SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012469185.1 NCBI__GCF_000020385.1:WP_012469185.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6lpiB Crystal structure of ahas holo-enzyme (see paper)
34% identity, 97% coverage: 4:549/562 of query aligns to 9:531/539 of 6lpiB

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active site: I27 (≠ V22), G29 (= G24), G30 (= G25), S31 (≠ A26), I32 (= I27), E53 (= E47), C76 (≠ T70), F115 (≠ I109), Q116 (= Q110), E117 (= E111), K165 (≠ A164), M256 (≠ F256), A283 (≠ V285), V375 (≠ N393), G401 (≠ A419), M403 (= M421), D428 (= D446), N455 (= N473), A457 (≠ M475), L458 (= L476), L460 (≠ M478), V461 (= V479), Q464 (≠ G482)
binding flavin-adenine dinucleotide: R155 (= R154), G212 (= G210), G213 (≠ W211), G214 (= G212), T236 (≠ S236), L237 (≠ P237), M238 (≠ K238), L254 (= L254), M256 (≠ F256), H257 (≠ A257), G276 (= G278), A277 (≠ T279), R278 (≠ S280), D280 (≠ N282), R282 (≠ F284), A283 (≠ V285), D300 (= D303), I301 (= I304), D319 (= D322), V320 (≠ A323), M380 (≠ A398), G398 (≠ L416)
binding magnesium ion: D428 (= D446), N455 (= N473)
binding thiamine diphosphate: E53 (= E47), C76 (≠ T70), P79 (= P73), G376 (= G394), Q377 (≠ N395), H378 (≠ S396), G401 (≠ A419), M403 (= M421), G427 (= G445), D428 (= D446), G429 (= G447), S430 (= S448), M433 (= M451), N455 (= N473), A457 (≠ M475), L458 (= L476), G459 (= G477), L460 (≠ M478), V461 (= V479)

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
33% identity, 97% coverage: 4:546/562 of query aligns to 97:642/667 of P09342

query
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P09342

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C161 (= C67) modified: Disulfide link with 307
P194 (≠ S100) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ T213) modified: Disulfide link with 161

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
34% identity, 97% coverage: 4:546/562 of query aligns to 94:639/664 of P09114

query
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P09114

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P191 (≠ S100) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (vs. gap) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

6wo1A Hybrid acetohydroxyacid synthase complex structure with cryptococcus neoformans ahas catalytic subunit and saccharomyces cerevisiae ahas regulatory subunit (see paper)
32% identity, 98% coverage: 1:550/562 of query aligns to 9:534/551 of 6wo1A

query
sites
6wo1A

M

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active site: Y30 (≠ V22), G32 (= G24), G33 (= G25), A34 (= A26), I35 (= I27), E56 (= E47), T79 (= T70), F118 (≠ I109), Q119 (= Q110), E120 (= E111), K168 (≠ A164), M255 (≠ F256), V282 (= V285), V398 (vs. gap), G424 (≠ A419), M426 (= M421), D451 (= D446), N478 (= N473)
binding 2-methylpyrimidin-4-amine: G424 (≠ A419), T425 (≠ S420), M426 (= M421)
binding diphosphate: V398 (vs. gap), G399 (= G394), Q400 (≠ N395), H401 (≠ S396), G450 (= G445), D451 (= D446), A452 (≠ G447), S453 (= S448)
binding flavin-adenine dinucleotide: D97 (= D88), R158 (= R154), G208 (= G210), G210 (= G212), S213 (≠ L215), T235 (≠ S236), L236 (≠ P237), Q237 (≠ K238), I253 (≠ L254), G254 (= G255), M255 (≠ F256), G275 (= G278), V276 (≠ T279), R277 (≠ S280), D279 (≠ N282), R281 (≠ F284), V282 (= V285), E308 (≠ D303), I309 (= I304), D327 (= D322), V328 (≠ A323), Q402 (≠ M397), G421 (≠ L416), G422 (= G417)
binding magnesium ion: D451 (= D446), N478 (= N473)

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 14:559/582 of 3ea4A

query
sites
3ea4A

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active site: Y32 (≠ V22), G34 (= G24), G35 (= G25), A36 (= A26), S37 (≠ I27), E58 (= E47), T81 (= T70), F120 (≠ I109), Q121 (= Q110), E122 (= E111), K170 (≠ A164), M265 (≠ F256), V292 (= V285), V399 (≠ N393), G425 (≠ A419), M427 (= M421), D452 (= D446), N479 (= N473), H481 (≠ M475), L482 (= L476), M484 (= M478), V485 (= V479), W488 (vs. gap), H557 (≠ P544)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (≠ E283), R291 (≠ F284), W488 (vs. gap)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R154), G221 (= G210), G222 (= G212), G223 (≠ T213), T245 (≠ S236), L246 (≠ P237), M247 (≠ K238), L263 (= L254), G264 (= G255), M265 (≠ F256), H266 (≠ A257), G285 (= G278), R287 (≠ S280), D289 (≠ N282), R291 (≠ F284), D309 (= D303), I310 (= I304), G327 (= G321), D328 (= D322), V329 (≠ A323), M404 (≠ A398), G422 (≠ L416)
binding magnesium ion: D452 (= D446), N479 (= N473), H481 (≠ M475)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ N393), G400 (= G394), Q401 (≠ N395), H402 (≠ S396), M427 (= M421), G451 (= G445), D452 (= D446), G453 (= G447), S454 (= S448), N479 (= N473), H481 (≠ M475), L482 (= L476), G483 (= G477), M484 (= M478), V485 (= V479)

Sites not aligning to the query:

binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: 567

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 14:559/582 of 3e9yA

query
sites
3e9yA

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-

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active site: Y32 (≠ V22), G34 (= G24), G35 (= G25), A36 (= A26), S37 (≠ I27), E58 (= E47), T81 (= T70), F120 (≠ I109), Q121 (= Q110), E122 (= E111), K170 (≠ A164), M265 (≠ F256), V292 (= V285), V399 (≠ N393), G425 (≠ A419), M427 (= M421), D452 (= D446), N479 (= N473), H481 (≠ M475), L482 (= L476), M484 (= M478), V485 (= V479), W488 (vs. gap), H557 (≠ P544)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (≠ E283), R291 (≠ F284), W488 (vs. gap)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R154), G221 (= G210), G222 (= G212), G223 (≠ T213), T245 (≠ S236), L246 (≠ P237), M247 (≠ K238), L263 (= L254), G285 (= G278), R287 (≠ S280), D289 (≠ N282), R291 (≠ F284), D309 (= D303), I310 (= I304), G327 (= G321), D328 (= D322), V329 (≠ A323), M404 (≠ A398), G422 (≠ L416)
binding magnesium ion: D452 (= D446), N479 (= N473), H481 (≠ M475)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ N393), G400 (= G394), Q401 (≠ N395), H402 (≠ S396), M427 (= M421), G451 (= G445), G453 (= G447), S454 (= S448), N479 (= N473), H481 (≠ M475), L482 (= L476), G483 (= G477), M484 (= M478), V485 (= V479)

Sites not aligning to the query:

binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: 567

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 15:560/582 of 7tzzA

query
sites
7tzzA

A

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S

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binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (≠ F256), R292 (≠ F284), W489 (vs. gap)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ N393), G401 (= G394), Q402 (≠ N395), H403 (≠ S396), G426 (≠ A419), M428 (= M421), G452 (= G445), D453 (= D446), G454 (= G447), S455 (= S448), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G210), G223 (= G212), G224 (≠ T213), T246 (≠ S236), L247 (≠ P237), M248 (≠ K238), M263 (≠ V253), L264 (= L254), M266 (≠ F256), H267 (≠ A257), G286 (= G278), R288 (≠ S280), V293 (= V285), D310 (= D303), I311 (= I304), D329 (= D322), V330 (≠ A323), M405 (≠ A398), G423 (≠ L416)
binding magnesium ion: A37 (= A26), T82 (= T70), S83 (≠ A71), Q122 (= Q110), Y381 (≠ R374), D453 (= D446), M458 (= M451), Q461 (≠ M454), N480 (= N473), H482 (≠ M475), K533 (≠ P518)

Sites not aligning to the query:

binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: 568

6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl (see paper)
31% identity, 98% coverage: 1:548/562 of query aligns to 12:560/597 of 6demA

query
sites
6demA

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