SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012402122.1 NCBI__GCF_000020045.1:WP_012402122.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
47% identity, 72% coverage: 11:107/135 of query aligns to 8:104/200 of 6j2lA

query
sites
6j2lA

K

E

V

L

Q

D

W

W

D

E

-

K

A

T

N

D

G

G

L

L

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M

P

P

V

V

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I

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Q

E

H

A

A

S

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S

N

G

D

E

V

V

L

L

M

M

F

L

A

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W

Y

M

M

N

N

R

P

E

E

A

A

L

L

S

D

K

K

T

T

I

I

E

E

T

S

G

G

R

K

A

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Y

F

F

F

S

S

R

R

S

T

R

K

Q

Q

R

R

L

L

W

W

F

I

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K

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G

E

E

E

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S

S

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G

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L

H

N

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L

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D
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D

C

C

D
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D

E

N

D
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D

V

T

V

L

L

L

L

V

K

L

V

A

D

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P

V

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-

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G

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C
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C

H

H

T

K

G

G

R

T

H

S

S

S

C
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C

F

F

binding zinc ion: D79 (= D81), D81 (= D83), D83 (= D85), C96 (= C99), C103 (= C106)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
43% identity, 76% coverage: 7:108/135 of query aligns to 4:104/204 of 7bgnA

query
sites
7bgnA

G

S

W

L

L

L

D

D

K

S

V

V

Q

K

W

W

D

D

A

N

N

K

G

G

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L

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P

V

V

A

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I

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A

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Q

E

N

A

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N

G

D

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M

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N

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E

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A

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S

A

K

T

T

T

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I

E

S

T

S

G

R

R

K

A

A

V

T

Y

F

F

Y

S

S

R

R

S

S

R

R

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S

R

S

L

L

W
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W

F

T

K
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K

G
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G

E

E

E
x
T

S
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S

G

N

H

N

V

F

Q

I

H

N

V

V

H

H

E

D

V

V

R

F

L

L

D
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D

C

C

D
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D

E

R

D
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D

V

S

V

I

L

I

L

Y

K

-

V

L

D

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D

G

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A
x
T

C
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C

H
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H

T

T

G

G

R

A

H

E

S

T

C
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C

F

Y

F

Y

binding adenosine monophosphate: W60 (= W63), K62 (= K65), G63 (= G66), T65 (≠ E68), S66 (= S69), T94 (≠ A98), H96 (= H100)
binding zinc ion: D78 (= D81), D80 (= D83), D82 (= D85), C95 (= C99), C102 (= C106)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
44% identity, 74% coverage: 9:108/135 of query aligns to 8:106/213 of 7bgmA

query
sites
7bgmA

L

L

D

D

K

S

V

V

Q

K

W

W

D

D

A

N

N

K

G

G

L

L

V

A

P

V

V

A

I

I

A

A

Q

Q

E

N

A

V

S

D

T

T

N

G

D

A

V

I

L

L

M

M

F

Q

A

G

W

F

M

A

N

N

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R

E

E

A

A

L

V

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A

K

T

T

T

I

I

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G

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R

K

A

A

V

T

Y

F

F

Y

S

S

R

R

S

S

R

R

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S

R

S

L

L

W

W

F

T

K

K

G

G

E

E

E

T

S

S

G

N

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N

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F

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I

H

N

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V

H

H

E

D

V

V

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F

L

L

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D

C

C

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E

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D

V

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V

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L

I

L

Y

K

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V

L

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C

H

H

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T

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G

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C

F

Y

F

Y

binding zinc ion: D80 (= D81), D82 (= D83), D84 (= D85), C97 (= C99), C104 (= C106)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

6j2lB Crystal structure of bi-functional enzyme (see paper)
41% identity, 76% coverage: 11:112/135 of query aligns to 7:111/185 of 6j2lB

query
sites
6j2lB

K

E

V

L

Q

D

W

W

D

E

-

K

A

T

N

D

G

G

L

L

V

M

P

P

V

V

I

I

A

V

Q

Q

E

H

A

A

S

V

T

S

N

G

D

E

V

V

L

L

M

M

F

L

A

G

W

Y

M

M

N

N

R

P

E

E

A

A

L

L

S

D

K

K

T

T

I

I

E

E

T

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G

G

R

K

A

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V

T

Y

F

F

F

S

S

R

R

S

T

R

K

Q

Q

R

R

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L

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W

F

I

K

K

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G

E

E

E

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S

G

G

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L

H

N

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V

H

V

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S

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R

A

L

P

D
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D

C

C

D
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D

E

N

D
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D

V

T

V

L

L

L

L

V

K

L

V

A

D

N

Q

P

V

I

S

G

G

P

I

S

A

S

C

C

-

F

-

G

H

N

T

T

G

A

R

H

H

Q

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W

C

L

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F

F

L

Q

Y

K

Q

F

L

E

E

binding zinc ion: D78 (= D81), D80 (= D83), D82 (= D85)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

Query Sequence

>WP_012402122.1 NCBI__GCF_000020045.1:WP_012402122.1
MNPSEVGWLDKVQWDANGLVPVIAQEASTNDVLMFAWMNREALSKTIETGRAVYFSRSRQ
RLWFKGEESGHVQHVHEVRLDCDEDVVLLKVDQVSGIACHTGRHSCFFQKFEGSVDSGNW
VAVDPVLKDPEHIYK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory