SitesBLAST

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
30% identity, 94% coverage: 4:567/598 of query aligns to 92:647/664 of P09114

query
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P09114

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P191 (= P114) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (≠ T492) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
29% identity, 94% coverage: 4:567/598 of query aligns to 95:650/667 of P09342

query
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P09342

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C161 (≠ V70) modified: Disulfide link with 307
P194 (= P114) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ L226) modified: Disulfide link with 161

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 12:554/582 of 3ea4A

query
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E

A

A

L

F

F

F

L

L

A

A

G

T

H

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

L

V

D

D

I

V

P

P

L
x
K

N

D

V

I

Q

Q

G

Q

A

Q

L

L

I

A

E

I

T

P

D

N

A

W

L

E

Q

Q

H

A

Y

M

D

R

P

L

S

P

E

G

D

Y

A

M

V

S

Q

R

V

M

V

P

A

K

P

P

S

E

N

D

D

S

V

H

Y

L

S

E

Q

Q

L

I

I

V

E

R

K

L

I

I

C

S

N

E

A

S

R

K

K

K

P

P

V

V

I

L

L

Y

A

V

G
|
G

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x
G

G
|
G

I

C

R

L

L

-

S

-

G

N

S

S

H

S

D

D

V

E

F

L

I

G

Q

R

L

F

L

V

E

E

K

L

L

T

N

G

I

I

P

P

V

V

V

A

T

T

A
x
T

W
x
L

N
x
M

A

G

H

L

D

G

N

S

L

Y

W

P

D

C

D

D

H

D

P

E

L

L

Y

S

C

L

G

H

R

M

P
x
L

G
|
G

T
x
M

I
x
H

G

G

T

T

R

V

G

Y

G

A

N

N

F

Y

V

A

V

V

Q

E

N

H

C

S

D

D

L

L

S

A

L

F

G
|
G

C

V

R
|
R

M

F

N
x
D

I
x
D

R
|
R

Q

-

I
x
V

S

T

Y

G

N

K

W

L

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E

N

A

F

F

A

A

K

S

D

R

A

A

Y

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L

I

V

V

A

H

V

I

D
|
D

I
|
I

D

D

A

S

G

A

E

E

L

I

K

G

K

K

P

-

T

N

L

K

T

T

V

P

D

H

M

V

P

S

I

V

H

C

A
x
G

D
|
D

L
x
V

G

K

D

L

F

A

L

L

P

Q

G

G

L

M

L

N

G

K

A

V

M

L

Q

E

A

N

D

R

H

A

K

E

L

E

G

L

N

K

I

L

E

D

W

F

L

G

E

V

W

W

C

R

R

N

K

E

I

L

N

N

-

V

-

Q

-

K

Q

Q

K

K

Y

F

P

P

A

L

V

-

Q

-

P

-

H

S

H

F

Y

K

T

T

P

F

H

G

S

E

P

A

V

I

N

P

P

P

Y

Q

V

Y

F

A

M

I

D

K

R

V

L

L

S

D

Q

E

Y

L

L

T

D

D

E

G

G

K

D

A

V

I

T

I

V

S

T

T

G

G

N
x
V

G
|
G

S
x
Q

A
x
H

C

Q

V
x
M

C

W

S

A

F

A

Q

Q

A

F

M

Y

V

N

I

Y

K

K

N

P

Q

R

R

Q

L

W

F

L

T

S

N

S

S
x
G

G

G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

L

I

G

G

A

A

A

S

V

V

A

A

L

N

G

P

G

D

K

A

R

I

V

V

I

V

C

D

L

I

D

D

G
|
G

D
|
D

G
|
G

S
|
S

I

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

N

E

K

N

L

L

N

P

L

V

K

K

I

V

F

L

W

L

L

L

N

N

N
|
N

D

Q

G
x
H

Y
x
L

H
x
G

S
x
M

I
x
V

R

M

Q

Q

T
x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

T

Q

S

E

G

D

I

E

S

I

F

F

P

P

S

N

A

M

D

L

K

L

I

F

A

A

R

A

A

A

Y

C

G

G

F

I

E

P

F

A

I

A

K

R

I

V

D

T

A

K

V

K

E

A

D

D

M

L

D

R

E

E

K

A

I

I

K

Q

K

T

V

M

L

L

L

D

S

T

D

P

T

G

S

P

I

Y

V

L

C

L

E

D

V

V

I

I

active site: Y32 (≠ V24), G34 (= G26), G35 (= G27), A36 (≠ G28), S37 (≠ A29), E58 (= E50), T81 (= T73), F120 (vs. gap), Q121 (= Q121), E122 (= E122), K170 (≠ L170), M265 (≠ T267), V292 (≠ I295), V399 (≠ N403), G425 (≠ A429), M427 (= M431), D452 (= D456), N479 (= N483), H481 (≠ G485), L482 (≠ Y486), M484 (≠ S488), V485 (≠ I489), W488 (≠ T492)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (≠ I292), R291 (= R293), W488 (≠ T492)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R160), G221 (= G221), G222 (≠ S222), G223 (= G223), T245 (≠ A247), L246 (≠ W248), M247 (≠ N249), L263 (≠ P265), G264 (= G266), M265 (≠ T267), H266 (≠ I268), G285 (= G287), R287 (= R289), D289 (≠ N291), R291 (= R293), D309 (= D312), I310 (= I313), G327 (≠ A331), D328 (= D332), V329 (≠ L333), M404 (≠ V408), G422 (≠ S426)
binding magnesium ion: D452 (= D456), N479 (= N483), H481 (≠ G485)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ N403), G400 (= G404), Q401 (≠ S405), H402 (≠ A406), M427 (= M431), G451 (= G455), D452 (= D456), G453 (= G457), S454 (= S458), N479 (= N483), H481 (≠ G485), L482 (≠ Y486), G483 (≠ H487), M484 (≠ S488), V485 (≠ I489)

Sites not aligning to the query:

active site: 557
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: 567

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 12:554/582 of 3e9yA

query
sites
3e9yA

K

K

V

G

S

A

D

D

Y

I

I

L

A

V

S

E

F

A

M

L

V

E

E

R

N

Q

G

G

I

V

T

E

D

T

L

V

F

F

T

A

V
x
Y

T

P

G
|
G

G
x
A

A
x
S

M

M

H

E

L

I

N

H

D

Q

S

A

L

L

G

T

N

R

H

S

P

S

E

S

L

I

V

R

S

N

T

V

Y

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

A

G

Y

Y

A

A

R

R

L

S

T

S

G

G

R

K

I

P

A

G

L

I

V

C

C

I

V

A

T
|
T

S

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

M

A

G

D

G

A

W

L

V

L

D

D

S

S

I

V

P

P

M

L

F

V

V

A

I

I

S

T

G

G

Q

Q

V

-

K

-

F

-

S

-

T

-

I

-

A

-

S

-

T

-

N

-

V

V

P

P

L

R

R

R

Q

M

L

I

G

G

D

T

-

D

-

A

-
x
F

Q
|
Q

E
|
E

F

T

N

P

I

I

V

V

D

E

S

V

V

T

R

R

C

S

M

I

T

T

K

K

Y

H

A

N

V

Y

M

L

L

V

T

M

E

D

P

V

E

E

S

D

I

I

A

P

Y

R

H

I

L

I

E

E

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E

A

A

L

F

F

F

L

L

A

A

G

T

H

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

L

V

D

D

I

V

P

P

L
x
K

N

D

V

I

Q

Q

G

Q

A

Q

L

L

I

A

E

I

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D

N

A

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Y

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Y

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S

E

Q

Q

L

I

I

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E

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K

L

I

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E

A

S

R

K

K

K

P

P

V

V

I

L

L

Y

A

V

G
|
G

S
x
G

G
|
G

I

C

R

L

L

-

S

-

G

N

S

S

H

S

D

D

V

E

F

L

I

G

Q

R

L

F

L

V

E

E

K

L

L

T

N

G

I

I

P

P

V

V

V

A

T

T

A
x
T

W
x
L

N
x
M

A

G

H

L

D

G

N

S

L

Y

W

P

D

C

D

D

H

D

P

E

L

L

Y

S

C

L

G

H

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M

P
x
L

G

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T
x
M

I

H

G

G

T

T

R

V

G

Y

G

A

N

N

F

Y

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A

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H

C

S

D

D

L

L

S

A

L

F

G
|
G

C

V

R
|
R

M

F

N
x
D

I
x
D

R
|
R

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-

I
x
V

S

T

Y

G

N

K

W

L

E

E

N

A

F

F

A

A

K

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A

A

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K

L

I

V

V

A

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V

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D
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D

I
|
I

D

D

A

S

G

A

E

E

L

I

K

G

K

K

P

-

T

N

L

K

T

T

V

P

D

H

M

V

P

S

I

V

H

C

A
x
G

D
|
D

L
x
V

G

K

D

L

F

A

L

L

P

Q

G

G

L

M

L

N

G

K

A

V

M

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Q

E

A

N

D

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H

A

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F

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W

C

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R

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I

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-

V

-

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-

K

Q

Q

K

K

Y

F

P

P

A

L

V

-

Q

-

P

-

H

S

H

F

Y

K

T

T

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F

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E

P

A

V

I

N

P

P

P

Y

Q

V

Y

F

A

M

I

D

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Q

E

Y

L

L

T

D

D

E

G

G

K

D

A

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I

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I

V

S

T

T

G

G

N
x
V

G
|
G

S
x
Q

A
x
H

C

Q

V
x
M

C

W

S

A

F

A

Q

Q

A

F

M

Y

V

N

I

Y

K

K

N

P

Q

R

R

Q

L

W

F

L

T

S

N

S

S
x
G

G

G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

L

I

G

G

A

A

A

S

V

V

A

A

L

N

G

P

G

D

K

A

R

I

V

V

I

V

C

D

L

I

D

D

G
|
G

D
|
D

G
|
G

S
|
S

I

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

N

E

K

N

L

L

N

P

L

V

K

K

I

V

F

L

W

L

L

L

N

N

N
|
N

D

Q

G
x
H

Y
x
L

H
x
G

S
x
M

I
x
V

R

M

Q

Q

T
x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

T

Q

S

E

G

D

I

E

S

I

F

F

P

P

S

N

A

M

D

L

K

L

I

F

A

A

R

A

A

A

Y

C

G

G

F

I

E

P

F

A

I

A

K

R

I

V

D

T

A

K

V

K

E

A

D

D

M

L

D

R

E

E

K

A

I

I

K

Q

K

T

V

M

L

L

L

D

S

T

D

P

T

G

S

P

I

Y

V

L

C

L

E

D

V

V

I

I

active site: Y32 (≠ V24), G34 (= G26), G35 (= G27), A36 (≠ G28), S37 (≠ A29), E58 (= E50), T81 (= T73), F120 (vs. gap), Q121 (= Q121), E122 (= E122), K170 (≠ L170), M265 (≠ T267), V292 (≠ I295), V399 (≠ N403), G425 (≠ A429), M427 (= M431), D452 (= D456), N479 (= N483), H481 (≠ G485), L482 (≠ Y486), M484 (≠ S488), V485 (≠ I489), W488 (≠ T492)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (≠ I292), R291 (= R293), W488 (≠ T492)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R160), G221 (= G221), G222 (≠ S222), G223 (= G223), T245 (≠ A247), L246 (≠ W248), M247 (≠ N249), L263 (≠ P265), G285 (= G287), R287 (= R289), D289 (≠ N291), R291 (= R293), D309 (= D312), I310 (= I313), G327 (≠ A331), D328 (= D332), V329 (≠ L333), M404 (≠ V408), G422 (≠ S426)
binding magnesium ion: D452 (= D456), N479 (= N483), H481 (≠ G485)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ N403), G400 (= G404), Q401 (≠ S405), H402 (≠ A406), M427 (= M431), G451 (= G455), G453 (= G457), S454 (= S458), N479 (= N483), H481 (≠ G485), L482 (≠ Y486), G483 (≠ H487), M484 (≠ S488), V485 (≠ I489)

Sites not aligning to the query:

active site: 557
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: 567

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 13:555/582 of 8et4A

query
sites
8et4A

K

K

V

G

S

A

D

D

Y

I

I

L

A

V

S

E

F

A

M

L

V

E

E

R

N

Q

G

G

I

V

T

E

D

T

L

V

F

F

T

A

V

Y

T

P

G

G

G

A

A

S

M

M

H

E

L

I

N

H

D

Q

S

A

L

L

G

T

N

R

H

S

P

S

E

S

L

I

V

R

S

N

T

V

Y

L

N

P

H

R

H

H

E

E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

A

G

Y

Y

A

A

R

R

L

S

T

S

G

G

R

K

I

P

A

G

L

I

V

C

C

I

V

A

T

T

S

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

M

A

G

D

G

A

W

L

V

L

D

D

S

S

I

V

P

P

M

L

F

V

V

A

I

I

S

T

G

G

Q

Q

V

-

K

-

F

-

S

-

T

-

I

-

A

-

S

-

T

-

N

-

V

V

P

P

L

R

R

R

Q

M

L

I

G

G

D

T

-

D

-

A

-

F

Q

Q

E

E

F

T

N

P

I

I

V

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K

N

L

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K

I

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F

L

W

L

L

L

N

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N

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x
H

Y
x
L

H
x
G

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x
M

I
x
V

R

M

Q

Q

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x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

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N

P

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A

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N

A

M

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A

A

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A

A

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Y

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G

G

F

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F

A

I

A

K

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I

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x
K

E

A

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ S405), H403 (≠ A406), G426 (≠ A429), M428 (= M431), G452 (= G455), D453 (= D456), G454 (= G457), S455 (= S458), M458 (= M461), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ H487), M485 (≠ S488), V486 (≠ I489)
binding flavin-adenine dinucleotide: R161 (= R160), G222 (= G221), G223 (≠ S222), G224 (= G223), T246 (≠ A247), L247 (≠ W248), M248 (≠ N249), L264 (≠ P265), M266 (≠ T267), H267 (≠ I268), G286 (= G287), V287 (≠ C288), R288 (= R289), D290 (≠ N291), R292 (= R293), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ L333), M405 (≠ V408), G423 (≠ S426)
binding magnesium ion: F370 (≠ H373), D453 (= D456), M458 (= M461), Q461 (= Q464), N480 (= N483), H482 (≠ G485), K533 (≠ V532)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (≠ T267), R292 (= R293), M485 (≠ S488), W489 (≠ T492)

Sites not aligning to the query:

binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: 568

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 13:555/582 of 5wj1A

query
sites
5wj1A

K

K

V

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M

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N

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I

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N

E

K

N

L

L

N

P

L

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K

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F

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W

L

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L

N

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|
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D

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x
H

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x
L

H
x
G

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x
M

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x
V

R

M

Q

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x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

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Q

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E

G

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N

A

M

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K

L

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A

A

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A

A

A

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C

G

G

F

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P

F

A

I

A

K

R

I

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D

T

A

K

V

K

E

A

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D

M

L

D

R

E

E

K

A

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I

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K

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M

L

L

L

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I

active site: Y33 (≠ V24), G35 (= G26), G36 (= G27), A37 (≠ G28), S38 (≠ A29), E59 (= E50), T82 (= T73), F121 (vs. gap), Q122 (= Q121), E123 (= E122), K171 (≠ L170), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (= D456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R160), G222 (= G221), G223 (≠ S222), G224 (= G223), T246 (≠ A247), L247 (≠ W248), M248 (≠ N249), M263 (≠ R264), L264 (≠ P265), G286 (= G287), R288 (= R289), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ L333), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (= D456), N480 (= N483), H482 (≠ G485)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (≠ T267), D291 (≠ I292), R292 (= R293), M485 (≠ S488), W489 (≠ T492)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ S405), H403 (≠ A406), M428 (= M431), D453 (= D456), G454 (= G457), S455 (= S458), M458 (= M461), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ H487), M485 (≠ S488), V486 (≠ I489)

Sites not aligning to the query:

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 13:555/582 of 5k6tA

query
sites
5k6tA

K

K

V

G

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A

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D

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I

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G

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x
Y

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G
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G

G
x
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A
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M

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H

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E
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E

Q

Q

A

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C

G

A

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I

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A

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E

A

G

Y

Y

A

A

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S

G

G

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A

G

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V

C

C

I

V

A

T
|
T

S

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

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T

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G

G

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A

G

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G

A

W

L

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L

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-

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R

Q

M

L

I

G

G

D

T

-

D

-

A

-
x
F

Q
|
Q

E
|
E

F

T

N

P

I

I

V

V

D

E

S

V

V

T

R

R

C

S

M

I

T

T

K

K

Y

H

A

N

V

Y

M

L

L

V

T

M

E

D

P

V

E

E

S

D

I

I

A

P

Y

R

H

I

L

I

E

E

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E

A

A

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F

F

F

L

L

A

A

G

T

H

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

L

V

D

D

I

V

P

P

L
x
K

N

D

V

I

Q

Q

G

Q

A

Q

L

L

I

A

E

I

T

P

D

N

A

W

L

E

Q

Q

H

A

Y

M

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P

L

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P

E

G

D

Y

A

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Q

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A

K

P

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N

D

D

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Y

L

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E

Q

Q

L

I

I

V

E

R

K

L

I

I

C

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E

A

S

R

K

K

K

P

P

V

V

I

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L

Y

A

V

G
|
G

S
x
G

G
|
G

I

C

R

L

L

-

S

-

G

N

S

S

H

S

D

D

V

E

F

L

I

G

Q

R

L

F

L

V

E

E

K

L

L

T

N

G

I

I

P

P

V

V

V

A

T

S

A
x
T

W
x
L

N
x
M

A

G

H

L

D

G

N

S

L

Y

W

P

D

C

D

D

H

D

P

E

L

L

Y

S

C

L

G

H

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M

P
x
L

G

G

T
x
M

I
x
H

G

G

T

T

R

V

G

Y

G

A

N

N

F

Y

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A

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Q

E

N

H

C

S

D

D

L

L

S

A

L

F

G
|
G

C

V

R
|
R

M

F

N
x
D

I

D

R
|
R

Q

-

I
x
V

S

T

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G

N

K

W

L

E

E

N

A

F

F

A

A

K

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D

R

A

A

Y

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L

I

V

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A

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I

D
|
D

I
|
I

D

D

A

S

G

A

E

E

L

I

K

G

K

K

P

-

T

N

L

K

T

T

V

P

D

H

M

V

P

S

I

V

H

C

A

G

D
|
D

L
x
V

G

K

D

L

F

A

L

L

P

Q

G

G

L

M

L

N

G

K

A

V

M

L

Q

E

A

N

D

R

H

A

K

E

L

E

G

L

N

K

I

L

E

D

W

F

L

G

E

V

W

W

C

R

R

N

K

E

I

L

N

N

-

V

-

Q

-

K

Q

Q

K

K

Y

F

P

P

A

L

V

-

Q

-

P

-

H

S

H

F

Y

K

T

T

P

F

H

G

S

E

P

A

V

I

N

P

P

P

Y

Q

V

Y

F

A

M

I

D

K

R

V

L

L

S

D

Q

E

Y

L

L

T

D

D

E

G

G

K

D

A

V

I

T

I

V

S

T

T

G

G

N
x
V

G
|
G

S
x
Q

A
x
H

C
x
Q

V
x
M

C

W

S

A

F

A

Q

Q

A

F

M

Y

V

N

I

Y

K

K

N

P

Q

R

R

Q

L

W

F

L

T

S

N

S

S
x
G

G

G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

L

I

G

G

A

A

A

S

V

V

A

A

L

N

G

P

G

D

K

A

R

I

V

V

I

V

C

D

L

I

D

D

G
|
G

D
|
D

G
|
G

S
|
S

I

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

N

E

K

N

L

L

N

P

L

V

K

K

I

V

F

L

W

L

L

L

N

N

N
|
N

D

Q

G
x
H

Y
x
L

H
x
G

S
x
M

I
x
V

R

M

Q

Q

T
x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

T

Q

S

E

G

D

I

E

S

I

F

F

P

P

S

N

A

M

D

L

K

L

I

F

A

A

R

A

A

A

Y

C

G

G

F

I

E

P

F

A

I

A

K

R

I

V

D

T

A

K

V

K

E

A

D

D

M

L

D

R

E

E

K

A

I

I

K

Q

K

T

V

M

L

L

L

D

S

T

D

P

T

G

S

P

I

Y

V

L

C

L

E

D

V

V

I

I

active site: Y33 (≠ V24), G35 (= G26), G36 (= G27), A37 (≠ G28), S38 (≠ A29), E59 (= E50), T82 (= T73), F121 (vs. gap), Q122 (= Q121), E123 (= E122), K171 (≠ L170), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (= D456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (≠ I268), R292 (= R293), M485 (≠ S488), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R160), G222 (= G221), G223 (≠ S222), G224 (= G223), T246 (≠ A247), L247 (≠ W248), M248 (≠ N249), L264 (≠ P265), G286 (= G287), R288 (= R289), D290 (≠ N291), R292 (= R293), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ L333), Q404 (≠ C407), M405 (≠ V408), G423 (≠ S426)
binding magnesium ion: D453 (= D456), N480 (= N483), H482 (≠ G485)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ S405), H403 (≠ A406), G426 (≠ A429), M428 (= M431), G452 (= G455), G454 (= G457), S455 (= S458), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ H487)

Sites not aligning to the query:

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 13:555/582 of 5k6rA

query
sites
5k6rA

K

K

V

G

S

A

D

D

Y

I

I

L

A

V

S

E

F

A

M

L

V

E

E

R

N

Q

G

G

I

V

T

E

D

T

L

V

F

F

T

A

V
x
Y

T

P

G
|
G

G
x
A

A
x
S

M

M

H

E

L

I

N

H

D

Q

S

A

L

L

G

T

N

R

H

S

P

S

E

S

L

I

V

R

S

N

T

V

Y

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

A

G

Y

Y

A

A

R

R

L

S

T

S

G

G

R

K

I

P

A

G

L

I

V

C

C

I

V

A

T
|
T

S

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

M

A

G

D

G

A

W

L

V

L

D

D

S

S

I

V

P

P

M

L

F

V

V

A

I

I

S

T

G

G

Q

Q

V

-

K

-

F

-

S

-

T

-

I

-

A

-

S

-

T

-

N

-

V

V

P

P

L

R

R

R

Q

M

L

I

G

G

D

T

-

D

-

A

-
x
F

Q
|
Q

E
|
E

F

T

N

P

I

I

V

V

D

E

S

V

V

T

R

R

C

S

M

I

T

T

K

K

Y

H

A

N

V

Y

M

L

L

V

T

M

E

D

P

V

E

E

S

D

I

I

A

P

Y

R

H

I

L

I

E

E

R

E

A

A

L

F

F

F

L

L

A

A

G

T

H

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

L

V

D

D

I

V

P

P

L
x
K

N

D

V

I

Q

Q

G

Q

A

Q

L

L

I

A

E

I

T

P

D

N

A

W

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Q

H

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E

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D

Y

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E

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L

I

I

V

E

R

K

L

I

I

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E

A

S

R

K

K

K

P

P

V

V

I

L

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Y

A

V

G
|
G

S
x
G

G
|
G

I

C

R

L

L

-

S

-

G

N

S

S

H

S

D

D

V

E

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I

G

Q

R

L

F

L

V

E

E

K

L

L

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N

G

I

I

P

P

V

V

V

A

T

S

A
x
T

W
x
L

N
x
M

A

G

H

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D

G

N

S

L

Y

W

P

D

C

D

D

H

D

P

E

L

L

Y

S

C

L

G

H

R

M

P
x
L

G

G

T
x
M

I

H

G

G

T

T

R

V

G

Y

G

A

N

N

F

Y

V

A

V

V

Q

E

N

H

C

S

D

D

L

L

S

A

L

F

G
|
G

C

V

R
|
R

M

F

N

D

I

D

R
|
R

Q

-

I
x
V

S

T

Y

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N

K

W

L

E

E

N

A

F

F

A

A

K

S

D

R

A

A

Y

K

L

I

V

V

A

H

V

I

D
|
D

I
|
I

D

D

A

S

G

A

E

E

L

I

K

G

K

K

P

-

T

N

L

K

T

T

V

P

D

H

M

V

P

S

I

V

H

C

A
x
G

D
|
D

L
x
V

G

K

D

L

F

A

L

L

P

Q

G

G

L

M

L

N

G

K

A

V

M

L

Q

E

A

N

D

R

H

A

K

E

L

E

G

L

N

K

I

L

E

D

W

F

L

G

E

V

W

W

C

R

R

N

K

E

I

L

N

N

-

V

-

Q

-

K

Q

Q

K

K

Y

F

P

P

A

L

V

-

Q

-

P

-

H

S

H

F

Y

K

T

T

P

F

H

G

S

E

P

A

V

I

N

P

P

P

Y

Q

V

Y

F

A

M

I

D

K

R

V

L

L

S

D

Q

E

Y

L

L

T

D

D

E

G

G

K

D

A

V

I

T

I

V

S

T

T

G

G

N
x
V

G
|
G

S
x
Q

A
x
H

C

Q

V
x
M

C

W

S

A

F

A

Q

Q

A

F

M

Y

V

N

I

Y

K

K

N

P

Q

R

R

Q

L

W

F

L

T

S

N

S

S
x
G

G

G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

L

I

G

G

A

A

A

S

V

V

A

A

L

N

G

P

G

D

K

A

R

I

V

V

I

V

C

D

L

I

D

D

G

G

D
|
D

G
|
G

S
|
S

I

F

Q

I

M
|
M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

N

E

K

N

L

L

N

P

L

V

K

K

I

V

F

L

W

L

L

L

N

N

N
|
N

D

Q

G
x
H

Y
x
L

H
x
G

S
x
M

I
x
V

R

M

Q

Q

T
x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

T

Q

S

E

G

D

I

E

S

I

F

F

P

P

S

N

A

M

D

L

K

L

I

F

A

A

R

A

A

A

Y

C

G

G

F

I

E

P

F

A

I

A

K

R

I

V

D

T

A

K

V

K

E

A

D

D

M

L

D

R

E

E

K

A

I

I

K

Q

K

T

V

M

L

L

L

D

S

T

D

P

T

G

S

P

I

Y

V

L

C

L

E

D

V

V

I

I

active site: Y33 (≠ V24), G35 (= G26), G36 (= G27), A37 (≠ G28), S38 (≠ A29), E59 (= E50), T82 (= T73), F121 (vs. gap), Q122 (= Q121), E123 (= E122), K171 (≠ L170), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (= D456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R293), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R160), G222 (= G221), G223 (≠ S222), G224 (= G223), T246 (≠ A247), L247 (≠ W248), M248 (≠ N249), L264 (≠ P265), M266 (≠ T267), G286 (= G287), R288 (= R289), R292 (= R293), V293 (≠ I295), D310 (= D312), I311 (= I313), G328 (≠ A331), D329 (= D332), V330 (≠ L333), M405 (≠ V408), G423 (≠ S426)
binding magnesium ion: D453 (= D456), N480 (= N483), H482 (≠ G485)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ S405), H403 (≠ A406), G426 (≠ A429), M428 (= M431), D453 (= D456), G454 (= G457), S455 (= S458), M458 (= M461), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ H487), M485 (≠ S488), V486 (≠ I489)

Sites not aligning to the query:

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 13:555/582 of 1z8nA

query
sites
1z8nA

K

K

V

G

S

A

D

D

Y

I

I

L

A

V

S

E

F

A

M

L

V

E

E

R

N

Q

G

G

I

V

T

E

D

T

L

V

F

F

T

A

V
x
Y

T

P

G
|
G

G
x
A

A
x
S

M

M

H

E

L

I

N

H

D

Q

S

A

L

L

G

T

N

R

H

S

P

S

E

S

L

I

V

R

S

N

T

V

Y

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

A

G

Y

Y

A

A

R

R

L

S

T

S

G

G

R

K

I

P

A

G

L

I

V

C

C

I

V

A

T
|
T

S

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

M

A

G

D

G

A

W

L

V

L

D

D

S

S

I

V

P

P

M

L

F

V

V

A

I

I

S

T

G

G

Q

Q

V

-

K

-

F

-

S

-

T

-

I

-

A

-

S

-

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-

N

-

V

V

P

P

L

R

R

R

Q

M

L

I

G

G

D

T

-

D

-

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-
x
F

Q
|
Q

E
|
E

F

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N

P

I

I

V

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E

S

V

V

T

R

R

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K

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I

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A

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R

P

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G

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I

V

P

P

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x
K

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A

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I

A

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I

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D

N

A

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x
Y

A

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x
R

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A

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N

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Y

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L

I

I

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E

R

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L

I

I

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E

A

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A

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G
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G

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G

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-

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D

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E

K

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N

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I

I

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A
x
T

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x
L

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x
M

A

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D

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x
L

G
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G

T
x
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I
x
H

G

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R

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G

Y

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A

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Y

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D

L

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x
V

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R

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x
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I
x
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R

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x
V

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N

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W

L

E

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N

A

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A

A

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A

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D

I
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I

D
|
D

A

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G

A

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E

L

I

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G

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K

P

-

T

N

L

K

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T

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P

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H

M

V

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S

I

V

H

C

A

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D
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D

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x
V

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G

G

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A

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E

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E

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I

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E

D

W

F

L

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E

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W

W

C

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R

N

K

E

I

L

N

N

-

V

-

Q

-

K

Q

Q

K

K

Y

F

P

P

A

L

V

-

Q

-

P

-

H

S

H

F

Y

K

T

T

P

F

H

G

S

E

P

A

V

I

N

P

P

P

Y

Q

V

Y

F

A

M

I

D

K

R

V

L

L

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D

Q

E

Y

L

L

T

D

D

E

G

G

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D

A

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I

T

I

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S

T

T

G

G

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x
V

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|
G

S
x
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A
x
H

C

Q

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x
M

C

W

S

A

F

A

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A

F

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Y

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I

Y

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F

L

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x
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C

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x
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|
M

G

G

Y

F

G

G

L

L

P

P

A

A

L

I

G

G

A

A

A

S

V

V

A

A

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N

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P

G

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K

A

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I

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I

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L

I

D

D

G
|
G

D
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D

G
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S
|
S

I

F

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I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

N

E

K

N

L

L

N

P

L

V

K

K

I

V

F

L

W

L

L

L

N

N

N
|
N

D

Q

G
x
H

Y
x
L

H
x
G

S
x
M

I
x
V

R

M

Q

Q

T
x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

T

Q

S

E

G

D

I

E

S

I

F

F

P

P

S

N

A

M

D

L

K

L

I

F

A

A

R

A

A

A

Y

C

G

G

F

I

E

P

F

A

I

A

K

R

I

V

D

T

A

K

V

K

E

A

D

D

M

L

D

R

E

E

K

A

I

I

K

Q

K

T

V

M

L

L

L

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S

T

D

P

T

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S

P

I

Y

V

L

C

L

E

D

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V

I

I

active site: Y33 (≠ V24), G35 (= G26), G36 (= G27), A37 (≠ G28), S38 (≠ A29), E59 (= E50), T82 (= T73), F121 (vs. gap), Q122 (= Q121), E123 (= E122), K171 (≠ L170), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (= D456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K134), R161 (= R160), Y191 (≠ D190), R194 (≠ Q193), D291 (≠ I292), R292 (= R293), D312 (= D314), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R160), G222 (= G221), G224 (= G223), T246 (≠ A247), L247 (≠ W248), M248 (≠ N249), L264 (≠ P265), G265 (= G266), M266 (≠ T267), H267 (≠ I268), G286 (= G287), V287 (≠ C288), R288 (= R289), D290 (≠ N291), R292 (= R293), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ L333), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (= D456), N480 (= N483)
binding thiamine diphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ S405), H403 (≠ A406), G426 (≠ A429), M428 (= M431), G452 (= G455), G454 (= G457), S455 (= S458), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ H487), M485 (≠ S488), V486 (≠ I489)

Sites not aligning to the query:

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 13:555/582 of 1yi1A

query
sites
1yi1A

K

K

V

G

S

A

D

D

Y

I

I

L

A

V

S

E

F

A

M

L

V

E

E

R

N

Q

G

G

I

V

T

E

D

T

L

V

F

F

T

A

V
x
Y

T

P

G
|
G

G
x
A

A
x
S

M

M

H

E

L

I

N

H

D

Q

S

A

L

L

G

T

N

R

H

S

P

S

E

S

L

I

V

R

S

N

T

V

Y

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

A

G

Y

Y

A

A

R

R

L

S

T

S

G

G

R

K

I

P

A

G

L

I

V

C

C

I

V

A

T
|
T

S

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

M

A

G

D

G

A

W

L

V

L

D

D

S

S

I

V

P

P

M

L

F

V

V

A

I

I

S

T

G

G

Q

Q

V

-

K

-

F

-

S

-

T

-

I

-

A

-

S

-

T

-

N

-

V

V

P

P

L

R

R

R

Q

M

L

I

G

G

D

T

-

D

-

A

-
x
F

Q
|
Q

E
|
E

F

T

N

P

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I

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E

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V

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K

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M

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I

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A

A

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F

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A

A

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R
|
R

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P

G

G

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P

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L

L

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I

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x
K

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A

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Y

A

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G

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x
G

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|
G

I

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G

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x
M

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x
L

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|
G

T
x
M

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x
H

G

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T

T

R

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G

Y

G

A

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N

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Y

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A

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D

D

L

L

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A

L

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G

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R

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F

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x
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I
x
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|
R

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x
V

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N

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W

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A

F

F

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I
|
I

D

D

A

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A

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I

K

G

K

K

P

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|
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V

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F

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W

W

C

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R

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E

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-

V

-

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-

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Q

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A

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-

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-

P

-

H

S

H

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Y

F

A

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I

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T

D

D

E

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D

A

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I

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S

T

T

G

G

N
x
V

G

G

S

Q

A

H

C

Q

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x
M

C

W

S

A

F

A

Q

Q

A

F

M

Y

V

N

I

Y

K

K

N

P

Q

R

R

Q

L

W

F

L

T

S

N

S

S
x
G

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|
G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

L

I

G

G

A

A

A

S

V

V

A

A

L

N

G

P

G

D

K

A

R

I

V

V

I

V

C

D

L

I

D

D

G

G

D
|
D

G

G

S

S

I

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

N

E

K

N

L

L

N

P

L

V

K

K

I

V

F

L

W

L

L

L

N

N

N
|
N

D

Q

G
x
H

Y
x
L

H

G

S
x
M

I
x
V

R

M

Q

Q

T
x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

T

Q

S

E

G

D

I

E

S

I

F

F

P

P

S

N

A

M

D

L

K

L

I

F

A

A

R

A

A

A

Y

C

G

G

F

I

E

P

F

A

I

A

K

R

I

V

D

T

A

K

V

K

E

A

D

D

M

L

D

R

E

E

K

A

I

I

K

Q

K

T

V

M

L

L

L

D

S

T

D

P

T

G

S

P

I

Y

V

L

C

L

E

D

V

V

I

I

active site: Y33 (≠ V24), G35 (= G26), G36 (= G27), A37 (≠ G28), S38 (≠ A29), E59 (= E50), T82 (= T73), F121 (vs. gap), Q122 (= Q121), E123 (= E122), K171 (≠ L170), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (= D456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (≠ I292), R292 (= R293), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R160), G223 (≠ S222), G224 (= G223), T246 (≠ A247), L247 (≠ W248), M248 (≠ N249), M263 (≠ R264), L264 (≠ P265), G265 (= G266), M266 (≠ T267), H267 (≠ I268), G286 (= G287), V287 (≠ C288), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ L333), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (= D456), N480 (= N483), H482 (≠ G485)

Sites not aligning to the query:

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 13:555/582 of 1yi0A

query
sites
1yi0A

K

K

V

G

S

A

D

D

Y

I

I

L

A

V

S

E

F

A

M

L

V

E

E

R

N

Q

G

G

I

V

T

E

D

T

L

V

F

F

T

A

V
x
Y

T

P

G
|
G

G
x
A

A
x
S

M

M

H

E

L

I

N

H

D

Q

S

A

L

L

G

T

N

R

H

S

P

S

E

S

L

I

V

R

S

N

T

V

Y

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

A

G

Y

Y

A

A

R

R

L

S

T

S

G

G

R

K

I

P

A

G

L

I

V

C

C

I

V

A

T
|
T

S

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

M

A

G

D

G

A

W

L

V

L

D

D

S

S

I

V

P

P

M

L

F

V

V

A

I

I

S

T

G

G

Q

Q

V

-

K

-

F

-

S

-

T

-

I

-

A

-

S

-

T

-

N

-

V

V

P

P

L

R

R

R

Q

M

L

I

G

G

D

T

-

D

-

A

-
x
F

Q
|
Q

E
|
E

F

T

N

P

I

I

V

V

D

E

S

V

V

T

R

R

C

S

M

I

T

T

K

K

Y

H

A

N

V

Y

M

L

L

V

T

M

E

D

P

V

E

E

S

D

I

I

A

P

Y

R

H

I

L

I

E

E

R

E

A

A

L

F

F

F

L

L

A

A

G

T

H

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

L

V

D

D

I

V

P

P

L
x
K

N

D

V

I

Q

Q

G

Q

A

Q

L

L

I

A

E

I

T

P

D

N

A

W

L

E

Q

Q

H

A

Y

M

D

R

P

L

S

P

E

G

D

Y

A

M

V

S

Q

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V

M

V

P

A

K

P

P

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E

N

D

D

S

V

H

Y

L

S

E

Q

Q

L

I

I

V

E

R

K

L

I

I

C

S

N

E

A

S

R

K

K

K

P

P

V

V

I

L

L

Y

A

V

G
|
G

S
x
G

G
|
G

I

C

R

L

L

-

S

-

G

N

S

S

H

S

D

D

V

E

F

L

I

G

Q

R

L

F

L

V

E

E

K

L

L

T

N

G

I

I

P

P

V

V

V

A

T

S

A
x
T

W
x
L

N
x
M

A

G

H

L

D

G

N

S

L

Y

W

P

D

C

D

D

H

D

P

E

L

L

Y

S

C

L

G

H

R

M

P
x
L

G
|
G

T
x
M

I
x
H

G

G

T

T

R

V

G

Y

G

A

N

N

F

Y

V

A

V

V

Q

E

N

H

C

S

D

D

L

L

S

A

L

F

G
|
G

C
x
V

R
|
R

M

F

N
x
D

I
x
D

R
|
R

Q

-

I
x
V

S

T

Y

G

N

K

W

L

E

E

N

A

F

F

A

A

K

S

D

R

A

A

Y

K

L

I

V

V

A

H

V

I

D
|
D

I
|
I

D

D

A

S

G

A

E

E

L

I

K

G

K

K

P

-

T

N

L

K

T

T

V

P

D

H

M

V

P

S

I

V

H

C

A
x
G

D
|
D

L
x
V

G

K

D

L

F

A

L

L

P

Q

G

G

L

M

L

N

G

K

A

V

M

L

Q

E

A

N

D

R

H

A

K

E

L

E

G

L

N

K

I

L

E

D

W

F

L

G

E

V

W

W

C

R

R

N

K

E

I

L

N

N

-

V

-

Q

-

K

Q

Q

K

K

Y

F

P

P

A

L

V

-

Q

-

P

-

H

S

H

F

Y

K

T

T

P

F

H

G

S

E

P

A

V

I

N

P

P

P

Y

Q

V

Y

F

A

M

I

D

K

R

V

L

L

S

D

Q

E

Y

L

L

T

D

D

E

G

G

K

D

A

V

I

T

I

V

S

T

T

G

G

N
x
V

G

G

S

Q

A

H

C

Q

V
x
M

C

W

S

A

F

A

Q

Q

A

F

M

Y

V

N

I

Y

K

K

N

P

Q

R

R

Q

L

W

F

L

T

S

N

S

S
x
G

G
|
G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

L

I

G

G

A

A

A

S

V

V

A

A

L

N

G

P

G

D

K

A

R

I

V

V

I

V

C

D

L

I

D

D

G

G

D
|
D

G

G

S

S

I

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

N

E

K

N

L

L

N

P

L

V

K

K

I

V

F

L

W

L

L

L

N

N

N
|
N

D

Q

G
x
H

Y
x
L

H

G

S
x
M

I
x
V

R

M

Q

Q

T
x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

T

Q

S

E

G

D

I

E

S

I

F

F

P

P

S

N

A

M

D

L

K

L

I

F

A

A

R

A

A

A

Y

C

G

G

F

I

E

P

F

A

I

A

K

R

I

V

D

T

A

K

V

K

E

A

D

D

M

L

D

R

E

E

K

A

I

I

K

Q

K

T

V

M

L

L

L

D

S

T

D

P

T

G

S

P

I

Y

V

L

C

L

E

D

V

V

I

I

active site: Y33 (≠ V24), G35 (= G26), G36 (= G27), A37 (≠ G28), S38 (≠ A29), E59 (= E50), T82 (= T73), F121 (vs. gap), Q122 (= Q121), E123 (= E122), K171 (≠ L170), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (= D456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ I292), R292 (= R293), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R160), G222 (= G221), G223 (≠ S222), G224 (= G223), T246 (≠ A247), L247 (≠ W248), M248 (≠ N249), L264 (≠ P265), G265 (= G266), M266 (≠ T267), H267 (≠ I268), G286 (= G287), V287 (≠ C288), R288 (= R289), D290 (≠ N291), R292 (= R293), V293 (≠ I295), D310 (= D312), I311 (= I313), G328 (≠ A331), D329 (= D332), V330 (≠ L333), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (= D456), N480 (= N483), H482 (≠ G485)

Sites not aligning to the query:

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 13:555/582 of 1yhzA

query
sites
1yhzA

K

K

V

G

S

A

D

D

Y

I

I

L

A

V

S

E

F

A

M

L

V

E

E

R

N

Q

G

G

I

V

T

E

D

T

L

V

F

F

T

A

V
x
Y

T

P

G
|
G

G
x
A

A
x
S

M

M

H

E

L

I

N

H

D

Q

S

A

L

L

G

T

N

R

H

S

P

S

E

S

L

I

V

R

S

N

T

V

Y

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

A

G

Y

Y

A

A

R

R

L

S

T

S

G

G

R

K

I

P

A

G

L

I

V

C

C

I

V

A

T
|
T

S

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

M

A

G

D

G

A

W

L

V

L

D

D

S

S

I

V

P

P

M

L

F

V

V

A

I

I

S

T

G

G

Q

Q

V

-

K

-

F

-

S

-

T

-

I

-

A

-

S

-

T

-

N

-

V

V

P

P

L

R

R

R

Q

M

L

I

G

G

D

T

-

D

-

A

-
x
F

Q
|
Q

E
|
E

F

T

N

P

I

I

V

V

D

E

S

V

V

T

R

R

C

S

M

I

T

T

K

K

Y

H

A

N

V

Y

M

L

L

V

T

M

E

D

P

V

E

E

S

D

I

I

A

P

Y

R

H

I

L

I

E

E

R

E

A

A

L

F

F

F

L

L

A

A

G

T

H

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

L

V

D

D

I

V

P

P

L
x
K

N

D

V

I

Q

Q

G

Q

A

Q

L

L

I

A

E

I

T

P

D

N

A

W

L

E

Q

Q

H

A

Y

M

D

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P

L

S

P

E

G

D

Y

A

M

V

S

Q

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V

M

V

P

A

K

P

P

S

E

N

D

D

S

V

H

Y

L

S

E

Q

Q

L

I

I

V

E

R

K

L

I

I

C

S

N

E

A

S

R

K

K

K

P

P

V

V

I

L

L

Y

A

V

G

G

S
x
G

G
|
G

I

C

R

L

L

-

S

-

G

N

S

S

H

S

D

D

V

E

F

L

I

G

Q

R

L

F

L

V

E

E

K

L

L

T

N

G

I

I

P

P

V

V

V

A

T

S

A
x
T

W
x
L

N
x
M

A

G

H

L

D

G

N

S

L

Y

W

P

D

C

D

D

H

D

P

E

L

L

Y

S

C

L

G

H

R

M

P
x
L

G

G

T
x
M

I
x
H

G

G

T

T

R

V

G

Y

G

A

N

N

F

Y

V

A

V

V

Q

E

N

H

C

S

D

D

L

L

S

A

L

F

G
|
G

C
x
V

R
|
R

M

F

N
x
D

I
x
D

R
|
R

Q

-

I
x
V

S

T

Y

G

N

K

W

L

E

E

N

A

F

F

A

A

K

S

D

R

A

A

Y

K

L

I

V

V

A

H

V

I

D
|
D

I
|
I

D

D

A

S

G

A

E

E

L

I

K

G

K

K

P

-

T

N

L

K

T

T

V

P

D

H

M

V

P

S

I

V

H

C

A

G

D
|
D

L
x
V

G

K

D

L

F

A

L

L

P

Q

G

G

L

M

L

N

G

K

A

V

M

L

Q

E

A

N

D

R

H

A

K

E

L

E

G

L

N

K

I

L

E

D

W

F

L

G

E

V

W

W

C

R

R

N

K

E

I

L

N

N

-

V

-

Q

-

K

Q

Q

K

K

Y

F

P

P

A

L

V

-

Q

-

P

-

H

S

H

F

Y

K

T

T

P

F

H

G

S

E

P

A

V

I

N

P

P

P

Y

Q

V

Y

F

A

M

I

D

K

R

V

L

L

S

D

Q

E

Y

L

L

T

D

D

E

G

G

K

D

A

V

I

T

I

V

S

T

T

G

G

N
x
V

G

G

S

Q

A

H

C
x
Q

V
x
M

C

W

S

A

F

A

Q

Q

A

F

M

Y

V

N

I

Y

K

K

N

P

Q

R

R

Q

L

W

F

L

T

S

N

S

S
x
G

G
|
G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

L

I

G

G

A

A

A

S

V

V

A

A

L

N

G

P

G

D

K

A

R

I

V

V

I

V

C

D

L

I

D

D

G

G

D
|
D

G

G

S

S

I

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

N

E

K

N

L

L

N

P

L

V

K

K

I

V

F

L

W

L

L

L

N

N

N
|
N

D

Q

G
x
H

Y
x
L

H

G

S
x
M

I
x
V

R

M

Q

Q

T
x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

T

Q

S

E

G

D

I

E

S

I

F

F

P

P

S

N

A

M

D

L

K

L

I

F

A

A

R

A

A

A

Y

C

G

G

F

I

E

P

F

A

I

A

K

R

I

V

D

T

A

K

V

K

E

A

D

D

M

L

D

R

E

E

K

A

I

I

K

Q

K

T

V

M

L

L

L

D

S

T

D

P

T

G

S

P

I

Y

V

L

C

L

E

D

V

V

I

I

active site: Y33 (≠ V24), G35 (= G26), G36 (= G27), A37 (≠ G28), S38 (≠ A29), E59 (= E50), T82 (= T73), F121 (vs. gap), Q122 (= Q121), E123 (= E122), K171 (≠ L170), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (= D456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (≠ I292), R292 (= R293), M485 (≠ S488), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R160), G223 (≠ S222), G224 (= G223), T246 (≠ A247), L247 (≠ W248), M248 (≠ N249), L264 (≠ P265), M266 (≠ T267), H267 (≠ I268), G286 (= G287), V287 (≠ C288), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ L333), Q404 (≠ C407), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (= D456), N480 (= N483), H482 (≠ G485)

Sites not aligning to the query:

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 13:555/582 of 1yhyA

query
sites
1yhyA

K

K

V

G

S

A

D

D

Y

I

I

L

A

V

S

E

F

A

M

L

V

E

E

R

N

Q

G

G

I

V

T

E

D

T

L

V

F

F

T

A

V
x
Y

T

P

G
|
G

G
x
A

A
x
S

M

M

H

E

L

I

N

H

D

Q

S

A

L

L

G

T

N

R

H

S

P

S

E

S

L

I

V

R

S

N

T

V

Y

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

A

G

Y

Y

A

A

R

R

L

S

T

S

G

G

R

K

I

P

A

G

L

I

V

C

C

I

V

A

T
|
T

S

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

M

A

G

D

G

A

W

L

V

L

D

D

S

S

I

V

P

P

M

L

F

V

V

A

I

I

S

T

G

G

Q

Q

V

-

K

-

F

-

S

-

T

-

I

-

A

-

S

-

T

-

N

-

V

V

P

P

L

R

R

R

Q

M

L

I

G

G

D

T

-

D

-

A

-
x
F

Q
|
Q

E
|
E

F

T

N

P

I

I

V

V

D

E

S

V

V

T

R

R

C

S

M

I

T

T

K

K

Y

H

A

N

V

Y

M

L

L

V

T

M

E

D

P

V

E

E

S

D

I

I

A

P

Y

R

H

I

L

I

E

E

R

E

A

A

L

F

F

F

L

L

A

A

G

T

H

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

L

V

D

D

I

V

P

P

L
x
K

N

D

V

I

Q

Q

G

Q

A

Q

L

L

I

A

E

I

T

P

D

N

A

W

L

E

Q

Q

H

A

Y

M

D

R

P

L

S

P

E

G

D

Y

A

M

V

S

Q

R

V

M

V

P

A

K

P

P

S

E

N

D

D

S

V

H

Y

L

S

E

Q

Q

L

I

I

V

E

R

K

L

I

I

C

S

N

E

A

S

R

K

K

K

P

P

V

V

I

L

L

Y

A

V

G
|
G

S
x
G

G
|
G

I

C

R

L

L

-

S

-

G

N

S

S

H

S

D

D

V

E

F

L

I

G

Q

R

L

F

L

V

E

E

K

L

L

T

N

G

I

I

P

P

V

V

V

A

T

S

A
x
T

W
x
L

N
x
M

A

G

H

L

D

G

N

S

L

Y

W

P

D

C

D

D

H

D

P

E

L

L

Y

S

C

L

G

H

R

M

P
x
L

G
|
G

T
x
M

I
x
H

G

G

T

T

R

V

G

Y

G

A

N

N

F

Y

V

A

V

V

Q

E

N

H

C

S

D

D

L

L

S

A

L

F

G
|
G

C
x
V

R
|
R

M

F

N
x
D

I
x
D

R
|
R

Q

-

I
x
V

S

T

Y

G

N

K

W

L

E

E

N

A

F

F

A

A

K

S

D

R

A

A

Y

K

L

I

V

V

A

H

V

I

D
|
D

I
|
I

D

D

A

S

G

A

E

E

L

I

K

G

K

K

P

-

T

N

L

K

T

T

V

P

D

H

M

V

P

S

I

V

H

C

A

G

D
|
D

L
x
V

G

K

D

L

F

A

L

L

P

Q

G

G

L

M

L

N

G

K

A

V

M

L

Q

E

A

N

D

R

H

A

K

E

L

E

G

L

N

K

I

L

E

D

W

F

L

G

E

V

W

W

C

R

R

N

K

E

I

L

N

N

-

V

-

Q

-

K

Q

Q

K

K

Y

F

P

P

A

L

V

-

Q

-

P

-

H

S

H

F

Y

K

T

T

P

F

H

G

S

E

P

A

V

I

N

P

P

P

Y

Q

V

Y

F

A

M

I

D

K

R

V

L

L

S

D

Q

E

Y

L

L

T

D

D

E

G

G

K

D

A

V

I

T

I

V

S

T

T

G

G

N
x
V

G

G

S

Q

A

H

C
x
Q

V
x
M

C

W

S

A

F

A

Q

Q

A

F

M

Y

V

N

I

Y

K

K

N

P

Q

R

R

Q

L

W

F

L

T

S

N

S

S
x
G

G
|
G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

L

I

G

G

A

A

A

S

V

V

A

A

L

N

G

P

G

D

K

A

R

I

V

V

I

V

C

D

L

I

D

D

G

G

D
|
D

G

G

S

S

I

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

N

E

K

N

L

L

N

P

L

V

K

K

I

V

F

L

W

L

L

L

N

N

N
|
N

D

Q

G
x
H

Y
x
L

H

G

S
x
M

I
x
V

R

M

Q

Q

T
x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

T

Q

S

E

G

D

I

E

S

I

F

F

P

P

S

N

A

M

D

L

K

L

I

F

A

A

R

A

A

A

Y

C

G

G

F

I

E

P

F

A

I

A

K

R

I

V

D

T

A

K

V

K

E

A

D

D

M

L

D

R

E

E

K

A

I

I

K

Q

K

T

V

M

L

L

L

D

S

T

D

P

T

G

S

P

I

Y

V

L

C

L

E

D

V

V

I

I

active site: Y33 (≠ V24), G35 (= G26), G36 (= G27), A37 (≠ G28), S38 (≠ A29), E59 (= E50), T82 (= T73), F121 (vs. gap), Q122 (= Q121), E123 (= E122), K171 (≠ L170), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (= D456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ I292), R292 (= R293), V486 (≠ I489), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R160), G222 (= G221), G223 (≠ S222), G224 (= G223), T246 (≠ A247), L247 (≠ W248), M248 (≠ N249), L264 (≠ P265), G265 (= G266), M266 (≠ T267), H267 (≠ I268), G286 (= G287), V287 (≠ C288), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ L333), Q404 (≠ C407), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (= D456), N480 (= N483), H482 (≠ G485)

Sites not aligning to the query:

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 13:555/582 of 1ybhA

query
sites
1ybhA

K

K

V

G

S

A

D

D

Y

I

I

L

A

V

S

E

F

A

M

L

V

E

E

R

N

Q

G

G

I

V

T

E

D

T

L

V

F

F

T

A

V
x
Y

T

P

G
|
G

G
x
A

A
x
S

M

M

H

E

L

I

N

H

D

Q

S

A

L

L

G

T

N

R

H

S

P

S

E

S

L

I

V

R

S

N

T

V

Y

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

A

G

Y

Y

A

A

R

R

L

S

T

S

G

G

R

K

I

P

A

G

L

I

V

C

C

I

V

A

T
|
T

S

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

M

A

G

D

G

A

W

L

V

L

D

D

S

S

I

V

P

P

M

L

F

V

V

A

I

I

S

T

G

G

Q

Q

V

-

K

-

F

-

S

-

T

-

I

-

A

-

S

-

T

-

N

-

V

V

P

P

L

R

R

R

Q

M

L

I

G

G

D

T

-

D

-

A

-
x
F

Q
|
Q

E
|
E

F

T

N

P

I

I

V

V

D

E

S

V

V

T

R

R

C

S

M

I

T

T

K

K

Y

H

A

N

V

Y

M

L

L

V

T

M

E

D

P

V

E

E

S

D

I

I

A

P

Y

R

H

I

L

I

E

E

R

E

A

A

L

F

F

F

L

L

A

A

G

T

H

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

L

V

D

D

I

V

P

P

L
x
K

N

D

V

I

Q

Q

G

Q

A

Q

L

L

I

A

E

I

T

P

D

N

A

W

L

E

Q

Q

H

A

Y

M

D

R

P

L

S

P

E

G

D

Y

A

M

V

S

Q

R

V

M

V

P

A

K

P

P

S

E

N

D

D

S

V

H

Y

L

S

E

Q

Q

L

I

I

V

E

R

K

L

I

I

C

S

N

E

A

S

R

K

K

K

P

P

V

V

I

L

L

Y

A

V

G

G

S
x
G

G
|
G

I

C

R

L

L

-

S

-

G

N

S

S

H

S

D

D

V

E

F

L

I

G

Q

R

L

F

L

V

E

E

K

L

L

T

N

G

I

I

P

P

V

V

V

A

T

S

A
x
T

W
x
L

N
x
M

A

G

H

L

D

G

N

S

L

Y

W

P

D

C

D

D

H

D

P

E

L

L

Y

S

C

L

G

H

R

M

P
x
L

G

G

T
x
M

I
x
H

G

G

T

T

R

V

G

Y

G

A

N

N

F

Y

V

A

V

V

Q

E

N

H

C

S

D

D

L

L

S

A

L

F

G
|
G

C
x
V

R
|
R

M

F

N
x
D

I
x
D

R
|
R

Q

-

I
x
V

S

T

Y

G

N

K

W

L

E

E

N

A

F

F

A

A

K

S

D

R

A

A

Y

K

L

I

V

V

A

H

V

I

D
|
D

I
|
I

D

D

A

S

G

A

E

E

L

I

K

G

K

K

P

-

T

N

L

K

T

T

V

P

D

H

M

V

P

S

I

V

H

C

A

G

D
|
D

L
x
V

G

K

D

L

F

A

L

L

P

Q

G

G

L

M

L

N

G

K

A

V

M

L

Q

E

A

N

D

R

H

A

K

E

L

E

G

L

N

K

I

L

E

D

W

F

L

G

E

V

W

W

C

R

R

N

K

E

I

L

N

N

-

V

-

Q

-

K

Q

Q

K

K

Y

F

P

P

A

L

V

-

Q

-

P

-

H

S

H

F

Y

K

T

T

P

F

H

G

S

E

P

A

V

I

N

P

P

P

Y

Q

V

Y

F

A

M

I

D

K

R

V

L

L

S

D

Q

E

Y

L

L

T

D

D

E

G

G

K

D

A

V

I

T

I

V

S

T

T

G

G

N
x
V

G

G

S

Q

A

H

C
x
Q

V
x
M

C

W

S

A

F

A

Q

Q

A

F

M

Y

V

N

I

Y

K

K

N

P

Q

R

R

Q

L

W

F

L

T

S

N

S

S
x
G

G
|
G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

L

I

G

G

A

A

A

S

V

V

A

A

L

N

G

P

G

D

K

A

R

I

V

V

I

V

C

D

L

I

D

D

G

G

D
|
D

G

G

S

S

I

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

N

E

K

N

L

L

N

P

L

V

K

K

I

V

F

L

W

L

L

L

N

N

N
|
N

D

Q

G
x
H

Y
x
L

H

G

S
x
M

I
x
V

R

M

Q

Q

T
x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

T

Q

S

E

G

D

I

E

S

I

F

F

P

P

S

N

A

M

D

L

K

L

I

F

A

A

R

A

A

A

Y

C

G

G

F

I

E

P

F

A

I

A

K

R

I

V

D

T

A

K

V

K

E

A

D

D

M

L

D

R

E

E

K

A

I

I

K

Q

K

T

V

M

L

L

L

D

S

T

D

P

T

G

S

P

I

Y

V

L

C

L

E

D

V

V

I

I

active site: Y33 (≠ V24), G35 (= G26), G36 (= G27), A37 (≠ G28), S38 (≠ A29), E59 (= E50), T82 (= T73), F121 (vs. gap), Q122 (= Q121), E123 (= E122), K171 (≠ L170), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (= D456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (≠ T267), D291 (≠ I292), R292 (= R293), M485 (≠ S488), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R160), G223 (≠ S222), G224 (= G223), T246 (≠ A247), L247 (≠ W248), M248 (≠ N249), L264 (≠ P265), M266 (≠ T267), H267 (≠ I268), G286 (= G287), V287 (≠ C288), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ L333), Q404 (≠ C407), M405 (≠ V408), G423 (≠ S426), G424 (= G427)
binding magnesium ion: D453 (= D456), N480 (= N483), H482 (≠ G485)

Sites not aligning to the query:

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 13:555/583 of 5k3sA

query
sites
5k3sA

K

K

V

G

S

A

D

D

Y

I

I

L

A

V

S

E

F

A

M

L

V

E

E

R

N

Q

G

G

I

V

T

E

D

T

L

V

F

F

T

A

V
x
Y

T

P

G
|
G

G
x
A

A
x
S

M

M

H

E

L

I

N

H

D

Q

S

A

L

L

G

T

N

R

H

S

P

S

E

S

L

I

V

R

S

N

T

V

Y

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

A

G

Y

Y

A

A

R

R

L

S

T

S

G

G

R

K

I

P

A

G

L

I

V

C

C

I

V

A

T
|
T

S

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

M

A

G

D

G

A

W

L

V

L

D

D

S

S

I

V

P

P

M

L

F

V

V

A

I

I

S

T

G

G

Q

Q

V

-

K

-

F

-

S

-

T

-

I

-

A

-

S

-

T

-

N

-

V

V

P

P

L

R

R

R

Q

M

L

I

G

G

D

T

-

D

-

A

-
x
F

Q
|
Q

E
|
E

F

T

N

P

I

I

V

V

D

E

S

V

V

T

R

R

C

S

M

I

T

T

K

K

Y

H

A

N

V

Y

M

L

L

V

T

M

E

D

P

V

E

E

S

D

I

I

A

P

Y

R

H

I

L

I

E

E

R

E

A

A

L

F

F

F

L

L

A

A

G

T

H

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

L

V

D

D

I

V

P

P

L
x
K

N

D

V

I

Q

Q

G

Q

A

Q

L

L

I

A

E

I

T

P

D

N

A

W

L

E

Q

Q

H

A

Y

M

D

R

P

L

S

P

E

G

D

Y

A

M

V

S

Q

R

V

M

V

P

A

K

P

P

S

E

N

D

D

S

V

H

Y

L

S

E

Q

Q

L

I

I

V

E

R

K

L

I

I

C

S

N

E

A

S

R

K

K

K

P

P

V

V

I

L

L

Y

A

V

G
|
G

S
x
G

G
|
G

I

C

R

L

L

-

S

-

G

N

S

S

H

S

D

D

V

E

F

L

I

G

Q

R

L

F

L

V

E

E

K

L

L

T

N

G

I

I

P

P

V

V

V

A

T

S

A
x
T

W
x
L

N
x
M

A

G

H

L

D

G

N

S

L

Y

W

P

D

C

D

D

H

D

P

E

L

L

Y

S

C

L

G

H

R

M

P
x
L

G

G

T
x
M

I

H

G

G

T

T

R

V

G

Y

G

A

N

N

F

Y

V

A

V

V

Q

E

N

H

C

S

D

D

L

L

S

A

L

F

G
|
G

C

V

R
|
R

M

F

N
x
D

I

D

R
|
R

Q

-

I
x
V

S

T

Y

G

N

K

W

L

E

E

N

A

F

F

A

A

K

S

D

R

A

A

Y

K

L

I

V

V

A

H

V

I

D
|
D

I
|
I

D

D

A

S

G

A

E

E

L

I

K

G

K

K

P

-

T

N

L

K

T

T

V

P

D

H

M

V

P

S

I

V

H

C

A

G

D
|
D

L
x
V

G

K

D

L

F

A

L

L

P

Q

G

G

L

M

L

N

G

K

A

V

M

L

Q

E

A

N

D

R

H

A

K

E

L

E

G

L

N

K

I

L

E

D

W

F

L

G

E

V

W

W

C

R

R

N

K

E

I

L

N

N

-

V

-

Q

-

K

Q

Q

K

K

Y

F

P

P

A

L

V

-

Q

-

P

-

H

S

H

F

Y

K

T

T

P

F

H

G

S

E

P

A

V

I

N

P

P

P

Y

Q

V

Y

F

A

M

I

D

K

R

V

L

L

S

D

Q

E

Y

L

L

T

D

D

E

G

G

K

D

A

V

I

T

I

V

S

T

T

G

G

N
x
V

G
|
G

S
x
Q

A
x
H

C

Q

V
x
M

C

W

S

A

F

A

Q

Q

A

F

M

Y

V

N

I

Y

K

K

N

P

Q

R

R

Q

L

W

F

L

T

S

N

S

S
x
G

G

G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

L

I

G

G

A

A

A

S

V

V

A

A

L

N

G

P

G

D

K

A

R

I

V

V

I

V

C

D

L

I

D

D

G

G

D
|
D

G
|
G

S
|
S

I

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

N

E

K

N

L

L

N

P

L

V

K

K

I

V

F

L

W

L

L

L

N

N

N
|
N

D

Q

G
x
H

Y
x
L

H
x
G

S
x
M

I
x
V

R

M

Q

Q

T
x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

T

Q

S

E

G

D

I

E

S

I

F

F

P

P

S

N

A

M

D

L

K

L

I

F

A

A

R

A

A

A

Y

C

G

G

F

I

E

P

F

A

I

A

K

R

I

V

D

T

A

K

V

K

E

A

D

D

M

L

D

R

E

E

K

A

I

I

K

Q

K

T

V

M

L

L

L

D

S

T

D

P

T

G

S

P

I

Y

V

L

C

L

E

D

V

V

I

I

active site: Y33 (≠ V24), G35 (= G26), G36 (= G27), A37 (≠ G28), S38 (≠ A29), E59 (= E50), T82 (= T73), F121 (vs. gap), Q122 (= Q121), E123 (= E122), K171 (≠ L170), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (= D456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R293), M485 (≠ S488), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R160), G222 (= G221), G223 (≠ S222), G224 (= G223), T246 (≠ A247), L247 (≠ W248), M248 (≠ N249), L264 (≠ P265), M266 (≠ T267), G286 (= G287), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ L333), M405 (≠ V408), G423 (≠ S426)
binding magnesium ion: D453 (= D456), N480 (= N483), H482 (≠ G485)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ S405), H403 (≠ A406), G426 (≠ A429), M428 (= M431), D453 (= D456), G454 (= G457), S455 (= S458), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ H487), M485 (≠ S488), V486 (≠ I489)

Sites not aligning to the query:

active site: 558
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: 569

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
29% identity, 92% coverage: 4:554/598 of query aligns to 13:555/585 of 5k2oA

query
sites
5k2oA

K

K

V

G

S

A

D

D

Y

I

I

L

A

V

S

E

F

A

M

L

V

E

E

R

N

Q

G

G

I

V

T

E

D

T

L

V

F

F

T

A

V
x
Y

T

P

G
|
G

G
x
A

A
x
S

M

M

H

E

L

I

N

H

D

Q

S

A

L

L

G

T

N

R

H

S

P

S

E

S

L

I

V

R

S

N

T

V

Y

L

N

P

H

R

H

H

E
|
E

Q

Q

A

G

C

G

A

V

I

F

A

A

E

E

A

G

Y

Y

A

A

R

R

L

S

T

S

G

G

R

K

I

P

A

G

L

I

V

C

C

I

V

A

T
|
T

S

S

G

G

P

P

G

G

G

A

T

T

N

N

A

L

I

V

T

S

G

G

V

L

M

A

G

D

G

A

W

L

V

L

D

D

S

S

I

V

P

P

M

L

F

V

V

A

I

I

S

T

G

G

Q

Q

V

-

K

-

F

-

S

-

T

-

I

-

A

-

S

-

T

-

N

-

V

V

P

P

L

R

R

R

Q

M

L

I

G

G

D

T

-

D

-

A

-
x
F

Q
|
Q

E
|
E

F

T

N

P

I

I

V

V

D

E

S

V

V

T

R

R

C

S

M

I

T

T

K

K

Y

H

A

N

V

Y

M

L

L

V

T

M

E

D

P

V

E

E

S

D

I

I

A

P

Y

R

H

I

L

I

E

E

R

E

A

A

L

F

F

F

L

L

A

A

G

T

H

S

G

G

R
|
R

P

P

G

G

P

P

V

V

W

L

L

V

D

D

I

V

P

P

L
x
K

N

D

V

I

Q

Q

G

Q

A

Q

L

L

I

A

E

I

T

P

D

N

A

W

L

E

Q

Q

H

A

Y

M

D

R

P

L

S

P

E

G

D

Y

A

M

V

S

Q

R

V

M

V

P

A

K

P

P

S

E

N

D

D

S

V

H

Y

L

S

E

Q

Q

L

I

I

V

E

R

K

L

I

I

C

S

N

E

A

S

R

K

K

K

P

P

V

V

I

L

L

Y

A

V

G
|
G

S
x
G

G
|
G

I

C

R

L

L

-

S

-

G

N

S

S

H

S

D

D

V

E

F

L

I

G

Q

R

L

F

L

V

E

E

K

L

L

T

N

G

I

I

P

P

V

V

V

A

T

S

A
x
T

W
x
L

N
x
M

A

G

H

L

D

G

N

S

L

Y

W

P

D

C

D

D

H

D

P

E

L

L

Y

S

C

L

G

H

R

M

P
x
L

G

G

T
x
M

I

H

G

G

T

T

R

V

G

Y

G

A

N

N

F

Y

V

A

V

V

Q

E

N

H

C

S

D

D

L

L

S

A

L

F

G
|
G

C

V

R
|
R

M

F

N
x
D

I

D

R
|
R

Q

-

I
x
V

S

T

Y

G

N

K

W

L

E

E

N

A

F

F

A

A

K

S

D

R

A

A

Y

K

L

I

V

V

A

H

V

I

D
|
D

I
|
I

D

D

A

S

G

A

E

E

L

I

K

G

K

K

P

-

T

N

L

K

T

T

V

P

D

H

M

V

P

S

I

V

H

C

A

G

D
|
D

L
x
V

G

K

D

L

F

A

L

L

P

Q

G

G

L

M

L

N

G

K

A

V

M

L

Q

E

A

N

D

R

H

A

K

E

L

E

G

L

N

K

I

L

E

D

W

F

L

G

E

V

W

W

C

R

R

N

K

E

I

L

N

N

-

V

-

Q

-

K

Q

Q

K

K

Y

F

P

P

A

L

V

-

Q

-

P

-

H

S

H

F

Y

K

T

T

P

F

H

G

S

E

P

A

V

I

N

P

P

P

Y

Q

V

Y

F

A

M

I

D

K

R

V

L

L

S

D

Q

E

Y

L

L

T

D

D

E

G

G

K

D

A

V

I

T

I

V

S

T

T

G

G

N
x
V

G
|
G

S
x
Q

A
x
H

C
x
Q

V
x
M

C

W

S

A

F

A

Q

Q

A

F

M

Y

V

N

I

Y

K

K

N

P

Q

R

R

Q

L

W

F

L

T

S

N

S

S
x
G

G

G

C

L

A
x
G

S

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

L

I

G

G

A

A

A

S

V

V

A

A

L

N

G

P

G

D

K

A

R

I

V

V

I

V

C

D

L

I

D

D

G

G

D
|
D

G
|
G

S
|
S

I

F

Q

I

M

M

N

N

L

V

Q

Q

E

E

L

L

Q

A

T

T

I

I

I

R

H

V

N

E

K

N

L

L

N

P

L

V

K

K

I

V

F

L

W

L

L

L

N

N

N
|
N

D

Q

G
x
H

Y
x
L

H
x
G

S
x
M

I
x
V

R

M

Q

Q

T
x
W

Q

E

T

D

N

R

I

F

F

Y

N

K

A

A

R

N

-

R

-

A

-

H

-

T

F

F

C

L

G

G

V

D

N

P

E

A

T

Q

S

E

G

D

I

E

S

I

F

F

P

P

S

N

A

M

D

L

K

L

I

F

A

A

R

A

A

A

Y

C

G

G

F

I

E

P

F

A

I

A

K

R

I

V

D

T

A

K

V

K

E

A

D

D

M

L

D

R

E

E

K

A

I

I

K

Q

K

T

V

M

L

L

L

D

S

T

D

P

T

G

S

P

I

Y

V

L

C

L

E

D

V

V

I

I

active site: Y33 (≠ V24), G35 (= G26), G36 (= G27), A37 (≠ G28), S38 (≠ A29), E59 (= E50), T82 (= T73), F121 (vs. gap), Q122 (= Q121), E123 (= E122), K171 (≠ L170), M266 (≠ T267), V293 (≠ I295), V400 (≠ N403), G426 (≠ A429), M428 (= M431), D453 (= D456), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), M485 (≠ S488), V486 (≠ I489), W489 (≠ T492)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (≠ T267), R292 (= R293), W489 (≠ T492)
binding flavin-adenine dinucleotide: R161 (= R160), G222 (= G221), G223 (≠ S222), G224 (= G223), T246 (≠ A247), L247 (≠ W248), M248 (≠ N249), L264 (≠ P265), G286 (= G287), R288 (= R289), D290 (≠ N291), V293 (≠ I295), D310 (= D312), I311 (= I313), D329 (= D332), V330 (≠ L333), Q404 (≠ C407), M405 (≠ V408), G423 (≠ S426)
binding magnesium ion: D453 (= D456), N480 (= N483), H482 (≠ G485)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ N403), G401 (= G404), Q402 (≠ S405), H403 (≠ A406), M428 (= M431), D453 (= D456), G454 (= G457), S455 (= S458), N480 (= N483), H482 (≠ G485), L483 (≠ Y486), G484 (≠ H487), M485 (≠ S488), V486 (≠ I489)

Sites not aligning to the query:

active site: 558
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: 568

Query Sequence

>WP_012468868.1 NCBI__GCF_000020385.1:WP_012468868.1
MEMKVSDYIASFMVENGITDLFTVTGGGAMHLNDSLGNHPELVSTYNHHEQACAIAAEAY
ARLTGRIALVCVTSGPGGTNAITGVMGGWVDSIPMFVISGQVKFSTTIASTNVPLRQLGD
QEFNIVDSVRCMTKYAVMLTEPESIAYHLERALFLAGHGRPGPVWLDIPLNVQGALIETD
ALQHYDPSEDAVQVVAPPSNDVYSQLIEKICNARKPVILAGSGIRLSGSHDVFIQLLEKL
NIPVVTAWNAHDNLWDDHPLYCGRPGTIGTRGGNFVVQNCDLLLSLGCRMNIRQISYNWE
NFAKDAYLVAVDIDAGELKKPTLTVDMPIHADLGDFLPGLLGAMQADHKLGNIEWLEWCR
KINQKYPAVQPHHYTPHSPVNPYVFMDRLSQYLDEGDVTVTGNGSACVCSFQAMVIKKNQ
RLFTNSGCASMGYGLPAALGAAVALGGKRVICLDGDGSIQMNLQELQTIIHNKLNLKIFW
LNNDGYHSIRQTQTNIFNARFCGVNETSGISFPSADKIARAYGFEFIKIDAVEDMDEKIK
KVLLSDTSIVCEVILNPAQGFEPKLSSRILQDGTMVSPSLEDMFPFLSAEEMKMNAYK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory