SitesBLAST
Comparing WP_012412124.1 NCBI__GCF_000020025.1:WP_012412124.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5mhaB D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
32% identity, 78% coverage: 65:309/315 of query aligns to 61:307/308 of 5mhaB
- binding 2-Ketohexanoic acid: R66 (≠ N70), R226 (= R228), H274 (= H276), Y282 (≠ I284)
- binding (2R)-2-hydroxyhexanoic acid: R66 (≠ N70), A67 (= A71), G68 (= G72), H91 (= H95), Y282 (≠ I284)
- binding magnesium ion: F212 (≠ L214), E213 (≠ R215), M215 (≠ L217), D243 (≠ W245)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (= A71), G68 (= G72), T88 (≠ A92), L143 (≠ A145), G144 (= G146), T145 (≠ G147), L146 (≠ I148), R165 (= R167), R166 (= R168), S167 (≠ H169), P180 (≠ G182), T197 (= T199), P198 (= P200), T203 (= T205), V224 (≠ I226), A225 (= A227), R226 (= R228), H274 (= H276), S276 (= S278)
5mhaA D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
32% identity, 78% coverage: 65:309/315 of query aligns to 61:307/308 of 5mhaA
- binding 2-Ketohexanoic acid: R66 (≠ N70), A67 (= A71), G68 (= G72), H91 (= H95), R226 (= R228), H274 (= H276), Y282 (≠ I284)
- binding magnesium ion: T132 (≠ E134), A134 (≠ T136)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (= A71), G68 (= G72), T88 (≠ A92), L143 (≠ A145), G144 (= G146), T145 (≠ G147), L146 (≠ I148), R165 (= R167), R166 (= R168), S167 (≠ H169), P180 (≠ G182), T197 (= T199), P198 (= P200), T203 (= T205), V224 (≠ I226), A225 (= A227), R226 (= R228), H274 (= H276), S276 (= S278)
5mh5A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-keto-hexanoic acid and NADP+ (1.4 a resolution)
32% identity, 78% coverage: 65:309/315 of query aligns to 61:307/308 of 5mh5A
- binding 2-Ketohexanoic acid: R66 (≠ N70), A67 (= A71), G68 (= G72), H91 (= H95), R226 (= R228), H274 (= H276), Y282 (≠ I284)
- binding magnesium ion: T132 (≠ E134), A134 (≠ T136), F212 (≠ L214), E213 (≠ R215), M215 (≠ L217), D243 (≠ W245)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: A67 (= A71), G68 (= G72), T88 (≠ A92), G142 (= G144), L143 (≠ A145), G144 (= G146), T145 (≠ G147), L146 (≠ I148), R165 (= R167), R166 (= R168), S167 (≠ H169), T197 (= T199), P198 (= P200), T203 (= T205), V224 (≠ I226), A225 (= A227), R226 (= R228), H274 (= H276), S276 (= S278)
5mh6A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-ketohexanoic acid and NAD+ (1.35 a resolution)
32% identity, 78% coverage: 65:309/315 of query aligns to 59:305/306 of 5mh6A
- binding 2-Ketohexanoic acid: R64 (≠ N70), A65 (= A71), G66 (= G72), H89 (= H95), R224 (= R228), H272 (= H276), Y280 (≠ I284)
- binding magnesium ion: T130 (≠ E134), A132 (≠ T136), F210 (≠ L214), E211 (≠ R215), M213 (≠ L217), G225 (= G229), P226 (≠ G230), V228 (≠ I232), E230 (= E234), D241 (≠ W245), S251 (≠ N255)
- binding nicotinamide-adenine-dinucleotide: A65 (= A71), G66 (= G72), T86 (≠ A92), H89 (= H95), G142 (= G146), T143 (≠ G147), L144 (≠ I148), R164 (= R168), P196 (= P200), T201 (= T205), V222 (≠ I226), A223 (= A227), R224 (= R228), H272 (= H276), S274 (= S278)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
37% identity, 83% coverage: 52:312/315 of query aligns to 57:324/334 of 5aovA
- active site: L100 (≠ H95), R241 (= R228), D265 (= D252), E270 (= E257), H288 (= H276)
- binding glyoxylic acid: Y74 (≠ P69), A75 (≠ N70), V76 (≠ A71), G77 (= G72), R241 (= R228), H288 (= H276)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A71), T104 (≠ I99), F158 (≠ A145), G159 (= G146), R160 (≠ G147), I161 (= I148), S180 (≠ R167), R181 (= R168), A211 (= A198), V212 (≠ T199), P213 (= P200), T218 (= T205), I239 (= I226), A240 (= A227), R241 (= R228), H288 (= H276), G290 (≠ S278)
Sites not aligning to the query:
5vg6B Crystal structure of d-isomer specific 2-hydroxyacid dehydrogenase from xanthobacter autotrophicus py2 in complex with NADPH and mes.
32% identity, 91% coverage: 29:315/315 of query aligns to 37:315/315 of 5vg6B
- active site: M98 (vs. gap), R230 (= R228), D254 (= D252), E259 (= E257), H278 (= H276)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A74 (= A71), R92 (≠ N90), M102 (≠ I99), L147 (≠ A145), G148 (= G146), D149 (≠ G147), L150 (≠ I148), W168 (≠ S166), S169 (≠ R167), R170 (= R168), T171 (≠ H169), K173 (≠ Q171), L201 (≠ T199), P202 (= P200), T207 (= T205), V228 (≠ I226), R230 (= R228), H278 (= H276), A280 (≠ S278), S281 (= S279), Y315 (= Y315)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
35% identity, 83% coverage: 52:312/315 of query aligns to 56:323/332 of 6biiA
- active site: L99 (≠ H95), R240 (= R228), D264 (= D252), E269 (= E257), H287 (= H276)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ A71), T103 (≠ I99), G156 (= G144), F157 (≠ A145), G158 (= G146), R159 (≠ G147), I160 (= I148), A179 (≠ R167), R180 (= R168), S181 (≠ H169), K183 (≠ Q171), V211 (≠ T199), P212 (= P200), E216 (= E204), T217 (= T205), V238 (≠ I226), A239 (= A227), R240 (= R228), D264 (= D252), H287 (= H276), G289 (≠ S278)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
28% identity, 84% coverage: 50:313/315 of query aligns to 57:320/533 of O43175
- T78 (≠ A71) binding NAD(+)
- R135 (= R128) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ GI 147:148) binding NAD(+)
- D175 (≠ H169) binding NAD(+)
- T207 (= T199) binding NAD(+)
- CAR 234:236 (≠ IAR 226:228) binding NAD(+)
- D260 (= D252) binding NAD(+)
- V261 (≠ T253) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HISS 276:279) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
28% identity, 79% coverage: 50:297/315 of query aligns to 53:304/305 of 6plfA
7dkmA Phgdh covalently linked to oridonin (see paper)
28% identity, 79% coverage: 50:297/315 of query aligns to 53:304/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ A71), A102 (≠ I99), G148 (= G144), R151 (≠ G147), I152 (= I148), Y170 (≠ S166), D171 (≠ H169), P172 (= P170), I173 (≠ Q171), H202 (≠ A198), T203 (= T199), P204 (= P200), T209 (= T205), C230 (≠ I226), A231 (= A227), R232 (= R228), H279 (= H276), G281 (≠ S278)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: E293 (vs. gap)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
28% identity, 79% coverage: 50:297/315 of query aligns to 52:303/303 of 6plgA
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
28% identity, 78% coverage: 50:294/315 of query aligns to 49:297/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G146), I148 (= I148), Y166 (≠ R168), D167 (≠ H169), P168 (= P170), I169 (≠ Q171), I170 (≠ E172), H198 (≠ A198), T199 (= T199), L208 (≠ F208), R228 (= R228)
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
28% identity, 78% coverage: 50:294/315 of query aligns to 52:300/301 of 6rj5A
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
28% identity, 78% coverage: 50:294/315 of query aligns to 52:300/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I143), G147 (= G144), L148 (≠ A145), G149 (= G146), R150 (≠ G147), I151 (= I148), G152 (= G149), D170 (≠ H169), H201 (≠ A198), T202 (= T199), P203 (= P200)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
28% identity, 78% coverage: 50:294/315 of query aligns to 52:300/302 of 6rihA
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
28% identity, 78% coverage: 50:294/315 of query aligns to 51:299/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (≠ H95), A100 (≠ I99), R149 (≠ G147), I150 (= I148), Y168 (≠ S166), D169 (≠ H169), P170 (= P170), I171 (≠ Q171), H200 (≠ A198), T201 (= T199), P202 (= P200), T207 (= T205), C228 (≠ I226), A229 (= A227), R230 (= R228), H277 (= H276), G279 (≠ S278)
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
29% identity, 76% coverage: 56:294/315 of query aligns to 49:291/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (≠ G147), Y160 (≠ S166), D161 (≠ H169), P162 (= P170), I164 (≠ E172), L179 (vs. gap), T193 (= T199), P194 (= P200), S198 (≠ E204), L202 (≠ F208)
4z0pA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc02828 (smghra) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
29% identity, 83% coverage: 56:315/315 of query aligns to 56:316/316 of 4z0pA
- active site: L95 (= L88), R231 (= R228), G250 (≠ A247), D255 (= D252), E260 (= E257), H279 (= H276)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R89 (≠ L82), M99 (≠ I99), M144 (≠ A145), G145 (= G146), V146 (≠ G147), L147 (≠ I148), W165 (vs. gap), S166 (vs. gap), R167 (vs. gap), S168 (= S166), R170 (= R168), L197 (≠ T199), P198 (= P200), A229 (≠ I226), G230 (≠ A227), R231 (= R228), H279 (= H276), A281 (≠ S278), A282 (≠ S279), Y316 (= Y315)
- binding oxalic acid: G70 (≠ N70), A71 (= A71), G72 (= G72), H114 (≠ L114), R115 (≠ P115), R231 (= R228), H279 (= H276)
Sites not aligning to the query:
4weqA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc02828 (smghra) from sinorhizobium meliloti in complex with NADP and sulfate (see paper)
29% identity, 83% coverage: 56:315/315 of query aligns to 56:316/316 of 4weqA
- active site: L95 (= L88), R231 (= R228), G250 (≠ A247), D255 (= D252), E260 (= E257), H279 (= H276)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R89 (≠ L82), M99 (≠ I99), M144 (≠ A145), G145 (= G146), V146 (≠ G147), L147 (≠ I148), W165 (vs. gap), S166 (vs. gap), R167 (vs. gap), S168 (= S166), R170 (= R168), L197 (≠ T199), P198 (= P200), A229 (≠ I226), G230 (≠ A227), R231 (= R228), D255 (= D252), H279 (= H276), A281 (≠ S278), Y316 (= Y315)
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
28% identity, 76% coverage: 50:288/315 of query aligns to 51:289/297 of 6rj3A
Query Sequence
>WP_012412124.1 NCBI__GCF_000020025.1:WP_012412124.1
MKLILPLDLVADIEPHLPPDTEIVTVDVEGNLDGDATDAEVYFSWFLARSPILHKIIAAA
PALRWHHAPNAGVNHILTPTYLERDIILTNGAGVHGIPIAEFAIAYILAHAKHLPELYAL
QAERHWKRGFAIQELTDATLLIIGAGGIGQEIAARAKPFGLRIIGSRRHPQELPNFDKVV
GGDEWRALLPGVNYVVIATPLTPETKEFIDESVLRLLPKHAYLINIARGGVIDELALIKA
LTEGWIAGEALDTVNSEPLPSESPLWSLPNIFITPHISSDSPKIKQRSIVLFIDNLKRYQ
AGQPLRNVVDKEAGY
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory