SitesBLAST
Comparing WP_012385382.1 NCBI__GCF_000019845.1:WP_012385382.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
48% identity, 97% coverage: 5:573/586 of query aligns to 8:581/596 of 1t9dA
- active site: Y29 (= Y26), G31 (= G28), G32 (= G29), A33 (= A30), I34 (≠ V31), E55 (= E52), T78 (= T75), F117 (= F114), Q118 (= Q115), E119 (= E116), K167 (= K164), R227 (≠ A225), M263 (= M261), V290 (≠ I288), V406 (= V397), L431 (= L422), G432 (= G423), M434 (= M425), D459 (≠ E450), N486 (= N477), E488 (≠ Y479), Q489 (≠ M480), M491 (= M482), V492 (= V483), W495 (= W486), L517 (= L509), G522 (= G514), L523 (≠ C515), K556 (≠ R548)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G29), A33 (= A30), V107 (= V104), P108 (= P105), F117 (= F114), K167 (= K164), M263 (= M261), D288 (= D286), R289 (= R287), W495 (= W486)
- binding flavin-adenine dinucleotide: R157 (= R154), G216 (= G213), A217 (≠ G214), G218 (= G215), N221 (= N218), T243 (= T241), L244 (= L242), Q245 (≠ M243), M260 (= M258), L261 (= L259), H264 (= H262), G283 (= G281), A284 (= A282), R285 (= R283), D287 (= D285), R289 (= R287), V290 (≠ I288), E316 (≠ D305), V317 (≠ I306), N321 (≠ S310), G334 (= G323), D335 (= D324), A336 (≠ C325), Q410 (= Q401), M411 (= M402), G429 (= G420), G430 (= G421)
- binding magnesium ion: D459 (≠ E450), N486 (= N477), E488 (≠ Y479)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E52), P81 (= P78), Q118 (= Q115), G432 (= G423), M434 (= M425), M464 (= M455)
1t9aA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
48% identity, 97% coverage: 5:573/586 of query aligns to 9:582/597 of 1t9aA
- active site: Y30 (= Y26), G32 (= G28), G33 (= G29), A34 (= A30), I35 (≠ V31), E56 (= E52), T79 (= T75), F118 (= F114), Q119 (= Q115), E120 (= E116), K168 (= K164), R228 (≠ A225), M264 (= M261), V291 (≠ I288), V407 (= V397), L432 (= L422), G433 (= G423), M435 (= M425), D460 (≠ E450), N487 (= N477), E489 (≠ Y479), Q490 (≠ M480), M492 (= M482), V493 (= V483), W496 (= W486), L518 (= L509), G523 (= G514), L524 (≠ C515), K557 (≠ R548)
- binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: G33 (= G29), V108 (= V104), P109 (= P105), F118 (= F114), K168 (= K164), M264 (= M261), D289 (= D286), R290 (= R287), M492 (= M482), V493 (= V483), W496 (= W486)
- binding flavin-adenine dinucleotide: R158 (= R154), G217 (= G213), A218 (≠ G214), G219 (= G215), N222 (= N218), T244 (= T241), L245 (= L242), Q246 (≠ M243), L262 (= L259), M264 (= M261), H265 (= H262), G284 (= G281), A285 (= A282), R286 (= R283), D288 (= D285), R290 (= R287), V291 (≠ I288), E317 (≠ D305), V318 (≠ I306), N322 (≠ S310), G335 (= G323), D336 (= D324), A337 (≠ C325), Q411 (= Q401), M412 (= M402), G430 (= G420), G431 (= G421)
- binding magnesium ion: D460 (≠ E450), N487 (= N477), E489 (≠ Y479)
- binding propyl trihydrogen diphosphate: V407 (= V397), G408 (= G398), Q409 (= Q399), H410 (= H400), M435 (= M425), G459 (= G449), D460 (≠ E450), A461 (= A451), S462 (= S452), N487 (= N477), E489 (≠ Y479), Q490 (≠ M480), G491 (= G481), M492 (= M482)
- binding 5-{[ethyl(methyl)amino]methyl}-2-methyl-5,6-dihydropyrimidin-4-amine: G433 (= G423), M435 (= M425), M465 (= M455)
5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
47% identity, 96% coverage: 7:566/586 of query aligns to 14:576/583 of 5k3sA
- active site: Y33 (= Y26), G35 (= G28), G36 (= G29), A37 (= A30), S38 (≠ V31), E59 (= E52), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (= E116), K171 (= K164), M266 (= M261), V293 (≠ I288), V400 (= V397), G426 (= G423), M428 (= M425), D453 (≠ E450), N480 (= N477), H482 (≠ Y479), L483 (≠ M480), M485 (= M482), V486 (= V483), W489 (= W486), H558 (≠ R548)
- binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R287), M485 (= M482), W489 (= W486), G569 (= G559)
- binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G213), G223 (= G214), G224 (= G215), T246 (= T241), L247 (= L242), M248 (= M243), L264 (= L259), M266 (= M261), G286 (= G281), R288 (= R283), D290 (= D285), V293 (≠ I288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (≠ C325), M405 (= M402), G423 (= G420)
- binding magnesium ion: D453 (≠ E450), N480 (= N477), H482 (≠ Y479)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V397), G401 (= G398), Q402 (= Q399), H403 (= H400), G426 (= G423), M428 (= M425), D453 (≠ E450), G454 (≠ A451), S455 (= S452), N480 (= N477), H482 (≠ Y479), L483 (≠ M480), G484 (= G481), M485 (= M482), V486 (= V483)
P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
47% identity, 96% coverage: 7:566/586 of query aligns to 99:661/670 of P17597
- A122 (= A30) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
- M124 (≠ L32) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
- E144 (= E52) binding thiamine diphosphate
- S186 (= S94) binding FAD
- P197 (= P105) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
- R199 (≠ H107) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
- Q207 (= Q115) binding thiamine diphosphate
- K220 (= K128) binding (R)-imazaquin
- R246 (= R154) binding (R)-imazaquin; binding FAD
- K256 (= K164) binding chlorimuron-ethyl
- G308 (= G214) binding FAD
- TL 331:332 (= TL 241:242) binding FAD
- C340 (≠ A250) modified: Cysteine sulfinic acid (-SO2H)
- LGMH 349:352 (= LGMH 259:262) binding FAD
- GVRFD 371:375 (≠ GARFD 281:285) binding FAD
- DR 376:377 (= DR 286:287) binding chlorimuron-ethyl
- DI 395:396 (= DI 305:306) binding FAD
- DV 414:415 (≠ DC 324:325) binding FAD
- QH 487:488 (= QH 399:400) binding thiamine diphosphate
- GG 508:509 (= GG 420:421) binding FAD
- GAM 511:513 (≠ GTM 423:425) binding thiamine diphosphate
- D538 (≠ E450) binding Mg(2+)
- DGS 538:540 (≠ EAS 450:452) binding thiamine diphosphate
- N565 (= N477) binding Mg(2+)
- NQHLGM 565:570 (≠ NQYMGM 477:482) binding thiamine diphosphate
- H567 (≠ Y479) binding Mg(2+)
- W574 (= W486) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
- S653 (= S558) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
47% identity, 96% coverage: 7:566/586 of query aligns to 14:576/582 of 8et4A
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V397), G401 (= G398), Q402 (= Q399), H403 (= H400), G426 (= G423), M428 (= M425), G452 (= G449), D453 (≠ E450), G454 (≠ A451), S455 (= S452), M458 (= M455), N480 (= N477), H482 (≠ Y479), L483 (≠ M480), G484 (= G481), M485 (= M482), V486 (= V483)
- binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G213), G223 (= G214), G224 (= G215), T246 (= T241), L247 (= L242), M248 (= M243), L264 (= L259), M266 (= M261), H267 (= H262), G286 (= G281), V287 (≠ A282), R288 (= R283), D290 (= D285), R292 (= R287), V293 (≠ I288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (≠ C325), M405 (= M402), G423 (= G420)
- binding magnesium ion: F370 (≠ Y366), D453 (≠ E450), M458 (= M455), Q461 (= Q458), N480 (= N477), H482 (≠ Y479), K533 (≠ P523)
- binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M261), R292 (= R287), M485 (= M482), W489 (= W486), S568 (= S558)
5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
47% identity, 96% coverage: 7:566/586 of query aligns to 14:576/582 of 5wj1A
- active site: Y33 (= Y26), G35 (= G28), G36 (= G29), A37 (= A30), S38 (≠ V31), E59 (= E52), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (= E116), K171 (= K164), M266 (= M261), V293 (≠ I288), V400 (= V397), G426 (= G423), M428 (= M425), D453 (≠ E450), N480 (= N477), H482 (≠ Y479), L483 (≠ M480), M485 (= M482), V486 (= V483), W489 (= W486), H558 (≠ R548)
- binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G213), G223 (= G214), G224 (= G215), T246 (= T241), L247 (= L242), M248 (= M243), M263 (= M258), L264 (= L259), G286 (= G281), R288 (= R283), V293 (≠ I288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (≠ C325), M405 (= M402), G423 (= G420), G424 (= G421)
- binding magnesium ion: D453 (≠ E450), N480 (= N477), H482 (≠ Y479)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M261), D291 (= D286), R292 (= R287), M485 (= M482), W489 (= W486), S568 (= S558)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V397), G401 (= G398), Q402 (= Q399), H403 (= H400), M428 (= M425), D453 (≠ E450), G454 (≠ A451), S455 (= S452), M458 (= M455), N480 (= N477), H482 (≠ Y479), L483 (≠ M480), G484 (= G481), M485 (= M482), V486 (= V483)
5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
47% identity, 96% coverage: 7:566/586 of query aligns to 14:576/582 of 5k6tA