SitesBLAST
Comparing WP_012170593.1 NCBI__GCF_000010525.1:WP_012170593.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
24% identity, 100% coverage: 1:416/417 of query aligns to 26:400/824 of Q8GAI3
- W66 (= W55) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
- H67 (≠ A56) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
23% identity, 96% coverage: 3:403/417 of query aligns to 4:348/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (≠ L6), G8 (= G7), G10 (= G9), V11 (= V10), I12 (= I11), V30 (≠ L29), E31 (≠ D30), K32 (≠ R31), E38 (= E38), A39 (≠ T39), S40 (= S40), A43 (= A64), G45 (≠ K66), L46 (≠ W67), V171 (≠ L224), G200 (≠ L253), G201 (= G254), W203 (≠ Y256), G298 (= G354), R300 (= R356), P301 (= P357), Y326 (≠ G381), R327 (≠ T382), N328 (≠ L383), G329 (= G384), I330 (≠ W385)
Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
25% identity, 71% coverage: 121:416/417 of query aligns to 79:370/377 of Q5L2C2
- V180 (≠ L224) binding FAD
- R309 (= R356) binding substrate
- 334:340 (vs. 380:386, 29% identical) binding FAD
- R336 (≠ T382) binding substrate
Sites not aligning to the query:
- 14:15 binding FAD
- 34:35 binding FAD
- 42:43 binding FAD
- 47:49 binding FAD
4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
24% identity, 71% coverage: 121:416/417 of query aligns to 78:368/370 of 4yshA
- active site: I262 (≠ V311), L283 (= L332), G305 (= G354), N335 (≠ L383), L338 (≠ T386)
- binding flavin-adenine dinucleotide: V178 (≠ L224), S206 (≠ L253), G207 (= G254), W209 (≠ Y256), R307 (= R356), H332 (= H380), R334 (≠ T382), N335 (≠ L383), G336 (= G384), I337 (≠ W385)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48
4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
24% identity, 71% coverage: 121:416/417 of query aligns to 78:368/368 of 4yshB
- active site: I262 (≠ V311), L283 (= L332), G305 (= G354), N335 (≠ L383), L338 (≠ T386)
- binding flavin-adenine dinucleotide: V178 (≠ L224), S206 (≠ L253), W209 (≠ Y256), R307 (= R356), H332 (= H380), R334 (≠ T382), N335 (≠ L383), G336 (= G384), I337 (≠ W385), L338 (≠ T386)
- binding glycine: G249 (≠ Y298), Y251 (≠ I300), Y251 (≠ I300), A264 (≠ G313), R307 (= R356), R334 (≠ T382), R334 (≠ T382)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48
3gsiA Crystal structure of d552a dimethylglycine oxidase mutant of arthrobacter globiformis in complex with tetrahydrofolate (see paper)
22% identity, 100% coverage: 2:417/417 of query aligns to 3:391/827 of 3gsiA
- active site: H222 (≠ K274), Y256 (= Y297)
- binding flavin-adenine dinucleotide: G10 (= G9), I11 (≠ V10), V12 (≠ I11), D32 (= D30), Q33 (≠ R31), G41 (≠ Y51), S42 (= S52), T43 (≠ S53), H45 (= H72), P47 (= P74), L49 (≠ I76), T170 (≠ D223), V171 (≠ L224), A200 (≠ L253), G201 (= G254), W203 (≠ Y256), H222 (≠ K274), Y256 (= Y297), G330 (= G354), I331 (≠ L355), F332 (≠ R356), V357 (≠ L383), W358 (≠ G384), V359 (≠ W385), T360 (= T386)
- binding magnesium ion: D254 (vs. gap)
Sites not aligning to the query:
- active site: 549
- binding magnesium ion: 409
- binding (6s)-5,6,7,8-tetrahydrofolate: 505, 536, 551, 563, 629, 648, 655, 696
1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
22% identity, 100% coverage: 2:417/417 of query aligns to 4:392/828 of 1pj6A
- active site: H223 (≠ K274), Y257 (= Y297)
- binding flavin-adenine dinucleotide: G9 (= G7), G11 (= G9), I12 (≠ V10), V13 (≠ I11), D33 (= D30), Q34 (≠ R31), G42 (≠ Y51), S43 (= S52), T44 (≠ S53), H46 (= H72), P48 (= P74), L50 (≠ I76), V172 (≠ L224), A201 (≠ L253), G202 (= G254), W204 (≠ Y256), H223 (≠ K274), Y257 (= Y297), G331 (= G354), I332 (≠ L355), V358 (≠ L383), W359 (≠ G384), V360 (≠ W385), T361 (= T386)
Sites not aligning to the query:
1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
22% identity, 100% coverage: 2:417/417 of query aligns to 3:391/827 of 1pj7A
- active site: H222 (≠ K274), Y256 (= Y297)
- binding flavin-adenine dinucleotide: G8 (= G7), G10 (= G9), I11 (≠ V10), V12 (≠ I11), D32 (= D30), Q33 (≠ R31), G41 (≠ Y51), S42 (= S52), T43 (≠ S53), H45 (= H72), P47 (= P74), L49 (≠ I76), T170 (≠ D223), V171 (≠ L224), A200 (≠ L253), G201 (= G254), W203 (≠ Y256), H222 (≠ K274), Y256 (= Y297), I331 (≠ L355), V357 (≠ L383), W358 (≠ G384), V359 (≠ W385), T360 (= T386)
Sites not aligning to the query:
- active site: 549
- binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: 505, 536, 549, 551, 563, 629, 648, 655, 696
Q9AGP8 Dimethylglycine oxidase; DMGO; EC 1.5.3.10 from Arthrobacter globiformis (see 2 papers)
22% identity, 100% coverage: 2:417/417 of query aligns to 6:394/830 of Q9AGP8
- IV 14:15 (≠ VI 10:11) binding FAD
- DQ 35:36 (≠ DR 30:31) binding FAD
- ST-----------------SH 45:48 (≠ SSPWAAPGIPMKAAKWLFMKH 52:72) binding FAD
- L52 (≠ I76) binding FAD
- V174 (≠ L224) binding FAD
- H225 (≠ K274) Important for catalytic activity; mutation to Q: Reduces catalytic efficiency 3-fold and substrate affinity 30-fold.
- Y259 (= Y297) Important for catalytic activity; binding FAD; mutation to F: Reduces catalytic efficiency 225-fold and substrate affinity 25-fold.
- VWVT 360:363 (≠ LGWT 383:386) binding FAD
Sites not aligning to the query:
- 539 binding (6S)-5,6,7,8-tetrahydrofolate
- 552 Important for catalytic activity; D→A: No effect on the activity.; D→N: Reduces activity 3-fold.
O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
25% identity, 55% coverage: 169:399/417 of query aligns to 122:346/369 of O31616
- V174 (≠ L224) binding FAD
- H244 (≠ Y298) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
- R302 (= R356) binding substrate
- 327:333 (vs. 380:386, 29% identical) binding FAD
- R329 (≠ T382) binding substrate
Sites not aligning to the query:
- 14:15 binding FAD
- 34:35 binding FAD
- 42:43 binding FAD
- 47:49 binding FAD
- 51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
- 54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
25% identity, 55% coverage: 169:399/417 of query aligns to 122:346/364 of 1ng3A
- binding acetylamino-acetic acid: Y246 (≠ I300), R302 (= R356), R329 (≠ T382)
- binding flavin-adenine dinucleotide: V174 (≠ L224), S202 (≠ L253), G203 (= G254), W205 (≠ Y256), F209 (= F260), G300 (= G354), R302 (= R356), H327 (= H380), R329 (≠ T382), N330 (≠ L383), G331 (= G384), I332 (≠ W385)
- binding phosphate ion: R254 (= R308)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49
- binding phosphate ion: 89
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
25% identity, 55% coverage: 169:399/417 of query aligns to 122:346/364 of 3if9A
- binding flavin-adenine dinucleotide: P173 (≠ D223), V174 (≠ L224), S202 (≠ L253), G203 (= G254), W205 (≠ Y256), F209 (= F260), G300 (= G354), R302 (= R356), H327 (= H380), F328 (≠ G381), R329 (≠ T382), N330 (≠ L383), G331 (= G384), I332 (≠ W385)
- binding glycolic acid: Y246 (≠ I300), R302 (= R356), R329 (≠ T382)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49
2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
24% identity, 78% coverage: 91:417/417 of query aligns to 59:388/403 of 2gagB