SitesBLAST
Comparing WP_012333956.1 NCBI__GCF_000019365.1:WP_012333956.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 15 hits to proteins with known functional sites (download)
2j5tD Glutamate 5-kinase from escherichia coli complexed with glutamate (see paper)
41% identity, 97% coverage: 9:368/373 of query aligns to 3:362/365 of 2j5tD
P0A7B5 Glutamate 5-kinase; Gamma-glutamyl kinase; GK; EC 2.7.2.11 from Escherichia coli (strain K12) (see paper)
41% identity, 97% coverage: 9:368/373 of query aligns to 5:364/367 of P0A7B5
- S50 (= S55) binding substrate
- D137 (= D142) binding substrate
- N149 (= N154) binding substrate
2j5vA Glutamate 5-kinase from escherichia coli complexed with glutamyl-5- phosphate and pyroglutamic acid (see paper)
36% identity, 97% coverage: 9:368/373 of query aligns to 3:320/323 of 2j5vA
- binding magnesium ion: K8 (= K14), G10 (= G16), L166 (≠ F173)
- binding pyroglutamic acid: T11 (≠ S17), S48 (= S55), G49 (= G56), A50 (≠ S57), I51 (= I58)
- binding gamma-glutamyl phosphate: K8 (= K14), G10 (= G16), S48 (= S55), D135 (= D142), D146 (= D153), N147 (= N154)
2j5vB Glutamate 5-kinase from escherichia coli complexed with glutamyl-5- phosphate and pyroglutamic acid (see paper)
37% identity, 97% coverage: 9:368/373 of query aligns to 3:322/325 of 2j5vB
- binding pyroglutamic acid: T11 (≠ S17), G49 (= G56), A50 (≠ S57), I51 (= I58), A52 (= A59), D135 (= D142)
- binding gamma-glutamyl phosphate: K8 (= K14), S48 (= S55), D135 (= D142), G145 (= G152), D146 (= D153), N147 (= N154)
8j0gB Gk monomer complexes with glutamate and atp
33% identity, 66% coverage: 9:255/373 of query aligns to 8:262/274 of 8j0gB
- binding adenosine-5'-triphosphate: K13 (= K14), G15 (= G16), T16 (≠ S17), A17 (= A18), V185 (≠ I176), G191 (≠ A182), P193 (= P184), F215 (≠ G208), R223 (= R216), G225 (= G218), M226 (= M219), K229 (= K222)
- binding gamma-l-glutamic acid: A55 (≠ S57), N147 (= N139), D150 (= D142), N163 (= N154), R223 (= R216)
- binding magnesium ion: R223 (= R216), G224 (= G217), G225 (= G218)
- binding : Y65 (≠ F67), L68 (vs. gap), V69 (vs. gap), F73 (vs. gap)
7wx3B Gk domain of drosophila p5cs filament with glutamate, atp, and NADPH (see paper)
30% identity, 68% coverage: 9:261/373 of query aligns to 13:257/258 of 7wx3B
- binding gamma-glutamyl phosphate: S59 (= S55), A61 (≠ S57), N149 (= N139), T150 (≠ E140), D152 (= D142), M158 (≠ E148), N165 (= N154)
- binding : E72 (≠ P68), M75 (≠ G70), L77 (= L72), S78 (≠ R73), M79 (≠ L74), R80 (≠ E75)
2akoA Crystal structure of glutamate 5-kinase from campylobacter jejuni
33% identity, 62% coverage: 9:241/373 of query aligns to 1:219/241 of 2akoA
- binding adenosine-5'-diphosphate: H10 (≠ A18), S161 (= S174), D162 (= D175), I163 (= I176), F166 (≠ L179), Y167 (= Y180), N170 (≠ P183), P171 (= P184), T194 (≠ R216), G196 (= G218), K200 (= K222)
8j0eB Gk monomer complexes with catalytic intermediate
33% identity, 66% coverage: 9:255/373 of query aligns to 11:257/269 of 8j0eB
- binding magnesium ion: D163 (= D155), G219 (= G217)
- binding (2~{R})-5-[[[[(2~{R},3~{S},4~{S},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-2-azanyl-5-oxidanylidene-pentanoic acid: K16 (= K14), G18 (= G16), T19 (≠ S17), A20 (= A18), S56 (= S55), G57 (= G56), V59 (≠ I58), D153 (= D142), N162 (= N154), V184 (≠ I176), P192 (= P184), K217 (≠ P210), R218 (= R216), G219 (= G217), G220 (= G218), M221 (= M219)
- binding : Y68 (≠ F67), L71 (vs. gap), V72 (vs. gap), V72 (vs. gap), S74 (vs. gap), F76 (vs. gap), F76 (vs. gap), Q80 (vs. gap)
7f5xA Gk domain of drosophila p5cs filament with glutamate (see paper)
36% identity, 48% coverage: 9:187/373 of query aligns to 13:185/236 of 7f5xA
8j0fA Gk tetramer with adjacent hooks at reaction state (see paper)
33% identity, 66% coverage: 9:255/373 of query aligns to 12:256/270 of 8j0fA
- binding adenosine-5'-diphosphate: A21 (= A18), D183 (= D175), V184 (≠ I176), Y188 (= Y180), G190 (≠ A182), F214 (≠ A207), G219 (= G218), M220 (= M219)
- binding magnesium ion: R217 (= R216), G218 (= G217), G219 (= G218)
- binding gamma-glutamyl phosphate: K17 (= K14), T20 (≠ S17), S57 (= S55), D154 (= D142), N162 (= N154), R217 (= R216)
- binding : L72 (vs. gap), V73 (vs. gap), N74 (≠ P68), S75 (≠ P69), S76 (≠ G70), F77 (≠ A71), F77 (≠ A71), A78 (≠ L72), L80 (= L74)
3wwmA Crystal structure of lysz from thermus thermophilus with adp (see paper)
33% identity, 31% coverage: 140:253/373 of query aligns to 163:263/269 of 3wwmA
Sites not aligning to the query:
O50147 [LysW]-aminoadipate kinase; EC 2.7.2.17 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
33% identity, 31% coverage: 140:253/373 of query aligns to 163:263/269 of O50147
- R227 (≠ G218) mutation to E: Still binds LysW, but shows reduced activity.
- R230 (≠ T221) mutation to E: Still binds LysW, but shows reduced activity.
- K231 (= K222) mutation to E: Still binds LysW, but shows reduced activity.
Sites not aligning to the query:
- 57 H→A: Shows reduced activity.
- 111 R→E: Shows reduced activity.
- 112 R→E: Shows reduced activity.
- 113 K→E: Loss of activity.
- 117 K→E: Does not bind LysW.
- 123 K→E: Does not bind LysW.
- 125 K→E: Does not bind LysW.
- 128 R→E: Does not bind LysW.
Q8U122 Uridylate kinase; UK; Uridine monophosphate kinase; UMP kinase; UMPK; EC 2.7.4.22 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see paper)
34% identity, 23% coverage: 154:238/373 of query aligns to 120:201/225 of Q8U122
- T120 (≠ N154) binding Mg(2+)
- D121 (= D155) binding Mg(2+); binding Mg(2+)
- G179 (= G218) binding UMP
- S182 (≠ T221) binding Mg(2+)
Sites not aligning to the query:
- 6 binding Mg(2+)
- 44 binding UMP
- 66 binding UMP
- 114:120 binding UMP
2bmuB Ump kinase from pyrococcus furiosus complexed with its substrate ump and its substrate analog amppnp (see paper)
34% identity, 23% coverage: 154:238/373 of query aligns to 121:202/226 of 2bmuB
- binding phosphoaminophosphonic acid-adenylate ester: T141 (≠ S174), N142 (≠ D175), V146 (≠ L179), Y147 (= Y180), A149 (= A182), D150 (≠ P183), P151 (= P184), S182 (≠ R220), S183 (≠ T221), V184 (≠ K222)
- binding magnesium ion: D122 (= D155), D122 (= D155), S183 (≠ T221), V184 (≠ K222)
- binding uridine-5'-monophosphate: T121 (≠ N154), A179 (≠ G217)
Sites not aligning to the query:
- binding phosphoaminophosphonic acid-adenylate ester: 9, 10, 44, 45, 46
- binding magnesium ion: 7
- binding uridine-5'-monophosphate: 44, 45, 67, 70, 71, 114, 115, 116, 119, 120
2ji5A Structure of ump kinase from pyrococcus furiosus complexed with utp
43% identity, 13% coverage: 154:202/373 of query aligns to 122:167/219 of 2ji5A
Sites not aligning to the query:
Query Sequence
>WP_012333956.1 NCBI__GCF_000019365.1:WP_012333956.1
MTPALDQFRRVVLKVGSALLVDRARGRLRHAWLAALAEDIADLHGRGVDVLVVSSGSIAL
GRTVLGFPPGALRLEESQAAAAVGQIALARHWSEALAHHGIVAGQILVTPHDTEERRRYL
NARATTLKLLDMRAVPVVNENDTVATSEIRYGDNDRLAARVATMIGADLLVLFSDIDGLY
TAPPASDPSARHLPVIPRITPEIEAMAGGPASELSRGGMRTKIEAGKIAAGGGTHMLIAD
GRGKNPLRAVAAGARASWFLSASTPTAARKTWIAGSLEPRGSLVIDAGAERALQGGASLL
PVGVARIEGAFARGDAVVIRSLGGAVLGRGLVAYDSDDAARIIGRSSREIEAVLGYPGRA
EMIHRDDMALVGE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory