SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012466757.1 NCBI__GCF_000020465.1:WP_012466757.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
42% identity, 72% coverage: 44:161/164 of query aligns to 115:232/233 of 4n6bA

query
sites
4n6bA

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binding coenzyme a: H150 (= H79), L169 (≠ F98), G176 (≠ A105), K208 (≠ R137), G210 (= G139), A211 (= A140), L216 (≠ I145), V226 (= V155)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
40% identity, 73% coverage: 43:161/164 of query aligns to 125:246/272 of 3gvdI

query
sites
3gvdI

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binding cysteine: D160 (≠ P78), G186 (= G104), G187 (≠ A105), R195 (vs. gap)

Sites not aligning to the query:

binding cysteine: 95, 96

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
42% identity, 70% coverage: 47:160/164 of query aligns to 127:243/261 of 6wyeA

query
sites
6wyeA

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binding (2S)-2-hydroxybutanedioic acid: D158 (≠ P78), H159 (= H79), G185 (≠ A105), R193 (≠ E110), H194 (≠ V111)

Sites not aligning to the query:

binding (2S)-2-hydroxybutanedioic acid: 93, 94

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
42% identity, 70% coverage: 47:160/164 of query aligns to 125:241/243 of 7ra4A

query
sites
7ra4A

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binding serine: D156 (≠ P78), H157 (= H79), R191 (≠ E110), H192 (≠ V111)

Sites not aligning to the query:

binding serine: 91, 92

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
39% identity, 73% coverage: 43:161/164 of query aligns to 118:239/257 of 1ssqD

query
sites
1ssqD

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binding cysteine: D153 (≠ P78), H154 (= H79)

Sites not aligning to the query:

binding cysteine: 88

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
40% identity, 72% coverage: 44:161/164 of query aligns to 122:242/243 of 4n69A

query
sites
4n69A

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binding serine: G183 (≠ A105), R191 (≠ E110), H192 (≠ V111)

1sstA Serine acetyltransferase- complex with coa (see paper)
39% identity, 73% coverage: 43:161/164 of query aligns to 118:232/233 of 1sstA

query
sites
1sstA

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binding coenzyme a: H154 (= H79), L173 (≠ F98), G180 (≠ A105), T181 (≠ S106), A193 (= A122), L198 (= L127), K208 (≠ R137), G210 (= G139), A211 (= A140), V214 (= V143), L216 (≠ I145), T224 (= T153), A226 (≠ V155), V228 (≠ I157), P229 (= P158)

Sites not aligning to the query:

binding coenzyme a: 233

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
38% identity, 70% coverage: 47:161/164 of query aligns to 122:239/258 of 4h7oA

query
sites
4h7oA

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binding arginine: Y127 (≠ L52), Q131 (≠ F56)
binding cysteine: D153 (≠ P78), H154 (= H79), G180 (≠ A105), R188 (≠ E110), H189 (≠ V111)

Sites not aligning to the query:

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
39% identity, 73% coverage: 43:161/164 of query aligns to 122:243/262 of 1t3dA

query
sites
1t3dA

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binding cysteine: D157 (≠ P78), G184 (≠ A105), R192 (≠ E110), H193 (≠ V111)

Sites not aligning to the query:

binding cysteine: 92, 93

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
31% identity, 98% coverage: 1:161/164 of query aligns to 70:243/250 of 4hzdA

query
sites
4hzdA

M

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L

M

Y

L

L

A

K

A

G

L

F

R

H

R

A

Y

I

Q

Q

K

T

N

H

S

R

R

L

K

A

A

H

D

W

P

L

V

Y

S

K

Q

Q

T

G

I

R

K

K

R

D

I

F

A

A

V

Y

L

Y

Q

L

H

Q

R

S

F

R

W

S

S

S

A

S

V

I

C

F

G

Q

A

T

D

D

I

I

P

H

L

P

D

A

S

A

N

R

I

L

G

G

G

S

G

G

L

L

L

F

I

L

P

D

H
|
H

P

A

N

T

G

G

I

L

V

V

I

V

H

G

P

E

Q

T

A

A

K

V

I

V

G

E

P

D

N

N

C

V

L

S

I

I

F

L

Q

H

Q

G

V

V

T

T

I

L

G

G

A

G

S

T

G

G

A

K

S

S

E

S

-

G

-

D

-

R

V

H

P

P

V

K

I

I

E

R

G

Q

H

G

V

V

D

L

I

I

G
|
G

A
|
A

G

G

A

A

R

K

I

I

L

L

G

G

N

N

I

I

T

Q

I

V

G

G

A

Q

H

C

A

S

R

K

I

I

G
x
A

A
|
A

N

G

A
x
S

V

V

V

V

I

L

T

K

D

S

I

V

P

P

A

H

N

N

A

V

T
|
T

A

V

V
x
A

G
|
G

I

V

P

P

G

A

K

R

I

I

binding coenzyme a: H158 (= H79), G203 (= G121), A204 (= A122), A221 (≠ G139), A222 (= A140), T235 (= T153), A237 (≠ V155)
binding magnesium ion: S224 (≠ A142), A237 (≠ V155), G238 (= G156)

Sites not aligning to the query:

binding coenzyme a: 244

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
38% identity, 70% coverage: 47:161/164 of query aligns to 122:239/258 of 8i04A

query
sites
8i04A

K

R

R

A

I

L

A

A

V

I

L

F

Q

L

H

Q

R

N

F

Q

W

V

S

S

A

V

V

S

C

F

G

Q

A

V

D

D

I

I

P

H

L

P

D

A

S

A

N

K

I

I

G

G

G

R

G

G

L

I

L

M

I

L

P
x
D

H
|
H

P

A

N

T

G

G

I

I

V

V

I

V

H

G

P

E

Q

T

A

A

K

V

I

I

G

E

P

D

N

D

C

V

L

S

I

I

F

L

Q

Q

Q

S

V

V

T

T

I

L

G

G

A
x
G

S

T

G

G

A

K

S

T

E

S

-

G

-

D

-
x
R

V
x
H

P

P

V

K

I

I

E

R

G

E

H

G

V

V

D

M

I

I

G

G

A

A

G

G

A

A

R

K

I

I

L

L

G

G

N

N

I

I

T

E

I

V

G

G

A

R

H

G

A

A

R

K

I

I

G

G

A

A

N

G

A

S

V

V

V

V

I

L

T

Q

D

P

I

V

P

P

A

P

N

H

A

T

T

T

A

A

V

A

G

G

I

V

P

P

G

A

K

R

I

I

binding serine: D153 (≠ P78), H154 (= H79), G180 (≠ A105), R188 (vs. gap), H189 (≠ V111)

Sites not aligning to the query:

binding serine: 88, 89

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
38% identity, 70% coverage: 47:161/164 of query aligns to 125:242/246 of 8i09A

query
sites
8i09A

K

R

R

A

I

L

A

A

V

I

L

F

Q

L

H

Q

R

N

F

Q

W

V

S

S

A

V

V

S

C

F

G

Q

A

V

D

D

I

I

P

H

L

P

D

A

S

A

N

K

I

I

G

G

G

R

G

G

L

I

L

M

I

L

P
x
D

H
|
H

P

A

N

T

G

G

I

I

V

V

I

V

H
x
G

P

E

Q

T

A

A

K

V

I

I

G

E

P

D

N

D

C

V

L

S

I

I

F

L

Q

Q

Q

S

V

V

T

T

I

L

G

G

A
x
G

S
x
T

G

G

A

K

S

T

E

S

-

G

-

D

-
x
R

V
x
H

P

P

V

K

I

I

E

R

G

E

H

G

V

V

D

M

I

I

G
|
G

A

A

G

G

A

A

R

K

I

I

L

L

G

G

N

N

I

I

T

E

I

V

G

G

A

R

H

G

A

A

R
x
K

I
|
I

G
|
G

A

A

N

G

A

S

V

V

V

V

I
x
L

T

Q

D

P

I

V

P

P

A

P

N

H

A

T

T

T

A

A

V
x
A

G

G

I

V

P

P

G

A

K

R

I

I

binding cysteine: D156 (≠ P78), H157 (= H79), G164 (≠ H86), R191 (vs. gap), H192 (≠ V111)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (≠ A105), T184 (≠ S106), G202 (= G121), K218 (≠ R137), I219 (= I138), G220 (= G139), L226 (≠ I145), A236 (≠ V155)

Sites not aligning to the query:

binding cysteine: 91

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
38% identity, 70% coverage: 47:161/164 of query aligns to 126:243/244 of 8i06A

query
sites
8i06A

K

R

R

A

I

L

A

A

V

I

L

F

Q

L

H

Q

R

N

F

Q

W

V

S

S

A

V

V

S

C

F

G

Q

A

V

D

D

I

I

P

H

L

P

D

A

S

A

N

K

I

I

G

G

G

R

G

G

L

I

L

M

I

L

P

D

H
|
H

P

A

N

T

G

G

I

I

V

V

I

V

H

G

P

E

Q

T

A

A

K

V

I

I

G

E

P

D

N

D

C

V

L

S

I

I

F
x
L

Q
|
Q

Q

S

V

V

T

T

I

L

G

G

A

G

S

T

G

G

A

K

S

T

E

S

-

G

-

D

-

R

V

H

P

P

V

K

I

I

E

R

G

E

H

G

V

V

D

M

I

I

G
|
G

A
|
A

G

G

A

A

R

K

I

I

L

L

G

G

N

N

I

I

T

E

I

V

G

G

A

R

H

G

A

A

R

K

I

I

G
|
G

A
|
A

N

G

A

S

V

V

V

V

I

L

T

Q

D

P

I

V

P

P

A

P

N

H

A

T

T
|
T

A

A

V
x
A

G

G

I

V

P

P

G

A

K

R

I

I

binding coenzyme a: H158 (= H79), L177 (≠ F98), Q178 (= Q99), G203 (= G121), A204 (= A122), G221 (= G139), A222 (= A140), T235 (= T153), A237 (≠ V155)
binding cysteine: H158 (= H79)

Sites not aligning to the query:

binding cysteine: 93

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
40% identity, 55% coverage: 64:153/164 of query aligns to 165:265/267 of 3q1xA

query
sites
3q1xA

D

D

I

I

P

H

L

P

D

G

S

A

N

S

I

I

G

K

G

G

G

H

L

F

L

F

I

I

P

D

H

H

P

G

N

V

G

G

I

V

V

V

I

I

H

G

P

E

Q

T

A

A

K

I

I

I

G

G

P

E

N

W

C

C

L

R

I

I

F

Y

Q

Q

Q

S

V

V

T

T

I

L

G

G

A

A

-

M

-
x
H

-

F

-

Q

-

E

-

E

-

G

-

G

-

V

-

I

-

K

S

R

G

G

A

T

S

K

E
x
R

V
x
H

P

P

V

T

I

V

E

G

G

D

H

Y

V

V

D

T

I

I

G

G

A

T

G

G

A

A

R

K

I

V

L

L

G

G

N

N

I

I

T

I

I

V

G

G

A

S

H

H

A

V

R

R

I

I

G

G

A

A

N

N

A

C

V

W

V

I

I

D

T

R

D

D

I

V

P

D

A

S

N

N

A

Q

T

T

binding serine: H208 (vs. gap), R222 (≠ E110), H223 (≠ V111)

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
40% identity, 55% coverage: 64:153/164 of query aligns to 167:267/270 of 3p47A

query
sites
3p47A

D

D

I

I

P

H

L

P

D

G

S

A

N

S

I

I

G

K

G

G

G

H

L

F

L

F

I

I

P

D

H

H

P

G

N

V

G

G

I

V

V

V

I

I

H

G

P

E

Q

T

A

A

K

I

I

I

G

G

P

E

N

W

C

C

L

R

I

I

F

Y

Q

Q

Q

S

V

V

T

T

I

L

G

G

A
|
A

-

M

-
x
H

-

F

-

Q

-

E

-

E

-

G

-

G

-

V

-

I

-

K

S

R

G

G

A

T

S

K

E
x
R

V
x
H

P

P

V

T

I

V

E

G

G

D

H

Y

V

V

D

T

I

I

G

G

A

T

G

G

A

A

R

K

I

V

L

L

G

G

N

N

I

I

T

I

I

V

G

G

A

S

H

H

A

V

R

R

I

I

G

G

A

A

N

N

A

C

V

W

V

I

I

D

T

R

D

D

I

V

P

D

A

S

N

N

A

Q

T

T

binding cysteine: A208 (= A105), H210 (vs. gap), R224 (≠ E110), H225 (≠ V111)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
43% identity, 57% coverage: 59:151/164 of query aligns to 160:255/280 of 7bw9A

query
sites
7bw9A

A

S

V

V

C

T

G

G

A

I

D

D

I

I

P

H

L

P

D

G

S

T

N

S

I

I

G

G

G

N

G

H

L

F

L

F

I

I

P
x
D

H
|
H

P

G

N

V

G

G

I

V

V

V

I

I

H

G

P

E

Q

T

A

A

K

V

I

I

G

G

P

N

N

W

C

C

L

R

I

V

F

Y

Q

Q

Q

S

V

V

T

T

I

L

G

G

A
|
A

-

M

-

S

-

F

S

K

G

G

A

N

S

K

E
x
R

V
x
H

P

P

V

T

I

I

E

G

G

D

H

F

V

V

D

V

I

I

G

G

A

A

G

G

A

A

R

K

I

V

L

L

G

G

N

N

I

I

T

T

I

I

G

G

A

S

H

N

A

V

R

K

I

I

G

G

A

A

N

N

A

C

V

W

V

I

I

T

T

Q

D

N

I

I

P

D

A

Q

N

D

binding cysteine: D179 (≠ P78), H180 (= H79), A206 (= A105), R214 (≠ E110), H215 (≠ V111)

Sites not aligning to the query:

binding cysteine: 114, 115

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
36% identity, 49% coverage: 81:161/164 of query aligns to 63:139/290 of 4mzuB

query
sites
4mzuB

N

D

G

G

I

I

V

H

I

I

H

Q

P

D

Q

D

A

V

K
x
F

I

I

G

G

P

P

N
|
N

C

V

L

T

I

F

F

-

Q

-

Q

-

V

-

T
|
T

I
x
N

G
x
D

A
x
K

S

Q

G
x
P

A

R

S

S

E

L

V

K

P

T

V

I

I

V

E

K

G

K

H

G

V

A

D

S

I

I

G
|
G

A
|
A

G

N

A

S

R

T

I

I

L

L

G

P

N

G

I

I

T

L

I

I

G

G

A

E

H

N

A

A

R

M

I

V

G
|
G

A
|
A

N

G

A

A

V

V

V

I

I
x
T

T
x
K

D

N

I

V

P

P

A

D

N

N

A

A

T

I

A

V

V
x
I

G

G

I

N

P

P

G

G

K
x
R

I

I

binding coenzyme a: F72 (≠ K90), T80 (= T102), N81 (≠ I103), D82 (≠ G104), K83 (≠ A105), P85 (≠ G107), G99 (= G121), A100 (= A122), G117 (= G139), A118 (= A140), T123 (≠ I145), K124 (≠ T146), I133 (≠ V155), R138 (≠ K160)
binding magnesium ion: N76 (= N94)

Sites not aligning to the query:

binding coenzyme a: 199
binding magnesium ion: 58
binding thymine: 20, 21, 38, 39
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

4eaaA X-ray crystal structure of the h141n mutant of perosamine n- acetyltransferase from caulobacter crescentus in complex with coa and gdp-perosamine (see paper)
44% identity, 26% coverage: 120:162/164 of query aligns to 166:208/210 of 4eaaA

query
sites
4eaaA

I

L

G

G

A
x
V

G

G

A

A

R

R

I

V

L

I

G

P

N

G

I

V

T

T

I

I

G

G

A

A

H

D

A

T

R

I

I

V

G

G

A
|
A

N

G

A

G

V

V

V

V

I

V

T

R

D

D

I

L

P

P

A

D

N

S

A

V

T

L

A

A

V
x
I

G

G

I

V

P

P

G

A

K

K

I

I

R
x
K

binding coenzyme a: V168 (≠ A122), A186 (= A140), I201 (≠ V155), K208 (≠ R162)

Sites not aligning to the query:

binding coenzyme a: 150, 165
binding GDP-perosamine: 14, 15, 16, 35, 36, 37, 52, 70, 71

4ea9A X-ray structure of gdp-perosamine n-acetyltransferase in complex with transition state analog at 0.9 angstrom resolution (see paper)
44% identity, 26% coverage: 120:162/164 of query aligns to 164:206/207 of 4ea9A

query
sites
4ea9A

I

L

G
|
G

A
x
V

G

G

A

A

R

R

I

V

L

I

G

P

N

G

I

V

T

T

I

I

G

G

A

A

H

D

A

T

R

I

I

V

G

G

A
|
A

N

G

A

G

V

V

V

V

I

V

T

R

D

D

I

L

P

P

A

D

N

S

A

V

T

L

A

A

V
x
I

G

G

I

V

P

P

G

A

K

K

I

I

R
x
K

binding GDP-N-acetylperosamine-coenzyme A: G165 (= G121), V166 (≠ A122), A184 (= A140), I199 (≠ V155), K206 (≠ R162)

Sites not aligning to the query:

binding GDP-N-acetylperosamine-coenzyme A: 14, 15, 16, 35, 36, 37, 50, 68, 69, 145, 148, 163

4ea8A X-ray crystal structure of perb from caulobacter crescentus in complex with coenzyme a and gdp-n-acetylperosamine at 1 angstrom resolution (see paper)
44% identity, 26% coverage: 120:162/164 of query aligns to 164:206/207 of 4ea8A

query
sites
4ea8A

I

L

G
|
G

A
x
V

G

G

A

A

R

R

I

V

L

I

G

P

N

G

I

V

T

T

I

I

G

G

A

A

H

D

A

T

R

I

I

V

G

G

A
|
A

N

G

A

G

V

V

V

V

I

V

T

R

D

D

I

L

P

P

A

D

N

S

A

V

T

L

A

A

V

I

G

G

I

V

P

P

G

A

K

K

I

I

R
x
K

binding coenzyme a: G165 (= G121), V166 (≠ A122), A184 (= A140), K206 (≠ R162)

Sites not aligning to the query:

binding coenzyme a: 145, 163
binding GDP-N-acetylperosamine: 14, 15, 16, 35, 36, 37, 50, 68, 69

Query Sequence

>WP_012466757.1 NCBI__GCF_000020465.1:WP_012466757.1
MISATEPDWSREKPVPGRFEPGKKMLAALRRYQKNSRKADPVSQTIKRIAVLQHRFWSAV
CGADIPLDSNIGGGLLIPHPNGIVIHPQAKIGPNCLIFQQVTIGASGASEVPVIEGHVDI
GAGARILGNITIGAHARIGANAVVITDIPANATAVGIPGKIRQT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory