

T

K

K

G

N

V

V

S

K

I

V

T

V

A

G

R

E

P

P

P

P

S

E

D

E

N

P

V

-

P

S

W

L

F

L

V

A

S

S

L

I

L

F

V

I

S

S

W

W

L

F

P

P

F

M

I

L

A

L

L

L

I

I

G

G

V

V

W

W

I

I

F

F

L

F

S

M

R

R

Q

Q

M

M

Q

Q

G

G

A

G

G

G

G

G

K

K

-

G

A

A

M

M

G

S

F

F

G

G

K

K

S

S

R

K

A

A

K

R

L

M

L

L

T

T

E

E

A

D

H

Q

G

I

R

K

V

T

T

T

F

F

E

A

D

D

V

V

A

A

G

G

I

C

D

D

E

E

A

A

K

K

S

E

D

E

L

V

T

A

E

E

I

L

V

V

D

E

F

Y

L

L

R

R

D

E

P

P

Q

S

K

R

F

F

Q

Q

R

K

L

L

G

G

G

G

R

K

I

I

P

P

R

K

G

G

V

V

L

L

L

M

V

V

G

G

P

P

P

P

G

G

T

T

G

G

K

K

T

T

L

L

L
|
L

A

A

R

K

A

A

I

I

A

A

G

G

E

E

A

A

N

K

V

V

P

P

F

F

F

F

T

T

I

I

S

S

G

G

S

S

D

D

F

F

V

V

E

E

M

M

F
|
F

V

V

G
|
G

V

V

G

G

A

A

S

S

R

R

V

V

R

R

D

D

M

M

F

F

E

E

Q

Q

A

A

K

K

K

K

N

A

A

A

P

P

C

C

I

I

I

I

F

F

I

I

D

D

E

E

I

I

D

D

A

A

V

V

G

G

R

R

H

Q

R

R

G

G

A

A

G

G

L

L

G

G

G

G

G

G

N

H

D

D

E

E

R

R

E

E

Q

Q

T

T

L

L

N

N

Q

Q

L

M

L

L

V

V

E

E

M

M

D

D

G

G

F

F

E

E

A

G

N

N

E

E

G

G

I

I

I

I

L

V

I

I

A

A

A

A

T
|
T

N
|
N

R

R

P

P

D

D

V

V

L

L

D
|
D

P

P

A

A

L
|
L

L

L

R
|
R

P

P

G

G

R
|
R

F

F

D

D

R

R

Q

Q

V

V

V

V

V

V

P

G

N

L

P

P

D

D

V

V

V

R

G

G

R

R

E

E

Q

Q

I

I

L

L

K

K

V

V

H

H

A

M

R

R

K

R

I

V

P

P

I

L

A

A

P

P

D

D

V

I

N

D

L

A

K

A

V

I

I

I

A

A

R

R

G

G

T

T

P

P

G

G

F

F

S

S

G

G

A

A

D

D

L

L

A

A

N

N

L

L

C

V

N

N

E

E

A

A

A

A

L

L

M

F

A

A

A

A

R

R

R

G

N

N

K

K

R

R

M

V

V

V

T

S

M

M

V

V

E

E

F

F

E

E

D

K

A

A

K

K

D

D

K

K

V

I

M

M

M

M

G

G

A

A

E

E

R

R

R

R

S

S

L

M

V

V

M

M

T

T

E

E

E

A

E

Q

K

K

M

E

L

S

T

T

A

A

Y

Y

H
|
H

E
|
E

G

A

G

G

H
|
H

A

A

I

I

V

I

A

G

L

R

N

L

V

V

P

P

A

E

T

H

D

D

P

P

V

V

H

H

K

K

A

V

T

T

I

I

I

I

P

P

R

R

G

G

R

R

A

A

L

L

G

G

M

V

V

T

M

F

Q

F

L

L

P

P

E

E

R

G

D

D

K

A

L

I

S

S

M

A

S

S

Y

R

E

Q

Q

K

M

L

T
x
E

S

S

R

Q

L

I

A

S

I

T

M

L

M

Y

G

G

G

G

R

R

V

L

A

A

E
|
E

E

E

L

I

I

I

F

Y

G

G

H

P

D

E

K

H

V

V

T

S

S

T

G

G

A

A

A

S

S

N

D

D

I

I

E

K

Q

V

A

A

T

T

R

N

L

L

A

A

K

R

M

N

M

M

V

V

T

T

R

Q

W

W

G

G

F

F

S

S

D

E

E

K

L

L

G

G

Q

P

V

L

A

L

Y

Y

G

A

E

E

N

E

Q

E

D

G

E

E

V

V

F

F

L

L

G

G

M

R

S

S

M

V

G

A

R

K

T

A

Q

K

N
x
H

V

M

S

S

E

D

A

E

T

T

A

A

Q

R

T

I

I

I

D

D

K

Q

E

E

V

V

R

K

R

A

L

L

V

I

D

E

E

R

G

N

Y

Y

V

N

E

R

A

A

K

R

R

Q

I

L

L

L

S

T

E

D

K

N

A

M

V

D

D

I

L

L

E

H

T

A

L

M

A

K

R

D

G

A

L

L

L

M

E

K

Y

Y

E
|
E

T

T

L

I

T

D

G

A

D

P

E

Q

I

I

V

D

D

D

L

L

L

M

N

-

G

-

K

-

P

-

P

-

V

A

R

R

D

R

T

D

V

V

I

R

E

P

P

P

A

A

N

G

P

W

R

E

G

E

P

P

T

-

V

-

P

-

T

-

A

-

G

G

A

A

G

S

K

N

N

N

R

S

P

G

R

D

P

N

G

G

G

S

N

P

P

K

E

A

P

P

Q

R

P

P

L201 (= L205) mutation to N: No in vivo protease activity, no in vitro ATPase activity.
F225 (= F229) mutation F->A,D,E,G,N,Q,R,S,T: Does not complement ftsH1 at 42 degrees Celsius, no protease activity in vivo.; mutation F->C,H: Partially complements ftsH1 at 42 degrees Celsius, some protease activity in vivo.; mutation F->I,L,M,V,W,Y: Complements ftsH1 at 42 degrees Celsius, restores protease activity in vivo.
G227 (= G231) mutation to A: Does not complement ftsH1 at 42 degrees Celsius, no protease activity in vivo.
T297 (= T301) mutation to A: Low protease activity in vivo, low ATPase activity in vitro, complements ftsH1 at 42 degrees Celsius.
N298 (= N302) mutation to A: No in vivo protease activity.
D304 (= D308) mutation D->A,N: No in vivo protease activity, no in vitro ATPase activity; probably still binds ATP.; mutation to E: Low protease activity in vivo, low ATPase activity in vitro, complements ftsH1 at 42 degrees Celsius.
L307 (= L311) mutation to A: Low protease activity in vivo.
R309 (= R313) mutation R->A,L,K: No in vivo protease activity, no ATPase activity in vitro; probably still binds ATP.
R312 (= R316) mutation R->A,L,K: No in vivo protease activity, no ATPase activity in vitro; probably still binds ATP.
H414 (= H418) mutation to Y: Loss of protease function.
E415 (= E419) mutation to Q: Loss of protease activity in vivo.
H418 (= H422) mutation to Y: In tolZ21; loss of protease function in vivo, retains about 25% ATPase activity, temperature sensitive.
E463 (≠ T467) mutation to K: In ftsH1; a temperature-sensitive mutant which increases the frequency of lysogenization of phage lambda; when associated with A-587.
E476 (= E480) mutation E->D,K,V: Severe loss of protease function that is restored by excess Zn.; mutation to Q: Little effect on protease function.
H536 (≠ N540) mutation to R: In hflB29; increases the frequency of lysogenization of phage lambda.
E582 (= E586) mutation E->D,K,Q: No effect on protease function.; mutation to V: Decreased protease function.

Q9WZ49 ATP-dependent zinc metalloprotease FtsH; EC 3.4.24.- from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see 2 papers)
52% identity, 93% coverage: 1:598/640 of query aligns to 1:602/610 of Q9WZ49

query
sites
Q9WZ49

M

M

N

N

-

R

A

S

N

N

L

I

R

W

N

N

-

L

-

L

F

F

A

T

L

I

W

L

V

I

I

I

I

V

V

T

L

L

L

F

L

W

L

L

A

A

-

R

L

F

F

F

S

Y

L

V

F

E

Q

N

S

S

P

P

G

V

Q

S

R

K

S

L

S

S

A

Y

N

T

D

-

I

-

S

S

F

F

S

V

Q

Q

L

M

L

V

S

E

D

D

V

-

D

E

Q

R

G

S

R

V

V

V

R

S

D

E

V

V

V

V

I

I

E

R

G

D

P

D

N

G

I

V

S

L

G

R

S

V

F

Y

T

T

-

K

D

D

G

G

R

R

Q

V

F

Y

Q

E

T

V

Y

D

A

A

P

P

-

W

-

A

-

V

S

N

D

D

P

S

T

Q

L

L

V

I

Q

E

R

K

L

L

Y

V

G

S

K

K

G

G

V

I

S

K

I

V

T

S

A

G

R

E

P

R

P

S

S

G

D

S

N

S

V

S

P

F

W

W

F

-

V

I

S

N

L

V

L

L

V

G

S

T

W

L

L

I

P

P

F

T

I

I

A

L

L

F

I

I

G

V

V

V

W

W

I

L

F

F

L

I

S

M

R

R

Q

S

M

L

Q

S

G

G

A

R

G

N

G

N

K

Q

A

A

M

F

G

T

F

F

G

T

K

K

S

S

R

R

A

A

K

T

L

M

L

Y

T

K

-

P

E

S

A

G

H

N

G

K

R

R

V

V

T

T

F

F

E

K

D

D

V

V

A
x
G

G

G

I

A

D

E

E

E

A

A

K

I

S

E

D

E

L

L

T

K

E

E

I

V

V

V

D

E

F

F

L

L

R

K

D

D

P

P

Q

S

K

K

F

F

Q

N

R

R

L

I

G

G

G

A

R

R

I

M

P

P

R

K

G

G

V

I

L

L

L

L

V

V

G

G

P

P

P

P

G
|
G

T
|
T

G
|
G

K
|
K

T
|
T

L
|
L

L

L

A

A

R

R

A

A

I

V

A

A

G

G

E

E

A

A

N

N

V

V

P

P

F

F

F

F

T

H

I

I

S

S

G

G

S

S

D

D

F

F

V

V

E

E

M

L

F

F

V

V

G

G

V

V

G

G

A

A

S

A

R

R

V

V

R

R

D

D

M

L

F

F

E

A

Q

Q

A

A

K

K

K

A

N

H

A

A

P

P

C

C

I

I

I

V

F

F

I

I

D

D

E

E

I

I

D

D

A

A

V

V

G

G

R

R

H

H

R

R

G

G

A

A

G

G

L

L

G

G

G

G

G

G

N

H

D

D

E

E

R

R

E

E

Q

Q

T

T

L

L

N

N

Q

Q

L

L

L

L

V

V

E

E

M

M

D

D

G

G

F

F

E

D

A

S

N

K

E

E

G

G

I

I

I

I

L

V

I

M

A

A

A

A

T

T

N

N

R

R

P

P

D

D

V

I

L

L

D

D

P

P

A

A

L

L

L

L

R

R

P

P

G

G

R

R

F

F

D

D

R

K

Q

K

V

I

V

V

V

V

P

D

N

P

P

P

D

D

V

M

V

L

G

G

R

R

E

K

Q

K

I

I

L

L

K

E

V

I

H
|
H

A

T

R

R

K

N

I

K

P

P

I

L

A

A

P

E

D

D

V

V

N

N

L

L

K

E

V

I

I

I

A

A

R

K

G

R

T

T

P

P

G

G

F

F

S

V

G

G

A

A

D

D

L

L

A
x
E

N

N

L

L

C

V

N

N

E

E

A

A

A

A

L

L

M

L

A

A

A

A

R

R

R

E

N

G

K

R

R

D

M

K

V

I

T

T

M

M

V

K

E

D

F

F

E

E

D

E

A

A

K

I

D

D

K

R

V

V

M

I

M

A

G
|
G

A

P

E

A

R

R

R

K

S

S

L

K

V

L

M

I

T

S

E

P

E

K

E

E

K

K

M

R

L

I

T

I

A

A

Y

Y

H
|
H

E
|
E

G

A

G

G

H
|
H

A

A

I

V

V

V

A

S

L

T

N

V

V

V

P

P

A

N

T

G

D

E

P

P

V

V

H

H

K

R

A

I

T

S

I

I

I

I

P

P

R

R

G

G

-

Y

R

K

A

A

L

L

G

G

M

Y

V

T

M

L

Q

H

L

L

P

P

E

E

R

E

D

D

K

K

L

Y

S

L

M

V

S

S

Y

R

E

N

Q

E

M

L

T

L

S

D

R

K

L

L

A

T

I

A

M

L

M

L

G

G

G

G

R

R

V

A

A

A

E

E

E

E

L

V

I

V

F

F

G

G

H

D

D

-

K

-

V

V

T

T

S

S

G

G

A

A

A

A

S

N

D
|
D

I

I

E

E

Q

R

A

A

T

T

R

E

L

I

A

A

K

R

M

N

M

M

V

V

T

C

R

Q

W

L

G

G

F

M

S

S

D

E

E

E

L

L

G

G

Q

P

V

L

A

A

Y

W

G

G

E

K

N

E

Q

E

D

Q

E

E

V

V

F

F

L

L

G

G

M

K

S

E

M

I

G

T

R

R

T

L

Q

R

N

N

V

Y

S

S

E

E

A

E

T

V

A

A

Q

S

T

K

I

I

D

D

K

E

E

E

V

V

R

K

R

K

L

I

V

V

D

T

E

N

G

C

Y

Y

V

E

E

R

A

A

K

K

R

E

I

I

L

I

S

R

E

K

K

Y

A

R

V

K

D

Q

L

L

E

D

T

N

L

I

A

V

R

E

G

I

L

L

L

L

E

E

Y

K

E

E

T

T

L

I

T

E

G

G

D

D

E

E

I

L

V

R

D

R

L

I

L

L

N

S

G164 (≠ A159) binding ATP
GTGKT 204:208 (= GTGKT 199:203) binding ATP
L209 (= L204) binding ATP
H343 (= H338) binding ATP
E371 (≠ A366) binding ATP
G404 (= G399) mutation to L: Complete loss of protease activity and of oligomerization.
H423 (= H418) binding Zn(2+)
E424 (= E419) active site
H427 (= H422) binding Zn(2+)
D500 (= D496) binding Zn(2+); mutation to A: Complete loss of protease activity.

P37476 ATP-dependent zinc metalloprotease FtsH; Cell division protease FtsH; EC 3.4.24.- from Bacillus subtilis (strain 168) (see paper)
51% identity, 95% coverage: 1:605/640 of query aligns to 1:610/637 of P37476

query
sites
P37476

M

M

N

N

A

R

N

V

L

F

R

R

N

N

F

T

A

I

L

F

W

Y

V

L

I

L

I

I

V

L

L

L

L

V

L

V

L

I

A

G

L

V

F

V

S

S

L

Y

F

F

Q

Q

S

T

P

S

G

N

Q

P

R

K

S

T

S

E

A

-

N

-

D

N

I

M

S

S

F

Y

S

S

Q

T

L

F

L

I

S

K

D

N

V

L

D

D

Q

D

G

G

R

K

V

V

R

D

D

S

V

V

V

S

I

V

E

Q

G

P

P

-

N

-

I

V

S

R

G

G

S

V

F

Y

T

E

D

V

G

K

R

G

Q

Q

F

L

Q

K

T

N

Y

Y

A

D

P

K

S

D

D

Q

P

Y

T

F

L

L

V

T

Q

H

R

V

L

P

Y

E

G

G

K

K

G

G

-

A

-

D

-

Q

-

I

-

F

-

N

-

A

-

L

-

K

-

K

V

T

S

D

I

V

T

K

A

V

R

E

P

P

P

A

S

Q

D

E

N

T

V

S

P

G

W

W

F

-

V

V

S

T

L

F

L

L

V

T

S

T

W

I

L

I

P

P

F

F

I

V

A

I

L

I

I

F

G

I

V

L

W

F

I

F

F

F

L

L

S

L

R

N

Q

Q

M

A

Q

Q

G

G

A

G

G

G

G

S

K

R

A

V

M

M

G

N

F

F

G

G

K

K

S

S

R

K

A

A

K

K

L

L

L

Y

T

T

E

E

A

E

H

K

G

K

R

R

V

V

T

K

F

F

E

K

D

D

V

V

A

A

G

G

I

A

D

D

E

E

A

E

K

K

S

Q

D

E

L

L

T

V

E

E

I

V

V

V

D

E

F

F

L

L

R

K

D

D

P

P

Q

R

K

K

F

F

Q

A

R

E

L

L

G

G

G

A

R

R

I

I

P

P

R

K

G

G

V

V

L

L

L

L

V

V

G

G

P

P

P

P

G

G

T

T

G

G

K
|
K

T

T

L

L

L

L

A

A

R

K

A

A

I

C

A

A

G

G

E

E

A

A

N

G

V

V

P

P

F

F

F

F

T

S

I

I

S

S

G

G

S

S

D

D

F

F

V

V

E

E

M

M

F

F

V

V

G

G

V

V

G

G

A

A

S

S

R

R

V

V

R

R

D

D

M

L

F

F

E

E

Q

N

A

A

K

K

K

K

N

N

A

A

P

P

C

C

I

L

I

I

F

F

I

I

D

D

E

E

I

I

D

D

A

A

V

V

G

G

R

R

H

Q

R

R

G

G

A

A

G

G

L

L

G

G

G

G

G

G

N

H

D

D

E

E

R

R

E

E

Q

Q

T

T

L

L

N

N

Q

Q

L

L

L

L

V

V

E

E

M

M

D

D

G

G

F

F

E

S

A

A

N

N

E

E

G

G

I

I

I

I

L

I

I

I

A

A

A

A

T

T

N

N

R

R

P

A

D

D

V

I

L

L

D

D

P

P

A

A

L

L

L

L

R

R

P

P

G

G

R

R

F

F

D

D

R

R

Q

Q

V

I

V

T

V

V

P

D

N

R

P

P

D

D

V

V

V

I

G

G

R

R

E

E

Q

A

I

V

L

L

K

K

V

V

H

H

A

A

R

R

K

N

I

K

P

P

I

L

A

D

P

E

D

T

V

V

N

N

L

L

K

K

V

S

I

I

A

A

R

M

G

R

T

T

P

P

G

G

F

F

S

S

G

G

A

A

D

D

L

L

A

E

N

N

L

L

C

L

N

N

E

E

A

A

A

A

L

L

M

V

A

A

A

A

R

R

R

Q

N

N

K

K

R

K

M

K

V

I

T

D

M

A

V

R

E

D

F

I

E

D

D

E

A

A

K

T

D

D

K

R

V

V

M

I

M

A

G

G

A

P

E

A

R

K

R

K

S

S

L

R

V

V

M

I

T

S

E

K

E

K

E

E

K

R

M

N

L

I

T

V

A

A

Y

Y

H

H

E
|
E

G

G

G

G

H

H

A

T

I

V

V

I

A

G

L

L

N

V

V

L

P

D

A

E

T

A

D

D

P

M

V

V

H

H

K

K

A

V

T

T

I

I

I

V

P

P

R

R

G

G

R

Q

A

A

L

G

G

G

M

Y

V

A

M

V

Q

M

L

L

P

P

E

R

R

E

D

D

K

R

L

Y

S

F

M

Q

S

T

Y

K

E

P

Q

E

M

L

T

L

S

D

R

K

L

I

A

V

I

G

M

L

M

L

G

G

G

G

R

R

V

V

A

A

E

E

E

E

L

I

I

I

F

F

G

G

H

-

D

-

K

E

V

V

T

S

S

T

G

G

A

A

A

H

S

N

D

D

I

F

E

Q

Q

R

A

A

T

T

R

N

L

I

A

A

K

R

M

R

M

M

V

V

T

T

R

E

W

F

G

G

F

M

S

S

D

E

E

K

L

L

G

G

Q

P

V

L

A

Q

Y

F

G

G

E

Q

N

S

Q

Q

-

G

D

G

E

Q

V

V

F

F

L

L

G

G

M

R

S

D

M

F

G

N

R

N

T

E

Q

Q

N

N

V

Y

S

S

E

D

A

Q

T

I

A

A

Q

Y

T

E

I

I

D

D

K

Q

E

E

V

I

R

Q

R

R

L

I

V

I

D

K

E

E

G

C

Y

Y

V

E

E

R

A

A

K

K

R

Q

I

I

L

L

S

T

E

E

K

N

A

R

V

D

D

K

L

L

E

E

T

L

L

I

A

A

R

Q

G

T

L

L

L

L

E

K

Y

V

E

E

T

T

L

L

T

D

G

A

D

E

E

Q

I

I

V

K

D

H

L

L

L

I

N

D

-

H

G

G

K

T

P

L

P

P

V

E

R

R

D

N

K207 (= K202) mutation to N: Does not complement an ftsH deletion, loss of ATPase activity.
E424 (= E419) mutation to Q: Does not complement an ftsH deletion, loss of protease activity against casein.

Q5SI82 ATP-dependent zinc metalloprotease FtsH; EC 3.4.24.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
52% identity, 93% coverage: 8:601/640 of query aligns to 6:595/624 of Q5SI82

query
sites
Q5SI82

F

F

A

S

L

L

W

L

V

A

I

L

I

V

V

L

-

G

L

L

L

A

L

F

L

L

A

A

L

W

-

A

F

F

S

S

L

L

F

A

Q

G

S

T

P

V

G

G

Q

-

R

-

S

A

S

P

A

S

N

G

D

T

I

V

S

N

F

Y

S

T

Q

T

L

F

L

L

S

E

D

D

V

L

D

K

Q

A

G

G

R

R

V

V

R

K

D

E

V

V

V

V

I

V

E

R

G

A

P

G

N

D

-

T

-

R

I

I

S

Q

G

G

S

V

F

L

T

E

D

D

G

G

R

S

Q

A

F

F

Q

T

T

T

Y

Y

A

A

-

A

-

S

P

P

S

P

D

D

P

N

T

A

L

T

V

L

Q

E

R

G

L

W

Y

M

G

A

K

R

G

G

V

V

S

S

I

V

T

R

A

V

R

E

P

P

P

P

S

Q

-

G

D

Q

N

N

V

A

P

L

W

G

F

F

V

L

S

W

L

P

L

L

V

L

S

L

W

-

L

-

P

-

F

V

I

G

A

L

L

L

I

I

G

G

V

A

W

L

I

Y

F

Y

L

F

S

S

R

R

Q

N

M

G

Q

R

-

A

G

G

A

P

G

S

G

D

K

S

A

A

M

F

G

S

F

F

G

T

K

K

S

S

R

R

A

A

K

R

L

V

L

L

T

T

E

E

A

A

H

P

G

-

R

K

V

V

T

T

F

F

E

K

D

D

V

V

A

A

G

G

I

A

D

E

E

E

A

A

K

K

S

E

D

E

L

L

T

K

E

E

I

I

V

V

D

E

F

F

L

L

R

K

D

N

P

P

Q

S

K

R

F

F

Q

H

R

E

L

M

G

G

G

A

R

R

I

I

P

P

R

K

G

G

V

V

L

L

L

L

V

V

G

G

P

P

P

P

G

G

T

V

G

G

K

K

T

T

L

H

L

L

A

A

R

R

A

A

I

V

A

A

G

G

E

E

A

A

N

R

V

V

P

P

F

F

F

I

T

T

I

A

S

S

G

G

S

S

D

D

F

F

V

V

E

E

M

M

F

F

V

V

G

G

V

V

G

G

A

A

S

A

R

R

V

V

R

R

D

D

M

L

F

F

E

E

Q

T

A

A

K

K

K

R

N

H

A

A

P

P

C

C

I

I

I

V

F

F

I

I

D

D

E

E

I

I

D

D

A

A

V

V

G

G

R

R

H

K

R

R

G

G

A

S

G

G

L

V

G

G

G

G

G

G

N

N

D

D

E

E

R

R

E

E

Q

Q

T

T

L

L

N

N

Q

Q

L

L

L

L

V

V

E

E

M

M

D

D

G

G

F

F

E

E

A

K

N

D

E

T

G

A

I

I

I

V

L

V

I

M

A

A

A

A

T

T

N

N

R

R

P

P

D

D

V

I

L

L

D

D

P

P

A

A

L

L

L

L

R

R

P

P

G

G

R

R

F

F

D

D

R

R

Q

Q

V

I

V

A

V

I

P

D

N

A

P

P

D

D

V

V

V

K

G

G

R

R

E

E

Q

Q

I

I

L

L

K

R

V

I

H

H

A

A

R

R

K

G

I

K

P

P

I

L

A

A

P

E

D

D

V

V

N

D

L

L

K

A

V

L

I

L

A

A

R

K

G

R

T

T

P

P

G

G

F

F

S

V

G

G

A

A

D

D

L

L

A

E

N

N

L

L

C

L

N

N

E

E

A

A

A

A

L

L

M

L

A

A

A

A

R

R

R

E

N

G

K

R

R

R

M

K

V

I

T

T

M

M

V

K

E

D

F

L

E

E

D

E

A

A

K

A

D

D

K

R

V

V

M

M

M

M

G
|
G

A

P

E

A

R

K

R

K

S

S

L

L

V

V

M

L

T

S

E

P

E

R

E

D

K

R

M

R

L

I

T

T

A

A

Y

Y

H

H

E

E

G

A

G

G

H

H

A

A

I

L

V

A

A

A

L

H

N

F

V

L

P

E

A

H

T

A

D

D

P

G

V

V

H

H

K

K

A

V

T

T

I

I

I

V

P

P

R

R

G

G

R

R

A

A

L

L

G

G

M

F

V

M

M

M

Q

-

L

-

P

P

E

R

R

R

-

E

D

D

K

M

L

L

S

H

M

W

S

S

Y

R

E

K

Q

R

M

L

T

L

S

D

R

Q

L

I

A

A

I

V

M

A

M

L

G

A

G

G

R

R

V

A

A

A

E

E

E

E

L

I

I

V

F

F

G

-

H

-

D

D

K

D

V

V

T

T

S

T

G

G

A

A

A

E

S

N

D

D

I

F

E

R

Q

Q

A

A

T

T

R

E

L

L

A

A

K

R

M

R

M

M

V

I

T

T

R

E

W

W

G

G

F

M

S

H

D

P

E

E

L

F

G

G

Q

P

V

V

A

A

Y

Y

G

A

E

V

N

R

Q

E

D

D

E

T

V

Y

F

L

L

G

G

G

M

Y

S

D

M

V

G

-

R

-

T

-

Q

R

N

Q

V

Y

S

S

E

E

A

E

T

T

A

A

Q

K

T

R

I

I

D

D

K

E

E

A

V

V

R

R

R

R

L

L

V

I

D

E

E

E

G

Q

Y

Y

V

Q

E

R

A

V

K

K

R

A

I

L

L

L

S

L

E

E

K

K

A

R

V

E

D

V

L

L

E

E

T

R

L

V

A

A

R

E

G

T

L

L

L

L

E

E

Y

R

E

E

T

T

L

L

T

T

G

A

D

E

E

E

I

F

V

Q

D

R

L

V

L

V

N

E

G

G

K

L

P

P

G399 (= G399) mutation to L: No effect on protease activity against alpha-casein.

3kdsE Apo-ftsh crystal structure (see paper)
57% identity, 70% coverage: 152:598/640 of query aligns to 2:426/427 of 3kdsE

query
sites
3kdsE

R

R

V

V

T

T

F

F

E

K

D

D

V

V

A

G

G

G

I

A

D

E

E

E

A

A

K

I

S

E

D

E

L

L

T

K

E

E

I

V

V

V

D

E

F

F

L

L

R

K

D

D

P

P

Q

S

K

K

F

F

Q

N

R

R

L

I

G

G

G

A

R

R

I

M

P

P

R

K

G

G

V

I

L

L

L

L

V

V

G

G

P

P

P

P

G

G

T

T

G

G

K

A

T

T

L

L

L

L

A

A

R

R

A

A

I

V

A

A

G

G

E

E

A

A

N

N

V

V

P

P

F

F

F

F

T

H

I

I

S

S

G

G

S

S

D

D

F

F

V

V

E

E

M

L

F

F

V

V

G

G

V

V

G

G

A

A

S

A

R

R

V

V

R

R

D

D

M

L

F

F

E

A

Q

Q

A

A

K

K

K

A

N

H

A

A

P

P

C

C

I

I

I

V

F

F

I

I

D

D

E

E

I

I

D

D

A

A

V

V

G

G

R

R

H

H

R

-

G

-

A

-

G

-

L

-

G

-

G

-

G

-

N

-

D

D

E

E

R

R

E

E

Q

Q

T

T

L

L

N

N

Q

Q

L

L

L

L

V

V

E

E

M

M

D

D

G

G

F

F

E

D

A

S

N

K

E

E

G

G

I

I

I

I

L

V

I

M

A

A

A

A

T

T

N

N

R

R

P

P

D

D

V

I

L

L

D

D

P

P

A

A

L

L

L

L

R

R

P

P

G

G

R

R

F

F

D

D

R

K

Q

K

V

I

V

V

V

V

P

D

N

P

P

P

D

D

V

M

V

L

G

G

R

R

E

K

Q

K

I

I

L

L

K

E

V

I

H

H

A

T

R

R

K

N

I

K

P

P

I

L

A

A

P

E

D

D

V

V

N

N

L

L

K

E

V

I

I

I

A

A

R

K

G

R

T

T

P

P

G

G

F

F

S

V

G

G

A

A

D

D

L

L

A

E

N

N

L

L

C

V

N

N

E

E

A

A

A

A

L

L

M

L

A

A

A

A

R

R

R

E

N

G

K

R

R

D

M

K

V

I

T

T

M

M

V

K

E

D

F

F

E

E

D

E

A

A

K

I

D

D

K

R

V

V

M

I

M

A

G

G

A

P

E

A

R

R

R

K

S

S

L

L

V

L

M

I

T

S

E

P

E

A

E

E

K

K

M

R

L

I

T

I

A

A

Y

Y

H
|
H

E
|
E

G

A

G

G

H
|
H

A

A

I

V

V

V

A

S

L

T

N

V

V

V

P

P

A

N

T

G

D

E

P

P

V

V

H

H

K

R

A

I

T

S

I

I

I

I

P

P

R

R

G

G

-

Y

R
x
K

A

A

L
|
L

G

G

M

Y

V

T

M

L

Q

H

L

L

P

P

E

E

R

E

D

D

K

K

L

Y

S

L

M

V

S

S

Y

R

E

N

Q

E

M

L

T

L

S

D

R

K

L

L

A

T

I

A

M

L

M

L

G

G

G

G

R

R

V

A

A

A

E

E

E

E

L

V

I

V

F

F

G

G

H

-

D

-

K

D

V

V

T

T

S
|
S

G

G

A

A

A
|
A

S

N

D
|
D

I

I

E

E

Q

R

A

A

T

T

R

E

L

I

A

A

K

R

M

N

M

M

V

V

T

C

R

Q

W

L

G

G

F

M

S

S

D

E

E

E

L

L

G

G

Q

P

V

L

A

A

Y

W

G

G

E

K

N

-

Q

-

D

-

E

-

V

-

F

-

L

-

G

-

M

-

S

-

M

-

G

-

R

R

T

L

Q

R

N

N

V

Y

S

S

E

E

A

E

T

V

A

A

Q

S

T

K

I

I

D

D

K

E

E

E

V

V

R

K

R

K

L

I

V

V

D

T

E

N

G

C

Y

Y

V

E

E

R

A

A

K

K

R

E

I

I

L

I

S

R

E

K

K

Y

A

R

V

K

D

Q

L

L

E

D

T

N

L

I

A

V

R

E

G

I

L

L

L

L

E

E

Y

K

E

E

T

T

L

I

T

E

G

G

D

D

E

E

I

L

V

R

D

R

L

I

L

L

N

S

binding N-{(2R)-2-[2-(hydroxyamino)-2-oxoethyl]-4-methylpentanoyl}-3-naphthalen-2-yl-L-alanyl-L-alaninamide: H259 (= H418), E260 (= E419), H263 (= H422), K287 (≠ R445), L289 (= L447), S331 (= S491), A334 (= A494), D336 (= D496)
binding zinc ion: H259 (= H418), H263 (= H422), D336 (= D496)

7wi4B Cryo-em structure of e.Coli ftsh protease cytosolic domains (see paper)
56% identity, 72% coverage: 145:602/640 of query aligns to 1:407/410 of 7wi4B

query
sites
7wi4B

L

M

L

L

T

T

E

E

A

D

H

Q

G

I

R

K

V

T

T

T

F

F

E

A

D

D

V
|
V

A
|
A

G

G

I

C

D

D

E

E

A

A

K

K

S

E

D

E

L

V

T

A

E

E

I

L

V

V

D

E

F

Y

L

L

R

R

D

E

P

P

Q

S

K

R

F

F

Q

Q

R

K

L

L

G

G

G

G

R

K

I

I

P

P

R

K

G

G

V

V

L

L

L

M

V

V

G

G

P

P

P
|
P

G
|
G

T

T

G
|
G

K
|
K

T
|
T

L
|
L

L

L

A

A

R

K

A

A

I

I

A

A

G

G

E

E

A

A

N

K

V

V

P

P

F

F

F

F

T

T

I

I

S

S

G

G

S

-

D

-

F

-

V

-

E

-

M

-

F

-

V

-

G

-

V

-

G

-

A
|
A

S
|
S

R

R

V

V

R

R

D

D

M

M

F

F

E

E

Q

Q

A

A

K

K

K

K

N

A

A

A

P

P

C

C

I

I

I

I

F

F

I

I

D

D

E

Q

I

I

D

-

A

-

V

-

G

-

R

-

H

-

R

-

G

-

A

-

G

-

L

-

G

-

G

-

G

-

N

-

D

-

E

-

R

R

E

E

Q

Q

T

T

L

L

N

N

Q

Q

L

M

L

L

V

V

E

E

M

M

D

D

G

G

F

F

E

E

A

G

N

N

E

E

G

G

I

I

I

I

L

V

I

I

A

A

A

A

T

T

N

N

R

R

P

P

D

-

V

-

L

-

D

-

P

-

A

-

L

-

L

-

R

-

P

-

G

G

R

R

F

F

D

D

R

R

Q

Q

V

V

V

V

V

V

P

G

N

L

P

P

D

D

V

V

V

R

G

G

R

R

E

E

Q

Q

I

I

L

L

K

K

V

V

H

H

A

M

R

R

K

R

I

V

P

P

I

L

A

A

P

P

D

D

V

I

N

D

L

A

K

A

V

I

I

I

A

A

R

R

G

G

T

T

P

P

G

G

F

F

S

S

G

G

A

A

D

D

L

L

A

A

N

N

L

L

C

V

N

N

E

E

A

A

A

A

L

L

M

F

A

A

A

A

R

R

R

G

N

N

K

K

R

R

M

V

V

V

T

S

M

M

V

V

E

E

F

F

E

E

D

K

A

A

K

K

D

D

K

K

V

I

M

M

M

M

G

G

A

A

E

E

R

R

R

R

S

S

L

M

V

V

M

M

T

T

E

E

E

A

E

Q

K

K

M

E

L

S

T

T

A

A

Y

Y

H
|
H

E

Q

G

A

G

G

H
|
H

A

A

I

I

V

I

A

G

L

R

N

L

V

V

P

P

A

E

T

H

D

D

P

P

V

V

H

H

K

K

A

V

T

T

I

I

I

I

P

P

R

R

G

G

R

R

A

A

L

L

G

G

M

V

V

T

M

F

Q

F

L

L

P

P

E

E

R

G

D

D

K

A

L

I

S

S

M

A

S

S

Y

R

E

Q

Q

K

M

L

T

E

S

S

R

Q

L

I

A

S

I

T

M

L

M

Y

G

G

G

G

R

R

V

L

A

A

E

E

E

E

L

I

I

I

F

Y

G

G

H

P

D

E

K

H

V

V

T

S

S

T

G

G

A

A

A

S

S

N

D
|
D

I

I

E

K

Q

V

A

A

T

T

R

N

L

L

A

A

K

R

M

N

M

M

V

V

T

T

R

Q

W

W

G

G

F

F

S

S

D

E

E

K

L

L

G

G

Q

P

V

L

A

L

Y

Y

G

A

E

H

N

M

Q

S

D

D

E

E

V

-

F

-

L

-

G

-

M

-

S

-

M

-

G

-

R

-

T

-

Q

-

N

-

V

-

S

-

E

-

A

-

T

T

A

A

Q

R

T

I

I

I

D

D

K

Q

E

E

V

V

R

K

R

A

L

L

V

I

D

E

E

R

G

N

Y

Y

V

N

E

R

A

A

K

R

R

Q

I

L

L

L

S

T

E

D

K

N

A

M

V

D

D

I

L

L

E

H

T

A

L

M

A

K

R

D

G

A

L

L

L

M

E

K

Y

Y

E

E

T

T

L

I

T

D

G

A

D

P

E

Q

I

I

V

D

D

D

L

L

L

M

-

A

-

R

-

R

N

D

G

V

K

R

P

P

P

P

binding phosphoaminophosphonic acid-adenylate ester: V14 (= V158), A15 (= A159), P54 (= P198), G55 (= G199), G57 (= G201), K58 (= K202), T59 (= T203), L60 (= L204)
binding magnesium ion: A79 (= A234), S80 (= S235)
binding zinc ion: H236 (= H418), H240 (= H422), D314 (= D496)

7wi4A Cryo-em structure of e.Coli ftsh protease cytosolic domains (see paper)
56% identity, 72% coverage: 145:602/640 of query aligns to 1:407/410 of 7wi4A

query
sites
7wi4A

L

M

L

L

T

T

E

E

A

D

H

Q

G

I

R

K

V

T

T

T

F

F

E

A

D

D

V

V

A
|
A

G

G

I

C

D

D

E

E

A

A

K

K

S

E

D

E

L

V

T

A

E

E

I

L

V

V

D

E

F

Y

L

L

R

R

D

E

P

P

Q

S

K

R

F

F

Q

Q

R

K

L

L

G

G

G

G

R

K

I

I

P

P

R

K

G

G

V

V

L

L

L

M

V

V

G

G

P

P

P
|
P

G
|
G

T
|
T

G
|
G

K
|
K

T
|
T

L
|
L

L

L

A

A

R

K

A

A

I

I

A

A

G

G

E

E

A

A

N

K

V

V

P

P

F

F

F

F

T

T

I

I

S

S

G

G

S

-

D

-

F

-

V

-

E

-

M

-

F

-

V

-

G

-

V

-

G

-

A

A

S

S

R

R

V

V

R

R

D

D

M

M

F

F

E

E

Q

Q

A

A

K

K

K

K

N

A

A

A

P

P

C

C

I

I

I

I

F

F

I

I

D

D

E

Q

I

I

D

-

A

-

V

-

G

-

R

-

H

-

R

-

G

-

A

-

G

-

L

-

G

-

G

-

G

-

N

-

D

-

E

-

R

R

E

E

Q

Q

T

T

L

L

N

N

Q

Q

L

M

L

L

V

V

E

E

M

M

D

D

G

G

F

F

E

E

A

G

N

N

E

E

G

G

I

I

I

I

L

V

I

I

A

A

A

A

T

T

N

N

R

R

P

P

D

-

V

-

L

-

D

-

P

-

A

-

L

-

L

-

R

-

P

-

G

G

R

R

F

F

D

D

R

R

Q

Q

V

V

V

V

V

V

P

G

N

L

P

P

D

D

V

V

V

R

G

G

R

R

E

E

Q

Q

I

I

L

L

K

K

V

V

H
|
H

A

M

R

R

K

R

I

V

P

P

I

L

A

A

P

P

D

D

V

I

N

D

L

A

K

A

V

I

I

I

A

A

R

R

G

G

T

T

P

P

G

G

F

F

S

S

G

G

A

A

D

D

L

L

A

A

N

N

L

L

C

V

N

N

E

E

A

A

A

A

L

L

M

F

A

A

A

A

R

R

R

G

N

N

K

K

R

R

M

V

V

V

T

S

M

M

V

V

E

E

F

F

E

E

D

K

A

A

K

K

D

D

K

K

V

I

M

M

M

M

G

G

A

A

E

E

R

R

R

R

S

S

L

M

V

V

M

M

T

T

E

E

E

A

E

Q

K

K

M

E

L

S

T

T

A

A

Y

Y

H
|
H

E

Q

G

A

G

G

H
|
H

A

A

I

I

V

I

A

G

L

R

N

L

V

V

P

P

A

E

T

H

D

D

P

P

V

V

H

H

K

K

A

V

T

T

I

I

I

I

P

P

R

R

G

G

R

R

A

A

L

L

G

G

M

V

V

T

M

F

Q

F

L

L

P

P

E

E

R

G

D

D

K

A

L

I

S

S

M

A

S

S

Y

R

E

Q

Q

K

M

L

T

E

S

S

R

Q

L

I

A

S

I

T

M

L

M

Y

G

G

G

G

R

R

V

L

A

A

E

E

E

E

L

I

I

I

F

Y

G

G

H

P

D

E

K

H

V

V

T

S

S

T

G

G

A

A

A

S

S

N

D
|
D

I

I

E

K

Q

V

A

A

T

T

R

N

L

L

A

A

K

R

M

N

M

M

V

V

T

T

R

Q

W

W

G

G

F

F

S

S

D

E

E

K

L

L

G

G

Q

P

V

L

A

L

Y

Y

G

A

E

H

N

M

Q

S

D

D

E

E

V

-

F

-

L

-

G

-

M

-

S

-

M

-

G

-

R

-

T

-

Q

-

N

-

V

-

S

-

E

-

A

-

T

T

A

A

Q

R

T

I

I

I

D

D

K

Q

E

E

V

V

R

K

R

A

L

L

V

I

D

E

E

R

G

N

Y

Y

V

N

E

R

A

A

K

R

R

Q

I

L

L

L

S

T

E

D

K

N

A

M

V

D

D

I

L

L

E

H

T

A

L

M

A

K

R

D

G

A

L

L

L

M

E

K

Y

Y

E

E

T

T

L

I

T

D

G

A

D

P

E

Q

I

I

V

D

D

D

L

L

L

M

-

A

-

R

-

R

N

D

G

V

K

R

P

P

P

P

binding phosphoaminophosphonic acid-adenylate ester: A15 (= A159), P54 (= P198), G55 (= G199), T56 (= T200), G57 (= G201), K58 (= K202), T59 (= T203), L60 (= L204), H156 (= H338)
binding zinc ion: H236 (= H418), H240 (= H422), D314 (= D496)

Q9FGM0 ATP-dependent zinc metalloprotease FTSH 11, chloroplastic/mitochondrial; AtFTSH11; EC 3.4.24.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
52% identity, 78% coverage: 96:597/640 of query aligns to 289:784/806 of Q9FGM0

query
sites
Q9FGM0

P

P

P

K

S

V

D

S

N

N

V

K

P

S

W

R

F

F

V

A

S

Q

L

E

L

L

V

V

S

S

W

T

L

I

P

L

F

F

I

T

A

V

L

A

I

V

G

G

-

L

V

V

W

W

I

I

F

M

L

G

S

A

R

A

Q

A

M

L

Q

Q

G

K

A

Y

G

I

G

G

K

S

A

L

M

G

G

G

F

I

G

G

-

T

-

S

-

G

-

V

-

G

-

S

-

S

-

S

-

S

-

Y

-

S

-

P

K

K

S

E

R

L

A

N

K

K

L

E

L

I

T

T

E

P

A

E

H

K

G

N

R

V

V

K

T

T

F

F

E

K

D

D

V

V

A

K

G

G

I

C

D

D

E

D

A

A

K

K

S

Q

D

E

L

L

T

E

E

E

I

V

V

V

D

E

F

Y

L

L

R

K

D

N

P

P

Q

S

K

K

F

F

Q

T

R

R

L

L

G

G

G

G

R

K

I

L

P

P

R

K

G

G

V

I

L

L

L

L

V

T

G

G

P

A

P

P

G

G

T

T

G

G

K

K

T

T

L

L

L

L

A

A

R

K

A

A

I

I

A

A

G

G

E

E

A

A

N

G

V

V

P

P

F

F

F

F

T

Y

I

R

S

A

G

G

S

S

D

E

F

F

V

E

E

E

M

M

F

F

V

V

G

G

V

V

G

G

A

A

S

R

R

R

V

V

R

R

D

S

M

L

F

F

E

Q

Q

A

A

A

K

K

K

K

N

K

A

A

P

P

C

C

I

I

I

I

F

F

I

I

D

D

E

E

I

I

D

D

A

A

V

V

G

G

R

S

H

T

R

R

G

K

A

Q

G

W

L

E

G

G

G

-

G

-

N

-

D

-

E

H

R

T

E

K

Q

K

T

T

L

L

N

H

Q

Q

L

L

L

L

V

V

E

E

M

M

D

D

G

G

F

F

E

E

A

Q

N

N

E

E

G

G

I

I

I

I

L

V

I

M

A

A

A

A

T

T

N

N

R

L

P

P

D

D

V

I

L

L

D

D

P

P

A

A

L

L

L

T

R

R

P

P

G

G

R

R

F

F

D

D

R

R

Q

H

V

I

V

V

V

V

P

P

N

S

P

P

D

D

V

V

V

R

G

G

R

R

E

E

Q

E

I

I

L

L

K

E

V

L

H

Y

A

L

R

Q

K

G

I

K

P

P

I

M

A

S

P

E

D

D

V

V

N

D

L

V

K

K

V

A

I

I

A

A

R

R

G

G

T

T

P

P

G

G

F

F

S

N

G
|
G

A

A

D

D

L

L

A

A

N

N

L

L

C

V

N

N

E

I

A

A

A

A

L

I

M

K

A

A

A

A

R

V

R

E

N

G

K

A

R

E

M

K

V

L

T

S

M

S

V

E

E

Q

F

L

E

E

D

F

A

A

K

K

D

D

K

R

V

I

M

V

M

M

G

G

A

T

E

E

R

R

R

K

S

T

L

M

V

F

M

V

T

S

E

E

E

D

E

S

K

K

M

K

L

L

T

T

A

A

Y

Y

H

H

E

E

G

S

G

G

H

H

A

A

I

I

V

V

A

A

L

L

N

N

V

T

P

K

A

G

T

A

D

H

P

P

V

I

H

H

K

K

A

A

T

T

I

I

I

M

P

P

R

R

G

G

R

S

A

A

L

L

G

G

M

M

V

V

M

T

Q

Q

L

L

P

P

E

S

R

N

D

D

K

E

L

T

S

S

M

V

S

S

Y

K

E

R

Q

Q

M

L

T

L

S

A

R

R

L

L

A

D

I

V

M

C

M

M

G

G

G

G

R

R

V

V

A

A

E

E

E

E

L

L

I

I

F

F

G

G

H

L

D

D

K

H

V

I

T

T

S

T

G

G

A

A

A

S

S

S

D

D

I

L

E

S

Q

Q

A

A

T

T

R

E

L

L

A

A

K

Q

M

Y

M

M

V

V

T

S

R

S

W

C

G

G

F

M

S

S

D

E

E

A

L

I

G

G

Q

P

V

V

A

H

Y

I

G

K

E

E

N

R

Q

P

D

-

E

-

V

-

F

-

L

-

G

-

M

-

S

-

M

-

G

-

R

-

T

-

Q

-

N

-

V

-

S

S

E

S

A

D

T

M

A

Q

Q

S

T

R

I

I

D

D

K

A

E

E

V

V

R

V

R

K

L

L

V

L

D

R

E

E

G

A

Y

Y

V

E

E

R

A

V

K

K

R

S

I

L

L

L

S

K

E

R

K

H

A

E

V

K

D

Q

L

L

E

H

T

T

L

L

A

A

R

N

G

A

L

L

L

L

E

E

Y

Y

E

E

T

T

L

L

T

T

G

A

D

E

E

D

I

I

V

K

D

R

L

I

L

L

G564 (= G362) mutation to A: In atts244; loss of thermotolerance.

2dhrA Whole cytosolic region of atp-dependent metalloprotease ftsh (g399l) (see paper)
58% identity, 72% coverage: 142:601/640 of query aligns to 1:453/458 of 2dhrA

query
sites
2dhrA

R

R

A

A

K

R

L

V

L

L

T

T

E

E

A

A

H

-

G

P

R

K

V

V

T

T

F

F

E

K

D

D

V

V

A

A

G

G

I

A

D

E

E

E

A

A

K

K

S

E

D

E

L

L

T

K

E

E

I

I

V

V

D

E

F

F

L

L

R

K

D

N

P

P

Q

S

K

R

F

F

Q

H

R

E

L

M

G

G

G

A

R

R

I

I

P

P

R

K

G

G

V

V

L

L

L

L

V

V

G

G

P

P

P

P

G
|
G

T
x
V

G
|
G

K
|
K

T
|
T

L
x
H

L

L

A

A

R

R

A

A

I

V

A

A

G

G

E

E

A

A

N

R

V

V

P

P

F

F

F

I

T

T

I

A

S

S

G

G

S

S

D

D

F

F

V

V

E

E

M

M

F

F

V

V

G

G

V

V

G

G

A

A

S

A

R

R

V

V

R

R

D

D

M

L

F

F

E

E

Q

T

A

A

K

K

K

R

N

H

A

A

P

P

C

C

I

I

I

V

F

F

I

I

D

D

E

E

I

I

D

D

A

A

V

V

G

G

R

R

H

K

R

R

G

G

A

S

G

G

L

V

G

G

G

G

G

G

N

N

D

D

E

E

R

R

E

E

Q

Q

T

T

L

L

N

N

Q

Q

L

L

L

L

V

V

E

E

M

M

D

D

G

G

F

F

E

E

A

K

N

D

E

T

G

A

I

I

I

V

L

V

I

M

A

A

A

A

T

T

N

N

R

R

P

P

D

D

V

I

L

L

D

D

P

P

A

A

L

L

L

L

R

R

P

P

G

G

R

R

F

F

D

D

R

R

Q

Q

V

I

V

A

V

I

P

D

N

A

P

P

D

D

V

V

V

K

G

G

R

R

E

E

Q

Q

I
|
I

L

L

K

R

V

I

H
|
H

A

A

R

R

K

G

I

K

P

P

I

L

A

A

P

E

D

D

V

V

N

D

L

L

K

A

V

L

I

L

A

A

R

K

G

R

T

T

P

P

G

G

F

F

S

V

G

G

A

A

D

D

L

L

A

E

N

N

L

L

C

L

N

N

E

E

A

A

A

A

L

L

M

L

A

A

A

A

R

R

R

E

N

G

K

R

R

R

M

K

V

I

T

T

M

M

V

K

E

D

F

L

E

E

D

E

A

A

K

A

D

D

K

R

V

V

M

M

M

M

G

L

A

P

E

A

R

K

R

K

S

S

L

L

V

V

M

L

T

S

E

P

E

R

E

D

K

R

M

R

L

I

T

T

A

A

Y

Y

H

H

E

E

G

A

G

G

H

H

A

A

I

L

V

A

A

A

L

H

N

F

V

L

P

E

A

H

T

A

D

D

P

G

V

V

H

H

K

K

A

V

T

T

I

I

I

V

P

P

R

R

G

G

R

R

A

A

L

L

G

G

M

F

V

M

M

M

Q

-

L

-

P

P

E

R

R

R

-

E

D

D

K

M

L

L

S

H

M

W

S

S

Y

R

E

K

Q

R

M

L

T