SitesBLAST
Comparing WP_012386450.1 NCBI__GCF_000019845.1:WP_012386450.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
44% identity, 87% coverage: 9:266/295 of query aligns to 5:267/306 of 4whxA
2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
42% identity, 87% coverage: 9:266/295 of query aligns to 3:265/305 of 2ej0B
- active site: F35 (= F41), G37 (= G43), K158 (= K159), E192 (= E193), L215 (= L216)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R60), Y163 (= Y164), E192 (= E193), G195 (= G196), E196 (≠ A197), L215 (= L216), G217 (= G218), I218 (= I219), T219 (= T220), G254 (= G255), T255 (= T256)
2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
42% identity, 87% coverage: 9:266/295 of query aligns to 3:257/297 of 2ej3A
- active site: F35 (= F41), G37 (= G43), K150 (= K159), E184 (= E193), L207 (= L216)
- binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G196), G246 (= G255), T247 (= T256), A248 (≠ G257)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)
2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
42% identity, 87% coverage: 9:266/295 of query aligns to 3:257/297 of 2eiyA
- active site: F35 (= F41), G37 (= G43), K150 (= K159), E184 (= E193), L207 (= L216)
- binding 4-methyl valeric acid: F35 (= F41), Y94 (= Y96), T247 (= T256), A248 (≠ G257)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), E188 (≠ A197), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)
1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
42% identity, 87% coverage: 9:266/295 of query aligns to 3:257/297 of 1wrvA
- active site: F35 (= F41), G37 (= G43), K150 (= K159), E184 (= E193), L207 (= L216)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), T247 (= T256)
2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
41% identity, 87% coverage: 9:266/295 of query aligns to 3:254/294 of 2ej2A
- active site: F35 (= F41), G37 (= G43), K147 (= K159), E181 (= E193), L204 (= L216)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R60), Y94 (= Y96), Y152 (= Y164), E181 (= E193), G184 (= G196), E185 (≠ A197), L204 (= L216), G206 (= G218), I207 (= I219), T208 (= T220), T244 (= T256), A245 (≠ G257)
1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
39% identity, 86% coverage: 12:266/295 of query aligns to 4:264/304 of 1iyeA
- active site: F33 (= F41), G35 (= G43), K156 (= K159), A157 (= A160), E190 (= E193), L214 (= L216)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R60), Y92 (= Y96), Y126 (≠ M129), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (≠ A197), N195 (= N198), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (≠ G257)
1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
39% identity, 86% coverage: 12:266/295 of query aligns to 4:264/304 of 1iydA
- active site: F33 (= F41), G35 (= G43), K156 (= K159), A157 (= A160), E190 (= E193), L214 (= L216)
- binding glutaric acid: Y92 (= Y96), Y126 (≠ M129), A255 (≠ G257)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (≠ A197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), T254 (= T256)
1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
39% identity, 86% coverage: 12:266/295 of query aligns to 4:264/304 of 1i1mA
- active site: K156 (= K159)
- binding 4-methyl valeric acid: Y92 (= Y96), K156 (= K159), T254 (= T256), A255 (≠ G257)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (≠ A197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256)
1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
39% identity, 86% coverage: 12:266/295 of query aligns to 4:264/304 of 1i1lA
- active site: K156 (= K159)
- binding 2-methylleucine: Y92 (= Y96), K156 (= K159), T254 (= T256), A255 (≠ G257)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), G216 (= G218), I217 (= I219), T218 (= T220), T254 (= T256)
6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
37% identity, 92% coverage: 13:284/295 of query aligns to 9:282/301 of 6thqB
- active site: F37 (= F41), K156 (= K159), E190 (= E193), L214 (= L216)
- binding pyridoxal-5'-phosphate: R60 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), N195 (= N198), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), T254 (= T256)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R60), Y97 (= Y96), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (≠ A197), N195 (= N198), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (≠ G257)
5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
33% identity, 93% coverage: 12:285/295 of query aligns to 3:278/290 of 5mr0D
- active site: F32 (= F41), G34 (= G43), K150 (= K159), E183 (= E193), L206 (= L216)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R60), G100 (≠ A109), L101 (≠ V110), K150 (= K159), Y154 (= Y164), E183 (= E193), G186 (= G196), D187 (≠ A197), L206 (= L216), I209 (= I219), T210 (= T220), G245 (= G255), T246 (= T256)
5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
33% identity, 92% coverage: 13:284/295 of query aligns to 5:278/290 of 5e25A
- active site: F33 (= F41), G35 (= G43), K151 (= K159), E184 (= E193), L207 (= L216)
- binding 2-oxoglutaric acid: Y88 (= Y96), K151 (= K159), T247 (= T256), A248 (≠ G257)
- binding pyridoxal-5'-phosphate: R52 (= R60), K151 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), D188 (≠ A197), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)
6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
32% identity, 93% coverage: 12:284/295 of query aligns to 5:286/307 of 6q8eA
- active site: F34 (= F41), K156 (= K159), E190 (= E193), L214 (= L216)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), S194 (≠ A197), C195 (≠ N198), L214 (= L216), S216 (≠ G218), I217 (= I219), T218 (= T220), G254 (= G255), T255 (= T256)
7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
31% identity, 93% coverage: 12:284/295 of query aligns to 5:286/309 of 7neaA
- active site: F34 (= F41), K156 (= K159), E190 (= E193), L214 (= L216)
- binding pyridoxal-5'-phosphate: R59 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), S194 (≠ A197), L214 (= L216), S216 (≠ G218), I217 (= I219), T218 (= T220), T255 (= T256)
7p3tB Transaminase of gamma-proteobacterium (see paper)
30% identity, 92% coverage: 13:284/295 of query aligns to 6:279/299 of 7p3tB
- binding pyridoxal-5'-phosphate: R53 (= R60), K153 (≠ A158), R157 (≠ G162), E186 (= E193), S187 (≠ C194), A188 (≠ T195), A189 (≠ G196), S190 (≠ A197), G210 (= G218), I211 (= I219), T212 (= T220), T248 (= T256)
6fteB Crystal structure of an (r)-selective amine transaminase from exophiala xenobiotica
25% identity, 90% coverage: 2:266/295 of query aligns to 21:284/321 of 6fteB
- active site: Y58 (≠ F41), K179 (= K159), E212 (= E193), L234 (= L216)
- binding pyridoxal-5'-phosphate: R77 (= R60), K179 (= K159), E212 (= E193), F216 (≠ A197), N217 (= N198), L234 (= L216), G236 (= G218), V237 (≠ I219), T238 (= T220), T274 (= T256)
3lqsA Complex structure of d-amino acid aminotransferase and 4-amino-4,5- dihydro-thiophenecarboxylic acid (adta) (see paper)
27% identity, 87% coverage: 11:266/295 of query aligns to 1:251/280 of 3lqsA
- active site: Y31 (≠ F41), V33 (≠ G43), K145 (= K159), E177 (= E193), L201 (= L216)
- binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]thiophene-2-carboxylic acid: V33 (≠ G43), R50 (= R60), E177 (= E193), S180 (≠ G196), S181 (≠ A197), N182 (= N198), L201 (= L216), G203 (= G218), I204 (= I219), T205 (= T220), S240 (≠ G255), T241 (= T256), T242 (≠ G257)
P19938 D-alanine aminotransferase; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase; EC 2.6.1.21 from Bacillus sp. (strain YM-1) (see 5 papers)
27% identity, 87% coverage: 11:266/295 of query aligns to 2:252/283 of P19938
- Y32 (≠ F41) binding
- R51 (= R60) binding
- R99 (≠ M108) binding
- H101 (≠ V110) binding
- K146 (= K159) active site, Proton acceptor; modified: N6-(pyridoxal phosphate)lysine
- E178 (= E193) binding ; mutation to K: Loss of transaminase activity and small gain in racemase activity.
- L202 (= L216) mutation to A: Inactivates enzyme.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3daaA Crystallographic structure of d-amino acid aminotransferase inactivated by pyridoxyl-d-alanine (see paper)
27% identity, 87% coverage: 11:266/295 of query aligns to 1:251/277 of 3daaA
- active site: Y31 (≠ F41), V33 (≠ G43), K145 (= K159), E177 (= E193), L201 (= L216)
- binding n-(5'-phosphopyridoxyl)-d-alanine: Y31 (≠ F41), R50 (= R60), K145 (= K159), E177 (= E193), S180 (≠ G196), S181 (≠ A197), L201 (= L216), G203 (= G218), I204 (= I219), T205 (= T220), S240 (≠ G255), T241 (= T256)
Query Sequence
>WP_012386450.1 NCBI__GCF_000019845.1:WP_012386450.1
MSALPFDQRDGYIWMNGRIVAWADAKLHVLSHGLHYASCVFEGERAYEGRIFKSREHSER
FRASAQVLDFELPYSVAELDAAKQLVVDKNNFQNCYVRPVAWRGSEMMAVAAQNSTINVA
IAAWDWPSMFDMETKMKGIRLDIADYRRPDPQTAPCHAKAAGLYMICTISKHRAERRGYA
DAMMLDWQGRVAECTGANLFFTQKGALHTPIADCFLNGITRQTVIELAKRRGLEVVERRI
MPDELSGFDECFVCGTGAEVTPVSEIGPYRFTPGVISRALIEDYSLEVRPRQQAA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory