SitesBLAST

P9WP51 Probable cystathionine beta-synthase Rv1077; Beta-thionase; Serine sulfhydrase; EC 4.2.1.22 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 97% coverage: 13:456/457 of query aligns to 14:459/464 of P9WP51

query
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K428 (≠ D424) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8s5hA Full-length human cystathionine beta-synthase with c-terminal 6xhis- tag, basal state, helical reconstruction (see paper)
31% identity, 94% coverage: 5:435/457 of query aligns to 38:471/507 of 8s5hA

query
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8s5hA

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binding protoporphyrin ix containing fe: A185 (≠ P150), Y192 (≠ S157), R225 (≠ H190)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 10, 11, 17, 22, 23, 24

7qgtB Crystal structure of human cystathionine beta-synthase (delta516-525) in complex with aoaa. (see paper)
31% identity, 94% coverage: 5:435/457 of query aligns to 39:472/500 of 7qgtB

query
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7qgtB

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binding protoporphyrin ix containing fe: A186 (≠ P150), P189 (≠ R153), L190 (≠ D154), Y193 (≠ S157), R226 (≠ H190)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: K79 (≠ Y43), T106 (= T70), S107 (≠ Y71), N109 (= N73), T110 (≠ S74), Q182 (≠ L146), G216 (≠ I180), T217 (≠ S181), G218 (= G182), T220 (≠ M184), G265 (≠ E230), S309 (≠ A274), P335 (≠ N300), D336 (= D301)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 10, 11, 12, 13, 14, 23, 24, 25

P35520 Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase; EC 4.2.1.22 from Homo sapiens (Human) (see 40 papers)
31% identity, 94% coverage: 5:435/457 of query aligns to 79:512/551 of P35520

query
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P35520

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G85 (≠ T11) to R: in CBSD; loss of cystathionine beta-synthase activity; dbSNP:rs863223435
T87 (= T13) to N: in CBSD; decreased cystathionine beta-synthase activity; increased aggregation
L101 (= L25) to P: in CBSD; common mutation in Irish population; loss of activity; dbSNP:rs786204757
K102 (= K26) to N: in CBSD; associated in cis with R-78; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204609; to Q: in dbSNP:rs34040148
C109 (≠ L33) to R: in CBSD; loss of activity; dbSNP:rs778220779
A114 (≠ P38) to V: in CBSD; mild form; when linked with W-58 severe form; decreased cystathionine beta-synthase activity; decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs121964964
K119 (≠ Y43) modified: N6-(pyridoxal phosphate)lysine
R125 (≠ A49) to Q: in CBSD; severe form; when linked with D-131 moderate form; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs781444670; to W: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs886057100
M126 (≠ V50) to V: in CBSD; loss of activity
E131 (= E55) to D: in CBSD; linked with Q-125; loss of activity; dbSNP:rs1555875351
G139 (= G63) to R: in CBSD; mild form; dbSNP:rs121964965
I143 (≠ V67) to M: in CBSD; 4% of activity; stable; dbSNP:rs370167302
E144 (≠ D68) to K: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964966
G148 (= G72) to R: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs755952006
N149 (= N73) binding pyridoxal 5'-phosphate
L154 (= L78) to Q: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
A155 (= A79) to T: in CBSD; complete loss of activity; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs1429138569; to V: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
C165 (≠ L89) to Y: in CBSD; severe form; protein expression is comparable to wild-type; loss of cystathionine beta-synthase activity; no effect on homotetramer formation; dbSNP:rs1347651454
M173 (≠ I97) to V: in CBSD; presents 40% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; natural variant: Missing (in CBSD; loss of activity)
E176 (= E100) to K: in CBSD; severe form; loss of cystathionine beta-synthase activity; inhibited by AdoMet; severely decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs762065361
V180 (= V104) to A: in CBSD; decreased cystathionine beta-synthase activity; decreases homotetramer formation; dbSNP:rs1555875010
T191 (= T115) to M: in CBSD; moderate and severe forms; loss of cystathionine beta-synthase activity; absent capacity to form multimeric quaternary structure; dbSNP:rs121964973
K211 (≠ V135) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
A226 (≠ P150) to T: in CBSD; presents 20% of the wild-type activity; dramatically reduced capacity to form multimeric quaternary structure; dbSNP:rs763835246
N228 (≠ S152) to K: in CBSD; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs1464223176; to S: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1555874803
A231 (≠ V155) to P: in CBSD; has significantly decreased levels of enzyme activity
D234 (≠ N158) to N: in CBSD; decreased cystathionine beta-synthase activity; changed localization; decreased interaction with pyridoxal 5'-phosphate; no effect on homotetramer formation; dbSNP:rs773734233; natural variant: Missing (in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity)
GTGGT 256:260 (≠ ISGAM 180:184) binding pyridoxal 5'-phosphate
T257 (≠ S181) to M: in CBSD; moderate to severe form; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs758236584
T262 (≠ S186) to M: in CBSD; moderate form; dbSNP:rs149119723; to R: in CBSD; severe form; loss of cystathionine beta-synthase activity; loss of homotetramer formation
R266 (≠ H190) to K: in CBSD; mild form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; no effect on heme-binding; decreased stability; dbSNP:rs121964969
K269 (= K193) natural variant: Missing (in CBSD; loss of expression)
C272 (≠ K196) mutation to A: Reduced heme content and cystathionine beta-synthase activity.
C275 (≠ I199) to Y: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; mutation to S: Reduced heme content and cystathionine beta-synthase activity.
I278 (= I202) to S: in CBSD; loss of activity; to T: in CBSD; mild to severe form; common mutation; decreased expression; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs5742905
D281 (≠ E205) to N: in CBSD; loss of activity
A288 (≠ Q212) to T: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs141502207
E302 (= E227) to K: in CBSD; no effect on cystathionine beta-synthase activity; inhibited by AdoHcy and impaired activation by AdoMet; no effect on homotetramer formation; dbSNP:rs779270933
G305 (≠ E230) to R: in CBSD; loss of cystathionine beta-synthase activity; no effect on homotetramer formation
G307 (= G232) to S: in CBSD; moderate to severe form; linked with D-534; common mutation; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; no effect on homotetramer formation; dbSNP:rs121964962
V320 (≠ I245) to A: in CBSD; has 36% of wild-type enzyme activity; dbSNP:rs781567152
D321 (= D246) to V: in CBSD; loss of activity
R336 (= R261) to C: in CBSD; protein expression is comparable to wild-type; loss of activity; absent capacity to form multimeric quaternary structure; dbSNP:rs398123151; to H: in CBSD; mild form; no effect on expression; exhibits an activity lower than 4% of the wild-type enzyme; altered stimulation by AdoMet; absent capacity to form multimeric quaternary structure; dbSNP:rs760417941
L338 (= L263) to P: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
G347 (= G272) to S: in CBSD; protein expression is comparable to wild-type; loss of activity; dbSNP:rs771298943
S349 (≠ A274) binding pyridoxal 5'-phosphate; to N: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
T353 (≠ V278) to M: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs121964972
R369 (≠ C294) to C: in CBSD; when linked with C-491 severe form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs117687681
D376 (= D301) to N: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1170128038
R379 (≠ G304) to Q: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs763036586
K384 (= K309) to E: in CBSD; severe form; dbSNP:rs121964967
P422 (vs. gap) to L: in CBSD; changed cystathionine beta-synthase activity; impaired stimulation by AdoMet; does not affect homotetramer formation; dbSNP:rs28934892
P427 (= P351) to L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs863223434
I435 (≠ F359) to T: in CBSD; no effect on cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; does not affect homotetramer formation
R439 (≠ K363) to Q: in CBSD; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs756467921
D444 (≠ S368) to N: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; increased homotetramer formation; dbSNP:rs28934891
V449 (= V373) to G: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
L456 (≠ I379) to P: in CBSD; severe; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
S466 (= S389) to L: in CBSD; increased cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964971
S500 (= S423) to L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs755106884

Sites not aligning to the query:

18 R → C: results in 1/3 to 2/3 the enzyme activity of the wild-type; dbSNP:rs201827340
49 P → L: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs148865119
52 binding axial binding residue
58 R → W: in CBSD; linked with V-114; 18% of activity; dbSNP:rs555959266
65 binding axial binding residue; H → R: in CBSD; decreased cystathionine beta-synthase activity; inhibited by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs1191141364
69 A → P: in dbSNP:rs17849313
78 P → R: in CBSD; severe form; associated in cis with N-102; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204608
526 Q → K: in CBSD; has significantly decreased levels of enzyme activity
539 L → S: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs121964968
540 L → Q: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
548 R → Q: presents 60% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; dbSNP:rs150828989

4pcuA Crystal structure of delta516-525 e201s human cystathionine beta- synthase with adomet (see paper)
31% identity, 94% coverage: 5:435/457 of query aligns to 37:466/486 of 4pcuA

query
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G

Q

E

E

N

G

D

Q

C

R

I

C

V

V

A

V

V

I

L

L

N
x
P

D
|
D

F

S

G

V

G

R

Y

N

Y

Y

L

M

S

T

K

K

M

F

F

L

R

S

D

D

G

R

W

W

M

M

K

L

Q

Q

K

K

G

G

Y

F

Y

L

R

K

K

E

A

E

K

D

T

L

A

K

L

K

E

P

Q

W

I

W

T

W

A

H

E

L

D

R

I

V

L

Q

Q

E

L

L

K

G

A

L

R

S

K

A

D
x
P

L

L

I

T

F

V

A

L

N

-

P

P

E

T

A

I

T

T

L

C

A

G

E

H

V

T

F

I

E

E

M

I

M

L

K

R

Q

E

N

K

D
x
G

V
x
F

S
x
D

Q
|
Q

L

A

P

P

I

V

V

V

-

D

S

E

Y

A

G

G

T

V

P

I

I

L

G

G

S

M

I

V

S

T

E

L

N

G

K

N

I

M

L

L

S

S

I

S

L

L

I

L

E

A

N

G

D

K

E

V

A

Q

M

P

N

S

S

D

K

Q

V

V

V

G

G

K

F

V

M

I

E

Y

P

K

P

Q

F

F

P

K

V

Q

C

I

Q

R

T

L

D

T

A

D

T

T

I

L

S

G

E

R

L

L

S

S

D

H

K

I

L

L

Q

E

Q

M

S

D

A

H

S

F

G

A

I

L

L

V

I

V

active site: K77 (≠ Y43), S105 (≠ Y71), D237 (≠ E205), S305 (≠ A274)
binding protoporphyrin ix containing fe: A182 (≠ P150), P185 (≠ R153), L186 (≠ D154), Y189 (≠ S157), R222 (≠ H190), T269 (≠ G238)
binding pyridoxal-5'-phosphate: K77 (≠ Y43), N107 (= N73), G212 (≠ I180), T213 (≠ S181), G214 (= G182), T216 (≠ M184), G261 (≠ E230), S305 (≠ A274), P331 (≠ N300), D332 (= D301)
binding s-adenosylmethionine: P376 (≠ D345), G396 (≠ D366), F397 (≠ V367), D398 (≠ S368), Q399 (= Q369)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 8, 9, 10, 11, 12, 20, 21, 22, 23
binding s-adenosylmethionine: 476, 478, 479

3pc2A Full length structure of cystathionine beta-synthase from drosophila (see paper)
32% identity, 86% coverage: 13:407/457 of query aligns to 48:453/500 of 3pc2A

query
sites
3pc2A

T

T

P

P

L

L

V

V

Y

K

I

L

G

N

Q

N

M

I

-

P

-

A

A

S

R

D

Y

G

L

I

K

E

A

C

K

E

V

M

M

Y

A

A

K

K

L

C

E

E

Y

F

M

L

N

N

P

P

A

G

C

G

S

S

H

V

Y
x
K

Y

D

R

R

A

I

A

G

S

Y

A

R

V

M

I

V

R

Q

D

D

A

A

E

E

R

Q

K

G

L

L

I

L

H

K

P

P

G

G

M

Y

T

T

L

I

V

I

D

E

W

P

T

T

Y

S

G

G

N
|
N

S

T

G

G

I

I

A

G

L

L

A

A

M

M

A

A

G

C

V

A

S

V

R

K

G

G

Y

Y

K

K

L

C

L

I

A

V

A

M

P

P

D

E

K

K

I

M

S

S

N

N

E

E

K

K

K

V

D

S

V

A

L

L

K

R

A

T

L

L

G

G

A

A

E

K

I

I

V

I

I

R

T

T

P

P

S

T

D

E

A

A

L

A

P

Y

E

D

E

S

P

P

R

E

S

G

C

L

M

I

K

Y

V

V

A

A

E

Q

S

Q

L

L

V

Q

R

R

N

E

I

T

P

P

N

N

A

S

F

I

F

V

A

L

N

D

L

Q

Y

Y

E

R

N

N

P
x
A

V

G

S

N

R
x
P

D
x
L

V

A

H

H

S
x
Y

N

D

S

G

T

T

G

A

R

A

E

E

I

I

L

L

Q

W

Q

Q

T

L

E

D

S

N

R

K

V

V

T

D

H

M

V

I

F

V

V

V

P

S

M
x
A

I
x
G

S
x
T

G
x
A

A

G

M
x
T

I

I

S

S

G

G

I

I

G

G

H
x
R

L

K

L

I

K

K

A

E

E

Q

K

V

P

P

S

S

I

C

R

Q

I

I

I

V

G

G

V

V

E

D

P

P

E

Y

G

G

S

S

I

I

Y

L

-

A

-

R

-

P

Q

A

S

E

L

L

F

N

R

K

K

T

G

D

V

V

Q

Q

D

-

K

-

P

-

A

-

A

F

Y

Y

E

E

L

V

E

E

E
x
G

I

I

G

G

A

Y

L

D

Q

F

P

P

S

P

G

T

F

V

W

F

D

D

P

D

S

T

V

V

I

V

D

D

D

V

I

W

V

T

Q

K

V

I

S

G

D

D

N

S

D

D

A

C

F

F

N

P

C

M

G

S

R

R

E

R

L

L

L

N

R

A

A

E

E

E

A

G

I

L

F

L

A

C

G

G

A
x
S

S

S

G

G

A

G

V

A

M

M

A

H

A

A

L

L

H

E

S

H

A

A

A

R

A

K

Y

L

G

K

E

K

N

G

D

Q

C

R

I

C

V

V

A

V

V

I

L

L

N
x
P

D
|
D

F

G

G

I

G

R

Y

N

Y

Y

L

M

S

T

K

K

M

F

F

V

R

S

D

D

G

N

W

W

M

M

K

E

Q

A

K

R

G

N

Y

F

Y

K

R

E

K

P

A

V

K

N

T

E

A

H

L

G

E

H

Q

W

I

W

T

W

A

S

E

L

D

A

I

I

L

A

Q

E

L

L

K

E

A

L

R

P

K

A

D

P

L

P

I

V

F

I

A

L

N

K

P

S

E

D

A

A

T

T

L

V

A

G

E

E

V

A

F

I

E

A

M

L

M

M

K

K

Q

K

N

H

D

R

V

V

S

D

Q

Q

L

L

P

P

I

V

V

V

S

D

Y

Q

-

D

G

G

T

S

P

V

I

L

G

G

S

V

I

V

S

G

E

Q

N

E

K

T

I

L

L

I

S

T

I

Q

L

I

I

V

E

S

N

M

D

N

-

R

-

Q

-

S

-

D

-

P

-

A

-

I

E

K

A

A

M

L

N

N

S

K

K

R

V

V

V

I

G

R

F

L

M

N

E

E

active site: K80 (≠ Y43), S310 (≠ A274)
binding protoporphyrin ix containing fe: A187 (≠ P150), P190 (≠ R153), L191 (≠ D154), Y194 (≠ S157), R227 (≠ H190)
binding pyridoxal-5'-phosphate: K80 (≠ Y43), N110 (= N73), A216 (≠ M179), G217 (≠ I180), T218 (≠ S181), A219 (≠ G182), T221 (≠ M184), G266 (≠ E230), S310 (≠ A274), P336 (≠ N300), D337 (= D301)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 12, 13, 14, 15, 16, 24, 25, 26

3pc4A Full length structure of cystathionine beta-synthase from drosophila in complex with serine (see paper)
32% identity, 86% coverage: 13:407/457 of query aligns to 50:455/504 of 3pc4A

query
sites
3pc4A

T

T

P

P

L

L

V

V

Y

K

I

L

G

N

Q

N

M

I

-

P

-

A

A

S

R

D

Y

G

L

I

K

E

A

C

K

E

V

M

M

Y

A

A

K

K

L

C

E

E

Y

F

M

L

N

N

P

P

A

G

C

G

S

S

H

V

Y
x
K

Y

D

R

R

A

I

A

G

S

Y

A

R

V

M

I

V

R

Q

D

D

A

A

E

E

R

Q

K

G

L

L

I

L

H

K

P

P

G

G

M

Y

T

T

L

I

V

I

D

E

W

P

T
|
T

Y
x
S

G

G

N
|
N

S
x
T

G

G

I

I

A

G

L

L

A

A

M

M

A

A

G

C

V

A

S

V

R

K

G

G

Y

Y

K

K

L

C

L

I

A

V

A

M

P

P

D

E

K

K

I

M

S

S

N

N

E

E

K

K

K

V

D

S

V

A

L

L

K

R

A

T

L

L

G

G

A

A

E

K

I

I

V

I

I

R

T

T

P

P

S

T

D

E

A

A

L

A

P

Y

E

D

E

S

P

P

R

E

S

G

C

L

M

I

K

Y

V

V

A

A

E

Q

S

Q

L

L

V

Q

R

R

N

E

I

T

P

P

N

N

A

S

F

I

F

V

A

L

N

D

L
x
Q

Y

Y

E

R

N

N

P
x
A

V

G

S

N

R
x
P

D
x
L

V

A

H

H

S
x
Y

N

D

S

G

T

T

G

A

R

A

E

E

I

I

L

L

Q

W

Q

Q

T

L

E

D

S

N

R

K

V

V

T

D

H

M

V

I

F

V

V

V

P

S

M
x
A

I
x
G

S
x
T

G
x
A

A

G

M
x
T

I

I

S

S

G

G

I

I

G

G

H
x
R

L

K

L

I

K

K

A

E

E

Q

K

V

P

P

S

S

I

C

R

Q

I

I

I

V

G

G

V

V

E

D

P

P

E

Y

G

G

S

S

I

I

Y

L

-

A

-

R

-

P

Q

A

S

E

L

L

F

N

R

K

K

T

G

D

V

V

Q

Q

D

-

K

-

P

-

A

-

A

F

Y

Y

E

E

L

V

E

E

E
x
G

I
|
I

G

G

A
x
Y

L

D

Q

F

P

P

S

P

G
x
T

F

V

W

F

D

D

P

D

S

T

V

V

I

V

D

D

D

V

I

W

V

T

Q

K

V

I

S

G

D

D

N

S

D

D

A

C

F

F

N

P

C

M

G

S

R

R

E

R

L

L

L

N

R

A

A

E

E

E

A

G

I

L

F

L

A

C

G

G

A
x
S

S

S

G

G

A

G

V

A

M

M

A

H

A

A

L

L

H

E

S

H

A

A

A

R

A

K

Y

L

G

K

E

K

N

G

D

Q

C

R

I

C

V

V

A

V

V

I

L

L

N
x
P

D
|
D

F

G

G

I

G

R

Y

N

Y

Y

L

M

S

T

K

K

M

F

F

V

R

S

D

D

G

N

W

W

M

M

K

E

Q

A

K

R

G

N

Y

F

Y

K

R

E

K

P

A

V

K

N

T

E

A

H

L

G

E

H

Q

W

I

W

T

W

A

S

E

L

D

A

I

I

L

A

Q

E

L

L

K

E

A

L

R

P

K

A

D

P

L

P

I

V

F

I

A

L

N

K

P

S

E

D

A

A

T

T

L

V

A

G

E

E

V

A

F

I

E

A

M

L

M

M

K

K

Q

K

N

H

D

R

V

V

S

D

Q

Q

L

L

P

P

I

V

V

V

S

D

Y

Q

-

D

G

G

T

S

P

V

I

L

G

G

S

V

I

V

S

G

E

Q

N

E

K

T

I

L

L

I

S

T

I

Q

L

I

I

V

E

S

N

M

D

N

-

R

-

Q

-

S

-

D

-

P

-

A

-

I

E

K

A

A

M

L

N

N

S

K

K

R

V

V

V

I

G

R

F

L

M

N

E

E

active site: K82 (≠ Y43), S312 (≠ A274)
binding protoporphyrin ix containing fe: A189 (≠ P150), P192 (≠ R153), L193 (≠ D154), Y196 (≠ S157), R229 (≠ H190), T276 (≠ G238)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: K82 (≠ Y43), T109 (= T70), S110 (≠ Y71), N112 (= N73), T113 (≠ S74), Q185 (≠ L146), A218 (≠ M179), G219 (≠ I180), T220 (≠ S181), A221 (≠ G182), T223 (≠ M184), G268 (≠ E230), I269 (= I231), Y271 (≠ A233), S312 (≠ A274), P338 (≠ N300), D339 (= D301)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28

3pc3A Full length structure of cystathionine beta-synthase from drosophila in complex with aminoacrylate (see paper)
32% identity, 86% coverage: 13:407/457 of query aligns to 50:455/504 of 3pc3A

query
sites
3pc3A

T

T

P

P

L

L

V

V

Y

K

I

L

G

N

Q

N

M

I

-

P

-

A

A

S

R

D

Y

G

L

I

K

E

A

C

K

E

V

M

M

Y

A

A

K

K

L

C

E

E

Y

F

M

L

N

N

P

P

A

G

C

G

S

S

H

V

Y
x
K

Y

D

R

R

A

I

A

G

S

Y

A

R

V

M

I

V

R

Q

D

D

A

A

E

E

R

Q

K

G

L

L

I

L

H

K

P

P

G

G

M

Y

T

T

L

I

V

I

D

E

W

P

T
|
T

Y
x
S

G

G

N
|
N

S
x
T

G

G

I

I

A

G

L

L

A

A

M

M

A

A

G

C

V

A

S

V

R

K

G

G

Y

Y

K

K

L

C

L

I

A

V

A

M

P

P

D

E

K

K

I

M

S

S

N

N

E

E

K

K

K

V

D

S

V

A

L

L

K

R

A

T

L

L

G

G

A

A

E

K

I

I

V

I

I

R

T

T

P

P

S

T

D

E

A

A

L

A

P

Y

E

D

E

S

P

P

R

E

S

G

C

L

M

I

K

Y

V

V

A

A

E

Q

S

Q

L

L

V

Q

R

R

N

E

I

T

P

P

N

N

A

S

F

I

F

V

A

L

N

D

L
x
Q

Y

Y

E

R

N

N

P
x
A

V

G

S

N

R
x
P

D
x
L

V

A

H

H

S
x
Y

N

D

S

G

T

T

G

A

R

A

E

E

I

I

L

L

Q

W

Q

Q

T

L

E

D

S

N

R

K

V

V

T

D

H

M

V

I

F

V

V

V

P

S

M
x
A

I
x
G

S
x
T

G
x
A

A

G

M
x
T

I

I

S

S

G

G

I

I

G

G

H
x
R

L

K

L

I

K

K

A

E

E

Q

K

V

P

P

S

S

I

C

R

Q

I

I

I

V

G

G

V

V

E

D

P

P

E

Y

G

G

S

S

I

I

Y

L

-

A

-

R

-

P

Q

A

S

E

L

L

F

N

R

K

K

T

G

D

V

V

Q

Q

D

-

K

-

P

-

A

-

A

F

Y

Y

E

E

L

V

E

E

E
x
G

I
|
I

G

G

A

Y

L

D

Q

F

P

P

S

P

G

T

F

V

W

F

D

D

P

D

S

T

V

V

I

V

D

D

D

V

I

W

V

T

Q

K

V

I

S

G

D

D

N

S

D

D

A

C

F

F

N

P

C

M

G

S

R

R

E

R

L

L

L

N

R

A

A

E

E

E

A

G

I

L

F

L

A

C

G

G

A
x
S

S

S

G

G

A

G

V

A

M

M

A

H

A

A

L

L

H

E

S

H

A

A

A

R

A

K

Y

L

G

K

E

K

N

G

D

Q

C

R

I

C

V

V

A

V

V

I

L

L

N
x
P

D
|
D

F

G

G

I

G

R

Y

N

Y

Y

L

M

S

T

K

K

M

F

F

V

R

S

D

D

G

N

W

W

M

M

K

E

Q

A

K

R

G

N

Y

F

Y

K

R

E

K

P

A

V

K

N

T

E

A

H

L

G

E

H

Q

W

I

W

T

W

A

S

E

L

D

A

I

I

L

A

Q

E

L

L

K

E

A

L

R

P

K

A

D

P

L

P

I

V

F

I

A

L

N

K

P

S

E

D

A

A

T

T

L

V

A

G

E

E

V

A

F

I

E

A

M

L

M

M

K

K

Q

K

N

H

D

R

V

V

S

D

Q

Q

L

L

P

P

I

V

V

V

S

D

Y

Q

-

D

G

G

T

S

P

V

I

L

G

G

S

V

I

V

S

G

E

Q

N

E

K

T

I

L

L

I

S

T

I

Q

L

I

I

V

E

S

N

M

D

N

-

R

-

Q

-

S

-

D

-

P

-

A

-

I

E

K

A

A

M

L

N

N

S

K

K

R

V

V

V

I

G

R

F

L

M

N

E

E

active site: K82 (≠ Y43), S312 (≠ A274)
binding protoporphyrin ix containing fe: A189 (≠ P150), P192 (≠ R153), L193 (≠ D154), Y196 (≠ S157), R229 (≠ H190)
binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K82 (≠ Y43), T109 (= T70), S110 (≠ Y71), N112 (= N73), T113 (≠ S74), Q185 (≠ L146), A218 (≠ M179), G219 (≠ I180), T220 (≠ S181), A221 (≠ G182), T223 (≠ M184), G268 (≠ E230), I269 (= I231), S312 (≠ A274), P338 (≠ N300), D339 (= D301)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28

1jbqA Structure of human cystathionine beta-synthase: a unique pyridoxal 5'- phosphate dependent hemeprotein (see paper)
36% identity, 69% coverage: 5:321/457 of query aligns to 37:347/348 of 1jbqA

query
sites
1jbqA

D

D

I

I

F

L

G

K

I

K

S

I

T

G

E

D

T

T

P

P

L

M

V

V

Y

R

I

I

G

N

Q

K

M

I

A

G

R

K

Y

K

-

F

-

G

L

L

K

K

A

C

K

E

V

L

M

L

A

A

K

K

L

C

E

E

Y

F

M

F

N

N

P

A

A

G

C

G

S

S

H

V

Y
x
K

Y

D

R

R

A

I

A

S

S

L

A

R

V

M

I

I

R

E

D

D

A

A

E

E

E

R

R

D

K

G

L

T

I

L

H

K

P

P

G

G

M

D

T

T

L

I

V

I

D

E

W

P

T

T

Y
x
S

G

G

N
|
N

S

T

G

G

I

I

A

G

L

L

A

A

M

L

A

A

G

A

V

A

S

V

R

R

G

G

Y

Y

K

R

L

C

L

I

A

V

A

M

P

P

D

E

K

K

I

M

S

S

N

S

E

E

K

K

K

V

D

D

V

V

L

L

K

R

A

A

L

L

G

G

A

A

E

E

I

I

V

V

I

R

T

T

P

P

S

T

D

E

A

-

L

-

P

-

E

-

P

-

R

-

S

S

C

H

M

V

K

G

V

V

A

A

E

W

S

R

L

L

V

K

R

N

N

E

I

I

P

P

N

N

A

S

F

H

F

I

A

L

N

D

L

Q

Y

Y

E

R

N

N

P
x
A

V

S

S

N

R
x
P

D
x
L

V

A

H

H

S
x
Y

N

D

S

T

T

T

G

A

R

D

E

E

I

I

L

L

Q

Q

T

C

E

D

S

G

R

K

V

L

T

D

H

M

V

L

F

V

V

A

P

S

M
x
V

I
x
G

S
x
T

G
|
G

A
x
G

M
x
T

I

I

S

T

G

G

I

I

G

A

H
x
R

L

K

L

L

K

K

A

E

E

K

K

C

P

P

S

G

I

C

R

R

I

I

G

G

V

V

E
x
D

P

P

E

E

G

G

S
|
S

I

I

Y
x
L

Q

A

S

E

L

P

F

E

R

E

K

L

G

N

V

-

Q

Q

D

T

K

E

P

Q

A

T

Y

Y

E

E

L

V

E

E

E
x
G

I

I

G

G

A

Y

L

D

Q

F

P

I

S

P

G

T

F

V

W

L

D

D

P

R

S

T

V

V

I

V

D

D

D

K

I

W

V

F

Q

K

V

S

S

N

D

D

N

E

D

E

A

A

F

F

N

T

C

F

G

A

R

R

E

M

L

L

L

I

R

A

A

Q

E

E

A

G

I

L

F

L

A

C

G

G

A
x
S

S

A

G

G

A

S

V

T

M

V

A

A

A

V

A

A

L

V

H

K

S

A

A

A

A

Q

A

E

Y

L

G

Q

E

E

N

G

D

Q

C

R

I

C

V

V

A

V

V

I

L

L

N
x
P

D
|
D

F

S

G

V

G

R

Y

N

Y

Y

L

M

S

T

K

K

M

F

F

L

R

S

D

D

G

R

W

W

M

M

K

L

Q

Q

K

K

G

G

Y

F

active site: K77 (≠ Y43), S105 (≠ Y71), D232 (≠ E205), S236 (= S209), L238 (≠ Y211), S300 (≠ A274), P326 (≠ N300)
binding protoporphyrin ix containing fe: A177 (≠ P150), P180 (≠ R153), L181 (≠ D154), Y184 (≠ S157), R217 (≠ H190)
binding pyridoxal-5'-phosphate: K77 (≠ Y43), N107 (= N73), V206 (≠ M179), G207 (≠ I180), T208 (≠ S181), G209 (= G182), G210 (≠ A183), T211 (≠ M184), G256 (≠ E230), S300 (≠ A274), P326 (≠ N300), D327 (= D301)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 9, 10, 11, 12, 16, 20, 21, 22, 23

Q2V0C9 Cystathionine beta-synthase; EC 4.2.1.22 from Apis mellifera (Honeybee) (see paper)
35% identity, 85% coverage: 5:392/457 of query aligns to 38:428/504 of Q2V0C9

query
sites
Q2V0C9

D

D

I

I

F

L

G

T

I

A

S

I

T

G

E

Q

T

T

P

P

L

L

V

I

Y

K

I

L

G

N

Q

N

M

I

A

P

R

K

Y

S

-

Y

-

G

L

I

K

K

A

C

K

E

V

I

M

Y

A

A

K

K

L

C

E

E

Y

F

M

L

N

N

P

P

A

G

C

G

S

S

H

V

Y
x
K

Y

D

R

R

A

I

A

A

S

Y

A

R

V

M

I

I

R

Q

D

D

A

A

E

E

E

D

R

K

K

G

L

L

I

L

H

K

P

P

G

G

M

C

T

T

L

I

V

I

D

E

W

P

T

T

Y

S

G

G

N
|
N

S

T

G

G

I

I

A

G

L

L

A

A

M

M

A

A

G

A

V

A

S

V

R

R

G

G

Y

Y

K

K

L

C

L

I

A

V

A

M

P

P

D

E

K

K

I

M

S

S

N

D

E

E

K

K

K

I

D

S

V

T

L

L

K

Y

A

A

L

L

G

G

A

A

E

K

I

I

V

I

I

R

T

T

P

P

S

T

D

E

A

A

L

S

P

W

E

H

E

S

P

P

R

E

S

A

C

H

M

I

K

S

V

V

A

A

E

Q

S

K

L

L

V

Q

R

K

N

E

I

I

P

P

N

N

A

S

F

I

A

L

N

D

L

Q

Y

Y

E

T

N

N

P

P

V

G

S

N

R

P

D

L

V

A

H

H

S

Y

N

D

S

Q

T

T

G

A

R

I

E

E

I

I

L

W

Q

K

Q

Q

T

C

E

E

S

G

R

K

V

I

T

D

H

Y

V

L

F

V

V

A

P

G

M

A

I
x
G

S
x
T

G
|
G

A
x
G

M
x
T

I

I

S

S

G

G

I

I

G

G

H

R

L

K

L

L

K

K

A

E

E

L

K

S

P

P

S

N

I

I

R

K

I

I

G

A

V

V

E

D

P

P

E

K

G

G

S

S

I

I

Y

L

Q

D

S

P

L

-

F

-

R

S

K

S

G

D

V

S

Q

Q

D

N

K

E

P

V

A

G

A

F

Y

Y

E

E

L

V

E

E

E

G

I

I

G

G

A

Y

L

D

Q

F

P

I

S

P

G

T

F

V

W

L

D

D

P

R

S

N

V

V

I

I

D

D

D

K

I

W

V

I

Q

K

V

T

S

E

D

D

N

N

D

E

A

S

F

L

N

N

C

A

G

A

R

R

E

M

L

L

L

I

R

R

A

Q

E

E

A

G

I

L

F

L

A

C

G

G

A
x
S

S

S

G

G

A

A

V

A

M

L

A

I

A

A

L

L

H

K

S

I

A

A

A

K

A

D

Y

I

G

P

E

E

N

E

D

K

C

R

I

M

V

V

A

I

V

I

L

L

N

P

D

D

F

G

G

I

G

R

Y

N

Y

Y

L

L

S

T

K

K

M

F

F

V

R

S

D

E

G

Y

W

W

M

M

K

E

Q

T

K

R

G

G

Y

F

Y

L

R

Q

K

P

-

V

A

C

K

Q

T

N

A

E

L

M

E

N

Q

K

I

W

T

W

A

W

E

N

-

M

D

K

I

I

L

S

Q

N

L

L

K

S

A

F

R

D

K

K

D

Q

L

S

I

L

F

L

A

K

N

E

P

N

E

T

A

V

T

T

L

C

A

Q

E

E

V

A

F

M

E

H

M

M

M

L

K

K

Q

N

N

A

D

D

V

S

S

Q

Q

L

L

L

P

V

I

I

V

S

S

D

Y

D

G

N

T

I

P

H

I

I

-

K

G

G

S

V

I

I

S

S

E

L

N

N

K

K

I

L

L

T

S

S

I

Y

L

V

I

I

K78 (≠ Y43) modified: N6-(pyridoxal phosphate)lysine
N108 (= N73) binding pyridoxal 5'-phosphate
GTGGT 215:219 (≠ ISGAM 180:184) binding pyridoxal 5'-phosphate
S307 (≠ A274) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

12 binding axial binding residue
23 binding axial binding residue

5ohxA Structure of active cystathionine b-synthase from apis mellifera (see paper)
35% identity, 85% coverage: 5:392/457 of query aligns to 34:421/488 of 5ohxA

query
sites
5ohxA

D

D

I

I

F

L

G

T

I

A

S

I

T

G

E

Q

T

T

P

P

L

L

V

I

Y

K

I

L

G

N

Q

N

M

I

A

P

R

K

Y

S

-

Y

-

G

L

I

K

K

A

C

K

E

V

I

M

Y

A

A

K

K

L

C

E

E

Y

F

M

L

N

N

P

P

A

G

C

G

S

S

H

V

Y
x
K

Y

D

R

R

A

I

A

A

S

Y

A

R

V

M

I

I

R

Q

D

D

A

A

E

E

E

D

R

K

K

G

L

L

I

L

H

K

P

P

G

G

M

C

T

T

L

I

V

I

D

E

W

P

T

T

Y

S

G

G

N
|
N

S

T

G

G

I

I

A

G

L

L

A

A

M

M

A

A

G

A

V

A

S

V

R

R

G

G

Y

Y

K

K

L

C

L

I

A

V

A

M

P

P

D

E

K

K

I

M

S

S

N

D

E

E

K

K

K

I

D

S

V

T

L

L

K

Y

A

A

L

L

G

G

A

A

E

K

I

I

V

I

I

R

T

T

P

P

S

T

D

E

A

A

L

S

P

W

E

H

E

S

P

P

R

E

S

A

C

H

M

I

K

S

V

V

A

A

E

Q

S

K

L

L

V

Q

R

K

N

E

I

I

P

P

N

N

A

S

F

I

A

L

N

D

L

Q

Y

Y

E

T

N

N

P
|
P

V

G

S

N

R
x
P

D

L

V

A

H

H

S
x
Y

N

D

S

Q

T

T

G

A

R

I

E

E

I

I

L

W

Q

K

Q

Q

T

C

E

E

S

G

R

K

V

I

T

D

H

Y

V

L

F

V

V

A

P
x
G

M

A

I
x
G

S
x
T

G
|
G

A
x
G

M
x
T

I

I

S

S

G

G

I

I

G

G

H
x
R

L

K

L

L

K

K

A

E

E

L

K

S

P

P

S

N

I

I

R

K

I

I

G

A

V

V

E

D

P

P

E

K

G

G

S

S

I

I

Y

L

Q

D

S

P

L

-

F

-

R

-

K

-

G

-

V

S

Q

S

D

D

K

E

P

V

A

G

A

F

Y

Y

E

E

L

V

E

E

E
x
G

I

I

G

G

A

Y

L

D

Q

F

P

I

S

P

G

T

F

V

W

L

D

D

P

R

S

N

V

V

I

I

D

D

D

K

I

W

V

I

Q

K

V

T

S

E

D

D

N

N

D

E

A

S

F

L

N

N

C

A

G

A

R

R

E

M

L

L

L

I

R

R

A

Q

E

E

A

G

I

L

F

L

A

C

G

G

A
x
S

S

S

G

G

A

A

V

A

M

L

A

I

A

A

L

L

H

K

S

I

A

A

A

K

A

D

Y

I

G

P

E

E

N

E

D

K

C

R

I

M

V

V

A

I

V

I

L

L

N
x
P

D
|
D

F

G

G

I

G

R

Y

N

Y

Y

L

L

S

T

K

K

M

F

F

V

R

S

D

E

G

Y

W

W

M

M

K

E

Q

T

K

R

G

G

Y

F

Y

L

R

Q

K

P

-

V

A

C

K

Q

T

N

A

E

L

M

E

N

Q

K

I

W

T

W

A

W

E

N

-

M

D

K

I

I

L

S

Q

N

L

L

K

S

A

F

R

D

K

K

D

Q

L

S

I

L

F

L

A

K

N

E

P

N

E

T

A

V

T

T

L

C

A

Q

E

E

V

A

F

M

E

H

M

M

M

L

K

K

Q

N

N

A

D

D

V

S

S

Q

Q

L

L

L

P

V

I

I

V

S

S

D

Y

D

G

N

T

I

P

H

I

I

-

K

G

G

S

V

I

I

S

S

E

L

N

N

K

K

I

L

L

T

S

S

I

Y

L

V

I

I

binding protoporphyrin ix containing fe: P181 (= P150), P184 (≠ R153), Y188 (≠ S157), R221 (≠ H190)
binding pyridoxal-5'-phosphate: K74 (≠ Y43), N104 (= N73), G209 (≠ P178), G211 (≠ I180), T212 (≠ S181), G213 (= G182), G214 (≠ A183), T215 (≠ M184), G256 (≠ E230), S300 (≠ A274), P326 (≠ N300), D327 (= D301)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 4, 7, 8, 9, 10, 14, 16, 17, 18, 19

6xylA Crystal structure of delta466-491 cystathionine beta-synthase from toxoplasma gondii with l-serine (see paper)
32% identity, 85% coverage: 13:402/457 of query aligns to 19:414/468 of 6xylA

query
sites
6xylA

T

T

P

P

L

M

V

V

Y

R

I

M

G

K

Q

R

M

L

A

A

R

K

Y

V

-

Y

-

G

L

L

K

E

A

C

K

D

V

L

M

L

A

A

K

K

L

C

E

E

Y

F

M

M

N

S

P

A

A

G

C

G

S

S

H

V

Y
x
K

Y

D

R

R

A

I

A

G

S

K

A

A

V

M

I

V

R

E

D

K

A

A

E

E

E

R

R

E

K

G

L

R

I

L

H

K

P

A

G

G

M

D

T

T

L

L

V

I

D

E

W

P

T

T

Y

S

G

G

N

N

S
x
T

G

G

I

I

A

G

L

L

A

A

M

L

A

A

G

A

V

A

S

V

R

R

G

G

Y

Y

K

R

L

M

L

I

L

V

A

T

A

M

P

P

D

A

K

K

I

M

S

S

N

A

E

E

K

K

K

S

D

N

V

I

L

M

K

K

A

C

L

L

G

G

A

A

E

E

I

I

V

V

I

R

T

T

P

P

S

T

D

E

A

A

L

A

P

W

E

N

E

D

P

E

R

N

S

S

C

H

M

M

K

G

V

V

A

A

E

A

S

K

L

L

V

Q

R

R

N

E

I

L

P

E

N

N

A

A

F

H

F

I

A

L

N

D

L
x
Q

Y

Y

E

N

N

N

P

T

V

A

S

N

R

P

D

M

V

V

H

H

S

Y

N

D

S

V

T

T

G

A

R

E

E

E

I

I

L

I

Q

T

Q

Q

T

C

E

D

S

G

R

D

V

I

T

D

H

M

V

V

F

V

V

I

P

G

M

A

I
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

T

G

G

I

I

G

G

H

R

L

K

L

I

K

K

A

E

E

R

K

C

P

P

S

K

I

C

R

K

I

V

G

G

V

V

E

D

P

P

E

K

G

G

S

S

I

I

Y

L

Q

A

S

V

L

P

F

D

R

S

K

L

G

N

V

D

Q

E

D

K

K

R

P

L

A

Q

A

S

Y

Y

E

E

L

V

E

E

E
x
G

I
|
I

G

G

A
x
Y

L

D

Q

F

P

V

S

P

G

G

F

V

W

L

D

D

P

R

S

K

V

V

I

V

D

D

D

E

I

W

V

V

Q

K

V

V

S

G

D

D

N

A

D

E

A

S

F

F

N

T

C

T

G

A

R

R

E

A

L

I

R

R

A

N

E

E

A

G

I

L

F

F

A

V

G

G

A
x
S

S

S

G

G

A

A

V

N

M

V

A

W

A

G

A

A

L

L

H

Q

S

A

A

A

A

R

A

Q

Y

L

G

K

E

K

N

G

D

Q

C

K

I

C

V

V

A

V

V

L

L

L

N
x
P

D
|
D

F

S

G

S

G

R

Y

N

Y

Y

L

M

S

S

K

K

M

F

F

I

R

S

D

D

G

E

W

W

M

M

K

A

Q

E

K

H

G

G

Y

F

Y

A

-

P

-

E

-

D

-

G

R

A

K

K

A

V

K

K

T

E

A

R

L

E

E

K

Q

Q

I

F

T

G

A

G

E

A

D

R

I

I

L

R

Q

D

L

L

K

L

A

S

R

E

K

T

D

G

L

A

I

T

-

S

F

F

A

V

N

T

P

A

E

R

A

L

T

S

L

V

A

E

E

D

V

V

F

I

E

K

M

M

K

H

Q

E

N

T

D

K

V

V

S

K

Q

E

L

V

P

-

I

I

V

V

S

T

Y

E

G

D

T

S

P

L

I

V

G

G

S

V

I

L

S

S

E

E

N

D

K

H

I

I

L

A

S

H

I

S

L

L

I

Q

E

S

N

G

D

R

E

C

A

A

M

M

N

Q

S

S

K

P

V

V

binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K51 (≠ Y43), T82 (≠ S74), Q154 (≠ L146), G188 (≠ I180), T189 (≠ S181), G190 (= G182), T192 (≠ M184), G238 (≠ E230), I239 (= I231), Y241 (≠ A233), S282 (≠ A274), P308 (≠ N300), D309 (= D301)

6xwlC Cystathionine beta-synthase from toxoplasma gondii (see paper)
32% identity, 85% coverage: 13:402/457 of query aligns to 19:422/477 of 6xwlC

query
sites
6xwlC

T

T

P

P

L

M

V

V

Y

R

I

M

G

K

Q

R

M

L

A

A

R

K

Y

V

-

Y

-

G

L

L

K

E

A

C

K

D

V

L

M

L

A

A

K

K

L

C

E

E

Y

F

M

M

N

S

P

A

A

G

C

G

S

S

H

V

Y
x
K

Y

D

R

R

A

I

A

G

S

K

A

A

V

M

I

V

R

E

D

K

A

A

E

E

E

R

R

E

K

G

L

R

I

L

H

K

P

A

G

G

M

D

T

T

L

L

V

I

D

E

W

P

T

T

Y

S

G

G

N
|
N

S

T

G

G

I

I

A

G

L

L

A

A

M

L

A

A

G

A

V

A

S

V

R

R

G

G

Y

Y

K

R

L

M

L

I

L

V

A

T

A

M

P

P

D

A

K

K

I

M

S

S

N

A

E

E

K

K

K

S

D

N

V

I

L

M

K

K

A

C

L

L

G

G

A

A

E

E

I

I

V

V

I

R

T

T

P

P

S

T

D

E

A

A

L

A

P

W

E

N

E

D

P

E

R

N

S

S

C

H

M

M

K

G

V

V

A

A

E

A

S

K

L

L

V

Q

R

R

N

E

I

L

P

E

N

N

A

A

F

H

F

I

A

L

N

D

L

Q

Y

Y

E

N

N

N

P

T

V

A

S

N

R

P

D

M

V

V

H

H

S

Y

N

D

S

V

T

T

G

A

R

E

E

E

I

I

L

I

Q

T

Q

Q

T

C

E

D

S

G

R

D

V

I

T

D

H

M

V

V

F

V

V

I

P

G

M

A

I
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

T

G

G

I

I

G

G

H

R

L

K

L

I

K

K

A

E

E

R

K

C

P

P

S

K

I

C

R

K

I

V

G

G

V

V

E

D

P

P

E

K

G

G

S

S

I

I

Y

L

Q

A

S

V

L

P

F

D

R

S

K

L

G

N

V

D

Q

E

D

K

K

R

P

L

A

Q

A

S

Y

Y

E

E

L

V

E

E

E
x
G

I

I

G

G

A

Y

L

D

Q

F

P

V

S

P

G

G

F

V

W

L

D

D

P

R

S

K

V

V

I

V

D

D

D

E

I

W

V

V

Q

K

V

V

S

G

D

D

N

A

D

E

A

S

F

F

N

T

C

T

G

A

R

R

E

A

L

I

R

R

A

N

E

E

A

G

I

L

F

F

A

V

G

G

A
x
S

S

S

G

G

A

A

V

N

M

V

A

W

A

G

A

A

L

L

H

Q

S

A

A

A

A

R

A

Q

Y

L

G

K

E

K

N

G

D

Q

C

K

I

C

V

V

A

V

V

L

L

L

N
x
P

D
|
D

F

S

G

S

G

R

Y

N

Y

Y

L

M

S

S

K

K

M

F

F

I

R

S

D

D

G

E

W

W

M

M

K

A

Q

E

K

H

G

G

Y

F

Y

A

-

P

-

E

-

D

-

G

R

A

K

K

A

V

K

K

T

E

A

R

L

E

E

K

Q

Q

I

F

T

G

A

G

E

A

D

R

I

I

L

R

Q

D

L

L

-

L

-

S

-

E

-

T

K

G

A

A

R

T

K

S

D

D

L

V

I

P

F

F

A

V

N

T

P

A

E

R

A

L

T

S

L

V

A

E

E

D

V

V

F

I

E

K

M

M

K

H

Q

E

N

T

D

K

V

V

S

K

Q

E

L

V

P

I

I

V

V

T

-

E

-

D

S

S

Y

E

G

G

T

K

P

T

-

K

-

L

I

V

G

G

S

V

I

L

S

S

E

E

N

D

K

H

I

I

L

A

S

H

I

S

L

L

I

Q

E

S

N

G

D

R

E

C

A

A

M

M

N

Q

S

S

K

P

V

V

binding pyridoxal-5'-phosphate: K51 (≠ Y43), N81 (= N73), G188 (≠ I180), T189 (≠ S181), G190 (= G182), T192 (≠ M184), G238 (≠ E230), S282 (≠ A274), P308 (≠ N300), D309 (= D301)

6c2zA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the plp-aminoacrylate intermediate (see paper)
34% identity, 70% coverage: 2:322/457 of query aligns to 7:337/345 of 6c2zA

query
sites
6c2zA

S

S

N

R

H

H

D

N

I

V

F

I

G

D

I

L

S

V

T

G

E

N

T

T

P

P

L

L

V

I

Y

A

I

L

G

K

Q

K

M

L

A

P

R

K

Y

A

L

L

-

G

-

I

K

K

A

P

K

Q

V

I

M

Y

A

A

K

K

L

L

E

E

Y

L

M

Y

N

N

P

P

A

G

C

G

S

S

H

I

Y
x
K

Y

D

R

R

A

I

A

A

S

K

A

S

V

M

I

V

R

E

D

E

A

A

E

E

E

A

R

S

K

G

L

R

I

I

H

H

P

P

G

S

M

R

-

S

T

T

L

L

V

I

D

E

W

P

T
|
T

Y
x
S

G

G

N
|
N

S
x
T

G

G

I

I

A

G

L

L

A

A

M

L

A

I

G

G

V

A

S

I

R

K

G

G

Y

Y

K

R

L

T

L

I

A

T

A

L

P

P

D

E

K

K

I

M

S

S

N

N

E

E

K

K

K

V

D

S

V

V

L

L

K

K

A

A

L

L

G

G

A

A

E

E

I

I

V

I

I

R

T

T

P

P

S

T

D

A

A

A

L

A

P

W

E

D

E

S

P

P

R

E

S

S

C

H

M

I

K

G

V

V

A

A

E

K

S

K

L

L

V

E

R

K

N

E

I

I

P

P

N

G

A

A

F

V

F

I

A

L

N

D

L
x
Q

Y

Y

E

N

N

N

P

M

V

M

S

N

R

P

D

E

V

A

H

H

S

Y

N

F

S

G

T

T

G

G

R

R

E

E

I

I

L

Q

Q

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

S
x
D

R
x
N

V

L

T

R

H

A

V

V

F

V

V

A

P

G

M
x
A

I
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

S

G

G

I

I

G

S

H

K

L

Y

L

L

K

K

A

E

E

Q

K

N

P

D

S

K

I

I

R

Q

I

I

I

V

G

G

V

A

E

D

P

P

E

F

G

G

S

S

I

I

Y

L

-

A

-

Q

-

P

Q

E

S

N

L

L

F

N

R

K

K

T

G

D

V

I

Q

T

D

D

K

-

P

-

A

-

Y

Y

E

K

L

V

E

E

E
x
G

I

I

G

G

A

Y

L

D

Q

F

P

V

S

P

G

Q

F

V

W

L

D

D

P

R

S

K

V

L

I

I

D

D

D

V

I

W

V

Y

Q

K

V

T

S

D

D

D

N

K

D

P

A

S

F

F

N

K

C

Y

G

A

R

R

E

Q

L

L

L

I

R

S

A

N

E

E

A

G

I

V

F

L

A

V

G

G

A
x
S

S

S

G

G

A

S

V

A

M

F

A

T

A

A

A

V

L

V

H

K

S

Y

A

C

A

E

A

D

Y

H

-

P

-

E

-

L

G

T

E

E

N

D

D

D

C

V

I

I

V

V

A

A

V

I

L

F

N
x
P

D
|
D

F

S

G

I

G

R

Y

S

Y

Y

L

L

S

T

K

K

M

F

F

V

R

D

D

D

G

E

W

W

M

L

K

K

Q

K

K

N

G

N

Y

L

Y

W

binding calcium ion: N180 (vs. gap), D183 (≠ S170), N184 (≠ R171)
binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K50 (≠ Y43), T78 (= T70), S79 (≠ Y71), N81 (= N73), T82 (≠ S74), Q154 (≠ L146), A192 (≠ M179), G193 (≠ I180), T194 (≠ S181), G195 (= G182), T197 (≠ M184), G242 (≠ E230), S286 (≠ A274), P315 (≠ N300), D316 (= D301)

6c2qA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the plp-l-serine intermediate (see paper)
34% identity, 70% coverage: 2:322/457 of query aligns to 7:337/345 of 6c2qA

query
sites
6c2qA

S

S

N

R

H

H

D

N

I

V

F

I

G

D

I

L

S

V

T

G

E

N

T

T

P

P

L

L

V

I

Y

A

I

L

G

K

Q

K

M

L

A

P

R

K

Y

A

L

L

-

G

-

I

K

K

A

P

K

Q

V

I

M

Y

A

A

K

K

L

L

E

E

Y

L

M

Y

N

N

P

P

A

G

C

G

S

S

H

I

Y
x
K

Y

D

R

R

A

I

A

A

S

K

A

S

V

M

I

V

R

E

D

E

A

A

E

E

E

A

R

S

K

G

L

R

I

I

H

H

P

P

G

S

M

R

-

S

T

T

L

L

V

I

D

E

W

P

T
|
T

Y
x
S

G

G

N
|
N

S
x
T

G

G

I

I

A

G

L

L

A

A

M

L

A

I

G

G

V

A

S

I

R

K

G

G

Y

Y

K

R

L

T

L

I

A

T

A

L

P

P

D

E

K

K

I

M

S

S

N

N

E

E

K

K

K

V

D

S

V

V

L

L

K

K

A

A

L

L

G

G

A

A

E

E

I

I

V

I

I

R

T

T

P

P

S

T

D

A

A

A

L

A

P

W

E

D

E

S

P

P

R

E

S

S

C

H

M

I

K

G

V

V

A

A

E

K

S

K

L

L

V

E

R

K

N

E

I

I

P

P

N

G

A

A

F

V

F

I

A

L

N

D

L
x
Q

Y

Y

E

N

N

N

P

M

V

M

S

N

R

P

D

E

V

A

H

H

S

Y

N

F

S

G

T

T

G

G

R

R

E

E

I

I

L

Q

Q

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

S
x
D

R
x
N

V

L

T

R

H

A

V

V

F

V

V

A

P

G

M
x
A

I
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

S

G

G

I

I

G

S

H

K

L

Y

L

L

K

K

A

E

E

Q

K

N

P

D

S

K

I

I

R

Q

I

I

I

V

G

G

V

A

E

D

P

P

E

F

G

G

S

S

I

I

Y

L

-

A

-

Q

-

P

Q

E

S

N

L

L

F

N

R

K

K

T

G

D

V

I

Q

T

D

D

K

-

P

-

A

-

Y

Y

E

K

L

V

E

E

E
x
G

I

I

G

G

A
x
Y

L

D

Q

F

P

V

S

P

G

Q

F

V

W

L

D

D

P

R

S

K

V

L

I

I

D

D

D

V

I

W

V

Y

Q

K

V

T

S

D

D

D

N

K

D

P

A

S

F

F

N

K

C

Y

G

A

R

R

E

Q

L

L

L

I

R

S

A

N

E

E

A

G

I

V

F

L

A

V

G

G

A
x
S

S

S

G

G

A

S

V

A

M

F

A

T

A

A

A

V

L

V

H

K

S

Y

A

C

A

E

A

D

Y

H

-

P

-

E

-

L

G

T

E

E

N

D

D

D

C

V

I

I

V

V

A

A

V

I

L

F

N
x
P

D
|
D

F

S

G

I

G

R

Y

S

Y

Y

L

L

S

T

K

K

M

F

F

V

R

D

D

D

G

E

W

W

M

L

K

K

Q

K

K

N

G

N

Y

L

Y

W

binding calcium ion: N180 (vs. gap), D183 (≠ S170), N184 (≠ R171)
binding L-Serine, N-[[3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]-4-pyridinyl]methylene]: K50 (≠ Y43), T78 (= T70), S79 (≠ Y71), N81 (= N73), T82 (≠ S74), Q154 (≠ L146), A192 (≠ M179), G193 (≠ I180), T194 (≠ S181), G195 (= G182), T197 (≠ M184), G242 (≠ E230), Y245 (≠ A233), S286 (≠ A274), P315 (≠ N300), D316 (= D301)

6c2hA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the catalytic core (see paper)
34% identity, 70% coverage: 2:322/457 of query aligns to 7:337/345 of 6c2hA

query
sites
6c2hA

S

S

N

R

H

H

D

N

I

V

F

I

G

D

I

L

S

V

T

G

E

N

T

T

P

P

L

L

V

I

Y

A

I

L

G

K

Q

K

M

L

A

P

R

K

Y

A

L

L

-

G

-

I

K

K

A

P

K

Q

V

I

M

Y

A

A

K

K

L

L

E

E

Y

L

M

Y

N

N

P

P

A

G

C

G

S

S

H

I

Y
x
K

Y

D

R

R

A

I

A

A

S

K

A

S

V

M

I

V

R

E

D

E

A

A

E

E

E

A

R

S

K

G

L

R

I

I

H

H

P

P

G

S

M

R

-

S

T

T

L

L

V

I

D

E

W

P

T

T

Y

S

G

G

N
|
N

S

T

G

G

I

I

A

G

L

L

A

A

M

L

A

I

G

G

V

A

S

I

R

K

G

G

Y

Y

K

R

L

T

L

I

A

T

A

L

P

P

D

E

K

K

I

M

S

S

N

N

E

E

K

K

K

V

D

S

V

V

L

L

K

K

A

A

L

L

G

G

A

A

E

E

I

I

V

I

I

R

T

T

P

P

S

T

D

A

A

A

L

A

P

W

E

D

E

S

P

P

R

E

S

S

C

H

M

I

K

G

V

V

A

A

E

K

S

K

L

L

V

E

R

K

N

E

I

I

P

P

N

G

A

A

F

V

F

I

A

L

N

D

L

Q

Y

Y

E

N

N

N

P

M

V

M

S

N

R

P

D

E

V

A

H

H

S

Y

N

F

S

G

T

T

G

G

R

R

E

E

I

I

L

Q

Q

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

S
x
D

R
x
N

V

L

T

R

H

A

V

V

F

V

V

A

P

G

M
x
A

I
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

S

G

G

I

I

G

S

H

K

L

Y

L

L

K

K

A

E

E

Q

K

N

P

D

S

K

I

I

R

Q

I

I

I

V

G

G

V

A

E

D

P

P

E

F

G

G

S

S

I

I

Y

L

-

A

-

Q

-

P

Q

E

S

N

L

L

F

N

R

K

K

T

G

D

V

I

Q

T

D

D

K

-

P

-

A

-

Y

Y

E

K

L

V

E

E

E
x
G

I

I

G

G

A

Y

L

D

Q

F

P

V

S

P

G

Q

F

V

W

L

D

D

P

R

S

K

V

L

I

I

D

D

D

V

I

W

V

Y

Q

K

V

T

S

D

D

D

N

K

D

P

A

S

F

F

N

K

C

Y

G

A

R

R

E

Q

L

L

L

I

R

S

A

N

E

E

A

G

I

V

F

L

A

V

G

G

A
x
S

S

S

G

G

A

S

V

A

M

F

A

T

A

A

A

V

L

V

H

K

S

Y

A

C

A

E

A

D

Y

H

-

P

-

E

-

L

G

T

E

E

N

D

D

D

C

V

I

I

V

V

A

A

V

I

L

F

N
x
P

D
|
D

F

S

G

I

G

R

Y

S

Y

Y

L

L

S

T

K

K

M

F

F

V

R

D

D

D

G

E

W

W

M

L

K

K

Q

K

K

N

G

N

Y

L

Y

W

binding calcium ion: N180 (vs. gap), D183 (≠ S170), N184 (≠ R171)
binding pyridoxal-5'-phosphate: K50 (≠ Y43), N81 (= N73), A192 (≠ M179), G193 (≠ I180), T194 (≠ S181), G195 (= G182), T197 (≠ M184), G242 (≠ E230), S286 (≠ A274), P315 (≠ N300), D316 (= D301)

6c4pA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the pmp complex (see paper)
34% identity, 70% coverage: 2:322/457 of query aligns to 6:336/344 of 6c4pA

query
sites
6c4pA

S

S

N

R

H

H

D

N

I

V

F

I

G

D

I

L

S

V

T

G

E

N

T

T

P

P

L

L

V

I

Y

A

I

L

G

K

Q

K

M

L

A

P

R

K

Y

A

L

L

-

G

-

I

K

K

A

P

K

Q

V

I

M

Y

A

A

K

K

L

L

E

E

Y

L

M

Y

N

N

P

P

A

G

C

G

S

S

H

I

Y
x
K

Y

D

R

R

A

I

A

A

S

K

A

S

V

M

I

V

R

E

D

E

A

A

E

E

E

A

R

S

K

G

L

R

I

I

H

H

P

P

G

S

M

R

-

S

T

T

L

L

V

I

D

E

W

P

T

T

Y

S

G

G

N
|
N

S

T

G

G

I

I

A

G

L

L

A

A

M

L

A

I

G

G

V

A

S

I

R

K

G

G

Y

Y

K

R

L

T

L

I

A

T

A

L

P

P

D

E

K

K

I

M

S

S

N

N

E

E

K

K

K

V

D

S

V

V

L

L

K

K

A

A

L

L

G

G

A

A

E

E

I

I

V

I

I

R

T

T

P

P

S

T

D

A

A

A

L

A

P

W

E

D

E

S

P

P

R

E

S

S

C

H

M

I

K

G

V

V

A

A

E

K

S

K

L

L

V

E

R

K

N

E

I

I

P

P

N

G

A

A

F

V

F

I

A

L

N

D

L

Q

Y

Y

E

N

N

N

P

M

V

M

S

N

R

P

D

E

V

A

H

H

S

Y

N

F

S

G

T

T

G

G

R

R

E

E

I

I

L

Q

Q

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

S
x
D

R
x
N

V

L

T

R

H

A

V

V

F

V

V

A

P

G

M
x
A

I
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

S

G

G

I

I

G

S

H

K

L

Y

L

L

K

K

A

E

E

Q

K

N

P

D

S

K

I

I

R

Q

I

I

I

V

G

G

V

A

E

D

P

P

E

F

G

G

S

S

I

I

Y

L

-

A

-

Q

-

P

Q

E

S

N

L

L

F

N

R

K

K

T

G

D

V

I

Q

T

D

D

K

-

P

-

A

-

Y

Y

E

K

L

V

E

E

E
x
G

I

I

G

G

A

Y

L

D

Q

F

P

V

S

P

G

Q

F

V

W

L

D

D

P

R

S

K

V

L

I

I

D

D

D

V

I

W

V

Y

Q

K

V

T

S

D

D

D

N

K

D

P

A

S

F

F

N

K

C

Y

G

A

R

R

E

Q

L

L

L

I

R

S

A

N

E

E

A

G

I

V

F

L

A

V

G

G

A
x
S

S

S

G

G

A

S

V

A

M

F

A

T

A

A

A

V

L

V

H

K

S

Y

A

C

A

E

A

D

Y

H

-

P

-

E

-

L

G

T

E

E

N

D

D

D

C

V

I

I

V

V

A

A

V

I

L

F

N
x
P

D
|
D

F

S

G

I

G

R

Y

S

Y

Y

L

L

S

T

K

K

M

F

F

V

R

D

D

D

G

E

W

W

M

L

K

K

Q

K

K

N

G

N

Y

L

Y

W

binding calcium ion: N179 (vs. gap), D182 (≠ S170), N183 (≠ R171)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: K49 (≠ Y43), N80 (= N73), A191 (≠ M179), G192 (≠ I180), T193 (≠ S181), G194 (= G182), T196 (≠ M184), G241 (≠ E230), S285 (≠ A274), P314 (≠ N300), D315 (= D301)

6vjuB Crystal structure of cystathionine beta synthase from legionella pneumophila with llp, plp, and homocysteine
34% identity, 69% coverage: 5:321/457 of query aligns to 6:316/317 of 6vjuB

query
sites
6vjuB

D

N

I

I

F

L

G

A

I

T

S

I

T

G

E

H

T

T

P

P

L

V

V

V

Y

K

I

I

G

N

Q

R

M

L

A

G

R

K

Y

D

L

L

K

E

A

C

K

E

V

L

M

Y

A

A

K

K

L

C

E

E

Y

F

M

F

N

N

P

P

A

G

C

G

S

S

H

V

Y
x
K

Y

D

R

R

A

I

A

G

S

Y

A

E

V

M

I

V

R

V

D

K

A

A

E

E

E

K

R

E

K

G

L

R

I

I

H

K

P

P

G

G

M

D

T

T

L

L

V

I

D

E

W

P

T

T

Y

S

G

G

N
|
N

S

T

G

G

I

I

A

G

L

I

A

A

M

L

A

A

G

G

V

A

S

V

R

L

G

G

Y

Y

K

K

L

V

L

I

A

T

A

M

P

P

D

E

K

K

I

M

S

S

N

Q

E

E

K

K

K

Q

D

S

V

V

L

L

K

E

A

R

L

L

G

G

A

A

E

I

I

I

V

Y

I

R

T

T

P

P

S

T

D

E

A

A

L

A

P

Y

E

N

E

D

P

P

R

D

S

S

C

H

M

I

K

S

V

L

A

A

E

K

S

K

L

L

V

Q

R

A

N

E

I

I

P

P

N

N

A

S

F

H

F

I

A

L

N

D

L

Q

Y

Y

E

A

N

N

P

P

V

N

S

N

R

P

D

N

V

A

H

H

S

Y

N

F

S

G

T

T

G

A

R

Q

E

E

I

I

L

I

Q

D

T

F

E

G

S

K

R

D

V

L

T

H

H

M

V

V

F

V

V

A

P

G

M

V

I
x
G

S
x
T

G
|
G

A

G

M
x
T

I

I

S

T

G

G

I

I

G

A

H

K

L

R

L

L

K

K

A

E

E

F

K

N

P

P

S

A

I

I

R

K

I

I

G

G

V

A

E

D

P

P

E

E

G

G

S

S

I

I

Y

L

Q

-

S

-

L

-

F

-

R

-

K

-

G

G

V

G

Q

G

D

T

K

E

P

I

A

K

A

S

Y

Y

E

H

L

V

E

E

E
x
G

I

I

G

G

A

Y

L

D

Q

F

P

F

S

P

G

D

F

V

W

L

D

D

P

N

S

T

V

L

I

I

D

D

D

A

I

Y

V

I

Q

K

V

T

S

N

D

D

N

A

D

D

A

S

F

F

N

R

C

T

G

A

R

R

E

R

L

L

L

I

R

K

A

E

E

E

A

G

I

L

F

L

A

I

G

G

A
x
S

S

C

G

G

A

A

V

A

M

M

A

W

A

A

L

L

H

Q

S

A

A

A

A

K

A

S

Y

L

G

S

E

K

N

G

D

Q

C

K

I

C

V

L

A

V

V

I

L

L

N
x
P

D
|
D

F

S

G

I

G

R

Y

N

Y

Y

L

M

S

S

K

K

M

F

F

A

R

N

D

D

G

E

W

W

M

M

K

K

Q

E

K

M

G

G

Y

F

binding pyridoxal-5'-phosphate: K44 (≠ Y43), N74 (= N73), G181 (≠ I180), T182 (≠ S181), G183 (= G182), T185 (≠ M184), G225 (≠ E230), S269 (≠ A274), P295 (≠ N300), D296 (= D301)

Query Sequence

>WP_012467019.1 NCBI__GCF_000020465.1:WP_012467019.1
MSNHDIFGISTETPLVYIGQMARYLKAKVMAKLEYMNPACSHYYRAASAVIRDAEERKLI
HPGMTLVDWTYGNSGIALAMAGVSRGYKLLLAAPDKISNEKKDVLKALGAEIVITPSDAL
PEEPRSCMKVAESLVRNIPNAFFANLYENPVSRDVHSNSTGREILQQTESRVTHVFVPMI
SGAMISGIGHLLKAEKPSIRIIGVEPEGSIYQSLFRKGVQDKPAAYELEEIGALQPSGFW
DPSVIDDIVQVSDNDAFNCGRELLRAEAIFAGGASGAVMAAALHSAAAYGENDCIVAVLN
DFGGYYLSKMFRDGWMKQKGYYRKAKTALEQITAEDILQLKARKDLIFANPEATLAEVFE
MMKQNDVSQLPIVSYGTPIGSISENKILSILIENDEAMNSKVVGFMEPPFPVCQTDATIS
ELSDKLQQSASGILISLSDGRLQLLTKSDLIDALTHK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory