SitesBLAST

Q56YA5 Serine--glyoxylate aminotransferase; Alanine--glyoxylate aminotransferase; AGT; Asparagine aminotransferase; Serine--pyruvate aminotransferase; EC 2.6.1.45; EC 2.6.1.44; EC 2.6.1.-; EC 2.6.1.51 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
36% identity, 97% coverage: 7:377/384 of query aligns to 12:386/401 of Q56YA5

query
sites
Q56YA5

L

L

M

F

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P

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G

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N

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x
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T

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G

A

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TGT 68:70 (≠ SGT 62:64) binding pyridoxal 5'-phosphate
T148 (= T142) binding pyridoxal 5'-phosphate
QK 200:201 (= QK 193:194) binding pyridoxal 5'-phosphate
K201 (= K194) binding 3-hydroxypyruvate
P251 (= P243) mutation to L: Abolishes aminotransferase activity.
R347 (= R337) binding 3-hydroxypyruvate

6pk1A Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana in presence of serine (see paper)
36% identity, 97% coverage: 7:377/384 of query aligns to 10:384/399 of 6pk1A

query
sites
6pk1A

L

L

M

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T

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A

binding 3-hydroxypyruvic acid: F93 (= F89), R345 (= R337)
binding pyridoxal-5'-phosphate: T66 (≠ S62), G67 (= G63), T68 (= T64), W71 (≠ V67), F93 (= F89), T146 (= T142), D173 (= D168), V175 (= V170), K199 (= K194)

2dr1A Crystal structure of the ph1308 protein from pyrococcus horikoshii ot3
34% identity, 94% coverage: 4:363/384 of query aligns to 16:375/381 of 2dr1A

query
sites
2dr1A

K

K

L

Y

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K

L

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M

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binding pyridoxal-5'-phosphate: S75 (= S62), G76 (= G63), T77 (= T64), F102 (= F89), T153 (= T142), D178 (= D168), V180 (= V170), S201 (≠ G191), K204 (= K194)

1vjoA Crystal structure of alanine--glyoxylate aminotransferase (alr1004) from nostoc sp. At 1.70 a resolution (see paper)
33% identity, 92% coverage: 6:360/384 of query aligns to 20:376/377 of 1vjoA

query
sites
1vjoA

M

L

L

L

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L

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A

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G

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T

G
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G

-
x
T

-

A

A

A

V

M

E

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A

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G

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I

I

I

A

N

N

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P

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G

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D

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N

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F
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F

G

G

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S

A

E

E

T
|
T

S

S

T

T

G

G

V

A

L

R

N

Q

D

P

L

L

E

E

S

G

I

V

N

G

R

E

H

L

V

C

K

R

A

E

H

F

G

G

E

-

A

T

L

L

I

L

V

V

D
|
D

A

T

V
|
V

T

T

S

S

L

L

G

G

A

G

F

V

N

P

L

I

P

F

V

L

D

D

A

A

W

W

G

G

L

V

D

D

I

L

V

A

A

Y

S

S

G

C

S

S

Q

Q

K
|
K

G

G

Y

L

M

G

I

C

P

S

P

P

G

G

L

A

G

S

F

P

V

F

S

T

V

M

S

S

P

S

K

R

A

A

W

I

E

E

A

K

Y

L

-

Q

-

R

K

R

T

R

A

T

K

K

L

V

P

A

K

N

Y

W

Y

Y

L

L

D

D

-

M

-

N

-

L

L

L

G

G

K

K

Y

Y

R

-

K

-

A

W

T

G

A

S

K

E

N

R

T

V

T

Y

P

H

F

H

T

T

P

A

P

P

V

I

N

N

L

L

I

Y

V

Y

A

A

L

L

H

R

T

E

T

A

L

L

R

R

I

L

M

I

K

A

E

Q

E

E

G

G

L

L

E

A

S

N

I

C

F

W

A

Q

R

R

H

H

E

Q

R

K

L

N

K

V

N

E

A

Y

T

L

R

W

A

E

A

R

I

L

Q

E

G

D

L

I

N

G

L

L

P

S

L

L

F

H

A

V

A

E

D

K

S

E

S

Y

A

R

S

L

P

P

A

T

I

L

T

T

A

T

V

V

A

C

-

I

P

P

Q

D

G

G

I

V

E

D

S

G

D

K

K

A

I

V

R

A

S

R

L

R

M

L

K

L

K

N

R

E

F

H

D

N

I

I

A

E

L

V

A

G

G

G

Q

L

D

G

H

E

L

L

S

A

N

G

K

K

I

V

F

W

R

R

I

V

G

G

H

L

L

M

G

G

F

F

V

N

S

S

D

R

R

K

D

E

-

S

I

V

L

D

S

Q

C

L

I

I

A

P

S

A

L

L

E

E

V

Q

T

V

L

L

binding pyridoxal-5'-phosphate: T77 (= T64), G78 (= G65), T79 (vs. gap), F104 (= F89), T154 (= T142), D179 (= D168), V181 (= V170), K205 (= K194)

1iugA The crystal structure of aspartate aminotransferase which belongs to subgroup iv from thermus thermophilus (see paper)
35% identity, 93% coverage: 7:363/384 of query aligns to 3:347/348 of 1iugA

query
sites
1iugA

L

L

M

L

I

T

P

P

G

G

P

P

T

V

P

R

V

L

P

H

E

P

A

K

A

A

L

L

L

E

A

A

L

L

A

A

K

R

H

P

P

Q

I

L

G

H

H

H

R

R

T

T

S

E

E

A

F

A

S

R

N

E

I

V

L

F

A

L

E

K

V

A

T

R

E

G

N

L

L

L

K

R

W

E

L

A

H

F

Q

R

T

T

Q

E

T

G

D

E

V

V

L

L

T

I

L

L

N

T

V

G

S

S

G

G

T

T

G

L

A

A

V

M

E

E

A

A

G

L

I

V

I

K

N

N

F

L

L

F

S

A

P

P

G

G

D

E

R

R

I

V

L

L

V

V

G

P

S

V

N

Y

G

G

K
|
K

F

F

G

S

E

E

R

R

W

F

V

Y

E

E

V

I

G

A

Q

L

A

E

Y

A

G

G

L

L

N

V

V

V

E

E

E

R

V

L

K

D

V

Y

E

P

W

Y

G

G

K

D

P

T

L

P

D

R

P

P

A

E

V

D

F

V

A

A

E

K

K

E

L

G

Q

Y

A

A

D

-

T

-

Q

-

K

-

Q

-

I

-

K

-

A

G

V

L

I

L

T

V

H

H

S

S

E

E

T

T

S

S

T

T

G

G

V

A

L

L

N

A

D

D

L

L

E

P

S

A

I

L

N

A

R

R

H

A

V

F

K

K

A

E

H

K

G

N

-

P

E

E

A

G

L

L

I

V

I

G

V

A

D

D

A

M

V

V

T

T

S

S

L

L

G

L

A

V

F

G

N

E

L

V

P

A

V

L

D

E

A

A

W

M

G

G

L

V

D

D

I

A

V

A

A

A

S

S

G

G

S

S

Q

Q

K
|
K

G

G

Y

L

M

M

I

C

P

P

G

G

L

L

G

G

F

F

V

V

S

A

V

L

S

S

P

P

K

R

A

A

W

L

E

E

A

R

Y

L

K

K

T

-

A

-

K

-

L

-

P

P

K

R

-

G

Y

Y

L

L

D

D

L

L

G

A

K

R

Y

E

R

L

K

K

A

A

T

Q

A

K

K

E

N

G

T

E

T

S

P

A

F

W

T

T

P

P

P

A

V

I

N

N

L

L

I

V

V

L

A

A

L

V

H

A

T

A

T

V

L

L

R

-

I

-

M

-

K

-

E

E

G

V

L

L

E

P

S

R

I

L

F

-

A

E

R

E

H

H

E

L

R

A

L

L

K

K

N

A

A

W

T

Q

R

N

A

A

A

L

I

L

Q

Y

G

G

L

V

N

G

L

E

P

E

-

G

L

L

F

R

A

P

A

V

D

P

S

K

S

R

A

F

S

S

P

P

A

A

I

V

T

A

A

A

V

F

A

Y

-

L

P

P

Q

E

G

G

I

V

E

P

S

Y

D

A

K

R

I

V

R

K

S

E

L

A

M

F

K

A

K

Q

R

R

F

G

D

A

I

V

A

-

L

I

A

A

G

G

Q

Q

D

G

H

P

L

L

S

K

N

G

K

K

I

V

F

F

R

R

I

L

G

S

H

L

L

M

G

G

F

A

V

Y

S

D

D

R

R

Y

D

E

I

A

L

L

S

G

C

V

I

A

A

G

S

M

L

F

E

R

V

E

T

V

L

L

T

E

E

E

L

I

binding phosphate ion: K84 (= K88), K184 (= K194)

3islA Crystal structure of ureidoglycine-glyoxylate aminotransferase (pucg) from bacillus subtilis
29% identity, 98% coverage: 7:383/384 of query aligns to 10:387/387 of 3islA

query
sites
3islA

L

I

M

M

I

T

P

P

G

G

P

P

T

V

P

E

V

V

P

D

E

P

A

R

A

V

L

L

L

R

A

V

L

M

A

S

K

T

H

P

P

V

I

V

G

G

H

Q

R

F

T

D

S

P

E

A

F

F

S

T

N

G

I

I

L

M

A

N

E

E

V

T

T

M

E

E

N

M

L

L

K

R

W

E

L

L

H

F

Q

Q

T

T

Q

K

T

N

D

R

-

W

V

A

L

Y

T

P

L

I

N

D

V

G

S
x
T

G
x
S

T
x
R

G

A

A

G

V

I

E

E

A

A

G

V

I

L

I

A

N

S

F

V

L

I

S

E

P

P

G

E

D

D

R

D

I

V

L

L

V

I

G

P

S

I

N

Y

G

G

K

R

F
|
F

G

G

E

Y

R

L

W

L

V

T

E

E

V

I

G

A

Q

E

A

R

Y

Y

G

G

L

A

N

N

V

V

E

H

E

M

V

L

K

E

V

C

E

E

W

W

G

G

K

T

P

V

L

F

D

D

P

P

A

E

V

D

F

I

A

I

E

R

K

E

L

I

Q

K

A

-

D

-

T

-

Q

K

K

V

Q

K

I

P

K

K

A

I

V

V

I

A

I

M

T

V

H

H

S

G

E

E

T
|
T

S

S

T

T

G

G

V

R

L

I

N

H

D

P

L

L

E

K

S

A

I

I

N

G

R

E

H

A

V

C

K

R

A

T

H

E

G

-

E

D

A

A

L

L

I

F

I

I

V

V

D
|
D

A

A

V
|
V

T

A

S

T

L

I

G

G

A

G

F

C

N

E

L

V

P

K

V

V

D

D

A

E

W

W

G

K

L

I

D

D

I

A

V

A

A

I

S

G

G

G

S

T

Q
|
Q

K
|
K

G

C

Y

L

M

S

I

V

P

P

P

S

G

G

L

M

G

A

F

P

V

I

S

T

V

Y

S

N

P

E

K

R

A

V

W

A

E

D

-

V

-

I

A

A

Y

A

K

R

T

N

A

R

K

P

L

I

P

T

K

S

Y

N

Y

Y

L

F

D

D

L

L

G

S

K

Q

Y

L

R

E

K

D

A

Y

-

W

T

S

A

E

K

R

N

R

T

L

T

N

P

H

F

H

T

T

P

E

P

A

V

T

N

T

L

M

I

L

V

Y

A

A

L

L

H

R

T

E

T

G

L

V

R

R

I

L

M

V

K

L

E

E

G

G

L

L

E

E

S

T

I

R

F

F

A

E

R

R

H

H

E

R

R

H

L

H

K

E

N

A

A

A

T

L

R

A

A

A

A

G

I

I

Q

K

G

A

L

M

N

G

L

L

P

R

L

L

F

F

A

G

A

D

D

D

S

S

S

C

A

K

S

M

P

P

A

V

I

V

T

T

A

C

V

V

A

E

-

I

P

P

Q

G

G

G

I

I

E

D

S

G

D

E

K

S

I

V

R

R

S

D

L

M

M

L

K

L

K

A

R

Q

F

F

D

G

I

I

A

E

L

I

A

A

G

S

Q

F

D

G

H

P

L

L

S

A

N

G

K

K

I

I

F

W

R

R

I

I

G

G

H

T

L

M

G

G

F

Y

V

S

S

C

D

R

R

K

D

E

-

N

I

V

L

L

S

F

C

V

I

L

A

A

S

G

L

L

E

E

V

A

T

V

L

L

T

L

E

R

-

H

-

N

L

A

G

G

Y

I

E

E

D

-

F

-

T

-

P

A

G

G

S

K

G

A

I

L

A

Q

A

A

V

L

K

D

V

V

F

Y

S

E

Q

N

binding pyridoxal-5'-phosphate: T66 (≠ S62), S67 (≠ G63), R68 (≠ T64), F93 (= F89), T143 (= T142), D168 (= D168), V170 (= V170), Q193 (= Q193), K194 (= K194)

O32148 (S)-ureidoglycine--glyoxylate transaminase; UGXT; (S)-ureidoglycine--glyoxylate aminotransferase; Purine catabolism protein PucG; EC 2.6.1.112 from Bacillus subtilis (strain 168) (see paper)
28% identity, 98% coverage: 7:384/384 of query aligns to 14:410/416 of O32148

query
sites
O32148

L

I

M

M

I

T

P

P

G

G

P

P

T

V

P

E

V

V

P

D

E

P

A

R

A

V

L

L

L

R

A

V

L

M

A

S

K

T

H

P

P

V

I

V

G

G

H
x
Q

R

F

T

D

S

P

E

A

F

F

S

T

N

G

I

I

L

M

A

N

E

E

V

T

T

M

E

E

N

M

L

L

K

R

W

E

L

L

H

F

Q

Q

T

T

Q

K

T

N

D

R

-

W

V

A

L

Y

T

P

L

I

N

D

V

G

S

T

G

S

T

R

G

A

A

G

V

I

E

E

A

A

G

V

I

L

I

A

N

S

F

V

L

I

S

E

P

P

G

E

D

D

R

D

I

V

L

L

V

I

G

P

S

I

N

Y

G

G

K

R

F

F

G

G

E

Y

R

L

W

L

V

T

E

E

V

I

G

A

Q

E

A

R

Y

Y

G

G

L

A

N

N

V

V

E

H

E

M

V

L

K

E

V

C

E

E

W

W

G

G

K

T

P

V

L

F

D

D

P

P

A

E

V

D

F

I

A

I

E

R

K

E

L

I

Q

K

A

-

D

-

T

-

Q

K

K

V

Q

K

I

P

K

K

A

I

V

V

I

A

I

M

T

V

H

H

S

G

E

E

T

T

S

S

T

T

G

G

V

R

L

I

N

H

D

P

L

L

E

K

S

A

I

I

N

G

R

E

H

A

V

C

K

R

A

T

H

E

G

-

E

D

A

A

L

L

I

F

I

I

V

V

D

D

A

A

V

V

T

A

S

T

L

I

G

G

A

G

F

C

N

E

L

V

P

K

V

V

D

D

A

E

W

W

G

K

L

I

D

D

I

A

V

A

A

I

S

G

G

G

S

T

Q

Q

K
|
K

G

C

Y

L

M

S

I

V

P

P

P

S

G

G

L

M

G

A

F

P

V

I

S

T

V

Y

S

N

P

E

K

R

A

V

W

A

E

D

A

V

Y

I

K

A

T

A

A

R

K

K

-

K

-

V

-

E

-

R

-

G

-

I

-

A

-

T

-

Q

-

A

-

D

-

R

-

A

-

L

-

S

-

G

-

N

-

R

-

P

L

I

P

T

K

S

Y

N

Y

Y

L

F

D

D

L

L

G

S

K

Q

Y

L

R

E

K

D

A

Y

-

W

T

S

A

E

K

R

N

R

T

L

T
x
N

P

H

F

H

T

T

P

E

P

A

V

T

N

T

L

M

I

L

V

Y

A

A

L

L

H

R

T

E

T

G

L

V

R

R

I

L

M

V

K

L

E

E

G

G

L

L

E

E

S

T

I

R

F

F

A

E

R

R

H

H

E

R

R

H

L

H

K

E

N

A

A

A

T

L

R

A

A

A

A

G

I

I

Q

K

G

A

L

M

N

G

L

L

P

R

L

L

F

F

A

G

A

D

D

D

S

S

S

C

A

K

S

M

P

P

A

V

I

V

T

T

A

C

V

V

A

E

-

I

P

P

Q

G

G

G

I

I

E

D

S

G

D

E

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S

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M

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K

A

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-

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-

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A

Q37 (≠ H30) mutation to H: 5-fold decrease in transamination activity.
K198 (= K194) modified: N6-(pyridoxal phosphate)lysine
N264 (≠ T239) mutation to S: 9-fold decrease in transamination activity.; mutation to Y: Loss of transamination activity.

1j04A Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro (see paper)
33% identity, 89% coverage: 6:348/384 of query aligns to 21:368/387 of 1j04A

query
sites
1j04A

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A

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A

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I

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D

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A

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A

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A

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-

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-

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Y

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-

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binding (aminooxy)acetic acid: P25 (= P10), R357 (= R337)

5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
33% identity, 89% coverage: 6:348/384 of query aligns to 19:366/385 of 5hhyA

query
sites
5hhyA

M

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binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S76 (≠ T64), G77 (= G65), H78 (vs. gap), W103 (≠ F89), S153 (≠ T142), D178 (= D168), V180 (= V170), Q203 (= Q193), K204 (= K194), Y255 (vs. gap), T258 (= T242)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
33% identity, 89% coverage: 6:348/384 of query aligns to 24:371/392 of P21549

query
sites
P21549

M

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x
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A

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x
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R36 (≠ A18) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
G41 (≠ L23) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; associated in cis with L-11 and M-340; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
G47 (= G29) to R: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
G82 (= G65) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
W108 (≠ F89) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
A112 (≠ W93) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
L150 (≠ A134) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
F152 (≠ I136) to I: in HP1; associated in cis with L-11 and M-340; results in protein destabilization; no loss of dimerization; decreased alanine--glyoxylate aminotransferase activity; loss of alanine--glyoxylate aminotransferase activity when associated with L-11 and M-340; mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908524
G156 (≠ S140) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
S158 (≠ T142) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
G161 (= G145) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
L166 (= L150) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
G170 (≠ N154) to R: in HP1; associated in cis with L-11 and M-340; decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; results in mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908529
C173 (≠ V157) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
D183 (= D168) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
S187 (= S172) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
I202 (= I187) to N: in HP1; uncertain significance; dbSNP:rs536352238
S205 (= S190) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
K209 (= K194) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
S218 (≠ G203) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
R233 (≠ A218) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
I244 (≠ L227) to T: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity in vitro; no loss of dimerization; partial mitochondrial mistargeting; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; dbSNP:rs121908525
C253 (≠ K236) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
I279 (≠ M258) to T: in dbSNP:rs140992177
A280 (≠ K259) to V: in dbSNP:rs73106685
V326 (vs. gap) to I: in dbSNP:rs115057148
I340 (≠ K317) to M: in allele minor; associated in cis with L-11; no effect on alanine--glyoxylate aminotransferase activity in vitro; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with L-11; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and I-152; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and R-170; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and T-244; results in mitochondrial mistargeting when associated with L-11 and R-170; dbSNP:rs4426527

Sites not aligning to the query:

9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
11 P → L: in allele minor; associated in cis with M-340; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with I-152 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with R-170 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with T-244 and M-340; causes mitochondrial mistargeting when associated with R-170 and M-340; dbSNP:rs34116584

6rv0A Human alanine:glyoxylate aminotransferase major allele (agt-ma); with pmp in the active site (see paper)
33% identity, 89% coverage: 6:348/384 of query aligns to 19:366/384 of 6rv0A

query
sites
6rv0A

M

L

L

L

M

L

I

G

P

P

G

G

P

P

T

S

P

N

V

L

P

P

E

P

A

R

A

I

L

M

L

A

A

A

L

G

A

G

K

L

H

Q

P

M

I

I

G

G

H

S

R

M

T

S

S

K

E

D

F

M

S

Y

N

Q

I

I

L

M

A

D

E

E

V

I

T

K

E

E

N

G

L

I

K

Q

W

Y

L

V

H

F

Q

Q

T

T

Q

R

T

-

D

N

V

P

L

L

T

T

L

L

N

V

V

I

S

S

G

G

T
x
S

G
|
G

-
x
H

-

C

A

A

V

L

E

E

A

A

G

A

I

L

I

V

N

N

F

V

L

L

S

E

P

P

G

G

D

D

R

S

I

F

L

L

V

V

G

G

S

A

N

N

G

G

K

I

F
x
W

G

G

E

Q

R

R

W

A

V

V

E

D

V

I

G

G

Q

E

A

R

Y

I

G

G

L

A

N

R

V

V

E

H

E

P

V

M

K

T

V

K

E

D

W

-

G

-

K

-

P

P

L

G

D

G

P

H

A

Y

V

T

F

L

A

Q

E

E

K

V

L

E

Q

E

A

G

D

L

T

A

Q

Q

K

H

Q

K

I

P

K

V

A

L

V

L

I

F

I

L

T

T

H

H

S

G

E

E

T

S

S

S

T

T

G

G

V

V

L

L

N

Q

D

P

L

L

E

D

S

G

I

F

N

G

R

E

H

L

V

C

K

H

A

R

H

Y

G

-

E

K

A

C

L

L

I

L

V

V

D
|
D

A

S

V
|
V

T
x
A

S

S

L

L

G

G

A

G

F

T

N

P

L

L

P

Y

V

M

D

D

A

R

W

Q

G

G

L

I

D

D

I

I

V

L

A

Y

S

S

G

G

S

S

Q

Q

K
|
K

G

A

Y

L

M

N

I

A

P

P

G

G

L

T

G

S

F

L

V

I

S

S

V

F

S

S

P

D

K

K

A

A

W

K

E

K

A

K

Y

M

K

Y

T

S

A

R

K

K

L

T

P

K

-

P

-

F

K

S

Y

F

Y

Y

L

L

D

D

L

I

G

K

K

W

Y

L

R

A

K

N

A

F

T

W

A

G

K

C

N

D

T

D

T

Q

P

P

-

R

-

M

-

Y

-

H

F

H

T

T

P

I

P

P

V

V

N

I

L

S

I

L

V

Y

A

S

L

L

H

R

T

E

T

S

L

L

R

A

I

L

M

I

K

A

E

E

E

Q

G

G

L

L

E

E

S

N

I

S

F

W

A

R

R

Q

H

H

E

R

R

E

L

A

K

A

N

A

A

Y

T

L

R

H

A

G

A

R

I

L

Q

Q

G

A

L

L

N

G

L

L

P

Q

L

L

F

F

A

V

A

K

D

D

S

P

S

A

A

L

S

R

-

L

P

P

A

T

I

V

T

T

A

T

V

V

A

A

-

V

P

P

Q

A

G

G

I

Y

E

D

S

W

D

R

K

D

I

I

R

V

S

S

L

Y

M

V

K

I

K

D

R

H

F

F

D

D

I

I

A

E

L

I

A

M

G

G

Q

L

D

G

H

P

L

S

S

T

N

G

K

K

I

V

F

L

R

R

I

I

G

G

H

L

L

L

G

G

F

C

V

N

S

A

D

T

R

R

D

E

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S76 (≠ T64), G77 (= G65), H78 (vs. gap), W103 (≠ F89), D178 (= D168), V180 (= V170), A181 (≠ T171), K204 (= K194)

1h0cA The crystal structure of human alanine:glyoxylate aminotransferase (see paper)
32% identity, 89% coverage: 6:348/384 of query aligns to 21:366/385 of 1h0cA

query
sites
1h0cA

M

L

L

L

M

L

I

G

P
|
P

G
|
G

P

P

T

S

P

N

V

L

P

P

E

P

A

R

A

I

L

M

L

A

A

A

L

G

A

G

K

L

H

Q

P

M

I

I

G

G

H

S

R

M

T

S

S

K

E

D

F

M

S

Y

N

Q

I

I

L

M

A

D

E

E

V

I

T

K

E

E

N

G

L

I

K

Q

W

Y

L

V

H

F

Q

Q

T

T

Q

R

T

-

D

N

V

P

L

L

T

T

L

L

N

V

V

I

S

S

G

G

T
x
S

G
|
G

-
x
H

-

C

A

A

V

L

E

E

A

A

G

A

I

L

I

V

N

N

F

V

L

L

S

E

P

P

G

G

D

D

R

S

I

F

L

L

V

V

G

G

S

A

N

N

G

G

K

I

F
x
W

G

G

E

Q

R

R

W

A

V

V

E

D

V

I

G

G

Q

E

A

R

Y

I

G

G

L

V

N

H

V

-

E

-

V

-

K

-

V

-

E

-

W

-

G

-

K

-

P

P

L

M

-

T

-

K

D

D

P

P

A

G

-

G

-

H

-

Y

V

T

F

L

A

Q

E

E

K

V

L

E

Q

E

A

G

D

L

T

A

Q

Q

K

H

Q

K

I

P

K

V

A

L

V

L

I

F

I

L

T

T

H

H

S

G

E

E

T
x
S

S

S

T

T

G

G

V

V

L

L

N

Q

D

P

L

L

E

D

S

G

I

F

N

G

R

E

H

L

V

C

K

H

A

R

H

Y

G

-

E

K

A

C

L

L

I

L

V

V

D
|
D

A

S

V
|
V

T

A

S

S

L

L

G

G

A

G

F

T

N

P

L

L

P

Y

V

M

D

D

A

R

W

Q

G

G

L

I

D

D

I

I

V

L

A

Y

S

S

G

G

S

S

Q

Q

K
|
K

G

A

Y

L

M

N

I

A

P

P

G

G

L

T

G

S

F

L

V

I

S

S

V

F

S

S

P

D

K

K

A

A

W

K

E

K

A

K

Y

M

K

Y

T

S

A

R

K

K

L

T

P

K

-

P

-

F

K

S

Y

F

Y

Y

L

L

D

D

L

I

G

K

K

W

Y

L

R

A

K

N

A

F

T

W

A

G

K

C

N

D

T

D

T

Q

P

P

-

R

-

M

-

Y

-

H

F

H

T

T

P

I

P

P

V

V

N

I

L

S

I

L

V

Y

A

S

L

L

H

R

T

E

T

S

L

L

R

A

I

L

M

I

K

A

E

E

E

Q

G

G

L

L

E

E

S

N

I

S

F

W

A

R

R

Q

H

H

E

R

R

E

L

A

K

A

N

A

A

Y

T

L

R

H

A

G

A

R

I

L

Q

Q

G

A

L

L

N

G

L

L

P

Q

L

L

F

F

A

V

A

K

D

D

S

P

S

A

A

L

S

R

-

L

P

P

A

T

I

V

T

T

A

T

V

V

A

A

-

V

P

P

Q

A

G

G

I

Y

E

D

S

W

D

R

K

D

I

I

R

V

S

S

L

Y

M

V

K

I

K

D

R

H

F

F

D

D

I

I

A

E

L

I

A

M

G

G

Q
x
L

D

G

H

P

L

S

S

T

N

G

K

K

I

V

F

L

R
|
R

I

I

G

G

H

L

L

L

G

G

F

C

V

N

S

A

D

T

R

R

D

E

binding (aminooxy)acetic acid: P25 (= P10), G26 (= G11), L346 (≠ Q328), R355 (= R337)
binding pyridoxal-5'-phosphate: S78 (≠ T64), G79 (= G65), H80 (vs. gap), W105 (≠ F89), S153 (≠ T142), D178 (= D168), V180 (= V170), K204 (= K194)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 385

3zrqA Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from sulfolobus solfataricus (see paper)
32% identity, 99% coverage: 3:381/384 of query aligns to 1:379/382 of 3zrqA

query
sites
3zrqA

D

D

K

K

L

L

M

L

L

L

M

H

I

V

P

-

G

G

P

P

T

T

P

T

V

I

P

K

E

E

A

D

A

V

L

L

L

V

A

A

L

G

A

L

K

E

H

N

P

N

I

V

G

G

H

F

R

T

T

S

S

K

E

E

F

F

S

V

N

E

I

A

L

L

A

A

E

Y

V

S

T

L

E

K

N

G

L

L

K

R

W

Y

L

V

H

M

Q

G

T

A

Q

S

T

K

D

N

V

Y

L

Q

T

P

L

L

N

I

V

I

-

P

-

G

S

G

G
|
G

T
|
T

G

S

A

A

V

M

E

E

A

S

G

-

I

V

I

T

N

S

F

L

L

L

S

K

P

P

G

N

D

D

R

K

I

I

L

L

V

V

G

V

S

S

N

N

G

G

K

V

F
|
F

G

G

E

D

R

R

W

W

V

E

E

Q

V

I

G

F

Q

K

A

R

Y

Y

G

P

L

V

N

N

V

V

E

K

E

V

V

L

K

R

V

P

E

S

W

P

G

G

K

D

P

Y

L

V

D

K

P

P

A

G

V

E

F

V

A

E

E

E

K

E

L

V

Q

R

A

-

D

-

T

-

Q

K

K

S

Q

E

I

Y

K

K

A

L

V

V

I

A

I

L

T

T

H

H

S

V

E

E

T
|
T

S

S

T

T

G

G

V

V

L

R

N

E

D

P

L

V

E

K

S

D

I

V

N

I

R

N

H

K

V

I

K

R

A

K

H

Y

G

V

E

E

A

-

L

L

I

I

I

V

V

V

D
|
D

A

G

V
|
V

T

S

S

S

L

V

G

G

A

A

F

E

N

E

L

V

P

K

V

A

D

E

A

E

W

W

G

N

L

V

D

D

I

V

V

Y

A

L

S

T

G

A

S

S

Q

Q

K
|
K

G

A

Y

L

M

G

I

S

P

A

G

G

L

L

G

G

F

L

V

L

S

L

V

L

S

S

P

P

K

K

A

A

W

L

E

S

A

I

Y

L

K

D

T

S

A

Q

K

N

-

S

L

I

P

A

K

G

Y

Y

L

L

D

D

L

L

G

R

K

N

Y

W

-

L

-

P

-

V

-

M

R

R

K

G

A

A

T

E

A

E

K

G

N

K

T

A

P
x
Y

F

F

-

A

T
|
T

P

P

V

V

N

H

L

V

I

I

V

L

A

Q

L

L

H

A

T

E

T

A

L

F

R

R

I

L

M

I

K

E

E

K

E

E

G

G

L

I

E

E

S

N

I

R

F

I

A

K

R

R

H

H

E

T

R

M

L

V

K

A

N

S

A

A

T

I

R

R

A

A

A

G

I

L

Q

E

G

A

L

L

N

G

L

L

P

E

L

I

F

V

A

A

A

R

D

R

-

P

S

E

S

S

A

Y

S

S

P

N

A

T

I

V

T

T

A

G

V

V

A

I

P

L

Q

K

G

V

I

A

E

D

S

P

D

Q

K

K

I

V

R

L

S

A

L

G

M

T

K

V

K

N

R

E

F

-

D

G

I

V

A

E

L

F

A

A

G

P

G

G

Q

V

D

-

H

H

L

P

S

A

N

F

K

K

I

Y

F

F

R

R

I

I

G

G

H

H

L

M

G

G

F

W

V

V

S

T

D

P

R

N

D

D

I

A

L

I

S

I

C

A

I

I

A

S

S

V

L

I

E

E

V

R

T

T

L

L

T

R

E

K

L

L

G

G

Y

-

E

E

D

P

F

I

T

R

P

F

G

G

S

E

G

G

I

V

A

K

A

A

A

V

V

E

K

E

V

V

F

L

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G62 (= G63), T63 (= T64), F87 (= F89), T137 (= T142), D162 (= D168), V164 (= V170), K188 (= K194), Y239 (≠ P240), T242 (= T242)

2huuA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with alanine (see paper)
31% identity, 88% coverage: 6:342/384 of query aligns to 21:361/385 of 2huuA

query
sites
2huuA

M

L

L

L

M

M

I

G

P
|
P

G

G

P

P

T

S

P

N

V

A

P

P

E

Q

A

R

A

V

L

L

L

D

A

A

L

M

A

S

K

R

H

P

P

I

I

L

G

G

H
|
H

R

L

T

H

S

P

E

E

F

T

S

L

N

K

I

I

L

M

A

D

E

D

V

I

T

K

E

E

N

G

L

V

K

R

W

Y

L

L

H

F

Q

Q

T

T

Q

N

T

N

-

I

D

A

V

T

L

F

T

C

L

L

N

S

V

A

S

S

G

G

T

H

G

G

A

G

V

M

E

E

A

A

G

T

I

L

I

C

N

N

F

L

L

L

S

E

P

D

G

G

D

D

R

V

I

I

L

L

V

I

G

G

S

H

N

T

G

G

K

H

F

W

G

G

E

D

R

R

W

S

V

A

E

D

V

M

G

A

Q

T

A

R

Y

Y

G

G

L

A

N

D

V

V

E

R

E

V

V

V

K

K

V

S

E

K

W

V

G

G

K

Q

P

S

L

L

D

S

P

-

A

-

V

-

F

L

A

D

E

E

K

I

L

R

Q

D

A

A

D

L

T

L

Q

I

K

H

Q

K

I

P

K

S

A

V

V

L

I

F

I

L

T

T

H

Q

S

G

E

D

T
x
S

S

S

T

T

G

G

V

V

L

L

N

Q

D

G

L

L

E

E

S

G

I

V

N

G

R

A

H

L

V

C

K

H

A

Q

H

H

G

-

E

N

A

C

L

L

I

L

I

I

V

V

D

D

A

T

V

V

T

A

S

S

L

L

G

G

A

G

F

A

N

P

L

M

P

F

V

M

D

D

A

R

W

W

G

E

L

I

D

D

I

A

V

M

A

Y

S

T

G

G

S

S

Q

Q

K
|
K

G

V

Y

L

M

G

I

A

P

P

G

G

L

I

G

T

F

P

V

V

S

S

V

F

S

S

P

H

K

R

A

A

W

V

E

E

A

R

Y

Y

K

K

-

R

T

N

A

T

K

K

L

V

P

K

K

V

Y

Y

L

W

D

D

L

M

-

S

-

L

-

V

G

G

K

D

Y

Y

R

W

K

G

A

C

T

F

A

G

K

R

-

P

N

R

T

I

T
x
Y

P

H

F

H

T

T

P

I

P

S

V

S

N

T

L

L

I

L

V

Y

A

G

L

L

H

R

T

E

T

A

L

I

R

A

I

M

M

A

K

C

E

E

G

G

L

L

E

P

S

A

I

L

F

I

A

A

R

R

H

H

E

E

R

D

L

C

K

A

N

K

A

R

T

L

R

Y

A

R

A

G

I

L

Q

Q

G

D

L

A

N

G

L

F

P

E

L

L

F

Y

A

A

A

D

D

P

S

K

S

D

A

R

S

L

P

S

A

T

I

V

T

T

A

T

V

I

-

K

A

V

P

P

Q

Q

G

G

I

V

E

D

S

W

D

L

K

K

I

A

R

A

S

Q

L

Y

M

A

K

M

K

K

R

T

F

Y

D

L

I

V

A

E

L

I

A

S

G

G

Q
x
L

D

G

H

P

L

T

S

A

N

G

K

Q

I

V

F

F

R
|
R

I

I

G

G

H

L

L

M

G

G

binding 1-butanol: H45 (= H30), Y257 (≠ T239)
binding alanine: P25 (= P10), S155 (≠ T142), K206 (= K194), L347 (≠ Q328), R356 (= R337)

2huiA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with glyoxylic acid (see paper)
31% identity, 88% coverage: 6:342/384 of query aligns to 21:361/385 of 2huiA

query
sites
2huiA

M

L

L

L

M

M

I

G

P
|
P

G

G

P

P

T

S

P

N

V

A

P

P

E

Q

A

R

A

V

L

L

L

D

A

A

L

M

A

S

K

R

H

P

P

I

I

L

G

G

H

H

R

L

T

H

S

P

E

E

F

T

S

L

N

K

I

I

L

M

A

D

E

D

V

I

T

K

E

E

N

G

L

V

K

R

W

Y

L

L

H

F

Q

Q

T

T

Q

N

T

N

-

I

D

A

V

T

L

F

T

C

L

L

N

S

V

A

S

S

G

G

T

H

G

G

A

G

V

M

E

E

A

A

G

T

I

L

I

C

N

N

F

L

L

L

S

E

P

D

G

G

D

D

R

V

I

I

L

L

V

I

G

G

S

H

N

T

G

G

K

H

F

W

G

G

E

D

R

R

W

S

V

A

E

D

V

M

G

A

Q

T

A

R

Y

Y

G

G

L

A

N

D

V

V

E

R

E

V

V

V

K

K

V

S

E

K

W

V

G

G

K

Q

P

S

L

L

D

S

P

-

A

-

V

-

F

L

A

D

E

E

K

I

L

R

Q

D

A

A

D

L

T

L

Q

I

K

H

Q

K

I

P

K

S

A

V

V

L

I

F

I

L

T

T

H

Q

S

G

E

D

T
x
S

S

S

T

T

G

G

V

V

L

L

N

Q

D

G

L

L

E

E

S

G

I

V

N

G

R

A

H

L

V

C

K

H

A

Q

H

H

G

-

E

N

A

C

L

L

I

L

I

I

V

V

D

D

A

T

V

V

T

A

S

S

L

L

G

G

A

G

F

A

N

P

L

M

P

F

V

M

D

D

A

R

W

W

G

E

L

I

D

D

I

A

V

M

A

Y

S

T

G

G

S

S

Q

Q

K
|
K

G

V

Y

L

M

G

I

A

P

P

G

G

L

I

G

T

F

P

V

V

S

S

V

F

S

S

P

H

K

R

A

A

W

V

E

E

A

R

Y

Y

K

K

-

R

T

N

A

T

K

K

L

V

P

K

K

V

Y

Y

L

W

D

D

L

M

-

S

-

L

-

V

G

G

K

D

Y

Y

R

W

K

G

A

C

T

F

A

G

K

R

-

P

N

R

T

I

T

Y

P

H

F

H

T

T

P

I

P

S

V

S

N

T

L

L

I

L

V

Y

A

G

L

L

H

R

T

E

T

A

L

I

R

A

I

M

M

A

K

C

E

E

G

G

L

L

E

P

S

A

I

L

F

I

A

A

R

R

H

H

E

E

R

D

L

C

K

A

N

K

A

R

T

L

R

Y

A

R

A

G

I

L

Q

Q

G

D

L

A

N

G

L

F

P

E

L

L

F

Y

A

A

A

D

D

P

S

K

S

D

A

R

S

L

P

S

A

T

I

V

T

T

A

T

V

I

-

K

A

V

P

P

Q

Q

G

G

I

V

E

D

S

W

D

L

K

K

I

A

R

A

S

Q

L

Y

M

A

K

M

K

K

R

T

F

Y

D

L

I

V

A

E

L

I

A

S

G

G

Q

L

D

G

H

P

L

T

S

A

N

G

K

Q

I

V

F

F

R
|
R

I

I

G

G

H

L

L

M

G

G

binding glyoxylic acid: P25 (= P10), S155 (≠ T142), K206 (= K194), R356 (= R337)

2hufA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase (see paper)
31% identity, 88% coverage: 6:342/384 of query aligns to 21:361/385 of 2hufA

query
sites
2hufA

M

L

L

L

M

M

I

G

P

P

G

G

P

P

T

S

P

N

V

A

P

P

E

Q

A

R

A

V

L

L

L

D

A

A

L

M

A

S

K

R

H

P

P

I

I

L

G

G

H
|
H

R

L

T

H

S

P

E

E

F

T

S

L

N

K

I

I

L

M

A

D

E

D

V

I

T

K

E

E

N

G

L

V

K

R

W

Y

L

L

H

F

Q

Q

T

T

Q

N

T

N

-

I

D

A

V

T

L

F

T

C

L

L

N

S

V

A

S

S

G

G

T

H

G

G

A

G

V

M

E

E

A

A

G

T

I

L

I

C

N

N

F

L

L

L

S

E

P

D

G

G

D

D

R

V

I

I

L

L

V

I

G

G

S

H

N

T

G

G

K

H

F

W

G

G

E

D

R

R

W

S

V

A

E

D

V

M

G

A

Q

T

A

R

Y

Y

G

G

L

A

N

D

V

V

E

R

E

V

V

V

K

K

V

S

E

K

W

V

G

G

K

Q

P

S

L

L

D

S

P

-

A

-

V

-

F

L

A

D

E

E

K

I

L

R

Q

D

A

A

D

L

T

L

Q

I

K

H

Q

K

I

P

K

S

A

V

V

L

I

F

I

L

T

T

H

Q

S

G

E

D

T
x
S

S

S

T

T

G

G

V

V

L

L

N

Q

D

G

L

L

E

E

S

G

I

V

N

G

R

A

H

L

V

C

K

H

A

Q

H

H

G

-

E

N

A

C

L

L

I

L

I

I

V

V

D

D

A

T

V

V

T

A

S

S

L

L

G

G

A

G

F

A

N

P

L

M

P

F

V

M

D

D

A

R

W

W

G

E

L

I

D

D

I

A

V

M

A

Y

S

T

G

G

S

S

Q

Q

K

K

G

V

Y

L

M

G

I

A

P

P

G

G

L

I

G

T

F

P

V

V

S

S

V

F

S

S

P

H

K

R

A

A

W

V

E

E

A

R

Y

Y

K

K

-

R

T

N

A

T

K

K

L

V

P

K

K

V

Y

Y

L

W

D

D

L

M

-

S

-

L

-

V

G

G

K

D

Y

Y

R

W

K

G

A

C

T

F

A

G

K

R

-

P

N

R

T

I

T
x
Y

P

H

F

H

T

T

P

I

P

S

V

S

N

T

L

L

I

L

V

Y

A

G

L

L

H

R

T

E

T

A

L

I

R

A

I

M

M

A

K

C

E

E

G

G

L

L

E

P

S

A

I

L

F

I

A

A

R

R

H

H

E

E

R

D

L

C

K

A

N

K

A

R

T

L

R

Y

A

R

A

G

I

L

Q

Q

G

D

L

A

N

G

L

F

P

E

L

L

F

Y

A

A

A

D

D

P

S

K

S

D

A

R

S

L

P

S

A

T

I

V

T

T

A

T

V

I

-

K

A

V

P

P

Q

Q

G

G

I

V

E

D

S

W

D

L

K

K

I

A

R

A

S

Q

L

Y

M

A

K

M

K

K

R

T

F

Y

D

L

I

V

A

E

L

I

A

S

G

G

Q

L

D

G

H

P

L

T

S

A

N

G

K

Q

I

V

F

F

R
|
R

I

I

G

G

H

L

L

M

G

G

binding 1-butanol: H45 (= H30), S155 (≠ T142), Y257 (≠ T239), R356 (= R337)

Q3LSM4 Alanine--glyoxylate aminotransferase; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (see paper)
31% identity, 88% coverage: 6:342/384 of query aligns to 21:361/393 of Q3LSM4

query
sites
Q3LSM4

M

L

L

L

M

M

I

G

P

P

G

G

P

P

T

S

P

N

V

A

P

P

E

Q

A

R

A

V

L

L

L

D

A

A

L

M

A

S

K

R

H

P

P

I

I

L

G

G

H

H

R

L

T

H

S

P

E

E

F

T

S

L

N

K

I

I

L

M

A

D

E

D

V

I

T

K

E

E

N

G

L

V

K

R

W

Y

L

L

H

F

Q

Q

T

T

Q

N

T

N

-

I

D

A

V

T

L

F

T

C

L

L

N

S

V

A

S
|
S

G
|
G

T
x
H

G

G

A

G

V

M

E

E

A

A

G

T

I

L

I

C

N

N

F

L

L

L

S

E

P

D

G

G

D

D

R

V

I

I

L

L

V

I

G

G

S

H

N

T

G

G

K

H

F

W

G

G

E

D

R

R

W

S

V

A

E

D

V

M

G

A

Q

T

A

R

Y

Y

G

G

L

A

N

D

V

V

E

R

E

V

V

V

K

K

V

S

E

K

W

V

G

G

K

Q

P

S

L

L

D

S

P

-

A

-

V

-

F

L

A

D

E

E

K

I

L

R

Q

D

A

A

D

L

T

L

Q

I

K

H

Q

K

I

P

K

S

A

V

V

L

I

F

I

L

T

T

H

Q

S

G

E

D

T
x
S

S

S

T

T

G

G

V

V

L

L

N

Q

D

G

L

L

E

E

S

G

I

V

N

G

R

A

H

L

V

C

K

H

A

Q

H

H

G

-

E

N

A

C

L

L

I

L

I

I

V

V

D

D

A

T

V

V

T

A

S

S

L

L

G

G

A

G

F

A

N

P

L

M

P

F

V

M

D

D

A

R

W

W

G

E

L

I

D

D

I

A

V

M

A

Y

S

T

G

G

S

S

Q
|
Q

K
|
K

G

V

Y

L

M

G

I

A

P

P

G

G

L

I

G

T

F

P

V

V

S

S

V

F

S

S

P

H

K

R

A

A

W

V

E

E

A

R

Y

Y

K

K

-

R

T

N

A

T

K

K

L

V

P

K

K

V

Y

Y

L

W

D

D

L

M

-

S

-

L

-

V

G

G

K

D

Y

Y

R

W

K

G

A

C

T

F

A

G

K

R

-

P

N

R

T

I

T
x
Y

P

H

F

H

T
|
T

P

I

P

S

V

S

N

T

L

L

I

L

V

Y

A

G

L

L

H

R

T

E

T

A

L

I

R

A

I

M

M

A

K

C

E

E

G

G

L

L

E

P

S

A

I

L

F

I

A

A

R

R

H

H

E

E

R

D

L

C

K

A

N

K

A

R

T

L

R

Y

A

R

A

G

I

L

Q

Q

G

D

L

A

N

G

L

F

P

E

L

L

F

Y

A

A

A

D

D

P

S

K

S

D

A

R

S

L

P

S

A

T

I

V

T

T

A

T

V

I

-

K

A

V

P

P

Q

Q

G

G

I

V

E

D

S

W

D

L

K

K

I

A

R

A

S

Q

L

Y

M

A

K

M

K

K

R

T

F

Y

D

L

I

V

A

E

L

I

A

S

G

G

Q

L

D

G

H

P

L

T

S

A

N

G

K

Q

I

V

F

F

R
|
R

I

I

G

G

H

L

L

M

G

G

SGH 78:80 (≠ SGT 62:64) binding in other chain
S155 (≠ T142) binding glyoxylate; binding L-alanine
Q205 (= Q193) binding in other chain
K206 (= K194) modified: N6-(pyridoxal phosphate)lysine
Y257 (≠ T239) binding pyridoxal 5'-phosphate
T260 (= T242) binding pyridoxal 5'-phosphate
R356 (= R337) binding glyoxylate

3zrrA Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from sulfolobus solfataricus (see paper)
32% identity, 98% coverage: 7:381/384 of query aligns to 1:376/379 of 3zrrA

query
sites
3zrrA

L

L

M

L

I

H

P
x
V

G

G

P

P

T

T

P

T

V

I

P

K

E

E

A

D

A

V

L

L

L

V

A

A

L

G

A

L

K

E

H

N

P

N

I

V

G

G

H
x
F

R

T

T

S

S

K

E

E

F

F

S

V

N

E

I

A

L

L

A

A

E

Y

V

S

T

L

E

K

N

G

L

L

K

R

W

Y

L

V

H

M

Q

G

T

A

Q

S

T

K

D

N

V

Y

L

Q

T

P

L

L

N

I

V

I

-

P

-

G

S
x
G

G
|
G

T
|
T

G

S

A

A

V

M

E

E

A

S

G

-

I

V

I

T

N

S

F

L

L

L

S

K

P

P

G

N

D

D

R

K

I

I

L

L

V

V

G

V

S

S

N

N

G

G

K

V

F
|
F

G

G

E

D

R

R

W

W

V

E

E

Q

V

I

G

F

Q

K

A

R

Y

Y

G

P

L

V

N

N

V

V

E

K

E

V

V

L

K

R

V

P

E

S

W

P

G

G

K

D

P

Y

L

V

D

K

P

P

A

G

V

E

F

V

A

E

E

E

K

E

L

V

Q

R

A

-

D

-

T

-

Q

K

K

S

Q

E

I

Y

K

K

A

L

V

V

I

A

I

L

T

T

H

H

S

V

E

E

T
|
T

S

S

T

T

G

G

V

V

L

R

N

E

D

P

L

V

E

K

S

D

I

V

N

I

R

N

H

K

V

I

K

R

A

K

H

Y

G

V

E

E

A

-

L

L

I

I

I

V

V

V

D
|
D

A

G

V
|
V

T

S

S

S

L

V

G

G

A

A

F

E

N

E

L

V

P

K

V

A

D

E

A

E

W

W

G

N

L

V

D

D

I

V

V

Y

A

L

S

T

G

A

S

S

Q

Q

K

K

G

A

Y

L

M

G

I

S

P

A

G

G

L

L

G

G

F

L

V

L

S

L

V

L

S

S

P

P

K

K

A

A

W

L

E

S

A

I

Y

L

K

D

T

S

A

Q

K

N

-

S

L

I

P

A

K

G

Y

Y

L

L

D

D

L

L

G

R

K

N

Y

W

-

L

-

P

-

V

-

M

R

R

K

G

A

A

T

E

A

E

K

G

N

K

T

A

P
x
Y

F

F

-

A

T
|
T

P

P

V

V

N

H

L

V

I

I

V

L

A

Q

L

L

H

A

T

E

T

A

L

F

R

R

I

L

M

I

K

E

E

K

E

E

G

G

L

I

E

E

S

N

I

R

F

I

A

K

R

R

H

H

E

T

R

M

L

V

K

A

N

S

A

A

T

I

R

R

A

A

A

G

I

L

Q

E

G

A

L

L

N

G

L

L

P

E

L

I

F

V

A

A

A

R

D

R

-

P

S

E

S

S

A

Y

S

S

P

N

A

T

I

V

T

T

A

G

V

V

A

I

P

L

Q

K

G

V

I

A

E

D

S

P

D

Q

K

K

I

V

R

L

S

A

L

G

M

T

K

V

K

N

R

E

F

-

D

G

I

V

A

E

L

F

A

A

G

P

G

G

Q

V

D

-

H

H

L

P

S

A

N

F

K

K

I

Y

F

F

R
|
R

I

I

G

G

H

H

L

M

G

G

F

W

V

V

S

T

D

P

R

N

D

D

I

A

L

I

S

I

C

A

I

I

A

S

S

V

L

I

E

E

V

R

T

T

L

L

T

R

E

K

L

L

G

G

Y

-

E

E

D

P

F

I

T

R

P

F

G

G

S

E

G

G

I

V

A

K

A

A

A

V

V

E

K

E

V

V

F

L

binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: V4 (≠ P10), F24 (≠ H30), G58 (≠ S62), G59 (= G63), T60 (= T64), F84 (= F89), T134 (= T142), D159 (= D168), V161 (= V170), Y236 (≠ P240), T239 (= T242), R333 (= R337)

3zrpA Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from sulfolobus solfataricus (see paper)
32% identity, 97% coverage: 11:381/384 of query aligns to 4:375/377 of 3zrpA

query
sites
3zrpA

G

G

P

P

T

T

P

T

V

I

P

K

E

E

A

D

A

V

L

L

L

V

A

A

L

G

A

L

K

E

H

N

P

N

I

V

G

G

H

F

R

T

T

S

S

K

E

E

F

F

S

V

N

E

I

A

L

L

A

A

E

Y

V

S

T

L

E

K

N

G

L

L

K

R

W

Y

L

V

H

M

Q

G

T

A

Q

S

T

K

D

N

V

Y

L

Q

T

P

L

L

N

I

V

I

-

P

-

G

S
x
G

G
|
G

T
|
T

G

S

A

A

V

M

E

E

A

S

G

-

I

V

I

T

N

S

F

L

L

L

S

K

P

P

G

N

D

D

R

K

I

I

L

L

V

V

G

V

S

S

N

N

G

G

K

V

F
|
F

G

G

E

D

R

R

W

W

V

E

E

Q

V

I

G

F

Q

K

A

R

Y

Y

G

P

L

V

N

N

V

V

E

K

E

V

V

L

K

R

V

P

E

S

W

P

G

G

K

D

P

Y

L

V

D

K

P

P

A

G

V

E

F

V

A

E

E

E

K

E

L

V

Q

R

A

-

D

-

T

-

Q

K

K

S

Q

E

I

Y

K

K

A

L

V

V

I

A

I

L

T

T

H

H

S

V

E

E

T
|
T

S

S

T

T

G

G

V

V

L

R

N

E

D

P

L

V

E

K

S

D

I

V

N

I

R

N

H

K

V

I

K

R

A

K

H

Y

G

V

E

E

A

-

L

L

I

I

I

V

V

V

D
|
D

A

G

V
|
V

T

S

S

S

L

V

G

G

A

A

F

E

N

E

L

V

P

K

V

A

D

E

A

E

W

W

G

N

L

V

D

D

I

V

V

Y

A

L

S

T

G

A

S

S

Q

Q

K
|
K

G

A

Y

L

M

G

I

S

P

A

G

G

L

L

G

G

F

L

V

L

S

L

V

L

S

S

P

P

K

K

A

A

W

L

E

S

A

I

Y

L

K

D

T

S

A

Q

K

N

-

S

L

I

P

A

K

G

Y

Y

L

L

D

D

L

L

G

R

K

N

Y

W

-

L

-

P

-

V

-

M

R

R

K

G

A

A

T

E

A

E

K

G

N

K

T

A

P
x
Y

F

F

-

A

T
|
T

P

P

V

V

N

H

L

V

I

I

V

L

A

Q

L

L

H

A

T

E

T

A

L

F

R

R

I

L

M

I

K

E

E

K

E

E

G

G

L

I

E

E

S

N

I

R

F

I

A

K

R

R

H

H

E

T

R

M

L

V

K

A

N

S

A

A

T

I

R

R

A

A

A

G

I

L

Q

E

G

A

L

L

N

G

L

L

P

E

L

I

F

V

A

A

A

R

D

R

-

P

S

E

S

S

A

Y

S

S

P

N

A

T

I

V

T

T

A

G

V

V

A

I

P

L

Q

K

G

V

I

A

E

D

S

P

D

Q

K

K

I

V

R

L

S

A

L

G

M

T

K

V

K

N

R

E

F

-

D

G

I

V

A

E

L

F

A

A

G

P

G

G

Q

V

D

-

H

H

L

P

S

A

N

F

K

K

I

Y

F

F

R

R

I

I

G

G

H

H

L

M

G

G

F

W

V

V

S

T

D

P

R

N

D

D

I

A

L

I

S

I

C

A

I

I

A

S

S

V

L

I

E

E

V

R

T

T

L

L

T

R

E

K

L

L

G

G

Y

-

E

E

D

P

F

I

T

R

P

F

G

G

S

E

G

G

I

V

A

K

A

A

A

V

V

E

K

E

V

V

F

L

binding pyridoxal-5'-phosphate: G57 (≠ S62), G58 (= G63), T59 (= T64), F83 (= F89), T133 (= T142), D158 (= D168), V160 (= V170), K184 (= K194), Y235 (≠ P240), T238 (= T242)

Query Sequence

>WP_012410502.1 NCBI__GCF_000020025.1:WP_012410502.1
MNDKLMLMIPGPTPVPEAALLALAKHPIGHRTSEFSNILAEVTENLKWLHQTQTDVLTLN
VSGTGAVEAGIINFLSPGDRILVGSNGKFGERWVEVGQAYGLNVEEVKVEWGKPLDPAVF
AEKLQADTQKQIKAVIITHSETSTGVLNDLESINRHVKAHGEALIIVDAVTSLGAFNLPV
DAWGLDIVASGSQKGYMIPPGLGFVSVSPKAWEAYKTAKLPKYYLDLGKYRKATAKNTTP
FTPPVNLIVALHTTLRIMKEEGLESIFARHERLKNATRAAIQGLNLPLFAADSSASPAIT
AVAPQGIESDKIRSLMKKRFDIALAGGQDHLSNKIFRIGHLGFVSDRDILSCIASLEVTL
TELGYEDFTPGSGIAAAVKVFSQS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory