SitesBLAST
Comparing WP_012282995.1 NCBI__GCF_000019165.1:WP_012282995.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 19 hits to proteins with known functional sites (download)
6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
49% identity, 99% coverage: 2:427/429 of query aligns to 2:429/431 of 6dzsA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), L12 (= L12), G13 (= G13), N14 (≠ T14), V15 (= V15), V45 (= V45), R46 (= R46), R47 (≠ N47), R52 (= R52), I63 (≠ P65), L78 (= L80), M79 (= M81), P84 (= P86), A102 (= A104), K104 (= K106), G306 (= G303), T310 (= T307)
Q5F8J4 NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
43% identity, 94% coverage: 23:427/429 of query aligns to 22:431/435 of Q5F8J4
- L45 (≠ R46) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
- LS 45:46 (≠ RN 46:47) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).
4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
50% identity, 74% coverage: 3:319/429 of query aligns to 3:307/402 of 4pg7A
Sites not aligning to the query:
6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
38% identity, 68% coverage: 5:296/429 of query aligns to 4:283/331 of 6a0sA
- active site: D191 (= D202), K195 (= K206)
- binding l-homoserine: K99 (= K106), N150 (= N160), G151 (= G161), T152 (= T162), Y178 (= Y189), E180 (= E191), D186 (= D197), K195 (= K206)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L12), G12 (= G13), T13 (= T14), V14 (= V15), L42 (= L44), V43 (= V45), R44 (= R46), D45 (≠ N47), K48 (= K50), R50 (= R52), A73 (≠ L80), M74 (= M81), A97 (= A104), K99 (= K106), G177 (= G188), E180 (= E191)
Sites not aligning to the query:
2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
38% identity, 68% coverage: 5:296/429 of query aligns to 4:283/331 of 2ejwA
6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
38% identity, 68% coverage: 5:296/429 of query aligns to 4:283/332 of 6a0tB
- active site: D191 (= D202), K195 (= K206)
- binding l-homoserine: N150 (= N160), G151 (= G161), T152 (= T162), Y178 (= Y189), E180 (= E191), D186 (= D197), K195 (= K206)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G11), G11 (≠ L12), G12 (= G13), T13 (= T14), V14 (= V15), L42 (= L44), V43 (= V45), R44 (= R46), D45 (≠ N47), K48 (= K50), R50 (= R52), A73 (≠ L80), M74 (= M81), G75 (= G82), A97 (= A104), N98 (= N105), G177 (= G188), E180 (= E191)
Sites not aligning to the query:
7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
37% identity, 56% coverage: 85:323/429 of query aligns to 81:300/300 of 7f4cA
Sites not aligning to the query:
- binding nadp nicotinamide-adenine-dinucleotide phosphate: 6, 7, 8, 9, 10, 11, 37, 38, 39, 72, 73, 74
5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
37% identity, 56% coverage: 85:323/429 of query aligns to 81:302/302 of 5x9dA
- active site: D196 (= D202), K200 (= K206)
- binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: P82 (= P86), T100 (≠ A104), N101 (= N105), K102 (= K106), G127 (≠ A131), S131 (≠ G135), N155 (= N160), G156 (= G161), T157 (= T162), Y183 (= Y189), A184 (= A190), E185 (= E191), D191 (= D197), D196 (= D202), K200 (= K206), A281 (= A302), G282 (= G303), A286 (≠ T307)
Sites not aligning to the query:
- binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: 6, 7, 8, 9, 10, 11, 37, 38, 72, 73, 74
4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
31% identity, 59% coverage: 64:316/429 of query aligns to 75:309/319 of 4xb1A
Sites not aligning to the query:
4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
31% identity, 59% coverage: 64:316/429 of query aligns to 75:309/319 of 4xb2A
- active site: D211 (= D202), K215 (= K206)
- binding l-homoserine: A171 (≠ G161), S172 (≠ T162), D206 (= D197), K215 (= K206)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V91 (≠ L80), S92 (≠ G82), S93 (≠ G83), S114 (≠ A104), N115 (= N105), K116 (= K106), S141 (≠ A131), G295 (≠ A302), T300 (= T307)
Sites not aligning to the query:
3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
29% identity, 53% coverage: 3:229/429 of query aligns to 1:236/319 of 3ingA
- active site: D209 (= D202), K213 (= K206)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G11), T10 (≠ L12), G11 (= G13), N12 (≠ T14), V13 (= V15), D38 (vs. gap), S39 (vs. gap), K57 (≠ D63), C85 (vs. gap), T86 (vs. gap), P87 (vs. gap), A112 (= A104), N113 (= N105), K114 (= K106), A139 (= A131), E198 (= E191), S199 (≠ A192)
Sites not aligning to the query:
3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
30% identity, 53% coverage: 3:229/429 of query aligns to 2:239/321 of 3jsaA
- active site: D212 (= D202), K216 (= K206)
- binding nicotinamide-adenine-dinucleotide: G10 (= G11), L11 (= L12), G12 (= G13), N13 (≠ T14), V14 (= V15), D42 (≠ R46), S43 (≠ N47), A90 (vs. gap), T91 (vs. gap), P92 (vs. gap), A117 (= A104), N118 (= N105), A144 (= A131)
Sites not aligning to the query:
P31116 Homoserine dehydrogenase; HDH; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
30% identity, 49% coverage: 5:214/429 of query aligns to 6:231/359 of P31116
- 11:18 (vs. 10:17, 50% identical) binding NADP(+)
- T93 (≠ M81) binding NADP(+)
- K117 (= K106) binding NADP(+)
- E208 (= E191) binding substrate; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
- D219 (= D202) mutation to L: Reduces kcat 150-fold.
- K223 (= K206) mutation to V: Loss of activity.
1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
30% identity, 49% coverage: 5:214/429 of query aligns to 5:230/358 of 1tveA
1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
30% identity, 49% coverage: 5:214/429 of query aligns to 5:230/358 of 1q7gA
- active site: D218 (= D202), K222 (= K206)
- binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: G13 (= G13), V14 (≠ T14), V15 (= V15), E39 (≠ Q39), N91 (≠ L80), T92 (≠ M81), S93 (≠ G82), I97 (≠ P86), P114 (≠ A104), K116 (= K106), A143 (= A131), S173 (≠ N160), K222 (= K206)
Sites not aligning to the query:
1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
30% identity, 49% coverage: 5:214/429 of query aligns to 5:230/358 of 1ebuD
- active site: D218 (= D202), K222 (= K206)
- binding 3-aminomethyl-pyridinium-adenine-dinucleotide: G11 (= G11), A12 (≠ L12), G13 (= G13), V14 (≠ T14), V15 (= V15), E39 (≠ Q39), A40 (≠ V40), N91 (≠ L80), S93 (≠ G82), K116 (= K106)
Sites not aligning to the query:
1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
30% identity, 49% coverage: 5:214/429 of query aligns to 5:230/358 of 1ebfA
- active site: D218 (= D202), K222 (= K206)
- binding nicotinamide-adenine-dinucleotide: I10 (≠ L10), A12 (≠ L12), G13 (= G13), V14 (≠ T14), V15 (= V15), E39 (≠ Q39), A40 (≠ V40), T92 (≠ M81), S93 (≠ G82), P114 (≠ A104)
O94671 Probable homoserine dehydrogenase; HDH; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 37% coverage: 58:214/429 of query aligns to 92:239/376 of O94671
- S201 (≠ D176) modified: Phosphoserine
O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 52% coverage: 95:319/429 of query aligns to 662:909/916 of O81852
Sites not aligning to the query:
- 441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
- 443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
- 522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
- 524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
Query Sequence
>WP_012282995.1 NCBI__GCF_000019165.1:WP_012282995.1
MKQPIMVGLLGLGTVGGGTLRILQNRVEDIAQRLGRPVQVKKVLVRNLHKPRPVVVDPAL
LTDNPADILEDPEISIVVELMGGIHPALDYIKSALKAGKNVVTANKDLMAVHGRELFDLA
EAKELDLEFEASVAGGIPIIRPLKVCLAANRIEELKGIINGTTNFILTKMTQEGSDFGEV
LKEAQALGYAEADPTADVEGLDAARKLAIVASIAFNSRITLNDVYVEGITKVTARDIQYA
RQMGYTIKLLGIAKEIDGKVEARVHPAMIPDCHPLAAVNDAFNAIFVKGDAVGETMFYGR
GAGDMPTGSAVVGDIIDVARNIIKGHTGRISCTCYHQKPVRPIGEIVCKYFLRLVVTEKP
GVLAAIAGVFGDQGVSIQTLIQQETIGDSAEIVLVTHPVREDNLQMALKILSDMPSVERV
GNVIRVERG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory