SitesBLAST
Comparing WP_012384994.1 NCBI__GCF_000019845.1:WP_012384994.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
56% identity, 98% coverage: 10:474/474 of query aligns to 5:465/465 of 3pm9A
- active site: A149 (= A156), L159 (≠ N166)
- binding flavin-adenine dinucleotide: P69 (≠ T74), Q70 (= Q75), G71 (= G76), G72 (= G77), N73 (= N78), T74 (= T79), G75 (= G80), L76 (= L81), G79 (= G84), Q80 (= Q85), L91 (= L98), L133 (= L140), G134 (≠ A141), A135 (≠ S142), C139 (= C146), T140 (= T147), G142 (= G149), G143 (= G150), S146 (≠ A153), T147 (= T154), A149 (= A156), G150 (= G157), E200 (= E207), G201 (= G208), I205 (= I212), I206 (= I213), E423 (= E432)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
37% identity, 91% coverage: 44:474/474 of query aligns to 45:464/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R336), T337 (≠ S340), K348 (= K351), Y379 (≠ F384), H381 (= H386), H388 (= H393), H423 (= H433)
- binding flavin-adenine dinucleotide: P75 (≠ T74), Q76 (= Q75), G77 (= G76), G78 (= G77), N79 (= N78), T80 (= T79), G81 (= G80), M82 (≠ L81), G85 (= G84), S86 (≠ Q85), L139 (= L140), G140 (≠ A141), A141 (≠ S142), C145 (= C146), G149 (= G150), N150 (= N151), A152 (= A153), T153 (= T154), G157 (= G158), G207 (= G208), I212 (= I213), E422 (= E432), N459 (= N469)
- binding zinc ion: H381 (= H386), H388 (= H393), E422 (= E432)
Sites not aligning to the query:
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
37% identity, 91% coverage: 44:474/474 of query aligns to 45:464/466 of 6lpuA
- binding flavin-adenine dinucleotide: P75 (≠ T74), G77 (= G76), G78 (= G77), N79 (= N78), T80 (= T79), G81 (= G80), G85 (= G84), S86 (≠ Q85), L139 (= L140), G140 (≠ A141), A141 (≠ S142), C145 (= C146), H146 (≠ T147), G148 (= G149), G149 (= G150), N150 (= N151), A152 (= A153), T153 (= T154), A155 (= A156), E206 (= E207), G207 (= G208), I211 (= I212), I212 (= I213), E422 (= E432), N459 (= N469)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R336), T337 (≠ S340), K348 (= K351), Y379 (≠ F384), H381 (= H386), H388 (= H393), H423 (= H433)
- binding zinc ion: H381 (= H386), H388 (= H393), E422 (= E432)
Sites not aligning to the query:
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
37% identity, 91% coverage: 44:474/474 of query aligns to 45:464/466 of 6lpqA
- binding flavin-adenine dinucleotide: P75 (≠ T74), G77 (= G76), G78 (= G77), N79 (= N78), T80 (= T79), G81 (= G80), G85 (= G84), S86 (≠ Q85), L139 (= L140), G140 (≠ A141), A141 (≠ S142), C145 (= C146), H146 (≠ T147), G149 (= G150), N150 (= N151), A152 (= A153), T153 (= T154), A155 (= A156), G157 (= G158), E206 (= E207), G207 (= G208), I211 (= I212), I212 (= I213), E422 (= E432), N459 (= N469)
- binding d-malate: M82 (≠ L81), R333 (= R336), T337 (≠ S340), K348 (= K351), Y379 (≠ F384), H381 (= H386), H388 (= H393), E422 (= E432), H423 (= H433)
- binding zinc ion: H381 (= H386), H388 (= H393), E422 (= E432)
Sites not aligning to the query:
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
37% identity, 91% coverage: 44:474/474 of query aligns to 45:464/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R336), T337 (≠ S340), K348 (= K351), Y379 (≠ F384), H381 (= H386), H388 (= H393), N390 (= N395), H423 (= H433)
- binding flavin-adenine dinucleotide: P75 (≠ T74), G77 (= G76), G78 (= G77), N79 (= N78), T80 (= T79), G81 (= G80), M82 (≠ L81), G85 (= G84), S86 (≠ Q85), L139 (= L140), G140 (≠ A141), A141 (≠ S142), C145 (= C146), G149 (= G150), N150 (= N151), A152 (= A153), T153 (= T154), A155 (= A156), G157 (= G158), G207 (= G208), I212 (= I213), E422 (= E432), H423 (= H433)
- binding zinc ion: H381 (= H386), H388 (= H393), E422 (= E432)
Sites not aligning to the query:
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
37% identity, 91% coverage: 44:474/474 of query aligns to 46:465/467 of 6lpnB
- binding flavin-adenine dinucleotide: P76 (≠ T74), G78 (= G76), G79 (= G77), N80 (= N78), T81 (= T79), G82 (= G80), M83 (≠ L81), G86 (= G84), S87 (≠ Q85), L140 (= L140), A142 (≠ S142), C146 (= C146), H147 (≠ T147), G150 (= G150), N151 (= N151), A153 (= A153), T154 (= T154), G208 (= G208), I212 (= I212), I213 (= I213), E423 (= E432), N460 (= N469)
Sites not aligning to the query:
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
37% identity, 91% coverage: 44:474/474 of query aligns to 98:517/521 of Q8N465
- S109 (≠ T55) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ V73) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G77) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ V95) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ L101) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ M120) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P137) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A153) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (= A179) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G181) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ S325) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R336) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (≠ S340) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ S349) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (= K351) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- R419 (≠ A369) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ G378) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H386) binding Zn(2+); mutation to A: Loss of catalytic activity.
- G436 (= G388) to V: slight reduction in catalytic activity
- N439 (= N391) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H393) binding Zn(2+); mutation to A: Loss of catalytic activity.
- N443 (= N395) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
- V444 (≠ I396) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ K404) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E432) binding Zn(2+); mutation to A: Loss of catalytic activity.
- H476 (= H433) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- G477 (= G434) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
33% identity, 98% coverage: 12:474/474 of query aligns to 32:496/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
33% identity, 93% coverage: 34:473/474 of query aligns to 33:463/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ T74), G75 (= G76), S76 (≠ G77), G77 (≠ N78), T78 (= T79), G79 (= G80), L80 (= L81), A83 (≠ G84), C84 (≠ Q85), P137 (≠ A141), G138 (≠ S142), E139 (= E143), A142 (≠ C146), T143 (= T147), G146 (= G150), N147 (= N151), S149 (≠ A153), T150 (= T154), A152 (= A156), G153 (= G157), E203 (= E207), G204 (= G208), I209 (= I213), E422 (= E432), H423 (= H433)
- binding fe (iii) ion: H377 (= H386), H384 (= H393), E422 (= E432)
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
32% identity, 98% coverage: 9:474/474 of query aligns to 3:455/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R336), W322 (vs. gap), H369 (= H386), H376 (= H393), H414 (= H433)
- binding flavin-adenine dinucleotide: P68 (≠ T74), G70 (= G76), T71 (≠ G77), G72 (≠ N78), T73 (= T79), G74 (= G80), G78 (= G84), V79 (≠ Q85), L90 (= L98), P132 (≠ L140), G133 (≠ A141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (= T154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (= I213), W322 (vs. gap), E413 (= E432), N450 (= N469)
- binding manganese (ii) ion: H369 (= H386), H376 (= H393), E413 (= E432)
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
32% identity, 98% coverage: 9:474/474 of query aligns to 3:454/454 of 8jdzA
- binding (3S)-3-methyl-2-oxopentanoic acid: R318 (≠ Q328), W322 (≠ F332), H369 (= H386), H376 (= H393), H413 (= H433)
- binding flavin-adenine dinucleotide: E32 (≠ H45), P68 (≠ T74), G70 (= G76), T71 (≠ G77), G72 (≠ N78), T73 (= T79), G74 (= G80), G78 (= G84), V79 (≠ Q85), L90 (= L98), P132 (≠ L140), G133 (≠ A141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (= T154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (= I213), W322 (≠ F332), E412 (= E432), H413 (= H433), N449 (= N469)
- binding manganese (ii) ion: H369 (= H386), H376 (= H393), E412 (= E432)
8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
32% identity, 98% coverage: 9:474/474 of query aligns to 3:454/454 of 8jdyA
- binding 2-oxo-4-methylpentanoic acid: R318 (≠ Q328), W322 (≠ F332), S336 (= S349), H369 (= H386), H376 (= H393), H413 (= H433)
- binding flavin-adenine dinucleotide: P68 (≠ T74), G70 (= G76), T71 (≠ G77), G72 (≠ N78), T73 (= T79), G74 (= G80), G78 (= G84), V79 (≠ Q85), L90 (= L98), P132 (≠ L140), G133 (≠ A141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (= T154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (= I213), E412 (= E432), N449 (= N469)
- binding manganese (ii) ion: H369 (= H386), H376 (= H393), E412 (= E432)
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
32% identity, 98% coverage: 9:474/474 of query aligns to 3:454/454 of 8jdvA
- binding 2-Ketohexanoic acid: V75 (≠ L81), R317 (≠ Q328), W321 (≠ F332), H368 (= H386), H375 (= H393), H413 (= H433)
- binding flavin-adenine dinucleotide: P68 (≠ T74), G70 (= G76), T71 (≠ G77), G72 (≠ N78), T73 (= T79), G74 (= G80), G78 (= G84), V79 (≠ Q85), L90 (= L98), P132 (≠ L140), G133 (≠ A141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (= T154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (= I213), W321 (≠ F332), Y322 (≠ W333), E412 (= E432), H413 (= H433), N449 (= N469)
- binding manganese (ii) ion: H368 (= H386), H375 (= H393), E412 (= E432)
8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
32% identity, 98% coverage: 9:474/474 of query aligns to 3:455/455 of 8jdeA
- binding flavin-adenine dinucleotide: P68 (≠ T74), G70 (= G76), T71 (≠ G77), G72 (≠ N78), T73 (= T79), G74 (= G80), G78 (= G84), V79 (≠ Q85), L90 (= L98), P132 (≠ L140), G133 (≠ A141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (= T154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (= I213), W322 (≠ F332), E413 (= E432), H414 (= H433), N450 (= N469)
- binding lactic acid: R318 (≠ Q328), H369 (= H386), H376 (= H393), H414 (= H433)
- binding manganese (ii) ion: H369 (= H386), H376 (= H393), E413 (= E432)
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
32% identity, 98% coverage: 9:474/474 of query aligns to 3:455/455 of 8jdxA
- binding flavin-adenine dinucleotide: E32 (≠ H45), P68 (≠ T74), G70 (= G76), T71 (≠ G77), G72 (≠ N78), T73 (= T79), G74 (= G80), G78 (= G84), V79 (≠ Q85), L90 (= L98), P132 (≠ L140), G133 (≠ A141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (= T154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (= I213), W322 (≠ F332), E413 (= E432), H414 (= H433), N450 (= N469)
- binding 3-methyl-2-oxobutanoic acid: R318 (≠ Q328), H369 (= H386), H376 (= H393), H414 (= H433)
- binding manganese (ii) ion: H369 (= H386), H376 (= H393), E413 (= E432)
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
32% identity, 98% coverage: 9:474/474 of query aligns to 3:456/456 of 8jdsA
- binding flavin-adenine dinucleotide: E32 (≠ H45), P68 (≠ T74), G70 (= G76), T71 (≠ G77), G72 (≠ N78), T73 (= T79), G74 (= G80), G78 (= G84), V79 (≠ Q85), L90 (= L98), P132 (≠ L140), G133 (≠ A141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (= T154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (= I213), W323 (≠ F332), E414 (= E432), H415 (= H433), N451 (= N469)
- binding manganese (ii) ion: H370 (= H386), H377 (= H393), E414 (= E432)
- binding pyruvic acid: R319 (≠ Q328), H370 (= H386), H377 (= H393), H415 (= H433)
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
32% identity, 98% coverage: 9:474/474 of query aligns to 3:455/455 of 8jdtA
- binding 2-ketobutyric acid: R318 (≠ Q328), H369 (= H386), H376 (= H393), H414 (= H433)
- binding flavin-adenine dinucleotide: P68 (≠ T74), G70 (= G76), T71 (≠ G77), G72 (≠ N78), T73 (= T79), G74 (= G80), G78 (= G84), V79 (≠ Q85), L90 (= L98), P132 (≠ L140), G133 (≠ A141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (= T154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (= I213), W322 (≠ F332), E413 (= E432), H414 (= H433), N450 (= N469)
- binding manganese (ii) ion: H369 (= H386), H376 (= H393), E413 (= E432)
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 98% coverage: 10:474/474 of query aligns to 3:456/459 of P9WIT1
- K354 (≠ D363) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
31% identity, 98% coverage: 9:474/474 of query aligns to 3:455/455 of 8jdpA
- binding flavin-adenine dinucleotide: P68 (≠ T74), G70 (= G76), T71 (≠ G77), G72 (≠ N78), T73 (= T79), G74 (= G80), G78 (= G84), V79 (≠ Q85), L90 (= L98), P132 (≠ L140), G133 (≠ A141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (= T154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (= I213), H369 (= H386), E413 (= E432), N450 (= N469)
- binding deaminohydroxyvaline: R319 (= R336), H414 (= H433)
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
32% identity, 98% coverage: 9:474/474 of query aligns to 3:456/456 of 8jdrA
- binding flavin-adenine dinucleotide: P68 (≠ T74), G70 (= G76), T71 (≠ G77), G72 (≠ N78), T73 (= T79), G74 (= G80), G78 (= G84), V79 (≠ Q85), L90 (= L98), P132 (≠ L140), G133 (≠ A141), A134 (≠ S142), G140 (= G150), M141 (≠ N151), A143 (= A153), T144 (= T154), A146 (= A156), S147 (≠ G157), E200 (= E207), G201 (= G208), I206 (= I213), Y324 (≠ W333), H370 (= H386), E414 (= E432), N451 (= N469)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (≠ Q328), W323 (≠ F332), H415 (= H433)
Query Sequence
>WP_012384994.1 NCBI__GCF_000019845.1:WP_012384994.1
MDTQSFSGEDLLSRLRLVLGEKHVLTEGVDMAAFLEEPRGLYHGHALAVVRPGSTQEVAS
VMTLCAEAQTNVVTQGGNTGLVGGQIPLAGTKPIVLSLTRLNQLREIDLPSETMTVEAGM
TLAAVQAAADTVERLFPLSLASEGSCTIGGNLATNAGGTNVIAYGNARALVLGLEVVLAD
GRILHDLSKLKKDNTGYDLKDLFIGSEGTLGIITAAVLKLFPKPRSRETAFIGLSSPRAA
VDFLTLARAEAGPNIVAFELIPRIGIDFVLAHSEGNRDPFNKQHAWYVLLELASPQKQGL
DTILLHVLEAGMEKGLIEDAAIAASLAQHEAFWRLRELLSEVQGREGGSIKHDVSVPIAA
VPDFLDDVANTLAKALPGSRPVPFGHLGDGNIHCNISQPIGADKQAFLDRWDEVNTLVHG
LVAKYHGSISAEHGIGQLKRKLLPQVKDPVALDVMRAVKTALDPQNLLNPGKVL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory