SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012172510.1 NCBI__GCF_000010525.1:WP_012172510.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O58478 Alanine/serine racemase; ASR; Ala/Ser racemase; EC 5.1.1.-; EC 5.1.1.1 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
31% identity, 94% coverage: 22:466/475 of query aligns to 31:455/474 of O58478

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D251 (≠ T247) mutation to A: Loss of activity.
K308 (= K304) mutation to A: Loss of activity.

1szkA The structure of gamma-aminobutyrate aminotransferase mutant: e211s (see paper)
31% identity, 89% coverage: 45:465/475 of query aligns to 23:416/425 of 1szkA

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active site: Y137 (= Y161), E205 (= E242), D238 (= D275), Q241 (= Q278), K267 (= K304), T296 (= T336), R397 (≠ T446)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G110 (= G133), S111 (= S134), Y137 (= Y161), H138 (= H162), E205 (= E242), D238 (= D275), V240 (≠ I277), Q241 (= Q278), K267 (= K304)

Sites not aligning to the query:

active site: 18

O50131 Ornithine aminotransferase; Orn-AT; Ornithine delta-aminotransferase; EC 2.6.1.13 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
32% identity, 90% coverage: 47:473/475 of query aligns to 39:446/454 of O50131

query
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O50131

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V

A

V

I

T

R

L

L

A

I

P

P

S

P

L

L

L

I

I

I

T

S

K

E

S

E

E

E

I

A

D

K

Q

M

A

G

I

L

R

D

L

I

L

F

D

E

L

E

L

A

L

I

T

K

R

V

V

V

T

S

T92 (≠ S99) mutation to V: Slightly increases the specific activity. Increases KM for L-ornithine.
D93 (≠ Q100) mutation to L: Reduces the specific activity. Increases KM for L-ornithine.
G124 (= G133) binding pyridoxal 5'-phosphate
T125 (≠ S134) binding pyridoxal 5'-phosphate
Q267 (= Q278) binding pyridoxal 5'-phosphate
K293 (= K304) modified: N6-(pyridoxal phosphate)lysine
T321 (= T336) binding pyridoxal 5'-phosphate

7vo1A Structure of aminotransferase-substrate complex (see paper)
32% identity, 90% coverage: 47:473/475 of query aligns to 37:444/452 of 7vo1A

query
sites
7vo1A

I

V

F

I

D

E

R

R

C

A

Q

E

G

G

S

V

Y

Y

M

W

Y

I

D

D

A

V

E

D

D

G

V

N

P

V

Y

L

L

L

D

D

L

F

Q

S

M

S

W

G

Y
x
I

S

G

A

V

V

M

N

N

F

V

G

G

Y

L

G

R

N

N

Q

P

R

K

L

V

N

I

K

E

A

A

L

I

T

K

R

K

Q

Q

L

L

D

D

R

L

L

V

P

L

Q

H

V

A

A

A

-

G

S

T

Q

D

Y

Y

L

Y

H

N

A

P

E

Y

K

Q

I

V

E

E

L

L

A

A

A

K

T

K

I

L

A

V

Q

E

H

I

A

A

E

P

K

G

T

D

W

I

G

E

A

R

G

-

R

K

V

V

H

F

F

L

N

S

V

N

G
x
S

G
|
G

S
x
T

Q

E

A

A

V

N

E

E

D

A

A

A

L

L

K

K

L

I

V

A

R

K

N

W

A

S

R

T

R

N

G

R

K

K

G

-

T

M

M

F

F

I

A

A

F

F

E

I

G

G

G

A

Y
x
F

H
|
H

G

G

R
|
R

T

T

L

H

G

G

A

T

S

M

A

S

I

L

T

T

S

A

S

S

-

K

-

P

Y

V

R

Q

Y

R

R

S

R

R

R

M

Y

F

G

P

H

T

F

M

D

P

R

G

A

V

L

V

F

H

V

V

P

P

F

Y

P

P

Y

N

H

P

F

Y

R

R

G

N

P

P

K

W

G

G

M

I

D

D

K

G

E

Y

E

E

Y

N

G

P

L

D

H

E

C

L

V

I

K

N

Q

R

F

V

E

I

R

D

L

Y

F

I

E

E

S

-

E

E

Y

Y

N

-

G

-

V

L

W

F

D

E

P

H

K

Y

A

V

G

P

E

A

A

E

E

E

C

V

A

A

A

G

F

I

F

F

V

F

E

E

P

P

I

I

Q

Q

G

G

T
x
E

G

G

Y

Y

V

V

I

V

P

P

H

K

N

N

F

F

I

K

E

E

L

L

K

K

V

L

L

A

D

D

R

K

H

H

G

G

I

I

L

L

V

I

V

D

D
|
D

E

E

I
x
V

Q
|
Q

M

M

G

G

V

M

Y

G

R

R

T

T

G

G

K

R

L

M

W

W

S

A

I

I

E

E

H

H

F

F

G

D

V

I

T

V

P

P

D

D

V

I

L

V

V

T

F

V

G

A

K
|
K

A

A

I

L

T

G

N

G

G

G

L

I

N

-

P

P

L

I

S

G

G

A

I

T

W

I

A

F

R

R

D

A

A

D

L

L

I

-

N

-

P

-

E

-

V

D

F

F

P

G

P

V

G

S

S

G

T

V

H

H

S

S

-
x
N

T
|
T

F

F

A

G

S

G

N

N

P

-

L

-

G

-

T

T

A

V

V

A

A

A

L

A

E

A

T

A

L

L

R

A

M

V

V

I

E

E

E

L

Q

Q

D

N

F

-

G

G

A

L

A

I

V

E

M

N

A

A

K

Q

G

K

-

L

-

E

A

P

Y

L

F

F

L

R

E

E

G

R

L

L

Q

E

Y

E

L

M

Q

K

R

E

S

K

H

Y

K

E

V

I

G

G

D

D

V

V

D

R

G

G

L

L

G

G

L

L

A

A

L

W

R

G

M

V

E

E

I

F

C

V

E

-

E

K

D

D

G

R

H

K

T

T

P

K

N

E

K

Y

A

A

L

T

V

K

D

E

R

R

M

-

V

-

D

-

I

-

A

-

L

-

A

-

A

G

D

E

L

I

E

V

L

V

D

E

G

A

R

L

R

K

H

R

G

G

L

L

V

A

L

L

D

L

I

-

G

-

G

G

Y

C

Y

G

K

K

N

S

V

A

V

I

T
x
R

L

L

A

I

P

P

S

P

L

L

L

I

I

I

T

S

K

E

S

E

E

E

I

A

D

K

Q

M

A

G

I

L

R

D

L

I

L

F

D

E

L

E

L

A

L

I

T

K

R

V

V

V

T

S

binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I61 (≠ Y71), S121 (≠ G132), G122 (= G133), T123 (≠ S134), F149 (≠ Y161), H150 (= H162), R152 (= R164), E234 (≠ T247), D262 (= D275), V264 (≠ I277), Q265 (= Q278), K291 (= K304), N318 (vs. gap), T319 (= T336), R417 (≠ T446)

7vntA Structure of aminotransferase-substrate complex (see paper)
32% identity, 90% coverage: 47:473/475 of query aligns to 37:444/452 of 7vntA

query
sites
7vntA

I

V

F

I

D

E

R

R

C

A

Q

E

G

G

S

V

Y

Y

M

W

Y

I

D

D

A

V

E

D

D

G

V

N

P

V

Y

L

L

L

D

D

L

F

Q

S

M

S

W

G

Y

I

S

G

A

V

V

M

N

N

F

V

G

G

Y

L

G

R

N

N

Q

P

R

K

L

V

N

I

K

E

A

A

L

I

T

K

R

K

Q

Q

L

L

D

D

R

L

L

V

P

L

Q

H

V

A

A

A

-

G

S

T

Q

D

Y

Y

L

Y

H

N

A

P

E

Y

K

Q

I

V

E

E

L

L

A

A

A

K

T

K

I

L

A

V

Q

E

H

I

A

A

E

P

K

G

T

D

W

I

G

E

A

R

G

-

R

K

V

V

H

F

F

L

N

S

V

N

G

S

G
|
G

S
x
T

Q

E

A

A

V

N

E

E

D

A

A

A

L

L

K

K

L

I

V

A

R

K

N

W

A

S

R

T

R

N

G

R

K

K

G

-

T

M

M

F

F

I

A

A

F

F

E

I

G

G

G

A

Y
x
F

H
|
H

G

G

R
|
R

T

T

L

H

G

G

A

T

S

M

A

S

I

L

T

T

S

A

S

S

-

K

-

P

Y

V

R

Q

Y

R

R

S

R

R

R

M

Y

F

G

P

H

T

F

M

D

P

R

G

A

V

L

V

F

H

V

V

P

P

F

Y

P

P

Y

N

H

P

F

Y

R

R

G

N

P

P

K

W

G

G

M

I

D

D

K

G

E

Y

E

E

Y

N

G

P

L

D

H

E

C

L

V

I

K

N

Q

R

F

V

E

I

R

D

L

Y

F

I

E

E

S

-

E

E

Y

Y

N

-

G

-

V

L

W

F

D

E

P

H

K

Y

A

V

G

P

E

A

A

E

E

E

C

V

A

A

A

G

F

I

F

F

V

F

E
|
E

P

P

I

I

Q

Q

G

G

T
x
E

G

G

Y

Y

V

V

I

V

P

P

H

K

N

N

F

F

I

K

E

E

L

L

K

K

V

L

L

A

D

D

R

K

H

H

G

G

I

I

L

L

V

I

V

D

D
|
D

E

E

I
x
V

Q
|
Q

M

M

G

G

V

M

Y

G

R

R

T

T

G

G

K

R

L

M

W

W

S

A

I

I

E

E

H

H

F

F

G

D

V

I

T

V

P

P

D

D

V

I

L

V

V

T

F

V

G

A

K
|
K

A

A

I

L

T

G

N

G

G

G

L

I

N

-

P

P

L

I

S

G

G

A

I

T

W

I

A

F

R

R

D

A

A

D

L

L

I

-

N

-

P

-

E

-

V

D

F

F

P

G

P

V

G

S

S

G

T

V

H

H

S

S

-

N

T

T

F

F

A

G

S

G

N

N

P

-

L

-

G

-

T

T

A

V

V

A

A

A

L

A

E

A

T

A

L

L

R

A

M

V

V

I

E

E

E

L

Q

Q

D

N

F

-

G

G

A

L

A

I

V

E

M

N

A

A

K

Q

G

K

-

L

-

E

A

P

Y

L

F

F

L

R

E

E

G

R

L

L

Q

E

Y

E

L

M

Q

K

R

E

S

K

H

Y

K

E

V

I

G

G

D

D

V

V

D

R

G

G

L

L

G

G

L

L

A

A

L

W

R

G

M

V

E

E

I

F

C

V

E

-

E

K

D

D

G

R

H

K

T

T

P

K

N

E

K

Y

A

A

L

T

V

K

D

E

R

R

M

-

V

-

D

-

I

-

A

-

L

-

A

-

A

G

D

E

L

I

E

V

L

V

D

E

G

A

R

L

R

K

H

R

G

G

L

L

V

A

L

L

D

L

I

-

G

-

G

G

Y

C

Y

G

K

K

N

S

V

A

V

I

T

R

L

L

A

I

P

P

S

P

L

L

L

I

I

I

T

S

K

E

S

E

E

E

I

A

D

K

Q

M

A

G

I

L

R

D

L

I

L

F

D

E

L

E

L

A

L

I

T

K

R

V

V

V

T

S

binding L-ornithine: F149 (≠ Y161), R152 (= R164), E234 (≠ T247), K291 (= K304)
binding pyridoxal-5'-phosphate: G122 (= G133), T123 (≠ S134), F149 (≠ Y161), H150 (= H162), E229 (= E242), D262 (= D275), V264 (≠ I277), Q265 (= Q278), K291 (= K304)

7vnoA Structure of aminotransferase (see paper)
32% identity, 90% coverage: 47:473/475 of query aligns to 37:444/452 of 7vnoA

query
sites
7vnoA

I

V

F

I

D

E

R

R

C

A

Q

E

G

G

S

V

Y

Y

M

W

Y

I

D

D

A

V

E

D

D

G

V

N

P

V

Y

L

L

L

D

D

L

F

Q

S

M

S

W

G

Y

I

S

G

A

V

V

M

N

N

F

V

G

G

Y

L

G

R

N

N

Q

P

R

K

L

V

N

I

K

E

A

A

L

I

T

K

R

K

Q

Q

L

L

D

D

R

L

L

V

P

L

Q

H

V

A

A

A

-

G

S

T

Q

D

Y

Y

L

Y

H

N

A

P

E

Y

K

Q

I

V

E

E

L

L

A

A

A

K

T

K

I

L

A

V

Q

E

H

I

A

A

E

P

K

G

T

D

W

I

G

E

A

R

G

-

R

K

V

V

H

F

F

L

N

S

V

N

G

S

G
|
G

S
x
T

Q

E

A

A

V
x
N

E

E

D

A

A

A

L

L

K

K

L

I

V

A

R

K

N

W

A

S

R

T

R

N

G

R

K

K

G

-

T

M

M

F

F

I

A

A

F

F

E

I

G

G

G

A

Y
x
F

H
|
H

G

G

R

R

T

T

L

H

G

G

A

T

S

M

A

S

I

L

T

T

S

A

S

S

-

K

-

P

Y

V

R

Q

Y

R

R

S

R

R

R

M

Y

F

G

P

H

T

F

M

D

P

R

G

A

V

L

V

F

H

V

V

P

P

F

Y

P

P

Y

N

H

P

F

Y

R

R

G

N

P

P

K

W

G

G

M

I

D

D

K

G

E

Y

E

E

Y

N

G

P

L

D

H

E

C

L

V

I

K

N

Q

R

F

V

E

I

R

D

L

Y

F

I

E

E

S

-

E

E

Y

Y

N

-

G

-

V

L

W

F

D

E

P

H

K

Y

A

V

G

P

E

A

A

E

E

E

C

V

A

A

A

G

F

I

F

F

V

F

E
|
E

P

P

I

I

Q

Q

G

G

T

E

G

G

Y

Y

V

V

I

V

P

P

H

K

N

N

F

F

I

K

E

E

L

L

K

K

V

L

L

A

D

D

R

K

H

H

G

G

I

I

L

L

V

I

V

D

D
|
D

E

E

I
x
V

Q
|
Q

M

M

G

G

V

M

Y

G

R

R

T

T

G

G

K

R

L

M

W

W

S

A

I

I

E

E

H

H

F

F

G

D

V

I

T

V

P

P

D

D

V

I

L

V

V

T

F

V

G

A

K
|
K

A

A

I

L

T

G

N

G

G

G

L

I

N

-

P

P

L

I

S

G

G

A

I

T

W

I

A

F

R

R

D

A

A

D

L

L

I

-

N

-

P

-

E

-

V

D

F

F

P

G

P

V

G

S

S

G

T

V

H

H

S

S

-

N

T

T

F

F

A

G

S

G

N

N

P

-

L

-

G

-

T

T

A

V

V

A

A

A

L

A

E

A

T

A

L

L

R

A

M

V

V

I

E

E

E

L

Q

Q

D

N

F

-

G

G

A

L

A

I

V

E

M

N

A

A

K

Q

G

K

-

L

-

E

A

P

Y

L

F

F

L

R

E

E

G

R

L

L

Q

E

Y

E

L

M

Q

K

R

E

S

K

H

Y

K

E

V

I

G

G

D

D

V

V

D

R

G

G

L

L

G

G

L

L

A

A

L

W

R

G

M

V

E

E

I

F

C

V

E

-

E

K

D

D

G

R

H

K

T

T

P

K

N

E

K

Y

A

A

L

T

V

K

D

E

R

R

M

-

V

-

D

-

I

-

A

-

L

-

A

-

A

G

D

E

L

I

E

V

L

V

D

E

G

A

R

L

R

K

H

R

G

G

L

L

V

A

L

L

D

L

I

-

G

-

G

G

Y

C

Y

G

K

K

N

S

V

A

V

I

T

R

L

L

A

I

P

P

S

P

L

L

L

I

I

I

T

S

K

E

S

E

E

E

I

A

D

K

Q

M

A

G

I

L

R

D

L

I

L

F

D

E

L

E

L

A

L

I

T

K

R

V

V

V

T

S

binding pyridoxal-5'-phosphate: G122 (= G133), T123 (≠ S134), N126 (≠ V137), F149 (≠ Y161), H150 (= H162), E229 (= E242), D262 (= D275), V264 (≠ I277), Q265 (= Q278), K291 (= K304)

1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
31% identity, 89% coverage: 45:465/475 of query aligns to 23:416/425 of 1sffA

query
sites
1sffA

P

P

K

I

I

F

F

A

D

D

R

R

C

A

Q

E

G

N

S

C

Y

R

M

V

Y

W

D

D

A

V

E

E

D

G

V

R

P

E

Y

Y

L

L

D

D

L

F

Q

A

M

G

W

G

Y

I

S

A

A

V

V

L

N

N

F

T

G

G

Y

H

G

L

N

H

Q

P

R

K

L

V

N

V

K

A

A

A

L

V

T

E

R

A

Q

Q

L

L

D

K

R

K

L

L

P

S

Q

H

V

T

A

C

S

F

Q
|
Q

Y

V

L

L

H

A

A

Y

E

E

K

P

-

Y

I

L

E

E

L

L

A

C

A

E

T

I

I

M

A

N

Q

Q

H

K

A

V

E

P

K

G

T

D

W

F

G

A

A

K

G

-

R

K

V

T

H

L

F

L

N

V

V

T

G

T

G
|
G

S
|
S

Q

E

A

A

V

V

E

E

D

N

A

A

L

V

K

K

L

I

V

A

R

R

N

A

A

A

R

T

R

K

G

R

K

S

G

G

T

T

M

I

F

-

A

A

F

F

E

S

G

G

G

A

Y
|
Y

H
|
H

G

G

R
|
R

T

T

L

H

G

Y

A

T

S

L

A

A

I

L

T

T

S

G

-

K

-

V

-
x
N

-

P

-

Y

S

S

Y

A

R

G

Y

M

R

G

R

L

R

M

Y

P

G

G

H

H

F

V

D

Y

R

R

A

A

L

L

F

Y

V

-

P

P

F

C

P

P

Y

L

H

H

F

-

R

-

G

-

P

-

K

-

G

G

M

I

D

S

K

E

E

D

Y

-

G

-

L

-

H

-

C

A

V

I

K

A

Q

S

F

I

E

H

R

R

L

I

F

F

E

K

S

N

E

D

Y

-

N

-

G

-

V

-

W

-

D

-

P

-

K

-

A

A

G

A

E

P

A

E

E

D

C

I

A

A

F

I

F

V

V

I

E
|
E

P

P

I

V

Q

Q

G

G

T

E

G

G

Y

F

V

Y

I

A

P

S

H

P

N

A

F

F

F

M

I

Q

E

R

L

L

K

R

K

A

V

L

L

C

D

D

R

E

H

H

G

G

I

I

L

M

L

L

V

I

V

A

D
|
D

E

E

I
x
V

Q
|
Q

M

S

G

G

V

A

Y

G

R

R

T

T

G

G

K

T

L

L

W

F

S

A

I

M

E

E

H

Q

F

M

G

G

V

V

T

A

P

P

D

D

V

L

L

T

V

T

F

F

G

A

K
|
K

A

S

I

I

T

A

N

G

G

G

L

F

N

-

P

P

L

L

S

A

G

G

I

V

W

T

A

G

R

R

D

A

A

E

L

V

I

M

N

D

P

-

E

-

V

A

F

V

P

A

P

P

G

G

S

G

T

L

H

G

S

G

T
|
T

F

Y

A

A

S

G

N

N

P

P

L

I

G

A

T

C

A

V

V

A

A

A

L

L

E

E

T

V

L

L

R

K

M

V

V

F

E

E

E

Q

E

E

Q

N

D

L

F

L

G

Q

A

K

A

A

V

-

M

N

A

D

K

L

G

G

A

Q

Y

K

F

L

L

K

E

D

G

G

L

L

Q

L

Y

A

L

I

Q

A

R

E

S

K

H

H

K

P

V

E

V

I

G

G

D

D

V

V

D

R

G

G

L

L

G

G

L

A

A

M

L

I

R

A

M

I

E

E

I

L

C

F

E

E

E

D

D

G

G

D

H

H

T

-

P

-

N

N

K

K

A

P

L

-

V

-

D

-

R

-

M

-

V

-

D

D

I

A

A

K

L

L

A

T

A

A

D

E

L

I

E

V

L

A

D

R

G

A

R

R

R

D

H

K

G

G

L

L

V

I

L

L

D

L

I

S

G

C

G

G

Y

P

Y

Y

K
x
Y

N

N

V

V

V

L

T
x
R

L

I

A

L

P

V

S

P

L

L

L

T

I

I

T

E

K

D

S

A

E

Q

I

I

D

R

Q

Q

A

G

I

L

R

E

L

I

active site: Y137 (= Y161), E205 (= E242), D238 (= D275), Q241 (= Q278), K267 (= K304), T296 (= T336), R397 (≠ T446)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Q78 (= Q100), G110 (= G133), S111 (= S134), Y137 (= Y161), H138 (= H162), R140 (= R164), E205 (= E242), D238 (= D275), V240 (≠ I277), Q241 (= Q278), K267 (= K304), T296 (= T336)
binding sulfate ion: N152 (vs. gap), Y393 (≠ K442)

Sites not aligning to the query:

active site: 18

1sf2A Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
31% identity, 89% coverage: 45:465/475 of query aligns to 23:416/425 of 1sf2A

query
sites
1sf2A

P

P

K

I

I

F

F

A

D

D

R

R

C

A

Q

E

G

N

S

C

Y

R

M

V

Y

W

D

D

A

V

E

E

D

G

V

R

P

E

Y

Y

L

L

D

D

L

F

Q

A

M

G

W

G

Y

I

S

A

A

V

V

L

N

N

F

T

G

G

Y

H

G

L

N

H

Q

P

R

K

L

V

N

V

K

A

A

A

L

V

T

E

R

A

Q

Q

L

L

D

K

R

K

L

L

P

S

Q

H

V

T

A

C

S

F

Q

Q

Y

V

L

L

H

A

A

Y

E

E

K

P

-

Y

I

L

E

E

L

L

A

C

A

E

T

I

I

M

A

N

Q

Q

H

K

A

V

E

P

K

G

T

D

W

F

G

A

A

K

G

-

R

K

V

T

H

L

F

L

N

V

V

T

G

T

G
|
G

S
|
S

Q

E

A

A

V

V

E

E

D

N

A

A

L

V

K

K

L

I

V

A

R

R

N

A

A

A

R

T

R

K

G

R

K

S

G

G

T

T

M

I

F

-

A

A

F

F

E

S

G

G

G

A

Y
|
Y

H
|
H

G

G

R

R

T

T

L

H

G

Y

A

T

S

L

A

A

I

L

T

T

S

G

-

K

-

V

-
x
N

-

P

-

Y

S

S

Y

A

R

G

Y

M

R

G

R

L

R

M

Y

P

G

G

H

H

F

V

D

Y

R

R

A

A

L

L

F

Y

V

-

P

P

F

C

P

P

Y

L

H

H

F

-

R

-

G

-

P

-

K

-

G

G

M

I

D

S

K

E

E

D

Y

-

G

-

L

-

H

-

C

A

V

I

K

A

Q

S

F

I

E

H

R

R

L

I

F

F

E

K

S

N

E

D

Y

-

N

-

G

-

V

-

W

-

D

-

P

-

K

-

A

A

G

A

E

P

A

E

E

D

C

I

A

A

F

I

F

V

V

I

E
|
E

P

P

I

V

Q

Q

G

G

T

E

G

G

Y

F

V

Y

I

A

P

S

H

P

N

A

F

F

F

M

I

Q

E

R

L

L

K

R

K

A

V

L

L

C

D

D

R

E

H

H

G

G

I

I

L

M

L

L

V

I

V

A

D
|
D

E

E

I
x
V

Q
|
Q

M

S

G

G

V

A

Y

G

R

R

T

T

G

G

K

T

L

L

W

F

S

A

I

M

E

E

H

Q

F

M

G

G

V

V

T

A

P

P

D

D

V

L

L

T

V

T

F

F

G

A

K
|
K

A

S

I

I

T

A

N

G

G

G

L

F

N

-

P

P

L

L

S

A

G

G

I

V

W

T

A

G

R

R

D

A

A

E

L

V

I

M

N

D

P

-

E

-

V

A

F

V

P

A

P

P

G

G

S

G

T

L

H

G

S

G

T
|
T

F

Y

A

A

S

G

N

N

P

P

L

I

G

A

T

C

A

V

V

A

A

A

L

L

E

E

T

V

L

L

R

K

M

V

V

F

E

E

E

Q

E

E

Q

N

D

L

F

L

G

Q

A

K

A

A

V

-

M

N

A

D

K

L

G

G

A

Q

Y

K

F

L

L

K

E

D

G

G

L

L

Q

L

Y

A

L

I

Q

A

R

E

S

K

H

H

K

P

V

E

V

I

G

G

D

D

V

V

D

R

G

G

L

L

G

G

L

A

A

M

L

I

R

A

M

I

E

E

I

L

C

F

E

E

E

D

D

G

G

D

H

H

T

-

P

-

N

N

K

K

A

P

L

-

V

-

D

-

R

-

M

-

V

-

D

D

I

A

A

K

L

L

A

T

A

A

D

E

L

I

E

V

L

A

D

R

G

A

R

R

R

D

H

K

G

G

L

L

V

I

L

L

D

L

I

S

G

C

G

G

Y

P

Y

Y

K
x
Y

N

N

V

V

V

L

T
x
R

L

I

A

L

P

V

S

P

L

L

L

T

I

I

T

E

K

D

S

A

E

Q

I

I

D

R

Q

Q

A

G

I

L

R

E

L

I

active site: Y137 (= Y161), E205 (= E242), D238 (= D275), Q241 (= Q278), K267 (= K304), T296 (= T336), R397 (≠ T446)
binding pyridoxal-5'-phosphate: G110 (= G133), S111 (= S134), Y137 (= Y161), H138 (= H162), E205 (= E242), D238 (= D275), V240 (≠ I277), Q241 (= Q278), K267 (= K304)
binding sulfate ion: N152 (vs. gap), Y393 (≠ K442)

Sites not aligning to the query:

active site: 18

P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
31% identity, 89% coverage: 45:465/475 of query aligns to 24:417/426 of P22256

query
sites
P22256

P

P

K

I

I

F

F

A

D

D

R

R

C

A

Q

E

G

N

S

C

Y

R

M

V

Y

W

D

D

A

V

E

E

D

G

V

R

P

E

Y

Y

L

L

D

D

L

F

Q

A

M

G

W

G

Y
x
I

S

A

A

V

V

L

N

N

F

T

G

G

Y

H

G

L

N

H

Q

P

R

K

L

V

N

V

K

A

A

A

L

V

T

E

R

A

Q

Q

L

L

D

K

R

K

L

L

P

S

Q

H

V

T

A

C

S

F

Q

Q

Y

V

L

L

H

A

A

Y

E

E

K

P

-

Y

I

L

E

E

L

L

A

C

A

E

T

I

I

M

A

N

Q

Q

H

K

A

V

E

P

K

G

T

D

W

F

G

A

A

K

G

-

R

K

V

T

H

L

F

L

N

V

V

T

G

T

G
|
G

S
|
S

Q

E

A

A

V

V

E

E

D

N

A

A

L

V

K

K

L

I

V

A

R

R

N

A

A

A

R

T

R

K

G

R

K

S

G

G

T

T

M

I

F

-

A

A

F

F

E

S

G

G

G

A

Y

Y

H

H

G

G

R

R

T

T

L

H

G

Y

A

T

S

L

A

A

I

L

T

T

S

G

-

K

-

V

-

N

-

P

-

Y

S

S

Y

A

R

G

Y

M

R

G

R

L

R

M

Y

P

G

G

H

H

F

V

D

Y

R

R

A

A

L

L

F

Y

V

-

P

P

F

C

P

P

Y

L

H

H

F

-

R

-

G

-

P

-

K

-

G

G

M

I

D

S

K

E

E

D

Y

-

G

-

L

-

H

-

C

A

V

I

K

A

Q

S

F

I

E

H

R

R

L

I

F

F

E

K

S

N

E

D

Y

-

N

-

G

-

V

-

W

-

D

-

P

-

K

-

A

A

G

A

E

P

A

E

E

D

C

I

A

A

F

I

F

V

V

I

E

E

P

P

I

V

Q

Q

G

G

T
x
E

G

G

Y

F

V

Y

I

A

P

S

H

P

N

A

F

F

F

M

I

Q

E

R

L

L

K

R

K

A

V

L

L

C

D

D

R

E

H

H

G

G

I

I

L

M

L

L

V

I

V

A

D

D

E

E

I
x
V

Q
|
Q

M

S

G

G

V

A

Y

G

R

R

T

T

G

G

K

T

L

L

W

F

S

A

I

M

E

E

H

Q

F

M

G

G

V

V

T

A

P

P

D

D

V

L

L

T

V

T

F

F

G

A

K
|
K

A

S

I

I

T

A

N

G

G

G

L

F

N

-

P

P

L

L

S

A

G

G

I

V

W

T

A

G

R

R

D

A

A

E

L

V

I

M

N

D

P

-

E

-

V

A

F

V

P

A

P

P

G

G

S

G

T

L

H

G

S

G

T
|
T

F

Y

A

A

S

G

N

N

P

P

L

I

G

A

T

C

A

V

V

A

A

A

L

L

E

E

T

V

L

L

R

K

M

V

V

F

E

E

E

Q

E

E

Q

N

D

L

F

L

G

Q

A

K

A

A

V

-

M

N

A

D

K

L

G

G

A

Q

Y

K

F

L

L

K

E

D

G

G

L

L

Q

L

Y

A

L

I

Q

A

R

E

S

K

H

H

K

P

V

E

V

I

G

G

D

D

V

V

D

R

G

G

L

L

G

G

L

A

A

M

L

I

R

A

M

I

E

E

I

L

C

F

E

E

E

D

D

G

G

D

H

H

T

-

P

-

N

N

K

K

A

P

L

-

V

-

D

-

R

-

M

-

V

-

D

D

I

A

A

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L

L

A

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A

A

D

E

L

I

E

V

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A

D

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A

R

R

R

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H

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G

G

L

L

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I

L

L

D

L

I

S

G

C

G

G

Y

P

Y

Y

K

Y

N

N

V

V

V

L

T

R

L

I

A

L

P

V

S

P

L

L

L

T

I

I

T

E

K

D

S

A

E

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I

I

D

R

Q

Q

A

G

I

L

R

E

L

I

I50 (≠ Y71) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
GS 111:112 (= GS 133:134) binding pyridoxal 5'-phosphate
E211 (≠ T247) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
V241 (≠ I277) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
Q242 (= Q278) binding pyridoxal 5'-phosphate
K268 (= K304) modified: N6-(pyridoxal phosphate)lysine
T297 (= T336) binding pyridoxal 5'-phosphate

2eo5A Crystal structure of 4-aminobutyrate aminotransferase from sulfolobus tokodaii strain7
31% identity, 90% coverage: 45:472/475 of query aligns to 25:412/412 of 2eo5A

query
sites
2eo5A

P

P

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Y

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N

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x
T

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A

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N

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x
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A

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-

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T

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x
R

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Q

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active site: F139 (≠ Y161), E219 (= E242), D252 (= D275), Q255 (= Q278), K281 (= K304), T303 (= T336), R386 (≠ T446)
binding pyridoxal-5'-phosphate: G113 (= G133), T114 (≠ S134), F139 (≠ Y161), H140 (= H162), E219 (= E242), D252 (= D275), V254 (≠ I277), Q255 (= Q278), K281 (= K304)

Sites not aligning to the query:

active site: 18

4ysnC Structure of aminoacid racemase in complex with plp (see paper)
28% identity, 96% coverage: 20:475/475 of query aligns to 11:444/448 of 4ysnC

query
sites
4ysnC

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D

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A

D

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A

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A

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Y

I

Y

A

A

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F

A

A

A

-

D

-

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-

L

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H

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G

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V

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-

I

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I

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A

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G

Y

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K

-

N

N

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x
R

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F

A

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P

L

L

L

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I

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E

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A

A

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L

V

L

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D

L

D

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T

R

A

V

V

T

E

R

N

G

G

active site: A29 (≠ D38), Y149 (= Y161), E224 (= E242), D257 (= D275), N260 (≠ Q278), K287 (= K304), T316 (≠ S335), R415 (≠ T446)
binding pyridoxal-5'-phosphate: S121 (≠ G132), G122 (= G133), S123 (= S134), Y149 (= Y161), H150 (= H162), E224 (= E242), D257 (= D275), V259 (≠ I277), K287 (= K304), F315 (≠ H334), T316 (≠ S335)

6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
31% identity, 90% coverage: 45:472/475 of query aligns to 31:446/448 of 6io1B

query
sites
6io1B

P

P

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Q

F

F

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G

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A

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N

E

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A

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H

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K

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I

V

W

G

A

V

R

S

D

D

A

E

L

I

I

A

N

E

P

T

-

L

-

A

-

S

-

A

-

D

E

R

V

V

F

F

P

M

P

H

G

G

S

F

T

T

H

Y

S

S

T

-

F

-

A

-

S

G

N

H

P

P

L

V

G

A

T

C

A

A

V

V

A

A

L

L

E

R

T

N

L

L

R

D

M

I

V

L

E

L

E

A

E

E

Q

R

D

L

F

W

G

E

A

N

A

A

V

A

M

-

A

A

K

S

G

G

A

A

Y

Y

F

L

L

L

E

Q

G

E

L

L

Q

K

Y

R

L

L

Q

E

R

-

S

E

H

R

K

P

V

Y

V

V

G

G

D

E

V

V

D

R

G

G

L

K

G

G

L

L

A

M

L

L

R

L

M

V

E

E

I

V

C

V

E

R

E

-

D

-

G

-

H

-

T

-

P

-

N

D

K

K

A

A

L

S

V

K

D

E

R

K

M

F

-

P

V

P

D

E

I

F

A

K

L

L

A

G

A

P

D

K

L

L

E

E

L

A

D

A

G

T

R

R

H

R

G

G

L

I

V

I

L

V

D

R

I

C

G

-

Y

-

Y

T

K

P

N

D

V

G

V

I

T

V

L

M

A

A

P

P

S

P

L

L

L

T

I

I

T

S

K

R

S

A

E

E

I

C

D

D

Q

V

A

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I

I

R

E

L

G

L

V

D

A

L

A

L

L

T

S

R

D

V

V

active site: Y151 (= Y161), D257 (= D275), K286 (= K304)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G132), G118 (= G133), A119 (≠ S134), N122 (≠ V137), Y151 (= Y161), H152 (= H162), D257 (= D275), V259 (≠ I277), I260 (≠ Q278), K286 (= K304)

Sites not aligning to the query:

active site: 20

5wyaA Structure of amino acid racemase, 2.65 a (see paper)
28% identity, 96% coverage: 20:475/475 of query aligns to 2:435/439 of 5wyaA

query
sites
5wyaA

V

L

S

D

R

K

E

A

T

S

R

K

L

L

L

I

A

D

L

E

E

E

Q

N

R

K

Y

Y

C

Y

S

A

H

R

G

S

D
x
A

T

R

V

I

H

N

Y

Y

S

Y

E

N

P

-

K

L

I

V

F

I

D

D

R

H

C

A

Q

H

G

G

S

A

Y

T

M

L

Y

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A

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E

D

D

G

V

N

P

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Y

Y

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I

D

D

L

L

Q

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M

A

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Y
x
A

S

S

A

A

V

I

N

N

F

V

G

G

Y

H

G

T

N

H

Q

E

R

K

L

V

N

V

K

K

A

A

L

I

T

A

R

D

Q

Q

L

A

D

Q

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K

L

L

P

I

Q

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V

Y

A

T

S

P

Q

A

Y
|
Y

L

F

H

H

-

H

A

V

E

P

K

G

I

M

E

E

L

L

A

S

A

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T

K

I

L

A

A

Q

K

H

I

A

A

E

-

K

-

T

P

W

G

G

N

A

S

G

P

G

K

R

M

V

V

H

S

F

F

N

G

V

N

G
x
S

G
|
G

S
|
S

Q

D

A

A

V

N

E

D

D

A

A

I

L

I

K

K

L

F

V

A

R

R

N

-

A

A

R

Y

R

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G

K

R

G

Q

T

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M

I

F

V

A

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F

Y

E

M

G

G

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Y
|
Y

H
|
H

G

G

R

S

T

T

L

Y

G

G

A

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Q

A

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I

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-

G

S

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L

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M

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-

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F

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A

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F

Y

P

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P

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P

G

G

M

E

D

T

K

E

E

H

E

D

Y

V

G

S

L

L

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V

F

K

N

Q

E

F

F

E

K

R

K

L

P

F

F

E

E

S

S

E

-

Y

-

N

-

G

-

V

-

W

F

D

L

P

P

K

-

A

A

G

D

E

E

A

T

E

A

C

C

A

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A

-

F

-

F

L

V

I

E
|
E

P

P

I

I

Q

Q

G

G

T

D

G

G

Y

I

V

I

I

K

P

A

P

P

H

E

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F

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Y

K

K

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F

L

C

D

H

R

E

H

H

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G

I

I

L

L

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F

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A

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D

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I
x
V

Q
x
N

M

Q

G

G

V

L

Y

G

R

R

T

T

G

G

K

K

L

M

W

W

S

A

I

I

E

Q

H

Q

F

F

-

K

G

D

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E

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P

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D

V

L

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M

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S

F

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G

G

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|
K

A

S

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A

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S

G

G

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M

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-

P

P

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I

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W

I

A

G

R

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D

K

A

E

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I

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-

E

S

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L

F

D

P

A

P

P

G

A

S

H

T

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H
x
F

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x
T

T

T

F

-

A

A

S

G

N

N

P

P

L

V

G

C

T

S

A

A

V

A

A

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L

L

E

A

T

T

L

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M

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A

A

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G

G

A

A

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M

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G

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D

V

V

D

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M

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W

G

G

L

L

A

N

L

G

R

G

M

I

E

E

I

L

C

V

E

K

E

D

-

P

D

K

G

T

H

K

T

E

P

P

N

D

K

S

A

D

L

A

V

A

D

T

R

K

M

V

V

I

D

Y

I

Y

A

A

L

F

A

A

A

-

D

-

L

-

E

-

L

-

D

-

G

-

R

-

H

H

G

G

L

V

V

V

-

I

L

I

D

T

I

L

G

A

G

G

Y

-

K

-

N

N

V

I

V

L

T
x
R

L

F

A

Q

P

P

S

P

L

L

L

V

I

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T

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K

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E

E

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A

A

I

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D

L

D

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A

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F

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T

R

A

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G

G

active site: A20 (≠ D38), Y140 (= Y161), E215 (= E242), D248 (= D275), N251 (≠ Q278), K278 (= K304), T307 (≠ S335), R406 (≠ T446)
binding (2S,3S)-3-methyl-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]pentanoic acid: A52 (≠ Y71), Y82 (= Y101), S112 (≠ G132), G113 (= G133), S114 (= S134), Y140 (= Y161), H141 (= H162), E215 (= E242), D248 (= D275), V250 (≠ I277), N251 (≠ Q278), K278 (= K304), F306 (≠ H334), T307 (≠ S335), R406 (≠ T446)

5wyfA Structure of amino acid racemase, 2.12 a (see paper)
28% identity, 96% coverage: 20:475/475 of query aligns to 4:437/446 of 5wyfA

query
sites
5wyfA

V

L

S

D

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K

E

A

T

S

R

K

L

L

L

I

A

D

L

E

E

E

Q

N

R

K

Y

Y

C

Y

S

A

H

R

G

S

D
x
A

T

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V

I

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N

Y

Y

S

Y

E

N

P

-

K

L

I

V

F

I

D

D

R

H

C

A

Q

H

G

G

S

A

Y

T

M

L

Y

V

D

D

A

V

E

D

D

G

V

N

P

K

Y

Y

L

I

D

D

L

L

Q

L

M

A

W

S

Y
x
A

S

S

A

A

V

I

N

N

F

V

G

G

Y

H

G

T

N

H

Q

E

R

K

L

V

N

V

K

K

A

A

L

I

T

A

R

D

Q

Q

L

A

D

Q

R

K

L

L

P

I

Q

H

V

Y

A

T

S

P

Q

A

Y
|
Y

L

F

H

H

-

H

A

V

E

P

K

G

I

M

E

E

L

L

A

S

A

E

T

K

I

L

A

A

Q

K

H

I

A

A

E

P

K

G

T

N

W

-

G

-

A

S

G

P

G

K

R

M

V

V

H

S

F

F

N

G

V

N

G

S

G
|
G

S
|
S

Q

D

A

A

V

N

E

D

D

A

A

I

L

I

K

K

L

F

V

A

R

R

N

-

A

A

R

Y

R

T

G

G

K

R

G

Q

T

Y

M

I

F

V

A

S

F

Y

E

M

G

G

G

S

Y
|
Y

H
|
H

G

G

R

S

T

T

L

Y

G

G

A

S

S

Q

A

T

I

L

T

S

-

G

S

S

Y

L

R

N

Y

M

R

T

R

R

R

K

Y

I

G

G

-

P

H

M

F

L

D

P

R

S

A

V

L

V

F

H

V

V

P

P

F

Y

P

P

Y

D

H

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F

Y

R

R

G

T

P

Y

K

P

G

G

M

E

D

T

K

E

E

H

E

D

Y

V

G

S

L

L

H

R

C

Y

V

F

K

N

Q

E

F

F

E

K

R

K

L

P

F

F

E

E

S

S

E

-

Y

-

N

-

G

-

V

-

W

F

D

L

P

P

K

-

A

A

G

D

E

E

A

T

E

A

C

C

A

V

A

-

F

-

F

L

V

I

E
|
E

P

P

I

I

Q

Q

G

G

T
x
D

G

G

Y

I

V

I

I

K

P

A

P

P

H

E

N

E

F

Y

F

M

I

Q

E

L

L

V

K

Y

K

K

V

F

L

C

D

H

R

E

H

H

G

G

I

I

L

L

L

F

V

A

V

I

D
|
D

E

E

I
x
V

Q
x
N

M

Q

G

G

V

L

Y

G

R

R

T

T

G

G

K

K

L

M

W

W

S

A

I

I

E

Q

H

Q

F

F

-

K

G

D

V

I

T

E

P

P

D

D

V

L

L

M

V

S

F

V

G

G

K
|
K

A

S

I

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T

A

N

S

G

G

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M

N

-

P

P

L

L

S

S

G

A

I

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I

A

G

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D

K

A

E

L

V

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N

Q

P

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E

S

V

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D

P

A

P

P

G

A

S

H

T

L

H
x
F

S
x
T

T

T

F

-

A

A

S

G

N

N

P

P

L

V

G

C

T

S

A

A

V

A

A

S

L

L

E

A

T

T

L

L

R

D

M

V

V

I

E

E

E

Y

E

E

Q

G

D

L

F

V

G

E

A

K

A

S

V

A

M

-

A

T

K

D

G

G

A

A

Y

Y

F

A

L

K

E

Q

G

R

L

F

Q

L

Y

E

L

M

Q

Q

R

Q

S

R

H

H

K

P

V

M

V

I

G

G

D

D

V

V

D

R

G

M

L

W

G

G

L

L

A

N

L

G

R

G

M

I

E

E

I

L

C

V

E

K

E

D

-

P

D

K

G

T

H

K

T

E

P

P

N

D

K

S

A

D

L

A

V

A

D

T

R

K

M

V

V

I

D

Y

I

Y

A

A

L

F

A

A

A

-

D

-

L

-

E

-

L

-

D

-

G

-

R

-

H

H

G

G

L

V

V

V

-

I

L

I

D

T

I

L

G

A

G

G

Y

-

K

-

N

N

V

I

V

L

T
x
R

L

F

A

Q

P

P

S

P

L

L

L

V

I

I

T

P

K

R

S

E

E

Q

I

L

D

D

Q

Q

A

A

I

L

R

Q

L

V

L

L

D

D

L

D

L

A

L

F

T

T

R

A

V

V

T

E

R

N

G

G

active site: A22 (≠ D38), Y142 (= Y161), E217 (= E242), D250 (= D275), N253 (≠ Q278), K280 (= K304), T309 (≠ S335), R408 (≠ T446)
binding n-[o-phosphono-pyridoxyl]-isoleucine: A54 (≠ Y71), Y84 (= Y101), G115 (= G133), S116 (= S134), Y142 (= Y161), H143 (= H162), D222 (≠ T247), D250 (= D275), V252 (≠ I277), N253 (≠ Q278), K280 (= K304), F308 (≠ H334), T309 (≠ S335), R408 (≠ T446)

6s54A Transaminase from pseudomonas fluorescens (see paper)
28% identity, 90% coverage: 39:466/475 of query aligns to 25:445/453 of 6s54A

query
sites
6s54A

T

A

V

L

H

H

Y

E

S

Q

E

R

P

P

K

L

I

I

F

I

D

Q

R

S

C

G

Q

K

G

G

S

V

Y

H

M

I

Y

T

D

D

A

V

E

D

D

G

V

R

P

R

Y

F

L

I

D

D

L

C

Q

Q

M

G

W

G

Y

L

S

W

A

C

V

V

N

N

F

A

G

G

Y

Y

G

G

N

R

Q

R

R

E

L

I

N

I

K

D

A

A

L

V

T

T

R

R

Q

Q

L

M

D

E

R

E

L

L

P

A

-

Y

Q

S

V

L

A

F

S

P

Q

G

Y

S

L

T

H

N

A

A

E

P

K

A

I

I

E

A

L

L

A

S

A

Q

T

K

I

L

A

T

Q

E

H

V

A

A

E

A

K

E

T

E

W

-

G

-

A

G

G

M

G

V

R

K

V

A

H

S

F

F

N

G

V

L

G

G

G
|
G

S
|
S

Q

D

A

A

V

V

E

E

D

T

A

A

L

L

K

K

L

I

V

A

R

R

N

Q

A

Y

R

W

R

K

G

L

K

E

G

G

-

Q

-

P

-

D

-

K

-

V

T

K

M

F

F

V

A

S

F

L

E

Y

G

N

G

G

Y
|
Y

H
|
H

G
|
G

R

L

T

N

L

F

G

G

A

G

S

M

A

S

I

A

T

C

S

G

Y

N

R

A

Y

W

R

K

R

S

Y

Y

G

E

H

P

F

L

D

M

R

P

A

G

L

F

F

F

-

Q

V

V

P

E

F

S

P

P

Y

H

H

L

F

Y

R

R

G

N

P

P

K

F

G

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M

N

D

D

K

P

E

E

Y

L

G

A

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E

H

I

C

C

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A

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Q

Q

I

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E

E

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R

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A

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-

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-

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-

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A

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E

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P

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I

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x
V

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x
I

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G

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Y

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P

P

L

L

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S

G

A

I

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-

L

-

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-

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-

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-

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-

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-

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-

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-

A

W

W

A

E

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R

D

D

A

A

L

G

I

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-

E

S

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V

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Y

P

M

P

H

G

G

S

Y

T

T

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Y

S

S

T

-

F

-

A

-

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G

N

H

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P

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C

A

A

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A

A

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L

E

A

T

A

L

I

R

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M

I

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E

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E

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-

D

N

F

L

G

A

A

E

A

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A

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A

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V

G

G

A

D

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Y

F

F

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E

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I

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A

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G

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D

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V

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G

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K

G

G

L

L

A

M

L

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A

M

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E

E

I

L

C

V

E

K

E

E

D

R

G

A

H

T

T

K

P

E

N

P

K

F

A

G

L

P

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A

D

D

R

A

M

Y

V

-

D

P

I

L

A

A

L

I

A

S

A

E

D

A

L

C

E

V

L

N

D

N

G

G

R

V

R

M

H

I

G

R

L

T

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I

L

V

D

-

I

-

G

-

Y

-

K

-

N

N

V

K

V

L

T

I

L

I

A

S

P

P

S

P

L

L

L

T

I

F

T

T

K

T

S

E

E

H

I

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D

D

Q

E

A

V

I

I

R

E

L

V

L

L

D

D

active site: Y152 (= Y161), D258 (= D275), K287 (= K304)
binding pyridoxal-5'-phosphate: G119 (= G133), S120 (= S134), Y152 (= Y161), H153 (= H162), G154 (= G163), E225 (= E242), D258 (= D275), V260 (≠ I277), I261 (≠ Q278), K287 (= K304)

Sites not aligning to the query:

active site: 20

P73133 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
31% identity, 89% coverage: 45:465/475 of query aligns to 43:421/429 of P73133

query
sites
P73133

P

P

K

I

I

A

F

I

D

A

R

R

C

G

Q

Q

G

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A

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Y

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D

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A

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A

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Q

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Y

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G

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L

A

A

A

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I

A

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E

H

H

A

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-

K

-

T

-

W

-

G

-

A

C

G

A

G

D

R

R

V

V

H

F

F

F

N

C

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N

G
x
S

G
|
G

S
x
A

Q

E

A

A

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N

E

E

D

A

A

A

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I

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K

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L

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R

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-

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|
R

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T

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A

A

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I

A

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I

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Y

Y

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R

-

Y

-

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Y

F

F

D

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A

-

L

-

F

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V

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P

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-

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-

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-

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-

G

G

M

F

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D

K

Y

E

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Y

Y

G

-

L

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C

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F

L

E

E

R

-

L

-

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-

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-

N

N

G

K

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W

A

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A

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A

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x
E

G

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Y

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A

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G

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Y

-

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P

N

K

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x
R

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L

S125 (≠ G132) mutation to A: Retains 14% of N-acetylornithine aminotransferase wild-type activity.
G126 (= G133) mutation to A: Loss of N-acetylornithine aminotransferase activity.
A127 (≠ S134) mutation to S: Retains 9.1% of N-acetylornithine aminotransferase wild-type activity.
R163 (= R164) mutation to A: 4100-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 9.5% of N-acetylornithine aminotransferase wild-type activity.
E223 (≠ T247) mutation to A: 65-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 50% of N-acetylornithine aminotransferase wild-type activity.; mutation to S: 73-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Shows 160% of N-acetylornithine aminotransferase wild-type activity.
D251 (= D275) mutation to A: Loss of N-acetylornithine aminotransferase activity.; mutation to E: Retains 20% of N-acetylornithine aminotransferase wild-type activity.
Q254 (= Q278) mutation to A: Retains 0.9% of N-acetylornithine aminotransferase wild-type activity.
K280 (= K304) mutation to A: Loss of N-acetylornithine aminotransferase activity.
T308 (= T336) mutation to A: Retains 0.3% of N-acetylornithine aminotransferase wild-type activity.
R402 (≠ T446) mutation to A: 2080-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 18% of N-acetylornithine aminotransferase wild-type activity.

Sites not aligning to the query:

39 Y→F: Retains 7.4% of N-acetylornithine aminotransferase wild-type activity.

5kr6B Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
27% identity, 88% coverage: 46:464/475 of query aligns to 35:448/460 of 5kr6B

query
sites
5kr6B

K

R

I

I

F

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A

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A

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-

H

-

A

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-

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W

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A

A

P

G

A

G

G

-

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V

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Y

N

G

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M

G
x
S

G
|
G

S
|
S

Q

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A

A

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E

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A

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K

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A

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A

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A

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-

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A

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E

K

D

D

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K

H

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-

R

-

F

-

D

P

P

N

S

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A

K

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A

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A

A

C

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L

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E

A

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G

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E

I

L

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G

A

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H

H

G

G

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-

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-

L

-

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-

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G

-

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-

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-

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D

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L

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A

A

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I

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E

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D

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I

binding pyridoxal-5'-phosphate: S121 (≠ G132), G122 (= G133), S123 (= S134), Y155 (= Y161), H156 (= H162), G157 (= G163), E228 (= E242), D261 (= D275), V263 (≠ I277), K290 (= K304)

5lhaA Amine transaminase crystal structure from an uncultivated pseudomonas species in the pmp-bound form
30% identity, 91% coverage: 41:472/475 of query aligns to 21:446/447 of 5lhaA

query
sites
5lhaA

H

H

Y

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S

D

E

K

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T

K

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F

I

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A

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D

D

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R

Y

M

L

L

D

D

L

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Q

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M

G

W

G

Y

L

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W

A

N

V

V

N

N

F

I

G

G

Y

H

G

N

N

R

Q

A

R

S

L

V

N

K

K

A

A

A

L

I

T

A

R

A

Q

Q

L

L

D

D

R

E

L

L

P

A

-

Y

Q

Q

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A

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D

Q

G

Y

I

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A

H

H

A

P

E

R

K

V

I

F

E

D

L

L

A

A

A

E

T

R

I

L

A

T

Q

G

H

M

A

F

E

A

K

Q

T

E

W

R

G

M

A

A

G

-

R

R

V

V

H

L

F

F

N

S

V

S

G

G

G
|
G

S
|
S

Q

D

A

A

V

V

E

E

D

T

A

A

L

L

K

K

L

M

V

A

R

R

-

Q

-

Y

-

W

-

I

-

A

N

S

A

G

R

E

R

P

G

G

K

R

G

T

T

R

M

F

F

L

A

S

F

L

E

R

G

N

G

G

Y
|
Y

H
|
H

G
|
G

R

V

T

H

L

M

G

G

A

G

S

T

A

S

I

V

T

G

S

G

S

N

Y

G

R

V

Y

Y

R

H

R

Y

R

N

Y

H

G

G

H

Q

F

L

D

L

R

A

A

G

L

C

-

H

F

L

V

L

P

D

F

T

P

P

Y

W

H

L

F

Y

R

R

G

N

P

P

-

W

K

D

G

C

M

R

D

D

K

P

E

Q

E

A

Y

L

G

T

L

A

H

H

C

C

V

I

K

R

Q

Q

F

L

E

E

R

E

L

Q

F

I

E

A

S

-

E

-

Y

-

N

-

G

-

V

-

W

-

D

-

P

-

K

L

A

L

G

G

E

A

A

Q

E

T

C

I

A

A

F

L

F

I

V

A

E
|
E

P

P

I

V

Q

Q

G

G

T

A

G

G

Y

V

V

I

I

V

P

P

H

A

N

D

F

Y

F

W

I

P

E

R

L

L

K

R

K

E

V

V

L

C

D

D

R

R

H

H

G

G

I

I

L

L

V

I

V

A

D
|
D

E

E

I

V

Q

V

M

T

G

G

V

F

Y

G

R

R

T

S

G

G

K

C

L

M

W

L

S

G

I

S

E

R

H

G

F

W

G

G

V

V

T

A

P

P

D

D

V

I

L

L

V

C

F

L

G

A

K
|
K

A

G

I

I

T

T

N

A

G

G

L

Y

N

I

P

P

L

L

S

G

G

A

I

T

W

L

-

F

-

N

-

Q

-

R

A

I

R

A

D

D

A

A

L

I

I

E

N

N

P

G

E

Q

V

G

F

F

P

S

P

H

G

M

S

I

T

M

H

H

S

G

-
x
Y

T
|
T

F

Y

A

S

S

G

N

H

P

P

L

T

G

A

T

C

A

A

V

A

A

A

L

L

E

A

T

V

L

L

R

D

M

I

V

V

E

E

E

A

E

E

Q

-

D

D

F

L

G

P

A

G

A

N

V

A

M

A

A

K

K

V

G

G

A

A

Y

Q

F

L

L

L

E

E

G

Q

L

L

Q

Q

Y

P

L

L

Q

V

R

E

S

R

H

Y

K

A

V

V

G

G

D

E

V

V

D

R

G

G

L

K

G

G

L

L

A

M

L

I

R

A

M

L

E

D

I

L

C

V

E

-

E

S

D

D

G

K

H

R

T

T

-

R

-

Q

-

P

-

L

-

D

P

P

N

A

K

A

A

G

L

Q

V

P

D

S

R

R

M

I

V

A

D

D

I

-

A

-

L

-

A

-

D

-

L

-

E

-

L

-

D

E

G

A

R

R

H

A

G

G

-

V

L

L

V

V

L

R

D

P

I

I

G

G

G

-

Y

-

K

-

N

N

V

K

V

I

T

I

L

L

A

S

P

P

S

P

L

L

L

T

I

L

T

T

K

R

S

D

E

E

I

A

D

G

Q

L

A

M

I

V

R

S

L

A

L

L

D

E

L

A

L

F

T

A

R

R

V

C

active site: Y146 (= Y161), D253 (= D275), K282 (= K304), T319 (= T336)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G113 (= G133), S114 (= S134), Y146 (= Y161), H147 (= H162), G148 (= G163), E220 (= E242), D253 (= D275), K282 (= K304), Y318 (vs. gap), T319 (= T336)

5lh9D Amine transaminase crystal structure from an uncultivated pseudomonas species in the plp-bound (internal aldimine) form
30% identity, 91% coverage: 41:472/475 of query aligns to 23:448/449 of 5lh9D

query
sites
5lh9D

H

H

Y

R

S

D

E

K

P

T

K

L

I

V

F

I

D

A

R

R

C

G

Q

D

G

G

S

N

Y

Y

M

I

Y

T

D

D

A

I

E

D

D

G

V

Q

P

R

Y

M

L

L

D

D

L

G

Q

V

M

G

W

G

Y

L

S

W

A

N

V

V

N

N

F

I

G

G

Y

H

G

N

N

R

Q

A

R

S

L

V

N

K

K

A

A

A

L

I

T

A

R

A

Q

Q

L

L

D

D

R

E

L

L

P

A

-

Y

Q

Q

V

T

A

F

S

D

Q

G

Y

I

L

A

H

H

A

P

E

R

K

V

I

F

E

D

L

L

A

A

A

E

T

R

I

L

A

T

Q

G

H

M

A

F

E

A

K

Q

T

E

W

R

G

M

A

A

G

-

R

R

V

V

H

L

F

F

N

S

V

S

G

G

G
|
G

S
|
S

Q

D

A

A

V

V

E

E

D

T

A

A

L

L

K

K

L

M

V

A

R

R

-

Q

-

Y

-

W

-

I

-

A

N

S

A

G

R

E

R

P

G

G

K

R

G

T

T

R

M

F

F

L

A

S

F

L

E

R

G

N

G

G

Y
|
Y

H
|
H

G
|
G

R

V

T

H

L

M

G

G

A

G

S

T

A

S

I

V

T

G

S

G

S

N

Y

G

R

V

Y

Y

R

H

R

Y

R

N

Y

H

G

G

H

Q

F

L

D

L

R

A

A

G

L

C

-

H

F

L

V

L

P

D

F

T

P

P

Y

W

H

L

F

Y

R

R

G

N

P

P

-

W

K

D

G

C

M

R

D

D

K

P

E

Q

E

A

Y

L

G

T

L

A

H

H

C

C

V

I

K

R

Q

Q

F

L

E

E

R

E

L

Q

F

I

E

A

S

-

E

-

Y

-

N

-

G

-

V

-

W

-

D

-

P

-

K

L

A

L

G

G

E

A

A

Q

E

T

C

I

A

A

F

L

F

I

V

A

E
|
E

P

P

I

V

Q

Q

G

G

T

A

G

G

Y

V

V

I

I

V

P

P

H

A

N

D

F

Y

F

W

I

P

E

R

L

L

K

R

K

E

V

V

L

C

D

D

R

R

H

H

G

G

I

I

L

L

V

I

V

A

D
|
D

E

E

I
x
V

Q

V

M

T

G

G

V

F

Y

G

R

R

T

S

G

G

K

C

L

M

W

L

S

G

I

S

E

R

H

G

F

W

G

G

V

V

T

A

P

P

D

D

V

I

L

L

V

C

F

L

G

A

K
|
K

A

G

I

I

T

T

N

A

G

G

L

Y

N

I

P

P

L

L

S

G

G

A

I

T

W

L

-

F

-

N

-

Q

-

R

A

I

R

A

D

D

A

A

L

I

I

E

N

N

P

G

E

Q

V

G

F

F

P

S

P

H

G

M

S

I

T

M

H

H

S

G

-

Y

T
|
T

F

Y

A

S

S

G

N

H

P

P

L

T

G

A

T

C

A

A

V

A

A

A

L

L

E

A

T

V

L

L

R

D

M

I

V

V

E

E

E

A

E

E

Q

-

D

D

F

L

G

P

A

G

A

N

V

A

M

A

A

K

K

V

G

G

A

A

Y

Q

F

L

L

L

E

E

G

Q

L

L

Q

Q

Y

P

L

L

Q

V

R

E

S

R

H

Y

K

A

V

V

G

G

D

E

V

V

D

R

G

G

L

K

G

G

L

L

A

M

L

I

R

A

M

L

E

D

I

L

C

V

E

-

E

S

D

D

G

K

H

R

T

T

-

R

-

Q

-

P

-

L

-

D

P

P

N

A

K

A

A

G

L

Q

V

P

D

S

R

R

M

I

V

A

D

D

I

-

A

-

L

-

A

-

D

-

L

-

E

-

L

-

D

E

G

A

R

R

H

A

G

G

-

V

L

L

V

V

L

R

D

P

I

I

G

G

G

-

Y

-

K

-

N

N

V

K

V

I

T

I

L

L

A

S

P

P

S

P

L

L

L

T

I

L

T

T

K

R

S

D

E

E

I

A

D

G

Q

L

A

M

I

V

R

S

L

A

L

L

D

E

L

A

L

F

T

A

R

R

V

C

active site: Y148 (= Y161), D255 (= D275), K284 (= K304), T321 (= T336)
binding pyridoxal-5'-phosphate: G115 (= G133), S116 (= S134), Y148 (= Y161), H149 (= H162), G150 (= G163), E222 (= E242), D255 (= D275), V257 (≠ I277), K284 (= K304)

Q6LFH8 Ornithine aminotransferase; PfOAT; Ornithine--oxo-acid aminotransferase; EC 2.6.1.13 from Plasmodium falciparum (isolate 3D7) (see paper)
27% identity, 89% coverage: 45:465/475 of query aligns to 29:403/414 of Q6LFH8

query
sites
Q6LFH8

P

P

K

V

I

V

F

L

D

K

R

R

C

G

Q

K

G

G

S

V

Y

F

M

V

Y

Y

D

D

A

I

E

E

D

D

V

R

P

R

Y

Y

L

Y

D

D

L

F

Q

L

M

S

W

A

Y

Y

S

S

A

S

V

V

N

N

F

Q

G

G

Y

H

G

C

N

H

Q

P

R

D

L

I

N

L

K

N

A

A

L

M

T

I

R

N

Q

Q

L

A

D

K

R

K

L

L

P

-

Q

T

V

I

A

C

S

S

Q

R

Y

A

L

F

H

F

A

S

E

D

K

S

I

L

E

-

L

-

A

-

A

G

T

V

I

C

A

E

Q

R

H

Y

A

L

E

T

K

N

T

L

W

F

G

G

A

Y

G

D

G

K

R

V

V

L

H

M

F

M

N

N

V

T

G

G

G

-

S

A

Q

E

A

A

V

S

E

E

D

T

A

A

L

Y

K

K

L

L

-

C

-

R

-

K

-

W

-

G

-

Y

-

E

V

V

R

K

N

K

A

I

R

P

R

E

G

N

K

S

G

A

T

K

M

I

F

I

A

V

F

C

E

N

G

N

Y

F

H

S

G

G

R

R

T

T

L

L

G

G

A
x
C

S

V

A

S

I

A

T

S

S

T

S

D

Y

K

R

K

Y
x
C

R

K

R

N

Y

F

G

G

H

P

F

F

D

-

R

V

A

P

L

N

F

F

V

L

P

K

F

V

P

P

Y

Y

H

D

F

-

R

-

G

D

P

L

K

E

G

A

M

L

D

E

K

K

E

E

E

-

Y

-

G

-

L

-

H

-

C

-

V

-

K

-

Q

-

F

-

E

-

R

-

L

-

F

-

E

-

S

-

E

-

Y

-

N

-

G

-

V

L

W

Q

D

D

P

P

K

N

A

V

G

-

E

-

A

-

E

-

C

-

A

C

A

A

F

F

F

I

V

V

E

E

P

P

I

V

Q

Q

G

G

T

E

G

A

G

G

Y

V

V

I

I

V

P

P

P

S

H

D

N

S

F

Y

F

F

I

P

E

G

L

V

K

A

K

S

V

L

L

C

D

K

R

K

H

Y

G

N

I

V

L

L

L

F

V

V

V

A

D

D

E

E

I

V

Q

Q

M

T

G

G

V

L

Y

G

R

R

T

T

G

G

K

K

L

L

W

L

S

C

I

T

E

H

H

H

F

Y

G

G

V

V

T

K

P

P

D

D

V

V

L

I

V

L

F

L

G

G

K

K

A

A

I

L

T

S

N

G

G

G

L

H

N

Y

P

P

L

I

S

S

G

A

I

I

W

L

A

A

R

N

D

D

A

D

L

V

I

M

N

-

P

-

E

L

V

V

F

L

P

K

P

P

G

G

S

E

T

H

H

G

S

S

T

T

F

Y

A

G

S

G

N

N

P

P

L

L

G

A

T

A

A

A

V

I

A

C

L

V

E

E

T

A

L

L

R

K

M

V

V

L

E

I

E

N

E

E

Q

K

D

-

F

L

G
x
C

A

E

A

N

V

A

M

D

A

K

K

L

G

G

A

A

Y

P

F

F

L

L

E

Q

G

N

L

L

Q

K

Y

E

L

Q

Q

L

R

K

S

D

H

S

K

K

V

V

G

R

D

E

V

V

D

R

G

G

L

K

G

G

L

L

A

L

L
x
C

R

A

M

I

E

E

I

F

C

-

E

-

D

-

G

-

H

-

T

-

P

-

N

-

K

-

A

-

L

-

V

-

D

-

R

-

M

K

V

N

D

D

I

L

A

V

L

N

A

V

A

W

D

D

L

I

E

C

L

L

D

K

G

F

R

K

R

E

H

N

G

G

L

L

V

I

L

-

D

-

I

T

G

R

G

S

Y

V

Y

H

K

D

N

K

V

T

V

V

T

R

L

L

A

T

P

P

S

P

L

L

L
x
C

I

I

T

T

K

K

S

E

E

Q

I

L

D

D

Q

E

A

C

I

T

R

E

L

I

C154 (≠ A168) modified: Disulfide link with 163, Reversible; mutation to S: Severe reduction in catalytic activity. Does not affect TRX1-mediated activation. Severe reduction in catalytic activity and loss of TRX1-mediated activation; when associated with S-163.
C163 (≠ Y177) modified: Disulfide link with 154, Reversible; mutation to S: No effect on catalytic activity. Requires higher concentrations of TRX1 for activation. Severe reduction in catalytic activity and loss of TRX1-mediated activation; when associated with S-154.
C316 (≠ G361) mutation to S: About 70% reduction in catalytic activity. Does not affect TRX1-mediated activation.
C350 (≠ L395) mutation to S: About 70% reduction in catalytic activity. Does not affect TRX1-mediated activation.
C390 (≠ L452) mutation to S: About 70% reduction in catalytic activity. Does not affect TRX1-mediated activation.

Query Sequence

>WP_012172510.1 NCBI__GCF_000010525.1:WP_012172510.1
MNIPARHLAAGGTRPSAFDVSRETRLLALEQRYCSHGDTVHYSEPKIFDRCQGSYMYDAE
DVPYLDLQMWYSAVNFGYGNQRLNKALTRQLDRLPQVASQYLHAEKIELAATIAQHAEKT
WGAGGRVHFNVGGSQAVEDALKLVRNARRGKGTMFAFEGGYHGRTLGASAITSSYRYRRR
YGHFDRALFVPFPYHFRGPKGMDKEEYGLHCVKQFERLFESEYNGVWDPKAGEAECAAFF
VEPIQGTGGYVIPPHNFFIELKKVLDRHGILLVVDEIQMGVYRTGKLWSIEHFGVTPDVL
VFGKAITNGLNPLSGIWARDALINPEVFPPGSTHSTFASNPLGTAVALETLRMVEEEQDF
GAAVMAKGAYFLEGLQYLQRSHKVVGDVDGLGLALRMEICEEDGHTPNKALVDRMVDIAL
AADLELDGRRHGLVLDIGGYYKNVVTLAPSLLITKSEIDQAIRLLDLLLTRVTRG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory