SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_012406989.1 NCBI__GCF_000020025.1:WP_012406989.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
39% identity, 70% coverage: 2:142/202 of query aligns to 537:679/758 of O14289

query
sites
O14289
V
 
I
S
 
P
E
 
K
V
 
F
K
 
T
T
 
V
V
 
V
S
 
E
G
 
G
R
 
I
G
 
A
I
 
A
P
 
P
L
 
L
V
 
P
G
 
M
N
 
A
D
 
N
I
 
V
D
 
D
T
 
T
D
 
D
R
 
K
I
 
I
I
 
I
P
 
P
A
 
K
R
 
Q
Y
 
F
L
 
L
K
 
K
A
 
T
V
 
I
T
 
K
F
 
R
D
 
T
G
 
G
L
 
L
G
 
G
E
 
Q
G
 
F
A
 
A
F
 
F
I
 
Y
D
 
E
D
 
I
R
 
R
T
 
Y
A
 
D
L
 
A
K
 
D
G
 
G
-
 
K
E
 
E
H
 
I
P
 
P
-
 
D
-
 
F
-
 
V
F
 
L
D
 
N
Q
 
R
P
 
E
Q
 
P
Y
 
Y
Q
 
R
G
 
H
A
 
A
N
 
T
I
 
V
L
 
L
I
 
V
V
 
A
N
 
H
R
 
D
N
 
N
F
 
F
G
 
G
C
 
C
G
 
G
S
 
S
S
 
S
R
 
R
E
 
E
H
 
H
A
 
A
P
 
P
Q
 
W
A
 
A
I
 
L
A
 
N
K
 
D
W
 
F
G
 
G
I
 
I
Q
 
R
A
 
V
L
 
I
I
 
I
G
 
A
E
 
P
S
 
S
F
 
F
A
 
A
E
 
D
I
 
I
F
 
F
F
 
F
G
 
N
N
 
N
C
 
C
V
 
F
A
 
K
M
 
N
G
 
G
I
 
M
P
 
L
C
 
P
L
 
I
T
 
P
A
 
T
D
 
P
A
 
I
A
 
E
T
 
Q
V
 
V
K
 
N
Q
 
D
L
 
M
Q
 
M
K
 
K
L
 
-
V
 
-
A
 
A
A
 
A
N
 
E
P
 
N
Q
 
Q
A
 
V
S
 
K
V
 
F
T
 
S
V
 
V
N
 
D
L
 
L

Sites not aligning to the query:

P9WK95 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
34% identity, 74% coverage: 6:154/202 of query aligns to 5:160/198 of P9WK95

query
sites
P9WK95
K
 
H
T
 
T
V
 
H
S
 
S
G
 
G
R
 
I
G
 
G
I
 
V
P
 
P
L
 
L
V
 
R
G
 
R
N
 
S
D
 
N
I
 
V
D
 
D
T
 
T
D
 
D
R
 
Q
I
 
I
I
 
I
P
 
P
A
 
A
R
 
V
Y
 
F
L
 
L
K
 
K
A
 
R
V
 
V
T
 
T
F
 
R
D
 
T
G
 
G
L
 
F
G
 
E
E
 
D
G
 
G
A
 
L
F
 
F
I
 
A
-
 
G
-
 
W
-
 
R
D
 
S
D
 
D
R
 
P
T
 
A
A
 
F
L
 
V
K
 
L
G
 
N
E
 
L
H
 
S
P
 
P
F
 
F
D
 
D
Q
 
R
P
 
-
Q
 
-
Y
 
-
Q
 
-
G
 
-
A
 
G
N
 
S
I
 
V
L
 
L
I
 
V
V
 
A
N
 
G
R
 
P
N
 
D
F
 
F
G
 
G
C
 
T
G
 
G
S
 
S
S
 
S
R
 
R
E
 
E
H
 
H
A
 
A
P
 
V
Q
 
W
A
 
A
I
 
L
A
 
M
K
 
D
W
 
Y
G
 
G
I
 
F
Q
 
R
A
 
V
L
 
V
I
 
I
G
 
S
E
 
S
S
 
R
F
 
F
A
 
G
E
 
D
I
 
I
F
 
F
F
 
R
G
 
G
N
 
N
C
 
A
V
 
G
A
 
K
M
 
A
G
 
G
I
 
L
P
 
L
C
 
A
L
 
A
T
 
E
A
 
V
D
 
A
A
 
Q
A
 
D
T
 
D
V
 
V
K
 
E
Q
 
L
L
 
L
Q
 
W
K
 
K
L
 
L
V
 
I
A
 
E
A
 
Q
N
 
S
P
 
P
Q
 
G
A
 
L
S
 
E
V
 
I
T
 
T
V
 
A
N
 
N
L
 
L
E
 
Q
-
 
D
-
 
R
-
 
I
-
 
I
-
 
T
-
 
A
-
 
A
-
 
T
-
 
V
T
 
V
L
 
L
Q
 
P
V
 
F
Q
x
K
I
 
I
D
 
D
N
 
D
Y
 
H
T
 
S
A
 
A

Q58667 Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
36% identity, 54% coverage: 17:125/202 of query aligns to 11:112/170 of Q58667

query
sites
Q58667
G
 
G
N
 
D
D
 
D
I
 
V
D
 
D
T
 
T
D
 
D
R
 
A
I
 
I
I
 
I
P
 
P
A
 
G
R
 
P
Y
 
Y
L
 
L
K
x
R
A
x
T
V
 
T
T
 
D
F
 
P
D
 
Y
G
 
E
L
 
L
G
 
A
E
 
S
G
 
H
A
 
C
F
 
M
-
 
A
-
 
G
I
 
I
D
 
D
D
 
E
R
 
N
T
 
-
A
 
-
L
 
-
K
 
-
G
 
-
E
 
-
H
 
-
P
 
-
F
 
F
D
 
P
Q
 
K
P
 
K
Q
 
V
Y
 
K
Q
 
E
G
 
G
A
 
-
N
 
D
I
 
V
L
 
I
I
 
V
V
 
A
N
 
G
R
 
E
N
 
N
F
 
F
G
 
G
C
 
C
G
 
G
S
 
S
S
 
S
R
 
R
E
 
E
H
 
Q
A
 
A
P
 
V
Q
 
I
A
 
A
I
 
I
A
 
K
K
 
Y
W
 
C
G
 
G
I
 
I
Q
 
K
A
 
A
L
 
V
I
 
I
G
 
A
E
 
K
S
 
S
F
 
F
A
 
A
E
 
R
I
 
I
F
 
F
F
 
Y
G
 
R
N
 
N
C
 
A
V
 
I
A
 
N
M
 
V
G
 
G
I
 
L
P
 
I
C
 
P
L
 
I
T
 
I
A
 
A
D
 
N
A
 
T
A
 
D
T
 
E
V
 
I
K
 
K
Q
 
D

2pkpA Crystal structure of 3-isopropylmalate dehydratase (leud)from methhanocaldococcus jannaschii dsm2661 (mj1271) (see paper)
36% identity, 54% coverage: 17:125/202 of query aligns to 11:112/167 of 2pkpA

query
sites
2pkpA
G
 
G
N
 
D
D
|
D
I
 
V
D
 
D
T
 
T
D
 
D
R
 
A
I
 
I
I
 
I
P
 
P
A
 
G
R
 
P
Y
 
Y
L
 
L
K
 
R
A
 
T
V
 
T
T
 
D
F
 
P
D
 
Y
G
 
E
L
 
L
G
 
A
E
 
S
G
 
H
A
 
C
F
 
M
-
 
A
-
 
G
I
 
I
D
 
D
D
 
E
R
 
N
T
 
-
A
 
-
L
 
-
K
 
-
G
 
-
E
 
-
H
 
-
P
 
-
F
 
F
D
 
P
Q
 
K
P
 
K
Q
 
V
Y
 
K
Q
 
E
G
 
G
A
 
-
N
 
D
I
 
V
L
 
I
I
 
V
V
 
A
N
 
G
R
 
E
N
 
N
F
 
F
G
 
G
C
|
C
G
 
G
S
 
S
S
 
S
R
 
R
E
 
E
H
 
Q
A
 
A
P
 
V
Q
 
I
A
 
A
I
 
I
A
 
K
K
 
Y
W
 
C
G
 
G
I
 
I
Q
 
K
A
 
A
L
 
V
I
 
I
G
 
A
E
 
K
S
 
S
F
 
F
A
 
A
E
 
R
I
 
I
F
 
F
F
 
Y
G
 
R
N
 
N
C
 
A
V
 
I
A
 
N
M
 
V
G
 
G
I
 
L
P
 
I
C
 
P
L
 
I
T
 
I
A
 
A
D
 
N
A
 
T
A
 
D
T
 
E
V
 
I
K
 
K
Q
 
D

Query Sequence

>WP_012406989.1 NCBI__GCF_000020025.1:WP_012406989.1
MVSEVKTVSGRGIPLVGNDIDTDRIIPARYLKAVTFDGLGEGAFIDDRTALKGEHPFDQP
QYQGANILIVNRNFGCGSSREHAPQAIAKWGIQALIGESFAEIFFGNCVAMGIPCLTADA
ATVKQLQKLVAANPQASVTVNLETLQVQIDNYTAPVAIGEGTRSTFISGTWDACGQLVAN
ASQVRKTSAKLPYIGWGNIAAS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory