SitesBLAST
Comparing WP_012409829.1 NCBI__GCF_000020025.1:WP_012409829.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6kfuA A acp-amt fusion protein of hybrid polyketide/non-ribosomal peptide synthetase
51% identity, 93% coverage: 9:461/485 of query aligns to 83:527/527 of 6kfuA
- active site: Y240 (= Y165), D334 (= D259), K362 (= K287)
- binding pyridoxal-5'-phosphate: G213 (= G138), T214 (= T139), Y240 (= Y165), H241 (= H166), G242 (= G167), E302 (= E227), D334 (= D259), I336 (≠ V261), I337 (= I262), K362 (= K287)
6kfrA Amino-transferase (amt) domain - arg complex of hybrid polyketide/non- ribosomal peptide synthetase
51% identity, 93% coverage: 9:461/485 of query aligns to 5:449/449 of 6kfrA
- active site: R38 (≠ M42), Y162 (= Y165), D256 (= D259), K284 (= K287)
- binding arginine: Y162 (= Y165), A328 (= A329), G329 (= G330), T330 (= T331), W413 (≠ G426)
- binding pyridoxal-5'-phosphate: G135 (= G138), T136 (= T139), Y162 (= Y165), H163 (= H166), D256 (= D259), I258 (≠ V261), I259 (= I262), K284 (= K287), G329 (= G330), T330 (= T331)
P23893 Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 from Escherichia coli (strain K12) (see paper)
35% identity, 81% coverage: 57:449/485 of query aligns to 33:420/426 of P23893
- K265 (= K287) mutation to R: 2% of wild-type activity.
3bs8A Crystal structure of glutamate 1-semialdehyde aminotransferase complexed with pyridoxamine-5'-phosphate from bacillus subtilis (see paper)
35% identity, 83% coverage: 57:457/485 of query aligns to 36:429/430 of 3bs8A
- active site: Y145 (= Y165), E207 (= E227), D240 (= D259), M243 (≠ I262), K268 (= K287), G401 (= G429)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G138), T119 (= T139), Y145 (= Y165), H146 (= H166), E207 (= E227), N212 (≠ R232), D240 (= D259), V242 (= V261), K268 (= K287)
Sites not aligning to the query:
6w80A Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from stenotrophomonas maltophilia k279a in complex with plp
36% identity, 79% coverage: 57:441/485 of query aligns to 40:415/430 of 6w80A
- active site: Y149 (= Y165), D241 (= D259), K269 (= K287)
- binding pyridoxal-5'-phosphate: S121 (≠ T137), G122 (= G138), T123 (= T139), Y149 (= Y165), H150 (= H166), E208 (= E227), N213 (≠ R232), D241 (= D259), V243 (= V261), K269 (= K287)
Sites not aligning to the query:
P42799 Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic; AtGSA1; GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 81% coverage: 57:447/485 of query aligns to 82:466/474 of P42799
- K314 (= K287) modified: N6-(pyridoxal phosphate)lysine
Q42522 Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic; GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 82% coverage: 57:455/485 of query aligns to 80:472/472 of Q42522
- R92 (≠ W69) mutation to K: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with S-162.
- G162 (= G138) mutation to S: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with K-92.
5hdmB Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
34% identity, 81% coverage: 57:447/485 of query aligns to 36:420/428 of 5hdmB
- active site: Y145 (= Y165), E207 (= E227), D240 (= D259), M243 (≠ I262), K268 (= K287), A402 (≠ G429)
- binding pyridoxal-5'-phosphate: G118 (= G138), T119 (= T139), Y145 (= Y165), E207 (= E227), N212 (≠ R232), D240 (= D259), V242 (= V261), M243 (≠ I262), K268 (= K287)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G138), T119 (= T139), Y145 (= Y165), E207 (= E227), N212 (≠ R232), D240 (= D259), V242 (= V261), M243 (≠ I262), K268 (= K287)
Sites not aligning to the query:
5hdmA Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
34% identity, 81% coverage: 57:447/485 of query aligns to 36:420/428 of 5hdmA
- active site: Y145 (= Y165), E207 (= E227), D240 (= D259), M243 (≠ I262), K268 (= K287), A402 (≠ G429)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G138), T119 (= T139), Y145 (= Y165), G147 (= G167), E207 (= E227), N212 (≠ R232), D240 (= D259), V242 (= V261), K268 (= K287)
Sites not aligning to the query:
2e7uA Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from thermus thermophilus hb8
33% identity, 81% coverage: 62:452/485 of query aligns to 40:424/424 of 2e7uA
- active site: Y144 (= Y165), E206 (= E227), D238 (= D259), M241 (≠ I262), K266 (= K287), A401 (≠ G429)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G138), T118 (= T139), Y144 (= Y165), H145 (= H166), N211 (≠ R232), D238 (= D259), V240 (= V261)
Sites not aligning to the query:
5i92F Crystal structure of glutamate-1-semialdehyde 2,1- aminomutase (gsa) from pseudomonas aeruginosa
33% identity, 81% coverage: 57:447/485 of query aligns to 33:413/420 of 5i92F
Sites not aligning to the query:
3fqaA Gabaculien complex of gabaculine resistant gsam version (see paper)
35% identity, 82% coverage: 57:455/485 of query aligns to 34:426/426 of 3fqaA
- active site: Y143 (= Y165), D238 (= D259), I241 (= I262), K266 (= K287), A400 (≠ G429)
- binding 3-aminobenzoic acid: W60 (≠ L83)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G116 (= G138), T117 (= T139), Y143 (= Y165), E205 (= E227), N210 (≠ R232), D238 (= D259), V240 (= V261), I241 (= I262)
Sites not aligning to the query:
2gsaB Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
34% identity, 82% coverage: 57:455/485 of query aligns to 35:427/427 of 2gsaB
- active site: Y144 (= Y165), E206 (= E227), D239 (= D259), M242 (≠ I262), K267 (= K287), A401 (≠ G429)
- binding pyridoxal-5'-phosphate: G117 (= G138), T118 (= T139), Y144 (= Y165), E206 (= E227), N211 (≠ R232), D239 (= D259), V241 (= V261), M242 (≠ I262), K267 (= K287)
Sites not aligning to the query:
2gsaA Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
34% identity, 82% coverage: 57:455/485 of query aligns to 35:427/427 of 2gsaA
- active site: Y144 (= Y165), E206 (= E227), D239 (= D259), M242 (≠ I262), K267 (= K287), A401 (≠ G429)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G138), T118 (= T139), Y144 (= Y165), H145 (= H166), G146 (= G167), N211 (≠ R232), D239 (= D259), V241 (= V261), K267 (= K287)
Sites not aligning to the query:
3usfA Crystal structure of dava-4
34% identity, 82% coverage: 57:455/485 of query aligns to 35:427/427 of 3usfA
- active site: Y144 (= Y165), D239 (= D259), M242 (≠ I262), K267 (= K287), A401 (≠ G429)
- binding (4s)-4,5-diaminopentanoic acid: S157 (≠ T178), K267 (= K287), E400 (vs. gap)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G138), T118 (= T139), Y144 (= Y165), N211 (≠ R232), D239 (= D259), V241 (= V261), K267 (= K287)
Sites not aligning to the query:
3fq7A Gabaculine complex of gsam (see paper)
34% identity, 82% coverage: 57:455/485 of query aligns to 35:427/427 of 3fq7A
- active site: Y144 (= Y165), D239 (= D259), M242 (≠ I262), K267 (= K287), A401 (≠ G429)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: W61 (≠ L83), G117 (= G138), T118 (= T139), Y144 (= Y165), H145 (= H166), E206 (= E227), N211 (≠ R232), D239 (= D259), V241 (= V261), M242 (≠ I262), K267 (= K287), G298 (= G330), T299 (= T331), E400 (vs. gap)
Sites not aligning to the query:
2hp2A Inter-subunit signaling in gsam (see paper)
34% identity, 82% coverage: 57:455/485 of query aligns to 35:427/427 of 2hp2A
- active site: Y144 (= Y165), D239 (= D259), M242 (≠ I262), K267 (= K287), A401 (≠ G429)
- binding (4s)-4,5-diaminopentanoic acid: G298 (= G330), T299 (= T331)
- binding (4r)-5-amino-4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: G117 (= G138), T118 (= T139), Y144 (= Y165), H145 (= H166), E206 (= E227), N211 (≠ R232), D239 (= D259), V241 (= V261), M242 (≠ I262), K267 (= K287)
- binding pyridoxal-5'-phosphate: G298 (= G330), T299 (= T331)
Sites not aligning to the query:
2hp1A Inter-subunit signaling in gsam (see paper)
34% identity, 82% coverage: 57:455/485 of query aligns to 35:427/427 of 2hp1A
- active site: Y144 (= Y165), D239 (= D259), M242 (≠ I262), K267 (= K287), A401 (≠ G429)
- binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: W61 (≠ L83), S116 (≠ T137), G117 (= G138), T118 (= T139), Y144 (= Y165), H145 (= H166), G146 (= G167), E206 (= E227), N211 (≠ R232), D239 (= D259), V241 (= V261), M242 (≠ I262), K267 (= K287), E400 (vs. gap)
Sites not aligning to the query:
2hozA Inter-subunit signaling in gsam (see paper)
34% identity, 82% coverage: 57:455/485 of query aligns to 35:427/427 of 2hozA
- active site: Y144 (= Y165), D239 (= D259), M242 (≠ I262), K267 (= K287), A401 (≠ G429)
- binding (4s)-4,5-diaminopentanoic acid: E141 (≠ S162), G156 (≠ E177), S157 (≠ T178), P182 (≠ D204), N368 (≠ L401), E370 (= E403), K373 (≠ T406)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G138), T118 (= T139), Y144 (= Y165), H145 (= H166), G146 (= G167), E206 (= E227), N211 (≠ R232), D239 (= D259), G298 (= G330), T299 (= T331)
Sites not aligning to the query:
3k28A Crystal structure of a glutamate-1-semialdehyde aminotransferase from bacillus anthracis with bound pyridoxal 5'phosphate
34% identity, 79% coverage: 57:440/485 of query aligns to 36:407/423 of 3k28A
- active site: Y145 (= Y165), E202 (= E227), D235 (= D259), M238 (≠ I262), K263 (= K287), G396 (= G429)
- binding calcium ion: I103 (≠ C124), V106 (≠ L126), P107 (≠ T127), I109 (≠ M129)
- binding pyridoxal-5'-phosphate: G118 (= G138), T119 (= T139), Y145 (= Y165), H146 (= H166), G147 (= G167), E202 (= E227), D235 (= D259), V237 (= V261), M238 (≠ I262), K263 (= K287)
Sites not aligning to the query:
Query Sequence
>WP_012409829.1 NCBI__GCF_000020025.1:WP_012409829.1
MNKAGNHLQQRYLEAFTTRYNERTKKSKQLAQTYRPIITSNMGPVGFSLSCKEMCYPITA
KRSLGSRIWDVDGNEYIDFMMGLGVNLFGHNPPFIQAALKEQLEKGIQIGPQSELTGEVG
ELICELTGMERVAFSNTGTEAVMAAIRLARTATGRNKIVLFSGSYHGHFDGTLVEVETID
GIPHTVPITSGIPQNIVEDYLVLDYGNPQSLDAIRANKHELAAVLVEPVQSRRPDLQPKD
FLLQLRQLTKELGIALIFDEVITGFRVHPGGAQAWFGVEADIATYGKIVGGGMPIGIIAG
KATYMDRIDGGMWNYGDASQPQVETTYFAGTFRRHPLAMAAALAVLKHLKMQGSQLQQQL
NQRMSQLVETLNAYFEEEEVPLRLRNFSSWFSPAFLENFSLLEYATPSELRELLYYHLLD
KGVMLGGPGGYLSTAHTDEDINYIIQAFKDSVRELRKGGFLPPIAPQNLVKEFSLETREQ
IADVR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory