SitesBLAST
Comparing WP_012401759.1 NCBI__GCF_000020045.1:WP_012401759.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
34% identity, 94% coverage: 17:550/571 of query aligns to 1:565/565 of 5oc1A
- active site: V339 (≠ G332), N413 (≠ V399), A414 (≠ C400), I499 (= I484), H501 (= H486), A544 (≠ G529), H545 (≠ N530)
- binding 4-methoxybenzoic acid: Y91 (≠ G106), I356 (vs. gap), I390 (≠ P379), F396 (= F385), T412 (≠ S398), I499 (= I484), H501 (= H486), H545 (≠ N530)
- binding flavin-adenine dinucleotide: G8 (= G24), G10 (= G26), N11 (≠ T27), A12 (= A28), E32 (= E48), A33 (= A49), W60 (= W75), P78 (= P93), G80 (= G95), G85 (= G100), S86 (≠ C101), H90 (≠ N105), Y91 (≠ G106), V93 (≠ I108), V230 (≠ T233), S270 (≠ C266), A271 (= A267), G272 (= G268), F500 (= F485), H545 (≠ N530), T546 (= T531), Q547 (≠ N532), I550 (≠ T535)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
33% identity, 94% coverage: 17:550/571 of query aligns to 1:565/565 of 3fimB
- active site: V339 (≠ G332), N413 (≠ V399), A414 (≠ C400), I499 (= I484), H501 (= H486), A544 (≠ G529), H545 (≠ N530)
- binding flavin-adenine dinucleotide: G8 (= G24), N11 (≠ T27), A12 (= A28), E32 (= E48), A33 (= A49), W60 (= W75), P78 (= P93), G80 (= G95), G85 (= G100), S86 (≠ C101), H90 (≠ N105), Y91 (≠ G106), V93 (≠ I108), V230 (≠ T233), S270 (≠ C266), A271 (= A267), F500 (= F485), H501 (= H486), H545 (≠ N530), T546 (= T531), Q547 (≠ N532), I550 (≠ T535)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
34% identity, 93% coverage: 17:546/571 of query aligns to 23:569/578 of 5nccA
- active site: R347 (≠ H329), L420 (≠ V399), I421 (≠ C400), S507 (≠ A477), A509 (≠ N479), G552 (= G529), Q553 (≠ N530)
- binding flavin-adenine dinucleotide: G30 (= G24), G32 (= G26), T33 (= T27), A34 (= A28), L53 (≠ V47), E54 (= E48), A55 (= A49), F74 (≠ I68), W80 (= W75), A98 (≠ P93), G100 (= G95), G105 (= G100), S106 (≠ C101), N110 (= N105), A111 (≠ G106), T112 (≠ M107), L113 (≠ I108), V238 (≠ T233), A278 (= A267), H282 (≠ N271), L286 (= L275), N508 (≠ G478), Q553 (≠ N530), T554 (= T531), G555 (≠ N532), V558 (≠ T535)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
36% identity, 94% coverage: 14:550/571 of query aligns to 10:530/532 of 4mjwA
- active site: I333 (≠ L355), P377 (≠ A397), N378 (≠ S398), V464 (≠ I484), H466 (= H486), V509 (≠ G529), N510 (= N530)
- binding flavin-adenine dinucleotide: G20 (= G24), G22 (= G26), S23 (≠ T27), E44 (= E48), A45 (= A49), W71 (= W75), R89 (= R94), A90 (≠ G95), G95 (= G100), C96 (= C101), H99 (≠ I104), N100 (= N105), S101 (≠ G106), I103 (= I108), R231 (≠ Q232), A232 (≠ T233), T269 (≠ A267), G270 (= G268), D273 (≠ N271), Y465 (≠ F485), H466 (= H486), A500 (= A520), N510 (= N530), P511 (≠ T531), N512 (= N532), V515 (≠ T535)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
36% identity, 94% coverage: 14:547/571 of query aligns to 10:527/527 of 2jbvA
- active site: I333 (≠ L355), P377 (≠ A397), N378 (≠ S398), V464 (≠ I484), H466 (= H486), V509 (≠ G529), N510 (= N530)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G26), S23 (≠ T27), E44 (= E48), A45 (= A49), W71 (= W75), A90 (≠ G95), G95 (= G100), C96 (= C101), H99 (≠ I104), N100 (= N105), S101 (≠ G106), I103 (= I108), R231 (≠ Q232), A232 (≠ T233), T269 (≠ A267), G270 (= G268), D273 (≠ N271), V464 (≠ I484), Y465 (≠ F485), H466 (= H486), D499 (= D519), A500 (= A520), N510 (= N530), P511 (≠ T531), N512 (= N532), V515 (≠ T535)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
36% identity, 94% coverage: 14:550/571 of query aligns to 10:530/530 of 3ljpA
- active site: I333 (≠ L355), P377 (≠ A397), N378 (≠ S398), A464 (≠ I484), H466 (= H486), V509 (≠ G529), N510 (= N530)
- binding dihydroflavine-adenine dinucleotide: G22 (= G26), S23 (≠ T27), E44 (= E48), A45 (= A49), W71 (= W75), R89 (= R94), A90 (≠ G95), G95 (= G100), C96 (= C101), H99 (≠ I104), N100 (= N105), S101 (≠ G106), I103 (= I108), A232 (≠ T233), T269 (≠ A267), D273 (≠ N271), Y465 (≠ F485), H466 (= H486), D499 (= D519), A500 (= A520), N510 (= N530), P511 (≠ T531), N512 (= N532), V515 (≠ T535)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
34% identity, 93% coverage: 17:546/571 of query aligns to 83:636/654 of A0A248QE08
- TA 93:94 (= TA 27:28) binding FAD
- E114 (= E48) binding FAD
- L162 (≠ V97) binding FAD
- S166 (≠ C101) binding FAD
- NATL 170:173 (≠ NGMI 105:108) binding FAD
- V298 (≠ T233) binding FAD
- C432 (≠ S353) binding hexadecanoate
- R451 (≠ H376) binding hexadecanoate
- Y466 (vs. gap) binding hexadecanoate
- Q486 (≠ S398) binding hexadecanoate
- G622 (≠ N532) binding FAD
6yrvAAA structure of fap after illumination at 100k (see paper)
34% identity, 93% coverage: 17:546/571 of query aligns to 7:560/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H376), N499 (≠ G478)
- binding flavin-adenine dinucleotide: G14 (= G24), G16 (= G26), T17 (= T27), A18 (= A28), L37 (≠ V47), E38 (= E48), A39 (= A49), F58 (≠ I68), W64 (= W75), A82 (≠ P93), G89 (= G100), S90 (≠ C101), N94 (= N105), A95 (≠ G106), T96 (≠ M107), L97 (≠ I108), M191 (≠ N202), V222 (≠ T233), C264 (= C266), A265 (= A267), G266 (= G268), H269 (≠ N271), N499 (≠ G478), A534 (= A520), Q544 (≠ N530), T545 (= T531), G546 (≠ N532)
- binding heptadecane: V377 (≠ Q378), G379 (vs. gap), M380 (≠ L380), G386 (vs. gap), T389 (vs. gap), Y390 (vs. gap), F393 (= F385), T408 (= T396), Q410 (≠ S398)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
33% identity, 93% coverage: 15:547/571 of query aligns to 2:451/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G24), G13 (= G26), S14 (≠ T27), A15 (= A28), E35 (= E48), A36 (= A49), W47 (= W75), P65 (= P93), G67 (= G95), V180 (≠ T233), A214 (= A267), G215 (= G268), A218 (≠ N271), T270 (= T325), Y391 (≠ F485), A424 (= A520), I435 (≠ T531), N436 (= N532)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
36% identity, 91% coverage: 19:540/571 of query aligns to 3:498/508 of 4ha6A
- active site: F360 (≠ V399), G361 (≠ C400), H444 (≠ I484), H446 (= H486), G487 (= G529), P488 (≠ N530)
- binding flavin-adenine dinucleotide: G8 (= G24), G10 (= G26), S11 (≠ T27), A12 (= A28), E32 (= E48), A33 (= A49), W58 (= W75), R77 (= R94), G78 (= G95), G83 (= G100), S84 (≠ C101), L87 (≠ I104), H88 (≠ N105), A89 (≠ G106), M90 (= M107), G91 (≠ I108), V218 (≠ T233), A251 (= A267), G252 (= G268), E255 (≠ N271), H445 (≠ F485), A478 (= A520), P488 (≠ N530), I489 (≠ T531), H490 (≠ N532)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (≠ G106), S314 (= S353), H444 (≠ I484), H446 (= H486)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
36% identity, 91% coverage: 19:540/571 of query aligns to 3:498/509 of 3t37A
- active site: F360 (≠ V399), G361 (≠ C400), H444 (≠ I484), H446 (= H486), G487 (= G529), P488 (≠ N530)
- binding flavin-adenine dinucleotide: G8 (= G24), G10 (= G26), S11 (≠ T27), A12 (= A28), E32 (= E48), A33 (= A49), W58 (= W75), R77 (= R94), G78 (= G95), R79 (= R96), G83 (= G100), S84 (≠ C101), H88 (≠ N105), A89 (≠ G106), G91 (≠ I108), R217 (≠ Q232), V218 (≠ T233), A251 (= A267), E255 (≠ N271), H445 (≠ F485), A478 (= A520), P488 (≠ N530), I489 (≠ T531), H490 (≠ N532)
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
32% identity, 93% coverage: 18:549/571 of query aligns to 4:565/566 of 7vzsA
- binding D-glucal: Y6 (= Y20), L22 (= L36), N25 (≠ D39), Y51 (= Y64), I349 (≠ D364), Q356 (≠ P371), E411 (≠ S398), E444 (= E431), W445 (≠ H432), K448 (≠ H435), R499 (≠ G481), N501 (≠ T483), H546 (≠ N530), K563 (≠ R547)
- binding flavin-adenine dinucleotide: G10 (= G24), G12 (= G26), T13 (= T27), S14 (≠ A28), E34 (= E48), A35 (= A49), Y51 (= Y64), F55 (≠ I68), W61 (= W75), R79 (≠ P93), G81 (= G95), G86 (= G100), T87 (≠ C101), N91 (= N105), G92 (= G106), M93 (= M107), A94 (≠ I108), T232 (≠ Q232), A233 (≠ T233), A273 (= A267), G274 (= G268), R277 (≠ N271), F502 (= F485), A536 (= A520), H546 (≠ N530), L547 (≠ T531), V548 (≠ N532), L551 (≠ T535)
Sites not aligning to the query:
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
32% identity, 93% coverage: 18:549/571 of query aligns to 4:565/569 of 4ynuA
- active site: V341 (= V357), F412 (≠ V399), W413 (≠ C400), N501 (≠ T483), H503 (= H486), G545 (= G529), H546 (≠ N530)
- binding flavin-adenine dinucleotide: G12 (= G26), T13 (= T27), S14 (≠ A28), E34 (= E48), A35 (= A49), Y51 (= Y64), F55 (≠ I68), W61 (= W75), R79 (≠ P93), G81 (= G95), G86 (= G100), T87 (≠ C101), N91 (= N105), G92 (= G106), T232 (≠ Q232), A233 (≠ T233), A273 (= A267), G274 (= G268), R277 (≠ N271), F502 (= F485), A536 (= A520), H546 (≠ N530), L547 (≠ T531), V548 (≠ N532), L551 (≠ T535)
- binding D-glucono-1,5-lactone: Y51 (= Y64), E411 (≠ S398), A496 (≠ G478), N497 (= N479), R499 (≠ G481), R499 (≠ G481), N501 (≠ T483), H503 (= H486), H546 (≠ N530)
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
32% identity, 93% coverage: 18:549/571 of query aligns to 5:566/570 of 4yntA
- active site: V342 (= V357), F413 (≠ V399), W414 (≠ C400), N502 (≠ T483), H504 (= H486), G546 (= G529), H547 (≠ N530)
- binding dihydroflavine-adenine dinucleotide: G13 (= G26), T14 (= T27), S15 (≠ A28), E35 (= E48), A36 (= A49), F56 (≠ I68), W62 (= W75), R80 (≠ P93), G82 (= G95), G87 (= G100), T88 (≠ C101), N92 (= N105), G93 (= G106), M94 (= M107), A95 (≠ I108), A234 (≠ T233), A274 (= A267), R278 (≠ N271), F503 (= F485), A537 (= A520), H547 (≠ N530), L548 (≠ T531), V549 (≠ N532), L552 (≠ T535)
8bxlB Patulin synthase from penicillium expansum (see paper)
30% identity, 92% coverage: 18:541/571 of query aligns to 14:581/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G24), G22 (= G26), T23 (= T27), A24 (= A28), E44 (= E48), A45 (= A49), W80 (= W75), G100 (= G95), G105 (= G100), S106 (≠ C101), R109 (≠ I104), N110 (= N105), Y111 (≠ G106), A113 (≠ I108), L253 (≠ Q232), A254 (≠ T233), A288 (= A267), Q292 (≠ N271), F525 (= F485), D559 (= D519), A560 (= A520), H570 (≠ N530), P571 (≠ T531), Q572 (≠ N532), L575 (≠ T535)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
29% identity, 93% coverage: 17:546/571 of query aligns to 15:572/577 of 4h7uA
- active site: A343 (≠ M335), V426 (≠ C400), Y510 (≠ I484), H512 (= H486), A555 (≠ G529), H556 (≠ N530)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G24), G24 (= G26), T25 (= T27), A26 (= A28), E46 (= E48), A47 (= A49), W74 (= W75), G99 (= G100), C100 (= C101), H103 (≠ I104), N104 (= N105), G105 (= G106), V107 (≠ I108), L242 (≠ Q232), V243 (≠ T233), G282 (≠ A267), G283 (= G268), A286 (≠ N271), H512 (= H486), A546 (= A520), H556 (≠ N530), T557 (= T531), Q558 (≠ N532), V561 (≠ T535)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
29% identity, 93% coverage: 17:546/571 of query aligns to 40:597/602 of Q3L245
- N100 (≠ R76) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I104) modified: Tele-8alpha-FAD histidine
- N344 (= N304) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H486) active site, Proton acceptor
- H581 (≠ N530) active site
Sites not aligning to the query:
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
32% identity, 93% coverage: 18:546/571 of query aligns to 2:523/525 of 4udqA
- active site: L331 (= L355), F364 (≠ A397), W365 (≠ S398), V461 (≠ I484), H463 (= H486), A506 (≠ G529), N507 (= N530)
- binding flavin-adenine dinucleotide: G8 (= G24), G10 (= G26), T11 (= T27), A12 (= A28), E32 (= E48), A33 (= A49), W64 (= W75), G88 (= G95), G93 (= G100), G94 (≠ C101), N98 (= N105), M99 (≠ G106), V101 (≠ I108), V229 (≠ T233), T261 (≠ C266), A262 (= A267), W462 (≠ F485), H463 (= H486), A497 (= A520), N507 (= N530), T508 (= T531), N509 (= N532), T512 (= T535)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
32% identity, 93% coverage: 18:546/571 of query aligns to 6:527/531 of E4QP00
- V101 (≠ I104) mutation to H: Abolishes activity.
- M103 (≠ G106) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ T396) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ S398) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ I484) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ F485) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H486) mutation to A: Abolishes activity.
- N511 (= N530) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
3q9tA Crystal structure analysis of formate oxidase (see paper)
29% identity, 93% coverage: 18:549/571 of query aligns to 7:572/577 of 3q9tA
- active site: A335 (≠ M335), D422 (≠ C400), A508 (≠ I484), H510 (= H486), C552 (≠ G529), R553 (≠ N530)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl(2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogendiphosphate: G15 (= G26), T16 (= T27), E37 (= E48), A38 (= A49), W65 (= W75), T85 (vs. gap), R86 (= R94), G87 (= G95), G92 (= G100), S93 (≠ C101), N97 (= N105), Y98 (≠ G106), F99 (≠ M107), T100 (≠ I108), S229 (≠ T233), S265 (≠ C266), Q266 (≠ A267), E270 (≠ N271), F509 (= F485), D542 (= D519), A543 (= A520), R553 (≠ N530), I554 (≠ T531), Q555 (≠ N532), V558 (≠ T535)
Query Sequence
>WP_012401759.1 NCBI__GCF_000020045.1:WP_012401759.1
MSAEKIDGTRSKQSSGEFDYIVIGAGTAGCVVASRLSEDNDVSVLLVEAGGKDNYHWIHI
PVGYLYCIGNPRTDWRYKTSEEPGLNGRALAYPRGRVLGGCSSINGMIYMRGQREDYDDW
ARITGDHSWGWDSVLDAFKKSEDYHGGASDAHGAGGPWRVEKQRLKWDILESFAQAAQQT
GIPATDDFNCGDNTGVGYFDVNQRHGIRWNASKAYLRPAANRKNLTILTNAQTQRLVFKN
QRCSGIEFRVGDEYRVARARHEVILCAGAVNSPQLLELSGIGDINRLAKLGIEVIKDLRG
VGENLQDHLQLRTAFRVDGVRTLNTLSAHWWGKLMIGFEYGLFRSGPMAMAPSQLGVFAK
SDPDDKTLRRPDVQYHVQPLSLERFGEPLHSFDAFTASVCHLRPSSRGSVHITSSDPETA
PRIAPNYLSTEHDLHVAANALRLTRRIVAASALERFRPREVLPGIQFQTEEELRHAAGNV
GTTIFHPVGTCRMGRGDDPDSVVDSRLRVHGVCGLRVVDASIMPVITSGNTNSPTLMIAE
RASEMIRADRMRKDTSAEAYSREVLDVKNAY
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory