SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012283382.1 NCBI__GCF_000019165.1:WP_012283382.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
67% identity, 100% coverage: 3:442/442 of query aligns to 3:443/443 of 4lnkA

query
sites
4lnkA

Y

Y

T

T

K

R

E

E

D

D

V

I

L

E

R

K

M

L

A

V

R

K

E

E

F

E

D

N

V

V

K

K

F

Y

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

L

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

I

P

P

V

V

D

S

Q

Q

L

L

Q

G

K
|
K

A

A

L

L

D

D

G

N

E

K

M

V

M
|
M

F

F

D
|
D

G

G

S

S

I

I

D

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

Y

P

P

D

D

P

L

N

N

T

T

F

F

V

V

I

I

F

F

P

P

W

W

R

T

P

A

Q

E

D

K

G

G

A

K

V

V

A

A

R

R

L

F

I

I

C

C

D

D

V

I

Y

Y

N

N

P

P

D

D

G

G

T

T

P

P

F

F

A

E

G

G

D

D

P

P

R

R

N

N

V

N

L

L

K

K

S

R

V

I

L

L

A

K

E

E

A

M

A

E

E

D

M

L

G

G

Y

F

T

S

-

D

M

F

N

N

V

L

G

G

P

P

E
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E

L

P

E
|
E

F

F

L

L

F

F

L

K

T

L

D

D

A

E

E

K

G

G

K

E

P

P

T

T

T

L

I

E

T

L

H

N

D

D

K

K

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

T

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D

L

L

G

G

E

E

N

N

A

C

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R

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D

M

I

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L

L

T

E

L

L

E

E

Q

E

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M

G

G

F

F

E

E

I

I

E

E

A

A

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S

H

H

E
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E

V
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V

A

A

A

P

G

G

Q

Q

H

H

E
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E

I

I

D

D

F
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F

K

K

Y
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Y

S

A

D

G

A

A

L

V

D

R

V

S

A

C

D

D

K

D

I

I

V

Q

T

T

F

F

K

K

F

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G

G

I

V

N
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N

G
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G

S

S

G
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G

M

M

H
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H

T

C

N
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N

M

L

S
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S

L

L

F

F

K

K

D

N

G

G

K

V

N

N

A

A

F

F

Y

F

D

D

P

E

T

N

T

A

P

D

D

L

Q

Q

L

L

S

S

E

E

T

T

A

A

K

K

Y

H

Y

F

I

I

G

A

G

G

L

I

L

V

K

K

N

H

V

A

R

T

S

S

F

F

A

T

A

A

I

V

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

Y

Y

E
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E

A

A

P

P

C

C

Y

Y

V

V

A

A

W

W

S

S

C

A

R

Q

N

N

R
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R

S

S

P

P

L

L

I

I

R

R

I

I

P

P

A

A

K
x
S

R

R

G

G

A

I

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S

T

T

R
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R

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V

E
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E

L

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R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

V

V

C

L

L

L

K

A

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

N

K

K

I

L

M

E

P

A

P

P

A

A

P

P

V

I

D

D

E

R

N

N

I

I

Y

Y

H

V

M

M

D

S

A

K

A

E

R

E

R

R

A

M

E

E

I

N

G

G

I

I

V

V

S

D

L

L

P

P

A

A

N

T

L

L

A

A

E

E

A

A

I

L

D

E

E

E

L

F

E

K

K

S

S

N

E

E

V

V

I

M

K

V

E

K

A

A

L

L

G

G

P

E

H

H

V

L

S

F

E

E

R

H

Y

F

I

I

E

E

A

A

K

K

R

E

E

I

E

E

W

W

D

D

S

M

F

F

R

R

I

T

T

Q

V

V

H

H

P

P

W

W

E

E

V

R

E

E

E

Q

Y

Y

L

M

T

S

K

Q

Y

Y

active site: D52 (= D52), E131 (= E130), E133 (= E132), E188 (= E187), E195 (= E194), H244 (= H243), R315 (= R314), E332 (= E331), R334 (= R333)
binding adenosine-5'-diphosphate: K43 (= K43), M50 (= M50), F198 (= F197), Y200 (= Y199), N246 (= N245), S248 (= S247), S324 (≠ K323), S328 (= S327), R330 (= R329)
binding glutamic acid: E133 (= E132), E188 (= E187), V189 (= V188), N239 (= N238), G240 (= G239), G242 (= G241), E303 (= E302)
binding magnesium ion: E131 (= E130), E188 (= E187), E195 (= E194), H244 (= H243), E332 (= E331)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
67% identity, 100% coverage: 3:442/442 of query aligns to 3:443/443 of 4lniA

query
sites
4lniA

Y

Y

T

T

K

R

E

E

D

D

V

I

L

E

R

K

M

L

A

V

R

K

E

E

F

E

D

N

V

V

K

K

F

Y

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

L

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

I

P

P

V

V

D

S

Q

Q

L

L

Q

G

K

K

A

A

L

L

D

D

G

N

E

K

M

V

M

M

F

F

D
|
D

G

G

S

S

I

I

D

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

Y

P

P

D

D

P

L

N

N

T

T

F

F

V

V

I

I

F

F

P

P

W

W

R

T

P

A

Q

E

D

K

G

G

A

K

V

V

A

A

R

R

L

F

I

I

C

C

D

D

V

I

Y

Y

N

N

P

P

D

D

G

G

T

T

P

P

F

F

A

E

G

G

D

D

P

P

R

R

N

N

V

N

L

L

K

K

S

R

V

I

L

L

A

K

E

E

A

M

A

E

E

D

M

L

G

G

Y

F

T

S

-

D

M

F

N

N

V

L

G

G

P

P

E
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E

L

P

E
|
E

F

F

L

L

F

F

L

K

T

L

D

D

A

E

E

K

G

G

K

E

P

P

T

T

T

L

I

E

T

L

H

N

D

D

K

K

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

I

E
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E

A

A

S

S

H

H

E
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E

V

V

A

A

A

P

G

G

Q

Q

H

H

E
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E

I

I

D
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D

F

F

K

K

Y
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Y

S

A

D

G

A

A

L

V

D

R

V

S

A

C

D

D

K

D

I

I

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T

F

F

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K

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L

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I

A

A

Q

R

R

K

H

H

G

G

L

L

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H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G

G

I

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N

N

G

G

S

S

G

G

M

M

H
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H

T

C

N
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N

M

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S
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S

L

L

F

F

K

K

D

N

G

G

K

V

N

N

A

A

F

F

Y

F

D

D

P

E

T

N

T

A

P

D

D

L

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Q

L

L

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S

E

E

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T

A

A

K

K

Y

H

Y

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I

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K

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S

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F

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A

A

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V

T

T

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N

P

P

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V

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N

S

S

Y

Y

K

K

R
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R

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L

V

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P

G

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E
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E

A

A

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P

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C

Y

Y

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V

A

A

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C

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R

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N

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R
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R

S

S

P

P

L

L

I

I

R
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R

I

I

P

P

A

A

K

S

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R

G

G

A

I

S

S

T

T

R
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R

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V

E
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E

L

V

R
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R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

V

V

C

L

L

L

K

A

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

N

K

K

I

L

M

E

P

A

P

P

A

A

P

P

V

I

D

D

E

R

N

N

I

I

Y

Y

H

V

M

M

D

S

A

K

A

E

R

E

R

R

A

M

E

E

I

N

G

G

I

I

V

V

S

D

L

L

P

P

A

A

N

T

L

L

A

A

E

E

A

A

I

L

D

E

E

E

L

F

E

K

K

S

S

N

E

E

V

V

I

M

K

V

E

K

A

A

L

L

G

G

P

E

H

H

V

L

S

F

E

E

R

H

Y

F

I

I

E

E

A

A

K

K

R

E

E

I

E

E

W

W

D

D

S

M

F

F

R

R

I

T

T

Q

V

V

H

H

P

P

W

W

E

E

V

R

E

E

E

Q

Y

Y

L

M

T

S

K

Q

Y

Y

active site: D52 (= D52), E131 (= E130), E133 (= E132), E188 (= E187), E195 (= E194), H244 (= H243), R315 (= R314), E332 (= E331), R334 (= R333)
binding adenosine-5'-diphosphate: E131 (= E130), E183 (= E182), D197 (= D196), Y200 (= Y199), N246 (= N245), S248 (= S247), R320 (= R319), R330 (= R329)
binding magnesium ion: E131 (= E130), E131 (= E130), E133 (= E132), E188 (= E187), E195 (= E194), E195 (= E194), H244 (= H243), E332 (= E331)
binding l-methionine-s-sulfoximine phosphate: E133 (= E132), E188 (= E187), H244 (= H243), R297 (= R296), E303 (= E302), R315 (= R314), R334 (= R333)

4s0rD Structure of gs-tnra complex (see paper)
67% identity, 100% coverage: 3:442/442 of query aligns to 7:447/447 of 4s0rD

query
sites
4s0rD

Y

Y

T

T

K

R

E

E

D

D

V

I

L

E

R

K

M

L

A

V

R

K

E

E

F

E

D

N

V

V

K

K

F

Y

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

L

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

I

P

P

V

V

D

S

Q

Q

L

L

Q

G

K

K

A

A

L

L

D

D

G

N

E

K

M

V

M

M

F

F

D
|
D

G

G

S

S

I

I

D

E

G

G

F
|
F

V
|
V

R
|
R

I
|
I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

Y

P

P

D

D

P

L

N

N

T

T

F

F

V

V

I

I

F

F

P

P

W

W

R

T

P

A

Q

E

D

K

G

G

A

K

V

V

A

A

R

R

L

F

I

I

C

C

D

D

V

I

Y

Y

N

N

P

P

D

D

G

G

T

T

P

P

F

F

A

E

G

G

D

D

P

P

R

R

N

N

V

N

L

L

K

K

S

R

V

I

L

L

A

K

E

E

A

M

A

E

E

D

M

L

G

G

Y

F

T

S

-

D

M

F

N

N

V

L

G

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

L

K

T

L

D

D

A

E

E

K

G

G

K

E

P

P

T

T

T

L

I

E

T

L

H

N

D

D

K

K

A

G

G

G

Y

Y

F

F

D
|
D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

I

E

E

A

A

S

S

H

H

E
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E

V

V

A

A

A

P

G

G

Q

Q

H

H

E
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E

I

I

D

D

F

F

K

K

Y

Y

S

A

D

G

A

A

L

V

D

R

V

S

A

C

D

D

K

D

I

I

V

Q

T

T

F

F

K

K

F

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G
|
G

I
x
V

N
|
N

G

G

S

S

G

G

M

M

H
|
H

T

C

N

N

M

L

S

S

L

L

F

F

K

K

D

N

G

G

K

V

N

N

A

A

F

F

Y

F

D

D

P

E

T

N

T

A

P

D

D

L

Q

Q

L

L

S

S

E

E

T

T

A

A

K

K

Y

H

Y

F

I

I

G

A

G

G

L

I

L

V

K

K

N

H

V

A

R

T

S

S

F

F

A

T

A

A

I

V

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G
|
G

Y
|
Y

E
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E

A

A

P

P

C

C

Y

Y

V

V

A

A

W

W

S

S

C

A

R

Q

N

N

R
|
R

S

S

P

P

L

L

I

I

R

R

I

I

P

P

A

A

K

S

R

R

G

G

A

I

S

S

T

T

R

R

V

V

E
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E

L

V

R
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R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

V

V

C

L

L

L

K

A

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

N

K

K

I

L

M

E

P

A

P

P

A

A

P

P

V

I

D

D

E

R

N

N

I

I

Y
|
Y

H

V

M

M

D

S

A

K

A

E

R

E

R

R

A

M

E

E

I

N

G

G

I

I

V

V

S

D

L

L

P

P

A

A

N

T

L

L

A

A

E

E

A

A

I

L

D

E

E

E

L

F

E

K

K

S

S

N

E

E

V

V

I

M

K

V

E

K

A

A

L

L

G

G

P

E

H

H

V

L

S

F

E

E

R
x
H

Y

F

I

I

E

E

A

A

K

K

R

E

E
x
I

E

E

W

W

D

D

S
x
M

F

F

R

R

I

T

T

Q

V

V

H

H

P

P

W

W

E

E

V

R

E

E

E

Q

Y

Y

L

M

T

S

K

Q

Y

Y

active site: D56 (= D52), E135 (= E130), E137 (= E132), E192 (= E187), E199 (= E194), H248 (= H243), R319 (= R314), E336 (= E331), R338 (= R333)
binding glutamine: E137 (= E132), E192 (= E187), R301 (= R296), E307 (= E302)
binding magnesium ion: I66 (= I62), E135 (= E130), E135 (= E130), E199 (= E194), H248 (= H243), H248 (= H243), E336 (= E331), H419 (≠ R414)
binding : F63 (= F59), V64 (= V60), R65 (= R61), I66 (= I62), D161 (= D156), G241 (= G236), V242 (≠ I237), N243 (= N238), G305 (= G300), Y306 (= Y301), Y376 (= Y371), I426 (≠ E421), M430 (≠ S425)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
67% identity, 100% coverage: 3:442/442 of query aligns to 4:444/444 of P12425

query
sites
P12425

Y

Y

T

T

K

R

E

E

D

D

V

I

L

E

R

K

M

L

A

V

R

K

E

E

F

E

D

N

V

V

K

K

F

Y

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

L

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

I

P

P

V

V

D

S

Q

Q

L

L

Q

G

K

K

A

A

L

L

D

D

G

N

E

K

M

V

M

M

F

F

D

D

G

G

S

S

I

I

D

E

G
|
G

F

F

V

V

R
|
R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

Y

P

P

D

D

P

L

N

N

T

T

F

F

V

V

I

I

F

F

P

P

W

W

R

T

P

A

Q

E

D

K

G

G

A

K

V

V

A

A

R

R

L

F

I

I

C

C

D

D

V

I

Y

Y

N

N

P

P

D

D

G

G

T

T

P

P

F

F

A

E

G

G

D

D

P

P

R

R

N

N

V

N

L

L

K

K

S

R

V

I

L

L

A

K

E

E

A

M

A

E

E

D

M

L

G

G

Y

F

T

S

-

D

M

F

N

N

V

L

G

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

L

K

T

L

D

D

A

E

E

K

G

G

K

E

P

P

T

T

T

L

I

E

T

L

H

N

D

D

K

K

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

I

E

E

A

A

S

S

H

H

E
|
E

V
|
V

A

A

A

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

A

D

G

A

A

L

V

D

R

V

S

A

C

D

D

K

D

I

I

V

Q

T

T

F

F

K

K

F

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G

G

I

V

N

N

G
|
G

S

S

G

G

M

M

H
|
H

T

C

N

N

M

L

S

S

L

L

F

F

K

K

D

N

G

G

K

V

N

N

A

A

F

F

Y

F

D

D

P

E

T

N

T

A

P

D

D

L

Q

Q

L

L

S

S

E

E

T

T

A

A

K

K

Y

H

Y

F

I

I

G

A

G

G

L

I

L

V

K

K

N

H

V

A

R

T

S

S

F

F

A

T

A

A

I

V

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G
|
G

Y

Y

E
|
E

A

A

P
|
P

C

C

Y

Y

V

V

A

A

W

W

S

S

C

A

R

Q

N

N

R

R

S

S

P

P

L

L

I

I

R

R

I

I

P

P

A

A

K

S

R

R

G

G

A

I

S

S

T

T

R

R

V

V

E
|
E

L

V

R

R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

V

V

C

L

L

L

K

A

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

N

K

K

I

L

M

E

P

A

P

P

A

A

P

P

V

I

D

D

E

R

N

N

I

I

Y

Y

H

V

M

M

D

S

A

K

A

E

R

E

R

R

A

M

E

E

I

N

G

G

I

I

V

V

S

D

L

L

P

P

A

A

N

T

L

L

A

A

E

E

A

A

I

L

D

E

E

E

L

F

E

K

K

S

S

N

E

E

V

V

I

M

K

V

E

K

A

A

L

L

G

G

P

E

H

H

V

L

S

F

E

E

R

H

Y

F

I

I

E

E

A

A

K

K

R

E

E

I

E
|
E

W

W

D

D

S

M

F

F

R

R

I

T

T

Q

V

V

H

H

P

P

W

W

E

E

V

R

E

E

E

Q

Y

Y

L

M

T

S

K

Q

Y

Y

G59 (= G58) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (= R61) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E130) binding Mg(2+)
E134 (= E132) binding Mg(2+)
E189 (= E187) binding Mg(2+)
V190 (= V188) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E194) binding Mg(2+)
G241 (= G239) binding L-glutamate
H245 (= H243) binding Mg(2+)
G302 (= G300) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (= E302) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P304) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E331) binding Mg(2+)
E424 (= E422) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7tfaB Glutamine synthetase (see paper)
68% identity, 100% coverage: 2:442/442 of query aligns to 1:441/441 of 7tfaB

query
sites
7tfaB

G

S

Y

Y

T

T

K

R

E

E

D

D

V

I

L

I

R

R

M

I

A

A

R

E

E

E

F

E

D

N

V

V

K

R

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

L

L

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

I

P

P

V

V

D

S

Q

Q

L

L

Q

E

K

K

A

A

L

L

D

D

G

N

E

K

M

M

F

F

D

D

G

G

S

S

I

I

D

E

G

G

F
x
Y

V

V

R
|
R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

Y

P

P

D

D

P

L

N

D

T

T

F

W

V

V

I

V

F

F

P

P

W

W

R

V

P

T

Q

S

D

D

G

-

A

R

V

V

A

A

R

R

L

L

I

I

C

C

D

D

V

I

Y

Y

N

K

P

P

D

D

G

G

T

S

P

P

F

F

A

A

G

G

D

D

P

P

R

R

N

G

V

I

L

L

K

K

S

R

V

V

L

L

A

K

E

E

A

A

A

E

E

E

M

L

G

G

Y

Y

T

T

-

S

M

M

N

N

V

V

G

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

L

K

T

T

D

D

A

E

E

K

G

G

K

D

P

P

T

T

I

E

T

L

H

N

D

D

K

Q

A

G

G

G

Y
|
Y

F

F

D

D

M

L

T

A

P

P

I

M

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

E

M

I

V

V

L

L

T

K

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

I

E

E

A

A

S

S

H

H

E
|
E

V
|
V

A

A

A

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

A

D

D

A

A

L

V

D

K

V

A

A

A

D

D

K

Q

I

I

V

Q

T

T

F

F

K

K

F

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

Q

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G

G

I

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

T

C

N

N

M

Q

S

S

L

L

F

F

K

K

D

D

G

N

K

E

N

N

A

V

F

F

Y

Y

D

D

P

E

T

T

T

D

P

E

D

L

Q

G

L

L

S

S

E

Q

T

T

A

A

K

R

Y

H

Y

Y

I

M

G

A

G

G

L

I

L

L

K

K

N

H

V

A

R

R

S

A

F

M

A

A

I

I

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G
|
G

Y
|
Y

E
|
E

A

A

P

P

C

C

Y

Y

V

V

A

A

W

W

S

S

C

A

R

S

N

N

R
|
R

S

S

P

P

L

M

I

I

R

R

I

I

P

P

A

A

K

S

R

R

G

G

A

L

S

S

T

T

R

R

V

V

E
|
E

L

V

R

R

S

N

P

P

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

A

V

V

C

M

L

L

K

R

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

R

K

Q

I

M

M

A

P

L

P

P

A

A

P

P

V

I

D

D

E

R

N

N

I

I

Y

Y

H

V

M

M

D

S

A

E

R

E

R

R

A

I

E

E

I

E

G

G

I

I

V

P

S

S

L

L

P

P

A

A

N

D

L

L

A

K

E

E

A

A

I

L

D

S

E

E

L

L

E

I

K

R

S

S

E

E

V

V

I

I

K

S

E

D

A

A

L

L

G

G

P

D

H

H

V

A

S

L

E

A

R

Y

Y

F

I

Y

E

E

A

L

K

K

R

E

E

I

E

E

W

W

D

D

S
x
M

F

Y

R

R

I

T

T

Q

V

V

H

H

P

Q

W

W

E

E

V

R

E

D

E

Q

Y

Y

L

L

T

T

K

L

Y

Y

binding glutamine: E131 (= E132), Y153 (= Y154), E186 (= E187), G238 (= G239), H242 (= H243), R295 (= R296), E301 (= E302)
binding magnesium ion: E129 (= E130), E131 (= E132), E186 (= E187), E193 (= E194), H242 (= H243), E330 (= E331)
binding : Y58 (≠ F59), R60 (= R61), V187 (= V188), N237 (= N238), G299 (= G300), Y300 (= Y301), R313 (= R314), M424 (≠ S425)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
67% identity, 100% coverage: 2:442/442 of query aligns to 1:439/439 of 7tdpA

query
sites
7tdpA

G

S

Y

Y

T

T

K

R

E

E

D

D

V

I

L

I

R

R

M

I

A

A

R

E

E

E

F

E

D

N

V

V

K

R

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

L

L

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

I

P

P

V

V

D

S

Q

Q

L

L

Q

E

K

K

A

A

L

L

D

D

G

N

E

K

M

M

F

F

D

D

G

G

S

S

I

I

D

E

G

G

F

Y

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

Y

P

P

D

D

P

L

N

D

T

T

F

W

V

V

I

V

F

F

P

P

W

W

R

S

P

D

Q

R

D

-

G

-

A

-

V

V

A

A

R

R

L

L

I

I

C

C

D

D

V

I

Y

Y

N

K

P

P

D

D

G

G

T

S

P

P

F

F

A

A

G

G

D

D

P

P

R

R

N

G

V

I

L

L

K

K

S

R

V

V

L

L

A

K

E

E

A

A

A

E

E

E

M

L

G

G

Y

Y

T

T

-

S

M

M

N
|
N

V

V

G
|
G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

L

K

T

T

D

D

A

E

E

K

G

G

K

D

P

P

T

T

I

E

T

L

H

N

D

D

K

Q

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

M

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

E

M

I

V

V

L

L

T

K

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

I

E
|
E

A

A

S

S

H

H

E
|
E

V

V

A

A

A

P

G

G

Q

Q

H

H

E
|
E

I

I

D
|
D

F

F

K

K

Y
|
Y

S

A

D

D

A

A

L

V

D

K

V

A

A

A

D

D

K

Q

I

I

V

Q

T

T

F

F

K

K

F

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

Q

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G

G

I

V

N

N

G
|
G

S

S

G

G

M

M

H
|
H

T

C

N
|
N

M

Q

S
|
S

L

L

F

F

K

K

D

D

G

N

K

E

N

N

A

V

F

F

Y

Y

D

D

P

E

T

T

T

D

P

E

D

L

Q

G

L

L

S

S

E

Q

T

T

A

A

K

R

Y

H

Y

Y

I

M

G

A

G

G

L

I

L

L

K

K

N

H

V

A

R

R

S

A

F

M

A

A

I

I

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

C

C

Y

Y

V

V

A

A

W

W

S

S

C

A

R

S

N

N

R
|
R

S

S

P

P

L

M

I

I

R
|
R

I

I

P

P

A

A

K

S

R

R

G

G

A

L

S

S

T

T

R
|
R

V

V

E
|
E

L

V

R
|
R

S

N

P

P

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

A

V

V

C

M

L

L

K

R

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

R

K

Q

I

M

M

A

P

L

P

P

A

A

P

P

V

I

D

D

E

R

N

N

I

I

Y

Y

H

V

M

M

D

S

A

E

R

E

R

R

A

I

E

E

I

E

G

G

I

I

V

P

S

S

L

L

P

P

A

A

N

D

L

L

A

K

E

E

A

A

I

L

D

S

E

E

L

L

E

I

K

R

S

S

E

E

V

V

I

I

K

S

E

D

A

A

L

L

G

G

P

D

H

H

V

A

S

L

E

A

R

Y

Y

F

I

Y

E

E

A

L

K

K

R

E

E

I

E

E

W

W

D

D

S

M

F

Y

R

R

I

T

T

Q

V

V

H

H

P

Q

W

W

E

E

V

R

E

D

E

Q

Y

Y

L

L

T

T

K

L

Y

Y

binding adenosine-5'-diphosphate: N123 (= N126), G125 (= G128), E127 (= E130), E179 (= E182), D193 (= D196), Y196 (= Y199), N242 (= N245), S244 (= S247), R316 (= R319), R326 (= R329)
binding magnesium ion: E127 (= E130), E127 (= E130), E129 (= E132), E184 (= E187), E191 (= E194), E191 (= E194), H240 (= H243), E328 (= E331)
binding l-methionine-s-sulfoximine phosphate: E127 (= E130), E129 (= E132), E184 (= E187), E191 (= E194), G236 (= G239), H240 (= H243), R293 (= R296), E299 (= E302), R311 (= R314), R330 (= R333)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
65% identity, 100% coverage: 3:442/442 of query aligns to 3:443/443 of 7tf9S

query
sites
7tf9S

Y

Y

T

T

K

K

E

E

D

D

V

I

L

F

R

R

M

F

A

A

R

D

E

E

F

Q

D

N

V

V

K

K

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

L

L

G

G

V

I

L

I

K

K

N

N

V

V

A

E

I

I

P

P

V

V

D

S

Q

Q

L

L

Q

K

K

K

A

A

L

L

D

D

G

N

E

K

M

I

M

M

F

F

D

D

G

G

S

S

I

I

D

E

G

G

F
|
F

V
|
V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

F

P

P

D

D

P

L

N

D

T

T

F

W

V

V

I

V

F

F

P

P

W

W

R

T

P

A

Q

E

D

K

G

G

A

K

V

V

A

A

R

R

L

M

I

I

C

C

D

D

V

I

Y

Y

N

N

P

P

D

D

G

M

T

T

P

P

F

F

A

A

G

G

D

D

P

P

R

R

N

A

V

N

L

L

K

K

S

R

V

V

L

L

A

K

E

E

A

M

A

E

E

E

M

L

G

G

Y

F

T

T

-

E

M

F

N

N

V

L

G

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

L

K

T

L

D

D

A

E

E

N

G

R

K

R

P

P

T

T

T

L

I

E

T

L

H

N

D

D

K

S

A

G

G

G

Y
|
Y

F

F

D

D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

A

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

E

D

D

A

A

L

I

D

T

V

A

A

C

D

D

K

S

I

I

V

Q

T

T

F

F

K

K

F

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G

G

I

V

N

N

G
|
G

S

S

G
|
G

M

M

H
|
H

T

F

N

N

M

M

S

S

L

L

F

F

K

N

D

E

G

K

K

G

N

N

A

A

F

F

Y

F

D

D

P

E

T

S

T

G

P

E

D

L

Q

E

L

L

S

S

E

Q

T

T

A

A

K

Y

Y

H

Y

F

I

L

G

A

G

G

L

M

L

L

K

K

N

H

V

A

R

R

S

G

F

Y

A

T

A

A

I

V

T

T

N

N

P

P

T

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

C

C

Y

Y

V

I

A

A

W

W

S

S

C

G

R

K

N

N

R

R

S

S

P

P

L

L

I

V

R

R

I

V

P

P

A

S

K

S

R

R

G

G

A

L

S

S

T

T

R

R

V

L

E
|
E

L

L

R

R

S

S

P

V

D

D

P

P

S

S

C

A

N

N

P

P

Y

Y

L

L

A

A

L

M

A

A

V

V

C

L

L

L

K

K

A

A

G

G

L

L

D

S

G

G

I

I

K

K

N

D

K

E

I

L

M

T

P

P

A

A

P

P

V

V

D

D

E

R

N

N

I

I

Y

Y

H

G

M

M

D

N

A

E

R

E

R

R

A

E

E

A

I

T

G

G

I

I

V

Y

S

D

L

L

P

P

A

E

N

S

L

L

A

G

E

H

A

A

I

L

D

I

E

E

L

L

E

E

K

K

S

N

E

E

V

I

I

I

K

K

E

D

A

G

L

L

G

G

P

E

H

H

V

I

S

F

E

E

R

H

Y

F

I

I

E
|
E

A

A

K

K

R

T

E
x
I

E

E

W

C

D

D

S
x
M

F

F

R

R

I

T

T

A

V

V

H

H

P

P

W

W

E

E

V

R

E

E

E

Q

Y

Y

L

L

T

E

K

I

Y

Y

binding glutamine: E133 (= E132), Y155 (= Y154), E188 (= E187), G240 (= G239), G242 (= G241), R297 (= R296), E303 (= E302)
binding magnesium ion: E131 (= E130), E133 (= E132), E188 (= E187), E195 (= E194), H244 (= H243), E332 (= E331)
binding : F59 (= F59), V60 (= V60), E418 (= E417), I422 (≠ E421), M426 (≠ S425)

7tenA Glutamine synthetase (see paper)
65% identity, 100% coverage: 3:442/442 of query aligns to 2:442/442 of 7tenA

query
sites
7tenA

Y

Y

T

T

K

K

E

E

D

D

V

I

L

F

R

R

M

F

A

A

R

D

E

E

F

Q

D

N

V

V

K

K

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

L

L

G

G

V

I

L

I

K

K

N

N

V

V

A

E

I

I

P

P

V

V

D

S

Q

Q

L

L

Q

K

K

K

A

A

L

L

D

D

G

N

E

K

M

I

M

M

F

F

D

D

G

G

S

S

I

I

D

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

F

P

P

D

D

P

L

N

D

T

T

F

W

V

V

I

V

F

F

P

P

W

W

R

T

P

A

Q

E

D

K

G

G

A

K

V

V

A

A

R

R

L

M

I

I

C

C

D

D

V

I

Y

Y

N

N

P

P

D

D

G

M

T

T

P

P

F

F

A

A

G

G

D

D

P

P

R

R

N

A

V

N

L

L

K

K

S

R

V

V

L

L

A

K

E

E

A

M

A

E

E

E

M

L

G

G

Y

F

T

T

-

E

M

F

N

N

V

L

G
|
G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

L

K

T

L

D

D

A

E

E

N

G

R

K

R

P

P

T

T

T

L

I

E

T

L

H

N

D

D

K

S

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

I

E
|
E

A

A

S

S

H

H

E
|
E

V

V

A

A

A

P

G

G

Q

Q

H

H

E
|
E

I

I

D
|
D

F
|
F

K
|
K

Y
|
Y

S

E

D

D

A

A

L

I

D

T

V

A

A

C

D

D

K

S

I

I

V

Q

T

T

F

F

K

K

F

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G

G

I

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

T

F

N
|
N

M

M

S
|
S

L

L

F

F

K

N

D

E

G

K

K

G

N

N

A

A

F

F

Y

F

D

D

P

E

T

S

T

G

P

E

D

L

Q

E

L

L

S

S

E

Q

T

T

A

A

K

Y

Y

H

Y

F

I

L

G

A

G

G

L

M

L

L

K

K

N

H

V

A

R

R

S

G

F

Y

A

T

A

A

I

V

T

T

N

N

P

P

T

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

C

C

Y

Y

V

I

A

A

W

W

S

S

C

G

R

K

N

N

R
|
R

S

S

P

P

L

L

I

V

R
|
R

I

V

P

P

A

S

K

S

R

R

G

G

A

L

S
|
S

T

T

R
|
R

V

L

E

E

L

L

R
|
R

S

S

P

V

D

D

P

P

S

S

C

A

N

N

P

P

Y

Y

L

L

A

A

L

M

A

A

V

V

C

L

L

L

K

K

A

A

G

G

L

L

D

S

G

G

I

I

K

K

N

D

K

E

I

L

M

T

P

P

A

A

P

P

V

V

D

D

E

R

N

N

I

I

Y

Y

H

G

M

M

D

N

A

E

R

E

R

R

A

E

E

A

I

T

G

G

I

I

V

Y

S

D

L

L

P

P

A

E

N

S

L

L

A

G

E

H

A

A

I

L

D

I

E

E

L

L

E

E

K

K

S

N

E

E

V

I

I

I

K

K

E

D

A

G

L

L

G

G

P

E

H

H

V

I

S

F

E

E

R

H

Y

F

I

I

E

E

A

A

K

K

R

T

E

I

E

E

W

C

D

D

S

M

F

F

R

R

I

T

T

A

V

V

H

H

P

P

W

W

E

E

V

R

E

E

E

Q

Y

Y

L

L

T

E

K

I

Y

Y

binding adenosine-5'-diphosphate: G128 (= G128), E130 (= E130), E182 (= E182), D196 (= D196), F197 (= F197), K198 (= K198), Y199 (= Y199), N245 (= N245), S247 (= S247), R319 (= R319), S327 (= S327), R329 (= R329)
binding l-methionine-s-sulfoximine phosphate: E130 (= E130), E132 (= E132), E187 (= E187), E194 (= E194), N238 (= N238), G239 (= G239), H243 (= H243), R296 (= R296), E302 (= E302), R314 (= R314), R333 (= R333)

8ufjB Glutamine synthetase (see paper)
64% identity, 99% coverage: 4:440/442 of query aligns to 6:442/444 of 8ufjB

query
sites
8ufjB

T

T

K

K

E

E

D

D

V

V

L

L

R

E

M

A

A

V

R

K

E

E

F

R

D

D

V

V

K

K

F

F

I

I

R

R

L

T

Q

Q

F

F

T

T

D

D

I

T

L

L

G

G

V

I

L

I

K

K

N

S

V

W

A

A

I

I

P

P

V

A

D

E

Q

Q

L

L

Q

E

K

E

A

A

L

F

D

E

G

N

E

G

M

V

M

M

F

F

D

D

G

G

S

S

I

I

D

Q

G

G

F

F

V

T

R

R

I

I

E

E

S

S

D

D

M

M

Y

K

L

L

R

A

P

L

D

D

P

P

N

S

T

T

F

F

V

R

I

I

F

L

P

P

W

W

R

R

P

P

Q

A

D

T

G

G

A

A

V

V

A

A

R

R

L

I

I

L

C

G

D

D

V

V

Y

Y

N

L

P

P

D

D

G

G

T

N

P

P

F

F

A

K

G

G

D

D

P

P

R

R

N

Y

V

V

L

L

K

K

S

T

V

A

L

I

A

K

E

E

A

A

A

E

E

K

M

M

G

G

Y

F

T

S

M

M

N

N

V

V

G

G

P

P

E
|
E

L

L

E
|
E

F

F

L

L

F

F

L

K

T

L

D

D

A

A

E

N

G

G

K

N

P

P

T

T

I

E

T

L

H

T

D

D

K

Q

A

G

G

G

Y

Y

F

F

D

D

M

F

T

A

P

P

I

L

D

D

L

L

G

G

E

Q

N

D

A

V

R

R

D

D

M

I

V

D

L

Y

T

A

L

L

E

E

Q

H

M

M

G

G

F

F

E

Q

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

A

P

G

S

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

R

Y

F

S

G

D

D

A

V

L

L

D

C

V

T

A

A

D

D

K

N

I

V

V

V

T

T

F

F

K

K

F

Y

V

V

R

K

T

S

I

I

A

A

Q

Y

R

H

H

K

G

G

L

Y

H

Y

A

A

T

S

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G

G

I

V

N

N

G

G

S

S

G

G

M

M

H
|
H

T

S

N

N

M

Q

S

S

L

L

F

F

K

K

D

D

G

G

K

K

N

N

A

V

F

F

Y

Y

D

D

P

P

T

D

T

T

P

P

D

T

Q

K

L

L

S

S

E

Q

T

D

A

A

K

M

Y

Y

I

I

G

G

L

L

K

K

N

H

V

I

R

R

S

E

F

F

A

T

A

A

I

V

T

T

N

N

P

P

T

V

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

Y

Y

E

E

A

A

P

P

C

V

Y

Y

V

I

A

S

W

W

S

S

C

A

R

Q

N

N

R

R

S

S

P

S

L

L

I

I

R

R

I

I

P

P

A

A

K

T

R

R

G

G

A

N

S

G

T

T

R

R

V

I

E
|
E

L

L

R

R

S

C

P

P

D

D

P

P

S

A

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

V

L

C

M

L

L

K

R

A

A

G

G

L

L

D

E

G

G

I

I

K

K

N

N

K

K

I

I

M

D

P

P

P

G

A

E

P

P

V

T

D

N

E

V

N

N

I

I

Y

F

H

H

M

L

D

S

A

D

A

K

R

E

R

R

A

E

E

E

I

R

G

G

I

I

V

R

S

S

L

L

P

P

A

A

N

D

L

L

A

K

E

E

A

A

I

I

D

D

E

E

L

M

E

K

K

G

S

S

E

K

V

F

I

V

K

K

E

E

A

A

L

L

G

G

P

E

H

H

V

V

S

F

E

S

R

H

Y

Y

I

L

E

C

A

A

K

K

R

E

E

M

E

E

W

W

D

D

S

E

F

Y

R

K

I

A

T

V

V

V

H

H

P

P

W

W

E

E

V

L

E

S

E

R

Y

Y

L

L

T

S

binding magnesium ion: E132 (= E130), E134 (= E132), E189 (= E187), E196 (= E194), H245 (= H243), E333 (= E331)

8tfkA Glutamine synthetase (see paper)
63% identity, 99% coverage: 4:440/442 of query aligns to 2:438/440 of 8tfkA

query
sites
8tfkA

T

T

K

K

E

E

D

D

V

V

L

L

R

E

M

A

A

V

R

K

E

E

F

R

D

D

V

V

K

K

F

F

I

I

R

R

L

T

Q

Q

F

F

T

T

D

D

I

T

L

L

G

G

V

I

L

I

K

K

N

S

V

W

A

A

I

I

P

P

V

A

D

E

Q

Q

L

L

Q

E

K

E

A

A

L

F

D

E

G

N

E

G

M

V

M

M

F

F

D

D

G

G

S

S

I

I

D

Q

G

G

F

F

V

T

R

R

I

I

E

E

S

S

D

D

M

M

Y

K

L

L

R

A

P

L

D

D

P

P

N

S

T

T

F

F

V

R

I

I

F

L

P

P

W

W

R

R

P

P

Q

A

D

T

G

G

A

A

V

V

A

A

R

R

L

I

I

L

C

G

D

D

V

V

Y

Y

N

L

P

P

D

D

G

G

T

N

P

P

F

F

A

K

G

G

D

D

P

P

R

R

N

Y

V

V

L

L

K

K

S

T

V

A

L

I

A

K

E

E

A

A

A

E

E

K

M

M

G

G

Y

F

T

S

M

M

N

N

V

V

G

G

P

P

E
|
E

L

L

E
|
E

F

F

L

L

F

F

L

K

T

L

D

D

A

A

E

N

G

G

K

N

P

P

T

T

I

E

T

L

H

T

D

D

K

Q

A

G

G

G

Y

Y

F

F

D

D

M

F

T

A

P

P

I

L

D

D

L

R

G

A

E

Q

N

D

A

V

R

R

D

D

M

I

V

D

L

Y

T

A

L

L

E

E

Q

H

M

M

G

G

F

F

E

Q

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

A

P

G

S

Q

Q

H

H

E
|
E

I

I

D
|
D

F
|
F

K

R

Y
x
F

S

G

D

D

A

V

L

L

D

C

V

T

A

A

D

D

K

N

I

V

V

V

T

T

F

F

K

K

F

Y

V

V

R

K

T

S

I

I

A

A

Q

Y

R

H

H

K

G

G

L

Y

H

Y

A

A

T

S

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G

G

I

V

N

N

G
|
G

S

S

G

G

M

M

H
|
H

T

S

N
|
N

M

Q

S

S

L

L

F

F

K

K

D

D

G

G

K

K

N

N

A

V

F

F

Y

Y

D

D

P

P

T

D

T

T

P

P

D

T

Q

K

L

L

S

S

E

Q

T

D

A

A

K

M

Y

Y

I

I

G

G

L

L

K

K

N

H

V

I

R

R

S

E

F

F

A

T

A

A

I

V

T

T

N

N

P

P

T

V

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

C

V

Y

Y

V

I

A

S

W

W

S

S

C

A

R

Q

N

N

R
|
R

S

S

P

S

L

L

I

I

R
|
R

I

I

P

P

A

A

K

T

R

R

G

G

A

N

S
x
G

T

T

R
|
R

V

I

E
|
E

L

L

R
|
R

S

C

P

P

D

D

P

P

S

A

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

V

L

C

M

L

L

K

R

A

A

G

G

L

L

D

E

G

G

I

I

K

K

N

N

K

K

I

I

M

D

P

P

P

G

A

E

P

P

V

T

D

N

E

V

N

N

I

I

Y

F

H

H

M

L

D

S

A

D

A

K

R

E

R

R

A

E

E

E

I

R

G

G

I

I

V

R

S

S

L

L

P

P

A

A

N

D

L

L

A

K

E

E

A

A

I

I

D

D

E

E

L

M

E

K

K

G

S

S

E

K

V

F

I

V

K

K

E

E

A

A

L

L

G

G

P

E

H

H

V

V

S

F

E

S

R

H

Y

Y

I

L

E

C

A

A

K

K

R

E

E

M

E

E

W

W

D

D

S

E

F

Y

R

K

I

A

T

V

V

V

H

H

P

P

W

W

E

E

V

L

E

S

E

R

Y

Y

L

L

T

S

binding adenosine-5'-diphosphate: E128 (= E130), D194 (= D196), F195 (= F197), F197 (≠ Y199), N243 (= N245), R312 (= R314), R317 (= R319), G325 (≠ S327), R327 (= R329)
binding magnesium ion: E128 (= E130), E128 (= E130), E130 (= E132), E185 (= E187), E192 (= E194), E192 (= E194), H241 (= H243), E329 (= E331)
binding l-methionine-s-sulfoximine phosphate: E128 (= E130), E130 (= E132), E185 (= E187), E192 (= E194), G237 (= G239), H241 (= H243), R294 (= R296), E300 (= E302), R312 (= R314), R331 (= R333)

7tdvC Glutamine synthetase (see paper)
62% identity, 100% coverage: 3:442/442 of query aligns to 3:443/443 of 7tdvC

query
sites
7tdvC

Y

F

T

T

K

K

E

E

D

D

V

I

L

R

R

K

M

F

A

V

R

E

E

E

F

E

D

N

V

V

K

R

F

Y

I

L

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

L

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

V

P

P

V

V

D

S

Q

Q

L

L

Q

E

K

K

A

V

L

L

D

D

G

N

E

E

M

M

F

F

D

D

G

G

S

S

I

I

D

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

H

P

P

D

D

P

L

N

D

T

T

F

W

V

V

I

I

F

F

P

P

W

W

R

T

P

A

Q

G

D

Q

G

G

A

K

V

V

A

A

R

R

L

L

I

I

C

C

D

D

V

V

Y

Y

N

K

P

T

D

D

G

G

T

T

P

P

F

F

A

E

G

G

D

D

P

P

R

R

N

A

V

N

L

L

K

K

S

R

V

V

L

L

A

K

E

E

A

M

A

E

E

D

M

L

G

G

Y

F

T

T

-

D

M

F

N

N

V

L

G
|
G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

L

K

T

L

D

D

A

E

E

K

G

G

K

E

P

P

T

T

T

L

I

E

T

L

H

N

D

D

K

D

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

D

M

M

G

G

F

F

E

D

I

I

E
|
E

A

A

S

S

H

H

E
|
E

V

V

A

A

A

P

G

G

Q

Q

H

H

E
|
E

I

I

D
|
D

F
|
F

K
|
K

Y
|
Y

S

A

D

D

A

A

L

V

D

T

V

A

A

C

D

D

K

N

I

I

V

Q

T

T

F

F

K

K

F

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

N

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G

G

I

V

N

N

G
|
G

S

S

G

G

M

M

H
|
H

T

F

N
|
N

M
x
V

S
|
S

L

L

F

F

K

K

D

G

G

K

K

E

N

N

A

A

F

F

Y

F

D

D

P

P

T

N

T

T

P

E

D

M

Q

G

L

L

S

T

E

E

T

T

A

A

K

Y

Y

Q

Y

F

I

T

G

A

G

G

L

V

L

L

K

K

N

N

V

A

R

R

S

G

F

F

A

T

A

A

I

V

T

C

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

C

C

Y

Y

V

I

A

A

W

W

S

S

C

G

R

K

N

N

R
|
R

S

S

P

P

L

L

I

I

R
|
R

I

V

P

P

A

S

K

S

R

R

G

G

A

L

S
|
S

T

T

R
|
R

V

I

E
|
E

L

V

R

R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

M

A

A

L

L

A

A

V

A

C

I

L

L

K

E

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

N

K

K

I

L

M

K

P

V

P

P

A

E

P

P

V

V

D

N

E

Q

N

N

I

I

Y

Y

H

E

M

M

D

N

A

R

A

E

R

E

R

R

A

E

E

A

I

V

G

G

I

I

V

Q

S

D

L

L

P

P

A

S

N

T

L

L

A

Y

E

T

A

A

I

L

D

K

E

A

L

M

E

R

K

E

S

N

E

E

V

V

I

I

K

K

E

K

A

A

L

L

G

G

P

N

H

H

V

I

S

Y

E

N

R

Q

Y

F

I

I

E

N

A

S

K

K

R

S

E

I

E

E

W

W

D

D

S

Y

F

Y

R

R

I

T

T

Q

V

V

H

S

P

E

W

W

E

E

V

R

E

D

E

Q

Y

Y

L

M

T

K

K

Q

Y

Y

binding adenosine-5'-diphosphate: G129 (= G128), E131 (= E130), E183 (= E182), D197 (= D196), F198 (= F197), K199 (= K198), Y200 (= Y199), N246 (= N245), V247 (≠ M246), S248 (= S247), R320 (= R319), S328 (= S327), R330 (= R329)
binding magnesium ion: E131 (= E130), E131 (= E130), E133 (= E132), E188 (= E187), E195 (= E194), E195 (= E194), H244 (= H243), E332 (= E331)
binding l-methionine-s-sulfoximine phosphate: E131 (= E130), E133 (= E132), E188 (= E187), E195 (= E194), G240 (= G239), H244 (= H243), R297 (= R296), E303 (= E302), R315 (= R314)

7tf6A Glutamine synthetase (see paper)
62% identity, 100% coverage: 3:442/442 of query aligns to 2:438/438 of 7tf6A

query
sites
7tf6A

Y

F

T

T

K

K

E

E

D

D

V

I

L

R

R

K

M

F

A

A

R

E

E

E

F

E

D

N

V

V

K

R

F

Y

I

L

R

R

L

L

Q

Q

F

F

T

T

D

D

I

I

L

L

G

G

V

T

L

I

K

K

N

N

V

V

A

E

I

V

P

P

V

V

D

S

Q

Q

L

L

Q

E

K

K

A

V

L

L

D

D

G

N

E

E

M

M

F

F

D

D

G

G

S

S

I

I

D

E

G

G

F
|
F

V

V

R
|
R

I

I

E

E

S

S

D

D

M

M

Y

Y

L

L

R

H

P

P

D

D

P

L

N

D

T

T

F

W

V

V

I

I

F

F

P

P

W

W

R

-

P

-

Q

-

D

-

G

G

A

K

V

V

A

A

R

R

L

L

I

I

C

C

D

D

V

V

Y

Y

N

K

P

T

D

D

G

G

T

T

P

P

F

F

A

E

G

G

D

D

P

P

R

R

N

A

V

N

L

L

K

K

S

R

V

V

L

L

A

K

E

E

A

M

A

E

E

D

M

L

G

G

Y

F

T

T

-

D

M

F

N

N

V

L

G

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

L

K

T

L

D

D

A

E

E

K

G

G

K

E

P

P

T

T

T

L

I

E

T

L

H

N

D

D

K

D

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

T

D

D

L

L

G

G

E

E

N

N

A

C

R

R

D

D

M

I

V

V

L

L

T

E

L

L

E

E

Q

D

M

M

G

G

F

F

E

D

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

A

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

A

D

D

A

A

L

V

D

T

V

A

A

C

D

D

K

N

I

I

V

Q

T

T

F

F

K

K

F

L

V

V

R

K

T

T

I

I

A

A

Q

R

R

K

H

H

G

N

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G
|
G

I

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

T

F

N

N

M

V

S

S

L

L

F

F

K

K

D

G

G

K

K

E

N

N

A

A

F

F

Y

F

D

D

P

P

T

N

T

T

P

E

D

M

Q

G

L

L

S

T

E

E

T

T

A

A

K

Y

Y

Q

Y

F

I

T

G

A

G

G

L

V

L

L

K

K

N

N

V

A

R

R

S

G

F

F

A

T

A

A

I

V

T

C

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G
|
G

Y
|
Y

E
|
E

A

A

P

P

C

C

Y

Y

V

I

A

A

W

W

S

S

C

G

R

K

N

N

R
|
R

S

S

P

P

L

L

I

I

R

R

I

V

P

P

A

S

K

S

R

R

G

G

A

L

S

S

T

T

R

R

V

I

E
|
E

L

V

R

R

S

S

P

V

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

M

A

A

L

L

A

A

V

A

C

I

L

L

K

E

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

N

K

K

I

L

M

K

P

V

P

P

A

E

P

P

V

V

D

N

E

Q

N

N

I

I

Y
|
Y

H

E

M

M

D

N

A

R

A

E

R

E

R

R

A

E

E

A

I

V

G

G

I

I

V

Q

S

D

L

L

P

P

A

S

N

T

L

L

A

Y

E

T

A

A

I

L

D

K

E

A

L

M

E

R

K

E

S

N

E

E

V

V

I

I

K

K

E

K

A

A

L

L

G

G

P

N

H

H

V

I

S

Y

E

N

R

Q

Y

F

I

I

E

N

A

S

K

K

R

S

E

I

E

E

W

W

D

D

S
x
Y

F

Y

R

R

I

T

T

Q

V

V

H

S

P

E

W

W

E

E

V

R

E

D

E
x
Q

Y

Y

L

M

T

K

K
x
Q

Y

Y

binding glutamine: E128 (= E132), E183 (= E187), G235 (= G239), H239 (= H243), R292 (= R296), E298 (= E302)
binding magnesium ion: E126 (= E130), E128 (= E132), E183 (= E187), E190 (= E194), H239 (= H243), E327 (= E331)
binding : F58 (= F59), R60 (= R61), G232 (= G236), N234 (= N238), G296 (= G300), Y297 (= Y301), R310 (= R314), Y367 (= Y371), Y421 (≠ S425), Q433 (≠ E437), Q437 (≠ K441)

8ooxB Glutamine synthetase (see paper)
61% identity, 99% coverage: 4:442/442 of query aligns to 1:438/438 of 8ooxB

query
sites
8ooxB

T

S

K

K

E

E

D

D

-

E

V

I

L

F

R

R

M

I

A

V

R

E

E

E

F

K

D

N

V

V

K

R

F

F

I

V

R

R

L

L

Q

Q

F

F

T

V

D

D

I

V

L

Q

G

G

V

I

L

P

K

K

N

N

V

V

A

A

I

I

P

P

V

V

D

G

Q

Q

L

L

Q

E

K

K

A

A

L

L

D

G

G

P

E

G

M

I

M

H

F

F

D

D

G

G

S

S

I

I

D

E

G

G

F

F

V

V

R

R

I

I

E

E

S

S

D

D

M

M

Y

V

L

L

R

R

P

P

D

D

P

P

N

D

T

T

F

F

V

R

I

V

F

L

P

P

W

W

R

S

P

-

Q

G

D

N

G

T

A

A

V

E

A

A

R

R

L

L

I

I

C

C

D

D

V

I

Y

E

N

L

P

P

D

D

G

G

T

K

P

P

F

F

A

M

G

G

D

C

P

P

R

R

N

Q

V

V

L

L

K

K

S

K

V

N

L

M

A

E

E

E

A

A

E

K

M

L

G

G

Y

Y

T

V

M

M

N

N

V

T

G

G

P

P

E

E

L

M

E
|
E

F

F

L

L

F

F

L

-

T

K

D

R

A

Q

E

D

G

G

K

M

P

P

T

T

T

N

I

I

T

P

H

Q

D

D

K

R

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

I

D

D

L

L

G

A

E

E

N

E

A

I

R

K

R

R

D

E

M

I

V

V

L

L

T

V

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

V

E

E

A

A

S

A

H

H

E
|
E

V

V

A

A

A

F

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

D

D

N

A

A

L

L

D

A

V

T

A

A

D

D

K

N

I

V

V

I

T

T

F

L

K

K

F

Y

V

V

V

A

R

K

T

T

I

L

A

A

Q

L

R

Q

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

I

F

F

G

G

I

V

N

N

G

G

S

S

G

G

M

M

H

H

T

T

N

N

M

T

S

S

L

L

F

F

K

K

D

D

G

G

K

K

N

N

A

A

F

F

Y

Y

D

D

P

P

T

D

T

A

P

P

D

D

Q

Q

L

I

S

S

E

D

T

T

A

L

K

R

Y

Y

Y

F

I

V

G

G

L

V

L

L

K

K

N

H

V

I

R

R

S

A

F

I

A

T

A

A

I

I

T

T

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

Y

Y

E

E

A

A

P

P

C

V

Y

Y

V

I

A

T

W

W

S

S

C

G

R

P

N

N

R

R

S

S

P

S

L

L

I

I

R

R

I

V

P

P

A

A

K

P

R

R

G

G

A

N

S

S

T

T

R

R

V

I

E

E

L

I

R

R

S

S

P

P

D

D

P

P

S

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

V

A

C

I

L

L

K

A

A

A

G

G

L

L

D

D

G

G

I

V

K

K

N

N

K

K

I

I

M

E

P

P

A

E

P

R

V

V

D

E

E

K

N

N

I

I

Y

Y

H

K

M

L

D

T

A

E

R

E

R

R

A

E

E

K

I

L

G

G

I

I

V

G

S

M

L

L

P

P

A

G

N

T

L

L

A

K

E

E

A

A

I

I

D

E

E

C

L

F

E

K

K

E

S

D

E

E

V

L

I

L

K

V

E

S

A

A

L

L

G

G

P

E

H

H

V

V

S

S

E

Q

R

S

Y

I

I

I

E

N

A

V

K

A

R

M

E

A

E

D

W

W

D

D

S

S

F

Y

R

R

I

T

T

Q

V

V

H

H

P

Q

W

W

E

E

V

L

E

D

E

R

Y

Y

L

L

T

Q

K

T

Y

Y

binding magnesium ion: E129 (= E132), E183 (= E187), E190 (= E194)

8oozA Glutamine synthetase (see paper)
60% identity, 99% coverage: 4:442/442 of query aligns to 1:430/430 of 8oozA

query
sites
8oozA

T

S

K

K

E

E

D

D

-

E

V

I

L

F

R

R

M

I

A

V

R

E

E

E

F

K

D

N

V

V

K

R

F

F

I

V

R

R

L

L

Q

Q

F

F

T

V

D

D

I

V

L

Q

G

G

V

I

L

P

K

K

N

N

V

V

A

A

I

I

P

P

V

V

D

G

Q

Q

L

L

Q

E

K

K

A

A

L

L

D

G

G

P

E

G

M

I

M

H

F

F

D

D

G

G

S

S

I

I

D

E

G

G

F

-

V

-

R

-

I

-

E

-

S

S

D

D

M

M

Y

V

L

L

R

R

P

P

D

D

P

P

N

D

T

T

F

F

V

R

I

V

F

L

P

P

W

W

R

-

P

-

Q

-

D

S

G

T

A

A

V

E

A

A

R

R

L

L

I

I

C

C

D

D

V

I

Y

E

N

L

P

P

D

D

G

G

T

K

P

P

F

F

A

M

G

G

D

C

P

P

R

R

N

Q

V

V

L

L

K

K

S

K

V

N

L

M

A

E

E

E

A

A

E

K

M

L

G

G

Y

Y

T

V

M

M

N

N

V

T

G
|
G

P

P

E
|
E

L

M

E

E

F

F

L

L

F

F

L

-

T

K

D

R

A

Q

E

D

G

G

K

M

P

P

T

T

T

N

I

I

T

P

H

Q

D

D

K

R

A

G

G

G

Y

Y

F

F

D

D

M

L

T

A

P

P

I

I

D

D

L

L

G

A

E

E

N

E

A

I

R

K

R

R

D

E

M

I

V

V

L

L

T

V

L

L

E

E

Q

E

M

M

G

G

F

F

E

E

I

V

E
|
E

A

A

S

A

H

H

E

E

V

V

A

A

A

F

G

G

Q

Q

H

H

E

E

I

I

D

D

F
|
F

K
|
K

Y
|
Y

S

D

D

N

A

A

L

L

D

A

V

T

A

A

D

D

K

N

I

V

V

I

T

T

F

L

K

K

F

Y

V

V

V

A

R

K

T

T

I

L

A

A

Q

L

R

Q

H

H

G

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

I

F

F

G

G

I

V

N

N

G

G

S

S

G

G

M

M

H
|
H

T

T

N
|
N

M

T

S
|
S

L

L

F

F

K

K

D

D

G

G

K

K

N

N

A

A

F

F

Y

Y

D

D

P

P

T

D

T

A

P

P

D

D

Q

Q

L

I

S

S

E

D

T

T

A

L

K

R

Y

Y

Y

F

I

V

G

G

L

V

L

L

K

K

N

H

V

I

R

R

S

A

F

I

A

T

A

A

I

I

T

T

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

Y

Y

E

E

A

A

P

P

C

V

Y

Y

V

I

A

T

W

W

S

S

C

G

R

P

N

N

R

R

S

S

P

S

L

L

I

I

R

R

I

V

P

P

A

A

K

P

R

R

G

G

A

N

S
|
S

T

T

R
|
R

V

I

E
|
E

L

I

R

R

S

S

P

P

D

D

P

P

S

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

V

A

C

I

L

L

K

A

A

A

G

G

L

L

D

D

G

G

I

V

K

K

N

N

K

K

I

I

M

E

P

P

A

E

P

R

V

V

D

E

E

K

N

N

I

I

Y

Y

H

K

M

L

D

T

A

E

R

E

R

R

A

E

E

K

I

L

G

G

I

I

V

G

S

M

L

L

P

P

A

G

N

T

L

L

A

K

E

E

A

A

I

I

D

E

E

C

L

F

E

K

K

E

S

D

E

E

V

L

I

L

K

V

E

S

A

A

L

L

G

G

P

E

H

H

V

V

S

S

E

Q

R

S

Y

I

I

I

E

N

A

V

K

A

R

M

E

A

E

D

W

W

D

D

S

S

F

Y

R

R

I

T

T

Q

V

V

H

H

P

Q

W

W

E

E

V

L

E

D

E

R

Y

Y

L

L

T

Q

K

T

Y

Y

binding adenosine-5'-triphosphate: G117 (= G128), E170 (= E182), F185 (= F197), K186 (= K198), Y187 (= Y199), N233 (= N245), S235 (= S247), S315 (= S327), R317 (= R329)
binding magnesium ion: E119 (= E130), H231 (= H243), E319 (= E331)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
55% identity, 99% coverage: 4:439/442 of query aligns to 2:445/447 of 8oooA

query
sites
8oooA

T

T

K

V

E

E

D

Q

V

V

L

L

R

E

M

Y

A

V

R

K

E

S

F

N

D

N

V

V

K

K

F
|
F

I

M

R
|
R

L

F

Q

Q

F

F

T

V

D

D

I

I

L

L

G

G

V

V

L

P

K

K

N

N

V

V

A
|
A

I

F

P

P

V

I

-

K

-

A

-

G

-

E

-

K

-

G

-

I

D

E

Q

E

L

L

Q

R

K

D

A

V

L

L

D

E

G

N

E

G

M

L

M

Y

F

F

D

D

G

G

S

S

I

I

D

E

G

G

F

F

V

V

R

G

I

I

E

N

E

E

S

S

D

D

M

M

Y

M

L

L

R

K

P

P

D

D

P

L

N

S

T

T

F

F

V

S

I

V

F

L

P

P

W

W

R
|
R

P

P

Q

S

D

E

G

K

A

S

V
|
V

A

A

R

R

L

V

I

I

C

C

D

D

V

V

Y

Y

N

T

P

T

D

K

G

G

T

K

P

P

F

F

A

E

G

G

D

D

P

P

R

R

N

G

V

C

L

L

K

K

S

R

V

V

L

M

A

E

E

E

-

F

A

K

E

E

M

F

G

N

Y

G

T

E

M

Y

N

F

V

V

G

G

P

P

E
|
E

L

P

E

E

F

F

L

L

F

L

L

K

T

K

D

D

A

P

E

H

G

N

K

P

P

H

T

K

T

Y

I

I

T

P

H

A

D

D

K

D

A

G

G

G

Y

Y

F

F

D

D

M

L

T

E

P

P

I

M

D

D

L

E

G

A

E
x
P

N

D

A

I

R
|
R

D

D

M

I

V

V

L

F

T

A

L

L

E

E

Q

N

M

L

G

G

F

F

E

H

I

V

E
|
E

A

A

S
|
S

H

H

E

E

V

V

A

A

A

P

G

G

Q

Q

H

H

E

E

I

V

D

D

F
|
F

K
|
K

Y
x
F

S

D

D

D

A

A

L

L

D

K

V

T

A

A

D

D

K

S

I

V

V

I

T

T

F

F

K

K

F

T

V

T

V

I

R

K

T

T

I

I

A

A

Q

E

R

Q

H

H

G

G

L

L

H

K

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

F

F

F

G

G

I

M

N

N

G

G

S

S

G

G

M

M

H

H

T

C

N
x
H

M

Q

S
|
S

L

I

F

W

K

L

D

N

G

G

K

E

N

P

A

S

F

F

Y

Y

D

D

P

E

T

N

T

A

P

P

D

Y

Q

Q

L

L

S

S

E

E

T

T

A

C

K

M

Y

N

Y

Y

I

V

G

A

G

G

L

I

L

L

K

K

N

H

V

A

R

K

S

A

F

I

A

V

A

A

I

I

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

Y

Y

E

E

A

A

P

P

C

V

Y

N

V

I

A

A

W

W

S

A

C

N

R

S

N

N

R

R

S

S

P

A

L

I

I

I

R
|
R

I

V

P

P

A

A

K

A

R

R

G

G

A

K

S

G

T

T

R
|
R

V

I

E

E

L

F

R

R

S

A

P

P

D

D

P

P

S

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

T

V

V

C

M

L

L

K

A

A

A

G

G

L

L

D

D

G

G

I

V

K

K

N

N

K

K

I

L

M

D

P

A

P

P

A

E

P

P

V

V

D

E

E

R

N

N

I

I

Y

F

H

A

M

M

D

S

A

E

A

A

R

E

R

K

A

K

E

E

I

L

G

G

I

I

V

E

S

S

L

V

P

P

A

A

N

N

L

L

A

K

E

A

A

A

I

L

D

D

E

E

L

L

E

E

K

N

S

N

E

D

V

V

I

L

K

K

E

N

A

A

L

L

G

G

P

K

H

H

V

I

S

F

E

E

R

S

Y

F

I

L

E

E

A

I

K

K

R

N

E

A

E

E

W

W

D

D

S

S

F

F

R

R

I

T

T

S

V

V

H

T

P

D

W

W

E

E

V

T

E

T

E

A

Y

Y

L

L

binding 2-oxoglutaric acid: F17 (= F19), R19 (= R21), A33 (= A35), R87 (= R82), V93 (= V88), P170 (≠ E164), R173 (= R167), R174 (= R168), S190 (= S184)
binding adenosine-5'-triphosphate: E136 (= E130), E188 (= E182), F203 (= F197), K204 (= K198), F205 (≠ Y199), H251 (≠ N245), S253 (= S247), R325 (= R319), R335 (= R329)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
55% identity, 99% coverage: 4:439/442 of query aligns to 1:444/446 of 8ooqB

query
sites
8ooqB

T

T

K

V

E

E

D

Q

V

V

L

L

R

E

M

Y

A

V

R

K

E

S

F

N

D

N

V

V

K

K

F
|
F

I

M

R
|
R

L

F

Q

Q

F

F

T

V

D

D

I

I

L

L

G

G

V

V

L

P

K

K

N

N

V

V

A
|
A

I

F

P

P

V

I

-

K

-

A

-

G

-

E

-

K

-

G

-

I

D

E

Q

E

L

L

Q

R

K

D

A

V

L

L

D

E

G

N

E

G

M

L

M

Y

F

F

D

D

G

G

S

S

I

I

D

E

G

G

F

F

V

V

R

G

I

I

E

N

E

E

S

S

D

D

M

M

Y

M

L

L

R

K

P

P

D

D

P

L

N

S

T

T

F

F

V

S

I

V

F

L

P

P

W

W

R
|
R

P

P

Q

S

D

E

G

K

A

S

V
|
V

A

A

R

R

L

V

I

I

C

C

D

D

V

V

Y

Y

N

T

P

T

D

K

G

G

T

K

P

P

F

F

A

E

G

G

D

D

P

P

R

R

N

G

V

C

L

L

K

K

S

R

V

V

L

M

A

E

E

E

-

F

A

K

E

E

M

F

G

N

Y

G

T

E

M

Y

N

F

V

V

G

G

P

P

E

E

L

P

E
|
E

F

F

L

L

F

L

L

K

T

K

D

D

A

P

E

H

G

N

K

P

P

H

T

K

T

Y

I

I

T

P

H

A

D

D

K

D

A

G

G

G

Y

Y

F

F

D

D

M

L

T

E

P

P

I

M

D

D

L

E

G

A

E
x
P

N

D

A

I

R
|
R

D

D

M

I

V

V

L

F

T

A

L

L

E

E

Q

N

M

L

G

G

F

F

E

H

I

V

E

E

A

A

S
|
S

H

H

E
|
E

V

V

A

A

A

P

G

G

Q

Q

H

H

E
|
E

I

V

D

D

F

F

K

K

Y

F

S

D

D

D

A

A

L

L

D

K

V

T

A

A

D

D

K

S

I

V

V

I

T

T

F

F

K

K

F

T

V

T

V

I

R

K

T

T

I

I

A

A

Q

E

R

Q

H

H

G

G

L

L

H

K

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

F

F

F

G

G

I

M

N

N

G

G

S

S

G

G

M

M

H

H

T

C

N

H

M

Q

S

S

L

I

F

W

K

L

D

N

G

G

K

E

N

P

A

S

F

F

Y

Y

D

D

P

E

T

N

T

A

P

P

D

Y

Q

Q

L

L

S

S

E

E

T

T

A

C

K

M

Y

N

Y

Y

I

V

G

A

G

G

L

I

L

L

K

K

N

H

V

A

R

K

S

A

F

I

A

V

A

A

I

I

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

Y

Y

E

E

A

A

P

P

C

V

Y

N

V

I

A

A

W

W

S

A

C

N

R

S

N

N

R

R

S

S

P

A

L

I

I

I

R

R

I

V

P

P

A

A

K

A

R

R

G

G

A

K

S

G

T

T

R

R

V

I

E

E

L

F

R

R

S

A

P

P

D

D

P

P

S

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

T

V

V

C

M

L

L

K

A

A

A

G

G

L

L

D

D

G

G

I

V

K

K

N

N

K

K

I

L

M

D

P

A

P

P

A

E

P

P

V

V

D

E

E

R

N

N

I

I

Y

F

H

A

M

M

D

S

A

E

A

A

R

E

R

K

A

K

E

E

I

L

G

G

I

I

V

E

S

S

L

V

P

P

A

A

N

N

L

L

A

K

E

A

A

A

I

L

D

D

E

E

L

L

E

E

K

N

S

N

E

D

V

V

I

L

K

K

E

N

A

A

L

L

G

G

P

K

H

H

V

I

S

F

E

E

R

S

Y

F

I

L

E

E

A

I

K

K

R

N

E

A

E

E

W

W

D

D

S

S

F

F

R

R

I

T

T

S

V

V

H

T

P

D

W

W

E

E

V

T

E

T

E

A

Y

Y

L

L

binding 2-oxoglutaric acid: F16 (= F19), R18 (= R21), A32 (= A35), R86 (= R82), V92 (= V88), P169 (≠ E164), R172 (= R167), R173 (= R168), S189 (= S184)
binding magnesium ion: E137 (= E132), E192 (= E187), E199 (= E194)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
45% identity, 100% coverage: 1:440/442 of query aligns to 1:445/446 of A0R083

query
sites
A0R083

M

M

G

D

Y

R

T

Q

K

K

E

E

D

F

V

V

L

L

R

R

M

T

A

L

R

E

E

E

F

R

D

D

V

I

K

R

F

F

I

V

R

R

L

L

Q

W

F

F

T

T

D

D

I

V

L

L

G

G

V

Y

L

L

K

K

N

S

V

V

A

A

I

I

P

A

V

P

D

A

Q

E

L

L

Q

E

K

G

A

A

L

F

D

E

G

E

E

G

M

I

M

G

F

F

D

D

G

G

S

S

I

I

D

E

G

G

F

F

V

A

R

R

I

V

E

F

E

E

S

S

D

D

M

T

Y

V

L

A

R

R

P

P

D

D

P

P

N

S

T

T

F

F

V

Q

I

V

F

L

P

P

W

W

R

K

P

T

Q

S

D

D

G

G

A

N

-

H

-

Y

V

S

A

A

R

R

L

M

I

F

C

C

D

D

V

I

Y

T

N

M

P

P

D

D

G

G

T

S

P

P

F

S

A

W

G

A

D

D

P

S

R

R

N

H

V

V

L

L

K

R

S

R

V

Q

L

L

A

A

E

K

A

A

A

S

E

D

M

L

G

G

Y

F

T

T

M

C

N

Y

V

V

G

H

P

P

E

E

L

I

E

E

F

F

L

L

F

L

L

K

T

P

D

G

A

P

E

N

G

D

K

G

P

T

T

P

I

E

T

P

H

A

D

D

K

N

A

G

G

G

Y

Y

F

F

D

D

M

Q

T

A

P

V

I

H

D

D

L

A

G

A

E

P

N

N

A

F

R

R

D

H

M

A

V

I

L

E

T

A

L

L

E

E

Q

Q

M

M

G

G

F

I

E

S

I

V

E

E

A

F

S

S

H

H

E

E

V

G

A

A

A

P

G

G

Q

Q

H

Q

E

E

I

I

D

D

F

L

K

R

Y

Y

S

A

D

D

A

A

L

L

D

S

V

M

A

A

D

D

K

N

I

V

V

M

T

T

F

F

K

R

F

Y

V

L

V

V

R

K

T

E

I

V

A

A

Q

L

R

A

H

D

G

G

L

V

H

R

A

A

T

S

F

F

M

M

P

P

K

K

P

P

I

F

F

A

G

E

I

H

N

P

G

G

S

S

G

A

M

M

H

H

T

T

N

H

M

M

S

S

L

L

F

F

K

E

D

G

G

D

K

T

N

N

A

A

F

F

Y

H

D

S

P

P

T

D

P

P

D

L

Q

Q

L

L

S

S

E

D

T

V

A

A

K

K

Y

S

Y

F

I

I

G

A

G

G

L

I

L

L

K

E

N

H

V

A

R

N

S

E

F

I

A

S

A

A

I

V

T

T

N

N

P

Q

T

W

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

H

G

G

Y

G

E

E

A

A

P

P

C

T

Y

A

V

A

A

S

W

W

S

G

C

A

R

A

N

N

R

R

S

S

P

A

L

L

I

V

R

R

I

V

P

P

A

M

-

Y

-

T

-

P

K

H

R

K

G

V

A

S

S

S

T

R

R

R

V

V

E

E

L

V

R

R

S

S

P

P

D

D

P

S

S

A

C

C

N

N

P

P

Y

Y

L

L

A

T

L

F

A

A

V

V

C

L

L

L

K

A

A

A

G

G

L

L

D

R

G

G

I

V

K

E

N
x
K

K

G

I

Y

M

V

P

L

P

G

A

P

P

Q

V

A

D

E

E

D

N

N

I

V

Y

W

H

S

M

L

D

T

A

Q

A

E

R

E

R

R

A

R

E

A

I

M

G

G

I

Y

V

R

S

E

L

L

P

P

A

T

N

S

L

L

A

G

E

N

A

A

I

L

D

E

E

S

L

M

E

E

K

N

S

S

E

E

V

L

I

V

K

A

E

E

A

A

L

L

G

G

P

E

H

H

V

V

S

F

E

D

R

Y

Y

F

I

L

E

R

A

N

K

K

R

R

E

S

E

E

W

W

D

E

S

N

F

Y

R

R

I

S

T

H

V

V

H

T

P

P

W

Y

E

E

V

L

E

K

E

N

Y

Y

L

L

T

S

K363 (≠ N358) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
44% identity, 100% coverage: 1:440/442 of query aligns to 1:445/446 of P9WN37

query
sites
P9WN37

M

M

G

D

Y

R

T

Q

K

K

E

E

D

F

V

V

L

L

R

R

M

T

A

L

R

E

E

E

F

R

D

D

V

I

K

R

F

F

I

V

R

R

L

L

Q

W

F

F

T

T

D

D

I

V

L

L

G

G

V

F

L

L

K

K

N

S

V

V

A

A

I

I

P

A

V

P

D

A

Q

E

L

L

Q

E

K

G

A

A

L

F

D

E

G

E

E

G

M

I

M

G

F

F

D

D

G

G

S

S

I

I

D

E

G

G

F

F

V

A

R

R

I

V

E

S

E

E

S

S

D

D

M

T

Y

V

L

A

R

H

P

P

D

D

P

P

N

S

T

T

F

F

V

Q

I

V

F

L

P

P

W

W

R

A

P

T

Q

S

D

S

G

G

A

H

-

H

V

S

A

A

R

R

L

M

I

F

C

C

D

D

V

I

Y

T

N

M

P

P

D

D

G

G

T

S

P

P

F

S

A

W

G

A

D

D

P

P

R

R

N

H

V

V

L

L

K

R

S

R

V

Q

L

L

A

T

E

K

A

A

A

G

E

E

M

L

G

G

Y

F

T

S

M

C

N

Y

V

V

G

H

P

P

E

E

L

I

E

E

F

F

L

L

F

L

L

K

T

P

D

G

A

P

E

E

G

D

K

G

P

S

T

V

T

P

I

V

T

P

H

V

D

D

K

N

A

A

G

G

Y

Y

F

F

D

D

M

Q

T

A

P

V

I

H

D

D

L

S

G

A

E

L

N

N

A

F

R

R

D

H

M

A

V

I

L

D

T

A

L

L

E

E

Q

F

M

M

G

G

F

I

E

S

I

V

E

E

A

F

S

S

H

H

E

E

V

G

A

A

A

P

G

G

Q

Q

H

Q

E

E

I

I

D

D

F

L

K

R

Y

F

S

A

D

D

A

A

L

L

D

S

V

M

A

A

D

D

K

N

I

V

V

M

T

T

F

F

K

R

F

Y

V

V

V

I

R

K

T

E

I

V

A

A

Q

L

R

E

H

E

G

G

L

A

H

R

A

A

T

S

F

F

M

M

P

P

K

K

P

P

I

F

F

G

G

Q

I

H

N

P

G

G

S

S

G

A

M

M

H

H

T

T

N

H

M

M

S

S

L

L

F

F

K

E

D

G

G

D

K

V

N

N

A

A

F

F

Y

H

D

S

P

A

T

D

P

P

D

L

Q

Q

L

L

S

S

E

E

T

V

A

G

K

K

Y

S

Y

F

I

I

G

A

G

G

L

I

L

L

K

E

N

H

V

A

R

C

S

E

F

I

A

S

A

A

I

V

T

T

N

N

P

Q

T

W

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

Q

G

G

Y

G

E

E

A

A

P

P

C

T

Y

A

V

A

A

S

W

W

S

G

C

A

R

A

N

N

R

R

S

S

P

A

L

L

I

V

R

R

I

V

P

P

A

M

-

Y

-

T

-

P

K

H

R

K

G

T

A

S

S

S

T

R

R

R

V

V

E

E

L

V

R

R

S

S

P

P

D

D

P

S

S

A

C

C

N

N

P

P

Y

Y

L

L

A

T

L

F

A

A

V

V

C

L

L

L

K

A

A

A

G

G

L

L

D

R

G

G

I

V

K

E

N
x
K

K

G

I

Y

M

V

P

L

P

G

A

P

P

Q

V

A

D

E

E

D

N

N

I

V

Y

W

H

D

M

L

D

T

A

P

A

E

R

E

R

R

A

R

E

A

I

M

G

G

I

Y

V

R

S

E

L

L

P

P

A

S

N

S

L

L

A

D

E

S

A

A

I

L

D

R

E

A

L

M

E

E

K

A

S

S

E

E

V

L

I

V

K

A

E

E

A

A

L

L

G

G

P

E

H

H

V

V

S

F

E

D

R

F

Y

F

I

L

E

R

A

N

K

K

R

R

E

T

E

E

W

W

D

A

S

N

F

Y

R

R

I

S

T

H

V

V

H

T

P

P

W

Y

E

E

V

L

E

R

E

T

Y

Y

L

L

T

S

K363 (≠ N358) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

1fpyA Crystal structure of glutamine synthetase from salmonella typhimurium with inhibitor phosphinothricin (see paper)
43% identity, 99% coverage: 4:440/442 of query aligns to 1:467/468 of 1fpyA

query
sites
1fpyA

T

S

K

A

E

E

D

H

V

V

L

L

R

T

M

M

A

L

R

N

E

E

F

H

D

E

V

V

K

K

F

F

I

V

R

D

L

L

Q

R

F

F

T

T

D

D

I

T

L

K

G

G

V

K

L

E

K

Q

N

H

V

V

A

T

I

I

P

P

V

A

D

H

Q

Q

L

V

Q

N

K

A

A

E

L

F

D

F

G

E

E

E

-

G

M

K

M

M

F

F

D

D

G

G

S

S

I

I

D

G

G

G

F

W

V

K

R

G

I

I

E

N

E

E

S

S

D

D

M

M

Y

V

L

L

R

M

P

P

D

D

P

A

N

S

T

T

F

A

V

V

I

I

F

D

P

P

W

F

R

F

P

A

Q

D

D

S

G

T

A

L

V

I

A

I

R

R

L

-

I

-

C

C

D

D

V

I

Y

L

N

E

P

P

D

-

G

G

T

T

-

L

-

Q

P

G

F

Y

A

D

G

R

D

D

P

P

R

R

N

S

V

I

L

A

K

K

S

R

V

A

-

E

-

D

L

Y

A

L

E

R

A

A

A

T

E

G

M

I

G

A

Y

D

T

T

M

V

N

L

V

F

G
|
G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

-

D

-

I

-

R

-

F

-

G

-

A

-

S

-

I

-

S

-

G

-

S

-

H

-

V

-

A

L

I

T

D

D

D

A

I

E

E

G

G

K

A

P

W

T

N

T

S

I

S

T

T

H

K

-

Y

-

E

-

G

-

N

-

K

-

G

-

H

-

R

-

P

-

G

D

V

K

K

A

G

G

G

Y

Y

F

F

D

P

M

V

T

P

P

P

I

V

D

D

L

S

G

A

E

Q

N

D

A

I

R

R

R

S

D

E

M

M

V

C

L

L

T

V

L

M

E

E

Q

Q

M

M

G

G

F

L

E

V

I

V

E
|
E

A

A

S

H

H

H

E
|
E

V

V

A

A

-

T

A

A

G

G

Q

Q

H

N

E
|
E

I

V

D

A

F
x
T

K

R

Y
x
F

S

N

D

T

A

M

L

T

D

K

V

K

A

A

D

D

K

E

I

I

V

Q

T

I

F

Y

K

K

F

Y

V

V

R

H

T

N

I

V

A

A

Q

H

R

R

H

F

G

G

L

K

H

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

M

F

F

G

G

I

D

N

N

G
|
G

S

S

G

G

M

M

H
|
H

T

C

N
x
H

M

M

S
|
S

L

L

F

A

K

K

D

N

G

G

K

T

N

N

A

L

F

F

Y

S

D

G

P

D

T

K

T

Y

P

A

D

G

Q

-

L

L

S

S

E

E

T

Q

A

A

K

L

Y

Y

I

I

G

G

L

V

L

I

K

K

N

H

V

A

R

K

S

A

F

I

A

N

A

A

I

L

T

A

N

N

P

P

T

T

V

T

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

C

V

Y

M

V

L

A

A

W

Y

S

S

C

A

R

R

N

N

R

R

S

S

P

A

L

S

I

I

R

R

I

I

P

P

A

V

K

V

R

A

G

S

-

P

A

K

S

A

T

R

R
|
R

V

I

E
|
E

L

V

R
|
R

S

F

P

P

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

C

L

F

A

A

V

A

C

L

L

L

K

M

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

N

K

K

I

I

M

H

P

P

P

G

A

E

P

P

V

M

D

D

E

K

N

N

I

L

Y

Y

H

D

M

L

D

P

A

P

A

E

R

E

R

A

A

K

E

E

I

I

G

P

I

Q

V

V

S

A

L

-

P

-

A

G

N

S

L

L

A

E

E

E

A

A

I

L

D

N

E

A

L

L

E

D

K

L

S

D

E

R

V

E

I

F

K

L

E

K

A

A

L

G

G

G

P

V

H

F

V

T

S

D

E

E

-

A

-

I

-

D

R

A

Y

Y

I

I

E

A

A

L

K

R

R

R

E

E

W

D

D

D

S

R

F

V

R

R

I

M

T

T

V

P

H

H

P

P

W

V

E

E

V

F

E

E

E

L

Y

Y

L

Y

T

S

binding adenosine-5'-diphosphate: G127 (= G128), E129 (= E130), E207 (= E182), T223 (≠ F197), F225 (≠ Y199), H271 (≠ N245), S273 (= S247), R355 (= R329), E357 (= E331)
binding manganese (ii) ion: E129 (= E130), E131 (= E132), E212 (= E187), E220 (= E194), H269 (= H243), E357 (= E331)
binding phosphinothricin: E131 (= E132), E212 (= E187), G265 (= G239), H269 (= H243), R321 (= R296), E327 (= E302), R359 (= R333)

1f1hA Crystal structure of glutamine synthetase from salmonella typhimurium with thallium ions (see paper)
43% identity, 99% coverage: 4:440/442 of query aligns to 1:467/468 of 1f1hA

query
sites
1f1hA

T

S

K

A

E

E

D

H

V

V

L

L

R

T

M

M

A

L

R

N

E

E

F

H

D

E

V

V

K

K

F

F

I

V

R

D

L

L

Q

R

F

F

T

T

D

D

I

T

L

K

G

G

V

K

L

E

K

Q

N

H

V

V

A

T

I

I

P

P

V

A

D

H

Q

Q

L

V

Q

N

K

A

A

E

L

F

D

F

G

E

E

E

-

G

M

K

M

M

F

F

D

D

G

G

S

S

I

I

D

G

G

G

F

W

V

K

R

G

I

I

E

N

E

E

S

S

D

D

M

M

Y

V

L

L

R

M

P

P

D

D

P

A

N

S

T

T

F

A

V

V

I

I

F

D

P

P

W

F

R

F

P

A

Q

D

D

S

G

T

A

L

V

I

A

I

R

R

L

-

I

-

C

C

D

D

V

I

Y

L

N

E

P

P

D

-

G

G

T

T

-

L

-

Q

P

G

F

Y

A

D

G

R

D

D

P

P

R

R

N

S

V

I

L

A

K

K

S

R

V

A

-

E

-

D

L

Y

A

L

E

R

A

A

A

T

E

G

M

I

G

A

Y

D

T

T

M

V

N

L

V

F

G

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

F

F

-

D

-

I

-

R

-

F

-

G

-

A

-

S

-

I

-

S

-

G

-

S

-

H

-

V

-

A

L

I

T

D

D

D

A

I

E

E

G

G

K

A

P

W

T

N

T

S

I

S

T

T

H

K

-

Y

-

E

-

G

-

N

-

K

-

G

-

H

-

R

-

P

-

G

D

V

K

K

A

G

G

G

Y

Y

F

F

D

P

M

V

T

P

P

P

I

V

D

D

L

S

G

A

E

Q

N

D

A

I

R

R

R

S

D

E

M

M

V

C

L

L

T

V

L

M

E

E

Q

Q

M

M

G

G

F

L

E

V

I

V

E
|
E

A

A

S

H

H
|
H

H

H

E
|
E

V

V

A

A

-

T

A

A

G

G

Q

Q

H

N

E
|
E

I

V

D

A

F
x
T

K

R

Y
x
F

S

N

D

T

A

M

L

T

D

K

V

K

A

A

D

D

K

E

I

I

V

Q

T

I

F

Y

K

K

F

Y

V

V

R

H

T

N

I

V

A

A

Q

H

R

R

H

F

G

G

L

K

H

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

M

F

F

G

G

I

D

N

N

G

G

S

S

G

G

M

M

H
|
H

T

C

N
x
H

M

M

S
|
S

L

L

F

A

K

K

D

N

G

G

K

T

N

N

A

L

F

F

Y

S

D

G

P

D

T

K

T

Y

P

A

D

G

Q

-

L

L

S

S

E

E

T

Q

A

A

K

L

Y

Y

I

I

G

G

L

V

L

I

K

K

N

H

V

A

R

K

S

A

F

I

A

N

A

A

I

L

T

A

N

N

P

P

T

T

V

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

Y

Y

E

E

A

A

P

P

C

V

Y

M

V

L

A

A

W

Y

S

S

C

A

R

R

N

N

R

R

S

S

P

A

L

S

I

I

R

R

I

I

P

P

A

V

K

V

R

A

G

S

-

P

A

K

S

A

T

R

R
|
R

V

I

E
|
E

L

V

R

R

S

F

P

P

D

D

P

P

S

A

C

A

N

N

P

P

Y

Y

L

L

A

C

L

F

A

A

V

A

C

L

L

L

K

M

A

A

G

G

L

L

D

D

G

G

I

I

K

K

N

N

K

K

I

I

M

H

P

P

P

G

A

E

P

P

V

M

D

D

E

K

N

N

I

L

Y

Y

H

D

M

L

D

P

A

P

A

E

R

E

R

A

A

K

E

E

I

I

G

P

I

Q

V

V

S

A

L

-

P

-

A

G

N

S

L

L

A

E

E

E

A

A

I

L

D

N

E

A

L

L

E

D

K

L

S

D

E

R

V

E

I

F

K

L

E

K

A

A

L

G

G

G

P

V

H

F

V

T

S

D

E

E

-

A

-

I

-

D

R

A

Y

Y

I

I

E

A

A

L

K

R

R

R

E

E

W

D

D

D

S

R

F

V

R

R

I

M

T

T

V

P

H

H

P

P

W

V

E

E

V

F

E

E

E

L

Y

Y

L

Y

T

S

binding adenosine-5'-diphosphate: E129 (= E130), E207 (= E182), H210 (= H185), E220 (= E194), T223 (≠ F197), F225 (≠ Y199), H271 (≠ N245), S273 (= S247), R355 (= R329)
binding manganese (ii) ion: E129 (= E130), E131 (= E132), E212 (= E187), E220 (= E194), H269 (= H243), E357 (= E331)

Query Sequence

>WP_012283382.1 NCBI__GCF_000019165.1:WP_012283382.1
MGYTKEDVLRMAREFDVKFIRLQFTDILGVLKNVAIPVDQLQKALDGEMMFDGSSIDGFV
RIEESDMYLRPDPNTFVIFPWRPQDGAVARLICDVYNPDGTPFAGDPRNVLKSVLAEAAE
MGYTMNVGPELEFFLFLTDAEGKPTTITHDKAGYFDMTPIDLGENARRDMVLTLEQMGFE
IEASHHEVAAGQHEIDFKYSDALDVADKIVTFKFVVRTIAQRHGLHATFMPKPIFGINGS
GMHTNMSLFKDGKNAFYDPTTPDQLSETAKYYIGGLLKNVRSFAAITNPTVNSYKRLVPG
YEAPCYVAWSCRNRSPLIRIPAKRGASTRVELRSPDPSCNPYLALAVCLKAGLDGIKNKI
MPPAPVDENIYHMDAARRAEIGIVSLPANLAEAIDELEKSEVIKEALGPHVSERYIEAKR
EEWDSFRITVHPWEVEEYLTKY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory