SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012283105.1 NCBI__GCF_000019165.1:WP_012283105.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
36% identity, 88% coverage: 3:301/339 of query aligns to 71:361/453 of Q9VLJ8

query
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Q9VLJ8

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Sites not aligning to the query:

62 modified: Phosphothreonine

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
39% identity, 72% coverage: 1:244/339 of query aligns to 10:248/270 of D4GSF3

query
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D4GSF3

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C188 (= C182) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
37% identity, 79% coverage: 3:269/339 of query aligns to 62:321/460 of O95396

query
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O95396

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158:238 (vs. 101:181, 37% identical) Interaction with NFS1
C239 (= C182) mutation to A: Impairs sulfurtransferase activity.
C316 (= C264) modified: Disulfide link with 324; mutation to A: Does not affect sulfurtransferase activity.

Sites not aligning to the query:

324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
38% identity, 78% coverage: 1:265/339 of query aligns to 8:269/271 of Q72J02

query
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Q72J02

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C192 (= C182) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.
C268 (= C264) mutation to S: Still able to form a thioester complex with TtuB.

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
38% identity, 73% coverage: 1:246/339 of query aligns to 9:249/249 of P12282

query
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P12282

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L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

I

T

L

L

Y

H

D

-

E

-

A

S

D

D

A

A

A

T

A

V

M

G

A

Q

P

P

K

K

A

V

V

E

A

S

A

A

K

R

Q

D

K

A

L

L

Q

T

A

R

I

I

N

N

S

P

E

H

I

I

Q

A

V

I

E

T

A

P

I

V

V

N

A

A

D

L

I

L

H

D

W

D

A

A

N

E

L

L

P

A

Q

A

L

L

L

I

D

A

G

E

V

H

D

D

L

L

V

V

L

L

D

D

G
x
C

S

T

D
|
D

N

N

F

V

D

A

L

V

R

R

Y

N

L

Q

L

L

N

N

D

A

A

G

C
|
C

V

F

Q

A

A

A

G

K

I

V

P

P

W

L

I

V

Y

S

G

G

G

A

V

A

T

I

G

R

A

M

H

E

G

G

M

Q

A

I

M

T

V

V

V

F

R

T

P

Y

G

Q

Q

D

G

G

-

E

P

P

C
|
C

L

Y

R

R

C
|
C

L

L

I

-

P

-

S

S

P

R

P

L

P

F

P

G

G

E

A

N

A

A

P

L

T

T

C
|
C

D

V

M

E

V

A

G

G

I

V

L

M

A

A

P

P

A

L

I

I

Q

G

M

V

I

I

T

G

A

S

F

L

Q

Q

A

A

V

M

E

E

A

A

M

I

K

K

L

M

L

L

A

A

G

G

R

Y

E

G

D

K

E

P

L

A

A

S

G

G

G

K

L

I

F

V

S

M

V

Y

D

D

L

A

W

M

N

T

S
x
C

R

Q

Y

F

D

R

L

E

I

M

D

K

L

L

T

M

A

-

R

R

R

N

D

P

D

G

C
|
C

P

E

A

V

C
|
C

S

G

R

Q

R14 (≠ K6) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ T36) mutation to A: No effect.
R73 (= R65) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ G122) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D124) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C136) mutation to A: No effect.
C172 (= C165) mutation to A: No zinc bound and no enzyme activity.
C175 (= C168) mutation to A: No zinc bound and no enzyme activity.
C187 (= C182) mutation to A: No effect.
C231 (≠ S226) mutation to A: No effect.
C244 (= C241) mutation to A: No zinc bound and almost no enzyme activity.
C247 (= C244) mutation to A: No zinc bound and almost no enzyme activity.

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
36% identity, 58% coverage: 14:209/339 of query aligns to 58:252/440 of P38820

query
sites
P38820

G

G

E

V

A

A

G

G

Q

Q

R

V

R

K

L

L

V

K

Q

N

S

T

T

K

V

V

L

L

V

V

A

V

G

G

V

A

G

G

A

G

L

L

G

G

T

C

H

P

L

A

A

L

N

P

T

Y

L

L

A

A

R

G

A

A

G

G

V

V

G

G

K

Q

L

I

V

G

L

I

V

V

D

D

R

N

D

D

F

V

V

V

E

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

L

L

Y

H

D

D

E

S

A

S

D

R

A

V

A

G

A

M

M

L

A

-

P

-

K

K

A

C

V

E

A

S

A

A

K

R

Q

Q

K

Y

L

I

Q

T

A

K

I

L

N

N

S

P

E

H

I

I

Q

N

V

V

E

V

A

T

I

Y

V

P

A

V

D

R

I

L

H

N

W

S

A

S

N

N

L

A

P

F

Q

D

L

I

L

F

D

K

G

G

V

Y

D

N

L

Y

V

I

L

L

D

D

G

C

S

T

D

D

N

S

F

P

D

L

L

T

R

R

Y

Y

L

L

L

V

N

S

D

D

A

V

C

A

V

V

Q

N

A

L

G

G

I

I

P

T

W

V

I

V

Y

S

G

A

G

S

V

G

T

L

G

G

A

T

H

E

G

G

M

Q

A

L

M

T

V

I

V

L

R

N

P

F

G

N

Q

N

-

I

G

G

P

P

C

C

L

Y

R

R

C

C

L

F

I

Y

P

P

S

T

P

P

G

N

A

A

A

V

P

T

T

S

C
|
C

D

Q

M

E

V

G

G

G

I

V

L

I

A

G

P

P

A

C

I

I

Q

G

M

L

I

V

T

G

A

T

F

M

Q

M

A

A

V

V

E

E

A

T

M

L

K

K

L

L

L

I

A

L

G

G

C225 (= C182) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
34% identity, 72% coverage: 3:245/339 of query aligns to 68:319/482 of O59954

query
sites
O59954

R

R

Y

Y

I

G

K

R

Q

Q

I

M

R

I

F

V

G

P

A

Q

F

F

G

G

E

L

A

Q

G
|
G

Q

Q

R

L

R

K

L

L

V

R

Q

D

S

A

T

K

V

V

L

L

V

I

A

V

G

G

V

A

G

G

A

G

L

L

G
|
G

T

C

H

P

L

A

A

A

N

L

T

Y

L

L

A

A

R

G

A

A

G

G

V

V

G

G

K

T

L

I

V

G

L

L

V

V

D

D

R

G

D

D

F

T

V

V

E

E

L

A

S

S

N

N

L

L

Q

H

R
|
R

Q

Q

I

V

L

L

Y

H

D

R

E

S

A

R

D

N

A

V

A

G

A

K

M

L

A

-

P

-

K

K

A

V

V

D

A

S

A

A

K

I

Q

E

K

Y

L

L

Q

R

A

E

I

L

N

N

S

P

E

H

I

P

Q

T

V

Y

E

I

A

A

I

H

V

Q

A

A

D

H

I

L

H

T

W

P

A

R

N

E

L

A

P

P

Q

D

L

I

L

F

D

K

G

D

V

Y

D

D

L

L

V

I

L

L

D

D

G
x
C

S

T

D

D

N

N

F

P

D

A

L

T

R

R

Y

Y

L

L

L

I

N

S

D

D

A

T

C

A

V

V

Q

L

A

L

G

G

I

K

P

P

W

L

I

V

Y

S

G

A

G

S

V

A

T

L

G

R

A

T

H
x
E

G

G

M

Q

A

L

M

M

V

V

V

L

R

N

-

N

-

P

-

Q

-

P

P

P

G

G

Q

D

-

K

-

T

-

G

G

G

P

P

C

C

L

Y

R

R

C

C

L

V

I

F

P

P

S

K

P

P

P

A

G

N

A

S

A

V

P

T

T

S

C

C

D

A

M

D

V

G

G

G

I

I

L

L

A

G

P

P

A

V

I

V

Q
x
G

M

T

I

M

T

G

A

V

F

L

Q

Q

A

A

V

S

E

E

A

A

M

I

K

K

L

V

L

L

A

T

G

S

R

A

E

G

D

E

E

S

L

V

A

E

G

A

G

T

L

P

F

P

S

S

V

L

D

L

L

I

W

F

N

S

S

A

-

Y

-

S

-

P

R

Q

Y

F

D

R

L

T

I

I

D

K

L

L

T

R

A

-

A

S

R

R

D

P

D

N

C

C

P

A

A

V

C

C

S

S

G82 (= G17) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G35) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R65) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ G122) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (≠ H152) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (≠ Q193) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
36% identity, 72% coverage: 1:244/339 of query aligns to 8:239/240 of 1jwbB

query
sites
1jwbB

M

M

E

L

R

R

Y

Y

I

N

K
x
R

Q

Q

I

I

R

I

F

L

G

R

A

G

F

F

G

D

E

F

A

D

G

G

Q

Q

R

E

R

A

L

L

V

K

Q

D

S

S

T

R

V

V

L

L

V

I

A

V

G
|
G

V

L

G
|
G

A
x
G

L

L

G

G

T

C

H

A

L

A

A

S

N

Q

T

Y

L

L

A

A

R

S

A

A

G

G

V

V

G

G

K

N

L

L

V

T

L

L

V

L

D
|
D

R
x
F

D

D

F

T

V

V

E

S

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

T

L

L

Y

H

D

-

E

-

A

S

D

D

A

A

A

T

A

V

M

G

A

Q

P

P

K
|
K

A

V

V

E

A

S

A

A

K

R

Q

D

K

A

L

L

Q

T

A

R

I

I

N

N

S

P

E

H

I

I

Q

A

V

I

E

T

A

P

I

V

V

N

A

A

D
x
L

I

L

H

D

W

D

A

A

N

E

L

L

P

A

Q

A

L

L

L

I

D

A

G

E

V

H

D

D

L

L

V

V

L

L

D

D

G
x
C

S
x
T

D
|
D

N
|
N

F

V

D

A

L
x
V

R

R

Y

N

L

Q

L

L

N

N

D

A

A

G

C

C

V

F

Q

A

A

A

G

K

I

V

P

P

W

L

I

V

Y

S

G

G

G

A

V

A

T

I

G

R

A

M

H

E

G

G

M

Q

A

I

M

T

V

V

V

F

R

T

P

Y

G

Q

Q

D

G

G

-

E

P

P

C
|
C

L

Y

R

R

C

C

L

L

I

-

P

-

S

-

P

-

G

-

A

-

A

S

P

R

T

L

C

F

D

G

M

E

V

A

G

G

I

V

L

M

A

A

P

P

A

L

I

I

Q

G

M

V

I

I

T

G

A

S

F

L

Q

Q

A

A

V

M

E

E

A

A

M

I

K

K

L

M

L

L

A

A

G

G

R

Y

E

G

D

K

E

P

L

A

A

S

G

G

G

K

L

I

F

V

S

M

V

Y

D

D

L

A

W

M

N

T

S

C

R

Q

Y

F

D

R

L

E

I

M

D

K

L

L

T

M

A

-

R

R

R

N

D

P

D

G

C
|
C

P

E

A

V

C
|
C

active site: R13 (≠ K6), D129 (= D124)
binding adenosine monophosphate: G37 (= G30), G39 (= G32), G40 (≠ A33), D61 (= D54), F62 (≠ R55), K85 (= K80), L108 (≠ D103), C127 (≠ G122), T128 (≠ S123), D129 (= D124), N130 (= N125), V133 (≠ L128)
binding zinc ion: C171 (= C165), C236 (= C241), C239 (= C244)

1jw9B Structure of the native moeb-moad protein complex (see paper)
36% identity, 72% coverage: 1:244/339 of query aligns to 8:239/240 of 1jw9B

query
sites
1jw9B

M

M

E

L

R

R

Y

Y

I

N

K
x
R

Q

Q

I

I

R

I

F

L

G

R

A

G

F

F

G

D

E

F

A

D

G

G

Q

Q

R

E

R

A

L

L

V

K

Q

D

S

S

T

R

V

V

L

L

V

I

A

V

G

G

V

L

G

G

A

G

L

L

G

G

T

C

H

A

L

A

A

S

N

Q

T

Y

L

L

A

A

R

S

A

A

G

G

V

V

G

G

K

N

L

L

V

T

L

L

V

L

D

D

R

F

D

D

F

T

V

V

E

S

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

T

L

L

Y

H

D

-

E

-

A

S

D

D

A

A

A

T

A

V

M

G

A

Q

P

P

K

K

A

V

V

E

A

S

A

A

K

R

Q

D

K

A

L

L

Q

T

A

R

I

I

N

N

S

P

E

H

I

I

Q

A

V

I

E

T

A

P

I

V

V

N

A

A

D

L

I

L

H

D

W

D

A

A

N

E

L

L

P

A

Q

A

L

L

L

I

D

A

G

E

V

H

D

D

L

L

V

V

L

L

D

D

G

C

S

T

D
|
D

N

N

F

V

D

A

L

V

R

R

Y

N

L

Q

L

L

N

N

D

A

A

G

C

C

V

F

Q

A

A

A

G

K

I

V

P

P

W

L

I

V

Y

S

G

G

G

A

V

A

T

I

G

R

A

M

H

E

G

G

M

Q

A

I

M

T

V

V

V

F

R

T

P

Y

G

Q

Q

D

G

G

-

E

P

P

C
|
C

L

Y

R

R

C

C

L

L

I

-

P

-

S

-

P

-

G

-

A

-

A

S

P

R

T

L

C

F

D

G

M

E

V

A

G

G

I

V

L

M

A

A

P

P

A

L

I

I

Q

G

M

V

I

I

T

G

A

S

F

L

Q

Q

A

A

V

M

E

E

A

A

M

I

K

K

L

M

L

L

A

A

G

G

R

Y

E

G

D

K

E

P

L

A

A

S

G

G

G

K

L

I

F

V

S

M

V

Y

D

D

L

A

W

M

N

T

S

C

R

Q

Y

F

D

R

L

E

I

M

D

K

L

L

T

M

A

-

R

R

R

N

D

P

D

G

C
|
C

P

E

A

V

C
|
C

active site: R13 (≠ K6), D129 (= D124)
binding zinc ion: C171 (= C165), C236 (= C241), C239 (= C244)

1zfnA Structural analysis of escherichia coli thif (see paper)
39% identity, 71% coverage: 3:244/339 of query aligns to 8:243/244 of 1zfnA

query
sites
1zfnA

R

R

Y

Y

I

S

K
x
R

Q

Q

I

I

R

L

F

L

G

D

A

D

F

I

G

A

E

L

A

D

G

G

Q

Q

R

Q

R

K

L

L

V

L

Q

D

S

S

T

Q

V

V

L

L

V

I

A
x
I

G
|
G

V

L

G
|
G

A
x
G

L

L

G

G

T

T

H

P

L

A

A

A

N

L

T

Y

L

L

A

A

R

G

A

A

G

G

V

V

G

G

K

T

L

L

V

V

L

L

V

A

D
|
D

R

D

D

D

F

D

V

V

E

H

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

I

I

L

L

Y

F

D

T

E

T

A

E

D

D

A

I

A

D

A

-

M

-

A

R

P

P

K
|
K

A

S

V

Q

A

V

A

S

K

Q

Q

Q

K

R

L

L

Q

T

A

Q

I

L

N

N

S

P

E

D

I

I

Q

Q

V

L

E

T

A

A

I

L

V

Q

A

Q

D

R

I

L

H

T

W

G

A

E

N

A

L

L

P

K

Q

D

L

A

L

V

D

A

G

R

V

A

D

D

L

V

V

V

L

L

D

D

G

C

S
x
T

D
|
D

N

N

F

M

D

A

L
x
T

R

R

Y

Q

L

E

L

I

N

N

D

A

A

A

C

C

V

V

Q

A

A

L

G

N

I

T

P

P

W

L

I

I

Y

T

G

A

G

S

V

A

T

V

G

G

A

F

H

G

G

G

M

Q

A

L

M

M

V

V

V

L

R

T

P

P

-

P

-

W

G

E

Q

Q

G

G

P

-

C
|
C

L

Y

R

R

C
|
C

L

L

I

W

P

P

S

D

P

N

P

Q

P

E

P

P

G

-

A

-

A

E

P

R

T

N

C

C

D

R

M

T

V

A

G

G

I

V

L

V

A

G

P

P

A

V

I

V

Q

G

M

V

I

M

T

G

A

T

F

L

Q

Q

A

A

V

L

E

E

A

A

M

I

K

K

L

L

A

S

G

G

R

I

E

E

D

T

E

P

L

-

A

A

G

G

G

E

L

L

F

R

S

L

V

F

D

D

L

G

W

K

N

S

S

S

R

Q

Y

W

D

R

L

-

I

-

D

S

L

L

T

A

A

L

A

R

R

R

R

A

D

S

D

G

C
|
C

P

P

A

V

C
|
C

active site: R11 (≠ K6), D127 (= D124)
binding adenosine-5'-triphosphate: I34 (≠ A29), G35 (= G30), G37 (= G32), G38 (≠ A33), D59 (= D54), R70 (= R65), Q71 (= Q66), K83 (= K80), T126 (≠ S123), D127 (= D124), T131 (≠ L128)
binding zinc ion: C169 (= C165), C172 (= C168), C240 (= C241), C243 (= C244)

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 71% coverage: 3:244/339 of query aligns to 8:243/251 of P30138

query
sites
P30138

R

R

Y

Y

I

S

K

R

Q

Q

I

I

R

L

F

L

G

D

A

D

F

I

G

A

E

L

A

D

G

G

Q

Q

R

Q

R

K

L

L

V

L

Q

D

S

S

T

Q

V

V

L

L

V

I

A

I

G

G

V

L

G

G

A

G

L

L

G

G

T

T

H

P

L

A

A

A

N

L

T

Y

L

L

A

A

R

G

A

A

G

G

V

V

G

G

K

T

L

L

V

V

L

L

V

A

D

D

R

D

D

D

F

D

V

V

E

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

I

L

L

Y

F

D

T

E

T

A

E

D

D

A

I

A

D

A

-

M

-

A

R

P

P

K

K

A

S

V

Q

A

V

A

S

K

Q

Q

Q

K

R

L

L

Q

T

A

Q

I

L

N

N

S

P

E

D

I

I

Q

Q

V

L

E

T

A

A

I

L

V

Q

A

Q

D

R

I

L

H

T

W

G

A

E

N

A

L

L

P

K

Q

D

L

A

L

V

D

A

G

R

V

A

D

D

L

V

V

V

L

L

D

D

G

C

S

T

D

D

N

N

F

M

D

A

L

T

R

R

Y

Q

L

E

L

I

N

N

D

A

A

A

C

C

V

V

Q

A

A

L

G

N

I

T

P

P

W

L

I

I

Y

T

G

A

G

S

V

A

T

V

G

G

A

F

H

G

G

G

M

Q

A

L

M

M

V

V

V

L

R

T

P

P

-

P

-

W

G

E

Q

Q

G

G

P

-

C
|
C

L

Y

R

R

C
|
C

L

L

I
x
W

P

P

S

D

P

N

P

Q

P

E

P

P

G

-

A

-

A

E

P

R

T

N

C
|
C

D

R

M

T

V

A

G

G

I

V

L

V

A

G

P

P

A

V

I

V

Q

G

M

V

I

M

T

G

A

T

F

L

Q

Q

A

A

V

L

E

E

A

A

M

I

K

K

L

L

A

S

G

G

R

I

E

E

D

T

E

P

L

-

A

A

G

G

G

E

L

L

F

R

S

L

V

F

D

D

L

G

W

K

N

S

S

S

R

Q

Y

W

D

R

L

-

I

-

D

S

L

L

T

A

A

L

A

R

R

R

R

A

D

S

D

G

C
|
C

P

P

A

V

C
|
C

C169 (= C165) binding Zn(2+)
C172 (= C168) binding Zn(2+)
W174 (≠ I170) mutation to A: No adenylation of ThiS.
C184 (= C182) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
C240 (= C241) binding Zn(2+)
C243 (= C244) binding Zn(2+)

1zud3 Structure of this-thif protein complex (see paper)
39% identity, 71% coverage: 3:244/339 of query aligns to 8:238/240 of 1zud3

query
sites
1zud3

R

R

Y

Y

I

S

K
x
R

Q

Q

I

I

R
x
L

F

L

G
x
D

A

D

F

I

G

A

E

L

A

D

G

G

Q

Q

R

Q

R

K

L

L

V

L

Q

D

S

S

T

Q

V

V

L

L

V

I

A

I

G

G

V

L

G

G

A

G

L

L

G

G

T

T

H

P

L

A

A

A

N

L

T

Y

L

L

A

A

R

G

A

A

G

G

V

V

G

G

K

T

L

L

V

V

L

L

V

A

D

D

R

D

D

D

F

D

V

V

E

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

I

L

L

Y

F

D

T

E

T

A

E

D

D

A

I

A

D

A

-

M

-

A

R

P

P

K

K

A

S

V

Q

A

V

A

S

K

Q

Q

Q

K

R

L

L

Q

T

A

Q

I

L

N

N

S

P

E

D

I

I

Q

Q

V

L

E

T

A

A

I

L

V

Q

A

Q

D

R

I

L

H

T

W

G

A

E

N

A

L

L

P

K

Q

D

L

A

L

V

D

A

G

R

V

A

D

D

L

V

V

V

L

L

D

D

G

C

S

T

D
|
D

N

N

F

M

D

A

L

T

R

R

Y

Q

L

E

L

I

N

N

D

A

A

A

C

C

V

V

Q

A

A

L

G

N

I

T

P

P

W

L

I

I

Y

T

G

A

G

S

V

A

T

V

G

G

A

F

H

G

G

G

M

Q

A

L

M

M

V

V

V

L

R

T

P

P

-

P

-

W

G

E

Q

Q

G

G

P

-

C
|
C

L

Y

R

R

C

C

L

L

I

W

P

P

S

D

P

N

P

Q

P

E

P

P

G

T

A

A

A

G

P

V

T

V

C

-

D

-

M

-

V

-

G

-

I

-

L

-

A

G

P

P

A

V

I

V

Q

G

M

V

I

M

T

G

A

T

F

L

Q

Q

A

A

V

L

E

E

A

A

M

I

K

K

L

L

A

S

G

G

R

I

E

E

D

T

E

P

L

-

A

A

G

G

G

E

L

L

F

R

S

L

V

F

D

D

L

G

W

K

N

S

S

S

R

Q

Y

W

D

R

L

-

I

-

D

S

L

L

T

A

A

L

A

R

R

R

R

A

D

S

D

G

C
|
C

P

P

A

V

C
|
C

active site: R11 (≠ K6), D127 (= D124)
binding calcium ion: L14 (≠ R9), D16 (≠ G11)
binding zinc ion: C169 (= C165), C235 (= C241), C238 (= C244)

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
35% identity, 67% coverage: 1:228/339 of query aligns to 8:210/217 of 1jwaB

query
sites
1jwaB

M

M

E

L

R

R

Y

Y

I

N

K
x
R

Q

Q

I

I

R

I

F

L

G

R

A

G

F

F

G

D

E

F

A

D

G

G

Q

Q

R

E

R

A

L

L

V

K

Q

D

S

S

T

R

V

V

L

L

V

I

A

V

G

G

V

L

G
|
G

A
x
G

L

L

G

G

T

C

H

A

L

A

A

S

N

Q

T

Y

L

L

A

A

R

S

A

A

G

G

V

V

G

G

K

N

L

L

V

T

L

L

V

L

D
|
D

R
x
F

D

D

F

T

V

V

E

S

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

I

T

L

L

Y

H

D

-

E

-

A

S

D

D

A

A

A

T

A

V

M

G

A

Q

P

P

K
|
K

A

V

V

E

A

S

A

A

K

R

Q

D

K

A

L

L

Q

T

A

R

I

I

N

N

S

P

E

H

I

I

Q

A

V

I

E

T

A

P

I

V

V

N

A

A

D
x
L

I

L

H

D

W

D

A

A

N

E

L

L

P

A

Q

A

L

L

L

I

D

A

G

E

V

H

D

D

L

L

V

V

L

L

D

D

G

C

S

T

D
|
D

N
|
N

F

V

D

A

L
x
V

R

R

Y

N

L

Q

L

L

N

N

D

A

A

G

C

C

V

F

Q

A

A

A

G

K

I

V

P

P

W

L

I

V

Y

S

G

G

G

A

V

A

T

I

G

R

A

M

H

E

G

G

M

Q

A

I

M

T

V

V

V

F

R

T

P

Y

G

E

Q

-

G

-

P

-

C

-

L

-

R

-

C

-

L

-

I

-

P

-

S

-

P

-

G

-

A

-

P

-

T

-

C

-

D

-

M

-

V

A

G

G

I

V

L

M

A

A

P

P

A

L

I

I

Q

G

M

V

I

I

T

G

A

S

F

L

Q

Q

A

A

V

M

E

E

A

A

M

I

K

K

L

M

L

L

A

A

G

G

R

Y

E

G

D

K

E

P

L

A

A

S

G

G

G

K

L

I

F

V

S

M

V

Y

D

D

L

A

W

M

N

T

S

C

R

Q

Y

F

active site: R13 (≠ K6), D129 (= D124)
binding adenosine-5'-triphosphate: G39 (= G32), G40 (≠ A33), D61 (= D54), F62 (≠ R55), R72 (= R65), K85 (= K80), L108 (≠ D103), D129 (= D124), N130 (= N125), V133 (≠ L128)

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
32% identity, 75% coverage: 1:254/339 of query aligns to 12:278/289 of 6yubB

query
sites
6yubB

M

I

E

E

R

R

Y

Y

I

S

K

R

Q

Q

I

M

R

I

F

V

G

P

A

G

F

M

G

G

E

K

A

E

G

G

Q

Q

R

L

R

R

L

L

V

M

Q

N

S

A

T

K

V

V

L

L

V

I

A

I

G

G

V

A

G

G

A

G

L

L

G

G

T

C

H

P

L

A

A

A

N

Q

T

Y

L

L

A

A

R

G

A

A

G

G

V

V

G

G

K

T

L

I

V

G

L

I

V

V

D

D

R

G

D

D

F

S

V

V

E

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

L

-

Y

-

D

-

E

-

A

-

D

-

A

-

A

A

M

H

A

A

P

T

K

K

A

R

V

V

A

G

A

M

K

L

Q

K

K

V

L

D

Q

S

A

L

I

I

N

T

S

H

E

L

I

I

Q

E

V

I

E

N

A

P

I

L

V

P

A

V

-

Y

-

V

-

P

-

Y

-

R

-

F

D

D

I

L

H

T

W

P

A

Q

N

N

L

A

P

A

Q

Q

L

I

D

K

G

P

V

W

D

D

L

V

V

I

L

L

D

D

G

C

S

T

D

D

N

N

F

P

D

A

L

T

R

R

Y

Y

L

L

L

I

N

S

D

D

A

V

C

C

V

V

Q

L

A

L

G

G

I

K

P

P

W

L

I

V

Y

-

G

-

V

-

T

-

G

-

A

-

H

-

G

S

M

A

A

A

M

S

V

V

V

Q

R

K

P

S

G

G

Q

Q

G

L

P

I

C

V

L

L

R

N

C

C

L

-

I

-

P

-

S

-

P

P

P

T

P

P

-

Q

-

G

-

V

-

N

-

K

G

K

A

A

P

P

T
x
C

-

Y

-

R

C
|
C

D

C

M

F

V

K

G

G

I

I

L

M

A

G

P

P

A

V

I

V

Q

G

M

M

I

M

T

G

A

V

F

A

Q

Q

A

A

V

G

E

E

A

A

M

I

K

K

L

I

L

L

A

V

-

S

-

Q

-

L

-

H

-

M

-

P

-

K

-

E

G

G

R

E

E

E

D

V

E

S

L

P

A

E

G

K

G

N

L

L

F

V

S

Q

V

P

D

T

L

L

W

L

N

I

S

Y

R

T

Y

Y

D

D

L

L

I

N

D

S

L

A

T

I

-

G

-

P

-

Y

-

S

-

F

-

R

-

A

-

L

-

K

-

M

A

G

R

R

R

K

D

K

D

D

C
|
C

P

F

A

A

C

C

S

G

R

E

R

N

D

S

F

T

P

L

F

T

L

L

A

D

G

G

binding zinc ion: C184 (≠ T181), C187 (= C182), C265 (= C241)

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
30% identity, 75% coverage: 1:254/339 of query aligns to 11:279/423 of 6yubA

query
sites
6yubA

M

I

E

E

R

R

Y

Y

I

S

K

R

Q

Q

I

M

R

I

F

V

G

P

A

G

F

M

G

G

E

K

A

E

G

G

Q

Q

R

L

R

R

L

L

V

M

Q

N

S

A

T

K

V

V

L

L

V

I

A

I

G

G

V

A

G

G

A

G

L

L

G

G

T

C

H

P

L

A

A

A

N

Q

T

Y

L

L

A

A

R

G

A

A

G

G

V

V

G

G

K

T

L

I

V

G

L

I

V

V

D

D

R

G

D

D

F

S

V

V

E

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

L

-

Y

-

D

-

E

-

A

-

D

-

A

-

A

A

M

H

A

A

P

T

K

K

A

R

V

V

A

G

A

M

K

L

Q

K

K

V

L

D

Q

S

A

L

I

I

N

T

S

H

E

L

I

I

Q

E

V

I

E

N

A

P

I

L

V

P

A

V

-

Y

-

V

-

P

-

Y

-

R

-

F

D

D

I

L

H

T

W

P

A

Q

N

N

L

A

P

A

Q

Q

L

I

D

K

G

P

V

W

D

D

L

V

V

I

L

L

D

D

G

C

S

T

D

D

N

N

F

P

D

A

L

T

R

R

Y

Y

L

L

L

I

N

S

D

D

A

V

C

C

V

V

Q

L

A

L

G

G

I

K

P

P

W

L

I

V

Y

S

G

A

V

S

T

V

G

Q

A

K

H

S

G

G

M

Q

A

L

M

I

-

V

-

L

-

N

-

C

-

P

-

T

-

P

-

Q

-

G

V

V

R

N

P

K

G

K

Q

A

G

A

P

P

C
|
C

L

Y

R

R

C
|
C

L

C

I

F

P

K

S

K

P

P

P

-

G

-

A

-

P

-

T

-

C

-

D

-

M

-

V

-

G

G

I

I

L

M

A

G

P

P

A

V

I

V

Q

G

M

M

I

M

T

G

A

V

F

A

Q

Q

A

A

V

G

E

E

A

A

M

I

K

K

L

I

L

L

A

V

-

S

-

Q

-

L

-

H

-

M

-

P

-

K

-

E

G

G

R

E

E

E

D

V

E

S

L

P

A

E

G

K

G

N

L

L

F

V

S

Q

V

P

D

T

L

L

W

L

N

I

S

Y

R

T

Y

Y

D

D

L

L

I

N

D

S

L

A

T

I

-

G

-

P

-

Y

-

S

-

F

-

R

-

A

-

L

-

K

-

M

A

G

R

R

R

K

D

K

D

D

C
|
C

P

F

A

A

C
|
C

S

G

R

E

R

N

D

S

F

T

P

L

F

T

L

L

A

D

G

G

binding zinc ion: C183 (= C165), C186 (= C168), C266 (= C241), C269 (= C244)

O42939 Ubiquitin-activating enzyme E1-like; Pmt3-activating enzyme subunit 2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
32% identity, 48% coverage: 19:182/339 of query aligns to 21:181/628 of O42939

query
sites
O42939

R

R

R

N

L

F

V

K

Q

S

S

A

T

K

V

V

L

L

V

L

A

V

G

G

V

A

G

G

A

G

L

I

G

G

T

C

H

E

L

L

A

L

N

K

T

N

L

L

A

L

R

M

A

S

G

G

V

V

G

K

K

E

L

V

V

H

L

I

V

I

D

D

R

L

D

D

F

T

V

I

E

D

L

L

S

S

N

N

L

L

Q

N

R

R

Q

Q

I

F

L

L

Y

F

D

R

E

K

A

K

D

H

A

V

A

K

A

Q

M

-

A

-

P

P

K

K

A

A

V

I

A

V

A

A

K

A

Q

K

K

T

L

A

Q

S

A

S

I

F

N

N

S

P

E

N

I

V

Q

K

V

L

E

E

A

A

I

Y

V

H

A

A

D

N

I

I

H

K

W

E

A

D

N

R

L

F

P

N

-

V

Q

A

L

W

L

F

D

R

G

Q

V

F

D

D

L

L

V

V

L

F

D

N

G

A

S

L

D

D

N

N

F

L

D

D

L

A

R

R

Y

R

L

H

L

V

N

N

D

K

A

Q

C

C

V

L

Q

L

A

A

G

S

I

V

P

P

W

L

I

I

Y

E

G

S

G

G

V

T

T

T

G

G

A

F

H

L

G

G

M

Q

A

V

M

Q

V

V

V

I

R

I

P

H

G

G

Q

K

G

T

P

E

C

C

L

Y

R

D

C

C

L

-

I

-

P

-

S

N

P

P

P

K

-

E

P

P

G

K

A

T

A

Y

P

P

T

V

C

C

Sites not aligning to the query:

559 modified: Phosphoserine
563 modified: Phosphoserine

3h9jA Crystal structure of e. Coli mccb + ampcpp + semet mcca (see paper)
25% identity, 69% coverage: 10:244/339 of query aligns to 102:337/339 of 3h9jA

query
sites
3h9jA

F

Y

G

G

A

A

F

N

G

P

E

V

A

L

G

V

Q

Q

R

D

R

K

L

L

V

K

Q

N

S

A

T

K

V

V

L

V

V

I

A

L

G

G

V

C

G
|
G

A
x
G

L

I

G

G

T

N

H

H

L

V

A

S

N

V

T

I

L

L

A

A

R

T

A

S

G

G

V

I

G

G

K

E

L

I

V

I

L

L

V

I

D
|
D

R

N

D
|
D

F

Q

V

I

E

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

I

V

L

L

Y

F

D

S

E

E

A

D

D

D

A

V

A

G

A

K

M

N

A
x
K

P

T

K

E

A

V

V

I

A

-

K

K

Q

R

K

E

L

L

Q

L

A

K

I

R

N

N

S

S

E

E

I

I

Q

S

V

V

E

S

A

E

I

I

V

A

A

L

D
x
N

I
|
I

H

N

-

D

W

Y

A

T

N

D

L

L

P

H

Q

K

L

V

L

P

D

E

G

A

V

D

D

I

L

W

V

V

L

V

D

S

G
x
A

S
x
D

D
x
H

N

P

F

F

D

N

L

L

R

I

Y

N

L

W

L

V

N

N

D

K

A

Y

C

C

V

V

Q

R

A

A

G

N

I

Q

P

P

W

Y

I

I

Y

N

G

A

G

G

-

Y

V

V

T

N

G

D

A

I

H

A

G

V

M

F

A

G

M

P

V

L

V

Y

R

V

P

P

G

G

Q

K

G

T

P

G

C
|
C

L

Y

R

E

C

C

-

Q

-

K

-

E

-

N

-

I

-

D

-

H

-

K

-

I

-

K

L

L

I

I

P

N

S

S

P

R

P

F

P

K

P

P

G

A

A

T

A

-

P

-

T

-

C

-

D

-

M

-

V

-

G

-

I

-

L

F

A

A

P

P

A

V

I

N

Q

N

M

V

I

A

T

A

A

A

F

L

Q

C

A

A

V

A

E

D

A

V

M

I

K

K

L

F

L

I

A

G

G

K

R

Y

E

S

D

E

E

P

L

L

A

S

G

L

G

N

L

K

F

-

S
x
R

V

I

D
x
G

L

I

W

W

N

S

S

D

R

E

Y

I

D

K

L

I

I

H

D

S

L

Q

T

N

A

M

A

G

R

R

R

S

D

P

D

V

C
|
C

P

S

A

V

C
|
C

active site: R157 (= R65)
binding diphosphomethylphosphonic acid adenosyl ester: G124 (= G32), G125 (≠ A33), D146 (= D54), D148 (= D56), R157 (= R65), K170 (≠ A78), N193 (≠ D103), I194 (= I104), A213 (≠ G122), D214 (≠ S123), H215 (≠ D124)
binding zinc ion: C257 (= C165), C334 (= C241), C337 (= C244)
binding : R313 (≠ S220), G315 (≠ D222)

3h5nA Crystal structure of e. Coli mccb + atp (see paper)
25% identity, 69% coverage: 10:244/339 of query aligns to 102:337/338 of 3h5nA

query
sites
3h5nA

F

Y

G

G

A

A

F

N

G

P

E

V

A

L

G

V

Q

Q

R

D

R

K

L

L

V

K

Q

N

S

A

T

K

V

V

L

V

V

I

A

L

G

G

V

C

G
|
G

A

G

L

I

G

G

T

N

H

H

L

V

A

S

N

V

T

I

L

L

A

A

R

T

A

S

G

G

V

I

G

G

K

E

L

I

V

I

L

L

V

I

D
|
D

R

N

D

D

F

Q

V

I

E

E

L

N

S

T

N
|
N

L

L

Q

T

R
|
R

Q
|
Q

I

V

L

L

Y

F

D

S

E

E

A

D

D

D

A

V

A

G

A

K

M

N

A
x
K

P

T

K

E

A

V

V

I

A

-

K

K

Q

R

K

E

L

L

Q

L

A

K

I

R

N

N

S

S

E

E

I

I

Q

S

V

V

E

S

A

E

I

I

V

A

A

L

D
x
N

I
|
I

H

N

-

D

W

Y

A

T

N

D

L

L

P

H

Q

K

L

V

L

P

D

E

G

A

V

D

D

I

L

W

V

V

L

V

D

S

G
x
A

S
x
D

D
x
H

N

P

F

F

D

N

L

L

R

I

Y

N

L

W

L

V

N

N

D

K

A

Y

C

C

V

V

Q

R

A

A

G

N

I

Q

P

P

W

Y

I

I

Y

N

G

A

G

G

-

Y

V

V

T

N

G

D

A

I

H

A

G

V

M

F

A

G

M

P

V

L

V

Y

R

V

P

P

G

G

Q

K

G

T

P

G

C
|
C

L

Y

R

E

C
|
C

-

Q

-

K

-

E

-

N

-

I

-

D

-

H

-

K

-

I

-

K

L

L

I

I

P

N

S

S

P

R

P

F

P

K

P

P

G

A

A

T

A

-

P

-

T

-

C

-

D

-

M

-

V

-

G

-

I

-

L

F

A

A

P

P

A

V

I

N

Q

N

M

V

I

A

T

A

A

A

F

L

Q

C

A

A

V

A

E

D

A

V

M

I

K

K

L

F

L

I

A

G

G

K

R

Y

E

S

D

E

E

P

L

L

A

S

G

L

G

N

L

K

F

-

S

R

V

I

D

G

L

I

W

W

N

S

S

D

R

E

Y

I

D

K

L

I

I

H

D

S

L

Q

T

N

A

M

A

G

R

R

R

S

D

P

D

V

C
|
C

P

S

A

V

C
|
C

active site: R157 (= R65)
binding adenosine-5'-triphosphate: G124 (= G32), D146 (= D54), N154 (= N62), R157 (= R65), Q158 (= Q66), K170 (≠ A78), N193 (≠ D103), I194 (= I104), A213 (≠ G122), D214 (≠ S123), H215 (≠ D124)
binding zinc ion: C257 (= C165), C260 (= C168), C334 (= C241), C337 (= C244)

3h5rA Crystal structure of e. Coli mccb + succinimide (see paper)
25% identity, 69% coverage: 10:244/339 of query aligns to 102:338/340 of 3h5rA

query
sites
3h5rA

F

Y

G

G

A

A

F

N

G

P

E

V

A

L

G

V

Q

Q

R

D

R

K

L

L

V

K

Q

N

S

A

T

K

V

V

L

V

V

I

A

L

G

G

V

C

G

G

A

G

L
x
I

G

G

T

N

H

H

L

V

A

S

N

V

T

I

L

L

A

A

R

T

A

S

G

G

V

I

G

G

K

E

L

I

V

I

L

L

V

I

D

D

R

N

D

D

F

Q

V

I

E

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

I

V

L

L

Y

F

D

S

E

E

A

D

D

D

A

V

A

G

A

K

M

N

A

K

P

T

K

E

A

V

V

I

A

-

K

K

Q

R

K

E

L

L

Q

L

A

K

I

R

N

N

S

S

E

E

I

I

Q

S

V

V

E

S

A

E

I

I

V

A

A

L

D

N

I

I

H

N

-

D

W

Y

A

T

N

D

L

L

P

H

Q

K

L

V

L

P

D

E

G

A

V

D

D

I

L

W

V

V

L

V

D
x
S

G
x
A

S

D

D

H

N

P

F

F

D

N

L

L

R

I

Y

N

L

W

L

V

N

N

D

K

A

Y

C

C

V

V

Q

R

A

A

G

N

I

Q

P

P

W

Y

I

I

Y

N

G
x
A

G
|
G

-
x
Y

V

V

T

N

G

D

A

I

H

A

G
x
V

M

F

A

G

M

P

V

L

V

Y

R

V

P

P

G

G

Q

K

G

T

P

G

C
|
C

L

Y

R

E

C
|
C

-

Q

-

K

-

V

-

K

-

E

-

N

-

I

-

D

-

H

-

K

-

I

-

K

L

L

I

I

P

N

S

S

P

R

P

F

P

K

P
|
P

G

A

A

T

A

-

P

-

T

-

C

-

D

-

M

-

V

-

G

-

I

-

L

F

A

A

P

P

A

V

I

N

Q

N

M

V

I

A

T

A

A

A

F

L

Q

C

A

A

V

A

E

D

A

V

M

I

K

K

L

F

L

I

A

G

G

K

R

Y

E

S

D

E

E

P

L

L

A

S

G

L

G

N

L

K

F

-

S
x
R

V

I

D
x
G

L

I

W

W

N

S

S

D

R

E

Y

I

D

K

L

I

I

H

D

S

L

Q

T

N

A

M

A

G

R

R

R

S

D

P

D

V

C
|
C

P

S

A

V

C
|
C

active site: R157 (= R65)
binding zinc ion: C257 (= C165), C260 (= C168), C335 (= C241), C338 (= C244)
binding : I126 (≠ L34), S212 (≠ D121), A213 (≠ G122), A237 (≠ G146), G238 (= G147), Y239 (vs. gap), V245 (≠ G153), P280 (= P176), R314 (≠ S220), G316 (≠ D222)

Sites not aligning to the query:

binding : 10, 14, 26

3h5aC Crystal structure of e. Coli mccb (see paper)
30% identity, 47% coverage: 10:168/339 of query aligns to 102:260/358 of 3h5aC

query
sites
3h5aC

F

Y

G

G

A

A

F

N

G

P

E

V

A

L

G

V

Q

Q

R

D

R

K

L

L

V

K

Q

N

S

A

T

K

V

V

L

V

V

I

A

L

G

G

V

C

G

G

A

G

L

I

G

G

T

N

H

H

L

V

A

S

N

V

T

I

L

L

A

A

R

T

A

S

G

G

V

I

G

G

K

E

L

I

V

I

L

L

V

I

D

D

R

N

D

D

F

Q

V

I

E

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

I

V

L

L

Y

F

D

S

E

E

A

D

D

D

A

V

A

G

A

K

M

N

A

K

P

T

K

E

A

V

V

I

A

-

K

K

Q

R

K

E

L

L

Q

L

A

K

I

R

N

N

S

S

E

E

I

I

Q

S

V

V

E

S

A

E

I

I

V

A

A

L

D

N

I

I

H

N

-

D

W

Y

A

T

N

D

L

L

P

H

Q

K

L

V

L

P

D

E

G

A

V

D

D

I

L

W

V

V

L

V

D

S

G

A

S

D

D

H

N

P

F

F

D

N

L

L

R

I

Y

N

L

W

L

V

N

N

D

K

A

Y

C

C

V

V

Q

R

A

A

G

N

I

Q

P

P

W

Y

I

I

Y

N

G

A

G

G

-

Y

V

V

T

N

G

D

A

I

H

A

G

V

M

F

A

G

M

P

V

L

V

Y

R

V

P

P

G

G

Q

K

G

T

P

G

C
|
C

L

Y

R

E

C
|
C

active site: R157 (= R65)
binding zinc ion: C257 (= C165), C260 (= C168)

Sites not aligning to the query:

binding zinc ion: 343

Query Sequence

>WP_012283105.1 NCBI__GCF_000019165.1:WP_012283105.1
MERYIKQIRFGAFGEAGQRRLVQSTVLVAGVGALGTHLANTLARAGVGKLVLVDRDFVEL
SNLQRQILYDEADAAAMAPKAVAAKQKLQAINSEIQVEAIVADIHWANLPQLLDGVDLVL
DGSDNFDLRYLLNDACVQAGIPWIYGGVTGAHGMAMVVRPGQGPCLRCLIPSPPPPGAAP
TCDMVGILAPAIQMITAFQAVEAMKLLAGREDELAGGLFSVDLWNSRYDLIDLTAARRDD
CPACSRRDFPFLAGREEMQATPLCGSNAVQITPARPAELDLEAMSERLSGAGKVEVTPYL
LRFASAEGEMVLFRDGRAMIRGTQDPVKAKAVYMRFIGA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory