SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012469284.1 NCBI__GCF_000020385.1:WP_012469284.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
43% identity, 94% coverage: 22:435/439 of query aligns to 22:431/435 of Q5F8J4

query
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Q5F8J4

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L45 (= L45) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
L--S 45:46 (≠ LDIT 45:48) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
39% identity, 98% coverage: 4:435/439 of query aligns to 5:429/431 of 6dzsA

query
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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G10), L12 (≠ F11), G13 (= G12), N14 (= N13), V15 (≠ I14), V45 (≠ D44), R46 (≠ L45), R47 (≠ D46), R52 (= R51), I63 (≠ V64), L78 (= L79), M79 (≠ I80), P84 (= P85), A102 (= A103), K104 (= K105), G306 (= G303), T310 (= T307)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
39% identity, 73% coverage: 1:319/439 of query aligns to 2:307/402 of 4pg7A

query
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4pg7A

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active site: D190 (= D201), K194 (= K205)
binding lysine: R215 (≠ E226), G219 (≠ A230)

Sites not aligning to the query:

binding lysine: 394, 395

F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
34% identity, 72% coverage: 4:320/439 of query aligns to 1:301/304 of F9VNG5

query
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F9VNG5

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F

E

K

I

A

F

F

N

E

D

K

P

I

E

K

I

P

S

D

I

I

V

I

I

V

E

D

L

V

I
x
S

G

S

G

A

Y

N

-

Y

-

N

-

G

E

E

P

P

A

S

K

L

T

S

F

L

V

Y

L

K

K

E

A

A

I

I

E

K

A

D

G

G

K

V

H

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

L

L

H

A

G

F

D

N

E

E

I

I

Y

F

A

S

A

L

A

A

A

R

R

S

K

K

G

G

V

V

E

K

V

I

Q

G

F

F

E

Q

A

G

A

T

V

V

G

M

G

S

G

G

I

T

P

P

V

S

L

I

S

N

A

L

I

Y

K

R

G

V

N

-

L

L

S

P

G

G

N

S

R

R

F

V

S

I

S

K

V

I

F

R

G

G

I

I

M

L

N

N

G

G

T

T

C

T

N

N

Y

F

I

I

L

L

T

T

R

L

M

M

T

N

Q

K

E

-

G

G

A

V

D

S

F

F

E

E

D

E

V

A

L

L

K

K

S

E

A

A

M

Q

E

R

L

R

G
|
G

Y

Y

A

A

E
|
E

P

E

D

D

P

P

T

T

F

L

D

D

I

I

E

N

G

G

V

F

D

D

T

A

A

A

H

A

K

K

L

I

A

T

V

I

L

L

A

A

S

N

L

F

C

M

F

I

G

G

T

N

K

S

I

V

D

T

F

I

N

K

A

D

I

V

H

K

T

F

E

E

G

G

I

I

T

N

A

R

I

-

S

-

G

-

I

-

D

D

I

L

R

P

F

K

A

N

E

E

Q

-

F

-

G

-

Y

-

R

K

I

I

K

K

L

L

L

I

A

A

I

-

G

-

K

-

L

Y

T

A

D

D

D

E

R

K

Q

-

L

-

E

E

V

V

R

W

V

V

H

K

P

P

T

L

M

P

I

I

P

S

L

Q

R

D

N

D

P

P

L

L

A

Y

D

N

V

V

N

D

G

G

V

V

F

E

N

N

A

A

I

L

H

E

L

I

T

T

G

T

D

D

F

-

V

I

G

Q

P

S

V

I

L

L

F

I

Y

R

G

G

R

P

G

G

A

A

G
|
G

Q

P

N

V

P

N

T

A

A

A

S

Y

A

G

I

A

V

L

G

S

D

D

L

L

V

I

A

L

L

L

S

K

R

R

Y8 (≠ F11) binding NADP(+)
N10 (= N13) binding NADP(+)
V11 (≠ I14) binding NADP(+)
R38 (≠ K40) binding NADP(+)
R39 (= R41) binding NADP(+)
S73 (≠ I80) binding NADP(+)
T100 (≠ A103) binding NADP(+)
K102 (= K105) binding NADP(+)
G182 (= G187) binding NADP(+)
E185 (= E190) binding NADP(+)
G284 (= G303) binding NADP(+)

Sites not aligning to the query:

303 modified: Interchain

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
34% identity, 72% coverage: 4:320/439 of query aligns to 1:297/300 of 7f4cA

query
sites
7f4cA

I

M

K

K

V

L

G

L

L

L

I
x
F

G
|
G

F
x
Y

G
|
G

N
|
N

I
x
V

G

G

A

K

G

A

V

F

V

R

R

K

L

L

Q

H

E

E

N

K

-

R

A

S

Q

P

T

E

V

L

Q

N

Q

D

K

V

V

I

G

I

S

G

G

G

I

I

I

V

L
x
T

K
x
R

R
|
R

I

G

A

I

D

M

L

L

-

Q

-

D

-

K

-

E

D

D

I

F

T

T

S

P

D

D

R

-

G

-

V

-

S

-

V

-

D

-

P

-

A

-

M

-

L

L

T

E

T

G

D

D

V

V

N

F

E

K

I

A

F

F

N

E

D

K

P

I

E

K

I

P

S

D

I

I

V

I

I

V

E

D

L
x
V

I
x
S

G
x
S

G

A

Y

N

-

Y

-

N

-

G

E

E

P

P

A

S

K

L

T

S

F

L

V

Y

L

K

K

E

A

A

I

I

E

K

A

D

G

G

K

V

H

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

L

L

H

A

G

F

D

N

E

E

I

I

Y

F

A

S

A

L

A

A

A

R

R

S

K

K

G

G

V

V

E

K

V

I

Q

G

F

F

E

Q

A
x
G

A

T

V

V

G

M

G
x
S

G

G

I

T

P

P

V

S

L

I

S

N

A

L

I

Y

K

R

G

V

N

-

L

L

S

P

G

G

N

S

R

R

F

V

S

I

S

K

V

I

F

R

G

G

I

I

M

L

N

N

G

G

T

T

C

T

N

N

Y

F

I

I

L

L

T

T

R

L

M

M

T

N

Q

K

E

-

G

G

A

V

D

S

F

F

E

E

D

E

V

A

L

L

K

K

S

E

A

A

M

Q

E

R

L

R

G

G

Y

Y

A

A

E
|
E

P

E

D

D

P

P

T

T

F

L

D

D

I

I

E

N

G

G

V

F

D

D

T

A

A

A

H

A

K

K

L

I

A

T

V

I

L

L

A

A

S

N

L

F

C

M

F

I

G

G

T

N

K

S

I

V

D

T

F

I

N

K

A

D

I

V

H

K

T

F

E

E

G

G

I

I

T

N

A

R

I

-

S

-

G

-

I

-

D

-

I

-

R

-

F

-

A

-

E

-

Q

-

F

-

G

D

Y

L

R

K

I

I

K

K

L

L

L

I

A

A

I

-

G

-

K

-

L

Y

T

A

D

D

D

E

R

K

Q

-

L

-

E

E

V

V

R

W

V

V

H

K

P

P

T

L

M

P

I

I

P

S

L

Q

R

D

N

D

P

P

L

L

A

Y

D

N

V

V

N

D

G

G

V

V

F

E

N

N

A

A

I

L

H

E

L

I

T

T

G

T

D

D

F

-

V

I

G

Q

P

S

V

I

L

L

F

I

Y

R

G

G

R

P

G

G

A

A

G
|
G

Q

P

N

V

P

N

T
x
A

A

A

S

Y

A

G

I

A

V

L

G

S

D

D

L

L

V

I

A

L

L

L

S

K

R

R

binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ I9), G7 (= G10), Y8 (≠ F11), G9 (= G12), N10 (= N13), V11 (≠ I14), T37 (≠ L39), R38 (≠ K40), R39 (= R41), V72 (≠ L79), S73 (≠ I80), S74 (≠ G81), T100 (≠ A103), K102 (= K105), G127 (≠ A130), S131 (≠ G134), E185 (= E190), G280 (= G303), A284 (≠ T307)

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
33% identity, 72% coverage: 4:320/439 of query aligns to 1:299/302 of 5x9dA

query
sites
5x9dA

I

M

K

K

V

L

G

L

L

L

I
x
F

G
|
G

F
x
Y

G
|
G

N
|
N

I
x
V

G

G

A

K

G

A

V

F

V

R

R

K

L

L

Q

H

E

E

N

K

-

R

A

S

Q

P

T

E

V

L

Q

N

Q

D

K

V

V

I

G

I

S

G

G

G

I

I

I

V

L
x
T

K
x
R

R

R

I

G

A

I

D

M

L

L

-

Q

-

D

-

K

-

E

D

D

I

F

T

T

S

P

D

D

R

-

G

-

V

-

S

-

V

-

D

-

P

-

A

-

M

-

L

L

T

E

T

G

D

D

V

V

N

F

E

K

I

A

F

F

N

E

D

K

P

I

E

K

I

P

S

D

I

I

V

I

I

V

E

D

L
x
V

I
x
S

G
x
S

G

A

Y

N

-

Y

-

N

-

G

E

E

P
|
P

A

S

K

L

T

S

F

L

V

Y

L

K

K

E

A

A

I

I

E

K

A

D

G

G

K

V

H

N

V

I

T

T

A
x
T

N
|
N

K
|
K

A

A

L

P

L

L

A

A

L

L

H

A

G

F

D

N

E

E

I

I

Y

F

A

S

A

L

A

A

A

R

R

S

K

K

G

G

V

V

E

K

V

I

Q

G

F

F

E

Q

A
x
G

A

T

V

V

G

M

G
x
S

G

G

I

T

P

P

V

S

L

I

S

N

A

L

I

Y

K

R

G

V

N

-

L

L

S

P

G

G

N

S

R

R

F

V

S

I

S

K

V

I

F

R

G

G

I

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

N

Y

F

I

I

L

L

T

T

R

L

M

M

T

N

Q

K

E

-

G

G

A

V

D

S

F

F

E

E

D

E

V

A

L

L

K

K

S

E

A

A

M

Q

E

R

L

R

G

G

Y
|
Y

A
|
A

E
|
E

P

E

D

D

P

P

T

T

F

L

D
|
D

I

I

E

N

G

G

V

F

D
|
D

T

A

A

A

H

A

K
|
K

L

I

A

T

V

I

L

L

A

A

S

N

L

F

C

M

F

I

G

G

T

N

K

S

I

V

D

T

F

I

N

K

A

D

I

V

H

K

T

F

E

E

G

G

I

I

T

N

A

-

I

-

S

-

G

-

I

-

D

-

I

-

R

-

F

-

A

R

E

D

Q

P

F

K

G

N

Y

E

R

K

I

I

K

K

L

L

L

I

A

A

I

-

G

-

K

-

L

Y

T

A

D

D

D

E

R

K

Q

E

L

V

E

W

V

V

R

K

V

P

H

L

P

P

T

I

M

S

I

-

P

-

L

-

R

Q

N

D

P

P

L

L

A

Y

D

N

V

V

N

D

G

G

V

V

F

E

N

N

A

A

I

L

H

E

L

I

T

T

G

T

D

D

F

-

V

I

G

Q

P

S

V

I

L

L

F

I

Y

R

G

G

R

P

G

G

A
|
A

G
|
G

Q

P

N

V

P

N

T
x
A

A

A

S

Y

A

G

I

A

V

L

G

S

D

D

L

L

V

I

A

L

L

L

S

K

R

R

active site: D196 (= D201), K200 (= K205)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ I9), G7 (= G10), Y8 (≠ F11), G9 (= G12), N10 (= N13), V11 (≠ I14), T37 (≠ L39), R38 (≠ K40), V72 (≠ L79), S73 (≠ I80), S74 (≠ G81), P82 (= P85), T100 (≠ A103), N101 (= N104), K102 (= K105), G127 (≠ A130), S131 (≠ G134), N155 (= N159), G156 (= G160), T157 (= T161), Y183 (= Y188), A184 (= A189), E185 (= E190), D191 (= D196), D196 (= D201), K200 (= K205), A281 (= A302), G282 (= G303), A286 (≠ T307)

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
33% identity, 67% coverage: 1:293/439 of query aligns to 1:280/332 of 6a0tB

query
sites
6a0tB

M

M

N

E

E

A

I

L

K

K

V

I

G

A

L

L

I

L

G
|
G

F
x
G

G
|
G

N
x
T

I
x
V

G

G

A

S

G

A

V

F

V

Y

R

N

L

L

L

V

Q

L

E

E

N

R

A

A

Q

E

T

E

V

L

Q

S

Q

-

K

-

V

-

G

A

S

F

G

G

I

V

L

-

K

P

R

R

I

F

A

L

D

G

L

V

D
x
L

I
x
V

T
x
R

S
x
D

D

P

R

R

G
x
K

V

P

S
x
R

V

A

D

I

P

P

A

Q

M

E

L

L

T

L

T

R

D

-

V

-

N

A

E

E

I

P

F

F

N

D

D

L

P

L

E

E

I

A

S

D

I

L

V

V

I

V

E

E

L
x
A

I
x
M

G
|
G

G

G

Y

V

E

E

P

A

A

P

K

L

T

R

F

L

V

V

L

L

K

P

A

A

I

L

E

E

A

A

G

G

K

I

H

P

V

L

V

I

T

T

A
|
A

N
|
N

K

K

A

A

L

L

A

A

L

-

H

E

G

A

D

W

E

E

I

S

Y

L

A

R

A

P

A

F

A

A

R

E

K

E

G

G

V

L

E

-

V

I

Q

Y

F

H

E

E

A

A

A

S

V

V

G

M

G

A

G

G

I

T

P

P

V

A

L

L

S

S

A

F

I

L

K

E

G

-

N

T

L

L

S

R

G

G

N

S

R

E

F

L

S

L

S

E

V

L

F

H

G

G

I

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

I

I

L

L

T

Q

R

E

M

M

T

-

Q

E

E

K

G

G

A

R

D

T

F

Y

E

A

D

E

V

A

L

L

K

L

S

E

A

A

M

Q

E

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

P

A

D

D

P

P

T

T

F

L

D
|
D

I

V

E

E

G

G

V

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

A

T

V

L

L

L

A

A

S

R

L

L

C

L

F

V

G

D

T

P

K

G

I

F

D

P

F

F

N

A

A

E

I

V

H

E

T

A

E

Q

G

G

I

I

T

A

A

R

I

L

S

T

G

P

I

E

D

V

I

L

R

Q

F

K

A

A

E

E

Q

A

F

R

G

G

Y

E

R

R

I

V

K

R

L

L

L

V

A

A

I

-

G

S

K

L

L

F

T

G

D

E

D

G

R

G

Q

R

L

W

E

R

V

A

R

A

V

V

H

A

P

P

T

R

M

R

I

L

P

P

L

Q

R

D

N

H

P

P

L

L

A

A

D

R

V

A

N

R

G

G

V

-

F

-

N

N

A

A

I

L

H

W

L

V

T

R

G

A

D

R

F

P

V

L

G

G

active site: D191 (= D201), K195 (= K205)
binding l-homoserine: N150 (= N159), G151 (= G160), T152 (= T161), Y178 (= Y188), E180 (= E190), D186 (= D196), K195 (= K205)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), G11 (≠ F11), G12 (= G12), T13 (≠ N13), V14 (≠ I14), L42 (≠ D46), V43 (≠ I47), R44 (≠ T48), D45 (≠ S49), K48 (≠ G52), R50 (≠ S54), A73 (≠ L79), M74 (≠ I80), G75 (= G81), A97 (= A103), N98 (= N104), G177 (= G187), E180 (= E190)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
33% identity, 67% coverage: 1:293/439 of query aligns to 1:280/331 of 6a0sA

query
sites
6a0sA

M

M

N

E

E

A

I

L

K

K

V

I

G

A

L

L

I

L

G

G

F
x
G

G
|
G

N
x
T

I
x
V

G

G

A

S

G

A

V

F

V

Y

R

N

L

L

L

V

Q

L

E

E

N

R

A

A

Q

E

T

E

V

L

Q

S

Q

-

K

-

V

-

G

A

S

F

G

G

I

V

L

-

K

P

R

R

I

F

A

L

D

G

L

V

D
x
L

I
x
V

T
x
R

S
x
D

D

P

R

R

G
x
K

V

P

S
x
R

V

A

D

I

P

P

A

Q

M

E

L

L

T

L

T

R

D

-

V

-

N

A

E

E

I

P

F

F

N

D

D

L

P

L

E

E

I

A

S

D

I

L

V

V

I

V

E

E

L
x
A

I
x
M

G

G

Y

V

E

E

P

A

A

P

K

L

T

R

F

L

V

V

L

L

K

P

A

A

I

L

E

E

A

A

G

G

K

I

H

P

V

L

V

I

T

T

A
|
A

N

N

K
|
K

A

A

L

L

A

A

L

-

H

E

G

A

D

W

E

E

I

S

Y

L

A

R

A

P

A

F

A

A

R

E

K

E

G

G

V

L

E

-

V

I

Q

Y

F

H

E

E

A

A

A

S

V

V

G

M

G

A

G

G

I

T

P

P

V

A

L

L

S

S

A

F

I

L

K

E

G

-

N

T

L

L

S

R

G

G

N

S

R

E

F

L

S

L

S

E

V

L

F

H

G

G

I

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

I

I

L

L

T

Q

R

E

M

M

T

-

Q

E

E

K

G

G

A

R

D

T

F

Y

E

A

D

E

V

A

L

L

K

L

S

E

A

A

M

Q

E

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

P

A

D

D

P

P

T

T

F

L

D
|
D

I

V

E

E

G

G

V

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

A

T

V

L

L

L

A

A

S

R

L

L

C

L

F

V

G

D

T

P

K

G

I

F

D

P

F

F

N

A

A

E

I

V

H

E

T

A

E

Q

G

G

I

I

T

A

A

R

I

L

S

T

G

P

I

E

D

V

I

L

R

Q

F

K

A

A

E

E

Q

A

F

R

G

G

Y

E

R

R

I

V

K

R

L

L

L

V

A

A

I

-

G

S

K

L

L

F

T

G

D

E

D

G

R

G

Q

R

L

W

E

R

V

A

R

A

V

V

H

A

P

P

T

R

M

R

I

L

P

P

L

Q

R

D

N

H

P

P

L

L

A

A

D

R

V

A

N

R

G

G

V

-

F

-

N

N

A

A

I

L

H

W

L

V

T

R

G

A

D

R

F

P

V

L

G

G

active site: D191 (= D201), K195 (= K205)
binding l-homoserine: K99 (= K105), N150 (= N159), G151 (= G160), T152 (= T161), Y178 (= Y188), E180 (= E190), D186 (= D196), K195 (= K205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ F11), G12 (= G12), T13 (≠ N13), V14 (≠ I14), L42 (≠ D46), V43 (≠ I47), R44 (≠ T48), D45 (≠ S49), K48 (≠ G52), R50 (≠ S54), A73 (≠ L79), M74 (≠ I80), A97 (= A103), K99 (= K105), G177 (= G187), E180 (= E190)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
33% identity, 67% coverage: 1:293/439 of query aligns to 1:280/331 of 2ejwA

query
sites
2ejwA

M

M

N

E

E

A

I

L

K

K

V

I

G

A

L

L

I

L

G

G

F

G

G

G

N

T

I

V

G

G

A

S

G

A

V

F

V

Y

R

N

L

L

L

V

Q

L

E

E

N

R

A

A

Q

E

T

E

V

L

Q

S

Q

-

K

-

V

-

G

A

S

F

G

G

I

V

L

-

K

P

R

R

I

F

A

L

D

G

L

V

D

L

I

V

T

R

S

D

D

P

R

R

G

K

V

P

S

R

V

A

D

I

P

P

A

Q

M

E

L

L

T

L

T

R

D

-

V

-

N

A

E

E

I

P

F

F

N

D

D

L

P

L

E

E

I

A

S

D

I

L

V

V

I

V

E

E

L

A

I

M

G

G

Y

V

E

E

P

A

A

P

K

L

T

R

F

L

V

V

L

L

K

P

A

A

I

L

E

E

A

A

G

G

K

I

H

P

V

L

V

I

T

T

A

A

N

N

K

K

A

A

L

L

A

A

L

-

H

E

G

A

D

W

E

E

I

S

Y

L

A

R

A

P

A

F

A

A

R

E

K

E

G

G

V

L

E

-

V

I

Q

Y

F

H

E
|
E

A

A

A

S

V
|
V

G
x
M

G
x
A

G

G

I
x
T

P

P

V

A

L

L

S

S

A

F

I

L

K

E

G

-

N

T

L

L

S

R

G

G

N

S

R

E

F

L

S

L

S

E

V

L

F

H

G

G

I

I

M

L

N

N

G

G

T

T

C

T

N

L

Y

Y

I

I

L

L

T

Q

R

E

M

M

T

-

Q

E

E

K

G

G

A

R

D

T

F

Y

E

A

D

E

V

A

L

L

K

L

S

E

A

A

M

Q

E

R

L

L

G

G

Y

Y

A

A

E

E

P

A

D

D

P

P

T

T

F

L

D

D

I

V

E

E

G

G

V

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

A

T

V

L

L

L

A

A

S

R

L

L

C

L

F

V

G

D

T

P

K

G

I

F

D

P

F

F

N

A

A

E

I

V

H

E

T

A

E

Q

G

G

I

I

T

A

A

R

I

L

S

T

G

P

I

E

D

V

I

L

R

Q

F

K

A

A

E

E

Q

A

F

R

G

G

Y

E

R

R

I

V

K

R

L

L

L

V

A

A

I

-

G

S

K

L

L

F

T

G

D

E

D

G

R

G

Q

R

L

W

E

R

V

A

R

A

V

V

H

A

P

P

T

R

M

R

I

L

P

P

L

Q

R

D

N

H

P

P

L

L

A

A

D

R

V

A

N

R

G

G

V

-

F

-

N

N

A

A

I

L

H

W

L

V

T

R

G

A

D

R

F

P

V

L

G

G

active site: D191 (= D201), K195 (= K205)
binding magnesium ion: E121 (= E129), V124 (= V132), M125 (≠ G133), A126 (≠ G134), T128 (≠ I136)

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
25% identity, 72% coverage: 3:316/439 of query aligns to 2:309/319 of 4xb2A

query
sites
4xb2A

E

K

I

V

K

N

V

I

G

S

L

I

I
x
F

G

G

F
|
F

G
|
G

N
x
T

I
x
V

G

G

A

R

G

A

V

L

V

A

R

E

L

I

Q

A

E

E

N

K

A

S

Q

R

T

I

V

F

Q

G

Q

V

K

E

V

L

G

N

S

V

G

I

I

S

I

I

L

T

K

D

R
|
R

-

S

-

G

-

T

-

I

I

W

A

G

D

D

L

F

D

D

I

L

T

L

S

E

D

A

R

K

G

E

V

V

S

A

V

E

D

S

P

T

A

G

M

K

L

L

T

S

T

N

D

I

V

G

N

D

-

Y

E

E

I

V

F

Y

N

N

D

F

P

S

E

P

I

Q

S

E

I

L

V

V

I

E

E

E

L

V

-

K

-

P

-

N

-

I

-

L

-

V

-

D

I
x
V

G
x
S

Y

W

E

D

P

E

A

A

K

H

T

E

F

M

V

Y

L

K

K

V

A

A

I

L

E

G

A

E

G

G

K

I

H

S

V

V

T

T

A
x
S

N
|
N

K
|
K

A

P

L

P

L

I

A

A

L

N

H

Y

G

Y

D

D

E

E

I

L

Y

M

A

N

A

L

A

A

A

K

R

E

K

N

G

N

V

A

E

G

V

I

Q

F

F

F

E

E

A
x
S

A

T

V

V

G

M

G

A

G

G

I

T

P

P

V

I

L

I

S

G

A

V

I

L

K

R

G

E

N

N

L

L

S

L

G

G

N

E

R

N

F

I

S

K

S

R

V

I

F

D

G

A

I

V

M

V

N

N

G
x
A

T
x
S

C

T

N

T

Y

F

I

I

L

L

T

T

R

K

M

M

T

S

Q

-

E

E

G

G

A

K

D

T

F

L

E

D

D

D

V

A

L

I

K

E

S

E

A

A

M

K

E

S

L

L

G

G

Y

I

A

L

E

E

P

E

D

D

P

P

T

S

F

K

D
|
D

I

I

E

D

G

G

V

I

D
|
D

T

A

A

Y

H

Y

K
|
K

L

A

A

K

V

I

L

L

A

H

S

W

L

V

C

S

F

Y

G

G

T

E

K

P

I

P

D

E

F

E

N

E

A

-

I

-

H

E

T

R

E

L

G

G

I

I

T

R

A

E

I

V

-

R

S

D

G

A

I

R

D

N

I

V

R

R

F

L

A

V

E

A

Q

Q

F

-

G

-

Y

-

R

-

I

-

K

-

L

-

L

V

A

S

I

K

G

G

K

K

L

I

T

S

D

-

R

-

Q

-

L

-

E

-

V

-

R

-

V

V

H

K

P

P

T

R

M

K

I

L

P

S

L

S

R

D

N

N

P

P

L

L

A

L

D

-

V

V

N

E

G

G

V

V

F

Q

N

N

A

A

I

A

H

V

L

I

T

R

G

T

D

N

F

N

V

L

G

G

P

E

V

V

L

I

F

L

Y

K

G

G

R

P

G

G

A
x
G

G

G

Q

G

N

R

P

V

T
|
T

A

A

S

S

A

G

I

V

V

F

G

T

D

D

L

I

V

I

active site: D211 (= D201), K215 (= K205)
binding l-homoserine: A171 (≠ G160), S172 (≠ T161), D206 (= D196), K215 (= K205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ I9), F10 (= F11), G11 (= G12), T12 (≠ N13), V13 (≠ I14), R40 (= R41), V91 (≠ I80), S92 (≠ G81), S93 (≠ G82), S114 (≠ A103), N115 (= N104), K116 (= K105), S141 (≠ A130), G295 (≠ A302), T300 (= T307)

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
25% identity, 72% coverage: 3:316/439 of query aligns to 2:309/319 of 4xb1A

query
sites
4xb1A

E

K

I

V

K

N

V

I

G

S

L

I

I
x
F

G

G

F
|
F

G
|
G

N
x
T

I
x
V

G

G

A

R

G

A

V

L

V

A

R

E

L

I

Q

A

E

E

N

K

A

S

Q

R

T

I

V

F

Q

G

Q

V

K

E

V

L

G

N

S

V

G

I

I

S

I

I

L

T

K
x
D

R
|
R

-

S

-

G

-

T

-

I

I

W

A

G

D

D

L

F

D

D

I

L

T

L

S

E

D

A

R

K

G

E

V

V

S
x
K

V

E

D

S

P

T

A

G

M

K

L

L

T

S

T

N

D

I

V

G

N

D

-

Y

E

E

I

V

F

Y

N

N

D

F

P

S

E

P

I

Q

S

E

I

L

V

V

I

E

E

E

L

V

-

K

-

P

-

N

-

I

-

L

-

V

-

D

I
x
V

G
x
S

Y

W

E

D

P

E

A

A

K

H

T

E

F

M

V

Y

L

K

K

V

A

A

I

L

E

G

A

E

G

G

K

I

H

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

L

N

H

Y

G

Y

D

D

E

E

I

L

Y

M

A

N

A

L

A

A

A

K

R

E

K

N

G

N

V

A

E

G

V

I

Q

F

F

F

E

E

A
x
S

A

T

V

V

G

M

G

A

G

G

I

T

P

P

V

I

L

I

S

G

A

V

I

L

K

R

G

E

N

N

L

L

S

L

G

G

N

E

R

N

F

I

S

K

S

R

V

I

F

D

G

A

I

V

M

V

N

N

G

A

T

S

C

T

N

T

Y

F

I

I

L

L

T

T

R

K

M

M

T

S

Q

-

E

E

G

G

A

K

D

T

F

L

E

D

D

D

V

A

L

I

K

E

S

E

A

A

M

K

E

S

L

L

G

G

Y

I

A

L

E

E

P

E

D

D

P

P

T

S

F

K

D

D

I

I

E

D

G

G

V

I

D
|
D

T

A

A

Y

H

Y

K
|
K

L

A

A

K

V

I

L

L

A

H

S

W

L

V

C

S

F

Y

G

G

T

E

K

P

I

P

D

E

F

E

N

E

A

-

I

-

H

E

T

R

E

L

G

G

I

I

T

R

A

E

I

V

-

R

S

D

G

A

I

R

D

N

I

V

R

R

F

L

A

V

E

A

Q

Q

F

-

G

-

Y

-

R

-

I

-

K

-

L

-

L

V

A

S

I

K

G

G

K

K

L

I

T

S

D

-

R

-

Q

-

L

-

E

-

V

-

R

-

V

V

H

K

P

P

T

R

M

K

I

L

P

S

L

S

R

D

N

N

P

P

L

L

A

L

D

-

V

V

N

E

G

G

V

V

F

Q

N

N

A

A

I

A

H

V

L

I

T

R

G

T

D

N

F

N

V

L

G

G

P

E

V

V

L

I

F

L

Y

K

G

G

R

P

G

G

A
x
G

G

G

Q

G

N

R

P

V

T
|
T

A

A

S

S

A

G

I

V

V

F

G

T

D

D

L

I

V

I

active site: D211 (= D201), K215 (= K205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ I9), F10 (= F11), G11 (= G12), T12 (≠ N13), V13 (≠ I14), D39 (≠ K40), R40 (= R41), K57 (≠ S54), V91 (≠ I80), S92 (≠ G81), S93 (≠ G82), S114 (≠ A103), K116 (= K105), S141 (≠ A130), G295 (≠ A302), T300 (= T307)

O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
25% identity, 72% coverage: 3:316/439 of query aligns to 2:309/319 of O58802

query
sites
O58802

E

K

I

V

K

N

V

I

G

S

L

I

I

F

G

G

F
|
F

G

G

N
x
T

I
x
V

G

G

A

R

G

A

V

L

V

A

R

E

L

I

Q

A

E

E

N

K

A

S

Q

R

T

I

V

F

Q

G

Q

V

K

E

V

L

G

N

S

V

G

I

I

S

I

I

L

T

K

D

R
|
R

-

S

-

G

-

T

-

I

I

W

A

G

D

D

L

F

D

D

I

L

T

L

S

E

D

A

R

K

G

E

V

V

S
x
K

V

E

D

S

P

T

A

G

M

K

L

L

T

S

T

N

D

I

V

G

N

D

-

Y

E

E

I

V

F

Y

N

N

D

F

P

S

E

P

I

Q

S

E

I

L

V

V

I

E

E

E

L

V

-

K

-

P

-

N

-

I

-

L

-

V

-

D

I

V

G
x
S

Y

W

E

D

P

E

A

A

K

H

T

E

F

M

V

Y

L

K

K

V

A

A

I

L

E

G

A

E

G

G

K

I

H

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

L

N

H

Y

G

Y

D

D

E

E

I

L

Y

M

A

N

A

L

A

A

A

K

R

E

K

N

G

N

V

A

E

G

V

I

Q

F

F

F

E
|
E

A

S

A

T

V
|
V

G

M

G
x
A

G

G

I
x
T

P

P

V

I

L

I

S

G

A

V

I

L

K

R

G

E

N

N

L

L

S

L

G

G

N

E

R

N

F

I

S

K

S

R

V

I

F

D

G

A

I

V

M

V

N

N

G

A

T

S

C

T

N

T

Y

F

I

I

L

L

T

T

R

K

M

M

T

S

Q

-

E

E

G

G

A

K

D

T

F

L

E

D

D

D

V

A

L

I

K

E

S

E

A

A

M

K

E

S

L

L

G

G

Y

I

A

L

E

E

P

E

D

D

P

P

T

S

F

K

D

D

I

I

E

D

G

G

V

I

D

D

T

A

A

Y

H

Y

K

K

L

A

A

K

V

I

L

L

A

H

S

W

L

V

C

S

F

Y

G

G

T

E

K

P

I

P

D

E

F

E

N

E

A

-

I

-

H

E

T

R

E

L

G

G

I

I

T

R

A

E

I

V

-

R

S

D

G

A

I

R

D

N

I

V

R

R

F

L

A

V

E

A

Q

Q

F

-

G

-

Y

-

R

-

I

-

K

-

L

-

L

V

A

S

I

K

G

G

K

K

L

I

T

S

D

-

R

-

Q

-

L

-

E

-

V

-

R

-

V

V

H

K

P

P

T

R

M

K

I

L

P

S

L

S

R

D

N

N

P

P

L

L

A

L

D

-

V

V

N

E

G

G

V

V

F

Q

N

N

A

A

I

A

H

V

L

I

T

R

G

T

D

N

F

N

V

L

G

G

P

E

V

V

L

I

F

L

Y

K

G

G

R

P

G

G

A

G

G
|
G

Q

G

N

R

P

V

T

T

A

A

S

S

A

G

I

V

V

F

G

T

D

D

L

I

V

I

F10 (= F11) binding NADPH
T12 (≠ N13) binding NADPH
V13 (≠ I14) binding NADPH
R40 (= R41) binding NADPH; mutation to A: Increases activity with NADP.
K57 (≠ S54) binding NADPH; mutation to A: Increases activity with NADP.
S92 (≠ G81) binding NADPH
S93 (≠ G82) binding NADPH
S114 (≠ A103) binding NADPH
K116 (= K105) binding NADPH
E140 (= E129) binding Na(+)
V143 (= V132) binding Na(+)
A145 (≠ G134) binding Na(+)
T147 (≠ I136) binding Na(+)
G296 (= G303) binding NADPH

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
29% identity, 51% coverage: 3:228/439 of query aligns to 2:236/319 of 3ingA

query
sites
3ingA

E

E

I

I

K

R

V

I

G

I

L

L

I

M

G
|
G

F
x
T

G
|
G

N
|
N

I
x
V

G

G

A

L

G

N

V

V

V

L

R

R

L

I

Q

D

-

A

E

S

N

N

A

R

Q

R

T

R

V

F

Q

S

Q

I

K

K

V

V

G

-

S

V

G

G

I

V

I

S

L
x
D

K
x
S

R

R

I

S

-

Y

-

A

-

S

-

G

A

R

D

N

L

L

D

D

I

I

T

S

S

S

D

-

R

-

G

-

V

I

S

I

V

S

D

N

P
x
K

A

E

M

K

L

T

T

G

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R

D

I

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S

N

D

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R

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E

E

-

D

-

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S

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I

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A

I

A

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D

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L

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L

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V

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D

Y
x
C

E
x
T

P
|
P

A

A

K

S

-

R

-

D

-

G

-

V

-

R

-

E

-

Y

T

S

F

L

V

Y

L

R

K

M

A

A

I

F

E

E

A

S

G

G

K

M

H

N

V

V

T

T

A
|
A

N
|
N

K
|
K

A

S

L

G

L

L

A

A

L

N

H

K

G

W

D

H

E

D

I

I

Y

M

A

D

A

S

A

A

A

N

R

Q

K

N

G

S

V

K

E

Y

V

I

Q

R

F

Y

E

E

A
|
A

A

T

V

V

G

A

G

G

I

V

P

P

V

L

L

F

S

S

A

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I

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K

D

G

Y

N

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L

I

S

L

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P

N

S

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K

F

V

S

K

S

R

V

F

F

R

G

G

I

I

M

V

N

S

G

S

T

T

C

I

N

N

Y

Y

I

V

L

I

T

R

R

N

M

M

T

A

Q

N

E

-

G

G

A

R

D

S

F

L

E

R

D

D

V

V

L

V

K

D

S

D

A

A

M

I

E

K

L

K

G

G

Y

I

A

A

E
|
E

P
x
S

D

N

P

P

T

Q

F

D

D

D

I

L

E

N

G

G

V

L

D
|
D

T

A

A

A

H

R

K
|
K

L

S

A

V

V

I

L

L

A

V

S

N

L

H

C

I

F

F

G

G

T

T

K

E

I

Y

D

T

F

L

N

N

A

D

I

V

H

E

T

Y

E

S

G

G

I

V

active site: D209 (= D201), K213 (= K205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G10), T10 (≠ F11), G11 (= G12), N12 (= N13), V13 (≠ I14), D38 (≠ L39), S39 (≠ K40), K57 (≠ P57), C85 (≠ Y83), T86 (≠ E84), P87 (= P85), A112 (= A103), N113 (= N104), K114 (= K105), A139 (= A130), E198 (= E190), S199 (≠ P191)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 302, 305

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
26% identity, 73% coverage: 1:319/439 of query aligns to 1:320/321 of 3jsaA

query
sites
3jsaA

M

M

N

K

E

T

I

I

K

N

V

L

G

S

L

I

I

F

G
|
G

F
x
L

G
|
G

N
|
N

I
x
V

G

G

A

L

G

N

V

L

R

R

L

I

-

R

-

S

-

F

-

N

Q

E

E

E

N

N

-

R

-

L

-

G

-

L

-

K

-

F

-

N

-

V

-

F

-

V

A

A

Q
x
D

T
x
S

V

L

Q

H

Q

S

-

Y

-

N

-

E

-

R

-

I

K

D

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I

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G

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K

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V

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I

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S

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Y

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K

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E

I

K

A

G

D

S

L

L

D

D

I

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T

L

S

E

D

Y

R

E

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S

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I

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S

V

A

D

S

P

E

A

A

M

L

L

A

T

R

T

-

D

-

V

-

N

-

E

-

I

-

F

-

N

-

D

-

P

-

E

D

I

F

S

D

I

I

V

V

I

V

E

D

L
x
A

I
x
T

G
x
P

G

A

Y

S

E

A

P

D

A

G

K

K

-

K

-

E

-

L

T

A

F

F

V

Y

L

K

K

E

A

T

I

F

E

E

A

N

G

G

K

K

H

D

V

V

T

T

A
|
A

N
|
N

K

K

A

S

L

G

L

L

A

A

L

N

H

F

G

W

D

P

E

E

I

I

Y

M

A

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A

Y

A

A

A

R

R

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K

N

G

N

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E

R

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I

Q

R

F

Y

E

E

A
|
A

A

T

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G

A

G

G

I

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P

P

V

L

L

F

S

S

A

F

I

I

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D

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Y

N

S

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S

L

G

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N

S

R

R

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I

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K

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F

F

R

G

G

I

I

M

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N

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G

L

T

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C

I

N

N

Y

Y

I

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I

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R

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E

M

L

T

A

Q

N

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G

-

A

R

D

E

F

F

E

D

D

D

V

V

L

L

K

S

S

E

A

A

M

T

E

K

L

L

G

G

Y

I

A

V

E

N

P

Y

D

K

P

D

T

-

F

-

D

D

I

L

E

T

G

G

V

L

D
|
D

T

A

A

A

H

R

K
|
K

L

S

A

V

V

I

L

L

A

C

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N

L

H

C

L

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Y

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G

T

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S

I

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D

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S

A

D

I

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H

F

T

Y

E

E

G

G

I

I

T

L

A

D

I

R

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S

-

F

G

G

I

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D

N

I

E

R

R

F

L

A

V

E

T

Q

E

F

T

G

G

-

I

-

V

-

N

-

G

-

K

-

P

-

S

-

A

-

E

Y

S

R

R

I

I

K

K

-

S

-

L

-

D

-

S

-

N

-

D

-

Y

L

L

A

T

I

L

G

G

K

K

L

G

T

S

D

L

D

G

R

Y

Q

Q

L

L

E

Q

V

-

R

-

V

-

H

-

P

-

T

-

M

-

I

-

P

-

L

-

R

-

N

-

P

-

L

-

A

T

D

D

V

T

N

N

G

G

V

T

F

L

N

N

A

V

I

S

H

D

L

L

T

-

G

-

D

-

F

Y

V

D

G

G

P

P

V

Y

L

-

F

-

Y

-

G

-

R

-

G

-

A

-

G

-

Q

-

N

-

P

E

T
|
T

A

A

S

G

A

A

I

V

V

M

G

N

D

D

L

L

V

V

A

I

L

L

S

S

active site: D212 (= D201), K216 (= K205)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), L11 (≠ F11), G12 (= G12), N13 (= N13), V14 (≠ I14), D42 (≠ Q27), S43 (≠ T28), A90 (≠ L79), T91 (≠ I80), P92 (≠ G81), A117 (= A103), N118 (= N104), A144 (= A130), T308 (= T307)

O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 73% coverage: 4:324/439 of query aligns to 558:914/916 of O81852

query
sites
O81852

I

L

K

A

V

M

G

G

L

I

I

V

G

G

F

P

G

G

N

L

I

I

G

G

A

A

G

T

V

L

R

D

L

Q

L

L

Q

R

E

D

N

Q

A

A

Q

A

T

V

V

L

Q

K

Q

Q

K

E

-

F

-

N

-

I

-

D

-

L

-

R

-

V

-

L

-

G

-

I

V

T

G

G

S

S

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M

-

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D

-

I

G

G

I

I

I

D

L

L

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S

R

R

I

W

A

R

D

E

L

L

D

-

I

-

T

L

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N

D

E

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K

G

G

V

T

S

E

V

A

D

D

P

L

A

D

M

K

L

F

T

T

T

Q

D

Q

V

V

N

H

E

G

I

N

F

H

N

F

D

I

P

P

E

N

I

-

S

S

I

V

V

V

I

V

E

D

L

C

I

T

G

A

G

D

Y

S

E

A

P

I

A

A

K

S

T

R

F

Y

V

-

L

Y

K

D

A

W

I

L

E

R

A

K

G

G

K

I

H

H

V

V

V

I

T

T

A

P

N

N

K

K

A

K

L

A

L

N

A

S

L

G

H

P

G

L

D

D

E

Q

I

Y

Y

L

A

K

A

L

A

R

-

D

-

L

A

Q

R

R

K

K

G

S

V

Y

E

T

V

H

Q

Y

F

F

-

Y

E

E

A

A

A

T

V

V

G

G

G

A

G

G

I

L

P

P

V

I

L

I

S

S

A

T

I

L

K

R

G

G

N

L

L

L

-

E

S

T

G

G

N

D

R

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F

I

S

L

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I

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E

G

G

I

I

M

C

N

S

G

G

T

T

C

L

N

S

Y

Y

I

L

L

F

T

N

R

N

M

F

T

V

Q

G

E

D

G

R

A

S

D

-

F

F

E

S

D

E

V

V

L

V

K

T

S

E

A

A

M

K

E

N

L

A

G

G

Y

F

A

T

E

E

P

P

D

D

P

P

T

R

F

D

D

D

I

L

E

S

G

G

V

T

D

D

T

V

A

A

H

R

K

K

L

V

A

I

V

I

L

L

A

A

S

R

L

E

C

S

F

-

G

G

T

L

K

K

I

L

D

D

F

L

-

A

-

D

-

L

-

P

-

I

N

R

A

S

I

L

H

V

T

P

E

E

G

P

I

L

T

K

A

G

I

C

S

T

G

S

I

V

D

E

I

-

R

E

F

F

A

M

E

E

Q

K

F

L

G

P

Y

Q

R

Y

I

D

K

G

L

D

L

L

A

A

I

K

G

E

K

R

L

L

T

D

D

A

D

E

R

N

Q

S

L

G

E

E

V

V

R

L

V

R

H

Y

-

V

-

G

-

V

-

D

-

A

-

V

-

N

-

Q

P

K

T

G

M

T

I

V

P

E

L

L

R

R

-

R

-

Y

-

K

-

E

N

H

P

P

L

F

A

A

D

Q

V

L

N

A

G

G

V

S

F

D

N

N

A

I

I

I

H

A

L

F

T

T

G

T

D

T

F

R

V

Y

G

K

-

D

-

H

P

P

V

L

L

I

F

V

Y

R

G

G

R

P

G

G

A

A

G

G

Q

A

N

Q

P

V

T

T

A

A

S

G

A

G

I

I

V

F

G

S

D

D

L

I

V

L

A

R

L

L

S

A

R

S

S

Y

I

L

A

G

A

A

Sites not aligning to the query:

441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.

O94671 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
33% identity, 26% coverage: 99:213/439 of query aligns to 117:239/376 of O94671

query
sites
O94671

H

N

V

I

V

A

T

T

A

P

N

N

K

K

A

K

L

A

L

F

A

S

L

A

H

S

G

N

D

D

-

V

-

Y

-

Q

E

N

I

I

Y

I

A

K

A

A

A

S

A

K

R

E

K

S

G

G

V

A

E

L

V

L

Q

M

F

H

E

E

A

A

A

S

V

V

G

G

G

A

G

G

I

L

P

P

V

I

L

I

S

S

A

T

I

L

K

K

G

E

N

L

L

I

-

A

S

T

G

G

N

D

R

E

F

I

S

I

S

K

V

I

F

E

G

G

I

I

M

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

V

M

W

T

S

Q

P

E

N

G

G

-

K

-

G

-

T

A

A

D
x
S

F

F

E

S

D

D

V

I

L

V

K

K

S

I

A

A

M

K

E

Q

L

N

G

G

Y

Y

A

T

E

E

P

P

D

D

P

P

T

R

F

D

D

D

I

L

E

N

G

G

V

M

D

D

T

V

A

A

H

R

K

K

L

V

A

T

V

I

L

L

A

S

S

R

L

I

C

A

S201 (≠ D175) modified: Phosphoserine

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
32% identity, 28% coverage: 89:213/439 of query aligns to 100:230/358 of 1tveA

query
sites
1tveA

F

F

V

Y

L

T

K

K

A

F

I

V

E

E

A

N

G

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

L

S

H

D

G

L

D

A

E

T

I

W

Y

K

A

A

A

L

A

F

A

S

R

N

K

K

G

P

V

T

E

N

-

G

-

F

V

V

Q

Y

F

H

E

E

A

A

A

T

V

V

G

G

G

A

G

G

I

L

P

P

V

I

L

I

S

S

A

F

I

L

K

R

G

E

N

I

L

I

-

Q

S

T

G

G

N

D

R

E

F

V

S

E

S

K

V

I

F

E

G

G

I

I

M

F

N

S

G

G

T
|
T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

T

S

Q

T

E

S

G

Q

A

A

D

N

-

D

-

V

-

K

F

F

E

S

D

D

V

V

L

V

K

K

S

V

A

A

M

K

E

K

L

L

G

G

Y

Y

A

T

E

E

P

P

D

D

P

P

T

R

F

D

D
|
D

I

L

E

N

G

G

V

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

V

I

L

V

A

G

S

R

L

I

C

S

active site: D218 (= D201), K222 (= K205)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: K116 (= K105), T175 (= T161), D213 (= D196), D218 (= D201)

Sites not aligning to the query:

binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: 93

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
32% identity, 28% coverage: 89:213/439 of query aligns to 100:230/358 of 1q7gA

query
sites
1q7gA

F

F

V

Y

L

T

K

K

A

F

I

V

E

E

A

N

G

G

K

I

H

S

V

I

V

A

T

T

A
x
P

N

N

K
|
K

A

K

L

A

L

F

A

S

L

S

H

D

G

L

D

A

E

T

I

W

Y

K

A

A

A

L

A

F

A

S

R

N

K

K

G

P

V

T

E

N

-

G

-

F

V

V

Q

Y

F

H

E

E

A
|
A

A

T

V

V

G

G

G

A

G

G

I

L

P

P

V

I

L

I

S

S

A

F

I

L

K

R

G

E

N

I

L

I

-

Q

S

T

G

G

N

D

R

E

F

V

S

E

S

K

V

I

F

E

G

G

I

I

M

F

N
x
S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

T

S

Q

T

E

S

G

Q

A

A

D

N

-

D

-

V

-

K

F

F

E

S

D

D

V

V

L

V

K

K

S

V

A

A

M

K

E

K

L

L

G

G

Y

Y

A

T

E

E

P

P

D

D

P

P

T

R

F

D

D

D

I

L

E

N

G

G

V

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

V

I

L

V

A

G

S

R

L

I

C

S

active site: D218 (= D201), K222 (= K205)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: P114 (≠ A103), K116 (= K105), A143 (= A130), S173 (≠ N159), K222 (= K205)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 13, 14, 15, 39, 91, 92, 93, 97, 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
32% identity, 28% coverage: 89:213/439 of query aligns to 100:230/358 of 1ebuD

query
sites
1ebuD

F

F

V

Y

L

T

K

K

A

F

I

V

E

E

A

N

G

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

L

S

H

D

G

L

D

A

E

T

I

W

Y

K

A

A

A

L

A

F

A

S

R

N

K

K

G

P

V

T

E

N

-

G

-

F

V

V

Q

Y

F

H

E

E

A

A

A

T

V

V

G

G

G

A

G

G

I

L

P

P

V

I

L

I

S

S

A

F

I

L

K

R

G

E

N

I

L

I

-

Q

S

T

G

G

N

D

R

E

F

V

S

E

S

K

V

I

F

E

G

G

I

I

M

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

T

S

Q

T

E

S

G

Q

A

A

D

N

-

D

-

V

-

K

F

F

E

S

D

D

V

V

L

V

K

K

S

V

A

A

M

K

E

K

L

L

G

G

Y

Y

A

T

E

E

P

P

D

D

P

P

T

R

F

D

D

D

I

L

E

N

G

G

V

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

V

I

L

V

A

G

S

R

L

I

C

S

active site: D218 (= D201), K222 (= K205)
binding 3-aminomethyl-pyridinium-adenine-dinucleotide: K116 (= K105)

Sites not aligning to the query:

binding 3-aminomethyl-pyridinium-adenine-dinucleotide: 11, 12, 13, 14, 15, 39, 40, 91, 93, 343

1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
32% identity, 28% coverage: 89:213/439 of query aligns to 100:230/358 of 1ebfA

query
sites
1ebfA

F

F

V

Y

L

T

K

K

A

F

I

V

E

E

A

N

G

G

K

I

H

S

V

I

V

A

T

T

A
x
P

N

N

K

K

A

K

L

A

L

F

A

S

L

S

H

D

G

L

D

A

E

T

I

W

Y

K

A

A

A

L

A

F

A

S

R

N

K

K

G

P

V

T

E

N

-

G

-

F

V

V

Q

Y

F

H

E

E

A

A

A

T

V

V

G

G

G

A

G

G

I

L

P

P

V

I

L

I

S

S

A

F

I

L

K

R

G

E

N

I

L

I

-

Q

S

T

G

G

N

D

R

E

F

V

S

E

S

K

V

I

F

E

G

G

I

I

M

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

T

S

Q

T

E

S

G

Q

A

A

D

N

-

D

-

V

-

K

F

F

E

S

D

D

V

V

L

V

K

K

S

V

A

A

M

K

E

K

L

L

G

G

Y

Y

A

T

E

E

P

P

D

D

P

P

T

R

F

D

D

D

I

L

E

N

G

G

V

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

V

I

L

V

A

G

S

R

L

I

C

S

active site: D218 (= D201), K222 (= K205)
binding nicotinamide-adenine-dinucleotide: P114 (≠ A103)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 12, 13, 14, 15, 39, 40, 92, 93

Query Sequence

>WP_012469284.1 NCBI__GCF_000020385.1:WP_012469284.1
MNEIKVGLIGFGNIGAGVVRLLQENAQTVQQKVGSGIILKRIADLDITSDRGVSVDPAML
TTDVNEIFNDPEISIVIELIGGYEPAKTFVLKAIEAGKHVVTANKALLALHGDEIYAAAA
RKGVEVQFEAAVGGGIPVLSAIKGNLSGNRFSSVFGIMNGTCNYILTRMTQEGADFEDVL
KSAMELGYAEPDPTFDIEGVDTAHKLAVLASLCFGTKIDFNAIHTEGITAISGIDIRFAE
QFGYRIKLLAIGKLTDDRQLEVRVHPTMIPLRNPLADVNGVFNAIHLTGDFVGPVLFYGR
GAGQNPTASAIVGDLVALSRSIAAGVQNRRSAPLGFLDNTITTLPLKPMEEIITSFMLRF
TAQDQPGVLAGIAGVLGKNGISIESMVQTARHNGGQAVPIVIKTHEAREGAIRTALAEID
SFDFISEKSRLIRIEDNLE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory