SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012386429.1 NCBI__GCF_000019845.1:WP_012386429.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q56YA5 Serine--glyoxylate aminotransferase; Alanine--glyoxylate aminotransferase; AGT; Asparagine aminotransferase; Serine--pyruvate aminotransferase; EC 2.6.1.45; EC 2.6.1.44; EC 2.6.1.-; EC 2.6.1.51 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
46% identity, 94% coverage: 6:381/398 of query aligns to 8:385/401 of Q56YA5

query
sites
Q56YA5

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TGT 68:70 (≠ SGT 65:67) binding pyridoxal 5'-phosphate
T148 (= T145) binding pyridoxal 5'-phosphate
QK 200:201 (= QK 197:198) binding pyridoxal 5'-phosphate
K201 (= K198) binding 3-hydroxypyruvate
P251 (= P247) mutation to L: Abolishes aminotransferase activity.
R347 (= R343) binding 3-hydroxypyruvate

6pk1A Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana in presence of serine (see paper)
46% identity, 94% coverage: 6:381/398 of query aligns to 6:383/399 of 6pk1A

query
sites
6pk1A

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binding 3-hydroxypyruvic acid: F93 (= F92), R345 (= R343)
binding pyridoxal-5'-phosphate: T66 (≠ S65), G67 (= G66), T68 (= T67), W71 (= W70), F93 (= F92), T146 (= T145), D173 (= D172), V175 (≠ I174), K199 (= K198)

6pk3B Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana (see paper)
46% identity, 94% coverage: 6:381/398 of query aligns to 7:384/400 of 6pk3B

query
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6pk3B

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binding pyridoxal-5'-phosphate: T67 (≠ S65), G68 (= G66), T69 (= T67), W72 (= W70), F94 (= F92), T147 (= T145), D174 (= D172), V176 (≠ I174), Q199 (= Q197), K200 (= K198)

3islA Crystal structure of ureidoglycine-glyoxylate aminotransferase (pucg) from bacillus subtilis
30% identity, 93% coverage: 10:381/398 of query aligns to 10:380/387 of 3islA

query
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3islA

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L

A

E

G

C

D

E

W

W

R

G

H

T

G

V

A

F

D

D

P

P

A

E

A

D

I

I

E

I

A

R

K

E

L

I

A

K

E

K

D

V

K

K

P

P

K

K

S

-

I

-

K

-

A

I

V

V

M

A

V

M

V

V

H

H

N

G

E

E

T
|
T

S

S

T

T

G

G

V

R

I

I

S

H

R

P

I

L

A

K

E

A

I

I

R

G

K

E

A

A

I

C

D

R

K

T

A

E

N

D

H

-

P

-

A

A

L

L

L

F

M

I

V

V

D
|
D

T

A

I
x
V

S

A

S

T

L

I

G

G

S

G

A

C

D

E

Y

V

R

K

H

V

E

D

E

E

W

W

G

K

V

I

D

D

V

A

T

A

V

I

S

G

C

G

S

T

Q
|
Q

K
|
K

G

C

L

L

M

S

L

V

P

P

P

S

G

G

L

M

G

A

F

P

T

I

A

T

I

Y

S

N

D

E

K

R

A

V

-

A

-

D

L

V

A

I

A

A

S

A

K

R

T

N

N

R

K

P

M

I

P

T

R

S

S

N

Y

Y

W

F

D

D

W

L

E

S

E

Q

M

L

L

E

K

D

P

Y

-

W

N

S

A

E

N

R

G

R

F

L

F

N

P

H

Y

H

T

T

P

E

A

A

T

T

N

T

L

M

L

L

Y

Y

G

A

L

L

R

R

E

E

A

G

I

V

K

R

I

L

L

V

L

L

E

E

G

G

L

L

D

E

T

T

V

R

F

F

A

E

R

R

H

H

Q

R

H

H

L

H

A

E

A

A

T

L

R

A

A

A

A

G

V

I

R

K

A

A

W

M

G

G

L

L

E

R

I

L

L

F

C

G

L

D

E

D

P

S

A

C

E

K

Y

M

S

-

P

P

V

V

L

V

T

T

A

C

I

V

L

E

M

I

P

P

Q

G

G

G

H

I

D

D

A

G

D

E

Q

S

F

V

R

R

A

D

L

M

V

L

L

L

E

A

K

Q

Y

F

N

G

M

I

S

E

L

I

G

A

A

S

G

S

L

F

T

G

N

P

L

L

A

A

G

G

K

K

V

I

F

W

R

R

I

I

G

G

H

T

L

M

G

G

-

Y

E

S

C

C

N

R

E

K

L

E

T

N

L

V

I

L

G

F

T

V

L

L

G

A

G

G

V

L

E

E

M

A

G

V

L

L

A

L

A

R

A

H

G

N

V

A

P

G

H

I

K

E

A

A

G

G

G

K

V

A

L

L

A

Q

A

A

binding pyridoxal-5'-phosphate: T66 (= T67), S67 (vs. gap), R68 (vs. gap), F93 (= F92), T143 (= T145), D168 (= D172), V170 (≠ I174), Q193 (= Q197), K194 (= K198)

O32148 (S)-ureidoglycine--glyoxylate transaminase; UGXT; (S)-ureidoglycine--glyoxylate aminotransferase; Purine catabolism protein PucG; EC 2.6.1.112 from Bacillus subtilis (strain 168) (see paper)
30% identity, 93% coverage: 10:381/398 of query aligns to 14:402/416 of O32148

query
sites
O32148

L

I

Q

M

I

T

P

P

G

G

P

P

S

V

P

E

V

V

P

D

E

P

R

R

I

V

L

L

R

R

A

V

L

M

S

S

M

T

Q

P

V

V

I

V

D

G

H
x
Q

R

F

G

D

P

P

E

A

F

F

G

T

A

G

L

I

G

M

R

N

E

E

V

T

L

M

A

E

G

M

C

L

Q

R

T

E

I

L

F

F

K

Q

T

T

S

K

G

N

P

R

V

W

V

A

I

-

Y

Y

P

P

S

I

S

D

G

G

T

T

-

S

-

R

G

A

A

G

W

I

E

E

A

A

A

V

I

L

V

A

N

S

T

V

L

I

S

E

P

P

G

E

D

D

K

D

V

V

L

L

M

I

V

P

E

I

T

Y

G

G

H

R

F

F

A

G

T

Y

L

L

W

L

R

T

K

E

M

I

A

A

G

E

R

R

W

Y

G

G

L

A

D

N

V

V

D

H

F

M

L

L

A

E

G

C

D

E

W

W

R

G

H

T

G

V

A

F

D

D

P

P

A

E

A

D

I

I

E

I

A

R

K

E

L

I

A

K

E

K

D

V

K

K

P

P

K

K

S

-

I

-

K

-

A

I

V

V

M

A

V

M

V

V

H

H

N

G

E

E

T

T

S

S

T

T

G

G

V

R

I

I

S

H

R

P

I

L

A

K

E

A

I

I

R

G

K

E

A

A

I

C

D

R

K

T

A

E

N

D

H

-

P

-

A

A

L

L

L

F

M

I

V

V

D

D

T

A

I

V

S

A

S

T

L

I

G

G

S

G

A

C

D

E

Y

V

R

K

H

V

E

D

E

E

W

W

G

K

V

I

D

D

V

A

T

A

V

I

S

G

C

G

S

T

Q

Q

K
|
K

G

C

L

L

M

S

L

V

P

P

P

S

G

G

L

M

-

A

-

P

-

I

-

T

-

Y

-

N

-

E

-

R

-

V

-

A

-

D

-

V

-

I

-

A

-

R

-

K

-

V

-

E

-

R

G

G

F

I

T

A

A

T

I

Q

S

A

D

D

K

R

A

A

L

-

A

A

A

L

S

S

K

G

T

N

N

R

K

P

M

I

P

T

R

S

S

N

Y

Y

W

F

D

D

W

L

E

S

E

Q

M

L

L

E

K

D

P

Y

-

W

N

S

A

E

N

R

G

R

F

L

F
x
N

P

H

Y

H

T

T

P

E

A

A

T

T

N

T

L

M

L

L

Y

Y

G

A

L

L

R

R

E

E

A

G

I

V

K

R

I

L

L

V

L

L

E

E

G

G

L

L

D

E

T

T

V

R

F

F

A

E

R

R

H

H

Q

R

H

H

L

H

A

E

A

A

T

L

R

A

A

A

A

G

V

I

R

K

A

A

W

M

G

G

L

L

E

R

I

L

L

F

C

G

L

D

E

D

P

S

A

C

E

K

Y

M

S

-

P

P

V

V

L

V

T

T

A

C

I

V

L

E

M

I

P

P

Q

G

G

G

H

I

D

D

A

G

D

E

Q

S

F

V

R

R

A

D

L

M

V

L

L

L

E

A

K

Q

Y

F

N

G

M

I

S

E

L

I

G

A

A

S

G

S

L

F

T

G

N

P

L

L

A

A

G

G

K

K

V

I

F

W

R

R

I

I

G

G

H

T

L

M

G

G

-

Y

E

S

C

C

N

R

E

K

L

E

T

N

L

V

I

L

G

F

T

V

L

L

G

A

G

G

V

L

E

E

M

A

G

V

L

L

A

L

A

R

A

H

G

N

V

A

P

G

H

I

K

E

A

A

G

G

G

K

V

A

L

L

A

Q

A

A

Q37 (≠ H33) mutation to H: 5-fold decrease in transamination activity.
K198 (= K198) modified: N6-(pyridoxal phosphate)lysine
N264 (≠ F243) mutation to S: 9-fold decrease in transamination activity.; mutation to Y: Loss of transamination activity.

1vjoA Crystal structure of alanine--glyoxylate aminotransferase (alr1004) from nostoc sp. At 1.70 a resolution (see paper)
32% identity, 84% coverage: 13:348/398 of query aligns to 24:357/377 of 1vjoA

query
sites
1vjoA

P

P

G

G

P

P

S

S

P

N

V

A

P

H

E

P

R

S

I

V

L

L

R

Q

A

A

L

M

S

N

M

V

Q

S

V

P

I

V

D

G

H

H

R

L

G

D

P

P

E

A

F

F

G

L

A

A

L

L

G

M

R

D

E

E

V

I

L

Q

A

S

G

L

C

L

Q

R

T

Y

I

V

F

W

K

Q

T

T

S

E

G

N

P

P

V

L

V

T

I

I

Y

A

P

V

S

S

-

G

S
x
T

G
|
G

T
|
T

G

A

A

A

W

M

E

E

A

A

A

T

I

I

V

A

N

N

T

A

L

V

S

E

P

P

G

G

D

D

K

V

V

V

L

L

M

I

V

G

E

V

T

A

G

G

H

Y

F
|
F

A

G

T

N

L

R

W

L

R

V

K

D

M

M

A

A

G

G

R

R

W

Y

G

G

L

A

D

D

V

V

D

R

F

T

L

I

A

S

G

K

D

P

W

W

R

G

H

E

G

V

A

F

D

S

P

L

A

E

I

L

E

R

A

T

K

A

L

L

A

E

E

T

D

H

K

R

P

P

K

A

S

I

I

L

K

A

A

-

V

-

M

-

V

L

V

V

H

H

N

A

E

E

T
|
T

S

S

T

T

G

G

V

A

I

R

S

Q

R

P

I

L

A

E

E

G

I

V

R

G

K

E

A

L

I

C

D

R

K

E

A

F

N

G

H

-

P

-

A

T

L

L

M

L

V

V

D
|
D

T

T

I
x
V

S

T

S

S

L

L

G

G

S

G

A

V

D

P

Y

I

R

F

H

L

E

D

E

A

W

W

G

G

V

V

D

D

V

L

T

A

V

Y

S

S

C

C

S

S

Q

Q

K
|
K

G

G

L

L

M

G

L

C

P

S

P

P

G

G

L

A

G

S

F

P

T

F

A

T

I

M

S

S

D

S

K

R

A

A

L

I

A

E

A

K

S

L

K

Q

T

R

N

R

K

R

M

T

P

K

R

V

S

A

Y

N

W

W

D

Y

W

L

E

D

E

M

M

N

L

L

K

L

P

G

N

K

A

Y

N

W

G

G

-

S

-

E

-

R

F

V

F

Y

P

H

Y

H

T

T

P

A

A

P

T

I

N

N

L

L

L

Y

Y

Y

G

A

L

L

R

R

E

E

A

A

I

L

K

R

I

L

L

I

L

A

E

Q

E

E

G

G

L

L

D

A

T

N

V

C

F

W

A

Q

R

R

H

H

Q

Q

H

K

L

N

A

V

A

E

A

Y

T

L

R

W

A

E

A

R

V

L

R

E

A

D

W

I

G

G

L

L

E

S

I

-

L

L

C

H

L

V

E

E

P

K

A

E

E

Y

Y

R

S

L

P

P

V

T

L

L

T

T

A

T

I

V

L

C

M

I

P

P

Q

D

G

G

H

V

D

D

A

G

D

K

Q

A

F

V

R

A

A

R

L

R

V

L

L

L

E

N

K

E

Y

H

N

N

M

I

S

E

L

V

G

G

A

G

G

G

L

L

T

G

N

E

L

L

A

A

G

G

K

K

V

V

F

W

R

R

I

V

G

G

H

L

L

M

G

G

binding pyridoxal-5'-phosphate: T77 (≠ S65), G78 (= G66), T79 (= T67), F104 (= F92), T154 (= T145), D179 (= D172), V181 (≠ I174), K205 (= K198)

2dr1A Crystal structure of the ph1308 protein from pyrococcus horikoshii ot3
31% identity, 86% coverage: 7:348/398 of query aligns to 16:354/381 of 2dr1A

query
sites
2dr1A

R

K

H

Y

F

K

L

L

Q

F

I

T

P

A

G

G

P

P

S

V

P

A

V

C

P

F

E

P

R

E

I

V

L

L

R

E

A

I

L

M

S

K

M

V

Q

Q

V

M

I

F

D

S

H

H

R

R

G

S

P

K

E

E

F

Y

G

R

A

K

L

V

G

H

R

M

E

D

V

T

L

V

A

E

G

R

C

L

Q

R

T

E

I

F

F

L

K

E

T

V

-

E

S

K

G

G

P

E

V

V

V

L

I

L

Y

V

P

P

S

S

S
|
S

G
|
G

T
|
T

G

G

A

I

W

M

E

E

A

A

A

S

I

I

V

R

N

N

T

G

L

V

S

S

P

K

G

G

D

G

K

K

V

V

L

L

M

V

V

T

E

I

T

I

G

G

H

A

F
|
F

A

G

T

K

L

R

W

Y

R

K

K

E

M

V

A

V

G

E

R

S

W

N

G

G

L

R

D

K

V

A

D

V

F

V

L

L

A

E

G

Y

D

E

W

P

R

G

H

K

G

A

A

V

D

K

P

P

A

E

A

D

I

L

E

D

A

D

K

A

L

L

A

R

E

-

D

-

K

K

P

N

K

P

S

D

I

V

K

E

A

A

V

V

M

T

V

I

V

T

H

Y

N

N

E

E

T
|
T

S

S

T

T

G

G

V

V

I

L

S

N

R

P

I

L

A

P

E

E

I

L

R

A

K

K

A

V

I

A

D

-

K

-

A

K

N

E

H

H

P

D

A

K

L

L

L

V

M

F

V

V

D
|
D

T

A

I
x
V

S

S

S

A

L

M

G

G

S

G

A

A

D

D

Y

I

R

K

H

F

E

D

E

K

W

W

G

G

V

L

D

D

V

V

T

V

V

F

S

S

C
x
S

S

S

Q

Q

K
|
K

G

A

L

F

M

G

L

V

P

P

G

G

L

L

G

A

F

I

T

G

A

A

I

F

S

S

D

E

K

R

A

F

L

L

A

E

A

I

S

A

K

-

T

-

N

E

K

K

M

M

P

P

R

E

S

R

Y

G

W

W

D

Y

W

F

E

D

-

I

-

P

-

L

-

Y

-

V

E

K

M

Y

L

L

K

K

P

E

N

K

A

E

N

S

G

T

F

-

P

P

Y

S

T

T

P

P

A

P

T

M

N

P

L

Q

L

V

Y

F

G

G

L

I

R

N

E

V

A

A

I

L

K

R

I

I

L

I

L

E

E

K

E

M

-

G

G

G

L

K

D

E

T

K

V

W

F

L

A

E

R

M

H

Y

Q

E

H

K

L

R

A

A

A

K

A

M

T

V

R

R

A

E

A

G

V

V

R

R

A

E

W

I

G

G

L

L

E

D

I

I

L

L

C

A

L

-

E

E

P

P

A

G

E

H

Y

E

S

S

P

P

V

T

L

I

T

T

A

A

I

V

L

L

M

T

P

P

Q

P

G

G

H

I

D

K

A

G

D

D

Q

E

F

V

R

Y

A

E

L

-

V

A

L

M

E

R

K

K

Y

R

N

G

M

F

S

E

L

L

G

A

A

K

G

G

L

Y

T

G

N

S

L

V

A

K

G

E

K

K

V

T

F

F

R

R

I

I

G

G

H

H

L

M

G

G

binding pyridoxal-5'-phosphate: S75 (= S65), G76 (= G66), T77 (= T67), F102 (= F92), T153 (= T145), D178 (= D172), V180 (≠ I174), S201 (≠ C195), K204 (= K198)

2huuA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with alanine (see paper)
31% identity, 86% coverage: 9:349/398 of query aligns to 21:362/385 of 2huuA

query
sites
2huuA

F

L

L

L

Q

M

I

G

P
|
P

G

G

P

P

S

S

P

N

V

A

P

P

E

Q

R

R

I

V

L

L

R

D

A

A

L

M

S

S

M

R

Q

P

V

I

I

L

D

G

H
|
H

R

L

G

H

P

P

E

E

F

T

G

L

A

K

L

I

G

M

R

D

E

D

V

I

L

K

A

E

G

G

C

V

Q

R

T

Y

I

L

F

F

K

Q

T

T

S

N

G

N

-

I

P

A

V

T

V

F

I

C

Y

L

P

S

S

A

S

S

G

G

T

H

G

G

A

G

W

M

E

E

A

A

A

T

I

L

V

C

N

N

T

L

L

L

S

E

P

D

G

G

D

D

K

V

V

I

L

L

M

I

V

G

E

H

T

T

G

G

H

H

F

W

A

G

T

D

L

R

W

S

R

A

K

D

M

M

A

A

G

T

R

R

W

Y

G

G

L

A

D

D

V

V

D

R

F

V

L

V

A

K

G

S

D

K

W

V

R

G

H

Q

G

S

A

L

D

S

P

L

A

D

A

E

I

I

E

R

A

D

K

A

L

L

A

L

E

I

D

H

K

K

P

P

K

S

S

-

I

-

K

-

A

V

V

L

M

F

V

L

V

T

H

Q

N

G

E

D

T
x
S

S

S

T

T

G

G

V

V

I

L

S

Q

R

G

I

L

A

E

E

G

I

V

R

G

K

A

A

L

I

C

D

H

K

Q

A

-

N

-

H

H

P

N

A

C

L

L

M

I

V

V

D

D

T

T

I

V

S

A

S

S

L

L

G

G

S

G

A

A

D

P

Y

M

R

F

H

M

E

D

E

R

W

W

G

E

V

I

D

D

V

A

T

M

V

Y

S

T

C

G

S

S

Q

Q

K
|
K

G

V

L

L

M

G

L

A

P

P

G

G

L

I

G

T

F

P

T

V

A

S

I

F

S

S

D

H

K

R

A

A

L

V

A

E

A

R

S

Y

K

K

-

R

T

N

N

T

K

K

M

V

P

K

R

V

S

Y

Y

Y

W

W

D

D

-

M

-

S

-

L

-

V

-

G

-

D

-

Y

W

W

E

G

E

C

M

F

L

G

K

R

P

P

N

R

A

-

N

-

G

-

F

I

F
x
Y

P

H

Y

H

T

T

P

I

A

S

T

S

N

T

L

L

Y

Y

G

G

L

L

R

R

E

E

A

A

I

I

K

A

I

M

L

A

L

C

E

E

G

G

L

L

D

P

T

A

V

L

F

I

A

A

R

R

H

H

Q

E

H

D

L

C

A

A

A

K

A

R

T

L

R

Y

A

R

A

G

V

L

R

Q

A

D

W

A

G

G

L

F

E

E

I

L

L

Y

C

A

L

-

E

D

P

P

A

K

E

D

Y

R

S

L

P

S

V

T

L

V

T

T

A

T

I

I

L

K

M

V

P

P

Q

Q

G

G

H

V

D

D

A

W

D

L

Q

K

F

A

R

A

A

Q

L

Y

V

A

L

M

E

K

K

T

Y

Y

N

L

M

V

S

E

L

I

G

S

A

G

G

G

L
|
L

T

G

N

P

L

T

A

A

G

G

K

Q

V

V

F

F

R
|
R

I

I

G

G

H

L

L

M

G

G

E

Q

binding 1-butanol: H45 (= H33), Y257 (≠ F243)
binding alanine: P25 (= P13), S155 (≠ T145), K206 (= K198), L347 (= L334), R356 (= R343)

2huiA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with glyoxylic acid (see paper)
31% identity, 86% coverage: 9:349/398 of query aligns to 21:362/385 of 2huiA

query
sites
2huiA

F

L

L

L

Q

M

I

G

P
|
P

G

G

P

P

S

S

P

N

V

A

P

P

E

Q

R

R

I

V

L

L

R

D

A

A

L

M

S

S

M

R

Q

P

V

I

I

L

D

G

H

H

R

L

G

H

P

P

E

E

F

T

G

L

A

K

L

I

G

M

R

D

E

D

V

I

L

K

A

E

G

G

C

V

Q

R

T

Y

I

L

F

F

K

Q

T

T

S

N

G

N

-

I

P

A

V

T

V

F

I

C

Y

L

P

S

S

A

S

S

G

G

T

H

G

G

A

G

W

M

E

E

A

A

A

T

I

L

V

C

N

N

T

L

L

L

S

E

P

D

G

G

D

D

K

V

V

I

L

L

M

I

V

G

E

H

T

T

G

G

H

H

F

W

A

G

T

D

L

R

W

S

R

A

K

D

M

M

A

A

G

T

R

R

W

Y

G

G

L

A

D

D

V

V

D

R

F

V

L

V

A

K

G

S

D

K

W

V

R

G

H

Q

G

S

A

L

D

S

P

L

A

D

A

E

I

I

E

R

A

D

K

A

L

L

A

L

E

I

D

H

K

K

P

P

K

S

S

-

I

-

K

-

A

V

V

L

M

F

V

L

V

T

H

Q

N

G

E

D

T
x
S

S

S

T

T

G

G

V

V

I

L

S

Q

R

G

I

L

A

E

E

G

I

V

R

G

K

A

A

L

I

C

D

H

K

Q

A

-

N

-

H

H

P

N

A

C

L

L

M

I

V

V

D

D

T

T

I

V

S

A

S

S

L

L

G

G

S

G

A

A

D

P

Y

M

R

F

H

M

E

D

E

R

W

W

G

E

V

I

D

D

V

A

T

M

V

Y

S

T

C

G

S

S

Q

Q

K
|
K

G

V

L

L

M

G

L

A

P

P

G

G

L

I

G

T

F

P

T

V

A

S

I

F

S

S

D

H

K

R

A

A

L

V

A

E

A

R

S

Y

K

K

-

R

T

N

N

T

K

K

M

V

P

K

R

V

S

Y

Y

Y

W

W

D

D

-

M

-

S

-

L

-

V

-

G

-

D

-

Y

W

W

E

G

E

C

M

F

L

G

K

R

P

P

N

R

A

-

N

-

G

-

F

I

F

Y

P

H

Y

H

T

T

P

I

A

S

T

S

N

T

L

L

Y

Y

G

G

L

L

R

R

E

E

A

A

I

I

K

A

I

M

L

A

L

C

E

E

G

G

L

L

D

P

T

A

V

L

F

I

A

A

R

R

H

H

Q

E

H

D

L

C

A

A

A

K

A

R

T

L

R

Y

A

R

A

G

V

L

R

Q

A

D

W

A

G

G

L

F

E

E

I

L

L

Y

C

A

L

-

E

D

P

P

A

K

E

D

Y

R

S

L

P

S

V

T

L

V

T

T

A

T

I

I

L

K

M

V

P

P

Q

Q

G

G

H

V

D

D

A

W

D

L

Q

K

F

A

R

A

A

Q

L

Y

V

A

L

M

E

K

K

T

Y

Y

N

L

M

V

S

E

L

I

G

S

A

G

G

G

L

L

T

G

N

P

L

T

A

A

G

G

K

Q

V

V

F

F

R
|
R

I

I

G

G

H

L

L

M

G

G

E

Q

binding glyoxylic acid: P25 (= P13), S155 (≠ T145), K206 (= K198), R356 (= R343)

2hufA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase (see paper)
31% identity, 86% coverage: 9:349/398 of query aligns to 21:362/385 of 2hufA

query
sites
2hufA

F

L

L

L

Q

M

I

G

P

P

G

G

P

P

S

S

P

N

V

A

P

P

E

Q

R

R

I

V

L

L

R

D

A

A

L

M

S

S

M

R

Q

P

V

I

I

L

D

G

H
|
H

R

L

G

H

P

P

E

E

F

T

G

L

A

K

L

I

G

M

R

D

E

D

V

I

L

K

A

E

G

G

C

V

Q

R

T

Y

I

L

F

F

K

Q

T

T

S

N

G

N

-

I

P

A

V

T

V

F

I

C

Y

L

P

S

S

A

S

S

G

G

T

H

G

G

A

G

W

M

E

E

A

A

A

T

I

L

V

C

N

N

T

L

L

L

S

E

P

D

G

G

D

D

K

V

V

I

L

L

M

I

V

G

E

H

T

T

G

G

H

H

F

W

A

G

T

D

L

R

W

S

R

A

K

D

M

M

A

A

G

T

R

R

W

Y

G

G

L

A

D

D

V

V

D

R

F

V

L

V

A

K

G

S

D

K

W

V

R

G

H

Q

G

S

A

L

D

S

P

L

A

D

A

E

I

I

E

R

A

D

K

A

L

L

A

L

E

I

D

H

K

K

P

P

K

S

S

-

I

-

K

-

A

V

V

L

M

F

V

L

V

T

H

Q

N

G

E

D

T
x
S

S

S

T

T

G

G

V

V

I

L

S

Q

R

G

I

L

A

E

E

G

I

V

R

G

K

A

A

L

I

C

D

H

K

Q

A

-

N

-

H

H

P

N

A

C

L

L

M

I

V

V

D

D

T

T

I

V

S

A

S

S

L

L

G

G

S

G

A

A

D

P

Y

M

R

F

H

M

E

D

E

R

W

W

G

E

V

I

D

D

V

A

T

M

V

Y

S

T

C

G

S

S

Q

Q

K

K

G

V

L

L

M

G

L

A

P

P

G

G

L

I

G

T

F

P

T

V

A

S

I

F

S

S

D

H

K

R

A

A

L

V

A

E

A

R

S

Y

K

K

-

R

T

N

N

T

K

K

M

V

P

K

R

V

S

Y

Y

Y

W

W

D

D

-

M

-

S

-

L

-

V

-

G

-

D

-

Y

W

W

E

G

E

C

M

F

L

G

K

R

P

P

N

R

A

-

N

-

G

-

F

I

F
x
Y

P

H

Y

H

T

T

P

I

A

S

T

S

N

T

L

L

Y

Y

G

G

L

L

R

R

E

E

A

A

I

I

K

A

I

M

L

A

L

C

E

E

G

G

L

L

D

P

T

A

V

L

F

I

A

A

R

R

H

H

Q

E

H

D

L

C

A

A

A

K

A

R

T

L

R

Y

A

R

A

G

V

L

R

Q

A

D

W

A

G

G

L

F

E

E

I

L

L

Y

C

A

L

-

E

D

P

P

A

K

E

D

Y

R

S

L

P

S

V

T

L

V

T

T

A

T

I

I

L

K

M

V

P

P

Q

Q

G

G

H

V

D

D

A

W

D

L

Q

K

F

A

R

A

A

Q

L

Y

V

A

L

M

E

K

K

T

Y

Y

N

L

M

V

S

E

L

I

G

S

A

G

G

G

L

L

T

G

N

P

L

T

A

A

G

G

K

Q

V

V

F

F

R
|
R

I

I

G

G

H

L

L

M

G

G

E

Q

binding 1-butanol: H45 (= H33), S155 (≠ T145), Y257 (≠ F243), R356 (= R343)

Q3LSM4 Alanine--glyoxylate aminotransferase; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (see paper)
31% identity, 86% coverage: 9:349/398 of query aligns to 21:362/393 of Q3LSM4

query
sites
Q3LSM4

F

L

L

L

Q

M

I

G

P

P

G

G

P

P

S

S

P

N

V

A

P

P

E

Q

R

R

I

V

L

L

R

D

A

A

L

M

S

S

M

R

Q

P

V

I

I

L

D

G

H

H

R

L

G

H

P

P

E

E

F

T

G

L

A

K

L

I

G

M

R

D

E

D

V

I

L

K

A

E

G

G

C

V

Q

R

T

Y

I

L

F

F

K

Q

T

T

S

N

G

N

-

I

P

A

V

T

V

F

I

C

Y

L

P

S

S

A

S
|
S

G
|
G

T
x
H

G

G

A

G

W

M

E

E

A

A

A

T

I

L

V

C

N

N

T

L

L

L

S

E

P

D

G

G

D

D

K

V

V

I

L

L

M

I

V

G

E

H

T

T

G

G

H

H

F

W

A

G

T

D

L

R

W

S

R

A

K

D

M

M

A

A

G

T

R

R

W

Y

G

G

L

A

D

D

V

V

D

R

F

V

L

V

A

K

G

S

D

K

W

V

R

G

H

Q

G

S

A

L

D

S

P

L

A

D

A

E

I

I

E

R

A

D

K

A

L

L

A

L

E

I

D

H

K

K

P

P

K

S

S

-

I

-

K

-

A

V

V

L

M

F

V

L

V

T

H

Q

N

G

E

D

T
x
S

S

S

T

T

G

G

V

V

I

L

S

Q

R

G

I

L

A

E

E

G

I

V

R

G

K

A

A

L

I

C

D

H

K

Q

A

-

N

-

H

H

P

N

A

C

L

L

M

I

V

V

D

D

T

T

I

V

S

A

S

S

L

L

G

G

S

G

A

A

D

P

Y

M

R

F

H

M

E

D

E

R

W

W

G

E

V

I

D

D

V

A

T

M

V

Y

S

T

C

G

S

S

Q
|
Q

K
|
K

G

V

L

L

M

G

L

A

P

P

G

G

L

I

G

T

F

P

T

V

A

S

I

F

S

S

D

H

K

R

A

A

L

V

A

E

A

R

S

Y

K

K

-

R

T

N

N

T

K

K

M

V

P

K

R

V

S

Y

Y

Y

W

W

D

D

-

M

-

S

-

L

-

V

-

G

-

D

-

Y

W

W

E

G

E

C

M

F

L

G

K

R

P

P

N

R

A

-

N

-

G

-

F

I

F
x
Y

P

H

Y

H

T
|
T

P

I

A

S

T

S

N

T

L

L

Y

Y

G

G

L

L

R

R

E

E

A

A

I

I

K

A

I

M

L

A

L

C

E

E

G

G

L

L

D

P

T

A

V

L

F

I

A

A

R

R

H

H

Q

E

H

D

L

C

A

A

A

K

A

R

T

L

R

Y

A

R

A

G

V

L

R

Q

A

D

W

A

G

G

L

F

E

E

I

L

L

Y

C

A

L

-

E

D

P

P

A

K

E

D

Y

R

S

L

P

S

V

T

L

V

T

T

A

T

I

I

L

K

M

V

P

P

Q

Q

G

G

H

V

D

D

A

W

D

L

Q

K

F

A

R

A

A

Q

L

Y

V

A

L

M

E

K

K

T

Y

Y

N

L

M

V

S

E

L

I

G

S

A

G

G

G

L

L

T

G

N

P

L

T

A

A

G

G

K

Q

V

V

F

F

R
|
R

I

I

G

G

H

L

L

M

G

G

E

Q

SGH 78:80 (≠ SGT 65:67) binding in other chain
S155 (≠ T145) binding glyoxylate; binding L-alanine
Q205 (= Q197) binding in other chain
K206 (= K198) modified: N6-(pyridoxal phosphate)lysine
Y257 (≠ F243) binding pyridoxal 5'-phosphate
T260 (= T246) binding pyridoxal 5'-phosphate
R356 (= R343) binding glyoxylate

1j04A Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro (see paper)
30% identity, 85% coverage: 13:351/398 of query aligns to 25:364/387 of 1j04A

query
sites
1j04A

P
|
P

G

G

P

P

S

S

P

N

V

L

P

P

E

P

R

R

I

I

L

M

R

A

A

A

L

G

S

G

M

L

Q

Q

V

M

I

I

D

G

H

S

R

M

G

S

P

K

E

D

F

M

G

Y

A

Q

L

I

G

M

R

D

E

E

V

I

L

K

A

E

G

G

C

I

Q

Q

T

Y

I

V

F

F

K

Q

T

T

S

R

G

N

P

P

V

L

V

T

I

L

Y

V

P

I

S

S

-

G

S

S

G

G

T

H

G

C

A

A

W

L

E

E

A

A

I

L

V

V

N

N

T

V

L

L

S

E

P

P

G

G

D

D

K

S

V

F

L

L

M

V

V

G

E

A

T

N

G

G

H

I

F

W

A

G

T

Q

L

R

W

A

R

V

K

D

M

I

A

G

G

E

R

R

W

I

G

G

L

A

D

R

V

V

D

H

F

P

L

M

A

T

G

K

D

D

W

P

R

G

H

G

G

H

A

Y

D

T

P

L

A

Q

A

E

I

V

E

E

A

E

K

G

L

L

A

A

E

Q

D

H

K

K

P

P

K

V

S

-

I

-

K

-

A

L

V

L

M

F

V

L

V

T

H

H

N

G

E

E

T

S

S

S

T

T

G

G

V

V

I

L

S

Q

R

P

I

L

A

D

E

G

I

F

R

R

K

E

A

L

I

C

D

H

K

R

A

-

N

-

H

Y

P

K

A

C

L

L

M

L

V

V

D

D

T

S

I

V

S

A

S

S

L

L

G

G

S

G

A

T

D

P

Y

L

R

Y

H

M

E

D

E

R

W

Q

G

G

V

I

D

D

V

I

T

L

V

Y

S

S

C

G

S

S

Q

Q

K

K

G

A

L

L

M

N

L

A

P

P

G

G

L

T

G

S

F

L

T

I

A

S

I

F

S

S

D

D

K

K

A

A

-

K

L

M

A

Y

A

S

S

R

K

K

T

T

N

K

-

P

-

F

-

S

-

F

-

Y

-

L

-

D

-

I

K

K

M

W

P

L

R

A

S

N

Y

F

W

W

D

G

W

C

E

D

M

Q

L

P

K

R

P

-

N

-

A

-

N

-

G

-

F

M

F

Y

P

H

Y

H

T

T

P

I

A

P

T

V

N

I

L

S

L

L

Y

Y

G

S

L

L

R

R

E

E

A

S

I

L

K

A

I

L

L

I

L

A

E

E

E

Q

G

G

L

L

D

E

T

N

V

S

F

W

A

R

R

Q

H

H

Q

R

H

E

L

A

A

A

A

Y

T

L

R

H

A

G

A

R

V

L

R

Q

A

A

W

L

G

G

L

L

E

Q

I

L

L

F

C

V

L

K

E

D

P

P

A

A

E

L

Y

R

S

L

P

P

V

T

L

V

T

T

A

T

I

V

L

A

M

V

P

P

Q

A

G

G

H

Y

D

D

A

W

D

R

Q

D

F

I

R

V

A

S

L

Y

V

V

L

I

E

D

K

H

Y

F

N

D

M

I

S

E

L

I

G

M

A

G

G

G

L

L

T

G

N

P

L

S

A

T

G

G

K

K

V

V

F

L

R
|
R

I

I

G

G

H

L

L

L

G

G

E

-

C

C

N

N

binding (aminooxy)acetic acid: P25 (= P13), R357 (= R343)

6rv0A Human alanine:glyoxylate aminotransferase major allele (agt-ma); with pmp in the active site (see paper)
30% identity, 85% coverage: 13:351/398 of query aligns to 23:362/384 of 6rv0A

query
sites
6rv0A

P

P

G

G

P

P

S

S

P

N

V

L

P

P

E

P

R

R

I

I

L

M

R

A

A

A

L

G

S

G

M

L

Q

Q

V

M

I

I

D

G

H

S

R

M

G

S

P

K

E

D

F

M

G

Y

A

Q

L

I

G

M

R

D

E

E

V

I

L

K

A

E

G

G

C

I

Q

Q

T

Y

I

V

F

F

K

Q

T

T

S

R

G

N

P

P

V

L

V

T

I

L

Y

V

P

I

S

S

-

G

S
|
S

G
|
G

T
x
H

G

C

A

A

W

L

E

E

A

A

I

L

V

V

N

N

T

V

L

L

S

E

P

P

G

G

D

D

K

S

V

F

L

L

M

V

V

G

E

A

T

N

G

G

H

I

F
x
W

A

G

T

Q

L

R

W

A

R

V

K

D

M

I

A

G

G

E

R

R

W

I

G

G

L

A

D

R

V

V

D

H

F

P

L

M

A

T

G

K

D

D

W

P

R

G

H

G

G

H

A

Y

D

T

P

L

A

Q

A

E

I

V

E

E

A

E

K

G

L

L

A

A

E

Q

D

H

K

K

P

P

K

V

S

-

I

-

K

-

A

L

V

L

M

F

V

L

V

T

H

H

N

G

E

E

T

S

S

S

T

T

G

G

V

V

I

L

S

Q

R

P

I

L

A

D

E

G

I

F

R

G

K

E

A

L

I

C

D

H

K

R

A

-

N

-

H

Y

P

K

A

C

L

L

M

L

V

V

D
|
D

T

S

I
x
V

S
x
A

S

S

L

L

G

G

S

G

A

T

D

P

Y

L

R

Y

H

M

E

D

E

R

W

Q

G

G

V

I

D

D

V

I

T

L

V

Y

S

S

C

G

S

S

Q

Q

K
|
K

G

A

L

L

M

N

L

A

P

P

G

G

L

T

G

S

F

L

T

I

A

S

I

F

S

S

D

D

K

K

A

A

-

K

L

M

A

Y

A

S

S

R

K

K

T

T

N

K

-

P

-

F

-

S

-

F

-

Y

-

L

-

D

-

I

K

K

M

W

P

L

R

A

S

N

Y

F

W

W

D

G

W

C

E

D

M

Q

L

P

K

R

P

-

N

-

A

-

N

-

G

-

F

M

F

Y

P

H

Y

H

T

T

P

I

A

P

T

V

N

I

L

S

L

L

Y

Y

G

S

L

L

R

R

E

E

A

S

I

L

K

A

I

L

L

I

L

A

E

E

E

Q

G

G

L

L

D

E

T

N

V

S

F

W

A

R

R

Q

H

H

Q

R

H

E

L

A

A

A

A

Y

T

L

R

H

A

G

A

R

V

L

R

Q

A

A

W

L

G

G

L

L

E

Q

I

L

L

F

C

V

L

K

E

D

P

P

A

A

E

L

Y

R

S

L

P

P

V

T

L

V

T

T

A

T

I

V

L

A

M

V

P

P

Q

A

G

G

H

Y

D

D

A

W

D

R

Q

D

F

I

R

V

A

S

L

Y

V

V

L

I

E

D

K

H

Y

F

N

D

M

I

S

E

L

I

G

M

A

G

G

G

L

L

T

G

N

P

L

S

A

T

G

G

K

K

V

V

F

L

R

R

I

I

G

G

H

L

L

L

G

G

E

-

C

C

N

N

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S76 (= S65), G77 (= G66), H78 (≠ T67), W103 (≠ F92), D178 (= D172), V180 (≠ I174), A181 (≠ S175), K204 (= K198)

5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
30% identity, 85% coverage: 13:351/398 of query aligns to 23:362/385 of 5hhyA

query
sites
5hhyA

P

P

G

G

P

P

S

S

P

N

V

L

P

P

E

P

R

R

I

I

L

M

R

A

A

A

L

G

S

G

M

L

Q

Q

V

M

I

I

D

G

H

S

R

M

G

S

P

K

E

D

F

M

G

Y

A

Q

L

I

G

M

R

D

E

E

V

I

L

K

A

E

G

G

C

I

Q

Q

T

Y

I

V

F

F

K

Q

T

T

S

R

G

N

P

P

V

L

V

T

I

L

Y

V

P

I

S

S

-

G

S
|
S

G
|
G

T
x
H

G

C

A

A

W

L

E

E

A

A

I

L

V

V

N

N

T

V

L

L

S

E

P

P

G

G

D

D

K

S

V

F

L

L

M

V

V

G

E

A

T

N

G

G

H

I

F
x
W

A

G

T

Q

L

R

W

A

R

V

K

D

M

I

A

G

G

E

R

R

W

I

G

G

L

A

D

R

V

V

D

H

F

P

L

M

A

T

G

K

D

D

W

P

R

G

H

G

G

H

A

Y

D

T

P

L

A

Q

A

E

I

V

E

E

A

E

K

G

L

L

A

A

E

Q

D

H

K

K

P

P

K

V

S

-

I

-

K

-

A

L

V

L

M

F

V

L

V

T

H

H

N

G

E

E

T
x
S

S

S

T

T

G

G

V

V

I

L

S

Q

R

P

I

L

A

D

E

G

I

F

R

G

K

E

A

L

I

C

D

H

K

R

A

-

N

-

H

Y

P

K

A

C

L

L

M

L

V

V

D
|
D

T

S

I
x
V

S

A

S

S

L

L

G

G

S

G

A

T

D

P

Y

L

R

Y

H

M

E

D

E

R

W

Q

G

G

V

I

D

D

V

I

T

L

V

Y

S

S

C

G

S

S

Q
|
Q

K
|
K

G

A

L

L

M

N

L

A

P

P

G

G

L

T

G

S

F

L

T

I

A

S

I

F

S

S

D

D

K

K

A

A

-

K

L

M

A

Y

A

S

S

R

K

K

T

T

N

K

-

P

-

F

-

S

-

F

-

Y

-

L

-

D

-

I

K

K

M

W

P

L

R

A

S

N

Y

F

W

W

D

G

W

C

E

D

M

Q

L

P

K

R

P

-

N

-

A

-

N

-

G

-

F

M

F
x
Y

P

H

Y

H

T
|
T

P

I

A

P

T

V

N

I

L

S

L

L

Y

Y

G

S

L

L

R

R

E

E

A

S

I

L

K

A

I

L

L

I

L

A

E

E

E

Q

G

G

L

L

D

E

T

N

V

S

F

W

A

R

R

Q

H

H

Q

R

H

E

L

A

A

A

A

Y

T

L

R

H

A

G

A

R

V

L

R

Q

A

A

W

L

G

G

L

L

E

Q

I

L

L

F

C

V

L

K

E

D

P

P

A

A

E

L

Y

R

S

L

P

P

V

T

L

V

T

T

A

T

I

V

L

A

M

V

P

P

Q

A

G

G

H

Y

D

D

A

W

D

R

Q

D

F

I

R

V

A

S

L

Y

V

V

L

I

E

D

K

H

Y

F

N

D

M

I

S

E

L

I

G

M

A

G

G

G

L

L

T

G

N

P

L

S

A

T

G

G

K

K

V

V

F

L

R

R

I

I

G

G

H

L

L

L

G

G

E

-

C

C

N

N

binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S76 (= S65), G77 (= G66), H78 (≠ T67), W103 (≠ F92), S153 (≠ T145), D178 (= D172), V180 (≠ I174), Q203 (= Q197), K204 (= K198), Y255 (≠ F243), T258 (= T246)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
30% identity, 85% coverage: 13:351/398 of query aligns to 28:367/392 of P21549

query
sites
P21549

P

P

G

G

P

P

S

S

P

N

V

L

P

P

E

P

R
|
R

I

I

L

M

R

A

A

A

L
x
G

S

G

M

L

Q

Q

V

M

I

I

D
x
G

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A

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I

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A

E

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G

C

I

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T

Y

I

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F

K

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T

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R

G

N

P

P

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L

V

T

I

L

Y

V

P

I

S

S

-

G

S

S

G
|
G

T

H

G

C

A

A

W

L

E

E

A

A

I

L

V

V

N

N

T

V

L

L

S

E

P

P

G

G

D

D

K

S

V

F

L

L

M

V

V

G

E

A

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N

G

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x
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A

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A

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-

I

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L

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E

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I

F

R
x
G

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E

A

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C

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N

-

H

Y

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A

C

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D
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D

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I

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S
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S

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K

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A

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L

A

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P

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x
S

F

L

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I

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S

D

D

K

K

A

A

-

K

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A

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A

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x
R

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K

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T

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K

-

P

-

F

-

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-

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-

Y

-

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-
x
I

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Y

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W

D

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W
x
C

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P

-

N

-

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-

N

-

G

-

F

M

F

Y

P

H

Y

H

T

T

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A

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N

I

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Y

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S

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L

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R

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E

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A

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x
I

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A

E

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A

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T

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x
V

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P

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A

G

G

H

Y

D

D

A

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D

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Q

D

F

I

R

V

A

S

L

Y

V

V

L
x
I

E

D

K

H

Y

F

N

D

M

I

S

E

L

I

G

M

A

G

G

G

L

L

T

G

N

P

L

S

A

T

G

G

K

K

V

V

F

L

R

R

I

I

G

G

H

L

L

L

G

G

E

-

C

C

N

N

R36 (= R21) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
G41 (≠ L26) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; associated in cis with L-11 and M-340; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
G47 (≠ D32) to R: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
G82 (= G66) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
W108 (≠ F92) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
A112 (≠ W96) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
L150 (≠ A137) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
F152 (≠ M139) to I: in HP1; associated in cis with L-11 and M-340; results in protein destabilization; no loss of dimerization; decreased alanine--glyoxylate aminotransferase activity; loss of alanine--glyoxylate aminotransferase activity when associated with L-11 and M-340; mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908524
G156 (≠ N143) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
S158 (≠ T145) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
G161 (= G148) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
L166 (≠ I153) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
G170 (≠ R157) to R: in HP1; associated in cis with L-11 and M-340; decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; results in mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908529
C173 (≠ I160) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
D183 (= D172) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
S187 (= S176) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
I202 (≠ V191) to N: in HP1; uncertain significance; dbSNP:rs536352238
S205 (= S194) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
K209 (= K198) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
S218 (≠ G207) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
R233 (≠ S219) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
I244 (vs. gap) to T: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity in vitro; no loss of dimerization; partial mitochondrial mistargeting; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; dbSNP:rs121908525
C253 (≠ W231) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
I279 (≠ L262) to T: in dbSNP:rs140992177
A280 (≠ L263) to V: in dbSNP:rs73106685
V326 (≠ M309) to I: in dbSNP:rs115057148
I340 (≠ L323) to M: in allele minor; associated in cis with L-11; no effect on alanine--glyoxylate aminotransferase activity in vitro; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with L-11; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and I-152; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and R-170; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and T-244; results in mitochondrial mistargeting when associated with L-11 and R-170; dbSNP:rs4426527

Sites not aligning to the query:

9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
11 P → L: in allele minor; associated in cis with M-340; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with I-152 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with R-170 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with T-244 and M-340; causes mitochondrial mistargeting when associated with R-170 and M-340; dbSNP:rs34116584

1h0cA The crystal structure of human alanine:glyoxylate aminotransferase (see paper)
30% identity, 85% coverage: 13:351/398 of query aligns to 25:362/385 of 1h0cA

query
sites
1h0cA

P
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G
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A

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S

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V

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K
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K

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L

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P

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G

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G

S

F

L

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A

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S

D

D

K

K

A

A

-

K

L

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A

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A

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S

R

K

K

T

T

N

K

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P

-

F

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S

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F

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Y

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L

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-

I

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M

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P

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A

S

N

Y

F

W

W

D

G

W

C

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D

M

Q

L

P

K

R

P

-

N

-

A

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N

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G

-

F

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F

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Y

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T

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A

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L
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R

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L

G

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N

N

binding (aminooxy)acetic acid: P25 (= P13), G26 (= G14), L346 (= L334), R355 (= R343)
binding pyridoxal-5'-phosphate: S78 (= S65), G79 (= G66), H80 (≠ T67), W105 (= W96), S153 (≠ T145), D178 (= D172), V180 (≠ I174), K204 (= K198)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 385

Q0IG34 3-hydroxykynurenine transaminase; 3-hydroxykynurenine transaminase and alanine--glyoxylate aminotransferase; Ae-HKT/AGT; Alanine--glyoxylate aminotransferase; EC 2.6.1.63; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
29% identity, 87% coverage: 13:357/398 of query aligns to 24:370/400 of Q0IG34

query
sites
Q0IG34

P

P

G

G

P

P

S

S

P

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P

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K

R

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I

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A

A

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L

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R

-

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V

C

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I

Y

T

P

G

S
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S

S
x
A

G
x
H

T

T

G

G

A

M

W

-

E

E

A

A

A

L

I

L

V

C

N

N

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L

L

L

S

E

P

E

G

G

D

D

K

I

V

V

L

L

M

I

V

A

E

N

T

N

G

G

H

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F

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A

A

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L

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W

A

R

I

K

N

M

M

A

A

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Y

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A

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D

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V

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A

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A

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A

K

K

A

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A

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K

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M

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H

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x
S

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K

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-

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D

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P

D

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A

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K

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W

G

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V

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D

D

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S

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C

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S

S

Q
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Q

K
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K

G

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L

L

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G

L

A

P

P

G

G

L

I

G

T

F

P

T

I

A

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I

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D

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K

K

A

A

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L

A

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A

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K

Y

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F

D

Y

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W

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L

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L

P

G

N

N

A

Y

N

W

G

G

F

C

F

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P

D

-

E

-

Q

-

K

-

R

-
x
Y

-

H

Y

H

T
|
T

P

V

A

P

T

S

N

N

L

L

L

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Y

F

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A

A

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I

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A

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E

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G

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L

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A

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M

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G

L

L

E

E

I

I

L

F

C

V

L

K

E

D

P

P

A

E

E

Y

Y

R

S

L

P

P

V

T

L

V

T

T

A

C

I

I

L

M

M

I

P

P

Q

K

G

G

H

V

D

N

A

W

D

W

Q

K

F

V

R

S

A

E

L

Y

V

A

L

M

E

N

K

N

Y

F

N

S

M

L

S

E

L

I

G

Q

A

G

G

G

L

F

T

G

N

P

L

T

A

M

G

G

K

I

V

A

F

W

R

R

I

A

G

G

H

I

L

M

G

G

E

E

C

S

N

S

E

T

L

L

T

Q

L

R

I

V

G

N

SAH 77:79 (≠ SSG 64:66) binding in other chain
S154 (≠ T145) binding in other chain
Q204 (= Q197) binding in other chain
K205 (= K198) modified: N6-(pyridoxal phosphate)lysine
Y256 (vs. gap) binding pyridoxal 5'-phosphate
T259 (= T246) binding pyridoxal 5'-phosphate

6mfbD Crystal structure of 3-hydroxykynurenine transaminase from aedes aegypti
29% identity, 87% coverage: 13:357/398 of query aligns to 24:370/386 of 6mfbD

query
sites
6mfbD

P

P

G

G

P

P

S

S

P

N

V

C

P

S

E

K

R

R

I

V

L

L

R

A

A

A

L

L

S

N

M

N

Q

T

V

C

I

L

D

S

H

N

R

F

G

H

P

D

E

E

F

L

G

F

A

Q

L

V

G

I

R

D

E

E

V

V

L

K

A

D

G

G

C

L

Q

R

T

Y

I

I

F

F

K

Q

T

T

S

E

G

N

-

R

-

T

P

T

V

M

V

C

I

I

Y

T

P

G

S
|
S

S
x
A

G
x
H

T

T

G

G

A

M

W

-

E

E

A

A

A

L

I

L

V

C

N

N

T

L

L

L

S

E

P

E

G

G

D

D

K

I

V

V

L

L

M

I

V

A

E

N

T

N

G

G

H

I

F
x
W

A

A

T

E

L

R

W

A

R

I

K

N

M

M

A

A

G

T

R

R

W

Y

G

G

L

A

D

D

V

V

D

R

F

V

L

L

A

E

G

G

D

P

W

A

R

D

H

K

G

P

A

F

D

S

P

M

A

T

A

D

I

F

E

K

A

K

K

A

L

I

A

E

E

Q

D

H

K

R

P

P

K

K

S

C

I

L

K

-

A

-

V

-

M

F

V

V

H

H

N

G

E

D

T
x
S

S

S

T

S

G

G

V

L

I

L

S

Q

R

P

I

L

A

E

E

G

I

L

R

G

K

K

A

I

I

C

D

H

K

-

A

-

N

D

H

Y

P

D

A

C

L

L

M

L

V

V

D
|
D

T

A

I
x
V

S

A

S

S

L

L

G

C

S

G

A

V

D

P

Y

F

R

Y

H

M

E

D

E

K

W

W

G

E

V

I

D

D

V

G

T

V

V

Y

S

T

C

G

S

S

Q
|
Q

K
|
K

G

V

L

L

M

G

L

A

P

P

G

G

L

I

G

T

F

P

T

I

A

S

I

I

S

S

D

P

K

K

A

A

L

L

A

E

A

V

S

I

K

R

T

S

N

R

K

K

M

T

P

P

R

S

S

K

Y

V

W

F

D

Y

W

W

E

D

E

L

M

L

L

I

K

L

P

G

N

N

A

Y

N

W

G

G

F

C

F

Y

P

D

-

E

-

Q

-

K

-

R

-
x
Y

-

H

Y

H

T
|
T

P

V

A

P

T

S

N

N

L

L

L

I

Y

F

G

A

L

L

R

R

E

E

A

A

I

I

K

A

I

Q

L

I

L

A

E

E

G

G

L

L

D

E

T

P

V

V

F

I

A

R

R

R

H

R

Q

Q

H

E

L

C

A

A

A

E

A

Q

T

M

R

Y

A

R

A

G

V

L

R

Q

A

A

W

M

G

G

L

L

E

E

I

I

L

F

C

V

L

K

E

D

P

P

A

E

E

Y

Y

R

S

L

P

P

V

T

L

V

T

T

A

C

I

I

L

M

M

I

P

P

Q

K

G

G

H

V

D

N

A

W

D

W

Q

K

F

V

R

S

A

E

L

Y

V

A

L

M

E

N

K

N

Y

F

N

S

M

L

S

E

L

I

G

Q

A

G

G

G

L

F

T

G

N

P

L

T

A

M

G

G

K

I

V

A

F

W

R

R

I

A

G

G

H

I

L

M

G

G

E

E

C

S

N

S

E

T

L

L

T

Q

L

R

I

V

G

N

binding pyridoxal-5'-phosphate: S77 (= S64), A78 (≠ S65), H79 (≠ G66), W104 (≠ F92), S154 (≠ T145), D179 (= D172), V181 (≠ I174), Q204 (= Q197), K205 (= K198), Y256 (vs. gap), T259 (= T246)

3kgxA Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.80 a resolution
30% identity, 84% coverage: 13:348/398 of query aligns to 24:358/383 of 3kgxA

query
sites
3kgxA

P

P

G

G

P

P

S

S

P

N

V

L

P

A

E

P

R

R

I

V

L

L

R

A

A

A

L

G

S

S

M

L

Q

R

V

M

I

I

D

G

H

H

R

M

G

Q

P

K

E

E

F

M

G

L

A

Q

L

I

G

M

R

E

E

E

V

I

L

K

A

Q

G

G

C

I

Q

Q

T

Y

I

V

F

F

K

Q

T

T

S

R

G

N

P

P

V

L

V

T

I

L

Y

V

P

V

S

S

-

G

S

S

G

G

T

H

G

C

A

A

W

M

E

E

A

T

A

A

I

L

V

F

N

N

T

L

L

L

S

E

P

P

G

G

D

D

K

S

V

F

L

L

M

T

V

G

E

T

T

N

G

G

H

I

F

W

A

G

T

M

L

R

W

A

R

A

K

E

M

I

A

A

G

D

R

R

W

I

G

G

L

A

D

R

V

V

D

H

F

Q

L

M

A

I

G

K

D

K

W

P

R

G

H

E

G

H

A

Y

D

T

P

L

A

Q

A

E

I

V

E

E

A

E

K

G

L

L

A

A

E

Q

D

H

K

K

P

P

K

V

S

-

I

-

K

-

A

L

V

L

M

F

V

L

V

V

H

H

N

G

E

E

T

S

S

S

T

T

G

G

V

V

I

V

S

Q

R

P

I

L

A

D

E

G

I

F

R

G

K

E

A

L

I

C

D

H

K

R

A

-

N

-

H

Y

P

Q

A

C

L

L

M

L

V

V

D

D

T

S

I

V

S

A

S

S

L

L

G

G

S

G

A

V

D

P

Y

I

R

Y

H

M

E

D

E

Q

W

Q

G

G

V

I

D

D

V

I

T

M

V

Y

S

S

C

S

S

S

Q

Q

K

K

G

V

L

L

M

N

L

A

P

P

G

G

L

I

G

S

F

L

T

I

A

S

I

F

S

N

D

D

K

K

A

A

L

K

-

Y

-

K

A

V

A

Y

S

S

K

R

T

K

N

T

K

K

M

P

P

V

R

S

S

F

Y

Y

W

T

D

D

W

I

E

T

E

Y

M

L

L

A

K

K

P

L

-

W

N

G

A

C

N

E

G

G

F

V

F

I

P

H

Y

H

T

T

P

T

A

P

T

V

N

T

L

S

L

L

Y

Y

G

C

L

L

R

R

E

E

A

S

I

L

K

A

I

L

L

I

L

A

E

E

E

Q

G

G

L

L

D

E

T

N

V

C

F

W

A

R

R

R

H

H

Q

R

H

E

L

A

A

T

A

A

A
x
H

T

L

R

H

A

K

A
x
H

V

L

R

Q

A

E

W

M

G

G

L

L

E

K

I

F

L

F

C

V

L

K

E

D

P

P

A

E

E

I

Y

R

S

L

P

P

V

T

L

I

T

T

A

T

I

V

L

T

M

V

P

P

Q

A

G

G

H

Y

D

N

A

W

D

R

Q

D

F

I

R

V

A

S

L

Y

V

V

L

L

E

D

K

H

Y

F

N

S

M

I

S

E

L

I

G

S

A

G

G

G

L

L

T

G

N

P

L

T

A

E

G

E

K

R

V

V

F

L

R

R

I

I

G

G

H

L

L

L

G

G

binding magnesium ion: H290 (≠ A280), H294 (≠ A284)

Sites not aligning to the query:

binding magnesium ion: 367

3kgwB Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.65 a resolution
30% identity, 84% coverage: 13:348/398 of query aligns to 28:363/388 of 3kgwB

query
sites
3kgwB

P

P

G

G

P

P

S

S

P

N

V

L

P

A

E

P

R

R

I

V

L

L

R

A

A

A

L

G

S

S

M

L

Q

R

V

M

I

I

D

G

H

H

R

M

G

Q

P

K

E

E

F

M

G

L

A

Q

L

I

G

M

R

E

E

E

V

I

L

K

A

Q

G

G

C

I

Q

Q

T

Y

I

V

F

F

K

Q

T

T

S

R

G

N

P

P

V

L

V

T

I

L

Y

V

P

V

S

S

-

G

S
|
S

G
|
G

T
x
H

G

C

A

A

W

M

E

E

A

T

A

A

I

L

V

F

N

N

T

L

L

L

S

E

P

P

G

G

D

D

K

S

V

F

L

L

M

T

V

G

E

T

T

N

G

G

H

I

F
x
W

A

G

T

M

L

R

W

A

R

A

K

E

M

I

A

A

G

D

R

R

W

I

G

G

L

A

D

R

V

V

D

H

F

Q

L

M

A

I

G

K

D

K

W

P

R

G

H

E

G

H

A

Y

D

T

P

L

A

Q

A

E

I

V

E

E

A

E

K

G

L

L

A

A

E

Q

D

H

K

K

P

P

K

V

S

-

I

-

K

-

A

L

V

L

M

F

V

L

V

V

H

H

N

G

E

E

T
x
S

S

S

T

T

G

G

V

V

I

V

S

Q

R

P

I

L

A

D

E

G

I

F

R

G

K

E

A

L

I

C

D

H

K

R

A

-

N

-

H

Y

P

Q

A

C

L

L

M

L

V

V

D
|
D

T

S

I
x
V

S

A

S

S

L

L

G

G

S

G

A

V

D

P

Y

I

R

Y

H

M

E

D

E

Q

W

Q

G

G

V

I

D

D

V

I

T

M

V

Y

S

S

C

S

S

S

Q

Q

K
|
K

G

V

L

L

M

N

L

A

P

P

G

G

L

I

G

S

F

L

T

I

A

S

I

F

S

N

D

D

K

K

A

A

L

K

-

Y

-

K

A

V

A

Y

S

S

K

R

T

K

N

T

K

K

M

P

P

V

R

S

S

F

Y

Y

W

T

D

D

W

I

E

T

E

Y

M

L

L

A

K

K

-

L

-

W

-

G

P

C

N

E

A

T

N

R

G

V

F

I

F

H

P

H

Y

T

T

T

P

P

A

V

T

T

N

S

L

L

L

-

Y

Y

G

C

L

L

R

R

E

E

A

S

I

L

K

A

I

L

L

I

L

A

E

E

E

Q

G

G

L

L

D

E

T

N

V

C

F

W

A

R

R

R

H

H

Q

R

H

E

L

A

A

T

A

A

A

H

T

L

R

H

A

K

A

H

V

L

R

Q

A

E

W

M

G

G

L

L

E

K

I

F

L

F

C

V

L

K

E

D

P

P

A

E

E

I

Y

R

S

L

P

P

V

T

L

I

T

T

A

T

I

V

L

T

M

V

P

P

Q

A

G

G

H

Y

D

N

A

W

D

R

Q

D

F

I

R

V

A

S

L

Y

V

V

L

L

E

D

K

H

Y

F

N

S

M

I

S

E

L

I

G

S

A

G

G

G

L

L

T

G

N

P

L

T

A

E

G

E

K

R

V

V

F

L

R

R

I

I

G

G

H

L

L

L

G

G

binding pyridoxal-5'-phosphate: S81 (= S65), G82 (= G66), H83 (≠ T67), W108 (≠ F92), S158 (≠ T145), D183 (= D172), V185 (≠ I174), K209 (= K198)

Query Sequence

>WP_012386429.1 NCBI__GCF_000019845.1:WP_012386429.1
MTTQQGRHFLQIPGPSPVPERILRALSMQVIDHRGPEFGALGREVLAGCQTIFKTSGPVV
IYPSSGTGAWEAAIVNTLSPGDKVLMVETGHFATLWRKMAGRWGLDVDFLAGDWRHGADP
AAIEAKLAEDKPKSIKAVMVVHNETSTGVISRIAEIRKAIDKANHPALLMVDTISSLGSA
DYRHEEWGVDVTVSCSQKGLMLPPGLGFTAISDKALAASKTNKMPRSYWDWEEMLKPNAN
GFFPYTPATNLLYGLREAIKILLEEGLDTVFARHQHLAAATRAAVRAWGLEILCLEPAEY
SPVLTAILMPQGHDADQFRALVLEKYNMSLGAGLTNLAGKVFRIGHLGECNELTLIGTLG
GVEMGLAAAGVPHKAGGVLAAMQALGDLNTAVSQLAAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory