SitesBLAST
Comparing WP_012385986.1 NCBI__GCF_000019845.1:WP_012385986.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q53TZ2 L-arabinose 1-dehydrogenase (NAD(P)(+)); D-galactose 1-dehydrogenase; EC 1.1.1.376; EC 1.1.1.120; EC 1.1.1.48 from Azospirillum brasilense (see paper)
58% identity, 94% coverage: 19:321/323 of query aligns to 5:307/309 of Q53TZ2
- D169 (= D183) mutation to A: Loss of activity.
- N173 (= N187) mutation to A: Decrease by 4 orders of magnitude in catalytic efficiency.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7cgqA Crystal structure of azospirillum brasilense l-arabinose 1- dehydrogenase e147a mutant (NADP and l-arabinose bound form)
57% identity, 94% coverage: 19:321/323 of query aligns to 2:304/306 of 7cgqA
- binding alpha-L-arabinopyranose: K88 (= K105), H116 (= H133), H150 (= H167), P167 (= P184), N170 (= N187)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G27), K11 (= K28), I12 (= I29), S34 (= S51), R35 (≠ H52), H36 (≠ D53), C64 (= C81), A65 (≠ T82), P66 (= P83), V69 (= V86), E87 (= E104), K88 (= K105), H116 (= H133), Q153 (= Q170), W155 (= W172), D166 (= D183), Y263 (= Y280)
5a06A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with sorbitol (see paper)
30% identity, 52% coverage: 22:190/323 of query aligns to 7:189/336 of 5a06A
- active site: K101 (= K105), Y186 (≠ N187)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L11 (≠ F26), G12 (= G27), Y13 (≠ K28), Y14 (≠ I29), S36 (= S51), G37 (≠ H52), T38 (≠ D53), K41 (vs. gap), Y59 (≠ F62), I77 (≠ C81), T78 (= T82), P79 (= P83), N80 (≠ P84), L82 (≠ V86), H83 (≠ R87), E100 (= E104), K101 (= K105), R129 (≠ H133), W168 (= W172), R169 (≠ I173), Y186 (≠ N187)
- binding sorbitol: D72 (= D76), H96 (= H100), K101 (= K105), R122 (= R126), R122 (= R126), L124 (= L128), F160 (≠ V163), R169 (≠ I173), D182 (= D183), Y186 (≠ N187)
Sites not aligning to the query:
5a03C Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
30% identity, 52% coverage: 22:190/323 of query aligns to 7:189/336 of 5a03C
- active site: K101 (= K105), Y186 (≠ N187)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G10 (= G25), L11 (≠ F26), G12 (= G27), Y13 (≠ K28), Y14 (≠ I29), S36 (= S51), G37 (≠ H52), T38 (≠ D53), K41 (vs. gap), Y59 (≠ F62), I77 (≠ C81), T78 (= T82), P79 (= P83), N80 (≠ P84), L82 (≠ V86), H83 (≠ R87), E100 (= E104), K101 (= K105), R129 (≠ H133), W168 (= W172), R169 (≠ I173), Y186 (≠ N187)
- binding beta-D-xylopyranose: K101 (= K105), F160 (≠ V163), R169 (≠ I173), D182 (= D183), Y186 (≠ N187)
Sites not aligning to the query:
5a05A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with maltotriose (see paper)
30% identity, 52% coverage: 22:190/323 of query aligns to 6:188/335 of 5a05A
- active site: K100 (= K105), Y185 (≠ N187)
- binding beta-D-glucopyranose: K100 (= K105), F159 (≠ V163), D181 (= D183), Y185 (≠ N187)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G25), L10 (≠ F26), G11 (= G27), Y12 (≠ K28), Y13 (≠ I29), S35 (= S51), G36 (≠ H52), T37 (≠ D53), K40 (vs. gap), Y58 (≠ F62), I76 (≠ C81), T77 (= T82), P78 (= P83), N79 (≠ P84), L81 (≠ V86), H82 (≠ R87), E99 (= E104), K100 (= K105), R128 (≠ H133), W167 (= W172), R168 (≠ I173), Y185 (≠ N187)
Sites not aligning to the query:
5a04A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glucose (see paper)
30% identity, 52% coverage: 22:190/323 of query aligns to 6:188/335 of 5a04A
- active site: K100 (= K105), Y185 (≠ N187)
- binding beta-D-glucopyranose: K100 (= K105), F159 (≠ V163), R168 (≠ I173), D181 (= D183), Y185 (≠ N187)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L10 (≠ F26), G11 (= G27), Y12 (≠ K28), Y13 (≠ I29), S35 (= S51), G36 (≠ H52), T37 (≠ D53), K40 (vs. gap), Y58 (≠ F62), I76 (≠ C81), T77 (= T82), P78 (= P83), N79 (≠ P84), L81 (≠ V86), H82 (≠ R87), E99 (= E104), K100 (= K105), R128 (≠ H133), W167 (= W172), R168 (≠ I173), Y185 (≠ N187)
Sites not aligning to the query:
5a03E Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
30% identity, 52% coverage: 22:190/323 of query aligns to 6:188/335 of 5a03E
- active site: K100 (= K105), Y185 (≠ N187)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G25), L10 (≠ F26), G11 (= G27), Y12 (≠ K28), Y13 (≠ I29), S35 (= S51), G36 (≠ H52), T37 (≠ D53), K40 (vs. gap), Y58 (≠ F62), I76 (≠ C81), T77 (= T82), P78 (= P83), N79 (≠ P84), H82 (≠ R87), E99 (= E104), K100 (= K105), R128 (≠ H133), W167 (= W172), R168 (≠ I173), Y185 (≠ N187)
- binding beta-D-xylopyranose: K100 (= K105), F159 (≠ V163), R168 (≠ I173), D181 (= D183), Y185 (≠ N187)
- binding alpha-D-xylopyranose: H134 (≠ A139)
Sites not aligning to the query:
5a02A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glycerol (see paper)
30% identity, 52% coverage: 22:190/323 of query aligns to 6:188/335 of 5a02A
- active site: K100 (= K105), Y185 (≠ N187)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: L10 (≠ F26), G11 (= G27), Y12 (≠ K28), Y13 (≠ I29), S35 (= S51), G36 (≠ H52), T37 (≠ D53), K40 (vs. gap), Y58 (≠ F62), I76 (≠ C81), T77 (= T82), P78 (= P83), L81 (≠ V86), H82 (≠ R87), E99 (= E104), K100 (= K105), R128 (≠ H133), W167 (= W172), R168 (≠ I173), Y185 (≠ N187)
Sites not aligning to the query:
1evjA Crystal structure of glucose-fructose oxidoreductase (gfor) delta1-22 s64d (see paper)
27% identity, 52% coverage: 22:190/323 of query aligns to 6:191/340 of 1evjA
- active site: K100 (= K105), Y188 (≠ N187)
- binding nicotinamide-adenine-dinucleotide: G9 (= G25), L10 (≠ F26), G11 (= G27), K12 (= K28), Y13 (≠ I29), D35 (= D53), L77 (≠ T82), P78 (= P83), N79 (≠ P84), H82 (≠ R87), E99 (= E104), K100 (= K105), R128 (≠ H133), W170 (= W172), R171 (≠ I173), Y188 (≠ N187)
Sites not aligning to the query:
2poqX Dimeric dihydrodiol dehydrogenase complexed with inhibitor, isoascorbic acid (see paper)
30% identity, 40% coverage: 69:196/323 of query aligns to 60:188/331 of 2poqX
Sites not aligning to the query:
2o4uX Crystal structure of mammalian dimeric dihydrodiol dehydrogenase (see paper)
30% identity, 40% coverage: 69:196/323 of query aligns to 60:188/331 of 2o4uX
Sites not aligning to the query:
- binding phosphate ion: 1, 2, 8, 9, 35, 36, 40, 57, 232, 234, 236, 247, 250, 253, 292, 296, 318
1rydA Crystal structure of glucose-fructose oxidoreductase from zymomonas mobilis
26% identity, 52% coverage: 22:190/323 of query aligns to 35:220/381 of 1rydA
- active site: K129 (= K105), Y217 (≠ N187)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L39 (≠ F26), G40 (= G27), K41 (= K28), Y42 (≠ I29), S64 (= S51), G65 (≠ H52), K69 (vs. gap), Y87 (≠ H67), L106 (≠ T82), P107 (= P83), N108 (≠ P84), L110 (≠ V86), H111 (≠ R87), E128 (= E104), K129 (= K105), R157 (≠ H133), A196 (≠ G169), W199 (= W172), R200 (≠ I173), Y217 (≠ N187)
Sites not aligning to the query:
1h6dA Oxidized precursor form of glucose-fructose oxidoreductase from zymomonas mobilis complexed with glycerol (see paper)
26% identity, 52% coverage: 22:190/323 of query aligns to 37:222/383 of 1h6dA
- active site: K131 (= K105), Y219 (≠ N187)
- binding glycerol: K131 (= K105), R202 (≠ I173), D215 (= D183), Y219 (≠ N187)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G40 (= G25), L41 (≠ F26), G42 (= G27), K43 (= K28), Y44 (≠ I29), S66 (= S51), G67 (≠ H52), K71 (vs. gap), Y89 (≠ H67), I107 (≠ C81), L108 (≠ T82), P109 (= P83), N110 (≠ P84), H113 (≠ R87), E130 (= E104), K131 (= K105), R159 (≠ H133), A198 (≠ G169), W201 (= W172), R202 (≠ I173), Y219 (≠ N187)
Sites not aligning to the query:
Q7JK39 Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; D-xylose 1-dehydrogenase; D-xylose-NADP dehydrogenase; Dimeric dihydrodiol dehydrogenase; Jmo2DD; EC 1.3.1.20; EC 1.1.1.179 from Macaca fuscata fuscata (Japanese macaque) (see paper)
30% identity, 40% coverage: 69:196/323 of query aligns to 61:189/334 of Q7JK39
- H79 (≠ R87) mutation to E: Decrease in K(d) and K(m) value for NADPH. Elimination of the fluorescence-energy transfer and enhancement of NADPH fluorescence by the binary complex formation. Potent inhibition of the dehydrogenase activity by high ionic strength.
- Y180 (≠ N187) mutation to F: Significant loss of activity. No effect on the high affinity for NADPH, fluorescence-energy transfer and enhancement of NADPH fluorescence by the binary complex formation.
Q9TQS6 Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; Cmo2DD; D-xylose 1-dehydrogenase; D-xylose-NADP dehydrogenase; Dimeric dihydrodiol dehydrogenase; EC 1.3.1.20; EC 1.1.1.179 from Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) (see paper)
30% identity, 40% coverage: 69:196/323 of query aligns to 61:189/334 of Q9TQS6
- R148 (≠ T156) mutation to A: No effect on activity. Reduced activity and exhibits significant temperature sensitivity; when associated with A-202.
Sites not aligning to the query:
- 202 R→A: No effect on activity. Reduced activity and exhibits significant temperature sensitivity; when associated with A-148.
2glxA Crystal structure analysis of bacterial 1,5-af reductase (see paper)
32% identity, 40% coverage: 20:149/323 of query aligns to 2:137/332 of 2glxA
- active site: K93 (= K105)
- binding acetate ion: K93 (= K105)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G25), A8 (≠ F26), S9 (≠ G27), T10 (≠ K28), I11 (= I29), S32 (≠ A48), T33 (≠ I49), R37 (≠ D53), S69 (≠ C81), T70 (= T82), N72 (≠ P84), H75 (≠ R87), E92 (= E104), K93 (= K105), H121 (= H133)
Sites not aligning to the query:
Q2I8V6 1,5-anhydro-D-fructose reductase; Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming); EC 1.1.1.292 from Ensifer adhaerens (Sinorhizobium morelense) (see paper)
32% identity, 40% coverage: 20:149/323 of query aligns to 3:138/333 of Q2I8V6
- ASTI 9:12 (≠ FGKI 26:29) binding NADP(+)
- S10 (≠ G27) mutation to G: Almost no effect.
- A13 (= A30) mutation to G: Can use NAD as cosubstrate as well as NADP.
- S33 (≠ A48) mutation to D: No activity.
- ST 33:34 (≠ AI 48:49) binding NADP(+)
- R38 (≠ D53) binding NADP(+)
- TTNELH 71:76 (≠ TPPQVR 82:87) binding NADP(+)
- EK 93:94 (= EK 104:105) binding NADP(+)
- K94 (= K105) mutation to G: Less than 1% remaining activity.
- N120 (≠ T131) binding NADP(+)
Sites not aligning to the query:
- 162:163 binding NADP(+)
- 176 D→A: Less than 1% remaining activity.
- 180 H→A: Less than 2% remaining activity.
- 206 G→I: No effect.
- 283 binding NADP(+)
1zh8A Crystal structure of oxidoreductase (tm0312) from thermotoga maritima at 2.50 a resolution
24% identity, 90% coverage: 19:308/323 of query aligns to 4:304/325 of 1zh8A
- active site: K98 (= K105), H187 (≠ N187)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G25), C11 (≠ F26), G12 (= G27), I13 (≠ K28), A14 (≠ I29), S37 (= S51), R38 (≠ H52), T39 (≠ D53), H42 (vs. gap), T74 (≠ C81), L75 (≠ T82), P76 (= P83), L79 (≠ V86), E97 (= E104), K98 (= K105), N126 (≠ H133), Y165 (≠ I165), W170 (= W172), R171 (≠ I173), H187 (≠ N187), Y276 (= Y280)
6jw8A The crystal structure of kand2 in complex with nadh and 3"-deamino-3"- hydroxykanamycin b (see paper)
33% identity, 41% coverage: 18:149/323 of query aligns to 1:138/342 of 6jw8A
- binding (2S,3R,4S,5S,6R)-2-[(1S,2S,3R,4S,6R)-3-[(2R,3R,4R,5S,6R)-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol: F11 (≠ K28)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G10 (= G27), F11 (≠ K28), M12 (≠ I29), D33 (≠ S51), L34 (≠ H52), T70 (≠ C81), T71 (= T82), P72 (= P83), N73 (≠ P84), L75 (≠ V86), H76 (≠ R87), Q79 (= Q90), E93 (= E104), K94 (= K105), N122 (≠ H133)
Sites not aligning to the query:
- binding (2S,3R,4S,5S,6R)-2-[(1S,2S,3R,4S,6R)-3-[(2R,3R,4R,5S,6R)-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol: 152, 154, 175, 176, 179, 236, 271
- binding 1,4-dihydronicotinamide adenine dinucleotide: 161, 179, 290
6jw7A The crystal structure of kand2 in complex with nadh and 3"-deamino-3"- hydroxykanamycin a (see paper)
33% identity, 41% coverage: 18:149/323 of query aligns to 1:138/342 of 6jw7A
- binding (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-bis(azanyl)-3-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2-oxidanyl-cyclohexyl]oxy-oxane-3,4,5-triol: F11 (≠ K28)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G25), G10 (= G27), F11 (≠ K28), M12 (≠ I29), D33 (≠ S51), L34 (≠ H52), T70 (≠ C81), T71 (= T82), P72 (= P83), N73 (≠ P84), L75 (≠ V86), H76 (≠ R87), Q79 (= Q90), E93 (= E104), K94 (= K105), N122 (≠ H133)
Sites not aligning to the query:
- binding (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-bis(azanyl)-3-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2-oxidanyl-cyclohexyl]oxy-oxane-3,4,5-triol: 154, 175, 179, 236, 271
- binding 1,4-dihydronicotinamide adenine dinucleotide: 161, 290
Query Sequence
>WP_012385986.1 NCBI__GCF_000019845.1:WP_012385986.1
MIQVLQPGPAAREETVPPIRLAIVGFGKIARDQHVPAIATTPGIALTAIASHDGGLEGIP
YFKTLDHLLSEGPQIDAVALCTPPQVRRAQAEVALAAGKHVLLEKPPGATVSEIAPLVAQ
AQRAGRTLFATWHSRFAPAVEPARAFLAERTLRAVTIAWKEDVRIWHPGQAWIWEPGGLG
VFDPGINALSILTEILPRPIFLTRAELAFPANRAAPIAADLDFSDTNGLAIHAEFDFRQT
GPQSWDILVETADGVLVLSKGGAELAHDGKKLVETPEAEYRGIYRRFVELIAGGMSDVDL
VPLQHVADAFMLGRRTLVEPFED
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory