SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012407277.1 NCBI__GCF_000020025.1:WP_012407277.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ij6A Crystal structure of a novel-type phosphoserine phosphatase mutant (h9a) from hydrogenobacter thermophilus tk-6 in complex with l-phosphoserine (see paper)
30% identity, 95% coverage: 3:204/212 of query aligns to 2:202/207 of 4ij6A

query
sites
4ij6A

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active site: R8 (= R9), A9 (≠ H10), N15 (≠ S16), R58 (= R59), E82 (= E83), H150 (= H151)
binding phosphoserine: R8 (= R9), Q21 (≠ C22), R58 (= R59), E82 (= E83), H85 (≠ Y86), H150 (= H151), T151 (≠ K152)

1h2fA Bacillus stearothermophilus phoe (previously known as yhfr) in complex with trivanadate (see paper)
28% identity, 95% coverage: 4:204/212 of query aligns to 3:203/207 of 1h2fA

query
sites
1h2fA

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active site: R8 (= R9), H9 (= H10), N15 (≠ S16), R58 (= R59), E82 (= E83), H150 (= H151)
binding phosphate ion: T3 (= T4), G142 (≠ S143), E143 (≠ G144)
binding trivanadate: R8 (= R9), H9 (= H10), N15 (≠ S16), Q21 (≠ C22), R58 (= R59), E82 (= E83), H150 (= H151), G151 (≠ K152), V152 (≠ A153)

Sites not aligning to the query:

binding phosphate ion: 1, 2

1h2eA Bacillus stearothermophilus phoe (previously known as yhfr) in complex with phosphate (see paper)
28% identity, 95% coverage: 4:204/212 of query aligns to 3:203/207 of 1h2eA

query
sites
1h2eA

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active site: R8 (= R9), H9 (= H10), N15 (≠ S16), R58 (= R59), E82 (= E83), H150 (= H151)
binding phosphate ion: R8 (= R9), H9 (= H10), R58 (= R59), E82 (= E83), H150 (= H151)

6m1xC Crystal structure of phosphoserine phosphatase in complex with 3- phosphoglyceric acid from entamoeba histolytica (see paper)
28% identity, 93% coverage: 5:202/212 of query aligns to 4:196/196 of 6m1xC

query
sites
6m1xC

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binding 3-phosphoglyceric acid: R8 (= R9), H9 (= H10), Q21 (≠ C22), R57 (= R59), E79 (= E83), H146 (= H151), G147 (≠ K152)

P9WIC7 Glucosyl-3-phosphoglycerate phosphatase; Mannosyl-3-phosphoglycerate phosphatase; EC 3.1.3.85; EC 3.1.3.70 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
32% identity, 63% coverage: 5:137/212 of query aligns to 6:137/223 of P9WIC7

query
sites
P9WIC7

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R10 (= R9) mutation to A: Loss of phosphatase activity.
H11 (= H10) active site, Tele-phosphohistidine intermediate; mutation to A: Almost completely abolished phosphatase activity.
N17 (≠ S16) mutation to A: About 5% of wild-type phosphatase activity.
K47 (≠ S46) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
R60 (= R59) mutation to A: Loss of phosphatase activity.

Sites not aligning to the query:

159 H→A: About 5% of wild-type phosphatase activity.
209 L→E: Disrupts dimerization of the enzyme, which exists as a monomer and has lost its ability to perform dephosphorylation.

4pzaB The complex structure of mycobacterial glucosyl-3-phosphoglycerate phosphatase rv2419c with inorganic phosphate (see paper)
32% identity, 63% coverage: 5:137/212 of query aligns to 5:136/217 of 4pzaB

query
sites
4pzaB

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active site: H10 (= H10), R59 (= R59), E83 (= E83)
binding phosphate ion: R9 (= R9), H10 (= H10), R59 (= R59), E83 (= E83)

Sites not aligning to the query:

binding phosphate ion: 158, 159

4qihA The structure of mycobacterial glucosyl-3-phosphoglycerate phosphatase rv2419c complexes with vo3 (see paper)
32% identity, 63% coverage: 5:137/212 of query aligns to 4:135/209 of 4qihA

query
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4qihA

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S

P

D

M

L

R

R

R
|
R

T

A

V

Y

L

D

T

T

A

A

K

V

P

K

L

L

C

G

E

E

A

R

I

T

N

G

I

L

E

V

P

V

Q

R

L

V

R

D

D

T

G

R

L

L

K

R

E
|
E

I

T

N

H

Y

L

G

G

K

D

W

W

E

Q

G

G

K

L

T

T

P

H

E

A

V

Q

I

I

S

D

R

A

E

D

Y

A

H

P

D

G

D

A

Y

R

I

L

R

A

W

W

S

R

A

E

D

D

P

A

A

T

W

W

N

-

A

A

P

P

T

H

G

G

E

E

M

S

A

R

V

V

T

D

I

V

A

A

S

A

R

R

A

S

L

R

Q

P

V

L

I

V

E

A

E

E

I

L

active site: H9 (= H10), R58 (= R59), E82 (= E83)
binding vanadate ion: R8 (= R9), H9 (= H10), Q21 (≠ C22), R58 (= R59)

Sites not aligning to the query:

binding vanadate ion: 157

5zr2C Crystal structure of phosphoserine phosphatase mutant (h9a) from entamoeba histolytica in complex with phosphoserine (see paper)
27% identity, 93% coverage: 5:202/212 of query aligns to 4:196/198 of 5zr2C

query
sites
5zr2C

L

L

Y

I

F

L

L

I

R
|
R

H

A

G

G

Q

E

T

T

E

E

C

W

S

N

R

L

N

L

N

G

S

K

F

I

C
x
Q

G
|
G

S

C

I

T

D

D

S

I

E

E

L

L

T

T

P

P

E

N

G

G

L

I

D

Q

M

Q

A

A

K

N

A

E

F

V

A

A

D

Q

A

Q

Y

I

T

K

S

G

K

-

D

N

W

F

T

D

A

I

I

I

F

Y

C

S

S

S

P

P

M

L

R

H

R
|
R

T

A

V

L

L

I

T

T

A

A

K

Q

P

K

L

I

C

-

E

-

A

A

I

G

N

D

I

K

E

E

P

V

Q

H

L

L

R

I

D

E

G

G

L

M

K

K

E
|
E

I

I

N

P

Y

F

G

G

K

T

W

W

E

E

G

G

K

H

T

T

P

F

E

E

V

E

I

L

S

N

R

G

E

D

Y

I

H

N

D

Y

D

K

Y

K

I

F

R

L

W

S

S

G

A

E

D

D

P

G

A

C

W

P

N

F

A

D

P

S

T

T

G

G

G

M

E

S

M

I

A

A

V

S

T

W

I

S

A

K

S

K

R

N

A

A

L

Q

Q

L

V

L

I

L

E

D

E

L

I

C

K

K

Q

Q

N

N

Y

E

T

N

S

K

G

-

N

T

V

I

L

V

V

C

V

V

S

S

H
|
H

K
x
G

A

A

T

W

I

I

R

K

I

T

L

S

L

I

C

L

S

G

F

L

L

L

G

E

I

M

D

E

V

P

G

T

R

M

F

Y

-

H

R

K

F

F

R

Q

L

L

G

G

C

N

P

-

V

-

A

T

S

G

V

I

S

T

I

T

V

F

E

I

F

F

S

R

S

H

H

G

G

H

P

P

L

V

V

L

K

T

V

S

L

F

A

N

E

S

R

T

S

Q

H

H

L

L

binding phosphoserine: R8 (= R9), Q21 (≠ C22), G22 (= G23), R57 (= R59), E79 (= E83), H146 (= H151), G147 (≠ K152)

1k6mA Crystal structure of human liver 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase (see paper)
30% identity, 84% coverage: 4:181/212 of query aligns to 214:383/432 of 1k6mA

query
sites
1k6mA

T

S

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

Q

E

T

S

E

E

C

L

S
x
N

R

I

N

R

N

G

S

R

F

I

C

G

G

G

S

-

I

-

D

D

S

S

E

G

L

L

T

S

P

V

E

R

G

G

L

K

D

Q

M

Y

A

A

K

Y

A

A

F

L

A

A

D

N

A

F

Y

I

T

Q

S

S

K

Q

D

G

W

I

T

S

A

S

-

L

-

K

I

V

F

F

C

T

S

S

P

R

M

M

R

K

R
|
R

T

T

V

I

L

Q

T

T

A

A

K

-

P

-

L

-

C

-

E

E

A

A

I

L

N

G

I

V

E

P

P

Y

Q

E

L

Q

R

F

D

K

G

A

L

L

K

N

E
|
E

I

I

N

D

Y

A

G

G

K

V

W

C

E

E

G

E

K

M

T

T

P

Y

E

E

V

E

I

I

S

Q

R

E

E

H

Y

Y

H

P

D

E

Y

F

I

A

R

L

W

R

S

D

A

Q

D

D

P

K

A

Y

W

R

N

Y

A

R

P

Y

T

P

G

K

G

G

E

E

M

S

A

Y

V

E

T

D

I

L

A

V

S

Q

R

R

A

L

L

E

Q

P

V

V

I

I

E

M

E

E

I

L

K

E

Q

R

N

-

Y

-

T

-

S

Q

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K

Q

A

A

T

V

I

M

R

R

I

C

L

L

C

A

S

Y

F

F

L

L

G

D

I

K

D

S

V

S

G

E

R

E

F

L

R

P

F

Y

R

-

L

L

G

K

C

C

P

P

V

L

A

H

S

T

V

V

active site: R219 (= R9), H220 (= H10), N226 (≠ S16), R269 (= R59), E289 (= E83), H354 (= H151)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13, 14, 15, 16, 17, 18, 92, 120, 131, 134, 135, 136, 184, 210

1bifA 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase bifunctional enzyme complexed with atp-g-s and phosphate (see paper)
29% identity, 84% coverage: 4:181/212 of query aligns to 214:383/432 of 1bifA

query
sites
1bifA

T

S

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

Q

E

T

S

E

E

C

L

S
x
N

R

L

N

K

N

G

S

R

F

I

C

G

G

G

S

-

I

-

D

D

S

P

E

G

L

L

T

S

P

P

E

R

G

G

L

R

D

E

M

F

A

S

K

K

A

H

F

L

A

A

D

Q

A

F

Y

I

T

S

S

D

K

Q

D

N

W

I

T

K

-

D

-

L

A

K

I

V

F

F

C

T

S

S

P

Q

M

M

R

K

R
|
R

T

T

V

I

L

Q

T

T

A

A

K

-

P

-

L

-

C

-

E

E

A

A

I

L

N

S

I

V

E

P

P

Y

Q

E

L

Q

R

F

D

K

G

V

L

L

K

N

E
|
E

I

I

N

D

Y

A

G

G

K

V

W

C

E

E

G

E

K

M

T

T

P
x
Y

E

E

V

E

I

I

S

Q

R

D

E

H

Y

Y

H

P

D

L

D

E

Y

F

I

A

R

L

W
x
R

S

D

A

Q

D

D

P
x
K

A

Y

W

R

N

Y

A

R

P

Y

T

P

G

K

G

G

E

E

M

S

A
x
Y

V

E

T

D

I

L

A

V

S

Q

R

R

A

L

L

E

Q

P

V

V

I

I

E

M

E

E

I

L

K

E

Q

R

N

-

Y

-

T

-

S

Q

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K

Q

A

A

T

V

I

M

R
|
R

I

C

L

L

C

A

S

Y

F

F

L

L

G

D

I

K

D

A

V

A

G

E

R

E

F

L

R

P

F

Y

R

-

L

L

G

K

C

C

P

P

V

L

A

H

S

T

V

V

active site: R219 (= R9), H220 (= H10), N226 (≠ S16), R269 (= R59), E289 (= E83), H354 (= H151)
binding phosphate ion: R219 (= R9), H220 (= H10), R269 (= R59), E289 (= E83), Y300 (≠ P94), R314 (≠ W108), K318 (≠ P112), Y329 (≠ A123), H354 (= H151), R359 (= R156)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 11, 12, 13, 14, 15, 16, 17, 131, 134, 136, 184, 210, 391

1fbtA The bisphosphatase domain of the bifunctional rat liver 6- phosphofructo-2-kinase/fructose-2,6-bisphosphatase (see paper)
29% identity, 84% coverage: 4:181/212 of query aligns to 2:171/190 of 1fbtA

query
sites
1fbtA

T

S

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

Q

E

T

S

E

E

C

L

S
x
N

R

L

N

R

N

G

S

R

F

I

C

G

G

G

S

-

I

-

D

D

S

S

E

G

L

L

T

S

P

A

E

R

G

G

L

K

D

Q

M

Y

A

A

K

Y

A

A

F

L

A

A

D

N

A

F

Y

I

T

R

S

S

K

Q

D

G

W

I

T

S

A

S

-

L

-

K

I

V

F

W

C

T

S

S

P

H

M

M

R

K

R
|
R

T

T

V

I

L

Q

T

T

A

A

K

-

P

-

L

-

C

-

E

E

A

A

I

L

N

G

I

V

E

P

P

Y

Q

E

L

Q

R

W

D

K

G

A

L

L

K

N

E
|
E

I

I

N

D

Y

A

G

G

K

V

W

C

E

E

G

E

K

M

T

T

P

Y

E

E

V

E

I

I

S

Q

R

E

E

H

Y

Y

H

P

D

E

Y

F

I

A

R

L

W

R

S

D

A

Q

D

D

P

K

A

Y

W

R

N

Y

A

R

P

Y

T

P

G

K

G

G

E

E

M

S

A

Y

V

E

T

D

I

L

A

V

S

Q

R

R

A

L

L

E

Q

P

V

V

I

I

E

M

E

E

I

L

K

E

Q

R

N

Q

Y

-

T

-

S

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K
x
Q

A

A

T

V

I

M

R

R

I

C

L

L

C

A

S

Y

F

F

L

L

G

D

I

K

D

S

V

S

G

D

R

E

F

L

R

P

F

Y

R

-

L

L

G

K

C

C

P

P

V

L

A

H

S

T

V

V

active site: R7 (= R9), H8 (= H10), N14 (≠ S16), R57 (= R59), E77 (= E83), H142 (= H151)
binding phosphate ion: R7 (= R9), H8 (= H10), R57 (= R59), E77 (= E83), H142 (= H151), Q143 (≠ K152)

1tipA The bisphosphatase domain of the bifunctional rat liver 6- phosphofructo-2-kinase/fructose-2,6-bisphosphatase (see paper)
29% identity, 84% coverage: 4:181/212 of query aligns to 3:172/191 of 1tipA

query
sites
1tipA

T

S

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

Q

E

T

S

E

E

C

L

S
x
N

R

L

N

R

N

G

S

R

F
x
I

C

G

G

G

S

-

I

-

D

D

S

S

E

G

L

L

T

S

P

A

E

R

G

G

L

K

D

Q

M

Y

A

A

K

Y

A

A

F

L

A

A

D

N

A

F

Y

I

T

R

S

S

K

Q

D

G

W

I

T

S

A

S

-

L

-

K

I

V

F

W

C

T

S

S

P

H

M

M

R

K

R
|
R

T

T

V

I

L

Q

T

T

A

A

K

-

P

-

L

-

C

-

E

E

A

A

I

L

N

G

I

V

E

P

P

Y

Q

E

L

Q

R

W

D

K

G

A

L

L

K

N

E
|
E

I

I

N

D

Y

A

G

G

K

V

W

C

E

E

G

E

K

M

T

T

P
x
Y

E

E

V

E

I

I

S

Q

R

E

E

H

Y

Y

H

P

D

E

Y

F

I

A

R

L

W
x
R

S

D

A

Q

D

D

P
x
K

A

Y

W

R

N

Y

A

R

P

Y

T

P

G

K

G

G

E

E

M

S

A
x
Y

V

E

T

D

I

L

A

V

S

Q

R

R

A

L

L

E

Q

P

V

V

I

I

E

M

E

E

I

L

K

E

Q

R

N

Q

Y

-

T

-

S

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K

Q

A

A

T

V

I

M

R
|
R

I

C

L

L

C

A

S

Y

F

F

L

L

G

D

I

K

D

S

V

S

G

D

R

E

F

L

R

P

F

Y

R

-

L

L

G

K

C

C

P

P

V

L

A

H

S

T

V

V

active site: R8 (= R9), H9 (= H10), N15 (≠ S16), R58 (= R59), E78 (= E83), H143 (= H151)
binding 6-O-phosphono-beta-D-fructofuranose: I20 (≠ F21), Y89 (≠ P94), R103 (≠ W108), K107 (≠ P112), Y118 (≠ A123), R148 (= R156)

1c81A Michaelis complex of fructose-2,6-bisphosphatase
29% identity, 84% coverage: 4:181/212 of query aligns to 3:172/191 of 1c81A

query
sites
1c81A

T

S

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

Q

E

T

S

E

E

C

L

S
x
N

R

L

N

R

N

G

S

R

F
x
I

C

G

G

G

S

-

I

-

D

D

S

S

E

G

L

L

T

S

P

A

E

R

G

G

L

K

D

Q

M

Y

A

A

K

Y

A

A

F

L

A

A

D

N

A

F

Y

I

T

R

S

S

K

Q

D

G

W

I

T

S

A

S

-

L

-

K

I

V

F

W

C

T

S

S

P

H

M

M

R

K

R
|
R

T

T

V

I

L

Q

T

T

A

A

K

-

P

-

L

-

C

-

E

E

A

A

I

L

N

G

I

V

E

P

P

Y

Q

E

L

Q

R

W

D

K

G

A

L

L

K

N

E
|
E

I

I

N

D

Y

A

G

G

K

V

W

C

E

E

G

E

K

M

T

T

P

Y

E

E

V

E

I

I

S

Q

R

E

E

H

Y

Y

H

P

D

E

Y

F

I

A

R

L

W

R

S

D

A

Q

D

D

P

K

A

Y

W

R

N

Y

A

R

P

Y

T

P

G

K

G

G

E

E

M

S

A

Y

V

E

T

D

I

L

A

V

S

Q

R

R

A

L

L

E

Q

P

V

V

I

I

E

M

E

E

I

L

K

E

Q

R

N

Q

Y

-

T

-

S

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K
x
Q

A

A

T

V

I

M

R
|
R

I

C

L

L

C

A

S

Y

F

F

L

L

G

D

I

K

D

S

V

S

G

D

R

E

F

L

R

P

F

Y

R

-

L

L

G

K

C

C

P

P

V

L

A

H

S

T

V

V

active site: R8 (= R9), H9 (= H10), N15 (≠ S16), R58 (= R59), E78 (= E83), H143 (= H151)
binding 2,5-anhydro-1-deoxy-1-phosphono-6-O-phosphono-D-glucitol: R8 (= R9), H9 (= H10), N15 (≠ S16), I20 (≠ F21), R58 (= R59), E78 (= E83), H143 (= H151), Q144 (≠ K152), R148 (= R156)

1c80A Regulatory complex of fructose-2,6-bisphosphatase
29% identity, 84% coverage: 4:181/212 of query aligns to 3:172/191 of 1c80A

query
sites
1c80A

T

S

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

Q

E

T

S

E

E

C

L

S
x
N

R

L

N

R

N

G

S

R

F
x
I

C

G

G

G

S

-

I

-

D

D

S

S

E

G

L

L

T

S

P

A

E

R

G

G

L

K

D

Q

M

Y

A

A

K

Y

A

A

F

L

A

A

D

N

A

F

Y

I

T

R

S

S

K

Q

D

G

W

I

T

S

A

S

-

L

-

K

I

V

F

W

C

T

S

S

P

H

M

M

R

K

R
|
R

T

T

V

I

L

Q

T

T

A

A

K

-

P

-

L

-

C

-

E

E

A

A

I

L

N

G

I

V

E

P

P

Y

Q

E

L

Q

R

W

D

K

G

A

L

L

K

N

E
|
E

I
|
I

N

D

Y

A

G

G

K

V

W

C

E

E

G

E

K

M

T

T

P
x
Y

E

E

V

E

I

I

S

Q

R

E

E

H

Y

Y

H

P

D

E

Y
x
F

I

A

R

L

W
x
R

S

D

A

Q

D

D

P

K

A

Y

W

R

N

Y

A

R

P
x
Y

T

P

G

K

G

G

E

E

M

S

A
x
Y

V

E

T

D

I

L

A

V

S

Q

R

R

A

L

L

E

Q

P

V

V

I

I

E

M

E

E

I

L

K

E

Q

R

N

Q

Y

-

T

-

S

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K
x
Q

A
|
A

T

V

I

M

R
|
R

I

C

L

L

C

A

S

Y

F

F

L

L

G

D

I

K

D

S

V

S

G

D

R

E

F

L

R

P

F

Y

R

-

L

L

G

K

C

C

P

P

V

L

A

H

S

T

V

V

active site: R8 (= R9), H9 (= H10), N15 (≠ S16), R58 (= R59), E78 (= E83), H143 (= H151)
binding guanosine-5'-triphosphate: I20 (≠ F21), E78 (= E83), I79 (= I84), Y89 (≠ P94), F100 (≠ Y105), R103 (≠ W108), Y112 (≠ P117), Y118 (≠ A123), Q144 (≠ K152), A145 (= A153), R148 (= R156)

1c7zA Regulatory complex of fructose-2,6-bisphosphatase
29% identity, 84% coverage: 4:181/212 of query aligns to 3:172/191 of 1c7zA

query
sites
1c7zA

T

S

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

Q

E

T

S

E

E

C

L

S
x
N

R

L

N

R

N

G

S

R

F

I

C

G

G

G

S

-

I

-

D

D

S

S

E

G

L

L

T

S

P

A

E

R

G

G

L

K

D

Q

M

Y

A

A

K

Y

A

A

F

L

A

A

D

N

A

F

Y

I

T

R

S

S

K

Q

D

G

W

I

T

S

A

S

-

L

-

K

I

V

F

W

C

T

S

S

P

H

M

M

R

K

R
|
R

T

T

V

I

L

Q

T

T

A

A

K

-

P

-

L

-

C

-

E

E

A

A

I

L

N

G

I

V

E

P

P

Y

Q

E

L

Q

R

W

D

K

G

A

L

L

K

N

E
|
E

I

I

N

D

Y

A

G

G

K

V

W

C

E

E

G

E

K

M

T

T

P
x
Y

E

E

V

E

I

I

S

Q

R

E

E

H

Y

Y

H

P

D

E

Y

F

I

A

R

L

W
x
R

S

D

A

Q

D

D

P
x
K

A

Y

W

R

N

Y

A

R

P

Y

T

P

G

K

G

G

E

E

M

S

A
x
Y

V

E

T

D

I

L

A

V

S

Q

R

R

A

L

L

E

Q

P

V

V

I

I

E

M

E

E

I

L

K

E

Q

R

N

Q

Y

-

T

-

S

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K
x
Q

A
|
A

T

V

I

M

R
|
R

I

C

L

L

C

A

S

Y

F

F

L

L

G

D

I

K

D

S

V

S

G

D

R

E

F

L

R

P

F

Y

R

-

L

L

G

K

C

C

P

P

V

L

A

H

S

T

V

V

active site: R8 (= R9), H9 (= H10), N15 (≠ S16), R58 (= R59), E78 (= E83), H143 (= H151)
binding glyceraldehyde-3-phosphate: Y89 (≠ P94), R103 (≠ W108), K107 (≠ P112), Y118 (≠ A123), Q144 (≠ K152), A145 (= A153), R148 (= R156)

P07953 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1; 6PF-2-K/Fru-2,6-P2ase 1; PFK/FBPase 1; 6PF-2-K/Fru-2,6-P2ase liver isozyme; EC 2.7.1.105; EC 3.1.3.46 from Rattus norvegicus (Rat) (see 3 papers)
29% identity, 84% coverage: 4:181/212 of query aligns to 253:422/471 of P07953

query
sites
P07953

T

S

L

I

Y

Y

F

L

L

C

R

R

H
|
H

G

G

Q

E

T

S

E

E

C

L

S

N

R

L

N

R

N

G

S

R

F

I

C
x
G

G

G

S

-

I

-

D

D

S

S

E

G

L

L

T

S

P

A

E

R

G

G

L

K

D

Q

M

Y

A

A

K

Y

A

A

F

L

A

A

D

N

A

F

Y

I

T

R

S

S

K

Q

D

G

W

I

T

S

A

S

-

L

-

K

I

V

F

W

C

T

S

S

P

H

M

M

R

K

R

R

T

T

V

I

L

Q

T

T

A

A

K

-

P

-

L

-

C

-

E

E

A

A

I

L

N

G

I

V

E

P

P

Y

Q

E

L

Q

R

W

D

K

G

A

L

L

K

N

E
|
E

I

I

N

D

Y

A

G

G

K

V

W

C

E

E

G

E

K

M

T

T

P
x
Y

E

E

V

E

I

I

S

Q

R

E

E

H

Y

Y

H

P

D

E

Y
x
F

I
x
A

R
x
L

W
x
R

S

D

A

Q

D

D

P
x
K

A

Y

W

R

N

Y

A

R

P

Y

T

P

G

K

G

G

E

E

M

S

A
x
Y

V

E

T

D

I

L

A

V

S

Q

R

R

A

L

L

E

Q

P

V

V

I

I

E

M

E

E

I

L

K

E

Q

R

N

Q

Y

-

T

-

S

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H

H

K
x
Q

A
|
A

T
x
V

I
x
M

R
|
R

I

C

L

L

C

A

S

Y

F

F

L

L

G

D

I

K

D

S

V

S

G

D

R

E

F

L

R

P

F

Y

R

-

L

L

G

K

C

C

P

P

V

L

A

H

S

T

V

V

H259 (= H10) active site, Tele-phosphohistidine intermediate
G271 (≠ C22) binding beta-D-fructose 2,6-bisphosphate
E328 (= E83) active site, Proton donor/acceptor
Y339 (≠ P94) binding beta-D-fructose 2,6-bisphosphate
FALR 350:353 (≠ YIRW 105:108) binding ATP
R353 (≠ W108) binding beta-D-fructose 2,6-bisphosphate
K357 (≠ P112) binding beta-D-fructose 2,6-bisphosphate
Y368 (≠ A123) binding beta-D-fructose 2,6-bisphosphate
Q394 (≠ K152) binding beta-D-fructose 2,6-bisphosphate
QAVMR 394:398 (≠ KATIR 152:156) binding ATP
R398 (= R156) binding beta-D-fructose 2,6-bisphosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine
33 modified: Phosphoserine; by PKA

P16118 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1; 6PF-2-K/Fru-2,6-P2ase 1; PFK/FBPase 1; 6PF-2-K/Fru-2,6-P2ase liver isozyme; EC 2.7.1.105; EC 3.1.3.46 from Homo sapiens (Human) (see paper)
29% identity, 84% coverage: 4:181/212 of query aligns to 253:422/471 of P16118

query
sites
P16118

T

S

L

I

Y

Y

F

L

L

C

R

R

H

H

G

G

Q

E

T

S

E

E

C

L

S

N

R

I

N

R

N

G

S

R

F

I

C

G

G

G

S

-

I

-

D

D

S

S

E

G

L

L

T

S

P

V

E

R

G

G

L

K

D

Q

M

Y

A

A

K

Y

A

A

F

L

A

A

D

N

A

F

Y

I

T

Q

S

S

K

Q

D

G

W

I

T

S

A

S

-

L

-

K

I

V

F

W

C

T

S

S

P

H

M

M

R

K

R

R

T

T

V

I

L

Q

T

T

A

A

K

-

P

-

L

-

C

-

E

E

A

A

I

L

N

G

I

V

E

P

P

Y

Q

E

L

Q

R

W

D

K

G

A

L

L

K

N

E

E

I

I

N

D

Y

A

G

G

K

V

W

C

E

E

G

E

K

M

T

T

P

Y

E

E

V

E

I

I

S

Q

R

E

E

H

Y

Y

H

P

D

E

Y

F

I

A

R

L

W

R

S

D

A

Q

D

D

P

K

A

Y

W

R

N

Y

A

R

P

Y

T

P

G

K

G

G

E

E

M

S

A

Y

V

E

T

D

I

L

A

V

S

Q

R

R

A

L

L

E

Q

P

V

V

I

I

E

M

E

E

I

L

K

E

Q

R

N

Q

Y

-

T

-

S

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H

H

K

Q

A

A

T

V

I

M

R

R

I

C

L

L

C

A

S

Y

F

F

L

L

G

D

I

K

D

S

V

S

G

D

R

E

F

L

R

P

F

Y

R

-

L

L

G

K

C

C

P

P

V

L

A

H

S

T

V

V

Sites not aligning to the query:

49:57 binding ATP
170:175 binding ATP
430 binding ATP

5htkA Human heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (pfkfb2) (see paper)
28% identity, 84% coverage: 4:181/212 of query aligns to 221:390/425 of 5htkA

query
sites
5htkA

T

T

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

Q

E

T

S

E

E

C

F

S
x
N

R

L

N

L

N

G

S

K

F
x
I

C
x
G

G

G

S

-

I

-

D

D

S

S

E

G

L

L

T

S

P

V

E

R

G

G

L

K

D

Q

M

F

A

A

K

Q

A

A

F

L

A

R

D

K

A

F

Y

L

T

E

S

E

K

Q

D

E

W

I

T

T

-

D

-

L

A

K

I

V

F

W

C

T

S

S

P

Q

M

L

R

K

R
|
R

T

T

V

I

L

Q

T

T

A

A

K

-

P

-

L

-

C

-

E

E

A

S

I

L

N

G

I

V

E

P

P

Y

Q

E

L

Q

R

W

D

K

G

I

L

L

K

N

E
|
E

I

I

N

D

Y

A

G

G

K

V

W

C

E

E

G

E

K

M

T

T

P
x
Y

E

A

V

E

I

I

S

E

R

K

E

R

Y

Y

H

P

D

E

Y

F

I

A

R

L

W
x
R

S

D

A

Q

D

E

P
x
K

A

Y

W

L

N

Y

A

R

P

Y

T

P

G

G

E

E

M

S

A
x
Y

V

Q

T

D

I

L

A

V

S

Q

R

R

A

L

L

E

Q

P

V

V

I

I

E

M

E

E

I

L

K

E

Q

R

N

-

Y

-

T

-

S

Q

G

G

N

N

V

V

L

L

V

V

V

I

S

S

H
|
H

K
x
Q

A

A

T

V

I

M

R
|
R

I

C

L

L

C

A

S

Y

F

F

L

L

G

D

I

K

D

G

V

A

G

D

R

E

F

L

R

P

F

Y

R

-

L

L

G

R

C

C

P

P

V

L

A

H

S

T

V

I

active site: R226 (= R9), H227 (= H10), N233 (≠ S16), R276 (= R59), E296 (= E83), H361 (= H151)
binding 6-O-phosphono-beta-D-fructofuranose: H227 (= H10), I238 (≠ F21), G239 (≠ C22), E296 (= E83), Y307 (≠ P94), R321 (≠ W108), K325 (≠ P112), Y336 (≠ A123), Q362 (≠ K152), R366 (= R156)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 17, 18, 19, 20, 21, 22, 140, 144, 145, 146, 397, 398
binding citrate anion: 48, 61, 72, 99, 100, 167

5hr5A Bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (pfkfb2) (see paper)
28% identity, 84% coverage: 4:181/212 of query aligns to 225:394/424 of 5hr5A

query
sites
5hr5A

T

T

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

Q

E

T

S

E

E

C

F

S
x
N

R

L

N

L

N

G

S

K

F
x
I

C
x
G

G

G

S

-

I

-

D

D

S

S

E

G

L

L

T

S

P

V

E

R

G

G

L

K

D

Q

M

F

A

A

K

Q

A

A

F

L

A

R

D

K

A

F

Y

L

T

E

S

E

K

Q

D

E

W

I

T

A

-

D

-

L

A

K

I

V

F

W

C

T

S

S

P

Q

M

L

R

K

R
|
R

T

T

V

I

L

Q

T

T

A

A

K

-

P

-

L

-

C

-

E

E

A

S

I

L

N

G

I

V

E

T

P

Y

Q

E

L

Q

R

W

D

K

G

I

L

L

K

N

E
|
E

I

I

N

D

Y

A

G

G

K

V

W

C

E

E

G

E

K

M

T

T

P
x
Y

E

A

V

E

I

I

S

Q

R

E

E

Q

Y

Y

H

P

D

D

D

E

Y

F

I

A

R

L

W
x
R

S

D

A

E

D

E

P
x
K

A

Y

W

L

N

Y

A

R

P

Y

T

P

G

G

E

E

M

S

A
x
Y

V

Q

T

D

I

L

A

V

S

Q

R

R

A

L

L

E

Q

P

V

V

I

I

E

M

E

E

I

L

K

E

Q

R

N

-

Y

-

T

-

S

Q

G

G

N

N

V

V

L

L

V

V

V

I

S

S

H
|
H

K
x
Q

A

A

T

V

I

M

R
|
R

I

C

L

L

C

A

S

Y

F

F

L

L

G

D

I

K

D

G

V

A

G

D

R

E

F

L

R

P

F

Y

R

-

L

L

G

R

C

C

P

P

V

L

A

H

S

T

V

I

active site: R230 (= R9), H231 (= H10), N237 (≠ S16), R280 (= R59), E300 (= E83), H365 (= H151)
binding 6-O-phosphono-beta-D-fructofuranose: H231 (= H10), I242 (≠ F21), G243 (≠ C22), E300 (= E83), Y311 (≠ P94), R325 (≠ W108), K329 (≠ P112), Y340 (≠ A123), Q366 (≠ K152), R370 (= R156)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 22, 23, 24, 25, 26, 27, 130, 141, 144, 145, 146, 402
binding citrate anion: 49, 52, 76, 103, 104, 171

O60825 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme; EC 2.7.1.105; EC 3.1.3.46 from Homo sapiens (Human) (see 2 papers)
27% identity, 97% coverage: 4:209/212 of query aligns to 251:453/505 of O60825

query
sites
O60825

T

T

L

I

Y

Y

F

L

L

C

R

R

H

H

G

G

Q

E

T

S

E

E

C

F

S

N

R

L

N

L

N

G

S

K

F

I

C

G

G

G

S

-

I

-

D

D

S

S

E

G

L

L

T

S

P

V

E

R

G

G

L

K

D

Q

M

F

A

A

K

Q

A

A

F

L

A

R

D

K

A

F

Y

L

T

E

S

E

K

Q

D

E

W

I

T

T

-

D

-

L

A

K

I

V

F

W

C

T

S

S

P

Q

M

L

R

K

R

R

T

T

V

I

L

Q

T

T

A

A

K

-

P

-

L

-

C

-

E

E

A

S

I

L

N

G

I

V

E

P

P

Y

Q

E

L

Q

R

W

D

K

G

I

L

L

K

N

E

E

I

I

N

D

Y

A

G

G

K

V

W

C

E

E

G

E

K

M

T

T

P

Y

E

A

V

E

I

I

S

E

R

K

E

R

Y

Y

H

P

D

E

Y

F

I

A

R

L

W

R

S

D

A

Q

D

E

P

K

A

Y

W

L

N

Y

A

R

P

Y

T

P

G

G

E

E

M

S

A

Y

V

Q

T

D

I

L

A

V

S

Q

R

R

A

L

L

E

Q

P

V

V

I

I

E

M

E

E

I

L

K

E

Q

R

N

-

Y

-

T

-

S

Q

G

G

N

N

V

V

L

L

V

V

V

I

S

S

H

H

K

Q

A

A

T

V

I

M

R

R

I

C

L

L

C

A

S

Y

F

F

L

L

G

D

I

K

D

G

V

A

G

D

R

E

F

L

R

P

F

Y

R

-

L

L

G

R

C

C

P

P

V

L

A

H

S

T

V

I

S

F

I

K

V

L

E

T

F

P

S

V

S

A

H

Y

G

G

P

C

L

K

V

V

K

E

V

T

L

I

-

K

-

L

-

N

-

V

-

E

A

A

E

V

R

N

S

T

H

H

L

R

D

D

E

K

R

P

L

T

R

N

N

N

L

F

Sites not aligning to the query:

466 modified: Phosphoserine; by AMPK; S→E: Constitutively active mutant that cannot be phosphorylated and further activated by AMPK.
468 modified: Phosphothreonine
483 modified: Phosphoserine; by BRAF
486 modified: Phosphoserine
493 modified: Phosphoserine

Query Sequence

>WP_012407277.1 NCBI__GCF_000020025.1:WP_012407277.1
MSLTLYFLRHGQTECSRNNSFCGSIDSELTPEGLDMAKAFADAYTSKDWTAIFCSPMRRT
VLTAKPLCEAINIEPQLRDGLKEINYGKWEGKTPEVISREYHDDYIRWSADPAWNAPTGG
EMAVTIASRALQVIEEIKQNYTSGNVLVVSHKATIRILLCSFLGIDVGRFRFRLGCPVAS
VSIVEFSSHGPLVKVLAERSHLDERLRNLAGT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory