SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012466868.1 NCBI__GCF_000020465.1:WP_012466868.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q42601 Carbamoyl phosphate synthase arginine-specific large chain, chloroplastic; CPS; CPSase; Ammonium-dependent carbamoyl phosphate synthetase; Arginine-specific carbamoyl phosphate synthetase, ammonia chain; Glutamine-dependent carbamoyl phosphate synthetase; Protein VENOSA 3; EC 6.3.4.16; EC 6.3.5.5 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
48% identity, 98% coverage: 12:617/618 of query aligns to 568:1181/1187 of Q42601

query
sites
Q42601

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G587 (= G31) mutation to E: In ven3-3; reticulate leaf phenotype.
A844 (= A275) mutation to T: In ven3-4; reduced plant size and reticulate leaf phenotype.
P1014 (= P449) mutation to L: In ven3-1; reticulate leaf phenotype.

Sites not aligning to the query:

149 P→L: In ven3-2; reduced plant size and reticulate leaf phenotype.

1bxrA Structure of carbamoyl phosphate synthetase complexed with the atp analog amppnp (see paper)
49% identity, 96% coverage: 24:614/618 of query aligns to 489:1070/1073 of 1bxrA

query
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1bxrA

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active site: G507 (≠ K42), K634 (≠ Q168), R715 (= R249), G721 (= G255), G722 (= G256), S745 (= S279), E761 (= E295), D769 (= D303), Q829 (= Q363), E841 (= E375), N843 (= N377), R848 (= R382), P901 (≠ V441)
binding phosphoaminophosphonic acid-adenylate ester: R675 (= R209), V713 (≠ L247), R715 (= R249), L720 (= L254), G721 (= G255), G722 (= G256), M725 (= M259), D753 (= D287), F755 (= F289), L756 (= L290), E761 (= E295), A785 (= A319), G786 (= G320), V787 (≠ I321), H788 (= H322), Q829 (= Q363), E841 (= E375), N843 (= N377), R848 (= R382)
binding manganese (ii) ion: Q829 (= Q363), E841 (= E375), E841 (= E375), N843 (= N377)
binding L-ornithine: E783 (= E317), D791 (= D325), E892 (= E432), L907 (= L447), D1041 (≠ V583), T1042 (= T584)

Sites not aligning to the query:

active site: 129, 169, 174, 176, 202, 215, 243, 283, 285, 299, 301, 303, 307, 338
binding phosphoaminophosphonic acid-adenylate ester: 129, 167, 169, 174, 175, 176, 210, 211, 215, 240, 241, 243, 244, 285, 299, 306, 376
binding manganese (ii) ion: 299, 301

P00968 Carbamoyl phosphate synthase large chain; Carbamoyl phosphate synthetase ammonia chain; EC 6.3.4.16; EC 6.3.5.5 from Escherichia coli (strain K12) (see 6 papers)
49% identity, 96% coverage: 24:614/618 of query aligns to 489:1070/1073 of P00968

query
sites
P00968

L

L

I

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K

Q

A

L

K

K

E

R

H

K

G

G

F

F

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A

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C

A

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R

L

L

A

A

N

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I

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F

A

K

G

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K

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L

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Q

Y

Y

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H

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P

V

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F

Y

K

K

T

R

V

V

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D

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C

C

A

A

E

E

F

F

D

A

A

T

K

D

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T

P

A

Y

Y

H

M

Y

Y

S

S

T

T

Y

Y

D

E

E

E

N

C

E

E

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A

V

N

R

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S

-

T

D

D

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R

K

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M

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L

L

G

G

P

P

N

N

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Q

G

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F

D

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A

A

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R

E

E

A

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Y

Y

E

E

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M

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V

N

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E

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-

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A

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A

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D

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C

A

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A

R715 (= R249) binding ATP
H754 (≠ R288) binding ATP
L756 (= L290) binding ATP
E761 (= E295) binding ATP
G786 (= G320) binding ATP
V787 (≠ I321) binding ATP
H788 (= H322) binding ATP
S789 (= S323) binding ATP
Q829 (= Q363) binding ATP; binding Mn(2+)
E841 (= E375) binding ATP; binding Mn(2+); binding Mn(2+)
N843 (= N377) binding Mn(2+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
129 binding ATP
169 binding ATP
175 binding ATP
176 binding ATP
208 binding ATP
210 binding ATP
215 binding ATP
241 binding ATP
242 binding ATP
243 binding ATP
285 binding ATP; binding Mn(2+)
299 binding ATP; binding Mn(2+); binding Mn(2+)
301 binding Mn(2+)

1t36A Crystal structure of e. Coli carbamoyl phosphate synthetase small subunit mutant c248d complexed with uridine 5'-monophosphate (see paper)
48% identity, 96% coverage: 24:614/618 of query aligns to 489:1055/1058 of 1t36A

query
sites
1t36A

L

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N

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K

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T

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M

A

A

I

L

D

N

C

A

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A

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T

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E

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F

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A

A

active site: G507 (≠ K42), K634 (≠ Q168), R715 (= R249), E746 (= E295), D754 (= D303), Q814 (= Q363), E826 (= E375), N828 (= N377), R833 (= R382), P886 (≠ V441)
binding adenosine-5'-diphosphate: R715 (= R249), M718 (= M259), F740 (= F289), L741 (= L290), E746 (= E295), A770 (= A319), G771 (= G320), V772 (≠ I321), H773 (= H322), E826 (= E375), P894 (= P449)
binding manganese (ii) ion: Q814 (= Q363), E826 (= E375)
binding L-ornithine: E768 (= E317), D776 (= D325), E877 (= E432), L892 (= L447), D1026 (≠ V583), T1027 (= T584)
binding uridine-5'-monophosphate: K939 (= K494), T959 (= T515), G961 (= G517), T962 (= T518), K978 (= K534), N1000 (= N557), T1001 (= T558), T1002 (≠ P559), S1011 (≠ E568), I1014 (= I571)

Sites not aligning to the query:

active site: 129, 169, 174, 176, 202, 215, 243, 283, 285, 299, 301, 303, 307, 338
binding adenosine-5'-diphosphate: 129, 167, 169, 174, 175, 176, 208, 210, 211, 215, 240, 241, 242, 243, 285, 298, 299, 376
binding manganese (ii) ion: 285, 299, 299, 301
binding phosphate ion: 174, 175, 243, 299, 301, 303, 306

1c3oA Crystal structure of the carbamoyl phosphate synthetase: small subunit mutant c269s with bound glutamine (see paper)
48% identity, 96% coverage: 24:614/618 of query aligns to 489:1055/1058 of 1c3oA

query
sites
1c3oA

L

L

I

R

K

Q

A

L

K

K

E

R

H

K

G

G

F

F

S

A

D

D

C

A

Q

R

L

L

A

A

N

K

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L

F

A

K
x
G

T

V

T

R

E

E

T

A

V

E

I

I

R

R

T

K

L

L

R

R

K

D

Q

Q

Y

Y

G

D

V

L

E

H

S

P

V

V

F

Y

K

K

T
x
R

V

V

D

D

T

T

C

C

A

A

E

E

F

F

D
x
A

A
x
T

K

D

T

T

P

A

Y

Y

H

M

Y

Y

S

S

T

T

Y

Y

D

E

E

E

N

C

E

E

S

A

V
x
N

R

P

S

S

-

T

D

D

R

R

K

E

K

K

V

I

I

M

I

V

L

L

G

G

P

P

N

N

R

R

I

I

G

G

Q

Q

G

G

I

I

E

E

F

F

D

D

Y

Y

C

C

V

V

Q

H

A

A

V

S

F

L

A

A

L

L

R

R

E

E

A

D

G

G

Y

Y

E

E

T

T

I

I

M

M

V

V

N

N

C

C

N

N

P

P

E

E

T

T

V

V

S

S

T

T

D

D

Y

Y

D

D

I

T

A

S

D

D

K

R

L

L

Y

Y

F

F

E

E

P

P

L

V

T

T

F

L

E

E

D

D

T

V

I

L

R

E

I

I

I

V

E

R

H

I

E

E

Q
x
K

P

P

L

K

G

G

V

V

I

I

V

V

S

Q

F

Y

G

G

Q

Q

T

T

P

P

L

L

K

K

L

L

S

A

T

R

K

A

L

L

D

E

E

A

A

A

G

G

V

V

T

P

I

V

L

I

G

G

T

T

S

S

S

P

K

D

G

A

I

I

D

D

L

R

A

A

E

E

D

D

R

R

K

E

K

R

F

F

G

Q

A

H

L

A

L

V

E

E

K

R

L

L

D

K

I

L

L

K

H

Q

P

P

D

A

Y

N

G

A

T

T

A

V

I

T

C

A

F

I

D

E

E

M

A

A

L

V

A

E

I

K

T

A

E

K

R

E

I

I

G

G

Y

Y

P

P

V

L

L

V

V

V

R
|
R

P

P

S

-

Y

-

V

-

L

-

G

-

R

-

A

A

M
|
M

K

E

I

I

I

V

Y

Y

N

D

K

E

D

A

S

D

L

L

K

R

E

R

Y

Y

V

F

D

Q

Q

T

A

A

L

-

F

-

I

-

S

-

E

-

K

-

Y

-

P

-

L

V

L

L

I

L

D

D

R

H

F
|
F

L
|
L

E

D

T

D

A

A

V

V

E
|
E

F

V

D

D

I

V

D

D

A

A

I

I

A

C

D
|
D

T

G

T

E

D

M

C

V

V

L

I

I

S

G

G

G

I

I

M

M

Q

E

H

H

V

I

E
|
E

A

Q

A
|
A

G
|
G

I
x
V

H
|
H

S
|
S

G

G

D
|
D

S

S

T

A

S

C

I

S

L

L

P

P

Y

A

R

Y

N

T

I

L

S

S

Q

Q

E

E

V

I

I

Q

N

D

T

V

M

M

K

R

A

Q

Y

Q

T

V

R

Q

T

K

L

L

A

A

E

F

H

E

L

L

K

Q

V

V

V

R

G

G

L

L

M

M

N

N

V

V

Q
|
Q

Y

F

A

A

V

V

Q

K

N

N

E

N

S

E

V

V

Y

Y

V

L

I

I

E
|
E

V

V

N
|
N

P

P

R

R

A

A

S

A

R
|
R

T

T

V

V

P

P

F

F

V

V

G

S

K

K

A

A

T

T

A

G

V

V

P

P

V

L

V

A

K

K

I

V

A

A

T

A

R

R

V

V

M

M

L

A

G

G

E

K

K

S

L

L

S

A

D

-

L

-

R

-

K

-

E

-

Y

-

D

E

L

Q

K

G

D

V

C

T

D

K

E

E

L

V

G

I

M

P

K

P

H

Y

M

Y

A

S

I

V

K

K

E
|
E

P

V

V

V

F

L

P

P

F

F

S

N

K

K

F

F

V
x
P

K

G

S

V

G

D

V

P

Y

L

L
|
L

G

G

P

P

E

E

M

M

R

R

S

S

T

T

G

G

E

E

A

V

M

M

S

G

L

V

A

G

E

R

Q

T

F

F

P

A

E

E

A

A

F

F

A

A

K

K

A

A

Y

Q

Q

L

A

G

A

S

N

N

M

S

E

T

L

M

P

K

L

K

S

H

G

G

S

R

V

A

F

L

I

L

S

S

V

V

N

R

D

E

Q

G

D

D

K

K

S

-

Q

E

R

R

I

V

A

D

I

L

A

A

K

A

E

K

L

L

Y

L

R

K

M

Q

D

G

F

F

D

E

L

L

V

D

A

A

T

T

A

H

G

G

T

T

H

A

R

I

F

V

L

L

I

G

E

E

N

A

G

G

I

I

E

N

C

P

K

R

K

L

V

V

F

N

K

K

V

V

G

-

E

H

E

E

G

G

R

R

P

P

N

H

I

I

F

Q

D

D

I

R

I

I

K

K

H

N

G

G

K

E

I

Y

D

T

F

Y

V

I

I

I

N

N

T

T

P

T

R

S

G

G

E

R

K

R

A

A

L

I

H

E

D

D

E

S

E

R

A

V

I

I

G

R

A

R

A

S

S

A

V

L

L

Q

S

Y

N

K

V

V

P

H

F

Y

V
x
D

T
|
T

T

T

I

L

E

N

A

G

E

F

A

A

S

T

V

A

Q

M

A

A

I

L

D

N

C

A

I

D

R

A

R

T

Q

E

E

K

F

-

G

-

V

V

K

I

S

S

L

V

Q

Q

E

E

Y

M

S

H

A

A

active site: G507 (≠ K42), K634 (≠ Q168), R715 (= R249), E746 (= E295), D754 (= D303), Q814 (= Q363), E826 (= E375), N828 (= N377), R833 (= R382), P886 (≠ V441)
binding adenosine-5'-diphosphate: R715 (= R249), M718 (= M259), F740 (= F289), L741 (= L290), E746 (= E295), A770 (= A319), G771 (= G320), V772 (≠ I321), H773 (= H322), S774 (= S323), E826 (= E375)
binding glutamine: R528 (≠ T63), A537 (≠ D72), T538 (≠ A73), N554 (≠ V89)
binding manganese (ii) ion: Q814 (= Q363), E826 (= E375)
binding L-ornithine: E768 (= E317), D776 (= D325), E877 (= E432), L892 (= L447), D1026 (≠ V583), T1027 (= T584)

Sites not aligning to the query:

active site: 129, 169, 174, 176, 202, 215, 243, 283, 285, 299, 301, 303, 307, 338
binding adenosine-5'-diphosphate: 129, 167, 169, 174, 176, 210, 211, 215, 240, 241, 243, 244, 285, 298, 299, 376
binding manganese (ii) ion: 285, 299, 299, 301
binding phosphate ion: 174, 175, 243, 299, 301, 303, 306

1ce8A Carbamoyl phosphate synthetase from escherichis coli with complexed with the allosteric ligand imp (see paper)
48% identity, 96% coverage: 24:614/618 of query aligns to 489:1055/1058 of 1ce8A

query
sites
1ce8A

L

L

I

R

K

Q

A

L

K

K

E

R

H

K

G

G

F

F

S

A

D

D

C

A

Q

R

L

L

A

A

N

K

I

L

F

A

K
x
G

T

V

T

R

E

E

T

A

V

E

I

I

R

R

T

K

L

L

R

R

K

D

Q

Q

Y

Y

G

D

V

L

E

H

S

P

V

V

F

Y

K

K

T
x
R

V

V

D

D

T

T

C

C

A

A

E

E

F

F

D
x
A

A
x
T

K

D

T

T

P

A

Y

Y

H

M

Y

Y

S

S

T

T

Y

Y

D

E

E

E

N

C

E
|
E

S

A

V
x
N

R

P

S

S

-

T

D

D

R

R

K

E

K

K

V

I

I

M

I

V

L

L

G

G

P

P

N

N

R

R

I

I

G

G

Q

Q

G

G

I

I

E

E

F

F

D

D

Y

Y

C

C

V

V

Q

H

A

A

V

S

F

L

A

A

L

L

R

R

E

E

A

D

G

G

Y

Y

E

E

T

T

I

I

M

M

V

V

N

N

C

C

N

N

P

P

E

E

T

T

V

V

S

S

T

T

D

D

Y

Y

D

D

I

T

A

S

D

D

K

R

L

L

Y

Y

F

F

E

E

P

P

L

V

T

T

F

L

E

E

D

D

T

V

I

L

R

E

I

I

I

V

E

R

H

I

E

E

Q
x
K

P

P

L

K

G

G

V

V

I

I

V

V

S

Q

F

Y

G

G

Q

Q

T

T

P

P

L

L

K

K

L

L

S

A

T

R

K

A

L

L

D

E

E

A

A

A

G

G

V

V

T

P

I

V

L

I

G

G

T

T

S

S

S

P

K

D

G

A

I

I

D

D

L

R

A

A

E

E

D

D

R

R

K

E

K

R

F

F

G

Q

A

H

L

A

L

V

E

E

K

R

L

L

D

K

I

L

L

K

H

Q

P

P

D

A

Y

N

G

A

T

T

A

V

I

T

C

A

F

I

D

E

E

M

A

A

L

V

A

E

I

K

T

A

E

K

R

E

I

I

G

G

Y

Y

P

P

V

L

L

V

V

V

R
|
R

P

P

S

-

Y

-

V

-

L

-

G

-

R

-

A

A

M

M

K

E

I

I

I

V

Y

Y

N

D

K

E

D

A

S

D

L

L

K

R

E

R

Y

Y

V

F

D

Q

Q

T

A

A

L

-

F

-

I

-

S

-

E

-

K

-

Y

-

P

-

L

V

L

L

I

L

D

D

R

H

F
|
F

L
|
L

E

D

T

D

A

A

V

V

E
|
E

F

V

D

D

I

V

D

D

A

A

I

I

A

C

D
|
D

T

G

T

E

D

M

C

V

V

L

I

I

S

G

G

G

I

I

M

M

Q

E

H

H

V

I

E
|
E

A

Q

A
|
A

G
|
G

I
x
V

H
|
H

S
|
S

G

G

D
|
D

S

S

T

A

S

C

I

S

L

L

P

P

Y

A

R

Y

N

T

I

L

S

S

Q

Q

E

E

V

I

I

Q

N

D

T

V

M

M

K

R

A

Q

Y

Q

T

V

R

Q

T

K

L

L

A

A

E

F

H

E

L

L

K

Q

V

V

V

R

G

G

L

L

M

M

N

N

V

V

Q
|
Q

Y

F

A

A

V

V

Q

K

N

N

E

N

S

E

V

V

Y

Y

V

L

I

I

E
|
E

V

V

N
|
N

P

P

R

R

A

A

S

A

R
|
R

T

T

V

V

P

P

F

F

V

V

G

S

K

K

A

A

T

T

A

G

V

V

P

P

V

L

V

A

K

K

I

V

A

A

T

A

R

R

V

V

M

M

L

A

G

G

E

K

K

S

L

L

S

A

D

-

L

-

R

-

K

-

E

-

Y

-

D

E

L

Q

K

G

D

V

C

T

D

K

E

E

L

V

G

I

M

P

K

P

H

Y

M

Y

A

S

I

V

K

K

E
|
E

P

V

V

V

F

L

P

P

F

F

S

N

K

K

F

F

V
x
P

K

G

S

V

G

D

V

P

Y

L

L
|
L

G

G

P

P

E

E

M

M

R

R

S

S

T

T

G

G

E

E

A

V

M

M

S

G

L

V

A

G

E

R

Q

T

F

F

P

A

E

E

A

A

F

F

A

A

K

K

A

A

Y

Q

Q

L

A

G

A

S

N

N

M

S

E

T

L

M

P

K

L

K

S

H

G

G

S

R

V

A

F

L

I

L

S
|
S

V

V

N

R

D

E

Q

G

D

D

K
|
K

S

-

Q

E

R

R

I

V

A

D

I

L

A

A

K

A

E

K

L

L

Y

L

R

K

M

Q

D

G

F

F

D

E

L

L

V

D

A

A

T
|
T

A

H

G
|
G

T
|
T

H

A

R

I

F

V

L

L

I

G

E

E

N

A

G

G

I

I

E

N

C

P

K

R

K

L

V

V

F

N

K
|
K

V
|
V

G

-

E

H

E

E

G

G

R

R

P

P

N

H

I
|
I

F

Q

D

D

I

R

I

I

K

K

H

N

G

G

K

E

I

Y

D

T

F

Y

V

I

I

I

N
|
N

T
|
T

P
x
T

R

S

G

G

E

R

K

R

A

A

L

I

H

E

D
|
D

E
x
S

E

R

A
x
V

I

I

G

R

A

R

A

S

S

A

V

L

L

Q

S

Y

N

K

V

V

P

H

F
x
Y

V
x
D

T
|
T

T

T

I

L

E

N

A

G

E

F

A

A

S

T

V

A

Q

M

A

A

I

L

D

N

C

A

I

D

R

A

R

T

Q

E

E

K

F

-

G

-

V

V

K

I

S

S

L

V

Q

Q

E

E

Y

M

S

H

A

A

active site: G507 (≠ K42), K634 (≠ Q168), R715 (= R249), E746 (= E295), D754 (= D303), Q814 (= Q363), E826 (= E375), N828 (= N377), R833 (= R382), P886 (≠ V441)
binding adenosine-5'-diphosphate: R715 (= R249), F740 (= F289), L741 (= L290), E746 (= E295), A770 (= A319), G771 (= G320), V772 (≠ I321), H773 (= H322), S774 (= S323), E826 (= E375)
binding inosinic acid: S933 (= S488), K939 (= K494), T959 (= T515), G961 (= G517), T962 (= T518), K978 (= K534), V979 (= V535), I986 (= I543), N1000 (= N557), T1001 (= T558), T1002 (≠ P559), D1010 (= D567), S1011 (≠ E568), V1013 (≠ A570)
binding manganese (ii) ion: Q814 (= Q363), E826 (= E375)
binding L-ornithine: R528 (≠ T63), A537 (≠ D72), T538 (≠ A73), E552 (= E87), N554 (≠ V89), E768 (= E317), D776 (= D325), E877 (= E432), L892 (= L447), Y1025 (≠ F582), D1026 (≠ V583), T1027 (= T584)

Sites not aligning to the query:

active site: 129, 169, 174, 176, 202, 215, 243, 283, 285, 299, 301, 303, 307, 338
binding adenosine-5'-diphosphate: 129, 167, 169, 174, 176, 210, 211, 215, 240, 241, 242, 243, 285, 298, 299, 376
binding manganese (ii) ion: 174, 285, 299, 299, 301
binding phosphate ion: 174, 175, 243, 299, 301, 303, 306

1a9xA Carbamoyl phosphate synthetase: caught in the act of glutamine hydrolysis (see paper)
47% identity, 96% coverage: 24:614/618 of query aligns to 489:1055/1058 of 1a9xA

query
sites
1a9xA

L

L

I

R

K

Q

A

L

K

K

E

R

H

K

G

G

F

F

S

A

D

D

C

A

Q

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L

L

A

A

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K

I

L

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A

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x
G

T

V

T

R

E

E

T

A

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I

I

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L

L

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Q

Y

Y

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Y

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K

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I

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N

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I

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G

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Q

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G

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I

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V

V

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A

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G

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Y

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M

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V

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N

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K

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|
R

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D

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|
F

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|
E

F

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D

D

I

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D

T

G

T

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I

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M

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E

H

H

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I

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E

A

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A

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G

I
x
V

H
|
H

S

S

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D
|
D

S

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C

I

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L

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P

Y

A

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Y

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G

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L

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M

N

N

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Q

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K

N

N

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V

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G

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L

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D

-

L

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|
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P

P

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G

G

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K

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A

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G

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T

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A

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I

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L

L

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G

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E

N

A

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G

I

I

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N

C

P

K

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L

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N

K

K

V

V

G

-

E

H

E

E

G

G

R

R

P

P

N

H

I

I

F

Q

D

D

I

R

I

I

K

K

H

N

G

G

K

E

I

Y

D

T

F

Y

V

I

I

I

N

N

T

T

P

T

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S

G

G

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K

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A

A

L

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E

D

D

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A

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I

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A

R

A

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S

A

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L

L

Q

S

Y

N

K

V

V

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H

F
x
Y

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x
D

T
|
T

T

T

I

L

E

N

A

G

E

F

A

A

S

T

V

A

Q

M

A

A

I

L

D

N

C

A

I

D

R

A

R

T

Q

E

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F

-

G

-

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A

A

active site: G507 (≠ K42), K634 (≠ Q168), D754 (= D303), P886 (≠ V441)
binding adenosine-5'-diphosphate: R715 (= R249), M718 (= M259), F740 (= F289), L741 (= L290), E746 (= E295), A770 (= A319), G771 (= G320), V772 (≠ I321), H773 (= H322), E826 (= E375)
binding manganese (ii) ion: Q814 (= Q363), E826 (= E375)
binding L-ornithine: E768 (= E317), D776 (= D325), E877 (= E432), L892 (= L447), Y1025 (≠ F582), D1026 (≠ V583), T1027 (= T584)

Sites not aligning to the query:

active site: 202, 338
binding adenosine-5'-diphosphate: 129, 167, 169, 174, 175, 176, 210, 215, 240, 241, 242, 243, 244, 285, 298, 299, 376
binding manganese (ii) ion: 285, 299, 299, 301
binding phosphate ion: 175, 243, 299, 301, 303, 306

P07259 Multifunctional protein URA2; Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase; CPSase-ATCase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
37% identity, 94% coverage: 14:596/618 of query aligns to 900:1485/2214 of P07259

query
sites
P07259

G

G

L

T

A

K

S

E

K

E

L

L

P

P

K

S

K

L

T

V

L

L

I

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A

A

K

K

E

Q

H

L

G

G

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F

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D

D

D

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Q

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A

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V

A

I

I

R

R

T

R

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L

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R

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K

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E

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Y

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G

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T

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D

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T

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C

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A

A

E

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F

D

P

A

A

K

Y

T

T

P

N

Y

Y

H

L

Y

Y

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M

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T

Y

Y

D

N

E

A

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N

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E

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S

F

D

D

R

D

K

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K

G

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M

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L

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Y

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E

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F

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Y

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C

A

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Q

T

A

A

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V

F

R

A

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L

R

R

E

A

A

N

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N

Y

I

E

K

T

T

I

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M

V

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N

N

C

Y

N

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P

P

E

E

T

T

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D

D

Y

Y

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E

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A

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D

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L

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Y

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F

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Q

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A

A

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N

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Y

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A

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A

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M

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N

N

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I

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A

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K

N

D

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N

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E

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I

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S

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K

A

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A

G

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N

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I

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A

A

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A

A

I

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G

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E

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A

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A

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A

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Y

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L

A

A

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N

G

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F

E

K

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L

P

P

L

-

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K

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N

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I

F

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I

L

S

S

V

I

N

G

D

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Q

Y

D

K

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E

I

L

A

S

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S

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Q

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K

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L

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Y

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N

M

M

D

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F

Y

D

K

L

L

V

F

A

A

T

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A

S

G

G

T

T

H

A

R

D

F

F

L

L

I

S

E

E

N

H

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G

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I

E

A

C

V

K

Q

-

Y

-

L

K

E

V

V

F

L

K

N

V

K

G

D

E

D

G

D

R

Q

P

K

N

S

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E

F

Y

D

S

I

L

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T

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Q

H

H

-

L

-

A

-

N

G

N

K

E

I

I

D

D

F

L

V

Y

I

I

N

N

T

L

P

P

R

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G

A

E

N

K

R

A

F

L

R

H

R

D

P

E

A

E

S

A

Y

I

V

G

S

A

K

A

G

-

Y

-

K

-

T

-

R

-

L

S

A

V

V

L

D

S

Y

N

S

V

V

P

P

F

L

V

V

T

T

T

N

I

V

E

K

A

C

A

A

E

K

A

L

S

L

V

I

Q

E

A

A

I

I

Sites not aligning to the query:

1857 modified: Phosphoserine; by PKA

P31327 Carbamoyl-phosphate synthase [ammonia], mitochondrial; Carbamoyl-phosphate synthetase I; CPSase I; EC 6.3.4.16 from Homo sapiens (Human) (see 22 papers)
37% identity, 99% coverage: 7:615/618 of query aligns to 886:1494/1500 of P31327

query
sites
P31327

S

N

L

M

S

E

Q
x
K

E

T

L

L

S
x
K

G

G

L

L

A

N

S

S

K

E

-

S

L

M

P

T

K

E

T

T

L

L

I
x
K

K

R

A

A

K
|
K

E

E

H

I

G
|
G

F

F

S
|
S

D
|
D

C
x
K

Q

Q

L

I

A
x
S

N
x
K

I

C

F

L

K

G

T

L

T

T

E

E

T

A

V

Q

I

T

R

R

T

E

L

L

R
|
R

K

L

Q

K

Y

K

G

N

V
x
I

E

H

S

P

V

W

F

V

K

K

T

Q

V

I

D

D

T

T

C

L

A

A

A
|
A

E

E

F

Y

D

P

A

S

K

V

T

T

P

N

Y

Y

H
x
L

Y
|
Y

S

V

T

T

Y
|
Y

D

N

E
x
G

E

Q

N

E

E

H

S

D

V

V

R

N

S

F

D

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K889 (≠ Q10) modified: N6-glutaryllysine; alternate
K892 (≠ S13) modified: N6-glutaryllysine; alternate
K905 (≠ I25) modified: N6-glutaryllysine
K908 (= K28) modified: N6-glutaryllysine; alternate
G911 (= G31) to E: in CPS1D; significant decrease in protein yield and enzyme activity; dbSNP:rs1388955593; to V: in CPS1D; significant decrease in protein yield and enzyme activity
S913 (= S33) to L: in CPS1D; significant decrease in protein yield and partial loss of enzyme activity; dbSNP:rs754706559
D914 (= D34) to G: in CPS1D; significant decrease in protein yield and enzyme activity; to H: in CPS1D; significant decrease in protein yield and enzyme activity; dbSNP:rs765484849
K915 (≠ C35) modified: N6-glutaryllysine; alternate
S918 (≠ A38) to P: in CPS1D; significant decrease in protein yield and enzyme activity
K919 (≠ N39) modified: N6-glutaryllysine; alternate
R932 (= R52) to T: in CPS1D; significant decrease in protein yield and partial loss of enzyme activity
I937 (≠ V57) to N: in CPS1D; associated with R-401; significant decrease in protein yield and enzyme activity; dbSNP:rs760714614
A949 (= A69) to T: in CPS1D; partial loss of enzyme activity and significant decrease in thermal stability; dbSNP:rs537170841
L958 (≠ H78) to P: in CPS1D; significant decrease in protein yield and enzyme activity
Y959 (= Y79) to C: in CPS1D; significant decrease in protein yield and thermal stability; partial loss of enzyme activity; dbSNP:rs1191587211
Y962 (= Y82) to C: in CPS1D; significant decrease in protein yield and partial loss of enzyme activity; dbSNP:rs955666400
G964 (≠ E84) to D: in CPS1D; significant decrease in protein yield and enzyme activity; dbSNP:rs534815243
I986 (= I106) to T: in CPS1D; associated with V-304; dbSNP:rs1553516442
G987 (= G107) to C: in CPS1D; may affect splicing; dbSNP:rs1553516443
K1074 (≠ T194) modified: N6-glutaryllysine; alternate
K1150 (≠ E270) modified: N6-glutaryllysine
K1168 (≠ R288) modified: N6-glutaryllysine; alternate
K1183 (≠ D303) modified: N6-glutaryllysine; alternate
I1215 (= I335) to V: in CPS1D; uncertain significance; dbSNP:rs141373204
K1224 (= K344) modified: N6-glutaryllysine
I1254 (= I374) to F: in CPS1D; uncertain significance
F1266 (= F386) to S: in dbSNP:rs1047886
M1283 (= M403) to L: in dbSNP:rs1047887
K1356 (≠ E478) modified: N6-glutaryllysine; alternate
K1360 (≠ S482) modified: N6-glutaryllysine; alternate
LIGI 1363:1366 (≠ FISV 486:489) natural variant: Missing (in CPS1D; uncertain significance)
G1376 (≠ A500) to S: no functional consequences; no negative effect on protein stability, enzyme activity and thermal stability; dbSNP:rs140578009
A1378 (= A502) to T: in CPS1D; significant reduction in thermal stability; dbSNP:rs1245373037
L1381 (= L505) to S: in CPS1D; significant loss of protein stability
T1406 (≠ K530) to N: probable risk factor for PHN; dbSNP:rs1047891
P1411 (≠ V535) to L: in CPS1D; modestly decreases enzyme activity; dbSNP:rs1202306773
T1443 (vs. gap) to A: in CPS1D; almost complete loss of enzyme activity; approximately 10-fold decrease in the apparent Vmax for bicarbonate, ammonia and ATP; decreased affinity for NAG
R1453 (≠ G572) to Q: in CPS1D; the enzyme is inactive; to W: in CPS1D; the enzyme is inactive; dbSNP:rs933813349
K1479 (≠ C598) modified: N6-glutaryllysine; alternate
K1486 (= K607) modified: N6-glutaryllysine; alternate
Y1491 (= Y612) to H: in CPS1D; triggers a large decrease in the apparent affinity for N-acetyl-L-glutamate (NAG); dbSNP:rs1553519513

Sites not aligning to the query:

55 modified: N6-glutaryllysine; alternate
123 S → F: in CPS1D; modestly decreases enzyme activity; S → Y: in CPS1D; uncertain significance
171 modified: N6-glutaryllysine; alternate
174 R → W: in CPS1D; uncertain significance; dbSNP:rs1553509661
176 modified: N6-glutaryllysine
207 modified: N6-glutaryllysine; alternate
210 modified: N6-glutaryllysine; alternate
214 modified: N6-glutaryllysine; alternate
219 modified: N6-glutaryllysine; alternate
228 modified: N6-glutaryllysine; alternate
237 modified: N6-glutaryllysine
280 modified: N6-glutaryllysine; alternate
304 A → V: in CPS1D; associated with T-986; dbSNP:rs775920437
307 modified: N6-glutaryllysine; alternate
310 modified: N6-glutaryllysine; alternate
337 H → R: in CPS1D; modestly decreases enzyme activity; dbSNP:rs28940283
344 T → A: no negative effect on protein stability, enzyme activity and thermal stability; dbSNP:rs1047883
355 N → D: in CPS1D; around 80% decrease in enzyme activity; significant reduction in thermal stability; approximately 4-fold decrease in the apparent Vmax for ATP, bicarbonate and ammonia; dbSNP:rs1472190012
389 Y → C: in CPS1D; around 40% decrease in enzyme activity; significant loss of thermal stability
390 L → R: in CPS1D; significant loss of protein stability
401 G → R: in CPS1D; uncertain significance; associated with N-937 in a patient; dbSNP:rs760895692
402 modified: N6-glutaryllysine; alternate
412 modified: N6-glutaryllysine; alternate
438 A → P: in CPS1D; almost complete loss of enzyme activity; dbSNP:rs772497399
453 modified: N6-glutaryllysine; alternate
458 modified: N6-glutaryllysine; alternate
527 modified: N6-glutaryllysine; alternate
530 G → V: found in a patient with VACTERL syndrome and postsurgical PHN; uncertain significance; dbSNP:rs1250316045
532 modified: N6-glutaryllysine; alternate
544 T → M: in CPS1D; almost complete loss of enzyme activity; approximately 60-fold increase in the apparent Km for bicarbonate and approximately 4-fold respective decrease and increase in the apparent Vmax and Km for ammonia; dbSNP:rs121912592
553 modified: N6-glutaryllysine; alternate
678 Q → P: in CPS1D; results in a poor enzyme expression and solubility; hampers correct enzyme folding
728 modified: N6-glutaryllysine
757 modified: N6-glutaryllysine; alternate
772 modified: N6-glutaryllysine; alternate
774 P → L: in CPS1D; the enzyme is inactive
793 modified: N6-glutaryllysine; alternate
811 modified: N6-glutaryllysine; alternate
841 modified: N6-glutaryllysine; alternate
843 L → S: in CPS1D; associated in cis with E-875; causes 70% decrease of enzyme activity; significant decrease in protein yield
850 R → C: in CPS1D; moderate decrease in protein yield and partial loss of enzyme activity; dbSNP:rs1015051007; R → H: in CPS1D; partial loss of enzyme activity; dbSNP:rs767694281
856 modified: N6-glutaryllysine; alternate
869 modified: N6-glutaryllysine
871 T → P: in CPS1D; significant decrease in protein yield and enzyme activity
875 modified: N6-glutaryllysine; alternate; K → E: associated in cis with S-843 in a patient with carbamoyl-phosphate synthase deficiency; does not affect enzyme activity; significant decrease in protein yield and thermal stability; dbSNP:rs147062907

Q8C196 Carbamoyl-phosphate synthase [ammonia], mitochondrial; Carbamoyl-phosphate synthetase I; CPSase I; EC 6.3.4.16 from Mus musculus (Mouse) (see 2 papers)
36% identity, 99% coverage: 7:615/618 of query aligns to 886:1494/1500 of Q8C196

query
sites
Q8C196

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D

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G

N

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-

T

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K

A

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H

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A

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C

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I

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K1291 (≠ L411) modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate

Sites not aligning to the query:

44 modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate
287 modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate

P07756 Carbamoyl-phosphate synthase [ammonia], mitochondrial; Carbamoyl-phosphate synthetase I; CPSase I; EC 6.3.4.16 from Rattus norvegicus (Rat) (see 2 papers)
36% identity, 99% coverage: 7:615/618 of query aligns to 886:1494/1500 of P07756

query
sites
P07756

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x
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x
N

G

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N

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K

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S1331 (= S453) modified: carbohydrate, O-linked (GlcNAc) serine
T1332 (= T454) modified: carbohydrate, O-linked (GlcNAc) threonine
T1391 (= T515) mutation to V: 400-fold increase in the activation constant of NAG. 3-fold decrease in the reaction rate at saturation of NAG.
T1394 (= T518) mutation to A: 900-fold increase in the activation constant of NAG. 3-fold decrease in the reaction rate at saturation of NAG.
W1410 (≠ K534) mutation to K: 60-fold increase in the activation constant of NAG.
N1437 (= N557) mutation to D: 70-fold increase in the activation constant of NAG.
N1440 (≠ R560) mutation to D: 110-fold increase in the activation constant of NAG. Modifies the specificity for the activator: Binds Phe-NAG considerably better than NAG.

Sites not aligning to the query:

537 modified: carbohydrate, O-linked (GlcNAc) serine; alternate

Q09794 Multifunctional protein ura1; Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase; CPSase-ATCase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
36% identity, 97% coverage: 17:616/618 of query aligns to 937:1535/2244 of Q09794

query
sites
Q09794

S

S

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I

L

A

P

I

Y

A

V

K

K

E

K

L

L

Y

Y

R

E

M

N

D

N

F

Y

D

N

L

I

V

F

A

A

T

T

A

A

G

G

T

T

H

S

R

D

F

Y

L

F

I

M

E

E

N

S

G

G

I

V

E

P

C

C

K

K

K

Y

V

L

F

A

K

D

V

L

G

P

E

A

E

E

G

E

R

A

P

N

N

N

I

E

F

Y

D

S

I

L

I

S

K

A

H

H

-

L

-

A

-

N

G

N

K

M

I

I

D

D

F

M

V

Y

I

I

N

N

T

L

P

P

R

S

G

N

E

N

K

R

A

Y

L

R

H

R

D

P

E

A

E

N

A

Y

I

I

G

S

A

S

A

G

-

Y

-

K

-

S

-

R

-

L

S

A

V

I

L

D

S

Y

N

S

V

V

P

P

F

L

V

V

T

T

T

N

I

V

E

K

A

C

A

A

E

K

A

L

S

M

V

I

Q

E

A

A

I

I

D

-

C

C

I

-

R

R

R

N

Q

L

E

D

F

F

G

S

V

L

K

S

S

T

L

V

Q

D

E

F

Y

Q

S

S

A

S

Y

F

R

Q

S1119 (= S199) modified: Phosphoserine

Sites not aligning to the query:

1881 modified: Phosphoserine
1885 modified: Phosphoserine

6w2jB Cps1 bound to allosteric inhibitor h3b-374 (see paper)
35% identity, 98% coverage: 7:614/618 of query aligns to 775:1364/1364 of 6w2jB

query
sites
6w2jB

S

N

L

M

S

E

Q

K

E

T

L

L

S

K

G

G

L

L

A

N

S

S

K

E

-

S

L

M

P

T

K

E

T

T

L

L

I

K

K

R

A

A

K

K

E

E

H

I

G

G

F

F

S

S

D

D

C

K

Q

Q

L

I

A

S

N

K

I

C

F

L

K
x
G

T

L

T

T

E

E

T

A

V

Q

I

T

R

R

T

E

L

L

R

R

K

L

Q

K

Y

K

G

N

V

I

E

H

S

P

V

W

F

V

K

K

T

Q

V

I

D

D

T

T

C

L

A

A

E

E

F

Y

D

P

A

S

K

V

T

T

P

N

Y

Y

H

L

Y

Y

S

V

T

T

Y

Y

D

N

E

G

E

Q

N

E

E

H

S

D

V

V

R

N

S

F

D

D

R

D

K

H

K

G

V

M

I

M

I

V

L

L

G

G

G

C

G

G

P

P

N

Y

R

H

I

I

G

G

Q

S

G

S

I

V

E

E

F

F

D

D

Y

W

C

C

C

A

V

V

Q

S

A

S

V

I

F

R

A

T

L

L

R

R

E

Q

A

L

G

G

Y

K

E

K

T

T

I

V

M

V

V

V

N

N

C

C

N

N

P

P

E

E

T

T

V

V

S

S

T

T

D

D

Y

F

D

D

I

E

A

C

D

D

K

K

L

L

Y

Y

F

F

E

E

P

E

L

L

T

S

F

L

E

E

D

R

T

I

I

L

R

D

I

I

I

Y

E

H

H

Q

E

E

Q
x
A

P

C

L

G

G

G

V

C

I

I

V

I

S

S

F

V

G

G

Q

Q

T

I

P

P

L

N

K

N

L

L

S

A

T

V

K

P

L

L

D

Y

E

K

A

N

G

G

V

V

T

K

I

I

L

M

G

G

T

T

S

S

S

P

K

L

G

Q

I

I

D

D

L

R

A

A

E

E

D

D

R

R

K

S

K

I

F

F

G

S

A

A

L

V

L

L

E

D

K

E

L

L

D

K

I

V

L

A

H

Q

P

A

D

P

Y

W

G

K

T

A

A

V

I

N

C

T

F

L

D

N

E

E

A

A

L

L

A

E

I

F

T

A

E

K

R

S

I

V

G

D

Y

Y

P

P

V

C

L

L

V

L

R

R

P

P

S

-

Y

-

V

-

L

-

G

-

R

-

A

-

M

M

K

N

I

V

Y

F

N

S

K

E

D

D

S

E

L

M

K

K

E

K

Y

F

V

L

D

E

Q

-

A

-

L

-

F

-

I

-

S

-

E

-

K

-

Y

-

P

P

L

V

I

L

D

T

R

K

F

F

L

V

E

E

T

G

A

A

V

R

E

E

F

V

D

E

I

M

D

D

A

A

I

V

A

G

D
x
K

T

D

T

G

D

R

C

V

V

I

I

S

S

H

G

A

I

I

M

S

Q

E

H

H

V

V

E

E

A

D

A

A

G

G

I

V

H

H

S

S

G

G

D

D

S

A

T

T

S

L

I

M

L

L

P

P

Y

T

R

Q

N

T

I

I

S

S

Q

Q

E

G

V

A

I

I

N

E

T

K

M

V

K

K

A

D

Y

A

T

T

R

R

T

K

L

I

A

A

E

K

H

A

L

F

K

A

V

I

V

S

G

G

L

P

M

F

N

N

V

V

Q

Q

Y

F

A

L

V

V

Q

K

N

G

E

N

S

D

V

V

Y

L

V

V

I

I

E

E

V

C

N

N

P

L

R

R

A

A

S

S

R

R

T

S

V

F

P

P

F

F

V

V

G

S

K

K

A

T

T

L

A

G

V

V

P

D

V

F

V

I

K

D

I

V

A

A

T

T

R

K

V

V

M

M

L

I

G

G

E

E

K

N

L

V

S

D

D

E

L

-

R

-

K

K

E

H

Y

L

D

P

L

T

K

L

D

D

C

H

D

P

E

I

L

I

G

P

M

A

K

D

H

Y

M

V

A

A

I

I

K

K

E

A

P

P

V

M

F

F

P

S

F

W

S

P

K

R

F

L

V
x
R

K

D

S

A

G

D

V

P

Y

I

L

L

G

R

P

C

E

E

M

M

R

A

S

S

T

T

G

G

E

E

A

V

M

A

S

C

L

F

A

G

E

E

Q

G

F

I

P

H

E

T

A

A

F

F

A

L

K

K

A

A

Y

M

Q

L

A

S

A

T

N

G

M

F

E

K

L

I

P

P

L

Q

S

K

G

G

S

-

V

I

F

L

I

I

S

G

V

I

N

Q

D

-

Q

Q

D

S

K

F

S

R

Q

P

R

R

I

F

I

L

A

G

I

V

A

A

K

E

E

Q

L

L

Y

H

R

N

M

E

D

G

F

F

D

K

L

L

V

F

A

A

T

T

A

E

G

A

T

T

H

S

R

D

F

W

L

L

I

N

E

A

N

N

G

N

I

V

E

P

C

A

K

T

K

P

V

V

F

A

K

W

V

P

G

S

E

Q

E

E

G

G

R

Q

-

N

-

P

-

S

-

L

P

S

N

S

I

I

F

R

D

K

I

L

I

I

K

R

H

D

G

G

K

S

I

I

D

D

F

L

V

V

I

I

N

N

T

L

P

P

R

N

G

N

E

N

-

T

K

K

A

F

L

V

H

H

D

D

E

N

E

Y

A

V

I

I

G

R

A

R

A

T

S

A

V

V

L

D

S

S

N

G

V

I

P

P

F

L

V

L

T

T

T

N

I

F

E

Q

A

V

A

T

E

K

A

L

S

F

V

A

Q

E

A

A

I

V

D

Q

C

K

I

S

R

S

R

K

Q

S

E

L

F

F

G

-

V

-

K

-

S

H

L

Y

Q

R

E

Q

Y

Y

S

S

A

A

active site: G811 (≠ K42), A937 (≠ Q168), K1055 (≠ D303), R1191 (≠ V441)

Sites not aligning to the query:

active site: 593, 607, 609, 615
binding (2-fluoranyl-4-methoxy-phenyl)-[(3~{R},5~{R})-4-(2-fluoranyl-4-methoxy-phenyl)carbonyl-3,5-dimethyl-piperazin-1-yl]methanone: 547, 549, 557, 668, 669, 672, 673, 698, 702, 705, 706, 737, 739, 740
binding zinc ion: 273, 293, 295, 320

6w2jA Cps1 bound to allosteric inhibitor h3b-374 (see paper)
35% identity, 98% coverage: 7:614/618 of query aligns to 833:1422/1422 of 6w2jA

query
sites
6w2jA

S

N

L

M

S

E

Q

K

E

T

L

L

S

K

G

G

L

L

A

N

S

S

K

E

-

S

L

M

P

T

K

E

T

T

L

L

I

K

K

R

A

A

K

K

E

E

H

I

G

G

F

F

S

S

D

D

C

K

Q

Q

L

I

A

S

N

K

I

C

F

L

K
x
G

T

L

T

T

E

E

T

A

V

Q

I

T

R

R

T

E

L

L

R

R

K

L

Q

K

Y

K

G

N

V

I

E

H

S

P

V

W

F

V

K

K

T

Q

V

I

D

D

T

T

C

L

A

A

E

E

F

Y

D

P

A

S

K

V

T

T

P

N

Y

Y

H

L

Y

Y

S

V

T

T

Y

Y

D

N

E

G

E

Q

N

E

E

H

S

D

V

V

R

N

S

F

D

D

R

D

K

H

K

G

V

M

I

M

I

V

L

L

G

G

G

C

G

G

P

P

N

Y

R

H

I

I

G

G

Q

S

G

S

I

V

E

E

F

F

D

D

Y

W

C

C

C

A

V

V

Q

S

A

S

V

I

F

R

A

T

L

L

R

R

E

Q

A

L

G

G

Y

K

E

K

T

T

I

V

M

V

V

V

N

N

C

C

N

N

P

P

E

E

T

T

V

V

S

S

T

T

D

D

Y

F

D

D

I

E

A

C

D

D

K

K

L

L

Y

Y

F

F

E

E

P

E

L

L

T

S

F

L

E

E

D

R

T

I

I

L

R

D

I

I

I

Y

E

H

H

Q

E

E

Q
x
A

P

C

L

G

G

G

V

C

I

I

V

I

S

S

F

V

G

G

Q

Q

T

I

P

P

L

N

K

N

L

L

S

A

T

V

K

P

L

L

D

Y

E

K

A

N

G

G

V

V

T

K

I

I

L

M

G

G

T

T

S

S

S

P

K

L

G

Q

I

I

D

D

L

R

A

A

E

E

D

D

R

R

K

S

K

I

F

F

G

S

A

A

L

V

L

L

E

D

K

E

L

L

D

K

I

V

L

A

H

Q

P

A

D

P

Y

W

G

K

T

A

A

V

I

N

C

T

F

L

D

N

E

E

A

A

L

L

A

E

I

F

T

A

E

K

R

S

I

V

G

D

Y

Y

P

P

V

C

L

L

V

L

R

R

P

P

S

-

Y

-

V

-

L

-

G

-

R

-

A

-

M

M

K

N

I

V

Y

F

N

S

K

E

D

D

S

E

L

M

K

K

E

K

Y

F

V

L

D

E

Q

-

A

-

L

-

F

-

I

-

S

-

E

-

K

-

Y

-

P

P

L

V

I

L

D

T

R

K

F

F

L

V

E

E

T

G

A

A

V

R

E

E

F

V

D

E

I

M

D

D

A

A

I

V

A

G

D
x
K

T

D

T

G

D

R

C

V

V

I

I

S

S

H

G

A

I

I

M

S

Q

E

H

H

V

V

E

E

A

D

A

A

G

G

I

V

H

H

S

S

G

G

D

D

S

A

T

T

S

L

I

M

L

L

P

P

Y

T

R

Q

N

T

I

I

S

S

Q

Q

E

G

V

A

I

I

N

E

T

K

M

V

K

K

A

D

Y

A

T

T

R

R

T

K

L

I

A

A

E

K

H

A

L

F

K

A

V

I

V

S

G

G

L

P

M

F

N

N

V

V

Q

Q

Y

F

A

L

V

V

Q

K

N

G

E

N

S

D

V

V

Y

L

V

V

I

I

E

E

V

C

N

N

P

L

R

R

A

A

S

S

R

R

T

S

V

F

P

P

F

F

V

V

G

S

K

K

A

T

T

L

A

G

V

V

P

D

V

F

V

I

K

D

I

V

A

A

T

T

R

K

V

V

M

M

L

I

G

G

E

E

K

N

L

V

S

D

D

E

L

-

R

-

K

K

E

H

Y

L

D

P

L

T

K

L

D

D

C

H

D

P

E

I

L

I

G

P

M

A

K

D

H

Y

M

V

A

A

I

I

K

K

E

A

P

P

V

M

F

F

P

S

F

W

S

P

K

R

F

L

V
x
R

K

D

S

A

G

D

V

P

Y

I

L

L

G

R

P

C

E

E

M

M

R

A

S

S

T

T

G

G

E

E

A

V

M

A

S

C

L

F

A

G

E

E

Q

G

F

I

P

H

E

T

A

A

F

F

A

L

K

K

A

A

Y

M

Q

L

A

S

A

T

N

G

M

F

E

K

L

I

P

P

L

Q

S

K

G

G

S

-

V

I

F

L

I

I

S

G

V

I

N

Q

D

-

Q

Q

D

S

K

F

S

R

Q

P

R

R

I

F

I

L

A

G

I

V

A

A

K

E

E

Q

L

L

Y

H

R

N

M

E

D

G

F

F

D

K

L

L

V

F

A

A

T

T

A

E

G

A

T

T

H

S

R

D

F

W

L

L

I

N

E

A

N

N

G

N

I

V

E

P

C

A

K

T

K

P

V

V

F

A

K

W

V

P

G

S

E

Q

E

E

G

G

R

Q

-

N

-

P

-

S

-

L

P

S

N

S

I

I

F

R

D

K

I

L

I

I

K

R

H

D

G

G

K

S

I

I

D

D

F

L

V

V

I

I

N

N

T

L

P

P

R

N

G

N

E

N

-

T

K

K

A

F

L

V

H

H

D

D

E

N

E

Y

A

V

I

I

G

R

A

R

A

T

S

A

V

V

L

D

S

S

N

G

V

I

P

P

F

L

V

L

T

T

T

N

I

F

E

Q

A

V

A

T

E

K

A

L

S

F

V

A

Q

E

A

A

I

V

D

Q

C

K

I

S

R

S

R

K

Q

S

E

L

F

F

G

-

V

-

K

-

S

H

L

Y

Q

R

E

Q

Y

Y

S

S

A

A

active site: G869 (≠ K42), A995 (≠ Q168), K1113 (≠ D303), R1249 (≠ V441)

Sites not aligning to the query:

active site: 651, 665, 667, 673
binding (2-fluoranyl-4-methoxy-phenyl)-[(3~{R},5~{R})-4-(2-fluoranyl-4-methoxy-phenyl)carbonyl-3,5-dimethyl-piperazin-1-yl]methanone: 605, 607, 615, 725, 726, 727, 730, 732, 756, 760, 763, 764, 795, 797, 798
binding zinc ion: 273, 293, 295, 320

5douD Crystal structure of human carbamoyl phosphate synthetase i (cps1), ligand-bound form (see paper)
35% identity, 95% coverage: 7:596/618 of query aligns to 844:1418/1430 of 5douD

query
sites
5douD

S

N

L

M

S

E

Q

K

E

T

L

L

S

K

G

G

L

L

A

N

S

S

K

E

-

S

L

M

P

T

K

E

T

T

L

L

I

K

K

R

A

A

K

K

E

E

H

I

G

G

F

F

S

S

D

D

C

K

Q

Q

L

I

A

S

N

K

I

C

F

L

K
x
G

T

L

T

T

E

E

T

A

V

Q

I

T

R

R

T

E

L

L

R

R

K

L

Q

K

Y

K

G

N

V

I

E

H

S

P

V

W

F

V

K

K

T

Q

V

I

D

D

T

T

C

L

A

A

E

E

F

Y

D

P

A

S

K

V

T

T

P

N

Y

Y

H

L

Y

Y

S

V

T

T

Y

Y

D

N

E

G

E

Q

N

E

E

H

S

D

V

V

R

N

S

F

D

D

R

D

K

H

K

G

V

M

I

M

I

V

L

L

G

G

G

C

G

G

P

P

N

Y

R

H

I

I

G

G

Q

S

G

S

I

V

E

E

F

F

D

D

Y

W

C

C

C

A

V

V

Q

S

A

S

V

I

F

R

A

T

L

L

R

R

E

Q

A

L

G

G

Y

K

E

K

T

T

I

V

M

V

V

V

N

N

C

C

N

N

P

P

E

E

T

T

V

V

S

S

T

T

D

D

Y

F

D

D

I

E

A

C

D

D

K

K

L

L

Y

Y

F

F

E

E

P

E

L

L

T

S

F

L

E

E

D

R

T

I

I

L

R

D

I

I

I

Y

E

H

H

Q

E

E

Q
x
A

P

C

L

G

G

G

V

C

I

I

V

I

S

S

F

V

G

G

Q

Q

T

I

P

P

L

N

K

N

L

L

S

A

T

V

K

P

L

L

D

Y

E

K

A

N

G

G

V

V

T

K

I

I

L

M

G

G

T

T

S

S

S

P

K

L

G

Q

I

I

D

D

L

R

A

A

E

E

D

D

R

R

K

S

K

I

F

F

G

S

A

A

L

V

L

L

E

D

K

E

L

L

D

K

I

V

L

A

H

Q

P

A

D

P

Y

W

G

K

T

A

A

V

I

N

C

T

F

L

D

N

E

E

A

A

L

L

A

E

I

F

T

A

E

K

R

S

I

V

G

D

Y

Y

P

P

V

C

L
|
L

V

L

R
|
R

P

-

S

-

Y

-

V

-

L

-

G

-

R

-

A

-

M

M

K

N

I

V

Y

F

N

S

K

E

D

D

S

E

L

M

K

K

E

K

Y

F

V

L

D

E

Q

-

A

-

L

-

F

-

I

-

S

-

E

-

K

-

Y

H

P

P

L

V

I

L

D

T

R

K

F
|
F

L
x
V

E

E

T

G

A

A

V

R

E
|
E

F

V

D

E

I

M

D

D

A

A

I

V

A

G

D
x
K

T

D

T

G

D

R

C

V

V

I

I

S

S

H

G

A

I

I

M

S

Q

E

H

H

V

V

E

E

A

D

A
|
A

G

G

I
x
V

H
|
H

S
|
S

G

G

D

D

S

A

T

T

S

L

I

M

L

L

P

P

Y

T

R

Q

N

T

I

I

S

S

Q

Q

E

G

V

A

I

I

N

E

T

K

M

V

K

K

A

D

Y

A

T

T

R

R

T

K

L

I

A

A

E

K

H

A

L

F

K

A

V

I

V

S

G

G

L

P

M

F

N

N

V

V

Q
|
Q

Y

F

A
x
L

V

V

Q

K

N

G

E

N

S

D

V

V

Y

L

V

V

I
|
I

E
|
E

V

C

N
|
N

P

L

R

R

A

A

S

S

R
|
R

T

S

V

F

P

P

F

F

V

V

G

S

K

K

A

T

T

L

A

G

V

V

P

D

V

F

V

I

K

D

I

V

A

A

T

T

R

K

V

V

M

M

L

I

G

G

E

E

K

N

L

V

S

D

D

E

L

-

R

-

K

K

E

H

Y

L

D

P

L

T

K

L

D

D

C

H

D

P

E

I

L

I

G

P

M

A

K

D

H

Y

M

V

A

A

I

I

K

K

E

A

P

P

V

M

F

F

P

S

F

W

S

P

K

R

F

L

V
x
R

K

D

S

A

G

D

V

P

Y

I

L

L

G

R

P

C

E

E

M

M

R

A

S

S

T

T

G

G

E

E

A

V

M

A

S

C

L

F

A

G

E

E

Q

G

F

I

P

H

E

T

A

A

F

F

A

L

K

K

A

A

Y

M

Q

L

A

S

A

T

N

G

M

F

E

K

L

I

P

P

L

Q

S

K

G

G

S

-

V

I

F

L

I

I

S

G

V
x
I

N
x
Q

D

-

Q

Q

D

S

K

F

S

R

Q

P

R

R

I

F

I

L

A

G

I

V

A

A

K

E

E

Q

L

L

Y

H

R

N

M

E

D

G

F

F

D

K

L

L

V

F

A

A

T
|
T

A

E

G
x
A

T
|
T

H

S

R

D

F

W

L

L

I

N

E

A

N

N

G

N

I

V

E

P

C

A

K

T

K

P

V

V

F

A

K
x
W

V

P

G

S

E

Q

E

E

G

G

R

Q

-

N

-

P

-

S

-

L

P

S

N

S

I

I

F

R

D

K

I

L

I

I

K

R

H

D

G

G

K

S

I

I

D

D

F

L

V

V

I

I

N

N

T
x
L

P

P

R

N

G

N

E

N

-
x
T

K
|
K

A
x
F

L

V

H

H

D

D

E
x
N

E

Y

A

V

I

I

G

R

A

R

A

T

S

A

V

V

L

D

S

S

N

G

V

I

P

P

F

L

V

L

T

T

T

N

I

F

E

Q

A

V

A

T

E

K

A

L

S

F

V

A

Q

E

A

A

I

V

active site: G880 (≠ K42), A1006 (≠ Q168), R1087 (= R249), E1116 (= E295), K1124 (≠ D303), Q1184 (= Q363), E1196 (= E375), N1198 (= N377), R1203 (= R382), R1260 (≠ V441)
binding adenosine-5'-diphosphate: L1085 (= L247), F1110 (= F289), V1111 (≠ L290), E1116 (= E295), A1140 (= A319), V1142 (≠ I321), H1143 (= H322), S1144 (= S323), Q1184 (= Q363), L1186 (≠ A365), I1195 (= I374), E1196 (= E375)
binding n-acetyl-l-glutamate: I1307 (≠ V489), Q1308 (≠ N490), T1332 (= T515), A1334 (≠ G517), T1335 (= T518), W1351 (≠ K534), L1379 (≠ T558), T1384 (vs. gap), K1385 (= K563), F1386 (≠ A564), N1390 (≠ E568)

Sites not aligning to the query:

active site: 252, 335, 337, 505, 545, 576, 589, 617, 656, 658, 672, 674, 676, 680
binding adenosine-5'-diphosphate: 505, 543, 545, 550, 551, 552, 581, 583, 584, 585, 589, 614, 615, 616, 617, 658, 671, 672
binding magnesium ion: 658, 672, 672, 674
binding phosphate ion: 550, 551, 617, 672, 674, 676, 679

P05990 Multifunctional protein r; Protein rudimentary; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Drosophila melanogaster (Fruit fly) (see 2 papers)
36% identity, 89% coverage: 8:559/618 of query aligns to 861:1411/2224 of P05990

query
sites
P05990

L

L

S

Q

Q

S

E

L

L

L

S

T

G

R

L

N

A

G

S

S

K

R

L

T

P

D

K

A

T

L

L

L

I

L

K

K

A

A

K

K

E

R

H

F

G

G

F

F

S

S

D

D

C

K

Q

Q

L

I

A

A

N

K

I

Y

F

I

K

K

T

S

T

T

E

E

T

L

V

A

I

V

R

R

T

H

L

Q

R

R

K

Q

Q

E

Y

F

G

G

V

I

E

R

S

P

V

H

F

V

K

K

T

Q

V

I

D

D

T

T

C

V

A

A

A

G

E

E

F

W

D

P

A

A

K

S

T

T

P

N

Y

Y

H

L

Y

Y

S

H

T

T

Y

Y

D

N

E

G

E

S

N

E

E

H

S

D

V

V

R

D

S

F

D

P

R

G

K

G

K

H

V

T

I

I

I

V

L

V

G

G

G

S

G

G

P

V

N

Y

R

R

I

I

G

G

Q

S

G

S

I

V

E

E

F

F

D

D

Y

W

C

C

C

A

V

V

Q

G

A

C

V

L

F

R

A

E

L

L

R

R

E

K

A

L

G

Q

Y

R

E

P

T

T

I

I

M

M

V

I

N

N

C

Y

N

N

P

P

E

E

T

T

V

V

S

S

T

T

D

D

Y

Y

D

D

I

M

A

C

D

D

K

R

L

L

Y

Y

F

F

E

E

P

E

L

I

T

S

F

F

E

E

D

V

T

V

I

M

R

D

I

I

I

Y

E

E

H

M

E

E

Q

N

P

S

L

E

G

G

V

I

I

I

V

L

S

S

F

M

G

G

Q

Q

T

L

P

P

L

N

K

N

L

I

S

A

T

M

K

D

L

L

D

H

E

R

A

Q

G

Q

V

A

T

K

I

V

L

L

G

G

T

T

S

S

S

P

K

E

G

S

I

I

D

D

L

C

A

A

E

E

D

N

R

R

K

F

K

K

F

F

G

S

A

R

L

M

L

L

E

D

K

R

L

K

D

G

I

I

L

L

H

Q

P

P

D

R

Y

W

G

K

T

E

A

L

I

T

C

N

F

L

D

Q

E

S

A

A

L

I

A

E

I

F

T

C

E

E

R

E

I

V

G

G

Y

Y

P

P

V

C

L

L

V

V

R

R

P

P

S

S

Y

Y

V

V

L

L

G

S

G

G

R

A

A

A

M

M

K

N

I

V

I

A

Y

Y

N

S

K

N

D

Q

S

D

L

L

K

E

E

T

Y

Y

V

L

D

N

Q

A

A

A

L

S

F

E

I

V

S

S

E

R

K

E

Y

H

P

P

L

V

I

I

D

S

R

K

F

F

L

L

E

T

T

E

A

A

V

K

E

E

F

I

D

D

I

V

D

D

A

A

I

V

A

A

D

S

T

D

T

G

D

R

C

I

V

L

I

C

S

M

G

A

I

V

M

S

Q
x
E

H

H

V

V

E

E

A

N

A

A

G

G

I

V

H

H

S

S

G

G

D

D

S

A

T

T

S

L

I

V

L

T

P

P

Y

P

R

Q

N

D

I

L

S

N

Q

A

E

E

V

T

I

L

N

E

T

A

M

I

K

K

A

R

Y

I

T

T

R

C

T

D

L

L

A

A

E

S

H

V

L

L

K

D

V

V

V

T

G

G

L

P

M

F

N

N

V

M

Q

Q

Y

L

A

I

V

A

Q

K

N

N

E

N

S

E

V

L

Y

K

V

V

I

I

E

E

V

C

N

N

P

V

R

R

A

V

S

S

R

R

T

S

V

F

P

P

F

F

V

V

G

S

K

K

A

T

T

L

A

D

V

H

P

D

V

F

V

V

K

A

I

T

A

A

T

T

R

R

V

A

M

I

L

V

G

G

E

-

K

-

L

-

S

-

D

-

L

-

R

-

K

-

E

-

Y

L

D

D

L

V

K

E

D

P

C

L

D

D

E

V

L

L

-

H

G

G

M

V

K

G

H

K

M

V

A

G

I

V

K

K

E

V

P

P

V

Q

F

F

P

S

F

F

S

S

K

R

F

L

V

A

K

G

S

A

G

D

V

V

Y

Q

L

L

G

G

P

V

E

E

M

M

R

A

S

S

T

T

G

G

E

E

A

V

M

A

S

C

L

F

A

G

E

D

Q

N

F

R

P

Y

E

E

A

A

F

Y

A

L

K

K

A

A

Y

M

Q

M

A

S

A

T

N

G

M

F

E

Q

L

I

P

P

L

-

S

K

G

N

S

A

V

V

F

L

I

L

S

S

V

I

N

G

D

S

Q

F

D

K

K

H

S

K

Q

M

R

E

I

L

A

P

I

S

A

I

K

R

E

D

L

L

Y

A

R

K

M

M

D

G

F

Y

D

K

L

L

V

Y

A

A

T

S

A

M

G

G

T

T

H

G

R

D

F

F

L

Y

I

A

E

E

N

H

G

G

I

V

E

N

C

V

K

E

K

S

V

V

F

Q

K

W

V

T

G

F

E

D

E

K

G

T

R

T

P

P

N

D

-

D

-

I

-

N

-

G

-

E

-

L

-

R

-

H

I

L

F

A

D

E

I

F

I

L

K

A

H

N

G

K

K

Q

I

F

D

D

F

L

V

V

I

I

N

N

T

L

P

P

E1167 (≠ Q314) mutation to K: Severely diminishes UTP inhibition of CPSase; in Su(b).

Sites not aligning to the query:

1883 modified: Phosphoserine
1885 modified: Phosphoserine
1892 modified: Phosphoserine
1894 modified: Phosphoserine

Q18990 Multifunctional protein pyr-1; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Caenorhabditis elegans (see 3 papers)
34% identity, 97% coverage: 3:602/618 of query aligns to 854:1458/2198 of Q18990

query
sites
Q18990

T

T

Q

D

V

V

S

N

S

T

L

V

S

S

Q

A

E

E

L

L

S

-

G

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A

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-

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-

T

-

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L

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L

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A

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V

A

A

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G

E

E

F

W

D

P

A

A

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Q

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Y

H

L

Y

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T

T

Y

F

D

N

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-

I

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F

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N

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M

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K

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L

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A

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P

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A

A

G

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G

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N

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K

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K

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K

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A

A

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T

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A

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M

F

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N

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N

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N

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L

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M

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L

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A

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D

A

A

F

Y

A

L

K

K

A

A

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L

Q

L

A

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A

T

N

G

M

F

E

V

L

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P

P

L

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S

K

G

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N

V

I

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F

I

I

S

S

V

I

N

G

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Y

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Q

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E

I

M

I

L

A

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A

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K

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A

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L

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M

L

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S

A

K

G

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N

N

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P

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W

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N

D

-

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-

K

-

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-

A

-

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-

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-

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-

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-

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F

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L

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E

H

N

G

K

K

E

I

F

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H

F

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I

I

N

N

T

L

P

P

-

I

R

R

G

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K

G

A

A

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Y

-

R

-

V

-

S

-

A

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F

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-

T

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E

K

A

T

I

R

G

R

A

M

A

A

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-

V

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S

N

N

G

V

I

P

P

F

L

V

I

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D

I

I

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K

A

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A

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M

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G

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Q

R

Sites not aligning to the query:

1602 H→Q: In cu8; probable loss of dihydroorotase activity. Severe late stage embryonic lethality in 50% of animals. The few surviving mutants have a shorter and thicker pharyngeal isthmus, an abnormal knobbed tail, mild egg-laying defects, moderate fluid accumulation in the coelom and a slower growth. Actin and intermediate filaments are disorganized in the pharynx. Moderate reduction in heparan sulfate levels and increased levels of chondroitin sulfate. In an umps-1 zu456 mutant background, prevents the formation of abnormally enlarged gut granules in embryos. Complete embryonic lethality in a rnst-2 qx245 mutant background.

P08955 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Mesocricetus auratus (Golden hamster) (see paper)
36% identity, 87% coverage: 19:557/618 of query aligns to 858:1394/2225 of P08955

query
sites
P08955

L

L

P

S

K

Q

K

D

T

L

L

L

I

H

K

Q

A

A

K

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G

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D

C

K

Q

Q

L

I

A

A

N

L

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A

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T

T

E

E

T

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A

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T

K

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K

Q

Q

E

Y

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A

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A

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T

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P

N

Y

Y

H

L

Y

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S

L

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Y

Y

D

W

E

G

E

N

N

T

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H

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K

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I

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Y

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I

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C

C

C

A

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A

M

G

G

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Y

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M

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N

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Y

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N

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N

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A

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G

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Y

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L

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A

A

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T

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A

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I

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D

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D

D

A

A

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A

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-

T

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T

C

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C

G

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A

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A

-

I

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A

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D

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N

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A

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A

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A

A

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M

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G

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E

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L

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R

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E

-

Y

-

D

-

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C

-

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-

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M

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G

G

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A

G

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A

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D

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A

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A

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M

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L

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A

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N

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L

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S

K

G

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S

N

V

I

F

L

I

L

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T

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I

N

G

D

S

Q

Y

D

K

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N

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I

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I

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A

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K

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E

L

L

L

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S

M

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Y

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S

L

L

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Y

A

A

T

S

A

L

G

G

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T

H

A

R

D

F

F

L

Y

I

T

E

E

N

H

G

G

I

V

E

K

C

V

K

T

K

A

V

V

F

D

K

W

V

H

G

F

E

E

G

A

-

V

-

D

-

G

-

E

-

C

-

P

R

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P

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N

S

I

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Q

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A

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N

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V

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I

N

N

Sites not aligning to the query:

1406 modified: Phosphoserine; by PKA; S→A: No effect on enzyme kinetics.; S→D: Increases CPSase activity and reduces sensitivity to feedback inhibition by UTP.

P27708 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Homo sapiens (Human) (see 7 papers)
37% identity, 83% coverage: 19:532/618 of query aligns to 858:1362/2225 of P27708

query
sites
P27708

L

L

P

P

K

P

K

D

T

L

L

L

I

Q

K

Q

A

A

K

K

E

C

H

L

G

G

F

F

S

S

D

D

C

K

Q

Q

L

I

A

A

N

L

I

A

F

V

K

L

T

S

T

T

E

E

T

L

V

A

I

V

R

R

T

K

L

L

R

R

K

Q

Q

E

Y

L

G

G

V

I

E

C

S

P

V

A

F

V

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K

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Q

V

I

D

D

T

T

C

V

A

A

E

E

F

W

D

P

A

A

K

Q

T

T

P

N

Y

Y

H

L

Y

Y

S

L

T

T

Y

Y

D

W

E

G

E

T

N

T

E

H

S

D

V

L

R

T

S

F

D

R

R

T

K

P

K

H

V

V

I

L

I

V

L

L

G

G

G

S

G

G

P

V

N

Y

R

R

I

I

G

G

Q

S

G

S

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V

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E

F

F

D

D

Y

W

C

C

C

A

V

V

Q

G

A

C

V

I

F

Q

A

Q

L

L

R

R

E

K

A

M

G

G

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
33 M → R: in DEE50; uncertain significance; dbSNP:rs751610198
177 R → Q: in a colorectal cancer sample; somatic mutation; dbSNP:rs374122292
456 modified: Phosphothreonine; by MAPK1
735 Y → C: in a colorectal cancer sample; somatic mutation
1406 modified: Phosphoserine; by PKA
1471 binding Zn(2+); binding Zn(2+); H→A: No zinc-binding and no catalytic activity.; H→N: Abolishes dihydroorotase activity.
1473 binding Zn(2+); H→A: No zinc-binding and no catalytic activity.
1475 binding (S)-dihydroorotate
1505 binding (S)-dihydroorotate
1512 D→N: No change in catalytic activity.
1556 binding via carbamate group; binding via carbamate group; modified: N6-carboxylysine
1562 T→A: Abolishes dihydroorotase activity.
1563 F→A: Abolishes dihydroorotase activity.
1590 binding Zn(2+); H→A: Abolishes dihydroorotase activity.; H→N: No catalytic activity.
1613 binding Zn(2+); C→S: Reduces dihydroorotase activity.
1614 binding Zn(2+); H→A: Abolishes dihydroorotase activity.
1637 binding Zn(2+); E→T: Abolishes dihydroorotase activity.
1642 H→N: 11.5% of wild-type catalytic activity.
1661 binding (S)-dihydroorotate
1686 binding Zn(2+); D→N: Abolishes dihydroorotase activity.
1690 binding (S)-dihydroorotate; H→N: 3% of wild-type catalytic activity.
1702 binding (S)-dihydroorotate
1789:2225 natural variant: Missing (in DEE50; uncertain significance)
1859 modified: Phosphoserine; by RPS6KB1 and PKA
1873 modified: Phosphoserine; by PKC; in vitro; S→A: Abolishes PMA-induced Thr-456 phosphorylation.
1900 modified: Phosphoserine

P20054 Multifunctional protein pyr1-3; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Dictyostelium discoideum (Social amoeba)
34% identity, 94% coverage: 17:600/618 of query aligns to 870:1453/2225 of P20054

query
sites
P20054

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Sites not aligning to the query:

1 modified: N-acetylmethionine

Query Sequence

>WP_012466868.1 NCBI__GCF_000020465.1:WP_012466868.1
MTTQVSSLSQELSGLASKLPKKTLIKAKEHGFSDCQLANIFKTTETVIRTLRKQYGVESV
FKTVDTCAAEFDAKTPYHYSTYDEENESVRSDRKKVIILGGGPNRIGQGIEFDYCCVQAV
FALREAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDTIRIIEHEQPLGVIVSFGGQT
PLKLSTKLDEAGVTILGTSSKGIDLAEDRKKFGALLEKLDILHPDYGTAICFDEALAITE
RIGYPVLVRPSYVLGGRAMKIIYNKDSLKEYVDQALFISEKYPLLIDRFLETAVEFDIDA
IADTTDCVISGIMQHVEAAGIHSGDSTSILPYRNISQEVINTMKAYTRTLAEHLKVVGLM
NVQYAVQNESVYVIEVNPRASRTVPFVGKATAVPVVKIATRVMLGEKLSDLRKEYDLKDC
DELGMKHMAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLAEQFPEAFAKAYQAANMELP
LSGSVFISVNDQDKSQRIIAIAKELYRMDFDLVATAGTHRFLIENGIECKKVFKVGEEGR
PNIFDIIKHGKIDFVINTPRGEKALHDEEAIGAASVLSNVPFVTTIEAAEASVQAIDCIR
RQEFGVKSLQEYSAYRNK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory