SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012383230.1 NCBI__GCF_000019845.1:WP_012383230.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 6 hits to proteins with known functional sites (download)

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
60% identity, 100% coverage: 1:201/201 of query aligns to 537:734/758 of O14289

query
sites
O14289

M

I

E

P

K

K

F

F

T

T

T

V

L

V

T

E

G

G

V

I

A

A

P

P

L

L

K

P

I

M

T

A

N

N

V

V

D

D

T

T

D

D

M

K

I

I

P

P

K

K

Q

Q

Y

F

L

L

K

K

T

T

I

I

K

K

R

R

T

T

G

G

L

L

G

G

E

Q

G

F

L

A

F

F

S

Y

E

E

L

I

R

R

F

Y

K

D

D

A

D

D

G

G

T

K

E

E

N

I

P

P

D

D

F

F

V

V

L

L

N

N

Q

R

P

E

A

P

Y

Y

R

R

K

H

A

A

S

T

I

V

L

L

V

V

A

A

G

H

D

D

N

N

F

F

G

G

C

C

G

G

S

S

R

R

E

E

H

H

A

A

P

P

W

W

A

A

L

L

L

N

D

D

F

F

G

G

I

I

R

R

C

V

V

I

I

I

S

A

T

P

S

S

F

F

A

A

D

D

I

I

F

F

Y

F

N

N

C

C

F

F

K

K

N

N

G

G

I

M

L

L

P

P

I

I

K

P

V

T

T

P

P

I

E

E

Q

Q

L

V

A

N

L

D

L

M

M

M

E

K

D

A

A

A

E

E

R

N

G

-

A

-

N

Q

A

V

T

K

L

F

T

S

V

V

D

D

L

L

E

V

N

N

Q

Q

T

T

I

I

R

T

G

Y

P

G

D

D

G

-

G

K

S

Q

I

V

P

K

F

F

N

D

I

V

D

E

P

P

F

F

R

R

K

K

H

H

C

C

L

L

L

V

N

N

G

G

L

L

D

D

I

I

G

G

L

L

T

T

M

L

Q

Q

K

K

A

E

D

T

E

M

I

I

S

D

A

A

Y

F

E

E

E

A

R

A

Q

R

A

E

K

E

Q

N

H

F

A

P

W

W

L

M

Sites not aligning to the query:

486 modified: Phosphoserine
488 modified: Phosphoserine

P9WK95 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
45% identity, 95% coverage: 1:191/201 of query aligns to 1:184/198 of P9WK95

query
sites
P9WK95

M

M

E

E

K

A

F

F

T

H

T

T

L

H

T

S

G

G

V

I

A

G

A

V

P

P

L

L

K

R

I

R

T

S

N

N

V

V

D

D

T

T

D

D

M

Q

I

I

P

P

K

A

Q

V

Y

F

L

L

K

K

T

R

I

V

K

T

R

R

T

T

G

G

L

F

G

E

E

D

G

G

L

L

F

F

S

A

E

G

L

W

R

R

F

-

K

-

D

-

G

-

T

-

E

S

N

D

P

P

D

A

F

F

V

V

L

L

N

N

Q

L

P

S

A

P

Y

F

R

D

K

R

A

G

S

S

I

V

L

L

V

V

A

A

G

G

D

P

N

D

F

F

G

G

C

T

G

G

S

S

R

R

E

E

H

H

A

A

P

V

W

W

A

A

L

L

L

M

D

D

F

Y

G

G

I

F

R

R

C

V

V

V

I

I

S

S

T

S

S

R

F

F

A

G

D

D

I

I

F

F

Y

R

N

G

N

N

C

A

F

G

K

K

N

A

G

G

I

L

L

L

P

A

I

A

K

E

V

V

T

A

P

Q

E

D

Q

D

L

V

A

E

L

L

M

W

E

K

D

L

A

I

E

E

R

Q

G

S

A

P

N

G

A

L

T

E

L

I

T

T

V

A

D

N

L

L

E

Q

N

D

Q

R

T

I

I

I

R

T

G

A

P

A

D

T

G

-

G

V

S

V

I

L

P

P

F

F

N
x
K

I

I

D

D

P

D

F

H

R

S

K

A

H

W

C

R

L

L

N

E

G

G

L

L

D

D

I

I

G

A

L

L

T

T

M

L

Q

R

K

K

A

L

D

D

E

E

I

I

S

E

A

A

Y

F

E

E

K154 (≠ N161) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Q58667 Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
39% identity, 54% coverage: 18:126/201 of query aligns to 13:110/170 of Q58667

query
sites
Q58667

N

D

V

V

D

D

T

T

D

D

M

A

I

I

P

P

K

G

Q

P

Y

Y

L

L

K
x
R

T
|
T

I

T

K

D

R

P

T

Y

G

E

L

L

G

A

E

S

G

H

L

C

F

M

S

A

E

G

L

I

R

-

F

-

K

-

D

-

G

-

T

D

E

E

N

N

-

F

P

P

D

K

F

K

V

V

L

-

N

-

Q

-

P

-

A

-

Y

-

R

K

K

E

A

G

S

D

I

V

L

I

V

V

A

A

G

G

D

E

N

N

F

F

G

G

C

C

G

G

S

S

R

R

E

E

H

Q

A

A

P

V

W

I

A

A

L

I

L

K

D

Y

F

C

G

G

I

I

R

K

C

A

V

V

I

I

S

A

T

K

S

S

F

F

A

A

D

R

I

I

F

F

Y

Y

N

R

N

N

C

A

F

I

K

N

N

V

G

G

I

L

L

I

P

P

I

I

K

I

V

A

T

N

P

T

E

D

Q

E

L

I

R26 (≠ K31) mutation R->K,V: Allows the recognition of substrates of IPMI enzymes since it becomes able to efficiently catalyze citraconate hydration and 3-isopropylmalate dehydration. Largely decreases substrate affinity for cis-homo(1-3)-aconitate.
T27 (= T32) mutation to A: Largely decreases substrate affinity for cis-homo(1-3)-aconitate while slightly increases activity on these substrates, and also decreases substrate affinity for maleate. Gains the ability to hydrate citraconate, an IPMI substrate.

2pkpA Crystal structure of 3-isopropylmalate dehydratase (leud)from methhanocaldococcus jannaschii dsm2661 (mj1271) (see paper)
39% identity, 54% coverage: 18:126/201 of query aligns to 13:110/167 of 2pkpA

query
sites
2pkpA

N
x
D

V

V

D

D

T

T

D

D

M

A

I

I

P

P

K

G

Q

P

Y

Y

L

L

K

R

T

T

I

T

K

D

R

P

T

Y

G

E

L

L

G

A

E

S

G

H

L

C

F

M

S

A

E

G

L

I

R

-

F

-

K

-

D

-

G

-

T

D

E

E

N

N

-

F

P

P

D

K

F

K

V

V

L

-

N

-

Q

-

P

-

A

-

Y

-

R

K

K

E

A

G

S

D

I

V

L

I

V

V

A

A

G

G

D

E

N

N

F

F

G

G

C
|
C

G

G

S

S

R

R

E

E

H

Q

A

A

P

V

W

I

A

A

L

I

L

K

D

Y

F

C

G

G

I

I

R

K

C

A

V

V

I

I

S

A

T

K

S

S

F

F

A

A

D

R

I

I

F

F

Y

Y

N

R

N

N

C

A

F

I

K

N

N

V

G

G

I

L

L

I

P

P

I

I

K

I

V

A

T

N

P

T

E

D

Q

E

L

I

binding zinc ion: D13 (≠ N18), C63 (= C79)

P20004 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Bos taurus (Bovine) (see 2 papers)
47% identity, 27% coverage: 65:119/201 of query aligns to 650:707/780 of P20004

query
sites
P20004

Y

Y

R

K

K

K

A

H

S

G

I

I

-

R

-

W

L

V

V

V

A

I

G

G

D

D

-

E

N

N

F

Y

G

G

C

E

G

G

S

S

S
|
S

R
|
R

E

E

H

H

A

A

P

A

W

L

A

E

L

P

L

R

D

H

F

L

G

G

I

G

R

R

C

A

V

I

I

I

S

T

T

K

S

S

F

F

A

A

D

R

I

I

F

H

Y

E

N

T

N

N

C

L

F

K

K

K

N

Q

G

G

I

L

L

L

P

P

I

L

SR 670:671 (= SR 82:83) binding substrate

Sites not aligning to the query:

99 binding substrate
192:194 binding substrate
385 binding [4Fe-4S] cluster
448 binding [4Fe-4S] cluster
451 binding [4Fe-4S] cluster
474 binding substrate
479 binding substrate
607 binding substrate

5acnA Structure of activated aconitase. Formation of the (4fe-4s) cluster in the crystal (see paper)
40% identity, 25% coverage: 70:119/201 of query aligns to 631:680/754 of 5acnA

query
sites
5acnA

I

V

L

V

V

I

A

G

G

D

D

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

S

S

R
|
R

E

E

H

H

A

S

P

A

W

L

A

E

L

P

L

R

D

H

F

L

G

G

I

G

R

R

C

A

V

I

I

I

S

T

T

K

S

S

F

F

A

A

D
x
R

I

I

F

H

Y

E

N

T

N

N

C

L

F

K

K

K

N

Q

G

G

I

L

L

L

P

P

I

L

active site: S642 (= S81), R644 (= R83)
binding tricarballylic acid: R666 (≠ D105)

Sites not aligning to the query:

active site: 100, 101, 165, 447
binding fe3-s4 cluster: 145, 147, 167, 358, 421, 424, 446
binding tricarballylic acid: 198, 235

Query Sequence

>WP_012383230.1 NCBI__GCF_000019845.1:WP_012383230.1
MEKFTTLTGVAAPLKITNVDTDMIIPKQYLKTIKRTGLGEGLFSELRFKDDGTENPDFVL
NQPAYRKASILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIK
VTPEQLALLMEDAERGANATLTVDLENQTIRGPDGGSIPFNIDPFRKHCLLNGLDDIGLT
MQKADEISAYEERQAKQHAWL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory