SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012467397.1 NCBI__GCF_000020465.1:WP_012467397.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
55% identity, 100% coverage: 1:397/397 of query aligns to 1:396/654 of P36204

query
sites
P36204

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R36 (= R37) binding substrate
R118 (= R119) binding substrate
R151 (= R152) binding substrate

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
55% identity, 99% coverage: 2:396/397 of query aligns to 1:394/398 of 1vpeA

query
sites
1vpeA

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active site: R35 (= R37), K196 (= K199), G353 (= G355), G376 (= G378)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G197), A195 (≠ S198), K196 (= K199), K200 (= K203), G218 (= G221), A219 (= A222), N316 (= N318), P318 (= P320), G320 (= G322), V321 (= V323), E323 (= E325), G352 (= G354), G353 (= G355), D354 (= D356), S355 (= S357)

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
54% identity, 100% coverage: 1:397/397 of query aligns to 1:393/394 of P40924

query
sites
P40924

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S183 (≠ Q185) modified: Phosphoserine
T299 (= T301) modified: Phosphothreonine

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
55% identity, 100% coverage: 1:397/397 of query aligns to 1:393/394 of 1phpA

query
sites
1phpA

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I

I

L

L

M

A

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

K

K

P

V

N

V

Q

E

D

E

F

L

S

R

L

L

T

D

P

A

A

V

A

A

E

K

R

R

L

L

S

G

E

E

L

L

L

L

D

E

C

R

P

P

V

V

I

A

M

K

A

T

G

N

D

E

C

A

I

V

G

G

T

D

E

E

V

V

M

K

Q

A

Q

A

V

V

L

D

A

R

L

L

Q

N

D

E

G

G

E

D

V

V

I

L

M

L

L

L

E

E

N

N

L

V

R

R

F

F

H

Y

P

P

E

G

E

E

E

E

A

K

N

N

D

D

P

P

D

E

F

L

A

A

K

K

E

A

L

F

A

A

S

E

L

L

G

A

E

D

I

L

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

H

H

A

A

S

S

T

T

E

E

G

G

I

I

T

A

R

H

Y

Y

V

L

Q

-

T

P

A

A

V

V

A

A

G

G

Y

F

L

L

I

M

E

E

K

K

E

E

L

L

M

E

Y

V

L

L

G

G

K

K

A

A

L

L

Q

S

E

N

P

P

E

D

R

R

P

P

F

F

V

T

A

A

I

I

L

I

G

G

G
|
G

S

A

K
|
K

I

V

S

K

G

D

K
|
K

I

I

D

G

V

V

L

I

E

D

N

N

L

L

F

L

N

E

K

K

V

V

D

D

T

N

V

L

L

I

I

I

G

G

G
|
G

A
x
G

M

L

V

A

F

Y

T

T

F

F

F

V

K

K

A

A

Q

L

G

G

L

H

G

D

T

V

G

G

N

K

S

S

L
|
L

V

L

E

E

E

E

N

D

K

K

L

I

E

E

L

L

A

A

L

K

S

S

L

F

I

M

E

E

Q

K

A

A

A

K

R

E

K

K

N

G

I

V

K

R

L

F

L

Y

L

M

P

P

Q

V

D

D

I

V

I

V

I

V

A

A

P

D

E

R

I

F

S

A

A

N

D

D

A

A

E

N

S

T

M

K

A

V

V

V

A

P

V

I

N

D

A

A

I

I

P

P

D

A

G

D

M

W

I

S

G

A

V

L

D

D

I

I

G

G

P

P

E

K

T

T

R

R

A

E

A

L

Y

Y

R

R

Q

D

E

V

I

I

L

R

G

E

A

S

R

K

T

L

V

V

L

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

I

M

D

D

R

A

F

F

A

A

E

H

G

G

T

T

I

K

A

A

I

I

A

A

E

E

A

A

M

L

A

-

D

-

A

A

T

E

A

A

A

L

G

D

A

T

T

Y

T

S

I

V

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

A

A

A

A

A

A

V

V

A

E

K

K

A

F

G

G

L

L

A

A

D

D

Q

K

I

M

T

D

H

H

I

I

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

F

L

M

E

E

G

G

K

K

E

Q

L

L

P

P

G

G

I

V

A

V

A

A

L

L

N

E

D

D

active site: R36 (= R37), K197 (= K199), G351 (= G355), G374 (= G378)
binding adenosine-5'-diphosphate: G195 (= G197), K201 (= K203), G219 (= G221), G220 (≠ A222), L237 (= L239), N316 (= N318), P318 (= P320), G320 (= G322), V321 (= V323), E323 (= E325), G350 (= G354), D352 (= D356), S353 (= S357)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
55% identity, 100% coverage: 1:397/397 of query aligns to 1:393/394 of P18912

query
sites
P18912

M

M

Q

N

K

K

K

K

T

T

L

I

S

R

D

D

I

V

T

D

I

V

Q

R

G

G

K

K

R

R

V

V

L

F

M

C

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

E

E

Q

K

G

N

A

I

I

T

T

D

D

D

D

K

T

R

R

I

I

V

R

E

A

A

A

L

L

P

P

S

T

I

I

K

R

K

Y

I

L

I

I

E

E

N

H

G

G

G

A

R

K

L

V

I

I

L

L

M

A

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

K

K

P

V

N

V

Q

E

D

E

F

L

S

R

L

L

T

D

P

A

A

V

A

A

E

K

R

R

L

L

S

G

E

E

L

L

L

L

D

E

C

R

P

P

V

V

I

A

M

K

A

T

G

N

D

E

C

A

I

V

G

G

T

D

E

E

V

V

M

K

Q

A

Q

A

V

V

L

D

A

R

L

L

Q

N

D

E

G

G

E

D

V

V

I

L

M

L

L

L

E

E

N

N

L

V

R

R

F

F

H

Y

P

P

E

G

E

E

E

E

A

K

N

N

D

D

P

P

D

E

F

L

A

A

K

K

E

A

L

F

A

A

S

E

L

L

G

A

E

D

I

L

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

H

H

A

A

S

S

T

T

E

E

G

G

I

I

T

A

R

H

Y

Y

V

L

Q

-

T

P

A

A

V

V

A

A

G

G

Y

F

L

L

I

M

E

E

K

K

E

E

L

L

M

E

Y

V

L

L

G

G

K

K

A

A

L

L

Q

S

E

N

P

P

E

D

R

R

P

P

F

F

V

T

A

A

I

I

L

I

G

G

G

G

S

A

K

K

I

V

S

K

G

D

K
|
K

I

I

D

G

V

V

L

I

E

D

N

N

L

L

F

L

N

E

K

K

V

V

D

D

T

N

V

L

L

I

I

I

G

G

G

G

A

G

M

L

V

A

F

Y

T

T

F

F

F

V

K

K

A

A

Q

L

G

G

L

H

G

D

T

V

G

G

N

K

S

S

L

L

V

L

E

E

E

E

N

D

K

K

L

I

E

E

L

L

A

A

L

K

S

S

L

F

I

M

E

E

Q

K

A

A

A

K

R

E

K

K

N

G

I

V

K

R

L

F

L

Y

L

M

P

P

Q

V

D

D

I

V

I

V

I

V

A

A

P

D

E

R

I

F

S

A

A

N

D

D

A

A

E

N

S

T

M

K

A

V

V

V

A

P

V

I

N

D

A

A

I

I

P

P

D

A

G

D

M

W

I

S

G

A

V

L

D

D

I

I

G

G

P

P

E

K

T

T

R

R

A

E

A

L

Y

Y

R

R

Q

D

E

V

I

I

L

R

G

E

A

S

R

K

T

L

V

V

L

V

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

I

M

D

D

R

A

F

F

A

A

E

H

G

G

T

T

I

K

A

A

I

I

A

A

E

E

A

A

M

L

A

-

D

-

A

A

T

E

A

A

A

L

G

D

A

T

T

Y

T

S

I

V

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

A

A

A

A

A

A

V

V

A

E

K

K

A

F

G

G

L

L

A

A

D

D

Q

K

I

M

T

D

H

H

I

I

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

F

F

L

M

E

E

G

G

K

K

E

Q

L

L

P

P

G

G

I

V

A

V

A

A

L

L

N

E

D

D

K201 (= K203) binding ATP
N316 (= N318) binding ATP
E323 (= E325) binding ATP
GGDS 350:353 (= GGDS 354:357) binding ATP

3zlbA Crystal structure of phosphoglycerate kinase from streptococcus pneumoniae (see paper)
52% identity, 100% coverage: 1:397/397 of query aligns to 1:397/398 of 3zlbA

query
sites
3zlbA

M
|
M

Q

A

K

K

K

L

T

T

L

V

S

K

D
|
D

I

V

T

D

I

L

Q

K

G

G

K

K

R

K

V

V

L

L

M

V

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

L

D

-

E

K

E

D

K

G

N

V

I

I

T

T

D

N

D

D

K

N

R
|
R

I

I

V

T

E

A

A

A

L

L

P

P

S

T

I

I

K

K

K

Y

I

I

I

I

E

E

N

Q

G

G

G

G

R

R

L

A

I

I

L

L

M

F

S

S

H

H

L

L

G

G

R

R

P

V

K

K

G

E

K

E

P

S

N

D

Q

K

-

A

D

G

F

K

S

S

L

L

T

A

P

P

A

V

A

A

E

A

R

D

L

L

S

A

E

A

L

K

L

L

D

G

C

Q

P

D

V

V

I

V

M

F

A

P

G

G

D

V

C

T

I

R

G

G

T

A

E

E

V

L

M

E

Q

A

Q

A

V

I

L

N

A

A

L

L

Q

E

D

D

G

G

E

Q

V

V

I

L

M

L

L

V

E

E

N

N

L

T

R

R

F

Y

H

E

-

D

-

V

-

D

-

G

P

K

E

K

E

E

E

S

A

K

N

N

D

D

P

P

D

E

F

L

A

G

K

K

E

Y

L

W

A

A

S

S

L

L

G

G

E

D

-

G

I

I

Y

F

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

A

S

S

T

N

E

V

G

G

I

I

T

S

R

A

Y

N

V

V

Q

E

T

K

A

A

V

V

A

A

G

G

Y

F

L

L

I

L

E

E

K

N

E

E

L

I

M

A

Y

Y

L

I

G

Q

K

E

A

A

L

V

Q

E

E

T

P

P

E

E

R

R

P

P

F

F

V

V

A

A

I

I

L

L

G

G

G
|
G

S
|
S

K
|
K

I

V

S

S

G

D

K

K

I

I

D

G

V

V

L

I

E

E

N

N

L

L

F

L

N

E

K

K

V

A

D

D

T

K

V

V

L

L

I

I

G

G

G
|
G

A
x
G

M

M

V

T

F

Y

T

T

F

F

F

Y

K

K

A

A

Q

Q

G

G

L

I

G

E

T

I

G

G

N

N

S

S

L

L

V

V

E

E

E

E

N

D

K

K

L

L

E

D

L

V

A

A

L

K

S

A

L

L

I

L

E

E

Q

K

A

A

A

-

R

-

K

-

N

N

I

G

K

K

L

L

L

I

L

L

P

P

Q

V

D

D

I

S

I

K

I

E

A

A

P

N

E

A

I

F

S

A

A

G

D

Y

A

T

E

E

S

V

M

R

A

D

V

T

A

E

V

G

N

E

A

A

I

V

P

S

D

E

G

G

M

F

I

L

G

G

V

L

D

D

I

I

G

G

P

P

E

K

T

S

R

I

A

A

A

K

Y

F

R

D

Q

E

E

A

I

L

L

T

G

G

A

A

R

K

T

T

V

V

L

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

I

N

D

P

R

D

F

F

A

Q

E

A

G

G

T

T

I

I

A

G

I

V

A

M

E

D

A

A

M

I

A

-

D

-

A

V

T

K

A

Q

A

P

G

G

A

V

T

K

T

S

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

A

A

A

A

A

A

V

A

A

I

K

N

A

L

G

G

L

R

A

A

D

D

Q

K

I

F

T

S

H

W

I

I

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

M

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

L

A

A

A

A

L

L

N

T

D

E

active site: R36 (= R37), K204 (= K199), G355 (= G355), G378 (= G378)
binding phosphoaminophosphonic acid-adenylate ester: G202 (= G197), S203 (= S198), G226 (= G221), G227 (≠ A222), N320 (= N318), P322 (= P320), G324 (= G322), V325 (= V323), E327 (= E325), G354 (= G354), G355 (= G355), D356 (= D356), S357 (= S357)
binding magnesium ion: M1 (= M1), D8 (= D8)

Sites not aligning to the query:

binding magnesium ion: 398

P00560 Phosphoglycerate kinase; EC 2.7.2.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
48% identity, 100% coverage: 2:397/397 of query aligns to 5:414/416 of P00560

query
sites
P00560

Q

S

K

K

K

L

T

S

L

V

S

Q

D

D

I

L

T

D

I

L

Q

K

G

D

K

K

R

R

V

V

L

F

M

I

R
|
R

V

V

D

D

F

F

N

N

V

V

P

P

L

L

D

D

E

G

E

K

K

K

N

-

I

I

T

T

D

S

D

N

K

Q

R
|
R

I

I

V

V

E

A

A

A

L

L

P

P

S

T

I

I

K

K

K

Y

I

V

I

L

E

E

N

H

G

H

G

P

R

R

-

Y

L

V

I

V

L

L

M

A

S

S

H

H

L

L

G

G

R

R

P

P

K

N

G

G

K

E

P

R

N

N

Q

E

D

K

F

Y

S

S

L

L

T

A

P

P

A

V

A

A

E

K

R

E

L

L

S

Q

E

S

L

L

L

L

D

G

C

K

P

D

V

V

I

T

M

F

A

L

G

N

D

D

C

C

I

V

G

G

T

P

E

E

V

V

M

E

Q

A

Q

A

V

V

L

K

A

A

L

S

Q

A

D

P

G

G

E

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R
|
R

F

Y

H

H

P

I

E

E

E

E

E

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

A

A

N

S

D

K

P

E

D

D

-

V

-

Q

-

K

F

F

A

R

K

H

E

E

L

L

A

S

S

S

L

L

G

A

E

D

I

V

Y

Y

V

I

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

A

A

H

H

A

S

S

S

T

M

E

V

G

G

I

F

T

D

R

-

Y

-

V

L

Q

P

T

Q

A

R

V

A

A

A

G

G

Y

F

L

L

I

L

E

E

K

K

E

E

L

L

M

K

Y

Y

L

F

G

G

K

K

A

A

L

L

Q

E

E

N

P

P

E

T

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G

G

S

A

K

K

I

V

S

A

G

D

K

K

I

I

D

Q

V

L

L

I

E

D

N

N

L

L

F

L

N

D

K

K

V

V

D

D

T

S

V

I

L

I

I

I

G

G

G

G

A

G

M

M

V

A

F

F

T

T

F

F

F

K

K

K

A

V

-

L

Q

E

G

N

L

T

G

E

T

I

G

G

N

D

S

S

L

I

V

F

E

D

E

K

N

A

K

G

L

A

E

E

L

I

A

V

L

P

S

K

L

L

I

M

E

E

Q

K

A

A

A

K

R

A

K

K

N

G

I

V

K

E

L

V

L

V

L

L

P

P

Q

V

D

D

I

F

I

I

I

I

A

A

P

D

E

A

I

F

S

S

A

A

D

D

A

A

E

N

S

T

M

K

A

T

V

V

A

T

-

D

V

K

N

E

A

G

I

I

P

P

D

A

G

G

M

W

I

Q

G

G

V

L

D

D

I

N

G

G

P

P

E

E

T

S

R

R

A

K

A

L

Y

F

R

A

Q

A

E

T

I

V

L

A

G

K

A

A

R

K

T

T

V

I

L

V

W

W

N

N

G

G

P

P

M

P

G

G

V

V

F

F

E

E

I

F

D

E

R

K

F

F

A

A

E

A

G

G

T

T

I

K

A

A

I

L

A

L

E

D

A

E

M

V

A

V

D

K

A

S

T

S

A

A

A

A

G

G

A

N

T

T

T

V

I

I

I

I

G

G

G

G

G

G

D

D

S

T

A

A

A

T

A

V

V

A

A

K

K

K

A

Y

G

G

L

V

A

T

D

D

Q

K

I

I

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

E

L

L

P

P

G

G

I

V

A

A

A

F

L

L

N

S

D

E

R22 (= R19) mutation to K: 2-fold reduction of Vmax.; mutation to M: 7-fold reduction of Vmax.
R39 (= R37) binding substrate
R122 (= R119) binding substrate
R169 (= R152) binding substrate

1qpgA 3-phosphoglycerate kinase, mutation r65q (see paper)
48% identity, 100% coverage: 2:397/397 of query aligns to 4:413/415 of 1qpgA

query
sites
1qpgA

Q

S

K

K

K

L

T

S

L

V

S

Q

D

D

I

L

T

D

I

L

Q

K

G

D

K

K

R

R

V

V

L

F

M

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

L

D

D

E

G

E

K

K

K

N

-

I

I

T

T

D

S

D

N

K

Q

R
|
R

I

I

V

V

E

A

A

A

L

L

P

P

S

T

I

I

K

K

K

Y

I

V

I

L

E

E

N

H

G

H

G

P

R

R

-

Y

L

V

I

V

L

L

M

A

S

S

H

H

L

L

G

G

R

Q

P

P

K

N

G

G

K

E

P

R

N

N

Q

E

D

K

F

Y

S

S

L

L

T

A

P

P

A

V

A

A

E

K

R

E

L

L

S

Q

E

S

L

L

L

L

D

G

C

K

P

D

V

V

I

T

M

F

A

L

G

N

D

D

C

C

I

V

G

G

T

P

E

E

V

V

M

E

Q

A

Q

A

V

V

L

K

A

A

L

S

Q

A

D

P

G

G

E

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

Y

H

H

P

I

E

E

E

E

E

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

A

A

N

S

D

K

P

E

D

D

-

V

-

Q

-

K

F

F

A

R

K

H

E

E

L

L

A

S

S

S

L

L

G

A

E

D

I

V

Y

Y

V

I

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

M

E

V

G

G

I

F

T

D

R

-

Y

-

V

L

Q

P

T

Q

A

R

V

A

A

A

G

G

Y

F

L

L

I

L

E

E

K

K

E

E

L

L

M

K

Y

Y

L

F

G

G

K

K

A

A

L

L

Q

E

E

N

P

P

E

T

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G

G

S

A

K
|
K

I

V

S

A

G

D

K

K

I

I

D

Q

V

L

L

I

E

D

N

N

L

L

F

L

N

D

K

K

V

V

D

D

T

S

V

I

L

I

I

I

G

G

G
|
G

A
x
G

M

M

V

A

F

F

T

T

F

F

F

K

K

K

A

V

-

L

Q

E

G

N

L

T

G

E

T

I

G

G

N

D

S

S

L

I

V

F

E

D

E

K

N

A

K

G

L

A

E

E

L

I

A

V

L

P

S

K

L

L

I

M

E

E

Q

K

A

A

A

K

R

A

K

K

N

G

I

V

K

E

L

V

L

V

L

L

P

P

Q

V

D

D

I

F

I

I

I

I

A

A

P

D

E

A

I

F

S

S

A

A

D

D

A

A

E

N

S

T

M

K

A

T

V

V

A

T

-

D

V

K

N

E

A

G

I

I

P

P

D

A

G

G

M

W

I

Q

G

G

V

L

D

D

I

N

G

G

P

P

E

E

T

S

R

R

A

K

A

L

Y

F

R

A

Q

A

E

T

I

V

L

A

G

K

A

A

R

K

T

T

V

I

L

V

W

W

N
|
N

G

G

P
|
P

M

P

G
|
G

V
|
V

F
|
F

E
|
E

I

F

D

E

R

K

F

F

A

A

E

A

G

G

T

T

I

K

A

A

I

L

A

L

E

D

A

E

M

V

A

V

D

K

A

S

T

S

A

A

A

A

G

G

A

N

T

T

T

V

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

V

V

A

A

K

K

K

A

Y

G

G

L

V

A

T

D

D

Q

K

I

I

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

E

L

L

P

P

G

G

I

V

A

A

A

F

L

L

N

S

D

E

active site: R38 (= R37), K213 (= K199), G371 (= G355), G394 (= G378)
binding magnesium-5'-adenyly-imido-triphosphate: G235 (= G221), G236 (≠ A222), N334 (= N318), P336 (= P320), G338 (= G322), V339 (= V323), F340 (= F324), E341 (= E325), G370 (= G354), G371 (= G355), D372 (= D356), T373 (≠ S357)

16pkA Phosphoglycerate kinase from trypanosoma brucei bisubstrate analog (see paper)
47% identity, 100% coverage: 2:397/397 of query aligns to 1:414/415 of 16pkA

query
sites
16pkA

Q

E

K

K

K

K

T

S

L

I

S

N

D

E

I

C

T

D

I

L

Q

K

G

G

K

K

R

K

V

V

L

L

M

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

V

D

K

E

N

E

G

K

K

N

-

I

I

T

T

D

N

D

D

K

Y

R
|
R

I

I

V

R

E

S

A

A

L

L

P

P

S

T

I

L

K

K

K

K

I

V

I

L

E

T

N

E

G

G

G

G

R

S

L

C

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

K

I

P

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

-

G

-

F

N

Q

Q

Q

D

K

F

A

S

T

L

L

T

K

P

P

A

V

A

A

E

K

R

R

L

L

S

S

E

E

L

L

L

L

D

L

C

R

P

P

V

V

I

T

M

F

A

A

G

P

D

D

C

C

I

L

G

-

T

-

E

N

V

A

M

A

Q

D

Q

V

V

V

L

S

A

K

L

M

Q

S

D

P

G

G

E

D

V

V

I

V

M

L

L

L

E

E

N

N

L

V

R

R

F

F

H

Y

P

K

E

E

E

E

-

G

-

S

-

K

E

K

A

A

N

K

D

D

P

R

D

E

-

A

F

M

A

A

K

K

E

I

L

L

A

A

S

S

L

Y

G

G

E

D

I

V

Y

Y

V

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

D

H

S

A

A

S

T

T

M

E

T

G

G

I

I

T

P

R

K

Y

I

V

L

Q

G

T

N

A

G

V

A

A

A

G

G

Y

Y

L

L

I

M

E

E

K

K

E

E

L

I

M

S

Y

Y

L

F

G

A

K

K

A

V

L

L

Q

G

E

N

P

P

E

P

R

R

P

P

F

L

V

V

A

A

I

I

L

V

G

G

G
|
G

S
x
A

K
|
K

I

V

S

S

G

D

K
|
K

I

I

D

Q

V

L

L

L

E

D

N

N

L

M

F

L

N

Q

K

R

V

I

D

D

T

Y

V

L

L

L

I

I

G

G

G

G

A
|
A

M

M

V

A

F
x
Y

T

T

F

F

F

L

K

K

A

A

Q

Q

G

G

L

Y

G

S

T

I

G

G

N

K

S

S

L

K

V

C

E

E

E

E

N

S

K

K

L

L

E

E

L

F

A

A

L

R

S

S

L

L

I

L

E

K

Q

K

A

A

A

E

R

D

K

R

N

K

I

V

K

Q

L

V

L

I

L

L

P

P

Q

I

D

D

I

H

I

V

I

C

A

H

P

T

E

E

I

F

S

K

A

A

D

V

A

D

E

S

S

P

M

L

A

I

V

T

A

E

V

D

N

Q

A

N

I

I

P

P

D

E

G

G

M

H

I

M

G

A

V
x
L

D

D

I

I

G

G

P

P

E

K

T

T

R

I

A

E

A

K

Y

Y

R

V

Q

Q

E

T

I

I

L

G

G

K

A

C

R

K

T

S

V

A

L

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

I

M

D

V

R

P

F

Y

A

S

E

K

G

G

T

T

I

F

A

A

I

I

A

A

E

K

A

A

M

M

A

G

D

R

A

G

T

T

A

H

-

E

A

H

G

G

A

L

T

M

T

S

I

I

I

I

G

G

G

G

G
|
G

D

D

S

S

A

A

A

S

A

A

V

A

A

E

K

L

A

S

G

G

L

E

A

A

D

K

Q

R

I

M

T

S

H

H

I

V

S

S

T

T

G
|
G

G
|
G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

T

L

L

P

P

G

G

I

V

A

T

A

V

L

L

N

D

D

D

active site: R35 (= R37), K215 (= K199), G372 (= G355), G395 (= G378)
binding 1,1,5,5-tetrafluorophosphopentylphosphonic acid adenylate ester: G213 (= G197), A214 (≠ S198), K219 (= K203), A238 (= A222), Y241 (≠ F225), L311 (≠ V295), P336 (= P320), G338 (= G322), V339 (= V323), E341 (= E325), G393 (= G376), G394 (= G377), G395 (= G378)

13pkA Ternary complex of phosphoglycerate kinase from trypanosoma brucei (see paper)
47% identity, 100% coverage: 2:397/397 of query aligns to 1:414/415 of 13pkA

query
sites
13pkA

Q

E

K

K

K

K

T

S

L

I

S

N

D

E

I

C

T

D

I

L

Q

K

G

G

K

K

R

K

V

V

L

L

M

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

V

D

K

E

N

E

G

K

K

N

-

I

I

T

T

D

N

D

D

K

Y

R
|
R

I

I

V

R

E

S

A

A

L

L

P

P

S

T

I

L

K

K

K

K

I

V

I

L

E

T

N

E

G

G

G

G

R

S

L

C

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

K

I

P

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

-

G

-

F

N

Q

Q

Q

D

K

F

A

S

T

L

L

T

K

P

P

A

V

A

A

E

K

R

R

L

L

S

S

E

E

L

L

L

L

D

L

C

R

P

P

V

V

I

T

M

F

A

A

G

P

D

D

C

C

I

L

G

-

T

-

E

N

V

A

M

A

Q

D

Q

V

V

V

L

S

A

K

L

M

Q

S

D

P

G

G

E

D

V

V

I

V

M

L

L

L

E

E

N

N

L

V

R

R

F

F

H

Y

P

K

E

E

E

E

-

G

-

S

-

K

E

K

A

A

N

K

D

D

P

R

D

E

-

A

F

M

A

A

K

K

E

I

L

L

A

A

S

S

L

Y

G

G

E

D

I

V

Y

Y

V

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

D

H

S

A

A

S

T

T

M

E

T

G

G

I

I

T

P

R

K

Y

I

V

L

Q

G

T

N

A

G

V

A

A

A

G

G

Y

Y

L

L

I

M

E

E

K

K

E

E

L

I

M

S

Y

Y

L

F

G

A

K

K

A

V

L

L

Q

G

E

N

P

P

E

P

R

R

P

P

F

L

V

V

A

A

I

I

L

V

G

G

G
|
G

S
x
A

K
|
K

I

V

S

S

G

D

K
|
K

I

I

D

Q

V

L

L

L

E

D

N

N

L

M

F

L

N

Q

K

R

V

I

D

D

T

Y

V

L

L

L

I

I

G

G

G

G

A

A

M

M

V

A

F

Y

T

T

F

F

F

L

K

K

A

A

Q

Q

G

G

L

Y

G

S

T

I

G

G

N

K

S

S

L

K

V

C

E

E

E

E

N

S

K

K

L

L

E

E

L

F

A

A

L

R

S

S

L

L

I

L

E

K

Q

K

A

A

A

E

R

D

K

R

N

K

I

V

K

Q

L

V

L

I

L

L

P

P

Q

I

D

D

I

H

I

V

I

C

A

H

P

T

E

E

I

F

S

K

A

A

D

V

A

D

E

S

S

P

M

L

A

I

V

T

A

E

V

D

N

Q

A

N

I

I

P

P

D

E

G

G

M

H

I

M

G

A

V
x
L

D

D

I

I

G

G

P

P

E

K

T

T

R

I

A

E

A

K

Y

Y

R

V

Q

Q

E

T

I

I

L

G

G

K

A

C

R

K

T

S

V

A

L

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

I

M

D

V

R

P

F

Y

A

S

E

K

G

G

T

T

I

F

A

A

I

I

A

A

E

K

A

A

M

M

A

G

D

R

A

G

T

T

A

H

-

E

A

H

G

G

A

L

T

M

T

S

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

A

A

A

S

A

A

V

A

A

E

K

L

A

S

G

G

L

E

A

A

D

K

Q

R

I

M

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

T

L

L

P

P

G

G

I

V

A

T

A

V

L

L

N

D

D

D

active site: R35 (= R37), K215 (= K199), G372 (= G355), G395 (= G378)
binding adenosine-5'-diphosphate: G213 (= G197), A214 (≠ S198), K219 (= K203), L311 (≠ V295), P336 (= P320), G338 (= G322), V339 (= V323), E341 (= E325), G371 (= G354), D373 (= D356), S374 (= S357)

P07378 Phosphoglycerate kinase, glycosomal; Phosphoglycerate kinase C; EC 2.7.2.3 from Trypanosoma brucei brucei (see 2 papers)
46% identity, 100% coverage: 2:397/397 of query aligns to 5:418/440 of P07378

query
sites
P07378

Q

E

K

K

K

K

T

S

L

I

S

N

D

E

I

C

T

D

I

L

Q

K

G

G

K

K

R

K

V

V

L

L

M

I

R

R

V

V

D
|
D

F
|
F

N
|
N

V

V

P

P

L

V

D

K

E

N

E

G

K

K

N

-

I

I

T

T

D

N

D

D

K

Y

R
|
R

I

I

V

R

E

S

A

A

L

L

P

P

S

T

I

L

K

K

K

K

I

V

I

L

E

T

N

E

G

G

G

G

R

S

L

C

I

V

L

L

M

M

S

S

H
|
H

L
|
L

G
|
G

R
|
R

P

P

K

K

G

G

K

I

P

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

-

G

-

F

N

Q

Q

Q

D

K

F

A

S

T

L

L

T

K

P

P

A

V

A

A

E

K

R

A

L

L

S

S

E

E

L

L

L

L

D

L

C

R

P

P

V

V

I

T

M

F

A

A

G

P

D

D

C

C

I

L

G

-

T

-

E

N

V

A

M

A

Q

D

Q

V

V

V

L

S

A

K

L

M

Q

S

D

P

G

G

E

D

V

V

I

V

M

L

L

L

E

E

N

N

L

V

R
|
R

F

F

H

Y

P

K

E

E

E

E

-

G

-

S

-

K

E

K

A

A

N

K

D

D

P

R

D

E

-

A

F

M

A

A

K

K

E

I

L

L

A

A

S

S

L

Y

G

G

E

D

I

V

Y

Y

V

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

A

D

H

S

A

A

S

T

T

M

E

T

G

G

I

I

T

P

R

K

Y

I

V

L

Q

G

T

N

A

G

V

A

A

A

G

G

Y

Y

L

L

I

M

E

E

K

K

E

E

L

I

M

S

Y

Y

L

F

G

A

K

K

A

V

L

L

Q

G

E

N

P

P

E

P

R

R

P

P

F

L

V

V

A

A

I

I

L

V

G

G

G

G

S

A

K

K

I

V

S

S

G

D

K
|
K

I

I

D

Q

V

L

L

L

E

D

N

N

L

M

F

L

N

Q

K

R

V

I

D

D

T

Y

V

L

L

L

I

I

G

G

G

G

A

A

M

M

V

A

F

Y

T

T

F

F

F

L

K

K

A

A

Q

Q

G

G

L

Y

G

S

T

I

G

G

N

K

S

S

L

K

V

C

E

E

E

E

N

S

K

K

L

L

E

E

L

F

A

A

L

R

S

S

L

L

I

L

E

K

Q

K

A

A

A

E

R

D

K

R

N

K

I

V

K

Q

L

V

L

I

L

L

P

P

Q

I

D

D

I

H

I

V

I

C

A

H

P

T

E

E

I

F

S

K

A

A

D

V

A

D

E

S

S

P

M

L

A

I

V

T

A

E

V

D

N

Q

A

N

I

I

P

P

D

E

G

G

M

H

I

M

G

A

V

L

D

D

I

I

G

G

P

P

E

K

T

T

R

I

A

E

A

K

Y

Y

R

V

Q

Q

E

T

I

I

L

G

G

K

A

C

R

K

T

S

V

A

L

I

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

I

M

D

V

R

P

F

Y

A

S

E

K

G

G

T

T

I

F

A

A

I

I

A

A

E

K

A

A

M

M

A

G

D

R

A

G

T

T

A

H

-

E

A

H

G

G

A

L

T

M

T

S

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

A

A

A

S

A

A

V

A

A

E

K

L

A

S

G

G

L

E

A

A

D

K

Q

R

I

M

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

T

L

L

P

P

G

G

I

V

A

T

A

V

L

L

N

D

D

E

DFN 24:26 (= DFN 21:23) binding substrate
R39 (= R37) binding substrate
HLGR 62:65 (= HLGR 60:63) binding substrate
R135 (= R119) binding substrate
R172 (= R152) binding substrate
K223 (= K203) binding ATP
N338 (= N318) binding ATP
E345 (= E325) binding ATP
GGDS 375:378 (= GGDS 354:357) binding ATP

3pgkA The structure of yeast phosphoglycerate kinase at 0.25 nm resolution (see paper)
47% identity, 100% coverage: 2:397/397 of query aligns to 4:413/415 of 3pgkA

query
sites
3pgkA

Q

S

K

K

K

L

T

S

L

V

S

Q

D

D

I

L

T

D

I

L

Q

K

G

D

K

K

R

R

V

V

L

F

M

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

L

D

D

E

G

E

K

K

K

N

-

I

I

T

T

D

S

D

N

K

Q

R
|
R

I

I

V

V

E

A

A

A

L

L

P

P

S

T

I

I

K

K

K

Y

I

V

I

L

E

E

N

H

G

H

G

P

R

R

-

Y

L

V

I

V

L

L

M

A

S

S

H

H

L

L

G

G

R

R

P

P

K

N

G

G

K

E

P

R

N

N

Q

E

D

K

F

Y

S

S

L

L

T

A

P

P

A

V

A

A

E

K

R

E

L

L

S

Q

E

S

L

L

L

L

D

G

C

K

P

D

V

V

I

T

M

F

A

L

G

N

D

D

C

C

I

V

G

G

T

P

E

E

V

V

M

E

Q

A

Q

A

V

V

L

K

A

A

L

S

Q

A

D

P

G

G

E

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

Y

H

H

P

I

E

E

E

E

E

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

A

A

N

S

D

K

P

E

D

D

-

V

-

Q

-

K

F

F

A

R

K

H

E

E

L

L

A

S

S

S

L

L

G

A

E

D

I

V

Y

Y

V

I

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

M

E

V

G

G

I

F

T

D

R

-

Y

-

V

L

Q

P

T

Q

A

R

V

A

A

A

G

G

Y

F

L

L

I

L

E

E

K

K

E

E

L

L

M

K

Y

Y

L

F

G

G

K

K

A

A

L

L

Q

E

E

N

P

P

E

T

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

S
x
A

K
|
K

I

V

S

A

G

D

K

K

I

I

D

Q

V

L

L

I

E

D

N

N

L

L

F

L

N

D

K

K

V

V

D

D

T

S

V

I

L

I

I

I

G

G

G

G

A

G

M

M

V

A

F

F

T

T

F

F

F

K

K

K

A

V

-

L

Q

E

G

N

L

T

G

E

T

I

G

G

N

D

S

S

L

I

V

F

E

D

E

K

N

A

K

V

L

G

E

P

L

E

A

I

L

A

S

K

L

L

I

M

E

E

Q

K

A

A

A

K

R

A

K

K

N

G

I

V

K

E

L

V

L

V

L

L

P

P

Q

V

D

D

I

F

I

I

I

I

A

A

P

D

E

A

I
x
F

S

S

A

A

D

S

A

A

E

N

S

T

M

K

A

T

V

V

A

T

-

D

V

K

N

E

A

G

I

I

P

P

D

A

G

G

M

W

I

Q

G

G

V
x
L

D

D

I

N

G

G

P

P

E

E

T

S

R

R

A

K

A

L

Y

F

R

A

Q

A

E

T

I

V

L

A

G

K

A

A

R

T

T

V

V

I

L

L

W

W

N
|
N

G
|
G

P
|
P

M

P

G
|
G

V
|
V

F

F

E

E

I

F

D

E

R

K

F

F

A

A

E

A

G

G

T

T

I

K

A

A

I

L

A

L

E

D

A

E

M

V

A

V

D

K

A

S

T

S

A

A

A

A

G

G

A

N

T

T

T

V

I

I

I

I

G

G

G

G

G
|
G

D
|
D

S

T

A

A

A

T

A

V

V

A

A

K

K

K

A

Y

G

G

L

V

A

T

D

D

Q

K

I

I

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

E

L

L

P

P

G

G

I

V

A

A

A

F

L

L

N

S

D

E

active site: R38 (= R37), K213 (= K199), G371 (= G355), G394 (= G378)
binding adenosine-5'-triphosphate: G211 (= G197), A212 (≠ S198), K213 (= K199), F289 (≠ I274), L311 (≠ V295), N334 (= N318), G335 (= G319), P336 (= P320), G338 (= G322), V339 (= V323), D372 (= D356)

P09041 Phosphoglycerate kinase 2; Phosphoglycerate kinase, testis specific; EC 2.7.2.3 from Mus musculus (Mouse) (see paper)
45% identity, 99% coverage: 3:397/397 of query aligns to 6:416/417 of P09041

query
sites
P09041

K

K

K

L

T

T

L

L

S

D

D

K

I

V

T

D

I

L

Q

K

G

G

K

K

R

R

V

V

L

I

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

K

E

N

K

N

N

Q

I

I

T

T

D

N

D

N

K

Q

R

R

I

I

V

K

E

A

A

A

L

I

P

P

S

S

I

I

K

K

K

H

I

C

I

L

E

D

N

N

G

G

G

A

R

K

-

S

L

V

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

K

I

P

P

N

M

Q

P

D

D

-

K

F

Y

S

S

L

L

T

E

P

P

A

V

A

A

E

D

R

E

L

L

S

K

E

S

L

L

L

L

D

N

C

K

P

D

V

V

I

I

M

F

A

L

G

K

D

D

C

C

I

V

G

G

T

P

E

E

V

V

M

E

Q

Q

Q

A

V

C

L

A

A

N

L

P

Q

D

D

N

G

G

E

S

V

I

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

P

V

E

E

E

E

E

E

A

G

N

K

D

G

P

K

D

D

-

S

-

S

-

G

-

K

-

K

-

I

-

S

-

A

-

D

-

P

-

A

-

K

-

V

-

E

-

A

F

F

A

Q

K

A

E

S

L

L

A

S

S

K

L

L

G

G

E

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

T

E

V

G

G

I

V

T

N

R

-

Y

-

V

L

Q

P

T

Q

A

K

V

A

A

S

G

G

Y

F

L

L

I

M

E

K

K

K

E

E

L

L

M

D

Y

Y

L

F

G

S

K

K

A

A

L

L

Q

E

E

K

P

P

E

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G

G

S

A

K

K

I

V

S

K

G

D

K
|
K

I

I

D

Q

V

L

L

I

E

K

N

N

L

M

F

L

N

D

K

K

V

V

D

N

T

F

V

M

L

I

I

I

G

G

G

G

A

G

M

M

V

A

F

Y

T

T

F

F

F

L

K

K

A

E

-

L

Q

K

G

N

L

M

G

Q

T

I

G

G

N

A

S

S

L

L

V

F

E

D

E

E

N

E

K

G

L

A

E

T

L

I

A

V

L

K

S

E

L

I

I

M

E

E

Q

K

A

A

A

E

R

K

K

N

N

G

I

V

K

K

L

I

L

V

L

F

P

P

Q

V

D

D

I

F

I

V

I

T

A

G

P

D

E

K

I

F

S

D

A

E

D

N

A

A

E

K

-

V

S

G

M

Q

A

A

V

T

A

I

V

E

N

S

A

G

I

I

P

P

D

S

G

G

M

W

I

M

G
|
G

V

L

D

D

I

C

G

G

P

P

E

E

T

S

R

I

A

K

A

I

Y

N

R

A

Q

Q

E

I

I

V

L

A

G

Q

A

A

R

K

T

L

V

I

L

V

W

W

N

N

G

G

P

P

M

I

G

G

V

V

F

F

E
|
E

I

W

D

D

R

A

F

F

A

A

E

K

G

G

T

T

I

K

A

A

I

L

A

M

E

D

A

E

M

V

A

V

D

K

A

A

T

T

A

S

A

N

G

G

A

C

T

V

T

T

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

A

A

K

K

A

W

G

G

L

T

A

E

D

D

Q

K

I

V

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

I

L

L

P

P

G

G

I

V

A

E

A

A

L

L

N

S

D

N

K220 (= K203) binding ATP
G313 (= G294) binding ATP
E344 (= E325) binding ATP
GGDT 373:376 (≠ GGDS 354:357) binding ATP

O60101 Phosphoglycerate kinase; EC 2.7.2.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
46% identity, 99% coverage: 3:396/397 of query aligns to 6:412/414 of O60101

query
sites
O60101

K

K

K

L

T

A

L

I

S

T

D

D

I

V

T

D

I

L

Q

K

G

G

K

K

R

N

V

V

L

L

M

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

L

D

D

E

G

E

D

K

R

N

-

I

I

T

T

D

N

D

N

K

A

R

R

I

I

V

V

E

G

A

A

L

L

P

P

S

T

I

I

K

K

K

Y

I

A

I

L

E

E

N

Q

G

Q

G

P

R

K

-

A

L

V

I

I

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

N

G

G

K

A

P

R

N

V

Q

A

D

K

F
x
Y

S
|
S

L

L

T

K

P

P

A

V

A

A

E

A

R

E

L

L

S

S

E

K

L

L

L

L

D

G

C

K

P

P

V

V

I

K

M

F

A

L

G

D

D

D

C

C

I

V

G

G

T

P

E

E

V

V

M

E

Q

K

Q

A

V

C

L

K

A

E

L

A

Q

K

D

G

G

G

E

E

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

P

I

E

E

E

E

E

E

A

G

N

S

D

A

P

K

-

V

-

D

-

G

-

K

-

K

-

V

-

K

-

A

-

D

-

A

-
x
S

-

A

-

V

-

E

D

A

F

F

A

R

K

K

E

S

L

L

A

T

S

S

L

L

G

G

E

D

I

I

Y

F

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A
x
S

S
|
S

T

M

E

V

G

G

I

V

T

D

R

-

Y

-

V

-

Q

L

T

P

A

R

V

V

A
x
S

G

G

Y

F

L

L

I

M

E

K

K

K

E

E

L

L

M

D

Y

Y

L

F

G

S

K

K

A

A

L

L

Q

E

E

N

P

P

E

A

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G

G

S

A

K

K

I

V

S

A

G

D

K

K

I

I

D

Q

V

L

L

I

E

D

N

N

L

L

F

L

N

D

K

K

V

V

D

N

T

R

V

L

L

I

I

I

G

C

G

G

A

G

M

M

V

A

F

F

T

T

F

F

F

L

K

K

A

V

-

L

Q

N

G

G

L

M

G

K

T

I

G

G

N

D

S
|
S

L

L

V

F

E

D

E

E

N

A

K

G

L
x
S

E

K

L

N

A

V

L

E

S

S

L

M

I

M

E

A

Q

K

A

A

A

K

R

K

K

N

N

N

I

V

K

E

L

V

L

F

L

L

P

P

Q

V

D

D

I

F

I

V

I

T

A

A

P

D

E

K

I

F

S

D

A

K

D

D

A

A

E

K

-

V

S

G

M

S

A

A

V
x
T

A

A

V

E

N

E

A

G

I

I

P

P

D

D

G

G

M

W

I

M

G

G

V

L

D

D

I

C

G

G

P

P

E

K

T

S

R

S

A

A

A

K

Y

F

R

A

Q

E

E

V

I

I

L

T

G

T

A
x
S

R

K

T

T

V

I

L

V

W

W

N

N

G

G

P

P

M

A

G

G

V

V

F

F

E

E

I

F

D

D

R

N

F

F

A

A

E

K

G

G

T

T

I

K

A
x
S

I

M

A

L

E

D

A

A

M

C

A

V

D

K

A

T

T

C

A

E

A

A

G

G

A

N

T

V

T

V

I

I

I

V

G

G

G

G

G

G

D

D

S
x
T

A

A

A

T

A

V

V

A

A

K

K

K

A

Y

G

G

L

K

A

E

D

D

Q

A

I

L

T
x
S

H

H

I

V

S
|
S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

A

L

L

P

P

G

G

I

V

A

V

A

A

L

L

N
x
S

Y75 (≠ F72) modified: Phosphotyrosine
S76 (= S73) modified: Phosphoserine
S143 (vs. gap) modified: Phosphoserine
S172 (≠ A155) modified: Phosphoserine
S173 (= S156) modified: Phosphoserine
S183 (≠ A169) modified: Phosphoserine
S253 (= S238) modified: Phosphoserine
S260 (≠ L245) modified: Phosphoserine
T299 (≠ V283) modified: Phosphothreonine
S328 (≠ A312) modified: Phosphoserine
S351 (≠ A335) modified: Phosphoserine
T373 (≠ S357) modified: Phosphothreonine
S387 (≠ T371) modified: Phosphoserine
S390 (= S374) modified: Phosphoserine
S412 (≠ N396) modified: Phosphoserine

Sites not aligning to the query:

413 modified: Phosphoserine

2paaA Crystal structure of phosphoglycerate kinase-2 bound to atp and 3pg (see paper)
45% identity, 99% coverage: 3:397/397 of query aligns to 2:412/413 of 2paaA

query
sites
2paaA

K

K

K

L

T

T

L

L

S

D

D

K

I

V

T

D

I

L

Q

K

G

G

K

K

R

R

V

V

L

I

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

K

E

N

K

N

N

Q

I

I

T

T

D

N

D

N

K

Q

R
|
R

I

I

V

K

E

A

A

A

L

I

P

P

S

S

I

I

K

K

K

H

I

C

I

L

E

D

N

N

G

G

G

A

R

K

-

S

L

V

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

K

I

P

P

N

M

Q

P

D

D

-

K

F

Y

S

S

L

L

T

E

P

P

A

V

A

A

E

D

R

E

L

L

S

K

E

S

L

L

L

L

D

N

C

K

P

D

V

V

I

I

M

F

A

L

G

K

D

D

C

C

I

V

G

G

T

P

E

E

V

V

M

E

Q

Q

Q

A

V

C

L

A

A

N

L

P

Q

D

D

N

G

G

E

S

V

I

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

P

V

E

E

E

E

E

E

A

G

N

K

D

G

P

K

D

D

-

S

-

S

-

G

-

K

-

K

-

I

-

S

-

A

-

D

-

P

-

A

-

K

-

V

-

E

-

A

F

F

A

R

K

A

E

S

L

L

A

S

S

K

L

L

G

G

E

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

M

E

V

G

G

I

V

T

N

R

-

Y

-

V

L

Q

P

T

Q

A

K

V

A

A

S

G

G

Y

F

L

L

I

M

E

K

K

K

E

E

L

L

M

D

Y

Y

L

F

G

S

K

K

A

A

L

L

Q

E

E

K

P

P

E

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

S
x
A

K
|
K

I

V

S

K

G

D

K
|
K

I

I

D

Q

V

L

L

I

E

K

N

N

L

M

F

L

N

D

K

K

V

V

D

N

T

F

V

M

L

I

I

I

G

G

G

G

A
x
G

M

M

V

A

F

Y

T

T

F

F

F

L

K

K

A

E

-

L

Q

K

G

N

L

M

G

Q

T

I

G

G

N

A

S

S

L
|
L

V

F

E

D

E

E

N

E

K

G

L

A

E

T

L

I

A

V

L

K

S

E

L

I

I

M

E

E

Q

K

A

A

A

E

R

K

K

N

N

G

I

V

K

K

L

I

L

V

L

F

P

P

Q

V

D

D

I

F

I

V

I

T

A

G

P

D

E

K

I

F

S

D

A

E

D

N

A

A

E

K

-

V

S

G

M

Q

A

A

V

T

A

I

V

E

N

S

A

G

I

I

P

P

D

S

G

G

M

W

I

M

G
|
G

V
x
L

D

D

I

C

G

G

P

P

E

E

T

S

R

I

A

K

A

I

Y

N

R

A

Q

Q

E

I

I

V

L

A

G

Q

A

A

R

K

T

L

V

I

L

V

W

W

N

N

G
|
G

P

P

M

I

G
|
G

V
|
V

F

F

E
|
E

I

W

D

D

R

A

F

F

A

A

E

K

G

G

T

T

I

K

A

A

I

L

A

M

E

D

A

E

M

V

A

V

D

K

A

A

T

T

A

S

A

N

G

G

A

C

T

V

T

T

I

I

I

I

G

G

G

G

G
|
G

D
|
D

S

T

A

A

A

T

A

C

V

C

A

A

K

K

A

W

G

G

L

T

A

E

D

D

Q

K

I

V

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

I

L

L

P

P

G

G

I

V

A

E

A

A

L

L

N

S

D

N

active site: R35 (= R37), K212 (= K199), G370 (= G355), G393 (= G378)
binding adenosine-5'-triphosphate: G210 (= G197), A211 (≠ S198), K216 (= K203), G235 (≠ A222), L253 (= L239), G309 (= G294), L310 (≠ V295), G334 (= G319), G337 (= G322), V338 (= V323), E340 (= E325), D371 (= D356)

2wzcA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and aluminium tetrafluoride (see paper)
46% identity, 100% coverage: 2:397/397 of query aligns to 3:404/405 of 2wzcA

query
sites
2wzcA

Q

N

K

K

K

L

T

T

L

L

S

D

D

K

I

L

T

D

I

V

Q

K

G

G

K

K

R

R

V

V

L

V

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

K

E

N

K

N

N

Q

I

I

T

T

D

N

D

N

K

Q

R
|
R

I

I

V

K

E

A

A

A

L

V

P

P

S

S

I

I

K

K

K

F

I

C

I

L

E

D

N

N

G

G

G

A

R

K

-

S

L

V

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

K

V

P

P

N

M

Q

P

D

D

-

K

F

Y

S

S

L

L

T

E

P

P

A

V

A

A

E

V

R

E

L

L

S

K

E

S

L

L

L

L

D

G

C

K

P

D

V

V

I

L

M

F

A

L

G

K

D

D

C

C

I

V

G

G

T

P

E

E

V

V

M

E

Q

K

Q

A

V

C

L

A

A

N

L

P

Q

A

D

A

G

G

E

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

P

V

E

E

E

E

E

E

-

G

-

K

-

G

-

E

-

P

A

A

N

K

D

I

P

E

D

A

F

F

A

R

K

A

E

S

L

L

A

S

S

K

L

L

G

G

E

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

M

E

V

G

G

I

V

T

N

R

-

Y

-

V

L

Q

P

T

Q

A

K

V

A

A

G

G

G

Y

F

L

L

I

M

E

K

K

K

E

E

L

L

M

N

Y

Y

L

F

G

A

K

K

A

A

L

L

Q

E

E

S

P

P

E

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

S
x
A

K
|
K

I

V

S

A

G

D

K
|
K

I

I

D

Q

V

L

L

I

E

N

N

N

L

M

F

L

N

D

K

K

V

V

D

N

T

E

V

M

L

I

I

I

G

G

G
|
G

A
x
G

M

M

V

A

F

F

T

T

F

F

F

L

K

K

A

V

-

L

Q

N

G

N

L

M

G

E

T

I

G

G

N

T

S

S

L

L

V

F

E

D

E

E

N

E

K

G

L

A

E

K

L

I

A

V

L

K

S

D

L

L

I

M

E

S

Q

K

A

A

A

E

R

K

K

N

N

G

I

V

K

K

L

I

L

T

L

L

P

P

Q

V

D

D

I

F

I

V

I

T

A

A

P

D

E

K

I

F

S

D

A

E

D

N

A

A

E

K

S

T

-

G

M

Q

A

A

V

T

A

V

V

A

N

S

A

G

I

I

P

P

D

A

G

G

M

W

I

M

G

G

V

L

D

D

I

C

G

G

P

P

E

E

T

S

R

S

A

K

A

K

Y

Y

R

A

Q

E

E

A

I

V

L

T

G

R

A

A

R

K

T

Q

V

I

L

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

I

W

D

E

R

A

F

F

A

A

E

R

G

G

T

T

I

K

A

A

I

L

A

M

E

D

A

E

M

V

A

V

D

K

A

A

T

T

A

S

A

R

G

G

A

C

T

I

T

T

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

A

A

K

K

A

W

G

N

L

T

A

E

D

D

Q

K

I

V

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

V

A

D

A

A

L

L

N

S

D

N

active site: R37 (= R37), K204 (= K199), G362 (= G355), G385 (= G378)
binding adenosine-5'-diphosphate: G202 (= G197), A203 (≠ S198), K204 (= K199), K208 (= K203), G226 (= G221), G227 (≠ A222), N325 (= N318), P327 (= P320), G329 (= G322), V330 (= V323), E332 (= E325), G361 (= G354), D363 (= D356), T364 (≠ S357)
binding tetrafluoroaluminate ion: R37 (= R37), K204 (= K199), K208 (= K203), G361 (= G354), G362 (= G355), G384 (= G377)

2wzbA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and magnesium trifluoride (see paper)
46% identity, 100% coverage: 2:397/397 of query aligns to 3:404/405 of 2wzbA

query
sites
2wzbA

Q

N

K

K

K

L

T

T

L

L

S

D

D

K

I

L

T

D

I

V

Q

K

G

G

K

K

R

R

V

V

L

V

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

K

E

N

K

N

N

Q

I

I

T

T

D

N

D

N

K

Q

R
|
R

I

I

V

K

E

A

A

A

L

V

P

P

S

S

I

I

K

K

K

F

I

C

I

L

E

D

N

N

G

G

G

A

R

K

-

S

L

V

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

K

V

P

P

N

M

Q

P

D

D

-

K

F

Y

S

S

L

L

T

E

P

P

A

V

A

A

E

V

R

E

L

L

S

K

E

S

L

L

L

L

D

G

C

K

P

D

V

V

I

L

M

F

A

L

G

K

D

D

C

C

I

V

G

G

T

P

E

E

V

V

M

E

Q

K

Q

A

V

C

L

A

A

N

L

P

Q

A

D

A

G

G

E

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

P

V

E

E

E

E

E

E

-

G

-

K

-

G

-

E

-

P

A

A

N

K

D

I

P

E

D

A

F

F

A

R

K

A

E

S

L

L

A

S

S

K

L

L

G

G

E

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

M

E

V

G

G

I

V

T

N

R

-

Y

-

V

L

Q

P

T

Q

A

K

V

A

A

G

G

G

Y

F

L

L

I

M

E

K

K

K

E

E

L

L

M

N

Y

Y

L

F

G

A

K

K

A

A

L

L

Q

E

E

S

P

P

E

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

S
x
A

K
|
K

I

V

S

A

G

D

K
|
K

I

I

D

Q

V

L

L

I

E

N

N

N

L

M

F

L

N

D

K

K

V

V

D

N

T

E

V

M

L

I

I

I

G

G

G
|
G

A
x
G

M

M

V

A

F

F

T

T

F

F

F

L

K

K

A

V

-

L

Q

N

G

N

L

M

G

E

T

I

G

G

N

T

S

S

L

L

V

F

E

D

E

E

N

E

K

G

L

A

E

K

L

I

A

V

L

K

S

D

L

L

I

M

E

S

Q

K

A

A

A

E

R

K

K

N

N

G

I

V

K

K

L

I

L

T

L

L

P

P

Q

V

D

D

I

F

I

V

I

T

A

A

P

D

E

K

I

F

S

D

A

E

D

N

A

A

E

K

S

T

-

G

M

Q

A

A

V

T

A

V

V

A

N

S

A

G

I

I

P

P

D

A

G

G

M

W

I

M

G

G

V

L

D

D

I

C

G

G

P

P

E

E

T

S

R

S

A

K

A

K

Y

Y

R

A

Q

E

E

A

I

V

L

T

G

R

A

A

R

K

T

Q

V

I

L

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

I

W

D

E

R

A

F

F

A

A

E

R

G

G

T

T

I

K

A

A

I

L

A

M

E

D

A

E

M

V

A

V

D

K

A

A

T

T

A

S

A

R

G

G

A

C

T

I

T

T

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

A

A

K

K

A

W

G

N

L

T

A

E

D

D

Q

K

I

V

T

S

H

H

I

V

S

S

T

T

G

G

G
|
G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

V

A

D

A

A

L

L

N

S

D

N

active site: R37 (= R37), K204 (= K199), G362 (= G355), G385 (= G378)
binding adenosine-5'-diphosphate: G202 (= G197), A203 (≠ S198), K204 (= K199), K208 (= K203), G226 (= G221), G227 (≠ A222), N325 (= N318), P327 (= P320), G329 (= G322), V330 (= V323), E332 (= E325), G361 (= G354), D363 (= D356), T364 (≠ S357)
binding trifluoromagnesate: K204 (= K199), K208 (= K203), G361 (= G354), G384 (= G377), G385 (= G378)

1vjcA Structure of pig muscle pgk complexed with mgatp (see paper)
45% identity, 100% coverage: 2:397/397 of query aligns to 4:415/416 of 1vjcA

query
sites
1vjcA

Q

N

K

K

K

L

T

T

L

L

S

D

D

K

I

L

T

D

I

V

Q

K

G

G

K

K

R

R

V

V

L

V

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

K

E

N

K

N

N

Q

I

I

T

T

D

N

D

N

K

Q

R
|
R

I

I

V

K

E

A

A

A

L

I

P

P

S

S

I

I

K

K

K

F

I

C

I

L

E

D

N

N

G

G

G

A

R

K

-

S

L

V

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

K

I

P

P

N

M

Q

P

D

D

-

K

F

Y

S

S

L

L

T

E

P

P

A

V

A

A

E

V

R

E

L

L

S

K

E

S

L

L

L

L

D

G

C

K

P

D

V

V

I

L

M

F

A

L

G

K

D

D

C

C

I

V

G

G

T

P

E

E

V

V

M

E

Q

K

Q

A

V

C

L

A

A

D

L

P

Q

A

D

A

G

G

E

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

P

V

E

E

E

E

E

E

A

G

N

K

D

G

P

K

D

D

-

A

-

S

-

G

-

S

-

K

-

V

-

K

-

A

-

D

-

P

-

A

-

K

-

I

-

E

-

A

F

F

A

R

K

A

E

S

L

L

A

S

S

K

L

L

G

G

E

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

M

E

V

G

G

I

V

T

N

R

-

Y

-

V

L

Q

P

T

K

A

K

V

A

A

G

G

G

Y

F

L

L

I

M

E

K

K

K

E

E

L

L

M

N

Y

Y

L

F

G

A

K

K

A

A

L

L

Q

E

E

S

P

P

E

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

S

A

K
|
K

I

V

S

A

G

D

K
|
K

I

I

D

Q

V

L

L

I

E

N

N

N

L

M

F

L

N

D

K

K

V

V

D

N

T

E

V

M

L

I

I

I

G

G

G

G

A
x
G

M

M

V

A

F

F

T

T

F

F

F

L

K

K

A

V

-

L

Q

N

G

N

L

M

G

E

T

I

G

G

N

T

S

S

L

L

V

F

E

D

E

E

N

E

K

G

L

S

E

K

L

I

A

V

L

K

S

D

L

L

I

M

E

S

Q

K

A

A

A

E

R

K

K

N

N

G

I

V

K

K

L

I

L

T

L

L

P

P

Q

V

D

D

I

F

I

V

I

T

A

A

P

D

E

K

I

F

S

D

A

E

D

N

A

A

E

K

S

T

-

G

M

Q

A

A

V

T

A

V

V

A

N

S

A

G

I

I

P

P

D

A

G

G

M

W

I

M

G

G

V

L

D

D

I

C

G

G

P

P

E

E

T

S

R

S

A

K

A

K

Y

Y

R

S

Q

E

E

A

I

V

L

A

G

R

A

A

R

K

T

Q

V

I

L

V

W

W

N

N

G

G

P

P

M

V

G
|
G

V
|
V

F

F

E
|
E

I

W

D

E

R

A

F

F

A

A

E

Q

G

G

T

T

I

K

A

A

I

L

A

M

E

D

A

E

M

V

A

V

D

K

A

A

T

T

A

S

A

R

G

G

A

C

T

I

T

T

I

I

I

I

G

G

G

G

G
|
G

D
|
D

S

T

A

A

A

T

A

C

V

C

A

A

K

K

A

W

G

N

L

T

A

E

D

D

Q

K

I

V

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

V

A

D

A

A

L

L

N

S

D

N

active site: R38 (= R37), K215 (= K199), G373 (= G355), G396 (= G378)
binding adenosine-5'-triphosphate: G213 (= G197), K219 (= K203), G238 (≠ A222), G340 (= G322), V341 (= V323), E343 (= E325), D374 (= D356)

4axxA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp 3-phosphoglycerate and beryllium trifluoride
45% identity, 100% coverage: 2:397/397 of query aligns to 3:406/407 of 4axxA

query
sites
4axxA

Q

N

K

K

K

L

T

T

L

L

S

D

D

K

I

L

T

D

I

V

Q

K

G

G

K

K

R

R

V

V

L

V

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

K

E

N

K

N

N

Q

I

I

T

T

D

N

D

N

K

Q

R
|
R

I

I

V

K

E

A

A

A

L

V

P

P

S

S

I

I

K

K

K

F

I

C

I

L

E

D

N

N

G

G

G

A

R

K

-

S

L

V

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

K

V

P

P

N

M

Q

P

D

D

-

K

F

Y

S

S

L

L

T

E

P

P

A

V

A

A

E

V

R

E

L

L

S

K

E

S

L

L

L

L

D

G

C

K

P

D

V

V

I

L

M

F

A

L

G

K

D

D

C

C

I

V

G

G

T

P

E

E

V

V

M

E

Q

K

Q

A

V

C

L

A

A

N

L

P

Q

A

D

A

G

G

E

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

P

V

E

E

E

E

E

E

-

G

-

K

-

A

-

K

-

A

-

E

-

P

A

A

N

K

D

I

P

E

D

A

F

F

A

R

K

A

E

S

L

L

A

S

S

K

L

L

G

G

E

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

M

E

V

G

G

I

V

T

N

R

-

Y

-

V

L

Q

P

T

Q

A

K

V

A

A

G

G

G

Y

F

L

L

I

M

E

K

K

K

E

E

L

L

M

N

Y

Y

L

F

G

A

K

K

A

A

L

L

Q

E

E

S

P

P

E

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

S
x
A

K
|
K

I

V

S

A

G

D

K
|
K

I

I

D

Q

V

L

L

I

E

N

N

N

L

M

F

L

N

D

K

K

V

V

D

N

T

E

V

M

L

I

I

I

G

G

G
|
G

A
x
G

M

M

V

A

F

F

T

T

F

F

F

L

K

K

A

V

-

L

Q

N

G

N

L

M

G

E

T

I

G

G

N

T

S

S

L

L

V

F

E

D

E

E

N

E

K

G

L

A

E

K

L

I

A

V

L

K

S

D

L

L

I

M

E

S

Q

K

A

A

A

E

R

K

K

N

N

G

I

V

K

K

L

I

L

T

L

L

P

P

Q

V

D

D

I

F

I

V

I

T

A

A

P

D

E

K

I

F

S

D

A

E

D

N

A

A

E

K

S

T

-

G

M

Q

A

A

V

T

A

V

V

A

N

S

A

G

I

I

P

P

D

A

G

G

M

W

I

M

G

G

V

L

D

D

I

C

G

G

P

P

E

E

T

S

R

S

A

K

A

K

Y

Y

R

A

Q

E

E

A

I

V

L

T

G

R

A

A

R

K

T

Q

V

I

L

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

I

W

D

E

R

A

F

F

A

A

E

R

G

G

T

T

I

K

A

A

I

L

A

M

E

D

A

E

M

V

A

V

D

K

A

A

T

T

A

S

A

R

G

G

A

C

T

I

T

T

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

A

A

K

K

A

W

G

N

L

T

A

E

D

D

Q

K

I

V

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

V

A

D

A

A

L

L

N

S

D

N

active site: R37 (= R37), K206 (= K199), G364 (= G355), G387 (= G378)
binding adenosine-5'-diphosphate: G204 (= G197), A205 (≠ S198), K210 (= K203), G228 (= G221), G229 (≠ A222), N327 (= N318), P329 (= P320), G331 (= G322), V332 (= V323), E334 (= E325), G363 (= G354), G364 (= G355), D365 (= D356), T366 (≠ S357)
binding beryllium trifluoride ion: K206 (= K199), K210 (= K203), G363 (= G354)

1kf0A Crystal structure of pig muscle phosphoglycerate kinase ternary complex with amp-pcp and 3pg (see paper)
44% identity, 100% coverage: 2:397/397 of query aligns to 4:415/416 of 1kf0A

query
sites
1kf0A

Q

N

K

K

K

L

T

T

L

L

S

D

D

K

I

L

T

N

I

V

Q

K

G

G

K

K

R

R

V

V

L

V

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

K

E

N

K

N

N

Q

I

I

T

T

D

N

D

N

K

Q

R
|
R

I

I

V

K

E

A

A

A

L

V

P

P

S

S

I

I

K

K

K

F

I

C

I

L

E

D

N

D

G

G

G

A

R

K

-

S

L

V

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

K

S

P

P

N

M

Q

P

D

D

-

K

F

Y

S

S

L

L

T

Q

P

P

A

V

A

A

E

V

R

E

L

L

S

K

E

S

L

L

L

L

D

G

C

K

P

D

V

V

I

L

M

F

A

L

G

K

D

D

C

C

I

V

G

G

T

P

E

E

V

V

M

E

Q

K

Q

A

V

C

L

A

A

D

L

P

Q

A

D

A

G

G

E

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

P

V

E

E

E

E

E

E

A

G

N

K

D

G

P

K

D

D

-

A

-

S

-

G

-

N

-

K

-

V

-

K

-

A

-

E

-

P

-

A

-

K

-

I

-

K

-

T

F

F

A

R

K

A

E

S

L

L

A

S

S

K

L

L

G

G

E

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

M

E

V

G

G

I

V

T

N

R

-

Y

-

V

L

Q

P

T

K

A

K

V

A

A

G

G

G

Y

F

L

L

I

M

E

K

K

K

E

E

L

L

M

N

Y

Y

L

F

G

A

K

K

A

A

L

L

Q

E

E

S

P

P

E

E

R

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G
|
G

S
x
A

K
|
K

I

V

S

A

G

D

K

K

I

I

D

Q

V

L

L

I

E

N

N

N

L

M

F

L

N

D

K

K

V

V

D

N

T

E

V

M

L

I

I

I

G

G

G
|
G

A
x
G

M

M

V

A

F

F

T

T

F

F

F

L

K

K

A

V

-

L

Q

N

G

N

L

M

G

E

T

I

G

G

N

T

S

S

L

L

V

F

E

D

E

E

N

E

K

G

L

A

E

K

L

I

A

V

L

K

S

N

L

L

I

M

E

S

Q

K

A

A

A

E

R

K

K

N

N

G

I

V

K

K

L

I

L

T

L

L

P

P

Q

V

D

D

I

F

I

V

I

T

A

A

P

D

E

K

I

F

S

D

A

E

D

H

A

A

E

K

S

T

-

G

M

Q

A

A

V

T

A

V

V

A

N

S

A

G

I

I

P

P

D

A

G

G

M

W

I

M

G

G

V

L

D

D

I

C

G

G

P

P

E

E

T

S

R

S

A

K

A

K

Y

Y

R

S

Q

E

E

A

I

V

L

A

G

R

A

A

R

K

T

Q

V

I

L

V

W

W

N

N

G

G

P

P

M

V

G
|
G

V
|
V

F

F

E
|
E

I

W

D

E

R

A

F

F

A

A

E

Q

G

G

T

T

I

K

A

A

I

L

A

M

E

D

A

E

M

V

A

V

D

K

A

A

T

T

A

S

A

R

G

G

A

C

T

I

T

T

I

I

I

I

G

G

G

G

G
|
G

D

D

S

T

A

A

A

T

A

C

V

C

A

A

K

K

A

W

G

N

L

T

A

E

D

D

Q

K

I

V

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

V

L

L

P

P

G

G

I

V

A

D

A

A

L

L

N

S

D

N

active site: R38 (= R37), K215 (= K199), G373 (= G355), G396 (= G378)
binding phosphomethylphosphonic acid adenylate ester: G213 (= G197), A214 (≠ S198), G237 (= G221), G238 (≠ A222), G340 (= G322), V341 (= V323), E343 (= E325)

Query Sequence

>WP_012467397.1 NCBI__GCF_000020465.1:WP_012467397.1
MQKKTLSDITIQGKRVLMRVDFNVPLDEEKNITDDKRIVEALPSIKKIIENGGRLILMSH
LGRPKGKPNQDFSLTPAAERLSELLDCPVIMAGDCIGTEVMQQVLALQDGEVIMLENLRF
HPEEEANDPDFAKELASLGEIYVNDAFGTAHRAHASTEGITRYVQTAVAGYLIEKELMYL
GKALQEPERPFVAILGGSKISGKIDVLENLFNKVDTVLIGGAMVFTFFKAQGLGTGNSLV
EENKLELALSLIEQAARKNIKLLLPQDIIIAPEISADAESMAVAVNAIPDGMIGVDIGPE
TRAAYRQEILGARTVLWNGPMGVFEIDRFAEGTIAIAEAMADATAAGATTIIGGGDSAAA
VAKAGLADQITHISTGGGASLEFLEGKELPGIAALND

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory