SitesBLAST
Comparing WP_012409334.1 NCBI__GCF_000020025.1:WP_012409334.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
53% identity, 98% coverage: 8:386/387 of query aligns to 14:390/392 of P33221
- EL 22:23 (= EL 16:17) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- E82 (= E76) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- R114 (= R108) binding ATP
- K155 (= K149) binding ATP
- SSGKGQ 160:165 (= SSGKGQ 154:159) binding ATP
- G162 (= G156) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
- K179 (≠ N173) modified: N6-acetyllysine
- EGVV 195:198 (≠ EEFI 189:192) binding ATP
- E203 (= E197) binding ATP
- E267 (= E262) binding Mg(2+)
- E279 (= E274) binding Mg(2+)
- D286 (= D281) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- K355 (= K351) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- RR 362:363 (= RR 358:359) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
53% identity, 98% coverage: 8:386/387 of query aligns to 13:387/389 of 1kjiA
- active site: E114 (≠ D109), K154 (= K149), S159 (= S154), G161 (= G156), E264 (= E262), E276 (= E274), D283 (= D281), T284 (= T282), R360 (= R359)
- binding phosphomethylphosphonic acid adenylate ester: R113 (= R108), I152 (≠ V147), K154 (= K149), S159 (= S154), S160 (= S155), G161 (= G156), Q164 (= Q159), E192 (= E189), V195 (≠ I192), E200 (= E197), Q222 (= Q220), E264 (= E262), F266 (= F264), E276 (= E274)
- binding magnesium ion: E264 (= E262), E276 (= E274)
1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
53% identity, 98% coverage: 8:386/387 of query aligns to 13:387/389 of 1ez1A
- active site: E114 (≠ D109), K154 (= K149), S159 (= S154), G161 (= G156), E264 (= E262), E276 (= E274), D283 (= D281), T284 (= T282), R360 (= R359)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R108), I152 (≠ V147), K154 (= K149), S159 (= S154), S160 (= S155), G161 (= G156), E192 (= E189), V194 (≠ F191), V195 (≠ I192), F197 (= F194), E200 (= E197), Q222 (= Q220), E264 (= E262), F266 (= F264), E276 (= E274)
- binding glycinamide ribonucleotide: G20 (= G15), E21 (= E16), L22 (= L17), E81 (= E76), I82 (= I77), S160 (= S155), D283 (= D281), K352 (= K351), R359 (= R358), R360 (= R359)
- binding magnesium ion: E264 (= E262), E276 (= E274)
1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
53% identity, 98% coverage: 8:386/387 of query aligns to 13:387/389 of 1eyzA
- active site: E114 (≠ D109), K154 (= K149), S159 (= S154), G161 (= G156), E264 (= E262), E276 (= E274), D283 (= D281), T284 (= T282), R360 (= R359)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R108), I152 (≠ V147), K154 (= K149), S159 (= S154), S160 (= S155), G161 (= G156), Q164 (= Q159), E192 (= E189), V195 (≠ I192), F197 (= F194), E200 (= E197), E264 (= E262), F266 (= F264), E276 (= E274)
- binding magnesium ion: E264 (= E262), E276 (= E274)
1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
53% identity, 98% coverage: 8:386/387 of query aligns to 13:384/386 of 1kjjA
- active site: E114 (≠ D109), K154 (= K149), S159 (= S154), G161 (= G156), E261 (= E262), E273 (= E274), D280 (= D281), T281 (= T282), R357 (= R359)
- binding phosphothiophosphoric acid-adenylate ester: R113 (= R108), I152 (≠ V147), K154 (= K149), S159 (= S154), S160 (= S155), G161 (= G156), Q164 (= Q159), E189 (= E189), V192 (≠ I192), E197 (= E197), Q219 (= Q220), E261 (= E262), F263 (= F264), E273 (= E274)
- binding magnesium ion: E261 (= E262), E273 (= E274)
1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
53% identity, 98% coverage: 8:386/387 of query aligns to 13:384/386 of 1kj8A
- active site: E114 (≠ D109), K154 (= K149), S159 (= S154), G161 (= G156), E261 (= E262), E273 (= E274), D280 (= D281), T281 (= T282), R357 (= R359)
- binding adenosine-5'-triphosphate: R113 (= R108), I152 (≠ V147), K154 (= K149), S159 (= S154), S160 (= S155), G161 (= G156), Q164 (= Q159), E189 (= E189), V192 (≠ I192), F194 (= F194), E197 (= E197), Q219 (= Q220), G222 (= G223), E261 (= E262), F263 (= F264), E273 (= E274)
- binding glycinamide ribonucleotide: G20 (= G15), E21 (= E16), L22 (= L17), E81 (= E76), I82 (= I77), S160 (= S155), D280 (= D281), K349 (= K351), R356 (= R358)
- binding magnesium ion: E261 (= E262), E273 (= E274)
1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
52% identity, 98% coverage: 8:386/387 of query aligns to 13:386/388 of 1kjqA
- active site: E114 (≠ D109), K154 (= K149), E263 (= E262), E275 (= E274), D282 (= D281), T283 (= T282), R359 (= R359)
- binding adenosine-5'-diphosphate: R113 (= R108), I152 (≠ V147), K154 (= K149), E191 (= E189), V193 (≠ F191), V194 (≠ I192), F196 (= F194), E199 (= E197), Q221 (= Q220), F265 (= F264), E275 (= E274)
- binding magnesium ion: E263 (= E262), E275 (= E274)
O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
49% identity, 98% coverage: 7:386/387 of query aligns to 17:410/430 of O58056
2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
47% identity, 98% coverage: 7:386/387 of query aligns to 19:399/409 of 2dwcB
- active site: E265 (= E262), E277 (= E274), D284 (= D281), T285 (= T282), R372 (= R359)
- binding adenosine-5'-diphosphate: R120 (= R108), H159 (≠ V147), K161 (= K149), H190 (≠ F191), I191 (= I192), F193 (= F194), E196 (= E197), F267 (= F264), E277 (= E274)
3ax6A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermotoga maritima
27% identity, 94% coverage: 23:387/387 of query aligns to 18:358/360 of 3ax6A
- active site: E231 (= E262), E244 (= E274), N251 (≠ D281), S252 (≠ T282), K330 (≠ R359)
- binding adenosine-5'-diphosphate: K101 (≠ R108), V136 (≠ M152), K138 (≠ S154), E164 (= E189), F166 (= F191), V167 (≠ I192), E172 (= E197), F233 (= F264), N243 (≠ S273)
3v4sA Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
28% identity, 94% coverage: 16:377/387 of query aligns to 17:365/380 of 3v4sA
- binding adenosine-5'-diphosphate: R106 (= R108), K146 (= K149), Y152 (vs. gap), G154 (= G156), Q157 (= Q159), W183 (≠ F191), V184 (≠ I192), E189 (= E197), N215 (≠ D224), F256 (= F264), N266 (≠ S273), E267 (= E274)
- binding carbonate ion: R271 (= R278), H273 (= H280), N274 (≠ D281)
3v4sB Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
28% identity, 94% coverage: 16:377/387 of query aligns to 19:367/381 of 3v4sB
- binding adenosine-5'-triphosphate: R108 (= R108), K148 (= K149), Y154 (vs. gap), D155 (≠ S155), G156 (= G156), Q159 (= Q159), E183 (= E189), W185 (≠ F191), V186 (≠ I192), F188 (= F194), E191 (= E197), H214 (≠ E221), N217 (≠ D224), E256 (= E262), F258 (= F264), E269 (= E274)
- binding carbonate ion: R273 (= R278), H275 (= H280), N276 (≠ D281)
- binding magnesium ion: T105 (= T105), E111 (≠ I111), E256 (= E262), E269 (= E274), L270 (= L275)
3r5hA Crystal structure of adp-air complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
28% identity, 94% coverage: 16:377/387 of query aligns to 18:366/383 of 3r5hA
- binding adenosine-5'-diphosphate: R107 (= R108), K147 (= K149), Q158 (= Q159), W184 (≠ F191), V185 (≠ I192), F187 (= F194), E190 (= E197), N216 (≠ D224), F257 (= F264), N267 (≠ S273), E268 (= E274)
- binding 5-aminoimidazole ribonucleotide: Q18 (≠ E16), L19 (= L17), E76 (= E76), Y153 (vs. gap), R272 (= R278), K340 (= K351), R347 (= R358)
Sites not aligning to the query:
3q2oB Crystal structure of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
28% identity, 94% coverage: 16:377/387 of query aligns to 18:366/377 of 3q2oB
4dlkA Crystal structure of atp-ca++ complex of purk: n5- carboxyaminoimidazole ribonucleotide synthetase (see paper)
28% identity, 94% coverage: 16:377/387 of query aligns to 18:366/380 of 4dlkA
- active site: Y153 (vs. gap), G155 (= G156), E255 (= E262), E268 (= E274), N275 (≠ D281), S276 (≠ T282), K348 (≠ R359)
- binding adenosine-5'-triphosphate: E76 (= E76), F77 (≠ I77), R107 (= R108), K147 (= K149), Y153 (vs. gap), D154 (≠ S155), G155 (= G156), Q158 (= Q159), W184 (≠ F191), V185 (≠ I192), F187 (= F194), E190 (= E197), E255 (= E262), F257 (= F264), N267 (≠ S273), E268 (= E274), R272 (= R278), H274 (= H280), N275 (≠ D281), K340 (= K351), R347 (= R358), K348 (≠ R359)
- binding calcium ion: E255 (= E262), E268 (= E274)
- binding phosphate ion: Q47 (= Q45), A49 (= A47)
5jqwA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with adp
27% identity, 77% coverage: 8:305/387 of query aligns to 2:290/365 of 5jqwA
- active site: Y144 (≠ S154), G146 (= G156), E247 (= E262), E259 (= E274), N266 (≠ D281), S267 (≠ T282)
- binding adenosine-5'-diphosphate: R98 (= R110), K138 (= K149), G143 (≠ S153), Y144 (≠ S154), D145 (≠ S155), G146 (= G156), V178 (≠ I192), E183 (= E197), H206 (≠ Q220), F249 (= F264), E259 (= E274)
Sites not aligning to the query:
3aw8A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermus thermophilus hb8
28% identity, 75% coverage: 72:361/387 of query aligns to 62:331/360 of 3aw8A
- active site: E240 (= E262), E252 (= E274), N259 (≠ D281), S260 (≠ T282), K329 (≠ R359)
- binding adenosine monophosphate: L135 (≠ M152), K137 (≠ S154), Q142 (= Q159), F168 (= F191), V169 (≠ I192), E174 (= E197), H197 (≠ Q220), F242 (= F264), E252 (= E274)
4ma0A The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with partially hydrolysed atp
27% identity, 77% coverage: 8:305/387 of query aligns to 2:290/366 of 4ma0A
- active site: Y144 (≠ S154), G146 (= G156), E247 (= E262), E259 (= E274), N266 (≠ D281), S267 (≠ T282)
- binding adenosine monophosphate: I136 (≠ V147), K138 (= K149), E175 (= E189), A176 (≠ E190), F177 (= F191), V178 (≠ I192), E183 (= E197), H206 (≠ Q220), F249 (= F264), E259 (= E274)
Sites not aligning to the query:
4mamA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with an adp analog, amp-cp
27% identity, 77% coverage: 8:305/387 of query aligns to 2:290/373 of 4mamA
- active site: Y144 (≠ S154), G146 (= G156), E247 (= E262), E259 (= E274), N266 (≠ D281), S267 (≠ T282)
- binding phosphomethylphosphonic acid adenosyl ester: R98 (= R110), I136 (≠ V147), K138 (= K149), Y144 (≠ S154), G146 (= G156), Q149 (= Q159), E175 (= E189), F177 (= F191), V178 (≠ I192), F180 (= F194), E183 (= E197), H206 (≠ Q220), F249 (= F264), E259 (= E274)
Sites not aligning to the query:
3k5hA Crystal structure of carboxyaminoimidazole ribonucleotide synthase from asperigillus clavatus complexed with atp (see paper)
28% identity, 90% coverage: 24:373/387 of query aligns to 22:367/382 of 3k5hA
- active site: Y152 (≠ S154), G154 (= G156), E254 (= E262), E267 (= E274), N274 (≠ D281), S275 (≠ T282), K353 (≠ R359)
- binding adenosine-5'-triphosphate: K104 (≠ R108), K146 (= K149), Y152 (≠ S154), D153 (≠ S155), G154 (= G156), N157 (≠ Q159), E181 (= E189), W183 (≠ F191), A184 (≠ I192), F186 (= F194), E189 (= E197), E254 (= E262), F256 (= F264), E267 (= E274)
- binding magnesium ion: E254 (= E262), E267 (= E274)
Query Sequence
>WP_012409334.1 NCBI__GCF_000020025.1:WP_012409334.1
MNIKLPQKLMLLGSGELGKEFAIAAQRLGNYVIAVDRYANAPAMQVADASEVISMLSADD
LEAVVKKHQPNIIIPEIEAIRTEKLVEFEQRGITVIPTAAATNYTMNRDRIRELAHKQLG
IRTATYGYATTLEELITISEEIGFSNVVKPVMSSSGKGQSVVQDKSEVEKAWNYAIANSR
GDSQKVIVEEFINFEIEITLLTIKQWDAPTIFCSPIGHRQERGDYQESWQPVLISEDKIL
KSQEIAIKVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLR
AILGLPIPHIEQLGASASAVILASEKSDSIGFTGVADALSEKDVDIRLFGKPNAHPYRRM
GVALAKGQNIEEAREKATKAASKITII
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory