SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012410949.1 NCBI__GCF_000020025.1:WP_012410949.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4rjkG Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
36% identity, 99% coverage: 4:543/547 of query aligns to 4:551/553 of 4rjkG

query
sites
4rjkG

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binding magnesium ion: D437 (= D432), D464 (= D459), T466 (≠ G461)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: E47 (= E47), Q110 (= Q110)
binding thiamine diphosphate: I384 (≠ V379), G385 (= G380), S386 (≠ A381), H387 (= H382), Q410 (≠ A405), L412 (≠ M407), G436 (= G431), D437 (= D432), G438 (= G433), G439 (= G434), T466 (≠ G461), Y467 (= Y462), D468 (≠ G463), M469 (≠ L464), V470 (≠ I465), Y533 (= Y530)

4rjiC Acetolactate synthase from bacillus subtilis bound to thdp - crystal form i (see paper)
36% identity, 99% coverage: 4:543/547 of query aligns to 5:552/555 of 4rjiC

query
sites
4rjiC

A

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binding magnesium ion: D438 (= D432), D465 (= D459), T467 (≠ G461)
binding thiamine diphosphate: P24 (= P23), E48 (= E47), P74 (= P73), S387 (≠ A381), H388 (= H382), Q411 (≠ A405), G437 (= G431), D438 (= D432), G439 (= G433), G440 (= G434), T467 (≠ G461), Y468 (= Y462), D469 (≠ G463), M470 (≠ L464), V471 (≠ I465), Y534 (= Y530)

4rjkF Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
36% identity, 99% coverage: 4:543/547 of query aligns to 4:551/552 of 4rjkF

query
sites
4rjkF

A

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E

E

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A

A

M

L

F

F

A

Q

P

P

V

I

T

T

K

K

W

Y

N

S

K

V

Q

E

I

V

V

Q

R

D

P

V

S

K

I

N

T

I

P

P

E

E

V

A

V

V

R

T

K

N

A

A

F

F

K

R

R

I

S

A

Q

S

S

A

E

G

K

Q

P

A

G

G

A

A

V

A

H

F

I

V

D

S

L

F

P

P

E

Q

N

D

I

V

A

V

A

N

M

E

P

V

V

T

E

N

G

T

K

K

P

N

L

V

R

R

K

A

D

V

N

A

S

A

E

P

K

K

T

L

Y

G

A

P

S

A

F

A

A

D

S

D

I

A

R

I

A

S

A

A

A

I

A

A

I

K

C

I

Q

Q

A

T

V

A

N

K

-

L

P

P

L

V

I

V

L

L

V

V

G

G

N

M

G

K

A

G

I

G

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R

A

P

Q

E

A

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I

D

K

A

A

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A

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K

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N

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I

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P

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F

A

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E

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Q

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A

K

A

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A

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Y

W

F

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-

R

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I

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F

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D

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F

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I

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G

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F

L

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E

N

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D

D

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A

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Y

D

D

L

P

I

I

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E

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Y

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D

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P

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K

K

F

W

W

N

N

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I

N

N

G

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E

D

I

R

P

T

I

I

V

I

H

H

I

L

G

D

V

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I

P

I

A

A

E

D

I

I

D

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H

S

A

Y

Y

I

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N

D

A

L

E

E

V

L

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I

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G

D

D

I

I

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D

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T

L

I

Y

N

E

H

I

I

-

E

-

H

-

D

-

A

-

V

-

K

L

V

K

E

L

F

A

A

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E

R

R

Q

E

G

Q

K

K

P

-

D

-

P

-

F

-

A

-

I

-

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-

L

-

R

-

S

-

D

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I

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R

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A

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D

D

Y

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Y

Y

A

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-

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P

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D

-

W

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-

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-

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P

P

Q

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E

L

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I

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Y

K

D

E

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L

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N

V

A

M

V

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D

P

D

D

H

D

V

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I

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C

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G

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x
S

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H

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A

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W

M

M

A

S

R

R

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Y

F

H

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C

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H

Y

S

E

P

P

N

L

T

T

C

L

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M

I

I

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S

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N

G

G

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M

A
x
Q

A

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x
L

G

G

I

V

A

A

I

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P

G

W

A

A

L

I

A

G

A

A

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L

L

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V

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K

P

P

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G

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E

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K

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V

A

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V

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T

S

G
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G

D
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D

G
|
G

F

F

M

L

M

F

N

S

S

A

Q

M

E

E

L

L

E

E

T

T

A

A

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R

V

L

G

K

T

A

P

P

F

I

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V

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H

I

I

I

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W

N

N

D
|
D

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S

G
x
T

Y
|
Y

G
x
D

L
x
M

I
x
V

E

A

W

F

K

Q

Q

Q

E

L

N

K

H

K

F

Y

G

N

K

R

G

-

N

-

S

T

S

S

F

A

V

V

H

D

F

F

S

G

N

N

P

I

D

D

F

I

V

V

K

K

Y

Y

A

A

E

E

S

S

M

F

G

G

L

A

K

T

G

G

Y

L

R

R

V

V

E

E

S

S

A

P

L

D

D

Q

L

L

I

A

P

D

T

V

L

L

K

R

E

Q

A

G

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M

A

N

Q

A

D

E

V

G

P

P

A

V

V

I

I

I

D

D

C

V

P

P

V

V

D

D

Y
|
Y

R

S

E

D

N

N

-

I

-

N

-

L

-

A

-

S

H

D

R

K

F

L

S

P

Q

K

K

E

A

F

G

G

E

E

L

L

binding magnesium ion: D437 (= D432), D464 (= D459), T466 (≠ G461)
binding pyruvic acid: A25 (≠ E25), K26 (≠ E26)
binding thiamine diphosphate: P23 (= P23), E47 (= E47), P73 (= P73), G385 (= G380), S386 (≠ A381), H387 (= H382), Q410 (≠ A405), L412 (≠ M407), G436 (= G431), D437 (= D432), G438 (= G433), G439 (= G434), T466 (≠ G461), Y467 (= Y462), D468 (≠ G463), M469 (≠ L464), V470 (≠ I465), Y533 (= Y530)

5dx6B Acetolactate synthase from klebsiella pneumoniae soaked with beta- fluoropyruvate
38% identity, 97% coverage: 4:533/547 of query aligns to 20:544/557 of 5dx6B

query
sites
5dx6B

A

A

E

D

L

L

L

V

V

V

Q

S

C

Q

L

L

E

E

N

A

E

Q

G

G

V

V

Q

R

Y

Q

I

V

F

F

G

G

L
x
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

H

K

V

V

L

F

E

D

A

S

L

L

K

L

H

D

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I

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K

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F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

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R

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I

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T

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G

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K

A

A

G

G

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C

A

L

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S

V

T
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T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

M

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

L

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D

E

G

G

A

D

P

P

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V

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A

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A

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H

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|
Q

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x
S

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D

D

L

T

V

V

A

A

M

M

F

F

A
x
S

P

P

V

V

T
|
T

K

K

W

Y

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A

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I

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E

I

V

V

T

R

A

P

P

S

D

I

A

T

L

P

A

E

E

V

V

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K

N

A

A

F

F

K

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Q

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R

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P

G

G

A

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A

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L

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E
x
Q

N

D

I

V

A

V

A

D

M

G

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P

V

V

E

S

G

G

K

K

P

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K

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N

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A

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Q

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G

A

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D

A

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A

V

A

A

A

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A

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A

A

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N

N

P

P

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I

I

F

L

L

V

L

G

G

N

L

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M

A

A

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A

A

V

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A

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E

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T

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N

H

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I

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T

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Y

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A

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A

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x
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R

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L

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x
Q

F

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G

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F

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N

L

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A

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D

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C

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I

V

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x
E

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P

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A

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M

W

W

N

N

R

-

N

S

G

G

E

N

I

A

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I

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V

V

H

H

I

I

G

D

V

V

S

L

P

P

A

A

E

Y

I

E

D

E

S

R

S

N

Y

Y

I

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P

P

N

D

A

V

E

E

V

L

V

V

G

G

D

D

I

I

S

A

D

G

S

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N

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K

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L

A

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Q

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I

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A

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A

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E

I

I

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L

A

R

D

D

Y

R

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Q

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D

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M

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x
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x
F

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A
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A

R
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R

H

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Y

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R

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M

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I

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S

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N

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x
Q

A

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M
|
M

G

G

I

V

A

A

I

L

P

P

G

W

A

A

L

I

A

G

A

A

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W

L

L

V

V

H

N

P

P

K

E

R

R

K

K

V

V

A

S

V

V

T

S

G
|
G

D
|
D

G
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G

G

G

F

F

M

L

M

Q

N

S

S

S

Q

M

E

E

L

L

E

E

T

T

A

A

L

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R

V

L

G

K

T

A

P

N

F

V

V

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H

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L

I

I

F

W

N

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D
|
D

G

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G
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G

Y
|
Y

G
x
N

L
x
M

I
x
V

E

A

W

I

K
x
Q

Q

E

E

E

N

K

H

K

F

Y

G

Q

K

R

G

-

N

-

S

L

S

S

F

G

V

V

H

E

F

F

S

G

N

P

P

M

D

D

F

F

V

K

K

A

Y

Y

A

A

E

E

S

S

M

F

G

G

L

A

K

K

G

G

Y

F

R

A

V

V

E

E

S

S

A

A

L

E

D

A

L

L

I

E

P

P

T

T

L

L

K

R

E

A

A

A

L

M

A

D

Q

V

D

D

V

G

P

P

A

A

V

V

I

V

D

A

C

I

P

P

V

V

D

D

Y
|
Y

R

R

E

D

N

N

active site: I38 (≠ L22), G40 (= G24), A41 (≠ E25), K42 (≠ E26), I43 (≠ N27), E63 (= E47), T86 (= T70), H125 (= H109), Q126 (= Q110), S127 (≠ Y111), Q175 (≠ E159), L268 (= L250), E295 (≠ S277), M392 (≠ V379), Q418 (≠ A405), M420 (= M407), D445 (= D432), D472 (= D459), G474 (= G461), Y475 (= Y462), M477 (≠ L464), V478 (≠ I465), Q481 (≠ K468), Y541 (= Y530)
binding 3-fluoro-2-oxopropanoic acid: G264 (≠ W246), R265 (≠ S247), Q272 (≠ D254), A400 (= A387), R401 (= R388), Y404 (≠ H391)
binding magnesium ion: S135 (≠ A119), T138 (= T122), D445 (= D432), D472 (= D459), G474 (= G461)
binding thiamine diphosphate: G393 (= G380), S394 (≠ A381), F395 (≠ H382), Q418 (≠ A405), M420 (= M407), G444 (= G431), D445 (= D432), G446 (= G433), D472 (= D459), G474 (= G461), Y475 (= Y462), N476 (≠ G463), M477 (≠ L464), V478 (≠ I465), Y541 (= Y530)

1ozgA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate (see paper)
38% identity, 97% coverage: 4:533/547 of query aligns to 9:541/549 of 1ozgA

query
sites
1ozgA

A

A

E

D

L

L

L

V

V

V

Q

S

C

Q

L

L

E

E

N

A

E

Q

G

G

V

V

Q

R

Y

Q

I

V

F

F

G

G

L
x
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

H

K

V

V

L

F

E

D

A

S

L

L

K

L

H

D

S

S

I

I

K

R

F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

R

R

L

I

T

T

G

G

K

K

A

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

M

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

L

S

D

E

G

G

A

D

P

P

L

V

V

V

A

A

I

L

T

G

G

G

Q

A

V

V

G

K

T

R

D

A

R

D

M

K

H

A

I

K

E

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S

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H
|
H

Q
|
Q

Y
x
S

L

M

D

D

L

T

V

V

A

A

M

M

F

F

A

S

P

P

V

V

T

T

K

K

W

Y

N

A

K

I

Q

E

I

V

V

T

R

A

P

P

S

D

I

A

T

L

P

A

E

E

V

V

R

S

K

N

A

A

F

F

K

R

R

A

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A

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E

S

Q

E

G

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R

P

P

G

G

A

S

V

A

H

F

I

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D

S

L

L

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E
x
Q

N

D

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A

V

A

D

M

G

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P

V

V

E

S

G

G

K

K

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K

A

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N

G

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G

A

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A

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P

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D

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D

A

Q

A

V

A

A

A

K

A

L

I

I

C

A

Q

Q

A

A

V

K

N

N

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P

L

I

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F

L

L

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L

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G

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L

G

M

A

A

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A

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A

A

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L

A

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E

L

T

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H

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A

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A

F

L

A

W
x
G

S
x
R

V

V

G

G

L
|
L

Q

F

Q

N

R

N

D
x
Q

F

A

I

G

T

D

C

R

G

L

F

L

D

Q

N

L

T

A

D

D

L

L

V

V

I

I

A

C

I

I

G

G

Y

Y

D

S

L

P

I

V

E

E

F

Y

S
x
E

P

P

K

A

K

M

W

W

N

N

R

-

N

S

G

G

E

N

I

A

P

T

I

L

V

V

H

H

I

I

G

D

V

V

S

L

P

P

A

A

E

Y

I

E

D

E

S

R

S

N

Y

Y

I

T

P

P

N

D

A

V

E

E

V

L

V

V

G

G

D

D

I

I

S

A

D

G

S

T

L

L

Y

N

E

K

I

L

L

A

K

Q

L

N

A

I

D

D

R

H

Q

R

G

L

K

V

P

L

D

S

P

P

F

Q

A

A

I

A

S

E

L

I

R

L

S

R

D

D

I
x
R

R

Q

A

H

D

Q

Y

R

E

E

-

L

-

D

-

R

Q

R

Y

G

A

A

H

Q

D

L

D

N

G

Q

F

F

P

A

I

L

K

H

P

P

Q

L

K

R

L

I

I

V

Y

R

D

A

L

M

R

Q

Q

D

V

I

M

V

G

N

P

S

D

D

D

V

I

T

V

L

I

T

S

V

D

D

V
x
M

G
|
G

A
x
S

H
x
F

K

H

M

I

W

W

M

I

A

A

R
|
R

H

Y

Y

L

H
x
Y

C

T

H

F

S

R

P

A

N

R

T

Q

C

V

I

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

A

T

M
|
M

G

G

I

V

A

A

I

L

P

P

G

W

A

A

L

I

A

G

A

A

K

W

L

L

V

V

H

N

P

P

K

E

R

R

K

K

V

V

A

S

V

V

T

S

G
|
G

D
|
D

G
|
G

G

G

F

F

M

L

M

Q

N

S

S

S

Q

M

E

E

L

L

E

E

T

T

A

A

L

V

R

R

V

L

G

K

T

A

P

N

F

V

V

L

T

H

I

L

I

I

F

W

N

V

D
|
D

G

N

G
|
G

Y
|
Y

G
x
N

L
x
M

I
x
V

E

A

W

I

K
x
Q

Q

E

E

E

N

K

H

K

F

Y

G

Q

K

R

G

-

N

-

S

L

S

S

F

G

V

V

H

E

F

F

S

G

N

P

P

M

D

D

F

F

V

K

K

A

Y

Y

A

A

E

E

S

S

M

F

G

G

L

A

K

K

G

G

Y

F

R

A

V

V

E

E

S

S

A

A

L

E

D

A

L

L

I

E

P

P

T

T

L

L

K

R

E

A

A

A

L

M

A

D

Q

V

D

D

V

G

P

P

A

A

V

V

I

V

D

A

C

I

P

P

V

V

D

D

Y
|
Y

R

R

E

D

N

N

active site: I27 (≠ L22), G29 (= G24), A30 (≠ E25), K31 (≠ E26), I32 (≠ N27), E52 (= E47), T75 (= T70), H114 (= H109), Q115 (= Q110), S116 (≠ Y111), Q164 (≠ E159), L257 (= L250), E284 (≠ S277), M389 (≠ V379), Q415 (≠ A405), M417 (= M407), D442 (= D432), D469 (= D459), G471 (= G461), Y472 (= Y462), M474 (≠ L464), V475 (≠ I465), Q478 (≠ K468), Y538 (= Y530)
binding 2-hydroxyethyl dihydrothiachrome diphosphate: M389 (≠ V379), G390 (= G380), S391 (≠ A381), F392 (≠ H382), Q415 (≠ A405), M417 (= M407), G441 (= G431), D442 (= D432), G443 (= G433), D469 (= D459), G471 (= G461), Y472 (= Y462), N473 (≠ G463), M474 (≠ L464), V475 (≠ I465), Y538 (= Y530)
binding magnesium ion: D442 (= D432), D469 (= D459), G471 (= G461)
binding phosphate ion: G253 (≠ W246), R254 (≠ S247), Q261 (≠ D254), R347 (≠ I341), R398 (= R388), Y401 (≠ H391)

5d6rB Acetolactate synthase from klebsiella pneumoniae in complex with mechanism-based inhibitor
38% identity, 97% coverage: 4:533/547 of query aligns to 8:538/548 of 5d6rB

query
sites
5d6rB

A

A

E

D

L

L

L

V

V

V

Q

S

C

Q

L

L

E

E

N

A

E

Q

G

G

V

V

Q

R

Y

Q

I

V

F

F

G

G

L
x
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

H

K

V

V

L

F

E

D

A

S

L

L

K

L

H

D

S

S

I

I

K

R

F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

R
|
R

L

I

T

T

G

G

K

K

A

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

M

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

L

S

D

E

G

G

A

D

P

P

L

V

V

V

A

A

I

L

T

G

G

G

Q

A

V

V

G

K

T

R

D

A

R

D

M

K

H

A

I

K

E

Q

S

V

H
|
H

Q
|
Q

Y
x
S

L

M

D

D

L

T

V

V

A

A

M

M

F

F

A

S

P

P

V

V

T

T

K

K

W

Y

N

A

K

I

Q

E

I

V

V

T

R

A

P

P

S

D

I

A

T

L

P

A

E

E

V

V

R

S

K

N

A

A

F

F

K

R

R

A

S

A

Q

E

S

Q

E

G

K

R

P

P

G

G

A

S

V

A

H

F

I

V

D

S

L

L

P

P

E
x
Q

N

D

I

V

A

V

A

D

M

G

P

P

V

V

E

S

G

G

K

K

P

V

L

L

R

-

K

P

D

A

N

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M

T

G

Y

A

A

A

S

P

F

D

A

D

S

A

I

I

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D

A

Q

A

V

A

A

A

K

A

L

I

I

C

A

Q

Q

A

A

V

K

N

N

P

P

L

I

I

F

L

L

V

L

G

G

N

L

G

M

A

A

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x
Q

A

P

Q

E

A

N

S

S

D

K

A

A

V

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R

Q

R

F

L

A

L

T

E

L

T

L

S

N

H

I

I

P

P

V

V

A

T

N

S

T

T

F

Y

M

Q

G

A

K

A

G

G

V

A

I

V

P

N

Y

Q

T

D

H

N

-

F

Q

S

L

R

A

F

L

A

W

G

S

R

V

V

G

G

L
|
L

Q

F

Q

N

R

N

D

Q

F

A

I

G

T

D

C

R

G

L

F

L

D

Q

N

L

T

A

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D

L

L

V

V

I

I

A

C

I

I

G

G

Y

Y

D

S

L

P

I

V

E

E

F

Y

S
x
E

P

P

K

A

K

M

W

W

N

N

R

-

N

S

G

G

E

N

I

A

P

T

I

L

V

V

H

H

I

I

G

D

V

V

S

L

P

P

A

A

E

Y

I

E

D

E

S

R

S

N

Y

Y

I

T

P

P

N

D

A

V

E

E

V

L

V

V

G

G

D

D

I

I

S

A

D

G

S

T

L

L

Y

N

E

K

I

L

L

A

K

Q

L

N

A

I

D

D

R

H

Q

R

G

L

K

V

P

L

D

S

P

P

F

Q

A

A

I

A

S

E

L

I

R

L

S

R

D

D

I

R

R

Q

A

H

D

Q

Y

R

E

E

-

L

-

D

-

R

Q

R

Y

G

A

A

H

Q

D

L

D

N

G

Q

F

F

P

A

I

L

K

H

P

P

Q

L

K

R

L

I

I

V

Y

R

D

A

L

M

R

Q

Q

D

V

I

M

V

G

N

P

S

D

D

D

V

I

T

V

L

I

T

S

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D

D

V
x
M

G
|
G

A
x
S

H

F

K

H

M

I

W

W

M

I

A

A

R

R

H

Y

Y

L

H

Y

C

S

H

F

S

R

P

A

N

R

T

Q

C

V

I

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

A

T

M
|
M

G

G

I

V

A

A

I

L

P

P

G

W

A

A

L

I

A

G

A

A

K

W

L

L

V

V

H

N

P

P

K

E

R

R

K

K

V

V

A

S

V

V

T

S

G

G

D
|
D

G
|
G

G

G

F

F

M

L

M

Q

N

S

S

S

Q

M

E

E

L

L

E

E

T

T

A

A

L

V

R

R

V

L

G

K

T

A

P

N

F

V

V

L

T

H

I

L

I

I

F

W

N

V

D
|
D

G

N

G
|
G

Y
|
Y

G
x
N

L
x
M

I
x
V

E

A

W

I

K
x
Q

Q

E

E

E

N

K

H

K

F

Y

G

Q

K

R

G

-

N

-

S

L

S

S

F

G

V

V

H

E

F

F

S

G

N

P

P

M

D

D

F

F

V

K

K

A

Y

Y

A

A

E

E

S

S

M

F

G

G

L

A

K

K

G

G

Y

F

R

A

V

V

E

E

S

S

A

A

L

E

D

A

L

L

I

E

P

P

T

T

L

L

K

R

E

A

A

A

L

M

A

D

Q

V

D

D

V

G

P

P

A

A

V

V

I

V

D

A

C

I

P

P

V

V

D

D

Y
|
Y

R

R

E

D

N

N

active site: I26 (≠ L22), G28 (= G24), A29 (≠ E25), K30 (≠ E26), I31 (≠ N27), E51 (= E47), T74 (= T70), H113 (= H109), Q114 (= Q110), S115 (≠ Y111), Q163 (≠ E159), L254 (= L250), E281 (≠ S277), M386 (≠ V379), Q412 (≠ A405), M414 (= M407), D439 (= D432), D466 (= D459), G468 (= G461), Y469 (= Y462), M471 (≠ L464), V472 (≠ I465), Q475 (≠ K468), Y535 (= Y530)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(Z)-2-fluoro-1-hydroxy-2-phosphonoethenyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: M386 (≠ V379), G387 (= G380), S388 (≠ A381), Q412 (≠ A405), M414 (= M407), D439 (= D432), G440 (= G433), G468 (= G461), Y469 (= Y462), N470 (≠ G463), M471 (≠ L464), Y535 (= Y530)
binding magnesium ion: R63 (= R59), Q212 (≠ R209), D439 (= D432), D466 (= D459), G468 (= G461)

1ozfA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors (see paper)
38% identity, 97% coverage: 4:533/547 of query aligns to 8:537/545 of 1ozfA

query
sites
1ozfA

A

A

E

D

L

L

L

V

V

V

Q

S

C

Q

L

L

E

E

N

A

E

Q

G

G

V

V

Q

R

Y

Q

I

V

F

F

G

G

L
x
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

H

K

V

V

L

F

E

D

A

S

L

L

K

L

H

D

S

S

I

I

K

R

F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

R

R

L

I

T

T

G

G

K

K

A

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

M

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

L

S

D

E

G

G

A

D

P

P

L

V

V

V

A

A

I

L

T

G

G

G

Q

A

V

V

G

K

T

R

D

A

R

D

M

K

H

A

I

K

E

Q

S

V

H
|
H

Q
|
Q

Y
x
S

L

M

D

D

L

T

V

V

A

A

M

M

F

F

A

S

P

P

V

V

T

T

K

K

W

Y

N

A

K

I

Q

E

I

V

V

T

R

A

P

P

S

D

I

A

T

L

P

A

E

E

V

V

R

S

K

N

A

A

F

F

K

R

R

A

S

A

Q

E

S

Q

E

G

K

R

P

P

G

G

A

S

V

A

H

F

I

V

D

S

L

L

P

P

E
x
Q

N

D

I

V

A

V

A

D

M

G

P

P

V

V

E

S

G

G

K

K

P

V

L

L

R

P

K

A

D

-

N

-

S

P

E

Q

K

M

T

G

Y

A

A

A

S

P

F

D

A

D

S

A

I

I

R

D

A

Q

A

V

A

A

A

K

A

L

I

I

C

A

Q

Q

A

A

V

K

N

N

P

P

L

I

I

F

L

L

V

L

G

G

N

L

G

M

A

A

I

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R

Q

A

P

Q

E

A

N

S

S

D

K

A

A

V

L

T

R

Q

R

F

L

A

L

T

E

L

T

L

S

N

H

I

I

P

P

V

V

A

T

N

S

T

T

F

Y

M

Q

G

A

K

A

G

G

V

A

I

V

P

N

Y

Q

T

D

H

N

-

F

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S

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R

A

F

L

A

W
x
G

S
x
R

V

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G

L
|
L

Q

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N

R

N

D
x
Q

F

A

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R

G

L

F

L

D

Q

N

L

T

A

D

D

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L

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V

I

I

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C

I

I

G

G

Y

Y

D

S

L

P

I

V

E

E

F

Y

S
x
E

P

P

K

A

K

M

W

W

N

N

R

-

N

S

G

G

E

N

I

A

P

T

I

L

V

V

H

H

I

I

G

D

V

V

S

L

P

P

A

A

E

Y

I

E

D

E

S

R

S

N

Y

Y

I

T

P

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N

D

A

V

E

E

V

L

V

V

G

G

D

D

I

I

S

A

D

G

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T

L

L

Y

N

E

K

I

L

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A

K

Q

L

N

A

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Q

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G

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P

P

F

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A

A

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A

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E

L

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R

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I
x
R

R

Q

A

H

D

Q

Y

R

E

E

-

L

-

D

-

R

Q

R

Y

G

A

A

H

Q

D

L

D

N

G

Q

F

F

P

A

I

L

K

H

P

P

Q

L

K

R

L

I

I

V

Y

R

D

A

L

M

R

Q

Q

D

V

I

M

V

G

N

P

S

D

D

D

V

I

T

V

L

I

T

S

V

D

D

V
x
M

G
|
G

A
x
S

H
x
F

K

H

M

I

W

W

M

I

A

A

R
|
R

H

Y

Y
x
L

H
x
Y

C

T

H

F

S

R

P

A

N

R

T

Q

C

V

I

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

A

T

M
|
M

G

G

I

V

A

A

I

L

P

P

G

W

A

A

L

I

A

G

A

A

K

W

L

L

V

V

H

N

P

P

K

E

R

R

K

K

V

V

A

S

V

V

T

S

G
|
G

D
|
D

G
|
G

G

G

F

F

M

L

M

Q

N

S

S

S

Q

M

E

E

L

L

E

E

T

T

A

A

L

V

R

R

V

L

G

K

T

A

P

N

F

V

V

L

T

H

I

L

I

I

F

W

N

V

D
|
D

G

N

G
|
G

Y
|
Y

G
x
N

L
x
M

I
x
V

E

A

W

I

K
x
Q

Q

E

E

E

N

K

H

K

F

Y

G

Q

K

R

G

-

N

-

S

L

S

S

F

G

V

V

H

E

F

F

S

G

N

P

P

M

D

D

F

F

V

K

K

A

Y

Y

A

A

E

E

S

S

M

F

G

G

L

A

K

K

G

G

Y

F

R

A

V

V

E

E

S

S

A

A

L

E

D

A

L

L

I

E

P

P

T

T

L

L

K

R

E

A

A

A

L

M

A

D

Q

V

D

D

V

G

P

P

A

A

V

V

I

V

D

A

C

I

P

P

V

V

D

D

Y
|
Y

R

R

E

D

N

N

active site: I26 (≠ L22), G28 (= G24), A29 (≠ E25), K30 (≠ E26), I31 (≠ N27), E51 (= E47), T74 (= T70), H113 (= H109), Q114 (= Q110), S115 (≠ Y111), Q163 (≠ E159), L253 (= L250), E280 (≠ S277), M385 (≠ V379), Q411 (≠ A405), M413 (= M407), D438 (= D432), D465 (= D459), G467 (= G461), Y468 (= Y462), M470 (≠ L464), V471 (≠ I465), Q474 (≠ K468), Y534 (= Y530)
binding magnesium ion: D438 (= D432), D465 (= D459), G467 (= G461)
binding phosphate ion: G249 (≠ W246), R250 (≠ S247), Q257 (≠ D254), R343 (≠ I341), R394 (= R388), L396 (≠ Y390), Y397 (≠ H391)
binding thiamine diphosphate: G386 (= G380), S387 (≠ A381), F388 (≠ H382), Q411 (≠ A405), M413 (= M407), G437 (= G431), D438 (= D432), G439 (= G433), D465 (= D459), G467 (= G461), Y468 (= Y462), N469 (≠ G463), M470 (≠ L464), V471 (≠ I465), Y534 (= Y530)

5wdgA Acetolactate synthase from klebsiella pneumoniae in complex with a reaction intermediate
38% identity, 97% coverage: 4:533/547 of query aligns to 9:530/538 of 5wdgA

query
sites
5wdgA

A

A

E

D

L

L

L

V

V

V

Q

S

C

Q

L

L

E

E

N

A

E

Q

G

G

V

V

Q

R

Y

Q

I

V

F

F

G

G

L
x
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

H

K

V

V

L

F

E

D

A

S

L

L

K

L

H

D

S

S

I

I

K

R

F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

R
|
R

L

I

T

T

G

G

K

K

A

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

M

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

L

S

D

E

G
|
G

A

D

P

P

L

V

V

V

A

A

I

L

T

G

G

G

Q

A

V

V

G

K

-

R

T

A

D

D

R

K

M

M

H

-

I

-

E

-

S

-

H

-

Q

-

Y

-

L

-

D

D

L

T

V

V

A

A

M

M

F

F

A
x
S

P

P

V

V

T
|
T

K

K

W

Y

N

A

K

I

Q

E

I

V

V

T

R

A

P

P

S

D

I

A

T

L

P

A

E

E

V

V

R

S

K

N

A

A

F

F

K

R

R

A

S

A

Q

E

S

Q

E

G

K
x
R

P

P

G

G

A

S

V

A

H

F

I

V

D

S

L

L

P

P

E
x
Q

N

D

I

V

A

V

A

D

M

G

P

P

V

V

E

S

G

G

K

K

P

V

L

L

R

-

K

-

D

-

N

P

S

A

E

Q

K

M

T

G

Y

A

A

A

S

P

F

D

A

D

S

A

I

I

R

D

A

Q

A

V

A

A

A

K

A

L

I

I

C

A

Q

Q

A

A

V

K

N

N

P

P

L

I

I

F

L

L

V

L

G

G

N

L

G

M

A

A

I

S

R
x
Q

A

P

Q

E

A

N

S

S

D

K

A

A

V

L

T

R

Q

R

F

L

A

L

T

E

L

T

L

S

N

H

I

I

P

P

V

V

A

T

N

S

T

T

F

Y

M

Q

G

A

K

A

G

G

V

A

I

V

P

N

Y

Q

T

D

H

N

-

F

Q

S

L

R

A

F

L

A

W

G

S

R

V

V

G

G

L
|
L

Q

F

Q

N

R

N

D

Q

F

A

I

G

T

D

C

R

G

L

F

L

D

Q

N

L

T

A

D

D

L

L

V

V

I

I

A

C

I

I

G

G

Y

Y

D

S

L

P

I

V

E

E

F

Y

S
x
E

P

P

K

A

K

M

W

W

N

N

R

-

N

S

G

G

E

N

I

A

P

T

I

L

V

V

H

H

I

I

G

D

V

V

S

L

P

P

A

A

E

Y

I

E

D

E

S

R

S

N

Y

Y

I

T

P

P

N

D

A

V

E

E

V

L

V

V

G

G

D

D

I

I

S

A

D

G

S

T

L

L

Y

N

E

K

I

L

L

A

K

Q

L

N

A

I

D

D

R

H

Q

R

G

L

K

V

P

L

D

S

P

P

F

Q

A

A

I

A

S

E

L

I

R

L

S

R

D

D

I

R

R

Q

A

H

D

Q

Y

R

E

E

-

L

-

D

-

R

Q

R

Y

G

A

A

H

Q

D

L

D

N

G

Q

F

F

P

A

I

L

K

H

P

P

Q

L

K

R

L

I

I

V

Y

R

D

A

L

M

R

Q

Q

D

V

I

M

V

G

N

P

S

D

D

D

V

I

T

V

L

I

T

S

V

D

D

V
x
M

G

G

A
x
S

H
x
F

K

H

M

I

W

W

M

I

A

A

R

R

H

Y

Y

L

H

Y

C

S

H

F

S

R

P

A

N

R

T

Q

C

V

I

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

A

T

M
|
M

G

G

I

V

A

A

I

L

P

P

G

W

A

A

L

I

A

G

A

A

K

W

L

L

V

V

H

N

P

P

K

E

R

R

K

K

V

V

A

S

V

V

T

S