SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012276264.1 NCBI__GCF_000019185.1:WP_012276264.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

P11551 L-fucose-proton symporter; 6-deoxy-L-galactose permease; L-fucose permease; L-fucose-H(+) symport protein from Escherichia coli (strain K12) (see 3 papers)
27% identity, 97% coverage: 3:428/437 of query aligns to 13:429/438 of P11551

query
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P11551

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W38 (≠ F28) mutation W->F,I,Y: Strong decrease in L-fucose transport.
D46 (≠ G36) Important for activity; mutation D->A,N: Loss of L-fucose transport.
E135 (≠ Q125) Important for activity; mutation E->A,D,Q: Loss of L-fucose transport.
W278 (vs. gap) mutation W->F,Y: Slight decrease in L-fucose transport.; mutation to I: 30% decrease in L-fucose transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Query Sequence

>WP_012276264.1 NCBI__GCF_000019185.1:WP_012276264.1
MTATTQAQASPKSSLLPMTIIGVLFFIFGFVTWLNGSLIPFLKIICELNEFQALFVTFAF
YIAYTVMALPMSSILKKTGYKNGMAIGLAIMVVGSLLFIPAAQSANFTLFLGALFVLGTG
LTILQTASNPYVVHIGPKESAAMRISIMGLINKGAGVIVPILFTALVLSGFENFTADHLA
ALSEADRLAQISELSSRLVMPYIYMAVALTFLIGLVKFSSLPELEFEAAEDHQEKGSITH
FPQVILGAVALFAYVGVEVIAGDTIGLYGESLGVHNFASLTSYTMVFMVFGYIIGVTCIP
KFISQEKALLGSAIAGILCIVGAALGSRESTLMADILWGWSGIPVIPNTVTFVALMGLAH
ALVWPSIWPLALEGLGKYTAQGSALLIMGISGGAILPLIFGKVAYFADNTQVAYWVGLPC
YLFILFYALKGHKMRSW

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory