SitesBLAST
Comparing WP_012399852.1 NCBI__GCF_000020045.1:WP_012399852.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q6BF17 D-galactonate dehydratase; GalD; EC 4.2.1.6 from Escherichia coli (strain K12)
63% identity, 100% coverage: 1:382/382 of query aligns to 1:382/382 of Q6BF17
- H185 (= H185) mutation H->N,Q: Loss of activity.
- H285 (= H285) mutation to N: Loss of activity.
- E310 (= E310) mutation to Q: Loss of activity.
3rraB Crystal structure of enolase prk14017 (target efi-500653) from ralstonia pickettii 12j with magnesium bound
61% identity, 100% coverage: 1:382/382 of query aligns to 2:375/379 of 3rraB
- active site: I35 (≠ V34), R38 (= R37), Y118 (= Y117), K145 (= K144), N147 (= N146), E151 (= E150), D184 (= D183), H186 (= H185), E210 (= E209), G235 (= G234), E236 (= E235), R237 (= R236), Q257 (= Q256), D259 (= D258), H286 (= H285), P288 (= P287), E311 (= E310)
- binding magnesium ion: D184 (= D183), E210 (= E209), E236 (= E235)
3rgtA Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with d-arabinohydroxamate
34% identity, 100% coverage: 1:382/382 of query aligns to 1:383/383 of 3rgtA
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), D191 (= D183), H193 (= H185), E217 (= E209), G242 (= G234), E243 (= E235), R264 (≠ Q256), P266 (≠ D258), H293 (= H285), G294 (≠ C286), E320 (= E310), W383 (= W382)
- binding cobalt (ii) ion: D191 (= D183), E217 (= E209), E243 (= E235)
- binding (2S,3R,4R)-2,3,4,5-tetrahydroxy-N-oxo-pentanamide: N37 (≠ E35), D191 (= D183), H193 (= H185), E243 (= E235), H293 (= H285), P295 (= P287), D297 (vs. gap), E320 (= E310), W383 (= W382)
3p93C Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg,d-mannonate and 2-keto-3-deoxy-d- gluconate
34% identity, 100% coverage: 1:382/382 of query aligns to 1:384/384 of 3p93C
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), D192 (= D183), H194 (= H185), E218 (= E209), G243 (= G234), E244 (= E235), R265 (≠ Q256), P267 (≠ D258), H294 (= H285), G295 (≠ C286), E321 (= E310), W384 (= W382)
- binding 2-keto-3-deoxygluconate: N37 (≠ E35), D192 (= D183), H194 (= H185), E244 (= E235), H294 (= H285), P296 (= P287), D298 (vs. gap), E321 (= E310), W384 (= W382)
- binding magnesium ion: D192 (= D183), E218 (= E209), E244 (= E235)
3p93A Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg,d-mannonate and 2-keto-3-deoxy-d- gluconate
34% identity, 100% coverage: 1:382/382 of query aligns to 1:384/384 of 3p93A
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), D192 (= D183), H194 (= H185), E218 (= E209), G243 (= G234), E244 (= E235), R265 (≠ Q256), P267 (≠ D258), H294 (= H285), G295 (≠ C286), E321 (= E310), W384 (= W382)
- binding d-mannonic acid: N37 (≠ E35), D192 (= D183), H194 (= H185), E244 (= E235), H294 (= H285), P296 (= P287), D298 (vs. gap), E321 (= E310)
- binding magnesium ion: D192 (= D183), E218 (= E209), E244 (= E235)
4e4fB Crystal structure of enolase pc1_0802 (target efi-502240) from pectobacterium carotovorum subsp. Carotovorum pc1
34% identity, 100% coverage: 1:382/382 of query aligns to 2:381/381 of 4e4fB
- active site: L37 (≠ V34), R40 (= R37), R148 (≠ K144), Q150 (≠ N146), D189 (= D183), H191 (= H185), E215 (= E209), G240 (= G234), E241 (= E235), V242 (≠ R236), R262 (≠ Q256), T264 (≠ D258), H291 (= H285), P293 (= P287), E318 (= E310), W381 (= W382)
- binding magnesium ion: D189 (= D183), E215 (= E209), E241 (= E235)
3v3wA Crystal structure of an enolase from the soil bacterium cellvibrio japonicus (target efi-502161) with bound mg and glycerol
31% identity, 100% coverage: 1:382/382 of query aligns to 1:397/397 of 3v3wA
- active site: L36 (≠ V34), R39 (= R37), H122 (≠ W119), K144 (= K141), R147 (≠ K144), Q149 (≠ N146), Y159 (vs. gap), E179 (≠ F157), D205 (= D183), H207 (= H185), E231 (= E209), G256 (= G234), E257 (= E235), V258 (≠ R236), R278 (≠ Q256), T280 (≠ D258), F306 (≠ L284), H307 (= H285), G308 (vs. gap), A309 (vs. gap), E334 (= E310), W397 (= W382)
- binding magnesium ion: D205 (= D183), E231 (= E209), E257 (= E235)
B3PDB1 D-galactonate dehydratase family member RspA; D-mannonate dehydratase; Starvation sensing protein RspA homolog; EC 4.2.1.-; EC 4.2.1.8 from Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) (see paper)
31% identity, 100% coverage: 1:382/382 of query aligns to 1:402/402 of B3PDB1
- D210 (= D183) binding Mg(2+)
- E236 (= E209) binding Mg(2+)
- E262 (= E235) binding Mg(2+)
C6CBG9 D-galactonate dehydratase family member Dd703_0947; D-gluconate dehydratase; D-mannonate dehydratase; EC 4.2.1.-; EC 4.2.1.39; EC 4.2.1.8 from Musicola paradisiaca (strain Ech703) (Dickeya paradisiaca) (Dickeya dadantii) (see paper)
34% identity, 93% coverage: 1:354/382 of query aligns to 4:389/417 of C6CBG9
- D223 (vs. gap) binding Mg(2+)
- E249 (= E209) binding Mg(2+)
- E275 (= E235) binding Mg(2+)
4ihcB Crystal structure of probable mannonate dehydratase dd703_0947 (target efi-502222) from dickeya dadantii ech703
35% identity, 93% coverage: 1:354/382 of query aligns to 2:367/395 of 4ihcB
- active site: F39 (≠ V34), R42 (= R37), R150 (≠ K144), Q152 (vs. gap), D201 (vs. gap), H203 (= H185), E227 (= E209), G252 (= G234), E253 (= E235), L254 (≠ R236), R274 (≠ Q256), H276 (≠ D258), H303 (= H285), P305 (= P287), E330 (= E310)
- binding magnesium ion: D201 (vs. gap), E227 (= E209), E253 (= E235)
Sites not aligning to the query:
Q1QT89 D-galactonate dehydratase family member ManD; D-gluconate dehydratase; D-mannonate dehydratase; EC 4.2.1.-; EC 4.2.1.39; EC 4.2.1.8 from Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11) (see paper)
33% identity, 100% coverage: 1:382/382 of query aligns to 1:403/403 of Q1QT89
- D211 (= D183) binding Mg(2+)
- E237 (= E209) binding Mg(2+)
- E263 (= E235) binding Mg(2+)
- H313 (= H285) mutation to N: Abolishes activity with D-gluconate and D-mannonate.; mutation to Q: Abolishes activity with D-gluconate. No effect on activity with D-mannonate.
- P315 (= P287) mutation to A: Strongly increases activity with D-mannonate. Slightly decreases activity with D-gluconate.
D4GJ14 D-galactonate dehydratase family member RspA; D-gluconate dehydratase; Starvation sensing protein RspA homolog; EC 4.2.1.-; EC 4.2.1.39 from Pantoea ananatis (strain LMG 20103) (see paper)
34% identity, 99% coverage: 3:379/382 of query aligns to 6:414/417 of D4GJ14
- D223 (= D183) binding Mg(2+)
- E249 (= E209) binding Mg(2+)
- E275 (= E235) binding Mg(2+)
3t6cA Crystal structure of an enolase from pantoea ananatis (efi target efi- 501676) with bound d-gluconate and mg
34% identity, 99% coverage: 3:379/382 of query aligns to 4:412/415 of 3t6cA
- active site: F39 (≠ V34), R42 (= R37), R150 (≠ K144), Q152 (≠ N146), D162 (≠ E149), D221 (= D183), H223 (= H185), E247 (= E209), G272 (= G234), E273 (= E235), L274 (≠ R236), R294 (≠ Q256), H296 (≠ D258), H323 (= H285), P325 (= P287), E350 (= E310)
- binding D-gluconic acid: Y156 (vs. gap), L166 (≠ I153), D221 (= D183), H223 (= H185), E273 (= E235), H323 (= H285), P325 (= P287), D327 (vs. gap), E350 (= E310)
- binding magnesium ion: D221 (= D183), E247 (= E209), E273 (= E235)
Sites not aligning to the query:
4kwsA Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and glycerol
33% identity, 100% coverage: 1:382/382 of query aligns to 1:397/397 of 4kwsA
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), Y159 (vs. gap), D205 (= D183), H207 (= H185), E231 (= E209), G256 (= G234), E257 (= E235), R278 (≠ Q256), P280 (≠ D258), H307 (= H285), G308 (≠ C286), E334 (= E310), W397 (= W382)
- binding magnesium ion: D205 (= D183), E231 (= E209), E257 (= E235)
3qkeA Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and d-gluconate
33% identity, 100% coverage: 1:382/382 of query aligns to 1:397/397 of 3qkeA
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), Y159 (vs. gap), D205 (= D183), H207 (= H185), E231 (= E209), G256 (= G234), E257 (= E235), R278 (≠ Q256), P280 (≠ D258), H307 (= H285), G308 (≠ C286), E334 (= E310), W397 (= W382)
- binding D-gluconic acid: N37 (≠ E35), Y159 (vs. gap), D205 (= D183), H207 (= H185), E257 (= E235), H307 (= H285), P309 (= P287), D311 (vs. gap), E334 (= E310), W397 (= W382)
- binding magnesium ion: D205 (= D183), E231 (= E209), E257 (= E235)
4k1wA Crystal structure of the a314p mutant of mannonate dehydratase from novosphingobium aromaticivorans complexed with mg and d-mannonate
35% identity, 100% coverage: 1:382/382 of query aligns to 11:395/395 of 4k1wA
- active site: G131 (≠ S118), R157 (≠ K144), Q159 (≠ N146), D203 (= D183), H205 (= H185), E229 (= E209), G254 (= G234), E255 (= E235), R276 (≠ Q256), T278 (≠ D258), H305 (= H285), E332 (= E310), W395 (= W382)
- binding d-mannonic acid: N47 (≠ E35), D203 (= D183), H205 (= H185), E255 (= E235), H305 (= H285), P307 (= P287), D309 (vs. gap), E332 (= E310), L382 (vs. gap), W395 (= W382)
- binding magnesium ion: D203 (= D183), E229 (= E209), E255 (= E235)
- binding 1,3-propandiol: W174 (vs. gap), H202 (≠ V182), Y208 (≠ V188)
2qjnA Crystal structure of d-mannonate dehydratase from novosphingobium aromaticivorans complexed with mg and 2-keto-3-deoxy-d-gluconate (see paper)
35% identity, 100% coverage: 1:382/382 of query aligns to 1:385/385 of 2qjnA
- active site: G121 (≠ S118), R147 (≠ K144), Q149 (≠ N146), D193 (= D183), H195 (= H185), E219 (= E209), G244 (= G234), E245 (= E235), R266 (≠ Q256), T268 (≠ D258), H295 (= H285), E322 (= E310), W385 (= W382)
- binding 2-keto-3-deoxygluconate: N37 (≠ E35), D193 (= D183), H195 (= H185), E245 (= E235), H295 (= H285), A297 (vs. gap), D299 (≠ P287), E322 (= E310), L372 (≠ N369), W385 (= W382)
- binding magnesium ion: D193 (= D183), E219 (= E209), E245 (= E235)
3twbC Crystal structure of gluconate dehydratase (target efi-501679) from salmonella enterica subsp. Enterica serovar enteritidis str. P125109 complexed with magnesium and gluconic acid
34% identity, 100% coverage: 1:381/382 of query aligns to 4:416/417 of 3twbC
- active site: F41 (≠ V34), R44 (= R37), P123 (≠ K115), R152 (≠ K144), Q154 (≠ N146), D164 (≠ E149), D223 (vs. gap), H225 (= H185), E249 (= E209), G274 (= G234), E275 (= E235), L276 (≠ R236), R296 (≠ Q256), H298 (≠ D258), H325 (= H285), P327 (= P287), E352 (= E310)
- binding D-gluconic acid: R152 (≠ K144), Y158 (vs. gap), D223 (vs. gap), H225 (= H185), E275 (= E235), H325 (= H285), D329 (vs. gap), E352 (= E310)
- binding magnesium ion: D223 (vs. gap), E249 (= E209), E275 (= E235)
Sites not aligning to the query:
B5R541 D-galactonate dehydratase family member SEN1436; D-gluconate dehydratase; EC 4.2.1.-; EC 4.2.1.39 from Salmonella enteritidis PT4 (strain P125109) (see paper)
34% identity, 100% coverage: 1:381/382 of query aligns to 6:418/419 of B5R541
- D225 (vs. gap) binding Mg(2+)
- E251 (= E209) binding Mg(2+)
- E277 (= E235) binding Mg(2+)
2qjmA Crystal structure of the k271e mutant of mannonate dehydratase from novosphingobium aromaticivorans complexed with mg and d-mannonate (see paper)
35% identity, 100% coverage: 1:382/382 of query aligns to 1:384/384 of 2qjmA
- active site: G121 (≠ S118), R147 (≠ K144), Q149 (≠ M145), D192 (= D183), H194 (= H185), E218 (= E209), G243 (= G234), E244 (= E235), R265 (≠ Q256), T267 (≠ D258), H294 (= H285), E321 (= E310), W384 (= W382)
- binding d-mannonic acid: N37 (≠ E35), R147 (≠ K144), D192 (= D183), H194 (= H185), E244 (= E235), H294 (= H285), A296 (vs. gap), D298 (≠ P287), E321 (= E310), L371 (≠ N369), W384 (= W382)
- binding magnesium ion: D192 (= D183), E218 (= E209), E244 (= E235)
Query Sequence
>WP_012399852.1 NCBI__GCF_000020045.1:WP_012399852.1
MKITKIETFIVPPRWCFVKIETDEGIVGWGEPVVEGRAHTVAAAVDELSDYLLGKDPRNI
EDHWQVMYRAGFYRGGPISMSAIAGVDQALWDIKGKFHGVPVHTLLGGQVRDRIKVYSWI
GGDRPGDVANNARAVVERGFKAVKMNGSEELQIIDTFDKVQGVINNVSAVREAVGPNVGI
GVDFHGRVHKPMAKVLAKELDPYKLMFIEEPVLSENAEALRDIVNQTSTPIALGERLYSR
WDFKHILAGGYVDIIQPDASHAGGITECRKIASMAEAYDVALALHCPLGPIALATCLQID
AVSYNAFIQEQSLGIHYNQGNDLLDYIKNPEVFKYEDGFVSIPQGPGLGIEVNEEKVREM
AKVGHRWRNPVWRHADGSVAEW
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory