SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012408935.1 NCBI__GCF_000020025.1:WP_012408935.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
56% identity, 92% coverage: 27:337/338 of query aligns to 7:316/317 of P14193

query
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P14193

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RQ 102:103 (≠ RA 122:123) binding ATP
K198 (= K219) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R221) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (≠ A223) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (= N225) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (= E228) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (≠ DTGGT 249:253) binding D-ribose 5-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
54% identity, 92% coverage: 27:337/338 of query aligns to 1:299/299 of 1ibsB

query
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1ibsB

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binding methyl phosphonic acid adenosine ester: R96 (= R122), Q97 (≠ A123), R99 (= R125)

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
54% identity, 91% coverage: 30:337/338 of query aligns to 2:297/297 of 1ibsA

query
sites
1ibsA

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binding methyl phosphonic acid adenosine ester: R94 (= R122), Q95 (≠ A123), R97 (= R125), H126 (= H156)
binding sulfate ion: D209 (= D249), T210 (= T250), A211 (≠ G251), T213 (= T253)

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
53% identity, 93% coverage: 24:337/338 of query aligns to 1:315/318 of Q63XL8

query
sites
Q63XL8

M

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T

T

I

F

W

S

R

R

I

I

H

R

E

R

D

G

T

D

S

S

V

V

S

M

S

S

M

L

F

F

RQ 99:100 (≠ RA 122:123) binding ATP

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
55% identity, 91% coverage: 30:337/338 of query aligns to 2:295/295 of 1dkuA

query
sites
1dkuA

L

L

R

K

L

I

F

F

S

S

G

L

S

N

A

S

N

N

L

P

Q

E

L

L

S

A

Q

K

E

E

V

I

A

A

R

D

Y

I

V

V

G

G

M

V

D

Q

L

L

G

G

P

K

M

C

I

S

R

V

K

T

R

R

F

F

A

S

D

D

G

G

E

E

L

V

Y

Q

V

I

Q

N

I

I

Q

E

E

E

S

S

I

I

R

R

G

G

C

C

D

D

V

C

Y

Y

L

I

I

I

Q

Q

P

S

C

T

C

S

Q

D

P

P

V

V

N

N

D

E

H

H

L

I

M

M

E

E

L

L

I

I

M

M

V

V

D

D

A

A

C

L

R

K

R

R

A

A

S

S

A

A

R

K

Q

T

V

I

T

N

A

I

V

V

I

I

P

P

Y

Y

G

G

Y

Y

A

A

R
|
R

A

Q

D

D

R

R

K
|
K

T

A

A

R

G
x
S

R
|
R

E

E

S

P

I

I

T

T

A

A

K

K

L

L

V

F

A

A

N

N

L

L

I

L

T

E

E

T

A

A

G

G

A

A

N

T

R

R

V

V

L

I

A

A

M

L

D

D

L

L

H
|
H

S

A

A

P

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

D

I

I

P

P

F

I

D
|
D

H

H

V

L

Y

M

G

G

S

V

P

P

V

I

L

L

L

G

D

E

Y

Y

L

F

A

E

S

G

K

K

E

N

L

L

P

E

D

D

L

I

V

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

A

T

R

R

A

A

R

R

A

K

F

L

A

A

K

D

K

R

L

L

N

K

D

-

A

A

P

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

Q

-

A

-

H

-

N

-

V

-

A

-

E

-

V

-

L

M

N

N

I

I

I

V

G

G

D

N

V

I

K

E

G

G

K

K

T

T

A

A

V

I

L

L

V

I

D

D

M

I

I

I

D

D

T

T

G

A

G

G

T

T

I

I

A

T

E

L

G

A

A

A

R

N

L

A

L

L

R

V

E

E

E

N

G

G

A

A

R

K

Q

E

V

V

Y

Y

A

A

C

C

A

C

T

T

H

H

A

P

V

V

F

L

S

S

P

G

P

P

A

A

M

V

E

E

R

R

L

I

S

N

G

S

L

T

F

I

E

K

E

E

V

L

I

V

V

V

T

T

N

N

T

S

I

I

P

K

I

L

P

-

E

K

N

I

N

E

R

R

F

F

P

K

Q

Q

L

-

V

-

L

L

S

S

V

V

A

G

N

P

L

L

G

A

E

E

T

A

I

I

W

I

R

R

I

V

H

H

E

E

D

Q

T

Q

S
|
S

V
|
V

S
|
S

S

Y

M

L

F
|
F

binding methyl phosphonic acid adenosine ester: R94 (= R122), H128 (= H156)
binding phosphomethylphosphonic acid adenosyl ester: K98 (= K126), S101 (≠ G129), R102 (= R130), D141 (= D169), S290 (= S332), V291 (= V333), S292 (= S334), F295 (= F337)

6asvC E. Coli prpp synthetase (see paper)
51% identity, 91% coverage: 30:337/338 of query aligns to 2:311/311 of 6asvC

query
sites
6asvC

L

M

R

K

L

L

F

F

S

A

G

G

S

N

A

A

N

T

L

P

Q

E

L

L

S

A

Q

Q

E

R

V

I

A

A

R

N

Y

R

V

L

G

Y

M

T

D

S

L

L

G

G

P

D

M

A

I

A

R

V

K

G

R

R

F

F

A

S

D

D

G

G

E

E

L

V

Y

S

V

V

Q

Q

I

I

Q

N

E

E

S

N

I

V

R

R

G

G

C

G

D

D

V

I

Y

F

L

I

I

I

Q

Q

P

S

C

T

C

C

Q

A

P

P

V

T

N

N

D

D

H

N

L

L

M

M

E

E

L

L

L

V

I

V

M

M

V

V

D

D

A

A

C

L

R

R

A

A

S

S

A

A

R

G

Q

R

V

I

T

T

A

A

V

V

I

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R

R

A

Q

D

D

R

R

K

R

T

V

-

R

A

S

G

A

R

R

E

V

S

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

T

S

E

S

A

V

G

G

A

V

N

D

R

R

V

V

L

L

A

T

M

V

D

D

L

L

H

H

S

A

A

E

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

D

I

V

P

P

F

V

D

D

H

N

V

V

Y

F

G

G

S

S

P

P

V

I

L

L

D

E

Y

D

L

M

A

L

S

Q

K

L

E

N

L

L

P

D

D

N

L

P

V

I

V

V

S

S

P

P

D
|
D

V

I

G

G

V

V

A

V

R

R

A

A

R

R

A

A

F

I

A

A

K

K

K

L

L

L

N

N

D

D

A

T

P

D

L

M

A

A

I

I

D

D

K

K

R

R

Q

P

A

R

H

A

N

N

V

V

A

S

E

Q

V

V

L

M

N

H

I

I

G

G

D

D

V

V

K

A

G

G

K

R

T

D

A

C

V

V

L

L

V

V

D
|
D

M

M

I

I

D
|
D

T
|
T

G
|
G

G

G

T
|
T

I
x
L

A

C

E

K

G

A

A

A

R

E

L

A

L

L

R

K

E

E

E

R

G

G

A

A

R

K

Q

R

V

V

Y

F

A

A

C

Y

A

A

T

T

H

H

A

P

V

I

F

F

S

S

P

G

P

N

A

A

M

A

E

N

R

N

L

L

S

R

S

N

G

S

L

V

F

I

E

D

E

E

V

V

I

V

V

V

T

C

N

D

T

T

I

I

P

P

I

L

P

S

-

D

E

E

N

I

N

K

R

S

F

L

P

P

Q

N

L

V

V

R

V

T

L

L

S

T

V

L

A

S

N

G

L

M

L

L

G

A

E

E

T

A

I

I

W

R

R

R

I

I

H

S

E

N

D

E

T

E

S

S

V

I

S

S

S

A

M

M

F

F

binding phosphate ion: D168 (= D195), D218 (= D245), D219 (= D246), D222 (= D249), T223 (= T250), G224 (= G251), T226 (= T253), L227 (≠ I254)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
51% identity, 91% coverage: 30:337/338 of query aligns to 4:313/315 of P0A717

query
sites
P0A717

L

M

R

K

L

L

F

F

S

A

G

G

S

N

A

A

N

T

L

P

Q

E

L

L

S

A

Q

Q

E

R

V

I

A

A

R

N

Y

R

V

L

G

Y

M

T

D

S

L

L

G

G

P

D

M

A

I

A

R

V

K

G

R

R

F

F

A

S

D

D

G

G

E

E

L

V

Y

S

V

V

Q

Q

I

I

Q

N

E

E

S

N

I

V

R

R

G

G

C

G

D

D

V

I

Y

F

L

I

I

I

Q

Q

P

S

C

T

C

C

Q

A

P

P

V

T

N

N

D

D

H

N

L

L

M

M

E

E

L

L

L

V

I

V

M

M

V

V

D

D

A

A

C

L

R

R

A

A

S

S

A

A

R

G

Q

R

V

I

T

T

A

A

V

V

I

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R

R

A

Q

D

D

R

R

K

R

T

V

-

R

A

S

G

A

R

R

E

V

S

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

T

S

E

S

A

V

G

G

A

V

N

D

R

R

V

V

L

L

A

T

M

V

D
|
D

L

L

H

H

S

A

A

E

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

D

I

V

P

P

F

V

D

D

H

N

V

V

Y

F

G

G

S

S

P

P

V

I

L

L

D

E

Y

D

L

M

A

L

S

Q

K

L

E

N

L

L

P

D

D

N

L

P

V

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

A

V

R

R

A

A

R

R

A

A

F

I

A

A

K

K

K

L

L

L

N

N

D

D

A

T

P

D

L

M

A

A

I

I

D

D

K

K

R

R

Q

P

A

R

H

A

N

N

V

V

A

S

E

Q

V

V

L

M

N

H

I

I

G

G

D

D

V

V

K

A

G

G

K

R

T

D

A

C

V

V

L

L

V

V

D
|
D

M

M

I

I

D
|
D

T

T

G

G

T

T

I

L

A

C

E

K

G

A

A

A

R

E

L

A

L

L

R

K

E

E

E

R

G

G

A

A

R

K

Q

R

V

V

Y

F

A

A

C

Y

A

A

T

T

H

H

A

P

V

I

F

F

S

S

P

G

P

N

A

A

M

A

E

N

R

N

L

L

S

R

S

N

G

S

L

V

F

I

E

D

E

E

V

V

I

V

V

V

T

C

N

D

T

T

I

I

P

P

I

L

P

S

-

D

E

E

N

I

N

K

R

S

F

L

P

P

Q

N

L

V

V

R

V

T

L

L

S

T

V

L

A

S

N

G

L

M

L

L

G

A

E

E

T

A

I

I

W

R

R

R

I

I

H

S

E

N

D

E

T

E

S

S

V

I

S

S

S

A

M

M

F

F

D129 (= D154) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D245) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D246) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (= D249) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
52% identity, 90% coverage: 30:333/338 of query aligns to 2:299/300 of 3dahC

query
sites
3dahC

L

L

R

M

L

V

F

F

S

T

G

G

S

N

A

A

N

N

L

P

Q

A

L

L

S

A

Q

Q

E

E

V

V

A

V

R

K

Y

I

V

L

G

G

M

I

D

P

L

L

G

G

P

K

M

A

I

M

R

V

K

S

R

R

F
|
F

A

S

D
|
D

G

G

E
|
E

L

I

Y

Q

V

V

Q

E

I

I

Q

Q

E

E

S

N

I

V

R

R

G

G

C

K

D

D

V

V

Y

F

L

V

I

L

Q

Q

P

S

C

T

C

C

Q

A

P

P

V

T

N

N

D

D

H

N

L

L

M

M

E

E

L

L

L

M

I

I

M

M

V

V

D

D

A

A

C

L

R

K

R

R

A

A

S

S

A

A

R

G

Q

R

V

I

T

T

A

A

V

A

I

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

A
x
Q

D

D

R

R

K

R

T

P

-

R

A

S

G

A

R

R

E

V

S

A

I

I

T

S

A

A

K

K

L

V

V

V

A

A

N

N

L

M

I

L

T

E

E

I

A

A

G

G

A

V

N

E

R

R

V

I

L

I

A

T

M

M

D

D

L

L

H

H

S

A

A

D

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

D

I

I

P

P

F

V

D

D

H

N

V

I

Y

Y

G

A

S

T

P

P

V

I

L

L

D

G

Y

D

L

L

A

R

S

K

K

Q

E

N

L

Y

P

P

D

D

L

L

V

L

V

V

S

S

P

P

D

D

V

V

G

G

V

V

A

V

R

R

A

A

R

R

A

A

F

L

A

A

K

K

K

Q

L

L

N

N

D

-

A

C

P

D

L

L

A

A

I

I

D

D

K

K

R

R

Q

-

A

-

H

-

N

-

V

-

A

-

E

-

V

V

L

M

N

N

I

I

G

G

D

E

V

V

K

E

G

G

K

R

T

T

A

C

V

V

L

I

V

M

D

D

M

M

I

V

D

D

T

T

G

A

G

G

T

T

I

L

A

C

E

K

G

A

A

A

R

Q

L

V

L

L

R

K

E

E

E

R

G

G

A

A

R

K

Q

Q

V

V

Y

F

A

A

C

Y

A

A

T

T

H

H

A

P

V

V

F

L

S

S

P

G

A

A

M

A

E

D

R

R

L

I

S

A

G

S

L

A

F

L

E

D

E

E

V

L

I

V

V

V

T

T

N

D

T

T

I

I

P

P

I

L

P

S

-

A

E

E

N

S

N

L

R

A

F

C

P

P

Q

K

L

I

V

R

V

A

L

L

S

S

V

S

A

A

N

G

L

L

G

A

E

E

T

T

I

F

W

S

R

R

I

I

H

R

E

R

D

G

T

D

S

S

V

V

binding adenosine monophosphate: F33 (= F61), D35 (= D63), E37 (= E65), R94 (= R122), Q95 (≠ A123)

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
51% identity, 90% coverage: 30:333/338 of query aligns to 3:308/308 of 4s2uA

query
sites
4s2uA

L

M

R

K

L

L

F

F

S

A

G

G

S

N

A

A

N

T

L

P

Q

E

L

L

S

A

Q

Q

E

R

V

I

A

A

R

N

Y

R

V

L

G

Y

M

T

D

S

L

L

G

G

P

D

M

A

I

A

R

V

K

G

R

R

F

F

A

S

D

D

G

G

E

E

L

V

Y

S

V

V

Q

Q

I

I

Q

N

E

E

S

N

I

V

R

R

G

G

C

G

D

D

V

I

Y

F

L

I

I

I

Q

Q

P

S

C

T

C

C

Q

A

P

P

V

T

N

N

D

D

H

N

L

L

M

M

E

E

L

L

L

V

I

V

M

M

V

V

D

D

A

A

C

L

R

R

A

A

S

S

A

A

R

G

Q

R

V

I

T

T

A

A

V

V

I

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R

R

A

Q

D

D

R

R

K

R

T

V

-

R

A

S

G

A

R

R

E

V

S

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

T

S

E

S

A

V

G

G

A

V

N

D

R

R

V

V

L

L

A

T

M

V

D

D

L

L

H

H

S

A

A

E

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

D

I

V

P

P

F

V

D

D

H

N

V

V

Y

F

G

G

S

S

P

P

V

I

L

L

D

E

Y

D

L

M

A

L

S

Q

K

L

E

N

L

L

P

D

D

N

L

P

V

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

A

V

R

R

A

A

R

R

A

A

F

I

A

A

K

K

K

L

L

L

N

N

D

D

A

T

P

D

L

M

A

A

I

I

D

D

K

K

R

R

Q

P

A

R

H

A

N

N

V

V

A

S

E

Q

V

V

L

M

N

H

I

I

G

G

D

D

V

V

K

A

G

G

K

R

T

D

A

C

V

V

L

L

V

V

D

D

M

M

I

I

D
|
D

T

T

G

G

T
|
T

I

L

A

C

E

K

G

A

A

A

R

E

L

A

L

L

R

K

E

E

E

R

G

G

A

A

R

K

Q

R

V

V

Y

F

A

A

C

Y

A

A

T

T

H

H

A

P

V

I

F

F

S

S

P

G

P

N

A

A

M

A

E

N

R

N

L

L

S

R

S

N

G

S

L

V

F

I

E

D

E

E

V

V

I

V

V

V

T

C

N

D

T

T

I

I

P

P

I

L

P

S

-

D

E

E

N

I

N

K

R

S

F

L

P

P

Q

N

L

V

V

R

V

T

L

L

S

T

V

L

A

S

N

G

L

M

L

L

G

A

E

E

T

A

I

I

W

R

R

R

I

I

H

S

E

N

D

E

T

E

S

S

V

I

binding magnesium ion: D223 (= D249), T227 (= T253)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
51% identity, 91% coverage: 30:337/338 of query aligns to 2:305/307 of 7xmvA

query
sites
7xmvA

L

M

R

K

L

L

F

F

S

A

G

G

S

N

A

A

N

T

L

P

Q

E

L

L

S

A

Q

Q

E

R

V

I

A

A

R

N

Y

R

V

L

G

Y

M

T

D

S

L

L

G

G

P

D

M

A

I

A

R

V

K

G

R

R

F
|
F

A

S

D
|
D

G

G

E
|
E

L

V

Y

S

V

V

Q

Q

I

I

Q

N

E

E

S

N

I

V

R

R

G

G

C

G

D

D

V

I

Y

F

L

I

I

I

Q

Q

P

S

C

T

C

C

Q

A

P

P

V

T

N

N

D

D

H

N

L

L

M

M

E

E

L

L

L

V

I

V

M

M

V

V

D

D

A

A

C

L

R

R

A

A

S

S

A

A

R

G

Q

R

V

I

T

T

A

A

V

V

I

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

A

Q

D

D

R
|
R

K

R

T
x
V

-
x
R

A

S

G

A

R

R

E

V

S

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

T

S

E

S

A

V

G

G

A

V

N

D

R

R

V

V

L

L

A

T

M

V

D

D

L

L

H
|
H

S

A

A
x
E

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

D

I

V

P

P

F

V

D

D

H

N

V

V

Y
x
F

G

G

S
|
S

P

P

V

I

L

L

D

E

Y

D

L

M

A

L

S

Q

K

L

E

N

L

L

P

D

D

N

L

P

V

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

A
x
V

R

R

A

A

R

R

A
|
A

F

I

A

A

K

K

K

L

L

L

N

N

D

D

A

T

P

D

L

M

A

A

I

I

D

D

K

K

R

R

Q

Q

A

-

H

-

N

-

V

-

A

-

E

-

V

V

L

M

N

H

I

I

G

G

D

D

V

V

K

A

G

G

K

R

T

D

A

C

V

V

L

L

V

V

D
|
D

M
|
M

I

I

D
|
D

T
|
T

G

G

G
|
G

T
|
T

I

L

A

C

E

K

G

A

A

A

R

E

L

A

L

L

R

K

E

E

E

R

G

G

A

A

R

K

Q

R

V

V

Y

F

A

A

C

Y

A

A

T

T

H

H

A

P

V

I

F

F

S

S

P

G

P

N

A

A

M

A

E

N

R

N

L

L

S

R

S

N

G

S

L

V

F

I

E

D

E

E

V

V

I

V

V

V

T

C

N

D

T

T

I

I

P

P

I

L

P

S

-

D

E

E

N

I

N

K

R

S

F

L

P

P

Q

N

L

V

V

R

V

T

L

L

S

T

V

L

A

S

N

G

L

M

L

L

G

A

E

E

T

A

I

I

W

R

R

R

I

I

H

S

E

N

D

E

T

E

S

S

V

I

S

S

S

A

M

M

F

F

binding adenosine-5'-diphosphate: F33 (= F61), D35 (= D63), E37 (= E65), R94 (= R122), R97 (= R125), H129 (= H156)
binding adenosine monophosphate: R97 (= R125), V99 (≠ T127), R100 (vs. gap), E131 (≠ A158), F145 (≠ Y172), S147 (= S174), V173 (≠ A200), A177 (= A204)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D245), D213 (= D246), M214 (= M247), D216 (= D249), T217 (= T250), G219 (= G252), T220 (= T253)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
51% identity, 91% coverage: 30:337/338 of query aligns to 2:305/307 of 7xmuA

query
sites
7xmuA

L

M

R

K

L

L

F

F

S

A

G

G

S

N

A

A

N

T

L

P

Q

E

L

L

S

A

Q

Q

E

R

V

I

A

A

R

N

Y

R

V

L

G

Y

M

T

D

S

L

L

G

G

P

D

M

A

I

A

R

V

K

G

R

R

F
|
F

A

S

D
|
D

G

G

E
|
E

L

V

Y

S

V

V

Q

Q

I

I

Q

N

E

E

S

N

I

V

R

R

G

G

C

G

D

D

V

I

Y

F

L

I

I

I

Q

Q

P

S

C

T

C

C

Q

A

P

P

V

T

N

N

D

D

H

N

L

L

M

M

E

E

L

L

L

V

I

V

M

M

V

V

D

D

A

A

C

L

R

R

A

A

S

S

A

A

R

G

Q

R

V

I

T

T

A

A

V

V

I

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

A
x
Q

D

D

R
|
R

K

R

T

V

-
x
R

A

S

G

A

R

R

E

V

S

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

T

S

E

S

A

V

G

G

A

V

N

D

R

R

V

V

L

L

A

T

M

V

D

D

L

L

H
|
H

S

A

A
x
E

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

D

I

V

P

P

F

V

D

D

H

N

V

V

Y
x
F

G

G

S
|
S

P

P

V

I

L

L

D

E

Y

D

L

M

A

L

S

Q

K

L

E

N

L

L

P

D

D

N

L

P

V

I

V

V

S

S

P

P

D
|
D

V

I

G

G

V

V

A
x
V

R

R

A

A

R

R

A

A

F

I

A

A

K

K

K

L

L

L

N

N

D

D

A

T

P

D

L

M

A

A

I

I

D

D

K

K

R

R

Q

Q

A

-

H

-

N

-

V

-

A

-

E

-

V

V

L

M

N

H

I

I

G

G

D

D

V

V

K

A

G

G

K

R

T

D

A

C

V

V

L

L

V

V

D
|
D

D

D

M
|
M

I

I

D
|
D

T
|
T

G

G

T

T

I

L

A

C

E

K

G

A

A

A

R

E

L

A

L

L

R

K

E

E

E

R

G

G

A

A

R

K

Q

R

V

V

Y

F

A

A

C

Y

A

A

T

T

H

H

A

P

V

I

F

F

S

S

P

G

P

N

A

A

M

A

E

N

R

N

L

L

S

R

S

N

G

S

L

V

F

I

E

D

E

E

V

V

I

V

V

V

T

C

N

D

T

T

I

I

P

P

I

L

P

S

-

D

E

E

N

I

N

K

R

S

F

L

P

P

Q

N

L

V

V

R

V

T

L

L

S

T

V

L

A

S

N

G

L

M

L

L

G

A

E

E

T

A

I

I

W

R

R

R

I

I

H

S

E

N

D

E

T

E

S

S

V

I

S

S

S

A

M

M

F

F

binding adenosine-5'-diphosphate: F33 (= F61), D35 (= D63), E37 (= E65), R94 (= R122), Q95 (≠ A123), R97 (= R125), R97 (= R125), R100 (vs. gap), H129 (= H156), E131 (≠ A158), F145 (≠ Y172), S147 (= S174), V173 (≠ A200)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D195), D212 (= D245), M214 (= M247), D216 (= D249), T217 (= T250)

P9WKE3 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
48% identity, 94% coverage: 21:337/338 of query aligns to 2:322/326 of P9WKE3

query
sites
P9WKE3

S

S

T

H

G

D

M

W

T

T

D

D

N

N

H

R

R

K

-

N

L

L

R

M

L

L

F

F

S

A

G

G

S

R

A

A

N

H

L

P

Q

E

L

L

S

A

Q

E

E

Q

V

V

A

A

R
x
K

Y

E

V

L

G

D

M

V

D

H

L

V

G

T

P

S

M

Q

I

D

R

A

K

R

R

E

F

F

A

A

D

N

G

G

E

E

L

I

Y

F

V

V

Q

R

I

F

Q

H

E

E

S

S

I

V

R

R

G

G

C

C

D

D

V

A

Y

F

L

V

I

L

Q

Q

P

S

C

C

C

P

Q

A

P

P

V

V

N

N

D

R

H

W

L

L

M

M

E

E

L

Q

L

L

I

I

M

M

V

I

D

D

A

A

C

L

R

K

R

R

A

G

S

S

A

A

R

K

Q

R

V

I

T

T

A

A

V

V

I

M

P

P

Y

F

Y

Y

G

P

Y

Y

A

A

R

R

A

Q

D

D

R

K

K

K

T

H

A

R

G

G

R

R

E

E

S

P

I

I

T

S

A

A

K

R

L

L

V

I

A

A

N

D

L

L

I

L

T

K

E

T

A

A

G

G

A

A

N

D

R

R

V

I

L

V

A

T

M

V

D

D

L

L

H

H

S

T

A

D

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

D

I

G

P

P

F

V

D

D

H

H

V

M

Y

R

G

G

S

Q

P

N

V

L

L

L

L

T

D

G

Y

Y

L

I

A

-

S

R

K

D

E

N

L

Y

P

P

D

D

-

G

-

N

L

M

V

V

S

S

P

P

D

D

V

S

G

G

G

R

V

V

A

R

R

I

A

A

R

E

A

K

F

W

A

A

K

D

K

A

L

L

N

G

D

G

A

V

P

P

L

L

A

A

I

F

I

I

D

H

K

K

R

T

R

R

Q

D

A

P

H

R

N

V

V

P

A

N

E

Q

V

V

L

V

N

S

-

N

-

R

I

V

G

G

D

D

V

V

K

A

G

G

K

R

T

T

A

C

V

V

L

L

V

I

D

D

M

M

I

I

D

D

T

T

G

G

T

T

I

I

A

A

E

G

G

A

A

V

R

A

L

L

R

H

E

N

E

D

G

G

A

A

R

G

Q

D

V

V

Y

I

A

I

C

A

A

A

T

T

H

H

A

G

V

V

F

L

S

S

P

D

P

P

A

A

M

A

E

Q

R

R

L

L

S

A

S

S

G

C

L

G

F

A

E

R

E

E

V

V

I

I

V

V

T

T

N

N

T

T

I

L

P

P

I

I

P

G

E

E

N

D

N

K

R

R

F

F

P

P

Q

Q

L

L

V

T

V

V

L

L

S

S

V

I

A

A

N

P

L

L

G

A

E

S

T

T

I

I

W

R

R

A

I

V

H

F

E

E

D

N

T

G

S

S

V

V

S

T

S

G

M

L

F

F

K29 (≠ R47) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

5t3oA Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus (see paper)
48% identity, 91% coverage: 30:337/338 of query aligns to 2:306/307 of 5t3oA

query
sites
5t3oA

L

L

R

L

L

I

F

F

S

S

G

G

S

Q

A

S

N

N

L

R

Q

P

L

L

S

A

Q

Q

E

A

V

I

A

A

R

E

Y

A

V

L

G

G

M

L

D

P

L

L

G

G

P

K

M

S

I

T

R

T

K

L

R

R

F

F

A

A

D

N

G

D

E

N

L

L

Y

F

V

V

Q

R

I

Y

Q

E

E

E

S
|
S

I

L

R
|
R

G

E

C

G

D

D

V

V

Y

F

L

I

I

V

Q

Q

P

S

C

F

C

V

Q

P

P

P

V

V

N

Q

D

D

H

H

L

L

M

M

E

E

L

L

I

M

M

M

V

V

D

D

A

A

C

A

R

K

R

G

A

A

S

S

A

A

R

A

Q

R

V

V

T

T

A

A

V

V

I

I

P

P

Y

Y

Y

F

G

S

Y

Y

A

A

R
|
R

A
x
S

D

D

R

K

K

K

T
x
D

A

A

G

P

R

R

E

I

S

S

I

I

T

T

A

A

K

R

L

L

V

I

A

A

N

D

L

L

I

L

T

Q

E

T

A

A

G

G

A

A

N

D

R

R

V

V

L

L

A

T

M

M

D

T

L

L

H
|
H

S

S

A

P

Q

Q

I

V

Q

H

G

G

Y

F

F

F

D

K

I

I

P

P

F

V

D

D

H

H

V

L

Y

S

G

A

S

E

P

P

V

V

L

I

L

A

D

N

Y

Y

L

F

A

A

S

T

K

R

-

V

E

D

L

L

P

E

D

N

L

A

V

V

S

A

P

P

D

D

V

A

G

G

G

D

V

L

A

K

R

R

A

A

R

S

A

A

F

L

A

A

K

R

L

L

N

G

D

-

A

L

P

P

L

L

A

A

I

F

I

I

D

D

K

K

R

E

R
|
R

Q

V

A

S

H

D

N

T

V

E

A

V

E

R

V

V

L

R

N

M

I

L

I

V

G

G

D

E

V

V

K

E

G

G

K

K

T

T

A

A

V

L

L

I

V

V

D

D

M

E

I

I

D

S

T

T

G

A

G

G

T

S

I

L

A

V

E

E

G

A

A

V

R

E

L

A

L

L

R

M

E

Q

E

A

G

G

A

A

R

K

Q

E

V

V

Y

Y

A

A

C

A

A

A

T

T

H

H

A

G

V

V

F

Y

S

V

P

G

P

P

A

A

M

L

E

D

R

R

L

I

S

A

S

K

G

S

L

P

F

V

E

K

E

E

V

V

I

A

V

A

T

T

N

D

T

T

I

C

P

P

I

P

P

K

E

E

N

G

N

-

R

-

F

-

P

P

Q

K

L

L

V

R

V

T

L

L

S

T

V

V

A

A

N

P

L

L

L

F

G

A

E

E

T

A

I

I

W

W

R

R

I

I

H

H

E

R

D

G

T

E

S

S

V

V

S

S

M

L

F

F

binding adenosine-5'-diphosphate: R94 (= R122), S95 (≠ A123), D99 (≠ T127), H128 (= H156), R193 (= R221)
binding sulfate ion: S45 (= S73), R47 (= R75)

7pn0A Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus at r32 space group
48% identity, 91% coverage: 30:337/338 of query aligns to 3:307/312 of 7pn0A

query
sites
7pn0A

L

L

R

L

L

I

F

F

S

S

G

G

S

Q

A

S

N

N

L

R

Q

P

L

L

S

A

Q

Q

E

A

V

I

A

A

R

E

Y

A

V

L

G

G

M

L

D

P

L

L

G

G

P

K

M

S

I

T

R

T

K

L

R

R

F

F

A

A

D

N

G

D

E

N

L

L

Y

F

V

V

Q

R

I

Y

Q

E

E

E

S

S

I

L

R

R

G

E

C

G

D

D

V

V

Y

F

L

I

I

V

Q

Q

P

S

C

F

C

V

Q

P

P

P

V

V

N

Q

D

D

H

H

L

L

M

M

E

E

L

L

I

M

M

M

V

V

D

D

A

A

C

A

R

K

R

G

A

A

S

S

A

A

R

A

Q

R

V

V

T

T

A

A

V

V

I

I

P

P

Y

Y

Y

F

G

S

Y

Y

A

A

R
|
R

A
x
S

D

D

R

K

K

K

T

D

A

A

G

P

R

R

E

I

S

S

I

I

T

T

A

A

K

R

L

L

V

I

A

A

N

D

L

L

I

L

T

Q

E

T

A

A

G

G

A

A

N

D

R

R

V

V

L

L

A

T

M

M

D

T

L

L

H
|
H

S

S

A

P

Q

Q

I

V

Q

H

G

G

Y

F

F

F

D

K

I

I

P

P

F

V

D

D

H

H

V

L

Y

S

G

A

S

E

P

P

V

V

L

I

L

A

D

N

Y

Y

L

F

A

A

S

T

K

R

-

V

E

D

L

L

P

E

D

N

L

A

V

V

S

A

P

P

D

D

V

A

G

G

G

D

V

L

A

K

R

R

A

A

R

S

A

A

F

L

A

A

K

R

L

L

N

G

D

-

A

L

P

P

L

L

A

A

I

F

I

I

D

D

K

K

R

E

R

R

Q

V

A

S

H

D

N

T

V

E

A

V

E

R

V

V

L

R

N

M

I

L

I

V

G

G

D

E

V

V

K

E

G

G

K

K

T

T

A

A

V

L

L

I

V

V

D

D

M

E

I

I

D

S

T

T

G

A

G

G

T

S

I

L

A

V

E

E

G

A

A

V

R

E

L

A

L

L

R

M

E

Q

E

A

G

G

A

A

R

K

Q

E

V

V

Y

Y

A

A

C

A

A

A

T

T

H

H

A

G

V

V

F

Y

S

V

P

G

P

P

A

A

M

L

E

D

R

R

L

I

S

A

S

K

G

S

L

P

F

V

E

K

E

E

V

V

I

A

V

A

T

T

N

D

T

T

I

C

P

P

I

P

P

K

E

E

N

G

N

-

R

-

F

-

P

P

Q

K

L

L

V

R

V

T

L

L

S

T

V

V

A

A

N

P

L

L

L

F

G

A

E

E

T

A

I

I

W

W

R

R

I

I

H

H

E

R

D

G

T

E

S

S

V

V

S

S

M

L

F

F

binding adenosine-5'-diphosphate: R95 (= R122), S96 (≠ A123), H129 (= H156)

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
45% identity, 91% coverage: 30:337/338 of query aligns to 4:313/318 of P60891

query
sites
P60891

L

I

R

K

L

I

F

F

S

S

G

G

S

S

A

S

N

H

L

Q

Q

D

L

L

S
|
S

Q

Q

E

K

V

I

A

A

R

D

Y

R

V

L

G

G

M

L

D

E

L

L

G

G

P

K

M

V

I

V

R

T

K

K

R

K

F

F

A

S

D

N

G

Q

E

E

L

T

Y

C

V

V

Q

E

I

I

Q

G

E

E

S

S

I

V

R

R

G

G

C

E

D
|
D

V

V

Y

Y

L

I

I

V

Q

Q

P

S

C

G

C

C

Q

G

P

E

V

I

N

N

D

D

H

N

L

L

M

M

E

E

L

L

I

I

M

M

V

I

D

N

A

A

C

C

R

K

R

I

A

A

S

S

A

A

R

S

Q

R

V

V

T

T

A

A

V

V

I

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R

R

A

Q

D

D

R

K

K

K

T

D

A

K

G

S

R

R

E

A

S

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

N
|
N

L

M

I

L

T

S

E

V

A

A

G

G

A

A

N

D

R

H

V

I

L

I

A

T

M

M

D

D

L
|
L

H

H

S

A

A
x
S

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

D

I

I

P

P

F
x
V

D

D

H
x
N

V

L

Y
|
Y

G

A

S

E

P

P

V

A

L

V

L

L

D

K

Y

W

L

I

A

R

S

E

K

N

-

I

-

S

E

E

L

W

P

R

D

N

L

C

V

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

A

K

R

R

A

V

R

T

A

S

F

I

A

A

K
x
D

K

R

L

L

N

N

D

-

A

V

P

D

L

F

A
|
A

I

L

I

I

D
x
H

K

K

R

E

R

R

Q

K

A

K

H

A

N

N

V

E

A

V

E
x
D

V

R

L

M

N

V

I

L

I

V

G

G

D

D

V

V

K

K

G

D

K

R

T

V

A

A

V

I

L

L

V
|
V

D

D

M

M

I

A

D

D

T

T

G

C

G

G

T

T

I

I

A

C

E
x
H

G

A

A

A

R

D

L

K

L

L

R

L

E

S

E

A

G

G

A

A

R

T

Q

R

V

V

Y

Y

A

A

C

I

A

L

T

T

H

H

A

G

V

I

F

F

S

S

P

G

P

P

A

A

M

I

E

S

R

R

L

I

S

N

G

A

L

C

F

F

E

E

E

A

V

V

I

V

V

V

T

T

N

N

T

T

I

I

P

P

I

Q

P

E

E

D

N

K

N

M

R

K

F

H

-

C

P

S

Q

K

L

I

V

Q

V

V

L

I

S

D

V

I

A

S

N

M

L

I

L

L

G

A

E

E

T

A

I

I

W

R

R

R

I

T

H

H

E

N

D

G

T

E

S

S

V

V

S

S

S

Y

M

L

F

F

S16 (= S42) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (= D78) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (= N140) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (= L155) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ A158) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (≠ F168) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (≠ H170) mutation to H: No effect on catalytic activity.
Y146 (= Y172) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ K207) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (= A215) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ D218) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ E228) to H: in a breast cancer sample; somatic mutation
V219 (= V244) to G: in a breast cancer sample; somatic mutation
H231 (≠ E256) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
45% identity, 91% coverage: 30:337/338 of query aligns to 3:312/316 of 8dbkB

query
sites
8dbkB

L

I

R

K

L

I

F

F

S

S

G

G

S

S

A

S

N

H

L

Q

Q

D

L

L

S

S

Q

Q

E

K

V

I

A

A

R

D

Y

R

V

L

G

G

M

L

D

E

L

L

G

G

P

K

M

V

I

V

R

T

K

K

R

K

F
|
F

A

S

D
x
N

G

Q

E
|
E

L

T

Y

C

V

V

Q

E

I

I

Q

G

E

E

S
|
S

I

V

R
|
R

G

G

C

E

D

D

V

V

Y

Y

L

I

I

V

Q

Q

P

S

C

G

C

C

Q

G

P

E

V

I

N

N

D

D

H

N

L

L

M

M

E

E

L

L

I

I

M

M

V

I

D

N

A

A

C

C

R

K

R

I

A

A

S

S

A

A

R

S

Q

R

V

V

T

T

A

A

V

V

I

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

A
x
Q

D

D

R

K

K

K

T

D

A

K

G

S

R

R

E

A

S

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

I

L

T

S

E

V

A

A

G

G

A

A

N

D

R

H

V

I

L

I

A

T

M

M

D

D

L

L

H
|
H

S

A

A

S

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

D

I

I

P

P

F

V

D

D

H

N

V

L

Y

Y

G

A

S

E

P

P

V

A

L

V

L

L

D

K

Y

W

L

I

A

R

S

E

K

N

-

I

-

S

E

E

L

W

P

R

D

N

L

C

V

T

V

I

V

V

S

S

P

P

D
|
D

V

A

G
|
G

G

G

V

A

A

K

R

R

A

V

R

T

A

S

F

I

A

A

K

D

K

R

L

L

N

N

D

-

A

V

P

D

L

F

A

A

I

L

I

I

D

H

K
|
K

R

E

R
|
R

Q

K

A

K

H

A

N
|
N

V

E

A

V

E

D

V

R

L

M

N

V

I

L

I

V

G

G

D

D

V

V

K

K

G

D

K

R

T

V

A

A

V

I

L

L

V

V

D
|
D

M

M

I

A

D
|
D

T
|
T

G
x
C

G
|
G

T
|
T

I

I

A

C

E

H

G

A

A

A

R

D

L

K

L

L

R

L

E

S

E

A

G

G

A

A

R

T

Q

R

V

V

Y

Y

A

A

C

I

A

L

T

T

H

H

A

G

V

I

F

F

S

S

P

G

P

P

A

A

M

I

E

S

R

R

L

I

S

N

G

A

L

C

F

F

E

E

E

A

V

V

I

V

V

V

T

T

N

N

T

T

I

I

P

P

I

Q

P

E

E

D

N

K

N

M

R

K

F

H

-

C

P

S

Q

K

L

I

V

Q

V

V

L

I

S

D

V

I

A

S

N

M

L

I

L

L

G

A

E

E

T

A

I

I

W

R

R

R

I

T

H

H

E

N

D

G

T

E

S

S

V

V

S

S

S

Y

M

L

F

F

binding adenosine monophosphate: R95 (= R122), Q96 (≠ A123), N199 (= N225)
binding adenosine-5'-triphosphate: F34 (= F61), N36 (≠ D63), E38 (= E65)
binding phosphate ion: S46 (= S73), R48 (= R75)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H156), D170 (= D195), G172 (= G197), K193 (= K219), R195 (= R221), D219 (= D245), D220 (= D246), D223 (= D249), T224 (= T250), C225 (≠ G251), G226 (= G252), T227 (= T253)

8dbeA Human prps1 with adp; hexamer (see paper)
45% identity, 91% coverage: 30:337/338 of query aligns to 3:312/316 of 8dbeA

query
sites
8dbeA

L

I

R

K

L

I

F

F

S

S

G

G

S

S

A

S

N

H

L

Q

Q

D

L

L

S

S

Q

Q

E

K

V

I

A

A

R

D

Y

R

V

L

G

G

M

L

D

E

L

L

G

G

P

K

M

V

I

V

R

T

K

K

R

K

F
|
F

A

S

D
x
N

G

Q

E
|
E

L

T

Y

C

V

V

Q

E

I

I

Q

G

E

E

S

S

I

V

R

R

G

G

C

E

D

D

V

V

Y

Y

L

I

I

V

Q

Q

P

S

C

G

C

C

Q

G

P

E

V

I

N

N

D

D

H

N

L

L

M

M

E

E

L

L

I

I

M

M

V

I

D

N

A

A

C

C

R

K

R

I

A

A

S

S

A

A

R

S

Q

R

V

V

T

T

A

A

V

V

I

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

A
x
Q

D

D

R
x
K

K
|
K

T
x
D

A

K

G
x
S

R
|
R

E

A

S

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

I

L

T

S

E

V

A

A

G

G

A

A

N

D

R

H

V

I

L

I

A

T

M

M

D

D

L

L

H
|
H

S

A

A

S

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

D

I

I

P

P

F

V

D
|
D

H

N

V

L

Y
|
Y

G

A

S

E

P

P

V

A

L

V

L

L

D

K

Y

W

L

I

A

R

S

E

K

N

-

I

-

S

E

E

L

W

P

R

D

N

L

C

V

T

V

I

V

V

S

S

P

P

D
|
D

V

A

G

G

V

A

A

K

R

R

A

V

R

T

A

S

F

I

A

A

K

D

K

R

L

L

N

N

D

-

A

V

P

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

Q

K

A

K

H

A

N

N

V

E

A

V

E

D

V

R

L

M

N

V

I

L

I

V

G

G

D

D

V

V

K

K

G

D

K

R

T

V

A

A

V

I

L

L

V

V

D
|
D

M

M

I

A

D
|
D

T
|
T

G

C

G
|
G

T
|
T

I

I

A

C

E

H

G

A

A

A

R

D

L

K

L

L

R

L

E

S

E

A

G

G

A

A

R

T

Q

R

V

V

Y

Y

A

A

C

I

A

L

T

T

H

H

A

G

V

I

F

F

S

S

P

G

P

P

A

A

M

I

E

S

R

R

L

I

S

N

G

A

L

C

F

F

E

E

E

A

V

V

I

V

V

V

T

T

N

N

T

T

I

I

P

P

I

Q

P

E

E

D

N

K

N

M

R

K

F

H

-

C

P

S

Q

K

L

I

V

Q

V

V

L

I

S

D

V

I

A

S

N

M

L

I

L

L

G

A

E

E

T

A

I

I

W

R

R

R

I

T

H

H

E

N

D

G

T

E

S
|
S

V
|
V

S
|
S

S

Y

M

L

F
|
F

binding adenosine-5'-diphosphate: F34 (= F61), N36 (≠ D63), E38 (= E65), R95 (= R122), Q96 (≠ A123), K98 (≠ R125), K99 (= K126), D100 (≠ T127), S102 (≠ G129), R103 (= R130), H129 (= H156), D142 (= D169), Y145 (= Y172), S307 (= S332), V308 (= V333), S309 (= S334), F312 (= F337)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H156), D170 (= D195), D219 (= D245), D220 (= D246), D223 (= D249), T224 (= T250), G226 (= G252), T227 (= T253)

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
45% identity, 91% coverage: 30:337/338 of query aligns to 2:305/305 of 2hcrA

query
sites
2hcrA

L

I

R

K

L

I

F

F

S

S

G

G

S

S

A

S

N

H

L

Q

Q

D

L

L

S

S

Q

Q

E

K

V

I

A

A

R

D

Y

R

V

L

G

G

M

L

D

E

L

L

G

G

P

K

M

V

I

V

R

T

K

K

R

K

F

F

A

S

D

N

G

Q

E

E

L

T

Y

C

V

V

Q

E

I

I

Q

G

E

E

S

S

I

V

R

R

G

G

C

E

D

D

V

V

Y

Y

L

I

I

V

Q

Q

P

S

C

G

C

C

Q

G

P

E

V

I

N

N

D

D

H

N

L

L

M

M

E

E

L

L

I

I

M

M

V

I

D

N

A

A

C

C

R

K

R

I

A

A

S

S

A

A

R

S

Q

R

V

V

T

T

A

A

V

V

I

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

A
x
Q

D

D

R
x
K

K

K

T

D

A

K

G

S

R

R

E

A

S

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

I

L

T

S

E

V

A

A

G

G

A

A

N

D

R

H

V

I

L

I

A

T

M

M

D

D

L

L

H
|
H

S

A

A

S

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

D

I

I

P

P

F

V

D

D

H

N

V

L

Y

Y

G

A

S

E

P

P

V

A

L

V

L

L

D

K

Y

W

L

I

A

R

S

E

K

N

-

I

-

S

E

E

L

W

P

R

D

N

L

C

V

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

A

K

R

R

A

V

R

T

A

S

F

I

A

A

K

D

K

R

L

L

N

N

D

-

A

V

P

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

Q

-

A

-

H

-

N

-

V

-

A

-

E

D

V

R

L

M

N

V

I

L

I

V

G

G

D

D

V

V

K

K

G

D

K

R

T

V

A

A

V

I

L

L

V

V

D

D

M

M

I

A

D

D

T

T

G

C

G

G

T

T

I

I

A

C

E

H

G

A

A

A

R

D

L

K

L

L

R

L

E

S

E

A

G

G

A

A

R

T

Q

R

V

V

Y

Y

A

A

C

I

A

L

T

T

H

H

A

G

V

I

F

F

S

S

P

G

P

P

A

A

M

I

E

S

R

R

L

I

S

N

G

A

L

C

F

F

E

E

E

A

V

V

I

V

V

V

T

T

N

N

T

T

I

I

P

P

I

Q

P

E

E

D

N

K

N

M

R

K

F

H

-

C

P

S

Q

K

L

I

V

Q

V

V

L

I

S

D

V

I

A

S

N

M

L

I

L

L

G

A

E

E

T

A

I

I

W

R

R

R

I

T

H

H

E

N

D

G

T

E

S

S

V

V

S

S

S

Y

M

L

F

F

binding adenosine monophosphate: R94 (= R122), Q95 (≠ A123), K97 (≠ R125), H128 (= H156)

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
46% identity, 88% coverage: 30:328/338 of query aligns to 3:298/299 of 6nfeB

query
sites
6nfeB

L

M

R

M

L

I

F

F

S

T

G

G

S

N

A

A

N

N

L

P

Q

E

L

L

S

A

Q

L

E

K

V

I

A

S

R

S

Y

H

V

L

G

Q

M

I

D

P

L

I

G

G

P

K

M

A

I

T

R

V

K

G

R

T

F
|
F

A

S

D
|
D

G

G

E
|
E

L

T

Y

M

V

V

Q

E

I

I

Q

L

E

E

S

N

I

V

R

R

G

G

C

K

D

D

V

V

Y

F

L

V

I

L

Q

Q

P

S

C

T

C

C

Q

A

P

P

V

A

N

N

D

N

H

N

L

L

M

M

E

E

L

L

I

I

M

M

V

A

D

D

A

A

C

L

R

R

A

S

S

S

A

A

R

G

Q

R

V

I

T

T

A

A

V

V

I

V

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

A
x
Q

D

D

R

R

K

R

T

V

-

R

A

S

G

A

R

R

E

V

S

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

M

I

M

T

A

E

S

A

V

G

G

A

I

N

C

R

R

V

V

L

L

A

T

M

V

D

D

L

L

H
|
H

S

A

A

D

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

Y

I

M

P

P

F

V

D

D

H

N

V

V

Y

Y

G

S

S

T

P

P

V

V

L

L

D

E

Y

D

L

I

A

T

S

K

K

Q

E

K

L

L

P

N

D

N

L

I

V

M

V

I

V

V

S

S

P

P

D

D

V

V

G

G

V

V

A

V

R

R

A

A

R

R

A

A

F

V

A

A

K

K

K

R

L

L

N

N

D

D

A

A

P

E

L

L

A

S

I

I

D

D

K

K

R

R

Q

-

A

-

H

-

N

-

V

-

A

S

E

E

V

V

L

M

N

H

I

I

G

G

D

E

V

P

K

A

G

N

K

K

T

N

A

C

V

I

L

I

V

V

D
|
D

M
x
I

I

V

D
|
D

T
|
T

G
x
A

G

G

T
|
T

I

L

A

C

E

T

G

A

A

A

R

H

L

E

L

L

R

K

E

K

E

N

G

G

A

A

R

K

Q

S

V

V

Y

R

A

A

C

Y

A

I

T

T

H

H

A

P

V

V

F

L

S

S

P

G

P

P

A

A

M

V

E

N

R

N

L

I

S

K

S

H

G

S

L

G

F

L

E

D

E

E

V

V

I

V

V

V

T

T

N

D

T

T

I

I

P

P

I

L

P

S

-

A

E

E

N

A

N

Q

R

N

F

C

P

E

Q

K

L

I

V

R

V

V

L

V

S

S

V

L

A

A

N

D

L

M

L

L

G

A

E

Q

T

A

I

I

W

K

R

R

I

V

H

N

binding adenosine-5'-diphosphate: F34 (= F61), D36 (= D63), E38 (= E65), R95 (= R122), Q96 (≠ A123), H130 (= H156)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H156), D214 (= D245), D215 (= D246), I216 (≠ M247), D218 (= D249), T219 (= T250), A220 (≠ G251), T222 (= T253)

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
46% identity, 88% coverage: 30:328/338 of query aligns to 3:297/298 of 6nfeA

query
sites
6nfeA

L

M

R

M

L

I

F

F

S

T

G

G

S

N

A

A

N

N

L

P

Q

E

L

L

S

A

Q

L

E

K

V

I

A

S

R

S

Y

H

V

L

G

Q

M

I

D

P

L

I

G

G

P

K

M

A

I

T

R

V

K

G

R

T

F
|
F

A

S

D
|
D

G

G

E
|
E

L

T

Y

M

V

V

Q

E

I

I

Q

L

E

E

S

N

I

V

R

R

G

G

C

K

D

D

V

V

Y

F

L

V

I

L

Q

Q

P

S

C

T

C

C

Q

A

P

P

V

A

N

N

D

N

H

N

L

L

M

M

E

E

L

L

I

I

M

M

V

A

D

D

A

A

C

L

R

R

A

S

S

S

A

A

R

G

Q

R

V

I

T

T

A

A

V

V

I

V

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

A
x
Q

D

D

R
|
R

K

R

T
x
V

-

R

A

S

G

A

R

R

E

V

S

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

M

I

M

T

A

E

S

A

V

G

G

A

I

N

C

R

R

V

V

L

L

A

T

M

V

D

D

L

L

H
|
H

S

A

A

D

Q

Q

I

I

Q

Q

G

G

Y

F

F

F

D

Y

I

M

P

P

F

V

D

D

H

N

V

V

Y
|
Y

G

S

S

T

P

P

V

V

L

L

D

E

Y

D

L

I

A

T

S

K

K

Q

E

K

L

L

P

N

D

N

L

I

V

M

V

I

V

V

S

S

P

P

D

D

V

V

G

G

V

V

A

V

R
|
R

A

A

R

R

A
|
A

F

V

A

A

K
|
K

K

R

L

L

N

N

D

D

A

A

P

E

L

L

A

S

I

I

D

D

K

K

R

R

Q

-

A

-

H

-

N

-

V

-

A

-

E

E

V

V

L

M

N

H

I

I

G

G

D

E

V

P

K

A

G

N

K

K

T

N

A

C

V

I

L

I

V

V

D
|
D

M
x
I

I

V

D
|
D

T
|
T

G
x
A

G

G

T
|
T

I

L

A

C

E

T

G

A

A

A

R

H

L

E

L

L

R

K

E

K

E

N

G

G

A

A

R

K

Q

S

V

V

Y

R

A

A

C

Y

A

I

T

T

H

H

A

P

V

V

F

L

S

S

P

G

P

P

A

A

M

V

E

N

R

N

L

I

S

K

S

H

G

S

L

G

F

L

E

D

E

E

V

V

I

V

V

V

T

T

N

D

T

T

I

I

P

P

I

L

P

S

-

A

E

E

N

A

N

Q

R

N

F

C

P

E

Q

K

L

I

V

R

V

V

L

V

S

S

V

L

A

A

N

D

L

M

L

L

G

A

E

Q

T

A

I

I

W

K

R

R

I

V

H

N

binding adenosine-5'-diphosphate: F34 (= F61), D36 (= D63), E38 (= E65), R95 (= R122), Q96 (≠ A123), H130 (= H156)
binding adenosine monophosphate: R98 (= R125), V100 (≠ T127), Y146 (= Y172), R175 (= R201), A178 (= A204), K181 (= K207)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H156), D213 (= D245), D214 (= D246), I215 (≠ M247), D217 (= D249), T218 (= T250), A219 (≠ G251), T221 (= T253)

Query Sequence

>WP_012408935.1 NCBI__GCF_000020025.1:WP_012408935.1
MNSNRGSAVLSSATFKVQASSTGMTDNHRLRLFSGSANLQLSQEVARYVGMDLGPMIRKR
FADGELYVQIQESIRGCDVYLIQPCCQPVNDHLMELLIMVDACRRASARQVTAVIPYYGY
ARADRKTAGRESITAKLVANLITEAGANRVLAMDLHSAQIQGYFDIPFDHVYGSPVLLDY
LASKELPDLVVVSPDVGGVARARAFAKKLNDAPLAIIDKRRQAHNVAEVLNIIGDVKGKT
AVLVDDMIDTGGTIAEGARLLREEGARQVYACATHAVFSPPAMERLSSGLFEEVIVTNTI
PIPENNRFPQLVVLSVANLLGETIWRIHEDTSVSSMFR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory