SitesBLAST
Comparing WP_012469185.1 NCBI__GCF_000020385.1:WP_012469185.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6lpiB Crystal structure of ahas holo-enzyme (see paper)
34% identity, 97% coverage: 4:549/562 of query aligns to 9:531/539 of 6lpiB
- active site: I27 (≠ V22), G29 (= G24), G30 (= G25), S31 (≠ A26), I32 (= I27), E53 (= E47), C76 (≠ T70), F115 (≠ I109), Q116 (= Q110), E117 (= E111), K165 (≠ A164), M256 (≠ F256), A283 (≠ V285), V375 (≠ N393), G401 (≠ A419), M403 (= M421), D428 (= D446), N455 (= N473), A457 (≠ M475), L458 (= L476), L460 (≠ M478), V461 (= V479), Q464 (≠ G482)
- binding flavin-adenine dinucleotide: R155 (= R154), G212 (= G210), G213 (≠ W211), G214 (= G212), T236 (≠ S236), L237 (≠ P237), M238 (≠ K238), L254 (= L254), M256 (≠ F256), H257 (≠ A257), G276 (= G278), A277 (≠ T279), R278 (≠ S280), D280 (≠ N282), R282 (≠ F284), A283 (≠ V285), D300 (= D303), I301 (= I304), D319 (= D322), V320 (≠ A323), M380 (≠ A398), G398 (≠ L416)
- binding magnesium ion: D428 (= D446), N455 (= N473)
- binding thiamine diphosphate: E53 (= E47), C76 (≠ T70), P79 (= P73), G376 (= G394), Q377 (≠ N395), H378 (≠ S396), G401 (≠ A419), M403 (= M421), G427 (= G445), D428 (= D446), G429 (= G447), S430 (= S448), M433 (= M451), N455 (= N473), A457 (≠ M475), L458 (= L476), G459 (= G477), L460 (≠ M478), V461 (= V479)
P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
33% identity, 97% coverage: 4:546/562 of query aligns to 97:642/667 of P09342
- C161 (= C67) modified: Disulfide link with 307
- P194 (≠ S100) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
- C307 (≠ T213) modified: Disulfide link with 161
P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
34% identity, 97% coverage: 4:546/562 of query aligns to 94:639/664 of P09114
- P191 (≠ S100) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
- W568 (vs. gap) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.
6wo1A Hybrid acetohydroxyacid synthase complex structure with cryptococcus neoformans ahas catalytic subunit and saccharomyces cerevisiae ahas regulatory subunit (see paper)
32% identity, 98% coverage: 1:550/562 of query aligns to 9:534/551 of 6wo1A
- active site: Y30 (≠ V22), G32 (= G24), G33 (= G25), A34 (= A26), I35 (= I27), E56 (= E47), T79 (= T70), F118 (≠ I109), Q119 (= Q110), E120 (= E111), K168 (≠ A164), M255 (≠ F256), V282 (= V285), V398 (vs. gap), G424 (≠ A419), M426 (= M421), D451 (= D446), N478 (= N473)
- binding 2-methylpyrimidin-4-amine: G424 (≠ A419), T425 (≠ S420), M426 (= M421)
- binding diphosphate: V398 (vs. gap), G399 (= G394), Q400 (≠ N395), H401 (≠ S396), G450 (= G445), D451 (= D446), A452 (≠ G447), S453 (= S448)
- binding flavin-adenine dinucleotide: D97 (= D88), R158 (= R154), G208 (= G210), G210 (= G212), S213 (≠ L215), T235 (≠ S236), L236 (≠ P237), Q237 (≠ K238), I253 (≠ L254), G254 (= G255), M255 (≠ F256), G275 (= G278), V276 (≠ T279), R277 (≠ S280), D279 (≠ N282), R281 (≠ F284), V282 (= V285), E308 (≠ D303), I309 (= I304), D327 (= D322), V328 (≠ A323), Q402 (≠ M397), G421 (≠ L416), G422 (= G417)
- binding magnesium ion: D451 (= D446), N478 (= N473)
3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 14:559/582 of 3ea4A
- active site: Y32 (≠ V22), G34 (= G24), G35 (= G25), A36 (= A26), S37 (≠ I27), E58 (= E47), T81 (= T70), F120 (≠ I109), Q121 (= Q110), E122 (= E111), K170 (≠ A164), M265 (≠ F256), V292 (= V285), V399 (≠ N393), G425 (≠ A419), M427 (= M421), D452 (= D446), N479 (= N473), H481 (≠ M475), L482 (= L476), M484 (= M478), V485 (= V479), W488 (vs. gap), H557 (≠ P544)
- binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (≠ E283), R291 (≠ F284), W488 (vs. gap)
- binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R154), G221 (= G210), G222 (= G212), G223 (≠ T213), T245 (≠ S236), L246 (≠ P237), M247 (≠ K238), L263 (= L254), G264 (= G255), M265 (≠ F256), H266 (≠ A257), G285 (= G278), R287 (≠ S280), D289 (≠ N282), R291 (≠ F284), D309 (= D303), I310 (= I304), G327 (= G321), D328 (= D322), V329 (≠ A323), M404 (≠ A398), G422 (≠ L416)
- binding magnesium ion: D452 (= D446), N479 (= N473), H481 (≠ M475)
- binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ N393), G400 (= G394), Q401 (≠ N395), H402 (≠ S396), M427 (= M421), G451 (= G445), D452 (= D446), G453 (= G447), S454 (= S448), N479 (= N473), H481 (≠ M475), L482 (= L476), G483 (= G477), M484 (= M478), V485 (= V479)
Sites not aligning to the query:
3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 14:559/582 of 3e9yA
- active site: Y32 (≠ V22), G34 (= G24), G35 (= G25), A36 (= A26), S37 (≠ I27), E58 (= E47), T81 (= T70), F120 (≠ I109), Q121 (= Q110), E122 (= E111), K170 (≠ A164), M265 (≠ F256), V292 (= V285), V399 (≠ N393), G425 (≠ A419), M427 (= M421), D452 (= D446), N479 (= N473), H481 (≠ M475), L482 (= L476), M484 (= M478), V485 (= V479), W488 (vs. gap), H557 (≠ P544)
- binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (≠ E283), R291 (≠ F284), W488 (vs. gap)
- binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R154), G221 (= G210), G222 (= G212), G223 (≠ T213), T245 (≠ S236), L246 (≠ P237), M247 (≠ K238), L263 (= L254), G285 (= G278), R287 (≠ S280), D289 (≠ N282), R291 (≠ F284), D309 (= D303), I310 (= I304), G327 (= G321), D328 (= D322), V329 (≠ A323), M404 (≠ A398), G422 (≠ L416)
- binding magnesium ion: D452 (= D446), N479 (= N473), H481 (≠ M475)
- binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ N393), G400 (= G394), Q401 (≠ N395), H402 (≠ S396), M427 (= M421), G451 (= G445), G453 (= G447), S454 (= S448), N479 (= N473), H481 (≠ M475), L482 (= L476), G483 (= G477), M484 (= M478), V485 (= V479)
Sites not aligning to the query:
7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 15:560/582 of 7tzzA
- binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (≠ F256), R292 (≠ F284), W489 (vs. gap)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ N393), G401 (= G394), Q402 (≠ N395), H403 (≠ S396), G426 (≠ A419), M428 (= M421), G452 (= G445), D453 (= D446), G454 (= G447), S455 (= S448), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
- binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G210), G223 (= G212), G224 (≠ T213), T246 (≠ S236), L247 (≠ P237), M248 (≠ K238), M263 (≠ V253), L264 (= L254), M266 (≠ F256), H267 (≠ A257), G286 (= G278), R288 (≠ S280), V293 (= V285), D310 (= D303), I311 (= I304), D329 (= D322), V330 (≠ A323), M405 (≠ A398), G423 (≠ L416)
- binding magnesium ion: A37 (= A26), T82 (= T70), S83 (≠ A71), Q122 (= Q110), Y381 (≠ R374), D453 (= D446), M458 (= M451), Q461 (≠ M454), N480 (= N473), H482 (≠ M475), K533 (≠ P518)
Sites not aligning to the query:
6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl (see paper)
31% identity, 98% coverage: 1:548/562 of query aligns to 12:560/597 of 6demA
- active site: Y33 (≠ V22), G35 (= G24), G36 (= G25), A37 (= A26), I38 (= I27), E59 (= E47), T82 (= T70), F121 (≠ I109), Q122 (= Q110), E123 (= E111), K171 (≠ A164), K228 (≠ E221), M264 (≠ F256), V291 (= V285), V407 (≠ N393), L432 (≠ F418), G433 (≠ A419), M435 (= M421), D460 (= D446), N487 (= N473), E489 (≠ M475), Q490 (≠ L476), M492 (= M478), V493 (= V479), W496 (≠ G482), L518 (= L504), N523 (≠ G509), V524 (≠ A510)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: M264 (≠ F256), D289 (≠ E283), R290 (≠ F284), M492 (= M478), W496 (≠ G482)
- binding flavin-adenine dinucleotide: R161 (= R154), G217 (= G210), A218 (≠ W211), G219 (= G212), N222 (≠ L215), T244 (≠ S236), L245 (≠ P237), Q246 (≠ K238), L262 (= L254), G284 (= G278), A285 (≠ T279), R286 (≠ S280), D288 (≠ N282), R290 (≠ F284), V291 (= V285), E317 (≠ D303), I318 (= I304), N322 (≠ E308), D336 (= D322), V337 (≠ A323), M412 (≠ A398), G430 (≠ L416)
- binding magnesium ion: D460 (= D446), N487 (= N473), E489 (≠ M475)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (≠ N393), G408 (= G394), Q409 (≠ N395), H410 (≠ S396), M435 (= M421), G459 (= G445), D460 (= D446), A461 (≠ G447), S462 (= S448), M465 (= M451), N487 (= N473), E489 (≠ M475), Q490 (≠ L476), G491 (= G477), M492 (= M478), V493 (= V479)
Sites not aligning to the query:
6delA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide chlorimuron ethyl (see paper)
31% identity, 98% coverage: 1:548/562 of query aligns to 12:560/597 of 6delA
- active site: Y33 (≠ V22), G35 (= G24), G36 (= G25), A37 (= A26), I38 (= I27), E59 (= E47), T82 (= T70), F121 (≠ I109), Q122 (= Q110), E123 (= E111), K171 (≠ A164), K228 (≠ E221), M264 (≠ F256), V291 (= V285), V407 (≠ N393), L432 (≠ F418), G433 (≠ A419), M435 (= M421), D460 (= D446), N487 (= N473), E489 (≠ M475), Q490 (≠ L476), M492 (= M478), V493 (= V479), W496 (≠ G482), L518 (= L504), N523 (≠ G509), V524 (≠ A510)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: D289 (≠ E283), R290 (≠ F284), W496 (≠ G482)
- binding flavin-adenine dinucleotide: R161 (= R154), G217 (= G210), A218 (≠ W211), G219 (= G212), N222 (≠ L215), T244 (≠ S236), L245 (≠ P237), Q246 (≠ K238), L262 (= L254), G284 (= G278), A285 (≠ T279), R286 (≠ S280), D288 (≠ N282), R290 (≠ F284), V291 (= V285), E317 (≠ D303), I318 (= I304), N322 (≠ E308), D336 (= D322), V337 (≠ A323), M412 (≠ A398), G430 (≠ L416)
- binding (3Z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl](formyl)amino}-3-sulfanylpent-3-en-1-yl trihydrogen diphosphate: V407 (≠ N393), G408 (= G394), Q409 (≠ N395), H410 (≠ S396), G433 (≠ A419), M435 (= M421), G459 (= G445), D460 (= D446), A461 (≠ G447), S462 (= S448), M465 (= M451), N487 (= N473), E489 (≠ M475), Q490 (≠ L476), G491 (= G477), M492 (= M478), V493 (= V479)
- binding magnesium ion: D460 (= D446), N487 (= N473), E489 (≠ M475)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (≠ N393), G408 (= G394), Q409 (≠ N395), H410 (≠ S396), G433 (≠ A419), M435 (= M421), G459 (= G445), D460 (= D446), A461 (≠ G447), S462 (= S448), M465 (= M451), N487 (= N473), E489 (≠ M475), Q490 (≠ L476), G491 (= G477), M492 (= M478), V493 (= V479)
5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 15:560/585 of 5k2oA
- active site: Y33 (≠ V22), G35 (= G24), G36 (= G25), A37 (= A26), S38 (≠ I27), E59 (= E47), T82 (= T70), F121 (≠ I109), Q122 (= Q110), E123 (= E111), K171 (≠ A164), M266 (≠ F256), V293 (= V285), V400 (≠ N393), G426 (≠ A419), M428 (= M421), D453 (= D446), N480 (= N473), H482 (≠ M475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (vs. gap), H558 (≠ P544)
- binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (≠ F256), R292 (≠ F284), W489 (vs. gap)
- binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G210), G223 (= G212), G224 (≠ T213), T246 (≠ S236), L247 (≠ P237), M248 (≠ K238), L264 (= L254), G286 (= G278), R288 (≠ S280), D290 (≠ N282), V293 (= V285), D310 (= D303), I311 (= I304), D329 (= D322), V330 (≠ A323), Q404 (≠ M397), M405 (≠ A398), G423 (≠ L416)
- binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ M475)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ N393), G401 (= G394), Q402 (≠ N395), H403 (≠ S396), M428 (= M421), D453 (= D446), G454 (= G447), S455 (= S448), N480 (= N473), H482 (≠ M475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
Sites not aligning to the query:
P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
32% identity, 97% coverage: 4:546/562 of query aligns to 100:645/670 of P17597
- A122 (= A26) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
- M124 (≠ E28) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
- E144 (= E47) binding thiamine diphosphate
- S186 (= S89) binding FAD
- P197 (≠ S100) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
- R199 (≠ S102) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
- Q207 (= Q110) binding thiamine diphosphate
- K220 (= K128) binding (R)-imazaquin
- R246 (= R154) binding (R)-imazaquin; binding FAD
- K256 (≠ A164) binding chlorimuron-ethyl
- G308 (= G212) binding FAD
- TL 331:332 (≠ SP 236:237) binding FAD
- C340 (≠ E245) modified: Cysteine sulfinic acid (-SO2H)
- LGMH 349:352 (≠ LGFA 254:257) binding FAD
- GVRFD 371:375 (≠ GTSFN 278:282) binding FAD
- DR 376:377 (≠ EF 283:284) binding chlorimuron-ethyl
- DI 395:396 (= DI 303:304) binding FAD
- DV 414:415 (≠ DA 322:323) binding FAD
- QH 487:488 (≠ NS 395:396) binding thiamine diphosphate
- GG 508:509 (≠ LG 416:417) binding FAD
- GAM 511:513 (≠ ASM 419:421) binding thiamine diphosphate
- D538 (= D446) binding Mg(2+)
- DGS 538:540 (= DGS 446:448) binding thiamine diphosphate
- N565 (= N473) binding Mg(2+)
- NQHLGM 565:570 (≠ NAMLGM 473:478) binding thiamine diphosphate
- H567 (≠ M475) binding Mg(2+)
- W574 (vs. gap) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
Sites not aligning to the query:
- 653 binding chlorimuron-ethyl; S→A: No effect on catalytic activity or sensitivity to herbicides.; S→F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; S→N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; S→T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 15:560/582 of 8et4A
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ N393), G401 (= G394), Q402 (≠ N395), H403 (≠ S396), G426 (≠ A419), M428 (= M421), G452 (= G445), D453 (= D446), G454 (= G447), S455 (= S448), M458 (= M451), N480 (= N473), H482 (≠ M475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
- binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G210), G223 (= G212), G224 (≠ T213), T246 (≠ S236), L247 (≠ P237), M248 (≠ K238), L264 (= L254), M266 (≠ F256), H267 (≠ A257), G286 (= G278), V287 (≠ T279), R288 (≠ S280), D290 (≠ N282), R292 (≠ F284), V293 (= V285), D310 (= D303), I311 (= I304), D329 (= D322), V330 (≠ A323), M405 (≠ A398), G423 (≠ L416)
- binding magnesium ion: F370 (≠ T362), D453 (= D446), M458 (= M451), Q461 (≠ M454), N480 (= N473), H482 (≠ M475), K533 (≠ P518)
- binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (≠ F256), R292 (≠ F284), M485 (= M478), W489 (vs. gap)
Sites not aligning to the query:
5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 15:560/582 of 5wj1A
- active site: Y33 (≠ V22), G35 (= G24), G36 (= G25), A37 (= A26), S38 (≠ I27), E59 (= E47), T82 (= T70), F121 (≠ I109), Q122 (= Q110), E123 (= E111), K171 (≠ A164), M266 (≠ F256), V293 (= V285), V400 (≠ N393), G426 (≠ A419), M428 (= M421), D453 (= D446), N480 (= N473), H482 (≠ M475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (vs. gap), H558 (≠ P544)
- binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G210), G223 (= G212), G224 (≠ T213), T246 (≠ S236), L247 (≠ P237), M248 (≠ K238), M263 (≠ V253), L264 (= L254), G286 (= G278), R288 (≠ S280), V293 (= V285), D310 (= D303), I311 (= I304), D329 (= D322), V330 (≠ A323), M405 (≠ A398), G423 (≠ L416), G424 (= G417)
- binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ M475)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (≠ F256), D291 (≠ E283), R292 (≠ F284), M485 (= M478), W489 (vs. gap)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ N393), G401 (= G394), Q402 (≠ N395), H403 (≠ S396), M428 (= M421), D453 (= D446), G454 (= G447), S455 (= S448), M458 (= M451), N480 (= N473), H482 (≠ M475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
Sites not aligning to the query:
5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 15:560/582 of 5k6tA
- active site: Y33 (≠ V22), G35 (= G24), G36 (= G25), A37 (= A26), S38 (≠ I27), E59 (= E47), T82 (= T70), F121 (≠ I109), Q122 (= Q110), E123 (= E111), K171 (≠ A164), M266 (≠ F256), V293 (= V285), V400 (≠ N393), G426 (≠ A419), M428 (= M421), D453 (= D446), N480 (= N473), H482 (≠ M475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (vs. gap), H558 (≠ P544)
- binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (≠ A257), R292 (≠ F284), M485 (= M478), W489 (vs. gap)
- binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G210), G223 (= G212), G224 (≠ T213), T246 (≠ S236), L247 (≠ P237), M248 (≠ K238), L264 (= L254), G286 (= G278), R288 (≠ S280), D290 (≠ N282), R292 (≠ F284), V293 (= V285), D310 (= D303), I311 (= I304), D329 (= D322), V330 (≠ A323), Q404 (≠ M397), M405 (≠ A398), G423 (≠ L416)
- binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ M475)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ N393), G401 (= G394), Q402 (≠ N395), H403 (≠ S396), G426 (≠ A419), M428 (= M421), G452 (= G445), G454 (= G447), S455 (= S448), N480 (= N473), H482 (≠ M475), L483 (= L476), G484 (= G477)
Sites not aligning to the query:
5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 15:560/582 of 5k6rA
- active site: Y33 (≠ V22), G35 (= G24), G36 (= G25), A37 (= A26), S38 (≠ I27), E59 (= E47), T82 (= T70), F121 (≠ I109), Q122 (= Q110), E123 (= E111), K171 (≠ A164), M266 (≠ F256), V293 (= V285), V400 (≠ N393), G426 (≠ A419), M428 (= M421), D453 (= D446), N480 (= N473), H482 (≠ M475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (vs. gap), H558 (≠ P544)
- binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (≠ F284), W489 (vs. gap)
- binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G210), G223 (= G212), G224 (≠ T213), T246 (≠ S236), L247 (≠ P237), M248 (≠ K238), L264 (= L254), M266 (≠ F256), G286 (= G278), R288 (≠ S280), R292 (≠ F284), V293 (= V285), D310 (= D303), I311 (= I304), G328 (= G321), D329 (= D322), V330 (≠ A323), M405 (≠ A398), G423 (≠ L416)
- binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ M475)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ N393), G401 (= G394), Q402 (≠ N395), H403 (≠ S396), G426 (≠ A419), M428 (= M421), D453 (= D446), G454 (= G447), S455 (= S448), M458 (= M451), N480 (= N473), H482 (≠ M475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
Sites not aligning to the query:
5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 15:560/583 of 5k3sA
- active site: Y33 (≠ V22), G35 (= G24), G36 (= G25), A37 (= A26), S38 (≠ I27), E59 (= E47), T82 (= T70), F121 (≠ I109), Q122 (= Q110), E123 (= E111), K171 (≠ A164), M266 (≠ F256), V293 (= V285), V400 (≠ N393), G426 (≠ A419), M428 (= M421), D453 (= D446), N480 (= N473), H482 (≠ M475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (vs. gap), H558 (≠ P544)
- binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (≠ F284), M485 (= M478), W489 (vs. gap)
- binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G210), G223 (= G212), G224 (≠ T213), T246 (≠ S236), L247 (≠ P237), M248 (≠ K238), L264 (= L254), M266 (≠ F256), G286 (= G278), R288 (≠ S280), D290 (≠ N282), V293 (= V285), D310 (= D303), I311 (= I304), D329 (= D322), V330 (≠ A323), M405 (≠ A398), G423 (≠ L416)
- binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ M475)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ N393), G401 (= G394), Q402 (≠ N395), H403 (≠ S396), G426 (≠ A419), M428 (= M421), D453 (= D446), G454 (= G447), S455 (= S448), N480 (= N473), H482 (≠ M475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
Sites not aligning to the query:
1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 15:560/582 of 1z8nA
- active site: Y33 (≠ V22), G35 (= G24), G36 (= G25), A37 (= A26), S38 (≠ I27), E59 (= E47), T82 (= T70), F121 (≠ I109), Q122 (= Q110), E123 (= E111), K171 (≠ A164), M266 (≠ F256), V293 (= V285), V400 (≠ N393), G426 (≠ A419), M428 (= M421), D453 (= D446), N480 (= N473), H482 (≠ M475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (vs. gap), H558 (≠ P544)
- binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K128), R161 (= R154), Y191 (≠ F187), R194 (vs. gap), D291 (≠ E283), R292 (≠ F284), D312 (= D305), W489 (vs. gap)
- binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G210), G224 (≠ T213), T246 (≠ S236), L247 (≠ P237), M248 (≠ K238), L264 (= L254), G265 (= G255), M266 (≠ F256), H267 (≠ A257), G286 (= G278), V287 (≠ T279), R288 (≠ S280), D290 (≠ N282), R292 (≠ F284), V293 (= V285), D310 (= D303), I311 (= I304), D329 (= D322), V330 (≠ A323), M405 (≠ A398), G423 (≠ L416), G424 (= G417)
- binding magnesium ion: D453 (= D446), N480 (= N473)
- binding thiamine diphosphate: V400 (≠ N393), G401 (= G394), Q402 (≠ N395), H403 (≠ S396), G426 (≠ A419), M428 (= M421), G452 (= G445), G454 (= G447), S455 (= S448), N480 (= N473), H482 (≠ M475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
Sites not aligning to the query:
1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 15:560/582 of 1yi1A
- active site: Y33 (≠ V22), G35 (= G24), G36 (= G25), A37 (= A26), S38 (≠ I27), E59 (= E47), T82 (= T70), F121 (≠ I109), Q122 (= Q110), E123 (= E111), K171 (≠ A164), M266 (≠ F256), V293 (= V285), V400 (≠ N393), G426 (≠ A419), M428 (= M421), D453 (= D446), N480 (= N473), H482 (≠ M475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (vs. gap), H558 (≠ P544)
- binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (≠ E283), R292 (≠ F284), W489 (vs. gap)
- binding flavin-adenine dinucleotide: R161 (= R154), G223 (= G212), G224 (≠ T213), T246 (≠ S236), L247 (≠ P237), M248 (≠ K238), M263 (≠ V253), L264 (= L254), G265 (= G255), M266 (≠ F256), H267 (≠ A257), G286 (= G278), V287 (≠ T279), R288 (≠ S280), D290 (≠ N282), V293 (= V285), D310 (= D303), I311 (= I304), D329 (= D322), V330 (≠ A323), M405 (≠ A398), G423 (≠ L416), G424 (= G417)
- binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ M475)
Sites not aligning to the query:
1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 15:560/582 of 1yi0A
- active site: Y33 (≠ V22), G35 (= G24), G36 (= G25), A37 (= A26), S38 (≠ I27), E59 (= E47), T82 (= T70), F121 (≠ I109), Q122 (= Q110), E123 (= E111), K171 (≠ A164), M266 (≠ F256), V293 (= V285), V400 (≠ N393), G426 (≠ A419), M428 (= M421), D453 (= D446), N480 (= N473), H482 (≠ M475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (vs. gap), H558 (≠ P544)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ E283), R292 (≠ F284), W489 (vs. gap)
- binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G210), G223 (= G212), G224 (≠ T213), T246 (≠ S236), L247 (≠ P237), M248 (≠ K238), L264 (= L254), G265 (= G255), M266 (≠ F256), H267 (≠ A257), G286 (= G278), V287 (≠ T279), R288 (≠ S280), D290 (≠ N282), R292 (≠ F284), V293 (= V285), D310 (= D303), I311 (= I304), G328 (= G321), D329 (= D322), V330 (≠ A323), M405 (≠ A398), G423 (≠ L416), G424 (= G417)
- binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ M475)
Sites not aligning to the query:
1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
32% identity, 97% coverage: 4:546/562 of query aligns to 15:560/582 of 1yhzA
- active site: Y33 (≠ V22), G35 (= G24), G36 (= G25), A37 (= A26), S38 (≠ I27), E59 (= E47), T82 (= T70), F121 (≠ I109), Q122 (= Q110), E123 (= E111), K171 (≠ A164), M266 (≠ F256), V293 (= V285), V400 (≠ N393), G426 (≠ A419), M428 (= M421), D453 (= D446), N480 (= N473), H482 (≠ M475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (vs. gap), H558 (≠ P544)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (≠ E283), R292 (≠ F284), M485 (= M478), W489 (vs. gap)
- binding flavin-adenine dinucleotide: R161 (= R154), G223 (= G212), G224 (≠ T213), T246 (≠ S236), L247 (≠ P237), M248 (≠ K238), L264 (= L254), M266 (≠ F256), H267 (≠ A257), G286 (= G278), V287 (≠ T279), R288 (≠ S280), D290 (≠ N282), V293 (= V285), D310 (= D303), I311 (= I304), D329 (= D322), V330 (≠ A323), Q404 (≠ M397), M405 (≠ A398), G423 (≠ L416), G424 (= G417)
- binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ M475)
Sites not aligning to the query:
Query Sequence
>WP_012469185.1 NCBI__GCF_000020385.1:WP_012469185.1
MNAAEVIVRSLEKHGVTCVFGVPGGAIESLNTALYKSSISVIVAKHEEGAAFMADGYARV
SGVIAVCCSTAGPGATNMITGIASAFGDSIPVIALTGQVSTSLFGKGAIQEFSVQSFGIV
SIFRQITKYSDIIINENKAGEMISRARRLALTGRKGPVHLNLPADIMKRKVTDDSHERCS
TTQVLGFDREAVKKAAQLLLGAKRPVIVAGWGTVLSRADKELLELAELLDIPVATSPKAK
GILSEVHPLSLGVLGFAGSPVAKEYILKRDVDVLLAVGTSFNEFVTSGWDKRLLPMKSLI
HVDIDCNEIGKNYYVHVGIAGDAATVLRELVFEIGRIRKGRRIENTRRKEVEQVRAALLV
KTRKQNKALYSPHRLIMDLNEALPKNTLYFADNGNSMAWAIRYLTITEPYSFYVGLGFAS
MGFAVAAPIGAKLAAGDRPVVALVGDGSFLMNGMEVATAVNYNIPVIWVIMNNAMLGMVY
HGRKLAGIPEGIPSGFKPVDHVKLAEALGARGIRITTPGEINRELMDDILAAGVPTVLDV
IIDPEEVPPIHSRISSLEKLYT
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory