SitesBLAST

W22 (≠ R28) mutation to A: Decreased heat stability.
E26 (≠ D32) mutation to Q: Increased dissociation of dodecamers into trimers.
M30 (≠ G36) mutation to A: Increased dissociation of dodecamers into trimers.
W34 (≠ K40) mutation to A: Increased dissociation of dodecamers into trimers.
Y228 (≠ I232) mutation to C: Becomes active at low temperatures; when associated with G-278.
A241 (≠ E245) mutation to D: Becomes active at low temperatures; when associated with G-278.
E278 (= E282) mutation to G: Becomes active at low temperatures; when associated with C-228 or D-241.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q81M99 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Bacillus anthracis
46% identity, 95% coverage: 11:314/319 of query aligns to 9:309/316 of Q81M99

query
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STRT 57:60 (= STRT 65:68) binding carbamoyl phosphate
Q84 (= Q92) binding carbamoyl phosphate
R108 (= R116) binding carbamoyl phosphate
HPCQ 135:138 (= HPCQ 143:146) binding carbamoyl phosphate
N166 (= N174) binding L-ornithine
D230 (= D234) binding L-ornithine
SM 234:235 (= SM 238:239) binding L-ornithine
CL 269:270 (= CL 274:275) binding carbamoyl phosphate
R297 (= R302) binding carbamoyl phosphate

4nf2A Crystal structure of anabolic ornithine carbamoyltransferase from bacillus anthracis in complex with carbamoyl phosphate and l- norvaline
46% identity, 95% coverage: 11:314/319 of query aligns to 5:305/307 of 4nf2A

query
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4nf2A

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active site: R55 (= R67), T56 (= T68), R83 (= R95), R104 (= R116), H131 (= H143), Q134 (= Q146), D226 (= D234), C265 (= C274), R293 (= R302)
binding phosphoric acid mono(formamide)ester: S53 (= S65), T54 (= T66), R55 (= R67), T56 (= T68), R104 (= R116), H131 (= H143), Q134 (= Q146), C265 (= C274), L266 (= L275), R293 (= R302)
binding norvaline: L126 (= L138), N162 (= N174), D226 (= D234), S230 (= S238), M231 (= M239)

8qeuA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with ornithine (see paper)
45% identity, 95% coverage: 14:315/319 of query aligns to 2:303/304 of 8qeuA

query
sites
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binding L-ornithine: L125 (= L138), N161 (= N174), D222 (= D234), S226 (= S238), M227 (= M239), L263 (= L275)

8qevA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with carbamoyl phosphate (see paper)
44% identity, 95% coverage: 14:315/319 of query aligns to 2:296/297 of 8qevA

query
sites
8qevA

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H

H

A

A

Q

Q

K

N

G

A

I

I

L

M

A

L

W

H

C

L

F

L

G

G

binding phosphoric acid mono(formamide)ester: S52 (= S65), M53 (≠ T66), R54 (= R67), T55 (= T68), R103 (= R116), C255 (= C274), L256 (= L275), R283 (= R302)

P00481 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Rattus norvegicus (Rat) (see 2 papers)
42% identity, 93% coverage: 5:302/319 of query aligns to 30:330/354 of P00481

query
sites
P00481

P
|
P

V
|
V

-
x
Q

S

S

D

Q

L

V

I

Q

V

L

Q

K

P

G

K

R

H

D

F

L

L

L

D

T

L

L

S

K

R

N

L

F

E

T

A

G

K

E

D

E

L

I

R

Q

A

Y

I

M

L

L

D

W

L

L

A

S

A

A

G

D

L

L

K

K

A

F

K

-

R

-

R

R

R

I

G

K

Q

Q

K

K

A

G

K

E

E

Y

Q

L

P

P

L

L

-

L

E

Q

G

G

K

K

V

S

L

L

A

G

M

M

V

I

F

F

D

E

Q

K

P

R

S

S

T

T

R
|
R

T

T

R

R

V

L

S

S

F

T

D

E

L

T

A

G

M

F

R

A

E

L

L

L

G

G

E

H

T

P

I

S

M

F

L

L

T

T

G

T

Q

Q

E

D

M

I

Q

H

L

L

G

G

R

V

G

N

E

E

T

S

I

L

A

T

D

D

T

T

A

A

R

R

V

V

L

L

S

S

R

S

F

M

V

T

D

D

A

A

I

V

V

L

I

A

R

R

I

V

L

Y

N

K

H

Q

A

S

D

D

L

L

L

D

E

I

L

L

A

A

E

K

Y

E

A

A

E

T

V

I

P

P

V

I

I

V

N

N

G

G

L

L

T

S

K

D

L

L

S

Y

H

H

P

P

C

I

Q

Q

I

I

M

L

A

A

D

D

L

Y

L

L

T

T

F

L

E

Q

E

E

H

H

R

Y

G

G

P

S

I

L

K

K

G

G

R

L

T

T

I

L

A

S

W

W

T

I

G

G

D

D

S

G

N

N

V

I

L

L

A

H

S

S

W

I

I

M

E

M

A

S

A

A

R

A

R

K

L

F

D

G

F

M

T

H

L

L

N

Q

V

A

A

A

C

T

P

P

A

K

E

G

L

Y

S

E

P

P

A

D

P

P

E

N

L

I

V

V

A

K

-

L

-

A

-

E

-

Q

F

Y

A

A

N

K

A

E

Q

N

G

G

K

T

R

R

L

L

N

S

V

M

L

T

R

N

D

D

P

P

F

L

E

E

A

A

V

A

R

R

G

G

A

G

D

N

A

V

V

L

I

I

S

T

D

D

C

T

W

W

V

I

S

S

M

M

G

G

D

Q

E

E

E

D

E

E

E

K

A

K

R

K

R

R

Q

L

A

Q

L

A

L

F

S

Q

P

G

Y

Y

Q

Q

V

V

N

T

A

M

E

K

L

T

M

A

A

K

T

V

A

A

K

S

D

D

A

W

I

T

F

F

M

L

H

H

C
|
C

L

L

P

P

A

-

H

R

R

K

G

P

E

E

V

V

T

D

D

D

E

E

V

V

I

F

D

Y

G

S

P

P

Q

R

S

S

V

L

V

V

F

F

D

P

E

E

A

A

E

E

N

N

R

R

R92 (= R67) mutation to L: Strong decrease in ornithine carbamoyltransferase activity.
C303 (= C274) mutation to S: Increases KM for ornithine 5-fold and decreases kcat 20-fold.

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

1othA Crystal structure of human ornithine transcarbamoylase complexed with n-phosphonacetyl-l-ornithine (see paper)
41% identity, 92% coverage: 11:302/319 of query aligns to 4:297/321 of 1othA

query
sites
1othA

V

L

Q

K

P

G

K

R

H

D

F

L

L

L

D

T

L

L

S

K

R

N

L

F

E

T

A

G

K

E

D

E

L

I

R

K

A

Y

I

M

L

L

D

W

L

L

A

S

A

A

G

D

L

L

K

K

A

F

K

-

R

-

R

R

R

I

G

K

Q

Q

K

K

A

G

K

E

E

Y

Q

L

P

P

L

L

-

L

E

Q

G

G

K

K

V

S

L

L

A

G

M

M

V

I

F

F

D

E

Q

K

P

R

S
|
S

T
|
T

R
|
R

T
|
T

R

R

V

L

S

S

F

T

D

E

L

T

A

G

M

F

R

A

E

L

L

L

G

G

E

H

T

P

I

C

M

F

L

L

T

T

G

T

Q

Q

E

D

M

I

Q

H

L

L

G

G

R
x
V

G

N

E

E

T

S

I

L

A

T

D

D

T

T

A

A

R

R

V

V

L

L

S

S

R

S

F

M

V

A

D

D

A

A

I

V

V

L

I

A

R
|
R

I

V

L

Y

N

K

H

Q

A

S

D

D

L

L

L

D

E

T

L

L

A

A

E

K

Y

E

A

A

E

S

V

I

P

P

V

I

I

I

N

N

G

G

L
|
L

T

S

K

D

L

L

S

Y

H
|
H

P

P

C

I

Q
|
Q

I

I

M

L

A

A

D

D

L

Y

L

L

T

T

F

L

E

Q

E

E

H

H

R

Y

G

S

P

S

I

L

K

K

G

G

R

L

T

T

I

L

A

S

W

W

T

I

G

G

D

D

S

G

N

N

N
|
N

V

I

L

L

A

H

S

S

W

I

I

M

E

M

A

S

A

A

R

A

R

K

L

F

D

G

F

M

T

H

L

L

N

Q

V

A

A

A

C

T

P

P

A

K

E

G

L

Y

S

E

P

P

A

D

P

A

E

S

L

V

V

T

A

K

-

L

-

A

-

E

-

Q

F

Y

A

A

N

K

A

E

Q

N

G

G

K

T

R

K

L

L

N

L

V

L

L

T

R

N

D

D

P

P

F

L

E

E

A

A

V

A

R

H

G

G

A

G

D

N

A

V

V

L

I

I

S

T

D
|
D

C

T

W

W

V

I

S
|
S

M
|
M

G

G

D

R

E

E

E

K

A

K

R

K

R

R

Q

L

A

Q

L

A

L

F

S

Q

P

G

Y

Y

Q

Q

V

V

N

T

A

M

E

K

L

T

M

A

A

K

T

V

A

A

K

S

D

D

A

W

I

T

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

-

H

R

R

K

G

P

E

E

V

V

T

D

D

D

E

E

V

V

I

F

D

Y

G

S

P

P

Q

R

S

S

V

L

V

V

F

F

D

P

E

E

A

A

E

E

N

N

R
|
R

active site: R59 (= R67), T60 (= T68), V87 (≠ R95), R108 (= R116), H135 (= H143), Q138 (= Q146), D230 (= D234), C270 (= C274), R297 (= R302)
binding n-(phosphonoacetyl)-l-ornithine: S57 (= S65), T58 (= T66), R59 (= R67), T60 (= T68), R108 (= R116), L130 (= L138), H135 (= H143), N166 (= N174), D230 (= D234), S234 (= S238), M235 (= M239), C270 (= C274), L271 (= L275), R297 (= R302)

1c9yA Human ornithine transcarbamylase: crystallographic insights into substrate recognition and catalytic mechanism (see paper)
41% identity, 92% coverage: 11:302/319 of query aligns to 4:297/321 of 1c9yA

query
sites
1c9yA

V

L

Q

K

P

G

K

R

H

D

F

L

L

L

D

T

L

L

S

K

R

N

L

F

E

T

A

G

K

E

D

E

L

I

R

K

A

Y

I

M

L

L

D

W

L

L

A

S

A

A

G

D

L

L

K

K

A

F

K

-

R

-

R

R

R

I

G

K

Q

Q

K

K

A

G

K

E

E

Y

Q

L

P

P

L

L

-

L

E

Q

G

G

K

K

V

S

L

L

A

G

M

M

V

I

F

F

D

E

Q

K

P

R

S
|
S

T
|
T

R
|
R

T
|
T

R

R

V

L

S

S

F

T

D

E

L

T

A

G

M

F

R

A

E

L

L

L

G

G

E

H

T

P

I

C

M

F

L

L

T

T

G

T

Q

Q

E

D

M

I

Q

H

L

L

G

G

R
x
V

G

N

E

E

T

S

I

L

A

T

D

D

T

T

A

A

R

R

V

V

L

L

S

S

R

S

F

M

V

A

D

D

A

A

I

V

V

L

I

A

R
|
R

I

V

L

Y

N

K

H

Q

A

S

D

D

L

L

L

D

E

T

L

L

A

A

E

K

Y

E

A

A

E

S

V

I

P

P

V

I

I

I

N

N

G

G

L
|
L

T

S

K

D

L

L

S

Y

H
|
H

P

P

C

I

Q
|
Q

I

I

M

L

A

A

D

D

L

Y

L

L

T

T

F

L

E

Q

E

E

H

H

R

Y

G

S

P

S

I

L

K

K

G

G

R

L

T

T

I

L

A

S

W

W

T

I

G

G

D

D

S

G

N

N

N
|
N

V

I

L

L

A

H

S

S

W

I

I

M

E

M

A

S

A

A

R

A

R

K

L

F

D

G

F

M

T

H

L

L

N

Q

V

A

A

A

C

T

P

P

A

K

E

G

L

Y

S

E

P

P

A

D

P

A

E

S

L

V

V

T

A

K

-

L

-

A

-

E

-

Q

F

Y

A

A

N

K

A

E

Q

N

G

G

K

T

R

K

L

L

N

L

V

L

L

T

R

N

D

D

P

P

F

L

E

E

A

A

V

A

R

H

G

G

A

G

D

N

A

V

V

L

I

I

S

T

D
|
D

C

T

W

W

V

I

S
|
S

M
|
M

G

G

D

R

E

E

E

K

A

K

R

K

R

R

Q

L

A

Q

L

A

L

F

S

Q

P

G

Y

Y

Q

Q

V

V

N

T

A

M

E

K

L

T

M

A

A

K

T

V

A

A

K

S

D

D

A

W

I

T

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

-

H

R

R

K

G

P

E

E

V

V

T

D

D

D

E

E

V

V

I

F

D

Y

G

S

P

P

Q

R

S

S

V

L

V

V

F

F

D

P

E

E

A

A

E

E

N

N

R
|
R

active site: R59 (= R67), T60 (= T68), V87 (≠ R95), R108 (= R116), H135 (= H143), Q138 (= Q146), D230 (= D234), C270 (= C274), R297 (= R302)
binding phosphoric acid mono(formamide)ester: S57 (= S65), T58 (= T66), R59 (= R67), T60 (= T68), R108 (= R116), C270 (= C274), L271 (= L275), R297 (= R302)
binding norvaline: L130 (= L138), N166 (= N174), D230 (= D234), S234 (= S238), M235 (= M239)

P00480 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Homo sapiens (Human) (see 31 papers)
41% identity, 92% coverage: 11:302/319 of query aligns to 37:330/354 of P00480

query
sites
P00480

V

L

Q

K

P
x
G

K
x
R

H

D

F

L

L
|
L

D

T

L

L

S
x
K

R

N

L

F

E

T

A

G

K

E

D

E

L

I

R

K

A
x
Y

I

M

L

L

D

W

L

L

A

S

A

A

G

D

L
|
L

K

K

A

F

K

-

R

-

R

R

R

I

G

K

Q

Q

K

K

A

G

K

E

E

Y

Q

L

P

P

L

L

-

L

E

Q

G

G

K

K

V

S

L

L

A

G

M

M

V

I

F

F

D

E

Q
x
K

P

R

S
|
S

T
|
T

R
|
R

T
|
T

R

R

V

L

S

S

F

T

D

E

L

T

A
x
G

M
x
F

R

A

E

L

L

L

G

G

E

H

T

P

I

C

M

F

L
|
L

T

T

G

T

Q

Q

E

D

M

I

Q

H

L

L

G

G

R

V

G

N

E

E

T

S

I

L

A
x
T

D
|
D

T

T

A

A

R
|
R

V

V

L

L

S

S

R

S

F

M

V

A

D

D

A

A

I

V

V

L

I
x
A

R
|
R

I

V

L

Y

N

K

H

Q

A

S

D

D

L

L

L

D

E

T

L

L

A

A

E

K

Y

E

A

A

E

S

V

I

P

P

V

I

I

I

N

N

G

G

L

L

T

S

K

D

L

L

S

Y

H
|
H

P

P

C

I

Q
|
Q

I
|
I

M

L

A

A

D

D

L
x
Y

L

L

T
|
T

F
x
L

E

Q

E

E

H

H

R
x
Y

G

S

P

S

I

L

K

K

G
|
G

R

L

T

T

I

L

A

S

W

W

T

I

G
|
G

D
|
D

S

G

N

N

V

I

L
|
L

A

H

S

S

W

I

I

M

E

M

A
x
S

A

A

R
x
A

R

K

L

F

D

G

F
x
M

T
x
H

L

L

N

Q

V

A

A

A

C

T

P
|
P

A

K

E

G

L

Y

S

E

P
|
P

A

D

P

A

E

S

L

V

V

T

A

K

-

L

-

A

-

E

-

Q

F

Y

A

A

N

K

A

E

Q

N

G

G

K

T

R

K

L
|
L

N

L

V

L

L

T

R

N

D

D

P

P

F

L

E

E

A

A

V

A

R

H

G

G

A

G

D

N

A

V

V

L

I

I

S
x
T

D

D

C
x
T

W
|
W

V

I

S

S

M

M

G
|
G

D
x
Q

E

E

E
|
E

E

E

E

K

A

K

R

K

R
|
R

Q

L

A

Q

L

A

L

F

S

Q

P

G

Y

Y

Q

Q

V

V

N

T

A

M

E

K

L

T

M

A

A

K

T

V

A

A

K

S

D

D

A

W

I

T

F

F

M

L

H
|
H

C
|
C

L
|
L

P

P

A

-

H

R

R

K

G

P

E
|
E

E

E

V

V

T

D

D

D

E

E

V

V

I

F

D

Y

G

S

P

P

Q

R

S

S

V

L

V

V

F

F

D

P

E

E

A

A

E

E

N

N

R
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G39 (≠ P13) to C: in OTCD; late onset; dbSNP:rs72554306
R40 (≠ K14) to H: in OTCD; late onset; dbSNP:rs72554308
L43 (= L17) to F: in dbSNP:rs72554309
K46 (≠ S20) to R: in dbSNP:rs1800321
Y55 (≠ A29) to D: in OTCD; late onset; dbSNP:rs72554319
L63 (= L37) to P: in OTCD; late onset; dbSNP:rs72554324
K88 (≠ Q63) modified: N6-acetyllysine; alternate; to N: in OTCD; late onset; dbSNP:rs72554339
STRT 90:93 (= STRT 65:68) binding carbamoyl phosphate
G100 (≠ A75) to D: in OTCD; late onset; dbSNP:rs72554349
F101 (≠ M76) to L: in dbSNP:rs1133135
L111 (= L86) to P: in dbSNP:rs1800324
T125 (≠ A100) to M: in OTCD; neonatal; dbSNP:rs72554356
D126 (= D101) to G: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; 0.9% of wild-type activity; dbSNP:rs72554358
R129 (= R104) to H: in OTCD; early onset; decreased ornithine carbamoyltransferase activity; 2.1% of wild-type activity; dbSNP:rs66656800
A140 (≠ I115) to P: in OTCD; late onset; dbSNP:rs72556260
R141 (= R116) binding carbamoyl phosphate; to Q: in OTCD; most common variant; loss of ornithine carbamoyltransferase activity; activity is 100-fold lower; dbSNP:rs68026851
H168 (= H143) binding carbamoyl phosphate
Q171 (= Q146) binding carbamoyl phosphate
I172 (= I147) to M: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; dbSNP:rs72556280
Y176 (≠ L151) to C: in OTCD; late onset; dbSNP:rs72556283
TL 178:179 (≠ TF 153:154) natural variant: Missing (in OTCD; neonatal)
Y183 (≠ R158) to D: in OTCD; late onset; dbSNP:rs72556292
G188 (= G163) to R: in OTCD; neonatal; dbSNP:rs72556294
G195 (= G170) to R: in OTCD; loss of ornithine carbamoyltransferase activity; dbSNP:rs67294955
D196 (= D171) to V: in OTCD; neonatal; decreased ornithine carbamoyltransferase activity; 3.7% activity; dbSNP:rs72556300
L201 (= L176) to P: in OTCD; neonatal; dbSNP:rs72558407
S207 (≠ A182) to R: in OTCD; neonatal; dbSNP:rs72558415
A209 (≠ R184) to V: in OTCD; neonatal; dbSNP:rs72558417
M213 (≠ F188) to K: in OTCD; late onset
H214 (≠ T189) to Y: in OTCD; neonatal; dbSNP:rs72558420
P220 (= P195) to A: in OTCD; late onset; dbSNP:rs72558425
P225 (= P200) to T: in OTCD; late onset; dbSNP:rs72558428
L244 (= L215) to Q: in OTCD; late onset; dbSNP:rs72558436
T262 (≠ S233) to K: in OTCD; mild; dbSNP:rs67333670
T264 (≠ C235) to A: in OTCD; late onset; decreased ornithine carbamoyltransferase activity; 8.9% activity; dbSNP:rs72558444; to I: in OTCD; late onset; dbSNP:rs67156896
W265 (= W236) to L: in OTCD; mild; dbSNP:rs72558446
G269 (= G240) to E: in OTCD; neonatal; dbSNP:rs72558450
Q270 (≠ D241) to R: in dbSNP:rs1800328
E272 (= E243) natural variant: Missing (in OTCD; late onset; dbSNP:rs72558452)
R277 (= R248) to Q: in OTCD; late onset; dbSNP:rs66724222; to W: in OTCD; late onset; dbSNP:rs72558454
H302 (= H273) to L: in OTCD; female; late onset; dbSNP:rs67993095; to Y: in OTCD; neonatal; dbSNP:rs72558463
C303 (= C274) to R: in OTCD; neonatal; dbSNP:rs67468335
CL 303:304 (= CL 274:275) binding carbamoyl phosphate
E309 (= E281) natural variant: Missing (in OTCD; late onset)
R330 (= R302) binding carbamoyl phosphate

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion
15 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-23 and G-26.
23 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-26.
26 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-23.
333 natural variant: T -> A
340 S → P: in OTCD; late onset; dbSNP:rs72558489
343 T → K: in OTCD; late onset; dbSNP:rs72558491

7nouA Crystal structure of mycobacterium tuberculosis argf in complex with (3,5-dichlorophenyl)boronic acid.
43% identity, 94% coverage: 14:314/319 of query aligns to 4:305/308 of 7nouA

query
sites
7nouA

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active site: R102 (= R116), H129 (= H143), Q132 (= Q146), D225 (= D234), C265 (= C274), R293 (= R302)
binding [3,5-bis(chloranyl)phenyl]-oxidanyl-oxidanylidene-boron: I46 (≠ V60), T52 (= T66), R53 (= R67), R53 (= R67), F56 (≠ V70), F56 (≠ V70), L79 (= L93), D82 (≠ G96), E83 (= E97), V91 (= V105), Y95 (≠ F109), L266 (= L275), R293 (= R302)

7nosA Crystal structure of mycobacterium tuberculosis argf in complex with 4-bromo-6-(trifluoromethyl)-1h-benzo[d]imidazole.
43% identity, 94% coverage: 14:314/319 of query aligns to 4:305/308 of 7nosA

query
sites
7nosA

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active site: R102 (= R116), H129 (= H143), Q132 (= Q146), D225 (= D234), C265 (= C274), R293 (= R302)
binding 4-bromanyl-6-(trifluoromethyl)-1~{H}-benzimidazole: I46 (≠ V60), D82 (≠ G96), E83 (= E97), V91 (= V105), L92 (= L106)

7norA Crystal structure of mycobacterium tuberculosis argf in complex with 2-fluoro-4-hydroxybenzonitrile.
43% identity, 94% coverage: 14:314/319 of query aligns to 4:305/308 of 7norA

query
sites
7norA

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active site: R102 (= R116), H129 (= H143), Q132 (= Q146), D225 (= D234), C265 (= C274), R293 (= R302)
binding 2-fluoro-4-hydroxybenzonitrile: T52 (= T66), F56 (≠ V70), T77 (≠ M91), L79 (= L93)

7nnyA Crystal structure of mycobacterium tuberculosis argf in complex with naphthalen-1-ol.
43% identity, 94% coverage: 14:314/319 of query aligns to 4:305/308 of 7nnyA

query
sites
7nnyA

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A

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I

I

V

V

I

W

R
|
R

I

T

L

F

N

G

H

Q

A

E

D

R

L

L

L

D

E

A

L

M

A

A

E

S

Y

V

A

A

E

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

K

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

L

L

L

Q

T

T

F

I

E

A

E

E

H

R

R

K

G

G

P

A

I

L

K

R

G

G

R

L

T

R

I

L

A

S

W

Y

T

F

G

G

D

D

-

G

S

A

N

N

V

M

L

A

A

H

S

S

W

L

I

L

E

L

A

G

R

V

R

T

L

A

D

G

F

I

T

H

L

V

N

T

V

V

A

A

C

A

P

P

A

E

E

G

L

F

S

L

P

P

A

D

P

P

E

S

L

V

V

R

A

A

F

A

A

A

N

E

A

R

Q

R

-

A

-

Q

-

D

-

T

G

G

K

A

R

S

L

V

N

T

V

V

L

T

R

A

D

D

P

A

F

H

E

A

A

A

V

A

R

A

G

G

A

A

D

D

A

V

V

L

I

V

S

T

D
|
D

C

T

W

W

V

T

S

S

M

M

G

G

D

Q

E

E

E

N

E

D

E

G

A

L

R

D

R

R

Q

V

A

K

L

P

L

F

S

R

P

P

Y

F

Q

Q

V

L

N

N

A

S

E

R

L

L

M

L

A

A

T

L

A

A

A

D

K

S

D

D

A

A

I

I

F

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

T

T

D

D

E

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

F

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

G

A

I

L

L

L

A

V

W

W

C

L

F

L

active site: R102 (= R116), H129 (= H143), Q132 (= Q146), D225 (= D234), C265 (= C274), R293 (= R302)
binding 1-naphthol: T52 (= T66), R53 (= R67), F56 (≠ V70), E83 (= E97), V91 (= V105), Y95 (≠ F109)

7nnwA Crystal structure of mycobacterium tuberculosis argf in complex with methyl 4-hydroxy-3-iodobenzoate.
43% identity, 94% coverage: 14:314/319 of query aligns to 4:305/308 of 7nnwA

query
sites
7nnwA

K

R

H

H

F

F

L

L

D

R

L

D

S

D

R

D

L

L

E

S

A

P

K

A

D

E

L

Q

R

A

A

E

I

V

L

L

D

E

L

L

A

A

G

E

L

L

K

K

A

K

K

-

R

-

G

-

Q

D

K

P

A

V

K

S

E

R

Q

R

P

P

L

L

E

Q

G

G

-

P

K

R

V

G

L

V

A

A

M

V

V
x
I

F

F

D

D

Q

K

P

N

S

S

T
|
T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

D

E

L

L

A

G

M

I

R

A

E

Q

L

L

G

G

E

H

T

A

I

V

M

V

L

V

T

D

G

S

Q

G

E

S

M

T

Q

Q

L
|
L

G

G

R

R

G

D

E

E

T

T

I

L

A

Q

D

D

T

T

A

A

R

K

V

V

L
|
L

S

S

R

R

F
x
Y

V

V

D

D

A

A

I

I

V

V

I

W

R
|
R

I

T

L

F

N

G

H

Q

A

E

D

R

L

L

L

D

E

A

L

M

A

A

E

S

Y

V

A

A

E

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

K

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

L

L

L

Q

T

T

F

I

E

A

E

E

H

R

R

K

G

G

P

A

I

L

K

R

G

G

R

L

T

R

I

L

A

S

W

Y

T

F

G

G

D

D

-

G

S

A

N

N

V

M

L

A

A

H

S

S

W

L

I

L

E

L

A

G

R

V

R

T

L

A

D

G

F

I

T

H

L

V

N

T

V

V

A

A

C

A

P

P

A

E

E

G

L

F

S

L

P

P

A

D

P

P

E

S

L

V

V

R

A

A

F

A

A

A

N

E

A

R

Q

R

-

A

-

Q

-

D

-

T

G

G

K

A

R

S

L

V

N

T

V

V

L

T

R

A

D

D

P

A

F

H

E

A

A

A

V

A

R

A

G

G

A

A

D

D

A

V

V

L

I

V

S

T

D
|
D

C

T

W

W

V

T

S

S

M

M

G

G

D

Q

E

E

E

N

E

D

E

G

A

L

R

D

R

R

Q

V

A

K

L

P

L

F

S

R

P

P

Y

F

Q

Q

V

L

N

N

A

S

E

R

L

L

M

L

A

A

T

L

A

A

A

D

K

S

D

D

A

A

I

I

F

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

T

T

D

D

E

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

F

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

G

A

I

L

L

L

A

V

W

W

C

L

F

L

active site: R102 (= R116), H129 (= H143), Q132 (= Q146), D225 (= D234), C265 (= C274), R293 (= R302)
binding methyl 3-iodanyl-4-oxidanyl-benzoate: I46 (≠ V60), T52 (= T66), R53 (= R67), F56 (≠ V70), L79 (= L93), L92 (= L106), Y95 (≠ F109)

7nnvA Crystal structure of mycobacterium tuberculosis argf in complex with carbamoyl phosphate.
43% identity, 94% coverage: 14:314/319 of query aligns to 4:305/308 of 7nnvA

query
sites
7nnvA

K

R

H

H

F

F

L

L

D

R

L

D

S

D

R

D

L

L

E

S

A

P

K

A

D

E

L

Q

R

A

A

E

I

V

L

L

D

E

L

L

A

A

G

E

L

L

K

K

A

K

K

-

R

-

G

-

Q

D

K

P

A

V

K

S

E

R

Q

R

P

P

L

L

E

Q

G

G

-

P

K

R

V

G

L

V

A

A

M

V

V

I

F

F

D

D

Q

K

P

N

S
|
S

T
|
T

R
|
R

T
|
T

R

R

V

F

S

S

F

F

D

E

L

L

A

G

M

I

R

A

E

Q

L

L

G

G

E

H

T

A

I

V

M

V

L

V

T

D

G

S

Q

G

E

S

M

T

Q

Q

L

L

G

G

R

R

G

D

E

E

T

T

I

L

A

Q

D

D

T

T

A

A

R

K

V

V

L

L

S

S

R

R

F

Y

V

V

D

D

A

A

I

I

V

V

I

W

R
|
R

I

T

L

F

N

G

H

Q

A

E

D

R

L

L

L

D

E

A

L

M

A

A

E

S

Y

V

A

A

E

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

K

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

L

L

L

Q

T

T

F

I

E

A

E

E

H

R

R

K

G

G

P

A

I

L

K

R

G

G

R

L

T

R

I

L

A

S

W

Y

T

F

G

G

D

D

-

G

S

A

N

N

V

M

L

A

A

H

S

S

W

L

I

L

E

L

A

G

R

V

R

T

L

A

D

G

F

I

T

H

L

V

N

T

V

V

A

A

C

A

P

P

A

E

E

G

L

F

S

L

P

P

A

D

P

P

E

S

L

V

V

R

A

A

F

A

A

A

N

E

A

R

Q

R

-

A

-

Q

-

D

-

T

G

G

K

A

R

S

L

V

N

T

V

V

L

T

R

A

D

D

P

A

F

H

E

A

A

A

V

A

R

A

G

G

A

A

D

D

A

V

V

L

I

V

S

T

D
|
D

C

T

W

W

V

T

S

S

M

M

G

G

D

Q

E

E

E

N

E

D

E

G

A

L

R

D

R

R

Q

V

A

K

L

P

L

F

S

R

P

P

Y

F

Q

Q

V

L

N

N

A

S

E

R

L

L

M

L

A

A

T

L

A

A

A

D

K

S

D

D

A

A

I

I

F

V

M

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

T

T

D

D

E

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

F

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

G

A

I

L

L

L

A

V

W

W

C

L

F

L

active site: R102 (= R116), H129 (= H143), Q132 (= Q146), D225 (= D234), C265 (= C274), R293 (= R302)
binding phosphoric acid mono(formamide)ester: S51 (= S65), T52 (= T66), R53 (= R67), T54 (= T68), R102 (= R116), H129 (= H143), C265 (= C274), L266 (= L275), R293 (= R302)

P9WIT9 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
43% identity, 94% coverage: 14:314/319 of query aligns to 3:304/307 of P9WIT9

query
sites
P9WIT9

K

R

H

H

F

F

L

L

D

R

L

D

S

D

R

D

L

L

E

S

A

P

K

A

D

E

L

Q

R

A

A

E

I

V

L

L

D

E

L

L

A

A

G

E

L

L

K

K

A

K

K

-

R

-

G

-

Q

D

K

P

A

V

K

S

E

R

Q

R

P

P

L

L

E

Q

G

G

-

P

K

R

V

G

L

V

A

A

M

V

V

I

F

F

D

D

Q

K

P

N

S
|
S

T
|
T

R
|
R

T
|
T

R

R

V

F

S

S

F

F

D

E

L

L

A

G

M

I

R

A

E

Q

L

L

G

G

E

H

T

A

I

V

M

V

L

V

T

D

G

S

Q

G

E

S

M

T

Q
|
Q

L

L

G

G

R

R

G

D

E

E

T

T

I

L

A

Q

D

D

T

T

A

A

R

K

V

V

L

L

S

S

R

R

F

Y

V

V

D

D

A

A

I

I

V

V

I

W

R
|
R

I

T

L

F

N

G

H

Q

A

E

D

R

L

L

L

D

E

A

L

M

A

A

E

S

Y

V

A

A

E

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

K

D

L

E

S

F

H
|
H

P
|
P

C
|
C

Q
|
Q

I

V

M

L

A

A

D

D

L

L

L

Q

T

T

F

I

E

A

E

E

H

R

R

K

G

G

P

A

I

L

K

R

G

G

R

L

T

R

I

L

A

S

W

Y

T

F

G

G

D

D

-

G

S

A

N

N

N
|
N

V

M

L

A

A

H

S

S

W

L

I

L

E

L

A

G

R

V

R

T

L

A

D

G

F

I

T

H

L

V

N

T

V

V

A

A

C

A

P

P

A

E

E

G

L

F

S

L

P

P

A

D

P

P

E

S

L

V

V

R

A

A

F

A

A

A

N

E

A

R

Q

R

-

A

-

Q

-

D

-

T

G

G

K

A

R

S

L

V

N

T

V

V

L

T

R

A

D

D

P

A

F

H

E

A

A

A

V

A

R

A

G

G

A

A

D

D

A

V

V

L

I

V

S

T

D
|
D

C

T

W

W

V

T

S
|
S

M
|
M

G

G

D

Q

E

E

E

N

E

D

E

G

A

L

R

D

R

R

Q

V

A

K

L

P

L

F

S

R

P

P

Y

F

Q

Q

V

L

N

N

A

S

E

R

L

L

M

L

A

A

T

L

A

A

A

D

K

S

D

D

A

A

I

I

F

V

M

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

T

T

D

D

E

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

F

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

G

A

I

L

L

L

A

V

W

W

C

L

F

L

STRT 50:53 (= STRT 65:68) binding carbamoyl phosphate
Q77 (= Q92) binding carbamoyl phosphate
R101 (= R116) binding carbamoyl phosphate
HPCQ 128:131 (= HPCQ 143:146) binding carbamoyl phosphate
N160 (= N174) binding L-ornithine
D224 (= D234) binding L-ornithine
SM 228:229 (= SM 238:239) binding L-ornithine
CL 264:265 (= CL 274:275) binding carbamoyl phosphate
R292 (= R302) binding carbamoyl phosphate

2i6uA Crystal structure of ornithine carbamoyltransferase complexed with carbamoyl phosphate and l-norvaline from mycobacterium tuberculosis (rv1656) at 2.2 a (see paper)
43% identity, 94% coverage: 14:314/319 of query aligns to 3:304/307 of 2i6uA

query
sites
2i6uA

K

R

H

H

F

F

L

L

D

R

L

D

S

D

R

D

L

L

E

S

A

P

K

A

D

E

L

Q

R

A

A

E

I

V

L

L

D

E

L

L

A

A

G

E

L

L

K

K

A

K

K

-

R

-

G

-

Q

D

K

P

A

V

K

S

E

R

Q

R

P

P

L

L

E

Q

G

G

-

P

K

R

V

G

L

V

A

A

M

V

V

I

F

F

D

D

Q

K

P

N

S
|
S

T
|
T

R
|
R

T
|
T

R

R

V

F

S

S

F

F

D

E

L

L

A

G

M

I

R

A

E

Q

L

L

G

G

E

H

T

A

I

V

M

V

L

V

T

D

G

S

Q

G

E

S

M

T

Q

Q

L

L

G

G

R
|
R

G

D

E

E

T

T

I

L

A

Q

D

D

T

T

A

A

R

K

V

V

L

L

S

S

R

R

F

Y

V

V

D

D

A

A

I

I

V

V

I

W

R
|
R

I

T

L

F

N

G

H

Q

A

E

D

R

L

L

L

D

E

A

L

M

A

A

E

S

Y

V

A

A

E

T

V

V

P

P

V

V

I

I

N

N

G

A

L
|
L

T

S

K

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

L

L

L

Q

T

T

F

I

E

A

E

E

H

R

R

K

G

G

P

A

I

L

K

R

G

G

R

L

T

R

I

L

A

S

W

Y

T

F

G

G

D

D

-

G

S

A

N

N

N
|
N

V

M

L

A

A

H

S

S

W

L

I

L

E

L

A

G

R

V

R

T

L

A

D

G

F

I

T

H

L

V

N

T

V

V

A

A

C

A

P

P

A

E

E

G

L

F

S

L

P

P

A

D

P

P

E

S

L

V

V

R

A

A

F

A

A

A

N

E

A

R

Q

R

-

A

-

Q

-

D

-

T

G

G

K

A

R

S

L

V

N

T

V

V

L

T

R

A

D

D

P

A

F

H

E

A

A

A

V

A

R

A

G

G

A

A

D

D

A

V

V

L

I

V

S

T

D
|
D

C

T

W

W

V

T

S
|
S

M
|
M

G

G

D

Q

E

E

E

N

E

D

E

G

A

L

R

D

R

R

Q

V

A

K

L

P

L

F

S

R

P

P

Y

F

Q

Q

V

L

N

N

A

S

E

R

L

L

M

L

A

A

T

L

A

A

A

D

K

S

D

D

A

A

I

I

F

V

M

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

T

T

D

D

E

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

F

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

G

A

I

L

L

L

A

V

W

W

C

L

F

L

active site: R52 (= R67), T53 (= T68), R80 (= R95), R101 (= R116), H128 (= H143), Q131 (= Q146), D224 (= D234), C264 (= C274), R292 (= R302)
binding phosphoric acid mono(formamide)ester: S50 (= S65), T51 (= T66), R52 (= R67), T53 (= T68), R101 (= R116), C264 (= C274), L265 (= L275), R292 (= R302)
binding norvaline: L123 (= L138), N160 (= N174), D224 (= D234), S228 (= S238), M229 (= M239)

7np0A Crystal structure of mycobacterium tuberculosis argf in complex with (4-nitrophenyl)boronic acid.
42% identity, 94% coverage: 14:314/319 of query aligns to 4:302/305 of 7np0A

query
sites
7np0A

K

R

H

H

F

F

L

L

D

R

L

D

S

D

R

D

L

L

E

S

A

P

K

A

D

E

L

Q

R

A

A

E

I

V

L

L

D

E

L

L

A

A

G

E

L

L

K

K

A

K

K

-

R

-

G

-

Q

D

K

P

A

V

K

S

E

R

Q

R

P

P

L

L

E

Q

G

G

-

P

K

R

V

G

L

V

A

A

M

V

V

I

F

F

D

D

Q

K

P

N

S

S

T

T

R

R

T

T

R

R

V

F

S

S

F

F

D

E

L

L

A

G

M

I

R

A

E

Q

L

L

G

G

E

H

T

A

I

V

M

V

L

V

T

-

G

-

Q

-

E

D

M

S

Q

G

L

L

G

G

R

R

G

D

E
|
E

T

T

I

L

A

Q

D

D

T

T

A

A

R

K

V
|
V

L

L

S

S

R

R

F
x
Y

V

V

D

D

A

A

I

I

V

V

I

W

R
|
R

I

T

L

F

N

G

H

Q

A

E

D

R

L

L

L

D

E

A

L

M

A

A

E

S

Y

V

A

A

E

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

K

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

L

L

L

Q

T

T

F

I

E

A

E

E

H

R

R

K

G

G

P

A

I

L

K

R

G

G

R

L

T

R

I

L

A

S

W

Y

T

F

G

G

D

D

-

G

S

A

N

N

V

M

L

A

A

H

S

S

W

L

I

L

E

L

A

G

R

V

R

T

L

A

D

G

F

I

T

H

L

V

N

T

V

V

A

A

C

A

P

P

A

E

E

G

L

F

S

L

P

P

A

D

P

P

E

S

L

V

V

R

A

A

F

A

A

A

N

E

A

R

Q

R

-

A

-

Q

-

D

-

T

G

G

K

A

R

S

L

V

N

T

V

V

L

T

R

A

D

D

P

A

F

H

E

A

A

A

V

A

R

A

G

G

A

A

D

D

A

V

V

L

I

V

S

T

D
|
D

C

T

W

W

V

T

S

S

M

M

G

G

D

Q

E

E

E

N

E

D

E

G

A

L

R

D

R

R

Q

V

A

K

L

P

L

F

S

R

P

P

Y

F

Q

Q

V

L

N

N

A

S

E

R

L

L

M

L

A

A

T

L

A

A

A

D

K

S

D

D

A

A

I

I

F

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

T

T

D

D

E

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

F

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

G

A

I

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L

L

A

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W

W

C

L

F

L

active site: R99 (= R116), H126 (= H143), Q129 (= Q146), D222 (= D234), C262 (= C274), R290 (= R302)
binding p-nitrophenylboronic acid: E80 (= E97), V88 (= V105), Y92 (≠ F109)

7novA Crystal structure of mycobacterium tuberculosis argf in complex with (4-methyl-3-nitrophenyl)boronic acid.
42% identity, 94% coverage: 14:314/319 of query aligns to 4:299/302 of 7novA

query
sites
7novA

K

R

H

H

F

F

L

L

D

R

L

D

S

D

R

D

L

L

E

S

A

P

K

A

D

E

L

Q

R

A

A

E

I

V

L

L

D

E

L

L

A

A

G

E

L

L

K

K

A

K

K

-

R

-

G

-

Q

D

K

P

A

V

K

S

E

R

Q

R

P

P

L

L

E

Q

G

G

-

P

K

R

V

G

L

V

A

A

M

V

V

I

F

F

D

D

Q

K

P

N

S

S

T

T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

D

E

L

L

A

G

M

I

R

A

E

Q

L

L

G

G

E

H

T

A

I

V

M

V

L

V

T

D

G

S

Q

-

E

-

M

-

Q

-

L

-

G

-

R

R

G

D

E
|
E

T

T

I

L

A

Q

D

D

T

T

A

A

R

K

V
|
V

L

L

S

S

R

R

F
x
Y

V

V

D

D

A

A

I

I

V

V

I

W

R
|
R

I

T

L

F

N

G

H

Q

A

E

D

R

L

L

L

D

E

A

L

M

A

A

E

S

Y

V

A

A

E

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

K

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

L

L

L

Q

T

T

F

I

E

A

E

E

H

R

R

K

G

G

P

A

I

L

K

R

G

G

R

L

T

R

I

L

A

S

W

Y

T

F

G

G

D

D

-

G

S

A

N

N

V

M

L

A

A

H

S

S

W

L

I

L

E

L

A

G

R

V

R

T

L

A

D

G

F

I

T

H

L

V

N

T

V

V

A

A

C

A

P

P

A

E

E

G

L

F

S

L

P

P

A

D

P

P

E

S

L

V

V

R

A

A

F

A

A

A

N

E

A

R

Q

R

-

A

-

Q

-

D

-

T

G

G

K

A

R

S

L

V

N

T

V

V

L

T

R

A

D

D

P

A

F

H

E

A

A

A

V

A

R

A

G

G

A

A

D

D

A

V

V

L

I

V

S

T

D
|
D

C

T

W

W

V

T

S

S

M

M

G

G

D

Q

E

E

E

N

E

D

E

G

A

L

R

D

R

R

Q

V

A

K

L

P

L

F

S

R

P

P

Y

F

Q

Q

V

L

N

N

A

S

E

R

L

L

M

L

A

A

T

L

A

A

A

D

K

S

D

D

A

A

I

I

F

V

M

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

T

T

D

D

E

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

F

W

D

D

E

E

A
|
A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

G

A

I

L

L

L

A

V

W

W

C

L

F

L

active site: R96 (= R116), H123 (= H143), Q126 (= Q146), D219 (= D234), C259 (= C274), R287 (= R302)
binding (4-methyl-3-nitro-phenyl)-oxidanyl-oxidanylidene-boron: R53 (= R67), F56 (≠ V70), E77 (= E97), V85 (= V105), Y89 (≠ F109), L260 (= L275), A284 (= A299), R287 (= R302)

7nnzB Crystal structure of mycobacterium tuberculosis argf in complex with 5-methyl-4-phenylthiazol-2-amine.
41% identity, 94% coverage: 14:314/319 of query aligns to 3:294/297 of 7nnzB

query
sites
7nnzB

K

R

H

H

F

F

L

L

D

R

L

D

S

D

R

D

L

L

E

S

A

P

K

A

D

E

L

Q

R

A

A

E

I

V

L

L

D

E

L

L

A

A

G

E

L

L

K

K

A

K

K

-

R

-

G

-

Q

D

K

P

A

V

K

S

E

R

Q

R

P

P

L

L

E

Q

G

G

-

P

K

R

V

G

L

V

A

A

M

V

V

I

F

F

D

D

Q

K

P

N

S

S

T
|
T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

D

E

L

L

A

G

M

I

R

A

E

Q

L

L

G

G

E

H

T

A

I

V

M

V

L

V

T

D

G

S

Q

G

E

S

M

T

Q

Q

L

L

G

G

R

R

G

D

E

E

T

T

I

L

A

Q

D

D

T

T

A

A

R

K

V

V

L

L

S

S

R

R

F

Y

V

V

D

D

A

A

I

I

V

V

I

W

R
|
R

I

T

L

F

N

G

H

Q

A

E

D

R

L

L

L

D

E

A

L

M

A

A

E

S

Y

V

A

A

E

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

K

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

L

L

L

Q

T

T

F

I

E

A

E

E

H

R

R

K

G

G

P

A

I

L

K

R

G

G

R

L

T

R

I

L

A

S

W

Y

T

F

G

G

D

D

-

G

S

A

N

N

V

M

L

A

A

H

S

S

W

L

I

L

E

L

A

G

R

V

R

T

L

A

D

G

F

I

T

H

L

V

N

T

V

V

A

A

C

A

P

P

A

E

E

G

L

F

S

L

P

P

A

D

P

P

E

S

L

V

V

R

A

A

F

A

A

A

N

E

A

R

Q

R

-

A

-

Q

-

D

-

T

G

G

K

A

R

S

L

V

N

T

V

V

L

T

R

A

D

D

P

A

F

H

E

A

A

A

V

A

R

A

G

G

A

A

D

D

A

V

V

L

I

V

S

T

D
|
D

C

T

W

W

V

T

S

-

M

-

G

-

D

-

E

-

A

-

R

-

R

R

Q

V

A

K

L

P

L

F

S

R

P

P

Y

F

Q

Q

V

L

N

N

A

S

E

R

L

L

M

L

A

A

T

L

A

A

A

D

K

S

D

D

A

A

I

I

F

V

M

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

V

I

T

T

D

D

E

A

V

V

I

M

D

D

G

G

P

P

Q

A

S

S

V

A

V

V

F

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

G

A

I

L

L

L

A

V

W

W

C

L

F

L

active site: R101 (= R116), H128 (= H143), Q131 (= Q146), D224 (= D234), C254 (= C274), R282 (= R302)
binding 5-methyl-4-phenyl-1,3-thiazol-2-amine: T51 (= T66), R52 (= R67), F55 (≠ V70)

Query Sequence

>WP_012383167.1 NCBI__GCF_000019845.1:WP_012383167.1
MTPLPVSDLIVQPKHFLDLSRLEAKDLRAILDLAAGLKAKRRRGQKAKEQPLEGKVLAMV
FDQPSTRTRVSFDLAMRELGGETIMLTGQEMQLGRGETIADTARVLSRFVDAIVIRILNH
ADLLELAEYAEVPVINGLTKLSHPCQIMADLLTFEEHRGPIKGRTIAWTGDSNNVLASWI
EAARRLDFTLNVACPAELSPAPELVAFANAQGKRLNVLRDPFEAVRGADAVISDCWVSMG
DEEEEARRQALLSPYQVNAELMATAAKDAIFMHCLPAHRGEEVTDEVIDGPQSVVFDEAE
NRLHAQKGILAWCFGVTGS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory