SitesBLAST
Comparing WP_012335976.1 NCBI__GCF_000019365.1:WP_012335976.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5jc8D Crystal structure of a putative short-chain dehydrogenase/reductase from burkholderia xenovorans
47% identity, 96% coverage: 4:255/262 of query aligns to 3:258/262 of 5jc8D
7krmC Putative fabg bound to nadh from acinetobacter baumannii
40% identity, 95% coverage: 5:253/262 of query aligns to 3:240/244 of 7krmC
- active site: G18 (= G23), S140 (= S148), Y155 (= Y162)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), S15 (= S17), G18 (= G23), I19 (≠ N24), D38 (= D43), L39 (= L45), A60 (= A68), N61 (≠ D69), V62 (= V70), N88 (= N96), V111 (= V119), S140 (= S148), Y155 (= Y162), K159 (= K166), I188 (≠ L195), T190 (= T197)
8y46B SDR family oxidoreductase (see paper)
37% identity, 96% coverage: 4:255/262 of query aligns to 1:247/248 of 8y46B
8y4jA SDR family oxidoreductase (see paper)
37% identity, 96% coverage: 4:255/262 of query aligns to 2:248/249 of 8y4jA
8y11A Crystal structure of l-2-keto-3-deoxyfuconate 4-dehydrogenase bound to NAD(h) and sulfate ion (see paper)
37% identity, 96% coverage: 4:255/262 of query aligns to 2:248/249 of 8y11A
- binding nicotinamide-adenine-dinucleotide: A12 (≠ G14), Q15 (≠ S17), G16 (= G23), I17 (≠ N24), D36 (= D43), I37 (≠ R44), D57 (= D69), V58 (= V70), C80 (≠ N96), I130 (= I146), Y146 (= Y162), K150 (= K166), P176 (= P192), G177 (= G193), T178 (≠ L194), I179 (≠ L195), S181 (≠ T197), P182 (≠ S198), S183 (≠ L199), R187 (≠ C203)
8y46A SDR family oxidoreductase (see paper)
37% identity, 96% coverage: 4:255/262 of query aligns to 4:250/251 of 8y46A
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
36% identity, 97% coverage: 1:255/262 of query aligns to 1:259/261 of 6zzsD
- active site: G18 (= G23), S143 (= S148), Y156 (= Y162)
- binding nicotinamide-adenine-dinucleotide: G14 (= G14), S17 (= S17), I19 (≠ N24), D38 (= D43), M39 (≠ R44), D64 (= D69), V65 (= V70), N91 (= N96), A92 (≠ V97), G93 (= G98), M141 (≠ I146), A142 (≠ S147), S143 (= S148), Y156 (= Y162), K160 (= K166), P186 (= P192), G187 (= G193), V189 (≠ L195), T191 (= T197), L193 (= L199)
- binding 3-oxidanylidenepentanoic acid: Q95 (≠ A100), S143 (= S148), N145 (≠ A150), K153 (≠ A157), Y156 (= Y162), Q197 (≠ C203)
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
36% identity, 96% coverage: 5:255/262 of query aligns to 4:258/260 of 6zzqA
- active site: G17 (= G23), S142 (= S148), Y155 (= Y162)
- binding acetoacetic acid: Q94 (≠ A100), S142 (= S148), K152 (≠ A157), Y155 (= Y162), Q196 (≠ C203)
- binding nicotinamide-adenine-dinucleotide: G13 (= G14), S16 (= S17), G17 (= G23), I18 (≠ N24), D37 (= D43), M38 (≠ R44), D63 (= D69), V64 (= V70), N90 (= N96), A91 (≠ V97), G92 (= G98), M140 (≠ I146), A141 (≠ S147), S142 (= S148), Y155 (= Y162), K159 (= K166), Y187 (≠ L194), V188 (≠ L195), T190 (= T197)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
38% identity, 95% coverage: 5:253/262 of query aligns to 3:244/247 of 4jroC
- active site: G16 (= G19), S142 (= S148), Q152 (≠ A158), Y155 (= Y162), K159 (= K166)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), S14 (= S17), R15 (≠ A18), G16 (= G19), I17 (≠ T20), N35 (vs. gap), Y36 (≠ V42), N37 (≠ D43), G38 (≠ R44), S39 (≠ L45), N63 (≠ D69), V64 (= V70), N90 (= N96), A91 (≠ V97), I93 (≠ L99), I113 (≠ V119), S142 (= S148), Y155 (= Y162), K159 (= K166), P185 (= P192), I188 (≠ L195), T190 (= T197)
A7IQH5 2-(S)-hydroxypropyl-CoM dehydrogenase 3; S-HPCDH 3; 2-[(S)-2-hydroxypropylthio]ethanesulfonate dehydrogenase 3; Aliphatic epoxide carboxylation component IV; Epoxide carboxylase component IV; SHPCDH3; EC 1.1.1.269 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 2 papers)
38% identity, 85% coverage: 34:255/262 of query aligns to 29:253/255 of A7IQH5
- D38 (= D43) binding NAD(+)
- DV 64:65 (= DV 69:70) binding NAD(+)
- N91 (= N96) binding NAD(+)
- S143 (= S148) binding (S)-2-hydroxypropyl-coenzyme M; mutation to A: Retains very weak activity.
- Y156 (= Y162) binding (S)-2-hydroxypropyl-coenzyme M; mutation to A: Retains some activity but with more than 2200-fold decrease in catalytic efficiency.; mutation to F: Loss of activity.
- K160 (= K166) binding NAD(+); mutation to A: Loss of activity.
- T188 (vs. gap) binding (S)-2-hydroxypropyl-coenzyme M
- VTSTG 189:193 (≠ LLDTS 194:198) binding NAD(+)
- R211 (= R213) mutation to A: Severely impaired in the oxidation of S-HPC or reduction of 2-KPC but largely unaffected in the oxidation and reduction of aliphatic alcohols and ketones.
- K214 (≠ L216) mutation to A: Severely impaired in the oxidation of S-HPC or reduction of 2-KPC but largely unaffected in the oxidation and reduction of aliphatic alcohols and ketones.
- Y215 (≠ V217) binding (S)-2-hydroxypropyl-coenzyme M
Sites not aligning to the query:
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
35% identity, 98% coverage: 1:257/262 of query aligns to 4:253/258 of 4wecA
- active site: G21 (= G19), S143 (= S148), Q154 (≠ A159), Y157 (= Y162), K161 (= K166)
- binding nicotinamide-adenine-dinucleotide: G17 (= G14), A19 (≠ S17), S20 (≠ A18), G21 (= G19), I22 (≠ T20), D41 (= D43), I42 (≠ A49), V61 (≠ A68), D62 (= D69), V63 (= V70), N89 (= N96), T141 (≠ I146), Y157 (= Y162), K161 (= K166), P187 (= P192), P189 (≠ L194), V190 (≠ L195)
4gh5A Crystal structure of s-2-hydroxypropyl coenzyme m dehydrogenase (s- hpcdh) (see paper)
39% identity, 85% coverage: 34:255/262 of query aligns to 27:246/248 of 4gh5A
- active site: N113 (= N120), S141 (= S148), Y154 (= Y162), K158 (= K166)
- binding nicotinamide-adenine-dinucleotide: D36 (= D43), L37 (≠ R44), A61 (= A68), D62 (= D69), V63 (= V70), N89 (= N96), A90 (≠ V97), V112 (= V119), F139 (≠ I146), S141 (= S148), Y154 (= Y162), K158 (= K166), P184 (= P192), V187 (≠ L195), T190 (≠ L199), G191 (≠ R200), M192 (≠ D201)
Sites not aligning to the query:
4ituA Crystal structure of s-2-hydroxypropyl coenzyme m dehydrogenase (s- hpcdh) bound to s-hpc and nadh (see paper)
38% identity, 85% coverage: 34:255/262 of query aligns to 27:251/253 of 4ituA
- active site: N113 (= N120), S141 (= S148), Y154 (= Y162), K158 (= K166)
- binding 2-{[(2S)-2-hydroxypropyl]sulfanyl}ethanesulfonic acid: S141 (= S148), Y154 (= Y162), T186 (vs. gap), R209 (= R213), Y213 (≠ V217)
- binding 1,4-dihydronicotinamide adenine dinucleotide: D36 (= D43), L37 (≠ R44), D62 (= D69), V63 (= V70), N89 (= N96), V112 (= V119), F139 (≠ I146), S141 (= S148), Y154 (= Y162), K158 (= K166), P184 (= P192), T186 (vs. gap), V187 (≠ L194), T190 (= T197), M192 (≠ L199)
Sites not aligning to the query:
1g6kA Crystal structure of glucose dehydrogenase mutant e96a complexed with NAD+
33% identity, 97% coverage: 1:255/262 of query aligns to 1:251/261 of 1g6kA
- active site: G18 (= G23), S145 (= S148), Y158 (= Y162), K162 (= K166)
- binding nicotinamide-adenine-dinucleotide: T17 (≠ S17), G18 (= G23), L19 (≠ N24), R39 (= R44), D65 (= D69), V66 (= V70), N92 (= N96), A93 (≠ V97), G94 (= G98), M143 (≠ I146), S145 (= S148), Y158 (= Y162), P188 (= P192), G189 (= G193), I191 (≠ L195), T193 (= T197)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
35% identity, 95% coverage: 5:253/262 of query aligns to 6:239/243 of 4i08A
- active site: G19 (= G23), N113 (= N120), S141 (= S148), Q151 (≠ A158), Y154 (= Y162), K158 (= K166)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G14), S17 (≠ G16), R18 (≠ S17), I20 (≠ N24), T40 (≠ R44), N62 (≠ D69), V63 (= V70), N89 (= N96), A90 (≠ V97), G140 (≠ S147), S141 (= S148), Y154 (= Y162), K158 (= K166), P184 (= P192), G185 (= G193), T189 (= T197)
Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
37% identity, 91% coverage: 19:256/262 of query aligns to 20:265/267 of Q9LBG2
- E103 (≠ A100) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.
Sites not aligning to the query:
1iy8A Crystal structure of levodione reductase (see paper)
37% identity, 92% coverage: 19:258/262 of query aligns to 11:258/258 of 1iy8A
- active site: G15 (= G23), S143 (= S148), Q153 (≠ A158), Y156 (= Y162), K160 (= K166)
- binding nicotinamide-adenine-dinucleotide: G11 (= G19), S14 (≠ W22), G15 (= G23), L16 (≠ N24), D35 (= D43), V36 (≠ R44), A62 (= A68), D63 (= D69), V64 (= V70), N90 (= N96), G92 (= G98), I93 (≠ L99), T141 (≠ I146), S143 (= S148), Y156 (= Y162), K160 (= K166), P186 (= P192), G187 (= G193), T191 (= T197), P192 (≠ S198), M193 (≠ L199)
4urfB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
38% identity, 95% coverage: 5:253/262 of query aligns to 3:244/248 of 4urfB
- active site: G16 (= G23), S142 (= S148), I152 (≠ A157), Y155 (= Y162), K159 (= K166)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: L210 (= L219), R211 (≠ G220), R212 (= R221)
- binding bicarbonate ion: I92 (≠ L99), G94 (≠ E101), R109 (= R115), R179 (= R186), S228 (= S237)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G14 (= G16), N15 (≠ S17), G16 (= G23), I17 (≠ N24), D36 (= D43), I37 (≠ R44), D62 (= D69), T63 (≠ V70), N89 (= N96), A90 (≠ V97), G91 (= G98), I140 (= I146), Y155 (= Y162), K159 (= K166), P185 (= P192), A186 (≠ G193), I188 (≠ L195), T190 (= T197)
4urfA Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
38% identity, 95% coverage: 5:253/262 of query aligns to 3:244/248 of 4urfA
- active site: G16 (= G23), S142 (= S148), I152 (≠ A157), Y155 (= Y162), K159 (= K166)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: I92 (≠ L99), S93 (≠ A100), G94 (≠ E101), E95 (≠ P102), T97 (≠ G104), E101 (≠ D107), T103 (≠ D109), Q106 (≠ A112), R109 (= R115), S175 (≠ R182), G177 (= G184)
- binding magnesium ion: S237 (≠ V246), Y238 (≠ C247)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G14 (= G16), N15 (≠ S17), G16 (= G23), I17 (≠ N24), D36 (= D43), I37 (≠ R44), W41 (≠ A48), D62 (= D69), T63 (≠ V70), N89 (= N96), A90 (≠ V97), G91 (= G98), I140 (= I146), Y155 (= Y162), K159 (= K166), P185 (= P192), I188 (≠ L195), T190 (= T197)
4ureB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
38% identity, 95% coverage: 5:253/262 of query aligns to 3:244/248 of 4ureB
- active site: G16 (= G23), S142 (= S148), I152 (≠ A157), Y155 (= Y162), K159 (= K166)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: N15 (≠ S17), G16 (= G23), I17 (≠ N24), N89 (= N96), G91 (= G98), Y155 (= Y162), P185 (= P192), A186 (≠ G193)
Query Sequence
>WP_012335976.1 NCBI__GCF_000019365.1:WP_012335976.1
MPDRLIGKTAIVFGAGSAGTGWGNGKAAAVAFAREGARVVCVDRLAEAAEETAHLIAEEG
NDAEALAADVTDSEAVVRAVAAAERRFGGIDILHNNVGLAEPGGPEDLDEAAWRRAIEVN
VGGVWRTCRAVLPGMRARRRGAVVNISSVASIRWTGAAAFAYGAAKAAVNQATVAVAMQY
ARDGIRANAILPGLLDTSLRDDCRAAGTRDAGRDRLVPLGRLGEAWDIAHAAVFLASDEA
RFITGVCLPVDGGQSCSMAHLA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory