SitesBLAST
Comparing WP_012331720.1 NCBI__GCF_000019365.1:WP_012331720.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1x7dA Crystal structure analysis of ornithine cyclodeaminase complexed with NAD and ornithine to 1.6 angstroms (see paper)
57% identity, 97% coverage: 9:347/348 of query aligns to 3:340/340 of 1x7dA
- active site: E55 (= E61), D227 (= D232)
- binding nicotinamide-adenine-dinucleotide: T83 (= T89), R111 (= R117), T112 (= T118), G137 (= G143), A138 (= A144), Q139 (= Q145), D160 (= D166), T161 (≠ I167), V200 (≠ A205), T201 (= T206), A202 (= A207), I209 (= I214), V224 (= V229), G225 (= G230), D227 (= D232), K231 (= K236), S292 (= S297), V293 (= V298), G294 (= G299)
- binding L-ornithine: R44 (= R50), V53 (= V59), E55 (= E61), M57 (= M63), K68 (= K74), V70 (= V76), N71 (= N77), G72 (= G78), R111 (= R117), D227 (= D232), V293 (= V298)
Q88H32 Ornithine cyclodeaminase; OCD; EC 4.3.1.12 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
58% identity, 95% coverage: 9:337/348 of query aligns to 4:334/350 of Q88H32
- R45 (= R50) binding L-ornithine
- K69 (= K74) binding L-ornithine
- T84 (= T89) binding NAD(+)
- R112 (= R117) binding L-ornithine; binding NAD(+)
- AQ 139:140 (= AQ 144:145) binding NAD(+)
- D161 (= D166) binding NAD(+)
- T202 (= T206) binding NAD(+)
- VGGD 225:228 (= VGGD 229:232) binding NAD(+)
- D228 (= D232) binding L-ornithine
- K232 (= K236) binding NAD(+)
- S293 (= S297) binding NAD(+)
- V294 (= V298) binding L-ornithine
- K331 (≠ R334) binding NAD(+)
1u7hA Structure and a proposed mechanism for ornithine cyclodeaminase from pseudomonas putida (see paper)
57% identity, 97% coverage: 9:347/348 of query aligns to 3:340/341 of 1u7hA
- active site: E55 (= E61), D227 (= D232)
- binding nicotinamide-adenine-dinucleotide: T83 (= T89), R111 (= R117), T112 (= T118), G137 (= G143), A138 (= A144), Q139 (= Q145), D160 (= D166), T161 (≠ I167), V200 (≠ A205), T201 (= T206), A202 (= A207), I209 (= I214), V224 (= V229), G225 (= G230), D227 (= D232), K231 (= K236), S292 (= S297), V293 (= V298), G294 (= G299)
O28608 Alanine dehydrogenase; AlaDH; EC 1.4.1.1 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
31% identity, 76% coverage: 49:313/348 of query aligns to 40:306/322 of O28608
1omoA Alanine dehydrogenase dimer w/bound NAD (archaeal) (see paper)
31% identity, 76% coverage: 49:313/348 of query aligns to 40:306/320 of 1omoA
- active site: R52 (≠ E61), D219 (= D232)
- binding nicotinamide-adenine-dinucleotide: T109 (= T118), G134 (= G143), T135 (≠ A144), Q136 (= Q145), Y156 (= Y165), D157 (= D166), V158 (≠ I167), R159 (≠ D168), T195 (≠ D208), P196 (≠ K209), G217 (= G230), D219 (= D232), K223 (= K236), S290 (= S297), T291 (≠ V298), G292 (= G299)
5yu4A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
35% identity, 82% coverage: 56:339/348 of query aligns to 58:343/344 of 5yu4A
- binding 2,4-diaminobutyric acid: E63 (= E61), K77 (= K74), R121 (= R117), T302 (≠ V298), G303 (= G299)
- binding nicotinamide-adenine-dinucleotide: Y81 (≠ G78), T93 (= T89), I94 (≠ V90), R121 (= R117), T122 (= T118), G147 (= G143), A148 (= A144), Q149 (= Q145), D170 (= D166), T171 (≠ I167), H175 (≠ A171), A208 (= A205), T209 (= T206), S210 (≠ A207), V211 (≠ D208), V218 (≠ I214), V233 (= V229), A235 (≠ G231), S301 (= S297), T302 (≠ V298), G303 (= G299)
5yu3A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
35% identity, 82% coverage: 56:339/348 of query aligns to 58:343/344 of 5yu3A
- binding nicotinamide-adenine-dinucleotide: Y81 (≠ G78), T93 (= T89), I94 (≠ V90), T122 (= T118), G147 (= G143), A148 (= A144), Q149 (= Q145), D170 (= D166), T171 (≠ I167), A208 (= A205), T209 (= T206), S210 (≠ A207), V211 (≠ D208), V233 (= V229), A235 (≠ G231), S301 (= S297), T302 (≠ V298), G303 (= G299)
- binding proline: M65 (= M63), K77 (= K74), R121 (= R117)
5gziA Cyclodeaminase_pa
38% identity, 75% coverage: 56:316/348 of query aligns to 62:324/354 of 5gziA
- binding nicotinamide-adenine-dinucleotide: Y85 (≠ G78), T97 (= T89), R125 (= R117), T126 (= T118), G151 (= G143), A152 (= A144), Q153 (= Q145), D174 (= D166), T175 (≠ I167), H179 (≠ A171), A212 (= A205), T213 (= T206), S214 (≠ A207), V215 (≠ D208), V237 (= V229), G238 (= G230), A239 (≠ G231), S305 (= S297), T306 (≠ V298), G307 (= G299)
- binding (2S)-piperidine-2-carboxylic acid: K81 (= K74), R125 (= R117), A239 (≠ G231), T306 (≠ V298), G307 (= G299)
Sites not aligning to the query:
5gzlA Cyclodeaminase_pa
38% identity, 75% coverage: 56:316/348 of query aligns to 62:324/357 of 5gzlA
- binding lysine: I65 (≠ V59), E67 (= E61), D240 (= D232), R267 (≠ I259), E268 (= E260)
- binding nicotinamide-adenine-dinucleotide: Y85 (≠ G78), T97 (= T89), I98 (≠ V90), T126 (= T118), G151 (= G143), A152 (= A144), Q153 (= Q145), D174 (= D166), T175 (≠ I167), H179 (≠ A171), A212 (= A205), T213 (= T206), S214 (≠ A207), V222 (≠ I214), V237 (= V229), G238 (= G230), A239 (≠ G231), D240 (= D232), K244 (= K236), S305 (= S297), T306 (≠ V298), G307 (= G299)
Sites not aligning to the query:
6t3eB Structure of thermococcus litoralis delta(1)-pyrroline-2-carboxylate reductase in complex with nadh and l-proline (see paper)
30% identity, 78% coverage: 57:326/348 of query aligns to 48:325/325 of 6t3eB
- binding 1,4-dihydronicotinamide adenine dinucleotide: S82 (≠ T89), T111 (= T118), G136 (= G143), V137 (≠ A144), Q138 (= Q145), D159 (= D166), I160 (= I167), A199 (= A205), T200 (= T206), T201 (≠ A207), A202 (≠ D208), V206 (≠ I214), V221 (= V229), G222 (= G230), W223 (≠ G231), S296 (= S297), V297 (= V298), G298 (= G299)
- binding proline: M54 (= M63), K67 (= K74), R110 (= R117)
Sites not aligning to the query:
4bvaA Crystal structure of the NADPH-t3 form of mouse mu-crystallin. (see paper)
30% identity, 64% coverage: 95:316/348 of query aligns to 87:301/303 of 4bvaA
- active site: S219 (≠ D232)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T106 (= T114), R109 (= R117), T110 (= T118), G135 (= G143), V136 (≠ A144), Q137 (= Q145), N158 (≠ D166), R159 (≠ I167), T160 (≠ D168), N163 (≠ A171), V194 (≠ A205), T195 (= T206), M196 (≠ A207), A197 (≠ D208), V216 (= V229), S282 (= S297), L283 (≠ V298), G284 (= G299)
- binding 3,5,3'triiodothyronine: S219 (≠ D232), R220 (≠ C233), W223 (≠ K236), E247 (= E260)
Sites not aligning to the query:
4bv9A Crystal structure of the NADPH form of mouse mu-crystallin. (see paper)
30% identity, 64% coverage: 95:316/348 of query aligns to 88:302/303 of 4bv9A
- active site: S220 (≠ D232)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T111 (= T118), G134 (= G141), G136 (= G143), V137 (≠ A144), Q138 (= Q145), N159 (≠ D166), R160 (≠ I167), T161 (≠ D168), V195 (≠ A205), T196 (= T206), M197 (≠ A207), A198 (≠ D208), V217 (= V229), G218 (= G230), S283 (= S297), L284 (≠ V298), G285 (= G299)
- binding pyruvic acid: R110 (= R117)
Sites not aligning to the query:
A1B8Z0 Iminosuccinate reductase; EC 1.4.1.- from Paracoccus denitrificans (strain Pd 1222) (see paper)
33% identity, 72% coverage: 68:318/348 of query aligns to 65:311/320 of A1B8Z0
- R110 (= R117) binding NAD(+)
- HQ 137:138 (≠ AQ 144:145) binding NAD(+)
- N159 (≠ D166) binding NAD(+)
- S199 (≠ D208) binding NAD(+)
- MGTD 219:222 (≠ VGGD 229:232) binding NAD(+)
- K226 (= K236) binding NAD(+)
- G291 (≠ S297) binding NAD(+)
6rqaB Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
33% identity, 72% coverage: 68:318/348 of query aligns to 67:313/322 of 6rqaB
- binding Tb-Xo4: N76 (= N77)
- binding nicotinamide-adenine-dinucleotide: T113 (= T118), G138 (= G143), Q140 (= Q145), P162 (≠ I167), H163 (≠ D168), I199 (≠ T206), T200 (≠ A207), S201 (≠ D208), S202 (≠ K209), M221 (≠ V229), G222 (= G230), D224 (= D232), K228 (= K236), G293 (≠ S297), T294 (≠ V298), G295 (= G299)
Sites not aligning to the query:
6rqaA Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
33% identity, 72% coverage: 68:318/348 of query aligns to 67:313/322 of 6rqaA
- binding nicotinamide-adenine-dinucleotide: H85 (≠ G86), T113 (= T118), G138 (= G143), H139 (≠ A144), Q140 (= Q145), N161 (≠ D166), P162 (≠ I167), H163 (≠ D168), M166 (≠ A171), I199 (≠ T206), T200 (≠ A207), S201 (≠ D208), S202 (≠ K209), M221 (≠ V229), G222 (= G230), D224 (= D232), K228 (= K236), G293 (≠ S297)
2i99A Crystal structure of human mu_crystallin at 2.6 angstrom (see paper)
28% identity, 64% coverage: 95:316/348 of query aligns to 96:310/312 of 2i99A
- active site: S228 (≠ D232)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R118 (= R117), T119 (= T118), G142 (= G141), A143 (≠ N142), G144 (= G143), V145 (≠ A144), Q146 (= Q145), N167 (≠ D166), R168 (≠ I167), T169 (≠ D168), V203 (≠ A205), T204 (= T206), L205 (≠ A207), A206 (≠ D208), V225 (= V229), G226 (= G230), S291 (= S297), L292 (≠ V298), G293 (= G299)
Sites not aligning to the query:
Q14894 Ketimine reductase mu-crystallin; NADP-regulated thyroid-hormone-binding protein; EC 1.5.1.25 from Homo sapiens (Human) (see paper)
28% identity, 64% coverage: 95:316/348 of query aligns to 97:311/314 of Q14894
4mp6A Staphyloferrin b precursor biosynthetic enzyme sbnb bound to citrate and NAD+ (see paper)
26% identity, 78% coverage: 54:326/348 of query aligns to 54:333/334 of 4mp6A
- active site: I60 (= I60), M236 (≠ T237)
- binding nicotinamide-adenine-dinucleotide: R92 (≠ A91), R120 (= R117), T121 (= T118), G146 (= G143), L147 (vs. gap), I148 (vs. gap), D170 (= D166), Q171 (≠ I167), C211 (≠ A205), T212 (= T206), V213 (≠ A207), I233 (≠ V229), G306 (= G299)
4m54A The structure of the staphyloferrin b precursor biosynthetic enzyme sbnb bound to n-(1-amino-1-carboxyl-2-ethyl)-glutamic acid and nadh (see paper)
25% identity, 80% coverage: 49:326/348 of query aligns to 44:309/310 of 4m54A
- active site: I55 (= I60), M231 (≠ T237)
- binding N-[(2S)-2-amino-2-carboxyethyl]-L-glutamic acid: Y45 (≠ R50), R53 (≠ G58), M57 (= M63), K71 (= K74), I73 (≠ V76), R87 (≠ A91), R115 (= R117)
- binding 1,4-dihydronicotinamide adenine dinucleotide: R87 (≠ A91), T116 (= T118), G141 (= G143), L142 (vs. gap), I143 (vs. gap), D165 (= D166), Q166 (≠ I167), T207 (= T206), T209 (≠ D208), I228 (≠ V229)
4mpdA Staphyloferrin b precursor biosynthetic enzyme sbnb bound a- ketoglutarate and NAD+ (see paper)
25% identity, 73% coverage: 72:326/348 of query aligns to 62:317/318 of 4mpdA
- active site: M224 (≠ T237)
- binding 2-oxoglutaric acid: K64 (= K74), I66 (≠ V76), R80 (≠ A91), M289 (≠ V298)
- binding nicotinamide-adenine-dinucleotide (acidic form): R80 (≠ A91), R108 (= R117), T109 (= T118), G134 (= G143), I136 (vs. gap), D158 (= D166), Q159 (≠ I167), F160 (≠ D168), T200 (= T206), V201 (≠ A207), I221 (≠ V229)
Sites not aligning to the query:
Query Sequence
>WP_012331720.1 NCBI__GCF_000019365.1:WP_012331720.1
MRALNTVPFVSVENMMRLVLAIGVERVLTDLAACIEADFRRWDSFDKTPRVAAHSVDGVI
ELMPTSDGTSYGFKYVNGHPKNLRSGRQTVAAFGVLADVSHGYPVLLTEMTILTALRTAA
TSAVAARVLAPPGARTMALIGNGAQAEFQAMAFKALLGIDRLRLYDIDPAATAKCLRNLA
GLGFDLQPCTSVAAAVEGAEIVTTATADKQNATILTDNLVGPGIHINGVGGDCPGKTELH
PDVLRRAEITVEYAPQTRIEGEIQQLPPDHPVTELWQVLAGRAPGRRDARAVTLFDSVGF
AIEDFSALRYVREALAIHPFFTELDLVADPDEPRDLFGMLLRARARAA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory