SitesBLAST
Comparing WP_012465441.1 NCBI__GCF_000020465.1:WP_012465441.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1bxrA Structure of carbamoyl phosphate synthetase complexed with the atp analog amppnp (see paper)
63% identity, 99% coverage: 1:488/495 of query aligns to 1:479/1073 of 1bxrA
- active site: R129 (= R129), R169 (= R169), M174 (≠ L174), G176 (= G176), K202 (≠ S202), E215 (= E215), H243 (= H243), N283 (= N283), Q285 (= Q285), E299 (= E299), N301 (= N301), R303 (= R303), S307 (= S307), D338 (= D338)
- binding phosphoaminophosphonic acid-adenylate ester: R129 (= R129), I167 (≠ V167), R169 (= R169), M174 (≠ L174), G175 (= G175), G176 (= G176), L210 (= L210), I211 (≠ V211), E215 (= E215), M240 (= M240), G241 (= G241), H243 (= H243), T244 (= T244), Q285 (= Q285), E299 (= E299), R306 (= R306), T376 (≠ V375)
- binding manganese (ii) ion: E299 (= E299), N301 (= N301)
Sites not aligning to the query:
- active site: 507, 634, 715, 721, 722, 745, 761, 769, 829, 841, 843, 848, 901
- binding phosphoaminophosphonic acid-adenylate ester: 675, 713, 715, 720, 721, 722, 725, 753, 755, 756, 761, 785, 786, 787, 788, 829, 841, 843, 848
- binding manganese (ii) ion: 829, 841, 841, 843
- binding L-ornithine: 783, 791, 892, 907, 1041, 1042
P00968 Carbamoyl phosphate synthase large chain; Carbamoyl phosphate synthetase ammonia chain; EC 6.3.4.16; EC 6.3.5.5 from Escherichia coli (strain K12) (see 6 papers)
63% identity, 99% coverage: 1:488/495 of query aligns to 1:479/1073 of P00968
- M1 (= M1) modified: Initiator methionine, Removed
- R129 (= R129) binding ATP
- R169 (= R169) binding ATP
- G175 (= G175) binding ATP
- G176 (= G176) binding ATP
- E208 (= E208) binding ATP
- L210 (= L210) binding ATP
- E215 (= E215) binding ATP
- G241 (= G241) binding ATP
- I242 (≠ V242) binding ATP
- H243 (= H243) binding ATP
- Q285 (= Q285) binding ATP; binding Mn(2+)
- E299 (= E299) binding ATP; binding Mn(2+); binding Mn(2+)
- N301 (= N301) binding Mn(2+)
Sites not aligning to the query:
- 715 binding ATP
- 754 binding ATP
- 756 binding ATP
- 761 binding ATP
- 786 binding ATP
- 787 binding ATP
- 788 binding ATP
- 789 binding ATP
- 829 binding ATP; binding Mn(2+)
- 841 binding ATP; binding Mn(2+); binding Mn(2+)
- 843 binding Mn(2+)
1t36A Crystal structure of e. Coli carbamoyl phosphate synthetase small subunit mutant c248d complexed with uridine 5'-monophosphate (see paper)
63% identity, 99% coverage: 1:488/495 of query aligns to 1:479/1058 of 1t36A
- active site: R129 (= R129), R169 (= R169), M174 (≠ L174), G176 (= G176), K202 (≠ S202), E215 (= E215), H243 (= H243), N283 (= N283), Q285 (= Q285), E299 (= E299), N301 (= N301), R303 (= R303), S307 (= S307), D338 (= D338)
- binding adenosine-5'-diphosphate: R129 (= R129), I167 (≠ V167), R169 (= R169), M174 (≠ L174), G175 (= G175), G176 (= G176), E208 (= E208), L210 (= L210), I211 (≠ V211), E215 (= E215), M240 (= M240), G241 (= G241), I242 (≠ V242), H243 (= H243), Q285 (= Q285), I298 (= I298), E299 (= E299), T376 (≠ V375)
- binding manganese (ii) ion: Q285 (= Q285), E299 (= E299), E299 (= E299), N301 (= N301)
- binding phosphate ion: M174 (≠ L174), G175 (= G175), H243 (= H243), E299 (= E299), N301 (= N301), R303 (= R303), R306 (= R306)
Sites not aligning to the query:
- active site: 507, 634, 715, 746, 754, 814, 826, 828, 833, 886
- binding adenosine-5'-diphosphate: 715, 718, 740, 741, 746, 770, 771, 772, 773, 826, 894
- binding manganese (ii) ion: 814, 826
- binding L-ornithine: 768, 776, 877, 892, 1026, 1027
- binding uridine-5'-monophosphate: 939, 959, 961, 962, 978, 1000, 1001, 1002, 1011, 1014
1c3oA Crystal structure of the carbamoyl phosphate synthetase: small subunit mutant c269s with bound glutamine (see paper)
63% identity, 99% coverage: 1:488/495 of query aligns to 1:479/1058 of 1c3oA
- active site: R129 (= R129), R169 (= R169), M174 (≠ L174), G176 (= G176), K202 (≠ S202), E215 (= E215), H243 (= H243), N283 (= N283), Q285 (= Q285), E299 (= E299), N301 (= N301), R303 (= R303), S307 (= S307), D338 (= D338)
- binding adenosine-5'-diphosphate: R129 (= R129), I167 (≠ V167), R169 (= R169), M174 (≠ L174), G176 (= G176), L210 (= L210), I211 (≠ V211), E215 (= E215), M240 (= M240), G241 (= G241), H243 (= H243), T244 (= T244), Q285 (= Q285), I298 (= I298), E299 (= E299), T376 (≠ V375)
- binding manganese (ii) ion: Q285 (= Q285), E299 (= E299), E299 (= E299), N301 (= N301)
- binding phosphate ion: M174 (≠ L174), G175 (= G175), H243 (= H243), E299 (= E299), N301 (= N301), R303 (= R303), R306 (= R306)
Sites not aligning to the query:
- active site: 507, 634, 715, 746, 754, 814, 826, 828, 833, 886
- binding adenosine-5'-diphosphate: 715, 718, 740, 741, 746, 770, 771, 772, 773, 774, 826
- binding glutamine: 528, 537, 538, 554
- binding manganese (ii) ion: 814, 826
- binding L-ornithine: 768, 776, 877, 892, 1026, 1027
1a9xA Carbamoyl phosphate synthetase: caught in the act of glutamine hydrolysis (see paper)
63% identity, 99% coverage: 1:488/495 of query aligns to 1:479/1058 of 1a9xA
- active site: K202 (≠ S202), D338 (= D338)
- binding adenosine-5'-diphosphate: R129 (= R129), I167 (≠ V167), R169 (= R169), M174 (≠ L174), G175 (= G175), G176 (= G176), L210 (= L210), E215 (= E215), M240 (= M240), G241 (= G241), I242 (≠ V242), H243 (= H243), T244 (= T244), Q285 (= Q285), I298 (= I298), E299 (= E299), T376 (≠ V375)
- binding manganese (ii) ion: Q285 (= Q285), E299 (= E299), E299 (= E299), N301 (= N301)
- binding phosphate ion: G175 (= G175), H243 (= H243), E299 (= E299), N301 (= N301), R303 (= R303), R306 (= R306)
Sites not aligning to the query:
- active site: 507, 634, 754, 886
- binding adenosine-5'-diphosphate: 715, 718, 740, 741, 746, 770, 771, 772, 773, 826
- binding manganese (ii) ion: 814, 826
- binding L-ornithine: 768, 776, 877, 892, 1025, 1026, 1027
1ce8A Carbamoyl phosphate synthetase from escherichis coli with complexed with the allosteric ligand imp (see paper)
63% identity, 99% coverage: 1:488/495 of query aligns to 1:479/1058 of 1ce8A
- active site: R129 (= R129), R169 (= R169), M174 (≠ L174), G176 (= G176), K202 (≠ S202), E215 (= E215), H243 (= H243), N283 (= N283), Q285 (= Q285), E299 (= E299), N301 (= N301), R303 (= R303), S307 (= S307), D338 (= D338)
- binding adenosine-5'-diphosphate: R129 (= R129), I167 (≠ V167), R169 (= R169), M174 (≠ L174), G176 (= G176), L210 (= L210), I211 (≠ V211), E215 (= E215), M240 (= M240), G241 (= G241), I242 (≠ V242), H243 (= H243), Q285 (= Q285), I298 (= I298), E299 (= E299), T376 (≠ V375)
- binding manganese (ii) ion: M174 (≠ L174), Q285 (= Q285), E299 (= E299), E299 (= E299), N301 (= N301)
- binding phosphate ion: M174 (≠ L174), G175 (= G175), H243 (= H243), E299 (= E299), N301 (= N301), R303 (= R303), R306 (= R306)
Sites not aligning to the query:
- active site: 507, 634, 715, 746, 754, 814, 826, 828, 833, 886
- binding adenosine-5'-diphosphate: 715, 740, 741, 746, 770, 771, 772, 773, 774, 826
- binding inosinic acid: 933, 939, 959, 961, 962, 978, 979, 986, 1000, 1001, 1002, 1010, 1011, 1013
- binding manganese (ii) ion: 814, 826
- binding L-ornithine: 528, 537, 538, 552, 554, 768, 776, 877, 892, 1025, 1026, 1027
Q42601 Carbamoyl phosphate synthase arginine-specific large chain, chloroplastic; CPS; CPSase; Ammonium-dependent carbamoyl phosphate synthetase; Arginine-specific carbamoyl phosphate synthetase, ammonia chain; Glutamine-dependent carbamoyl phosphate synthetase; Protein VENOSA 3; EC 6.3.4.16; EC 6.3.5.5 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
62% identity, 98% coverage: 3:485/495 of query aligns to 94:567/1187 of Q42601
- P149 (≠ I58) mutation to L: In ven3-2; reduced plant size and reticulate leaf phenotype.
Sites not aligning to the query:
- 587 G→E: In ven3-3; reticulate leaf phenotype.
- 844 A→T: In ven3-4; reduced plant size and reticulate leaf phenotype.
- 1014 P→L: In ven3-1; reticulate leaf phenotype.
P03965 Carbamoyl phosphate synthase arginine-specific large chain; CPS; CPSase; CPSase-arg; Ammonium-dependent carbamoyl phosphate synthetase; Arginine-specific carbamoyl phosphate synthetase, ammonia chain; Carbamoyl phosphate synthase A; CPS-A; Glutamine-dependent carbamoyl phosphate synthetase; EC 6.3.4.16; EC 6.3.5.5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
46% identity, 98% coverage: 5:491/495 of query aligns to 26:495/1118 of P03965
- L229 (= L210) mutation to G: Abolishes ammonia-dependent ATPase activity.
- H262 (= H243) mutation to N: No effect.
- D265 (= D246) mutation D->A,E,N: Reduces ammonia-dependent ATPase activity 17-58 fold.
- I316 (= I298) mutation I->G,S,H: Reduces ammonia-dependent ATPase activity 17-64 fold.
Sites not aligning to the query:
- 807 H→N: No effect.
- 810 mutation D->A,E,N: Abolishes ammonia-dependent ATPase activity.
P05990 Multifunctional protein r; Protein rudimentary; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Drosophila melanogaster (Fruit fly) (see 2 papers)
44% identity, 98% coverage: 1:485/495 of query aligns to 402:865/2224 of P05990