SitesBLAST
Comparing WP_012401370.1 NCBI__GCF_000020045.1:WP_012401370.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1u08A Crystal structure and reactivity of ybdl from escherichia coli identify a methionine aminotransferase function. (see paper)
51% identity, 95% coverage: 21:396/396 of query aligns to 6:382/382 of 1u08A
- binding pyridoxal-5'-phosphate: G95 (= G110), A96 (= A111), T97 (= T112), Y121 (= Y136), N166 (= N181), D198 (= D213), V200 (= V215), Y201 (= Y216), S229 (= S244), K232 (= K247), K240 (= K255)
2o0rA The three-dimensional structure of n-succinyldiaminopimelate aminotransferase from mycobacterium tuberculosis (see paper)
41% identity, 95% coverage: 21:396/396 of query aligns to 4:383/385 of 2o0rA
4wljA High resolution crystal structure of human kynurenine aminotransferase-i in complex with aminooxyacetate (see paper)
35% identity, 94% coverage: 22:395/396 of query aligns to 6:408/413 of 4wljA
- active site: F122 (≠ Y136), D204 (= D213), V206 (= V215), K238 (= K247)
- binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: W15 (≠ F31), G96 (= G110), G97 (≠ A111), Y98 (≠ T112), F122 (≠ Y136), N172 (= N181), N176 (= N185), D204 (= D213), V206 (= V215), Y207 (= Y216), S235 (= S244), K238 (= K247), K246 (= K255), R389 (= R376)
1w7nA Crystal structure of human kynurenine aminotransferase i in pmp form (see paper)
35% identity, 94% coverage: 22:395/396 of query aligns to 6:411/415 of 1w7nA
- active site: F122 (≠ Y136), D207 (= D213), V209 (= V215), K241 (= K247)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G96 (= G110), G97 (≠ A111), Y98 (≠ T112), F122 (≠ Y136), N175 (= N181), D207 (= D213), V209 (= V215), Y210 (= Y216), S238 (= S244), K241 (= K247), K249 (= K255)
1w7mA Crystal structure of human kynurenine aminotransferase i in complex with l-phe (see paper)
35% identity, 94% coverage: 22:395/396 of query aligns to 6:411/415 of 1w7mA
- active site: F122 (≠ Y136), D207 (= D213), V209 (= V215), K241 (= K247)
- binding phenylalanine: W15 (≠ F31), G33 (= G49), Y98 (≠ T112), F122 (≠ Y136), N179 (= N185), R392 (= R376)
- binding pyridoxal-5'-phosphate: G96 (= G110), G97 (≠ A111), Y98 (≠ T112), F122 (≠ Y136), N175 (= N181), N179 (= N185), D207 (= D213), V209 (= V215), Y210 (= Y216), S238 (= S244), K241 (= K247), K249 (= K255)
1w7lA Crystal structure of human kynurenine aminotransferase i (see paper)
35% identity, 94% coverage: 22:395/396 of query aligns to 6:411/415 of 1w7lA
- active site: F122 (≠ Y136), D207 (= D213), V209 (= V215), K241 (= K247)
- binding pyridoxal-5'-phosphate: G96 (= G110), G97 (≠ A111), Y98 (≠ T112), F122 (≠ Y136), N175 (= N181), N179 (= N185), D207 (= D213), V209 (= V215), Y210 (= Y216), S238 (= S244), K241 (= K247), K249 (= K255)
Q16773 Kynurenine--oxoglutarate transaminase 1; Cysteine-S-conjugate beta-lyase; Glutamine transaminase K; GTK; Glutamine--phenylpyruvate transaminase; Kynurenine aminotransferase 1; Kynurenine aminotransferase I; KATI; Kynurenine--oxoglutarate transaminase I; EC 2.6.1.7; EC 4.4.1.13; EC 2.6.1.64 from Homo sapiens (Human) (see paper)
34% identity, 94% coverage: 22:395/396 of query aligns to 9:417/422 of Q16773
- G36 (= G49) binding substrate
- N185 (= N185) binding substrate
- K247 (= K247) modified: N6-(pyridoxal phosphate)lysine
- R398 (= R376) binding substrate
3fvuA Crystal structure of human kynurenine aminotransferase i in complex with indole-3-acetic acid (see paper)
34% identity, 94% coverage: 22:395/396 of query aligns to 6:414/419 of 3fvuA
- active site: F122 (≠ Y136), D210 (= D213), V212 (= V215), K244 (= K247)
- binding glycerol: A74 (≠ S88), S75 (≠ E89), E84 (≠ R98), I85 (≠ Y99)
- binding 1h-indol-3-ylacetic acid: W15 (≠ F31), G33 (= G49), Y60 (= Y74), Y98 (≠ T112), F122 (≠ Y136), K244 (= K247), F275 (= F278), H276 (≠ T279), R395 (= R376)
3fvsB Human kynurenine aminotransferase i in complex with glycerol (see paper)
34% identity, 94% coverage: 22:395/396 of query aligns to 6:414/419 of 3fvsB
Q17CS8 Kynurenine aminotransferase; AeKAT; EC 2.6.1.-; EC 2.6.1.63; EC 2.6.1.7 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (see 2 papers)
33% identity, 98% coverage: 8:395/396 of query aligns to 52:472/477 of Q17CS8
- Y121 (= Y74) binding pyridoxal 5'-phosphate
- AY 158:159 (≠ AT 111:112) binding in other chain
- N241 (= N185) binding in other chain
- Y272 (= Y216) binding in other chain
- S300 (= S244) binding in other chain
- K303 (= K247) modified: N6-(pyridoxal phosphate)lysine
- K311 (= K255) binding in other chain
- R453 (= R376) binding substrate
Sites not aligning to the query:
5verA Mouse kynurenine aminotransferase iii, re-refinement of the PDB structure 3e2z (see paper)
35% identity, 93% coverage: 22:391/396 of query aligns to 4:404/410 of 5verA
- active site: Y119 (= Y136), D206 (= D213), V208 (= V215), K240 (= K247)
- binding calcium ion: E6 (≠ P24)
- binding pyridoxal-5'-phosphate: G93 (= G110), A94 (= A111), Y95 (≠ T112), N174 (= N181), N178 (= N185), D206 (= D213), Y209 (= Y216), S237 (= S244), K240 (= K247), K248 (= K255)
Sites not aligning to the query:
5vepA Mouse kynurenine aminotransferase iii, re-refinement of the PDB structure 3e2f (see paper)
35% identity, 93% coverage: 22:391/396 of query aligns to 4:404/410 of 5vepA
Sites not aligning to the query:
3e2zA Crystal structure of mouse kynurenine aminotransferase iii in complex with kynurenine (see paper)
35% identity, 93% coverage: 22:391/396 of query aligns to 4:404/410 of 3e2zA
3e2yA Crystal structure of mouse kynurenine aminotransferase iii in complex with glutamine (see paper)
35% identity, 93% coverage: 22:391/396 of query aligns to 4:404/410 of 3e2yA
- active site: Y119 (= Y136), D206 (= D213), V208 (= V215), K240 (= K247)
- binding glutamine: W13 (≠ F31), G31 (= G49), Y119 (= Y136), R389 (= R376)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G93 (= G110), A94 (= A111), Y95 (≠ T112), Y119 (= Y136), N174 (= N181), D206 (= D213), Y209 (= Y216), S237 (= S244), K240 (= K247), K248 (= K255)
2r5eA Aedes kynurenine aminotransferase in complex with glutamine (see paper)
33% identity, 95% coverage: 19:395/396 of query aligns to 5:414/419 of 2r5eA
- active site: F125 (≠ Y136), D211 (= D213), V213 (= V215), K245 (= K247)
- binding n~2~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-glutamine: W17 (≠ F31), Q34 (= Q48), Y63 (= Y74), G99 (= G110), A100 (= A111), Y101 (≠ T112), F125 (≠ Y136), N179 (= N181), N183 (= N185), D211 (= D213), Y214 (= Y216), S242 (= S244), K245 (= K247), K253 (= K255), Y276 (≠ F278), F337 (= F331), R395 (= R376)
2r5cB Aedes kynurenine aminotransferase in complex with cysteine (see paper)
33% identity, 95% coverage: 19:395/396 of query aligns to 5:414/419 of 2r5cB
- active site: F125 (≠ Y136), D211 (= D213), V213 (= V215), K245 (= K247)
- binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: W17 (≠ F31), G35 (= G49), G99 (= G110), A100 (= A111), Y101 (≠ T112), F125 (≠ Y136), N179 (= N181), N183 (= N185), D211 (= D213), Y214 (= Y216), S242 (= S244), K245 (= K247), K253 (= K255), F337 (= F331), R395 (= R376)
1yiyA Aedes aegypti kynurenine aminotransferase (see paper)
33% identity, 95% coverage: 19:395/396 of query aligns to 4:413/418 of 1yiyA
- active site: F124 (≠ Y136), D210 (= D213), V212 (= V215), K244 (= K247)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G98 (= G110), A99 (= A111), Y100 (≠ T112), F124 (≠ Y136), N178 (= N181), D210 (= D213), V212 (= V215), Y213 (= Y216), S241 (= S244), K244 (= K247), K252 (= K255)
1v2fA Crystal structure of t.Th hb8 glutamine aminotransferase complex with 3-phenylpropionate (see paper)
36% identity, 95% coverage: 22:396/396 of query aligns to 6:367/368 of 1v2fA
- active site: F112 (≠ Y136), A181 (≠ V203), A183 (≠ G205), K222 (= K247)
- binding hydrocinnamic acid: F15 (= F31), Q32 (= Q48), G33 (= G49), Y57 (= Y74), F112 (≠ Y136), F253 (= F278), R347 (= R376)
- binding pyridoxal-5'-phosphate: G86 (= G110), A87 (= A111), T88 (= T112), F112 (≠ Y136), N163 (= N185), D191 (= D213), V193 (= V215), Y194 (= Y216), S219 (= S244), K222 (= K247), R230 (≠ K255)
1v2eA Crystal structure of t.Th hb8 glutamine aminotransferase complex with a-keto-g-methylthiobutyrate (see paper)
36% identity, 95% coverage: 22:396/396 of query aligns to 6:367/368 of 1v2eA
- active site: F112 (≠ Y136), A181 (≠ V203), A183 (≠ G205), K222 (= K247)
- binding 4-(methylsulfanyl)-2-oxobutanoic acid: F15 (= F31), Q32 (= Q48), G33 (= G49), F112 (≠ Y136), K222 (= K247), F309 (= F331), R347 (= R376)
- binding pyridoxal-5'-phosphate: G86 (= G110), A87 (= A111), T88 (= T112), F112 (≠ Y136), D191 (= D213), V193 (= V215), Y194 (= Y216), S219 (= S244), K222 (= K247), R230 (≠ K255)
1gdeA Crystal structure of pyrococcus protein a-1 e-form (see paper)
32% identity, 90% coverage: 41:396/396 of query aligns to 25:381/388 of 1gdeA
- active site: K232 (= K247)
- binding glutamic acid: F120 (≠ Y136), N170 (= N185), R361 (= R376)
- binding pyridoxal-5'-phosphate: G94 (= G110), A95 (= A111), N96 (≠ T112), F120 (≠ Y136), N166 (= N181), D198 (= D213), Y201 (= Y216), S231 (≠ G246), K232 (= K247), R240 (≠ K255)
Query Sequence
>WP_012401370.1 NCBI__GCF_000020045.1:WP_012401370.1
MNAPHDPTTTTPMTTTPSFPSRLPSVGTTIFTVMSALAVEKNAVNLGQGFPDFDCDPRIV
DAVSNAMREGHNQYPPMAGVAPLRQAISEKISSLYGRRYDATTEITVTAGATQALLTAIL
CAVHPGDEVIVVEPTYDSYLPSIELAGGKPVFVTLDAPDYAIPFDKLAAAITPRTRMILI
NTPHNPTGTVWRAEDMKKLEDIVRGTNVLILSDEVYEHMVYDGAPHESVARYPELAQRSF
VVSSFGKTYHVTGWKVGYVAAPAALMAEFRKVHQFNVFTVNTPMQLGLAHYMQDPAPYLN
LPAFYQKKRDFFRAGLAQSRFKLLPCTGTYFQCVDYSAISDMPEAEFAQWLTSEIGVAAI
PVSAFYHERHESGVVRFCFAKKEDTLATALERLARL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory