SitesBLAST
Comparing WP_012467298.1 NCBI__GCF_000020465.1:WP_012467298.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3e0iA Cu2+ substituted aquifex aeolicus kdo8ps in complex with pep (see paper)
50% identity, 90% coverage: 20:267/276 of query aligns to 6:256/263 of 3e0iA
- binding copper (ii) ion: C10 (= C24), H184 (= H198), E221 (= E235)
- binding phosphoenolpyruvate: K40 (= K53), K45 (= K58), Q98 (= Q111), P100 (= P113), A101 (= A114), K123 (= K136), R153 (= R166), H184 (= H198)
- binding phosphate ion: R48 (= R61), S49 (= S62), K195 (≠ V209), S196 (= S210)
2a21A Aquifex aeolicus kdo8ps in complex with pep, po4, and zn2+ (see paper)
50% identity, 90% coverage: 20:267/276 of query aligns to 6:256/263 of 2a21A
- binding phosphoenolpyruvate: K40 (= K53), K45 (= K58), Q98 (= Q111), P100 (= P113), A101 (= A114), K123 (= K136), R153 (= R166), H184 (= H198)
- binding phosphate ion: R48 (= R61), S49 (= S62), S196 (= S210)
- binding zinc ion: C10 (= C24), H184 (= H198), E221 (= E235), D232 (= D246)
1jcyA Aquifex aeolicus kdo8p synthase in complex with r5p, pep and cadmium (see paper)
50% identity, 90% coverage: 20:267/276 of query aligns to 6:256/263 of 1jcyA
- binding phosphoenolpyruvate: K40 (= K53), K45 (= K58), Q98 (= Q111), P100 (= P113), A101 (= A114), K123 (= K136), R153 (= R166), H184 (= H198)
- binding ribose-5-phosphate: N47 (= N60), R48 (= R61), S49 (= S62), H184 (= H198), Q187 (= Q201), S196 (= S210)
1fwwA Aquifex aeolicus kdo8p synthase in complex with pep, a5p and cadmium (see paper)
50% identity, 90% coverage: 20:267/276 of query aligns to 6:256/263 of 1fwwA
- binding arabinose-5-phosphate: N47 (= N60), R48 (= R61), S49 (= S62), H184 (= H198), S196 (= S210), D232 (= D246)
- binding phosphoenolpyruvate: K40 (= K53), K45 (= K58), Q98 (= Q111), P100 (= P113), A101 (= A114), K123 (= K136), R153 (= R166), H184 (= H198)
1fwtA Aquifex aeolicus kdo8p synthase in complex with pep, e4p and cadmium (see paper)
50% identity, 90% coverage: 20:267/276 of query aligns to 6:256/263 of 1fwtA
- binding erythose-4-phosphate: N47 (= N60), R48 (= R61), S49 (= S62), S196 (= S210)
- binding phosphoenolpyruvate: K40 (= K53), K45 (= K58), Q98 (= Q111), P100 (= P113), A101 (= A114), K123 (= K136), R153 (= R166), H184 (= H198)
3e12A Cu2+ substituted aquifex aeolicus kdo8ps in complex with kdo8p (see paper)
49% identity, 90% coverage: 20:267/276 of query aligns to 6:251/258 of 3e12A
- binding copper (ii) ion: C10 (= C24), H184 (= H198), E216 (= E235), D227 (= D246)
- binding 3-deoxy-8-O-phosphono-D-manno-oct-2-ulosonic acid: K40 (= K53), K45 (= K58), N47 (= N60), R48 (= R61), S49 (= S62), Q98 (= Q111), K123 (= K136), H184 (= H198), D227 (= D246)
- binding phosphate ion: P100 (= P113), A101 (= A114), K123 (= K136), R153 (= R166)
2nx1A Structural and mechanistic changes along an engineered path from metallo to non-metallo kdo8p synthase (see paper)
49% identity, 90% coverage: 20:267/276 of query aligns to 6:256/263 of 2nx1A
- binding phosphoenolpyruvate: K40 (= K53), Q98 (= Q111), P100 (= P113), A101 (= A114), K123 (= K136), R153 (= R166), H184 (= H198)
- binding 5-O-phosphono-beta-D-ribofuranose: K45 (= K58), N47 (= N60), R48 (= R61), S49 (= S62), S196 (= S210), D232 (= D246)
1pcwA Aquifex aeolicus kdo8ps in complex with cadmium and app, a bisubstrate inhibitor (see paper)
49% identity, 90% coverage: 20:267/276 of query aligns to 6:250/257 of 1pcwA
- binding 1-deoxy-6-o-phosphono-1-[(phosphonomethyl)amino]-l-threo-hexitol: K45 (= K58), N47 (= N60), R48 (= R61), S49 (= S62), P100 (= P113), A101 (= A114), K123 (= K136), R153 (= R166), H184 (= H198), Q187 (= Q201), D226 (= D246)
1pe1A Aquifex aeolicus kdo8ps in complex with cadmium and 2-pga (see paper)
49% identity, 90% coverage: 20:267/276 of query aligns to 7:251/258 of 1pe1A
1pckA Aquifex aeolicus kdo8ps in complex with z-methyl-pep (see paper)
49% identity, 90% coverage: 20:267/276 of query aligns to 6:251/258 of 1pckA
2nx3D Structural and mechanistic changes along an engineered path from metallo to non-metallo kdo8p synthase (see paper)
49% identity, 90% coverage: 20:267/276 of query aligns to 5:255/262 of 2nx3D
- binding (2r,4r,5r,6r,7r)-2,4,5,6,7-pentahydroxy-2,8-bis(phosphonooxy)octanoic acid: N9 (≠ C24), K44 (= K58), N46 (= N60), R47 (= R61), S48 (= S62), Q97 (= Q111), P99 (= P113), A100 (= A114), K122 (= K136), R152 (= R166), H183 (= H198), K194 (≠ V209), S195 (= S210), D231 (= D246)
1jcxA Aquifex aeolicus kdo8p synthase in complex with api and cadmium (see paper)
48% identity, 90% coverage: 20:267/276 of query aligns to 6:248/255 of 1jcxA
- binding {[(2,2-dihydroxy-ethyl)-(2,3,4,5-tetrahydroxy-6-phosphonooxy-hexyl)-amino]-methyl}-phosphonic acid: K40 (= K53), K45 (= K58), N47 (= N60), R48 (= R61), S49 (= S62), Q98 (= Q111), P100 (= P113), A101 (= A114), K123 (= K136), R153 (= R166), H184 (= H198), Q187 (= Q201), F211 (= F233), E213 (= E235)
3undB Substrate-bound crystal structure of 2-dehydro-3-deoxyphosphooctonate aldolase from burkholderia pseudomallei (see paper)
46% identity, 91% coverage: 16:267/276 of query aligns to 17:272/284 of 3undB
- binding arabinose-5-phosphate: N27 (≠ C24), K61 (= K58), N63 (= N60), R64 (= R61), S65 (= S62), H203 (= H198), Q206 (= Q201), R208 (≠ P203), A214 (≠ V209), S215 (= S210), C250 (≠ S245), D251 (= D246)
- binding 3-deoxy-8-O-phosphono-D-manno-oct-2-ulosonic acid: K56 (= K53), K61 (= K58), N63 (= N60), R64 (= R61), S65 (= S62), K139 (= K136), H203 (= H198), R208 (≠ P203), A214 (≠ V209), S215 (= S210), D251 (= D246)
- binding phosphoenolpyruvate: K56 (= K53), Q114 (= Q111), P116 (= P113), A117 (= A114), K139 (= K136), R169 (= R166), H203 (= H198)
1g7vA Crystal structures of kdo8p synthase in its binary complexes with the mechanism-based inhibitor (see paper)
39% identity, 95% coverage: 5:267/276 of query aligns to 7:271/284 of 1g7vA
- active site: N26 (≠ C24), K60 (= K58), D250 (= D246)
- binding {[(2,2-dihydroxy-ethyl)-(2,3,4,5-tetrahydroxy-6-phosphonooxy-hexyl)-amino]-methyl}-phosphonic acid: N62 (= N60), R63 (= R61), F117 (= F115), R168 (= R166), H202 (= H198), Q205 (= Q201), A247 (= A243), G251 (≠ A247), P252 (≠ A248)
1g7uA Crystal structures of kdo8p synthase in its binary complex with substrate phosphoenol pyruvate (see paper)
39% identity, 95% coverage: 5:267/276 of query aligns to 7:271/284 of 1g7uA
4z1dA Structure of pep and zinc bound kdo8ps from h.Pylori (see paper)
43% identity, 90% coverage: 19:266/276 of query aligns to 4:252/256 of 4z1dA
1x6uA Kdo8p synthase in it's binary complex with the product kdo8p (see paper)
38% identity, 95% coverage: 5:267/276 of query aligns to 7:259/272 of 1x6uA