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Comparing WP_012468432.1 NCBI__GCF_000020385.1:WP_012468432.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5bntC X-ray crystal structure of a aspartate-semialdehyde dehydrogenase bound to NADP from pseudomonas aeruginosa
68% identity, 100% coverage: 2:366/366 of query aligns to 4:371/371 of 5bntC
- active site: C136 (= C132), Q163 (= Q159), R269 (= R264), H276 (= H271)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G7), R11 (= R9), G12 (= G10), M13 (= M11), V14 (= V12), T38 (= T37), S39 (= S38), Q74 (= Q70)
P44801 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see 2 papers)
66% identity, 99% coverage: 3:364/366 of query aligns to 4:370/371 of P44801
- RGMV 10:13 (= RGMV 9:12) binding NADP(+)
- TS 37:38 (= TS 37:38) binding NADP(+)
- Q74 (= Q70) binding NADP(+)
- R103 (= R99) binding phosphate; mutation to K: 2-fold increase in affinity for ASA, 23-fold decrease in affinity for phosphate, and 275-fold decrease in activity.; mutation to L: 7-fold increase in affinity for ASA, 150-fold decrease in affinity for phosphate, and 1400-fold decrease in activity.
- C136 (= C132) active site, Acyl-thioester intermediate
- S166 (= S162) binding NADP(+)
- E243 (= E238) mutation to D: No change in affinity for ASA and 82-fold decrease in activity.
- K246 (= K241) binding phosphate; mutation to R: 2-fold increase in affinity for ASA, nearly no change in affinity for phosphate, and 30-fold decrease in activity.
- R270 (= R264) mutation to K: 2-fold decrease in affinity for ASA and 825-fold decrease in activity.
- H277 (= H271) active site, Proton acceptor
- Q353 (= Q347) binding NADP(+)
1pquA Crystal structure of the h277n mutant of aspartate semialdehyde dehydrogenase from haemophilus influenzae bound with NADP, s-methyl cysteine sulfoxide and cacodylate (see paper)
66% identity, 99% coverage: 3:364/366 of query aligns to 4:370/371 of 1pquA
- active site: C136 (= C132), Q163 (= Q159), R270 (= R264), N277 (≠ H271)
- binding cysteine: C136 (= C132), Q163 (= Q159), G167 (= G163), E243 (= E238), R270 (= R264)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), R10 (= R9), G11 (= G10), M12 (= M11), V13 (= V12), T36 (= T36), T37 (= T37), S38 (= S38), C73 (= C69), Q74 (= Q70), A99 (= A95), S166 (= S162), L354 (= L348), A358 (= A352)
3pzrA Crystals structure of aspartate beta-semialdehyde dehydrogenase from vibrio cholerae with NADP and product of s-carbamoyl-l-cysteine (see paper)
65% identity, 99% coverage: 1:364/366 of query aligns to 1:367/370 of 3pzrA
- active site: C134 (= C132), Q161 (= Q159), R267 (= R264), H274 (= H271)
- binding cysteine: C134 (= C132), Q161 (= Q159), G165 (= G163), E240 (= E238), R267 (= R264), H274 (= H271), Q350 (= Q347)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G7), R9 (= R9), G10 (= G10), M11 (= M11), V12 (= V12), S35 (≠ T36), T36 (= T37), S37 (= S38)
Q9KQG2 Aspartate-semialdehyde dehydrogenase 1; ASA dehydrogenase 1; ASADH 1; Aspartate-beta-semialdehyde dehydrogenase 1; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
65% identity, 99% coverage: 1:364/366 of query aligns to 1:367/370 of Q9KQG2
- RGMV 9:12 (= RGMV 9:12) binding NADP(+)
- TS 36:37 (= TS 37:38) binding NADP(+)
- Q72 (= Q70) binding NADP(+)
- C134 (= C132) active site, Acyl-thioester intermediate
- SG 164:165 (= SG 162:163) binding NADP(+)
- P192 (≠ A190) binding NADP(+)
- Q350 (= Q347) binding NADP(+)
4r5mA Crystal structure of vc-aspartate beta-semialdehyde-dehydrogenase with NADP and 4-nitro-2-phosphono-benzoic acid (see paper)
65% identity, 99% coverage: 1:364/366 of query aligns to 1:367/369 of 4r5mA
- active site: C134 (= C132), Q161 (= Q159), R267 (= R264), H274 (= H271)
- binding 4-nitro-2-phosphonobenzoic acid: C71 (= C69), G73 (= G71), G74 (= G72), A96 (= A94), A97 (= A95), S98 (= S96), R101 (= R99), K243 (= K241)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G7), R9 (= R9), G10 (= G10), M11 (= M11), V12 (= V12), T36 (= T37), S37 (= S38), Q72 (= Q70), G73 (= G71), G165 (= G163)
1mb4A Crystal structure of aspartate semialdehyde dehydrogenase from vibrio cholerae with NADP and s-methyl-l-cysteine sulfoxide (see paper)
65% identity, 99% coverage: 1:364/366 of query aligns to 1:367/369 of 1mb4A
- active site: C134 (= C132), Q161 (= Q159), R267 (= R264), H274 (= H271)
- binding cysteine: N133 (= N131), C134 (= C132), G165 (= G163), E240 (= E238), R267 (= R264), H274 (= H271)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G7), R9 (= R9), G10 (= G10), M11 (= M11), V12 (= V12), T36 (= T37), S37 (= S38), C71 (= C69), Q72 (= Q70), G73 (= G71), A97 (= A95), S164 (= S162), Q350 (= Q347), L351 (= L348), A355 (= A352)
7tcmA Crystal structure of aspartate-semialdehyde dehydrogenase from acinetobacter baumannii in complex with NADP
64% identity, 100% coverage: 1:365/366 of query aligns to 3:370/373 of 7tcmA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G7), R11 (= R9), G12 (= G10), M13 (= M11), V14 (= V12), T38 (= T37), S39 (= S38), C74 (= C69), Q75 (= Q70), G76 (= G71), A100 (= A95), S167 (= S162), Q352 (= Q347), A357 (= A352)
1tb4A Crystal structure of aspartate-semialdehyde dehydrogenase from haemophilus influenzae with a bound periodate (see paper)
65% identity, 99% coverage: 3:364/366 of query aligns to 4:356/357 of 1tb4A
1ta4A Crystal structure of aspartate-semialdehyde dehydrogenase from haemophilus influenzae with a bound arsenate (see paper)
65% identity, 99% coverage: 3:364/366 of query aligns to 4:356/357 of 1ta4A
1nx6A Crystal structure of aspartate semialdehyde dehydrogenase from haemophilus influenzae as a tetrahedral hemithiocetal reaction intermediate with phosphate at 2.15 a (see paper)
65% identity, 99% coverage: 3:364/366 of query aligns to 4:356/357 of 1nx6A
1gl3A Aspartate beta-semialdehyde dehydrogenase in complex with NADP and substrate analogue s-methyl cysteine sulfoxide (see paper)
64% identity, 99% coverage: 2:363/366 of query aligns to 3:366/367 of 1gl3A
- active site: C135 (= C132), Q162 (= Q159), R267 (= R264), H274 (= H271)
- binding cysteine: C135 (= C132), Q162 (= Q159), I230 (= I227), E241 (= E238), R267 (= R264), H274 (= H271)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R10 (= R9), G11 (= G10), M12 (= M11), V13 (= V12), T37 (= T37), S38 (= S38), C72 (= C69), Q73 (= Q70), A98 (= A95), C135 (= C132), S165 (= S162), G166 (= G163), G168 (= G165), A169 (= A166), Q350 (= Q347), A355 (= A352)
P0A9Q9 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Escherichia coli (strain K12) (see 2 papers)
64% identity, 99% coverage: 2:363/366 of query aligns to 3:366/367 of P0A9Q9
- RGMV 10:13 (= RGMV 9:12) binding NADP(+)
- TS 37:38 (= TS 37:38) binding NADP(+)
- Q73 (= Q70) binding NADP(+)
- C135 (= C132) active site, Acyl-thioester intermediate; mutation to A: Complete loss of activity.; mutation to S: 99.7% loss of activity.
- SGGGA 165:169 (≠ SGAGA 162:166) binding NADP(+)
- R173 (= R170) binding NADP(+)
- P193 (≠ A190) binding NADP(+)
- Q350 (= Q347) binding NADP(+)
1pqpA Crystal structure of the c136s mutant of aspartate semialdehyde dehydrogenase from haemophilus influenzae bound with aspartate semialdehyde and phosphate (see paper)
65% identity, 99% coverage: 3:364/366 of query aligns to 4:356/357 of 1pqpA
- active site: S122 (≠ C132), Q149 (= Q159), R256 (= R264), H263 (= H271)
- binding l-homoserine: S122 (≠ C132), Q149 (= Q159), G153 (= G163), R256 (= R264), H263 (= H271), Q339 (= Q347)
- binding phosphate ion: R89 (= R99), N121 (= N131), S122 (≠ C132), K232 (= K241)
8jusA Crystal structure of aspartate semialdehyde dehydrogenase from porphyromonas gingivalis complexed with 2',5'adenosine diphosphate
27% identity, 96% coverage: 1:353/366 of query aligns to 1:319/335 of 8jusA
Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
28% identity, 96% coverage: 1:353/366 of query aligns to 5:330/346 of Q04797
- S98 (= S96) modified: Phosphoserine
- Y146 (vs. gap) modified: Phosphotyrosine
3pylC Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with d-2,3-diaminopropionate (see paper)
25% identity, 96% coverage: 3:353/366 of query aligns to 4:330/361 of 3pylC
3q11A Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with NADP and aspartyl beta- difluorophosphonate (see paper)
25% identity, 96% coverage: 3:353/366 of query aligns to 4:330/358 of 3q11A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), T10 (≠ R9), G11 (= G10), A35 (≠ T37), S36 (= S38), R38 (≠ P45), S39 (≠ M46), T56 (vs. gap), A71 (≠ Q70), T75 (≠ Y74), G160 (= G165), M161 (= M176), G162 (= G177)
- binding 5,5-difluoro-4-oxo-5-phosphono-D-norvaline: R98 (= R99), N126 (= N131), C127 (= C132), Q154 (= Q159), G158 (= G163), E219 (= E238), K222 (= K241), R244 (= R264)
4r51A Crystal complex structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide adenine dinucleotide phosphate and phthalic acid (see paper)
25% identity, 96% coverage: 3:353/366 of query aligns to 4:330/360 of 4r51A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), T10 (≠ R9), G11 (= G10), A12 (≠ M11), V13 (= V12), A35 (≠ T37), S36 (= S38), S39 (≠ M46), T56 (vs. gap), S70 (≠ C69), A71 (≠ Q70), G72 (= G71), N93 (≠ A94), T94 (≠ A95), N126 (= N131), C127 (= C132), G160 (= G165), M161 (= M176), G328 (= G351)
- binding phthalic acid: S73 (≠ G72), T94 (≠ A95), S95 (= S96), R98 (= R99), N126 (= N131), K222 (= K241)
4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
25% identity, 96% coverage: 3:353/366 of query aligns to 4:330/359 of 4r5hA
- binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ G72), T94 (≠ A95), S95 (= S96), R98 (= R99), N126 (= N131), C127 (= C132), Q154 (= Q159), G158 (= G163), K222 (= K241), R244 (= R264), H251 (= H271)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), T10 (≠ R9), G11 (= G10), A12 (≠ M11), V13 (= V12), A35 (≠ T37), S36 (= S38), R38 (≠ P45), S39 (≠ M46), T56 (vs. gap), S70 (≠ C69), A71 (≠ Q70), G72 (= G71), T75 (≠ Y74), N93 (≠ A94), T94 (≠ A95), P125 (≠ G130), N126 (= N131), C127 (= C132), G160 (= G165), M161 (= M176), G328 (= G351)
Query Sequence
>WP_012468432.1 NCBI__GCF_000020385.1:WP_012468432.1
MKVGIVGWRGMVGSVLMQRMQEENDFALGFEPVFFTTSQAGQPAPMGAGTLKKADDIEEL
KKLDVIITCQGGDYTKAVHPELRKQGWNGYWIDAASTLRMEDNAVIILDPVNRNVIDKAL
ANGQKDFIGGNCTVSLMLMGLGGLFRAGLVEWISSMTYQAASGAGAPNMRELLSQMGVLQ
GVVAKELKDAGSAILEIDKKVTQTLRDGSMPTKEFGFPLAGNVLPWIDREVEDGQSREEL
KGFQETNKILGTSAPIPVDGICVRVGAMRCHSQAITIKLNKDLPLAEIEQLIANDNQWVK
LIPNNKADTLAGLTPAAVSGTLTVPVGRVRKMKMGPQYVQAFTCGDQLLWGAAEPLRRML
RILLEK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory