SitesBLAST
Comparing WP_012410441.1 NCBI__GCF_000020025.1:WP_012410441.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2gsaB Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
85% identity, 99% coverage: 7:432/432 of query aligns to 2:427/427 of 2gsaB
- active site: V21 (= V26), Y144 (= Y149), E206 (= E211), D239 (= D244), M242 (= M247), K267 (= K272), A401 (= A406)
- binding pyridoxal-5'-phosphate: G117 (= G122), T118 (= T123), Y144 (= Y149), E206 (= E211), N211 (= N216), D239 (= D244), V241 (= V246), M242 (= M247), K267 (= K272)
2gsaA Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
85% identity, 99% coverage: 7:432/432 of query aligns to 2:427/427 of 2gsaA
- active site: V21 (= V26), Y144 (= Y149), E206 (= E211), D239 (= D244), M242 (= M247), K267 (= K272), A401 (= A406)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G122), T118 (= T123), Y144 (= Y149), H145 (= H150), G146 (= G151), N211 (= N216), D239 (= D244), V241 (= V246), K267 (= K272)
3usfA Crystal structure of dava-4
84% identity, 99% coverage: 7:432/432 of query aligns to 2:427/427 of 3usfA
- active site: V21 (= V26), Y144 (= Y149), D239 (= D244), M242 (= M247), K267 (= K272), A401 (= A406)
- binding (4s)-4,5-diaminopentanoic acid: S23 (= S28), V25 (= V30), S157 (= S162), K267 (= K272), E400 (= E405)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G122), T118 (= T123), Y144 (= Y149), N211 (= N216), D239 (= D244), V241 (= V246), K267 (= K272)
3fq7A Gabaculine complex of gsam (see paper)
84% identity, 99% coverage: 7:432/432 of query aligns to 2:427/427 of 3fq7A
- active site: V21 (= V26), Y144 (= Y149), D239 (= D244), M242 (= M247), K267 (= K272), A401 (= A406)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: S23 (= S28), V25 (= V30), W61 (= W66), G117 (= G122), T118 (= T123), Y144 (= Y149), H145 (= H150), E206 (= E211), N211 (= N216), D239 (= D244), V241 (= V246), M242 (= M247), K267 (= K272), G298 (= G303), T299 (= T304), E400 (= E405)
2hp2A Inter-subunit signaling in gsam (see paper)
84% identity, 99% coverage: 7:432/432 of query aligns to 2:427/427 of 2hp2A
- active site: V21 (= V26), Y144 (= Y149), D239 (= D244), M242 (= M247), K267 (= K272), A401 (= A406)
- binding (4s)-4,5-diaminopentanoic acid: G298 (= G303), T299 (= T304)
- binding (4r)-5-amino-4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S28), G117 (= G122), T118 (= T123), Y144 (= Y149), H145 (= H150), E206 (= E211), N211 (= N216), D239 (= D244), V241 (= V246), M242 (= M247), K267 (= K272)
- binding pyridoxal-5'-phosphate: G298 (= G303), T299 (= T304)
2hp1A Inter-subunit signaling in gsam (see paper)
84% identity, 99% coverage: 7:432/432 of query aligns to 2:427/427 of 2hp1A
- active site: V21 (= V26), Y144 (= Y149), D239 (= D244), M242 (= M247), K267 (= K272), A401 (= A406)
- binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S28), V25 (= V30), W61 (= W66), S116 (= S121), G117 (= G122), T118 (= T123), Y144 (= Y149), H145 (= H150), G146 (= G151), E206 (= E211), N211 (= N216), D239 (= D244), V241 (= V246), M242 (= M247), K267 (= K272), E400 (= E405)
2hozA Inter-subunit signaling in gsam (see paper)
84% identity, 99% coverage: 7:432/432 of query aligns to 2:427/427 of 2hozA
- active site: V21 (= V26), Y144 (= Y149), D239 (= D244), M242 (= M247), K267 (= K272), A401 (= A406)
- binding (4s)-4,5-diaminopentanoic acid: E141 (= E146), G156 (= G161), S157 (= S162), P182 (= P187), N368 (= N373), E370 (= E375), K373 (= K378)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G122), T118 (= T123), Y144 (= Y149), H145 (= H150), G146 (= G151), E206 (= E211), N211 (= N216), D239 (= D244), G298 (= G303), T299 (= T304)
3fqaA Gabaculien complex of gabaculine resistant gsam version (see paper)
84% identity, 99% coverage: 7:432/432 of query aligns to 1:426/426 of 3fqaA
- active site: V20 (= V26), Y143 (= Y149), D238 (= D244), I241 (≠ M247), K266 (= K272), A400 (= A406)
- binding 3-aminobenzoic acid: S22 (= S28), R25 (= R31), W60 (= W66)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G116 (= G122), T117 (= T123), Y143 (= Y149), E205 (= E211), N210 (= N216), D238 (= D244), V240 (= V246), I241 (≠ M247)
3usfB Crystal structure of dava-4
79% identity, 99% coverage: 7:432/432 of query aligns to 2:402/402 of 3usfB
- active site: V21 (= V26), Y144 (= Y149), E181 (= E211), D214 (= D244), M217 (= M247), K242 (= K272), A376 (= A406)
- binding (4s)-4,5-diaminopentanoic acid: G273 (= G303), T274 (= T304)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G122), T118 (= T123), Y144 (= Y149), E181 (= E211), N186 (= N216), D214 (= D244), V216 (= V246), M217 (= M247), K242 (= K272)
2hp1B Inter-subunit signaling in gsam (see paper)
79% identity, 99% coverage: 7:432/432 of query aligns to 2:398/398 of 2hp1B
- active site: V21 (= V26), Y144 (= Y149), E177 (= E211), D210 (= D244), M213 (= M247), K238 (= K272), A372 (= A406)
- binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: G269 (= G303), T270 (= T304)
- binding (4s)-4,5-diaminopentanoic acid: S23 (= S28), V25 (= V30), R26 (= R31), W61 (= W66), Y144 (= Y149)
- binding pyridoxal-5'-phosphate: S116 (= S121), G117 (= G122), T118 (= T123), Y144 (= Y149), E177 (= E211), N182 (= N216), D210 (= D244), V212 (= V246), M213 (= M247), K238 (= K272)
Q42522 Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic; GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
76% identity, 98% coverage: 10:432/432 of query aligns to 50:472/472 of Q42522
- R92 (≠ W52) mutation to K: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with S-162.
- G162 (= G122) mutation to S: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with K-92.
P42799 Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic; AtGSA1; GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
75% identity, 98% coverage: 10:432/432 of query aligns to 52:474/474 of P42799
- K314 (= K272) modified: N6-(pyridoxal phosphate)lysine
5hdmB Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
75% identity, 98% coverage: 10:432/432 of query aligns to 6:428/428 of 5hdmB
- active site: V22 (= V26), Y145 (= Y149), E207 (= E211), D240 (= D244), M243 (= M247), K268 (= K272), A402 (= A406)
- binding pyridoxal-5'-phosphate: G118 (= G122), T119 (= T123), Y145 (= Y149), E207 (= E211), N212 (= N216), D240 (= D244), V242 (= V246), M243 (= M247), K268 (= K272)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G122), T119 (= T123), Y145 (= Y149), E207 (= E211), N212 (= N216), D240 (= D244), V242 (= V246), M243 (= M247), K268 (= K272)
5hdmA Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
75% identity, 98% coverage: 10:432/432 of query aligns to 6:428/428 of 5hdmA
- active site: V22 (= V26), Y145 (= Y149), E207 (= E211), D240 (= D244), M243 (= M247), K268 (= K272), A402 (= A406)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G122), T119 (= T123), Y145 (= Y149), G147 (= G151), E207 (= E211), N212 (= N216), D240 (= D244), V242 (= V246), K268 (= K272)
2cfbA Glutamate-1-semialdehyde 2,1-aminomutase from thermosynechococcus elongatus (see paper)
74% identity, 91% coverage: 40:432/432 of query aligns to 1:360/360 of 2cfbA
- active site: Y110 (= Y149), E144 (= E211), D177 (= D244), M180 (= M247), K205 (= K272), A334 (= A406)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G83 (= G122), T84 (= T123), Y110 (= Y149), E144 (= E211), D177 (= D244), V179 (= V246), M180 (= M247), K205 (= K272)
3bs8A Crystal structure of glutamate 1-semialdehyde aminotransferase complexed with pyridoxamine-5'-phosphate from bacillus subtilis (see paper)
58% identity, 98% coverage: 10:432/432 of query aligns to 6:427/430 of 3bs8A
- active site: V22 (= V26), Y145 (= Y149), E207 (= E211), D240 (= D244), M243 (= M247), K268 (= K272), G401 (≠ A406)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G122), T119 (= T123), Y145 (= Y149), H146 (= H150), E207 (= E211), N212 (= N216), D240 (= D244), V242 (= V246), K268 (= K272)
P23893 Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 from Escherichia coli (strain K12) (see paper)
56% identity, 98% coverage: 9:432/432 of query aligns to 2:426/426 of P23893
- K265 (= K272) mutation to R: 2% of wild-type activity.
6w80A Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from stenotrophomonas maltophilia k279a in complex with plp
58% identity, 96% coverage: 10:422/432 of query aligns to 10:419/430 of 6w80A
- active site: V26 (= V26), Y149 (= Y149), D241 (= D244), K269 (= K272)
- binding pyridoxal-5'-phosphate: S121 (= S121), G122 (= G122), T123 (= T123), Y149 (= Y149), H150 (= H150), E208 (= E211), N213 (= N216), D241 (= D244), V243 (= V246), K269 (= K272)
3k28A Crystal structure of a glutamate-1-semialdehyde aminotransferase from bacillus anthracis with bound pyridoxal 5'phosphate
58% identity, 99% coverage: 7:432/432 of query aligns to 3:422/423 of 3k28A
- active site: V22 (= V26), Y145 (= Y149), E202 (= E211), D235 (= D244), M238 (= M247), K263 (= K272), G396 (≠ A406)
- binding calcium ion: I103 (= I107), V106 (= V110), P107 (= P111), I109 (= I113)
- binding pyridoxal-5'-phosphate: G118 (= G122), T119 (= T123), Y145 (= Y149), H146 (= H150), G147 (= G151), E202 (= E211), D235 (= D244), V237 (= V246), M238 (= M247), K263 (= K272)
2e7uA Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from thermus thermophilus hb8
55% identity, 97% coverage: 11:429/432 of query aligns to 6:424/424 of 2e7uA
- active site: V21 (= V26), Y144 (= Y149), E206 (= E211), D238 (= D244), M241 (= M247), K266 (= K272), A401 (= A406)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G122), T118 (= T123), Y144 (= Y149), H145 (= H150), N211 (= N216), D238 (= D244), V240 (= V246)
Query Sequence
>WP_012410441.1 NCBI__GCF_000020025.1:WP_012410441.1
MVNTTIKTTKSQEVFAAAQNLMPGGVSSPVRAFKSVGGQPIVFDRVKGAYIWDVDGNQYI
DYVGTWGPAICGHAHPEVIAALHEALEKGTSFGAPSTLENVLAEMVIDAVPSIEMVRFVN
SGTEACMGVLRLMRAFTNRDKIIKFEGCYHGHADTFLVKAGSGVATLGLPDSPGVPKAAT
STTLTAPYNDLESVKALFEENRDEIAGVILEPVVGNAGFIAPDAGFLEGLRELTHEYGAL
LVFDEVMTGFRIAYGGAQEKFGVTPDLTTLGKVIGGGLPVGAYGGRRDIMSMVAPAGPVY
QAGTLSGNPLAMTAGIKTLELLQKPGTYDYLERITKKLADGLLQIAKETGHTACGGQISA
MFGLFFTSGPVHNYEDAKKSDTAKFGRFHRGMLERGVYLAPSQFEAGFTSFAHTEEDIDQ
TLAIARDVMSSL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory