SitesBLAST

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 61% coverage: 1:377/614 of query aligns to 1:370/557 of P78753

query
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P78753

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Sites not aligning to the query:

391 modified: Phosphoserine
489 modified: Phosphoserine

P22106 Asparagine synthetase B [glutamine-hydrolyzing]; AS-B; EC 6.3.5.4 from Escherichia coli (strain K12) (see 2 papers)
29% identity, 61% coverage: 1:375/614 of query aligns to 1:361/554 of P22106

query
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P22106

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M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H29) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D33) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ Y80) mutation to A: 5-fold decrease in glutamine affinity.
A105 (= A105) mutation to H: Little effect on the kinetic properties.
E349 (≠ Q363) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
27% identity, 57% coverage: 27:375/614 of query aligns to 23:364/509 of 6gq3A

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6gq3A

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active site: L49 (= L50), N74 (= N74), G75 (= G75), T324 (≠ G339), R327 (vs. gap)
binding 5-oxo-l-norleucine: R48 (= R49), V51 (≠ I52), V52 (≠ I53), Y73 (≠ F73), N74 (= N74), G75 (= G75), E76 (= E76), V95 (≠ S98), D96 (= D99)

Sites not aligning to the query:

active site: 4
binding 5-oxo-l-norleucine: 1

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
26% identity, 61% coverage: 1:375/614 of query aligns to 1:377/561 of P08243

query
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P08243

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K

M

Y

C

P

Y

Y

L

L

D

-

R

-

F

-

I

W

I

L

T

C

F

Y

N

N

G

G

E

E

I

I

Y

Y

N

N

Y

H

L

K

E

K

L

M

K

Q

E

Q

E

H

L

F

S

E

N

-

K

-

G

-

Y

F

Y

E

F

Y

I

Q

S

T

T

K

S

V

D

D

T

G

E

E

V

I

I

I

L

L

A

H

A

L

Y

Y

A

D

E

K

W

G

G

G

-

I

E

E

D

Q

C

T

V

I

T

C

H

M

L

L

N

D

G

G

M

V

F

F

A

A

F

F

G

V

L

L

Y

L

D

D

L

T

L

A

E

N

R

K

T

K

L

V

F

F

L

L

A

G

R

R

D

D

R

T

V

Y

G

G

E

V

K

R

P

P

L

L

Y

F

Y

K

S

A

K

M

N

T

E

E

N

D

E

G

F

F

F

L

-

A

F

V

F

C

S

S

E

E

P

A

K

K

Q

G

V

L

I

V

S

T

S

-

G

-

I

-

E

-

G

-

I

-

P

-

N

-

E

-

V

-

A

-

I

-

R

-

Q

-

Y

-

L

-

E

-

Y

-

Q

-

F

-

T

L

L

K

S

H

A

S

Q

A

T

T

F

P

F

F

T

L

G

K

I

V

Y

E

K

P

L

F

L

L

P

P

G

G

H

H

C

Y

A

E

T

V

L

L

-

D

-

L

-

K

R

P

N

N

G

G

V

K

F

V

K

A

S

S

R

V

E

E

-

M

-

V

-

K

Y

Y

W

H

S

H

L

C

A

R

D

D

V
|
V

E

P

V

L

-

H

-

A

-

L

-

Y

-

D

-

N

-

V

-

E

-

K

-

L

-

F

-

P

D

G

Y

F

S

E

I

I

S

E

Y

T

A

V

E

K

A

N

K

N

L

L

R

R

I

I

K

-

N

-

L

L

V

F

E

N

D

N

S

A

L

V

K

K

I

K

R

R

L

L

R

M

S

T

D

D

V

R

P

R

V

I

A

G

S

C

Y

L

L

L

S

S

G

G

I

L

D

D

S

S

T

S

I

L

I

V

A

A

T

A

S

T

A

L

K

K

E

Q

L

L

S

K

-

E

-

A

-

Q

-

V

-

Q

-

Y

S

P

L

L

S

Q

T

T

Y

F

T

A

F

I

T

G

S

M

K

E

K

D

Y

S

P

P

R

-

F

-

D

-

E

D

S

L

Q

L

N

A

A

A

R

R

L

K

T

V

A

A

D

D

L

H

I

I

H

G

A

S

D

E

H

H

H

Y

E

E

I

V

E

L

I

F

E

N

N

S

A

E

D

E

T

G

L

I

S

Q

L

A

W

L

Q

D

N

E

S

V

T

I

Y

F

F

S

M

L

D

E

E

T

P

Y

E

D

V

I

G

T

Y

T

-

V

-

R

S

A

L

S

L

V

S

G

Q

M

M

Y

A

L

V

I

S

S

R

K

E

Y

V

I

A

R

K

K

Q

N

T

T

K

D

-

S

-

V

V

V

V

I

L
x
F

G

S

G

G

Q

E

G

G

G

S

D

D

E

E

L

L

F

T

F

Q

G

G

Y

Y

A

I

W

Y

Y

F

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (= V225) to E: in dbSNP:rs1049674
F362 (≠ L360) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

1jgtB Crystal structure of beta-lactam synthetase (see paper)
25% identity, 56% coverage: 60:405/614 of query aligns to 49:387/500 of 1jgtB

query
sites
1jgtB

H

H

Q

A

P

P

M

S

C

V

Y

A

L

P

D

D

R

R

F

A

I

V

I

A

-

R

-

S

-

L

-

T

-

G

-

A

-

P

-

T

-

A

T

V

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

Y

R

L

D

E

E

L

L

K

-

E

-

L

L

S

S

N

V

K

L

G

P

Y

A

Y

G

F

P

I

A

S

P

T

E

S

G

D

D

T

A

E

E

V

L

I

V

L

L

A

R

A

L

Y

L

A

E

E

R

W

Y

G

D

E

L

D

H

C

A

V

F

T

R

H

L

L

V

N

N

G

G

M

R

F

F

A

A

F

T

G

-

L

V

Y

V

D

R

L

T

L

G

E

D

R

R

T

V

L

L

F

-

L

L

A

A

R

T

D

D

R

H

V

A

G

G

E

S

K

V

P

P

L

L

Y

Y

Y

T

S

C

K

V

N

A

E

P

N

G

E

E

F

V

F

R

F

A

F

S

S

T

E

E

P

A

K

K

Q

A

V

L

I

A

S

A

S

H

G

R

I

D

I

P

E

K

G

G

I

F

P

P

N

L

E

A

V

D

A

A

I

R

R

R

Q

V

Y

-

L

-

E

-

Y

-

Q

-

F

-

T

-

L

-

S

-

A

-

Q

-

T

A

F

G

F

L

T

T

G

G

I

V

Y

Y

K

Q

L

V

L

P

P

A

G

G

H

A

C

V

A

M

T

D

-

I

-

D

L

L

R

G

N

S

G

G

V

T

F

A

K

V

S

T

R

H

E

R

Y

T

W

W

S

T

L

P

A

G

D

L

V

S

E

R

V

-

D

-

Y

R

S

I

I

L

S

P

Y

E

A

G

E

E

A

A

K

V

L

A

R

A

I

V

K

R

N

A

L

A

V

L

E

E

D

K

S

A

L

V

K

A

I

Q

R

R

L

V

R

T

-

P

S

G

D

D

V

T

P

P

V

L

A

V

S

-

Y
x
V

L
|
L

S
|
S

G

G

G
|
G

I
|
I

D
|
D

S
|
S

T

S

I

G

I

V

A

A

T

A

S

C

-

A

-

H

-

R

A

A

K

G

E

E

L

L

S

D

S

T

L

V

S
|
S

T
x
M

Y

G

T

T

F

D

T

T

S

S

K

N

K

E

Y

F

P

-

R

-

F

-

D

-

E

-

S

-

Q

R

N

E

A

A

R

R

L

A

T

V

A

V

D

D

L

H

I

L

H

R

A

T

D

R

H

H

H

R

E

E

I

I

E

T

I

I

E

P

N

T

A

T

D

E

T

L

L

L

-

A

-

Q

-

L

-

P

-

Y

S

A

L

V

W

W

Q

A

N

S

S

E

T

S

Y

V

F

-

M

-

D

-

E

D

P

P

E
x
D

V

I

G

I

Y

E

S
x
Y

L

L

S

P

Q
x
L

M

T

A

A

V

L

S

Y

R

R

E

A

V

L

A

D

K

G

Q

P

T

E

K

R

V

R

V

I

L

L

G

T

G
|
G

Q
x
Y

G
|
G

G

A

D
|
D

E
x
I

L

P

F

L

F

G

G

G

Y
x
M

A

H

W

R

Y

E

S

D

N

R

L

L

L

P

L

A

K

L

S

D

F

T

L

V

S

L

F

A

S

H

S
x
D

V

M

A

A

D

T

F

F

K

D

L

G

L

L

D

N

K
x
E

V

M

K

S

V

P

V

V

I

L

S

S

F

T

L

L

active site: A73 (≠ N74), G74 (= G75), D319 (≠ E337), Y345 (≠ Q363), E379 (≠ K397)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ Y261), L245 (= L262), S246 (= S263), G248 (= G265), I249 (= I266), D250 (= D267), S251 (= S268), S269 (= S283), M270 (≠ T284), L327 (≠ Q345), G344 (= G362), Y345 (≠ Q363), D348 (= D366)
binding n2-(carboxyethyl)-l-arginine: Y323 (≠ S341), Y345 (≠ Q363), G346 (= G364), D348 (= D366), I349 (≠ E367), M354 (≠ Y372), D370 (≠ S388), E379 (≠ K397)
binding magnesium ion: D250 (= D267), D348 (= D366)

Sites not aligning to the query:

active site: 440
binding diphosphomethylphosphonic acid adenosyl ester: 420, 440

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
25% identity, 56% coverage: 60:405/614 of query aligns to 45:379/496 of 1mbzA

query
sites
1mbzA

H

H

Q

A

P

P

M

S

C

V

Y

A

L

P

D

D

R

R

F

A

I

V

I

A

-

R

-

S

-

L

-

T

-

G

-

A

-

P

-

T

-

A

T

V

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

Y

R

L

D

E

E

L

L

K

-

E

-

L

L

S

S

N

V

K

L

G

P

Y

A

Y

G

F

P

I

A

S

P

T

E

S

G

D

D

T

A

E

E

V

L

I

V

L

L

A

R

A

L

Y

L

A

E

E

R

W

Y

G

D

E

L

D

H

C

A

V

F

T

R

H

L

L

V

N

N

G

G

M

R

F

F

A

A

F

T

G

-

L

V

Y

V

D

R

L

T

L

G

E

D

R

R

T

V

L

L

F

-

L

L

A

A

R

T

D

D

R

H

V

A

G

G

E

S

K

V

P

P

L

L

Y

Y

Y

T

S

C

K

V

N

A

E

P

N

G

E

E

F

V

F

R

F

A

F

S

S

T

E

E

P

A

K

K

Q

A

V

L

I

A

S

A

S

H

G

G

I

F

I

P

E

L

G

A

I

D

P

A

N

R

E

R

V

V

A

A

I

-

R

-

Q

-

Y

-

L

-

E

-

Y

-

Q

-

F

-

T

-

L

-

S

-

A

-

Q

-

T

-

F

G

F

L

T

T

G

G

I

V

Y

Y

K

Q

L

V

L

P

P

A

G

G

H

A

C

V

A

M

T

D

-

I

-

D

L

L

R

G

N

S

G

G

V

T

F

A

K

V

S

T

R

H

E

R

Y

T

W

W

S

T

L

P

A

G

D

L

V

S

E

R

V

-

D

-

Y

R

S

I

I

L

S

P

Y

E

A

G

E

E

A

A

K

V

L

A

R

A

I

V

K

R

N

A

L

A

V

L

E

E

D

K

S

A

L

V

K

A

I

Q

R

R

L

V

R

T

-

P

S

G

D

D

V

T

P

P

V

L

A

V

S

-

Y
x
V

L
|
L

S
|
S

G

G

G
|
G

I

I

D
|
D

S
|
S

T

S

I

G

I

V

A

A

T

A

S

C

-

A

-

H

-

R

A

A

K

G

E

E

L

L

S

D

S

T

L

V

S
|
S

T
x
M

Y

G

T

T

F

D

T

T

S

S

K

N

K

E

Y

F

P

-

R

-

F

-

D

-

E

-

S

-

Q

R

N

E

A

A

R

R

L

A

T

V

A

V

D

D

L

H

I

L

H

R

A

T

D

R

H

H

H

R

E

E

I

I

E

T

I

I

E

P

N

T

A

T

D

E

T

L

L

L

-

A

-

Q

-

L

-

P

-

Y

S

A

L

V

W

W

Q

A

N

S

S

E

T

S

Y

V

F

-

M

-

D

-

E

D

P

P

E
x
D

V

I

G

I

Y

E

S
x
Y

L

L

S

P

Q
x
L

M

T

A

A

V

L

S

Y

R

R

E

A

V

L

A

D

K

G

Q

P

T

E

K

R

V

R

V

I

L

L

G

T

G
|
G

Q
x
Y

G
|
G

G

A

D
|
D

E
x
I

L

P

F

L

F

G

G

G

Y

M

A

H

W

R

Y

E

S

D

N

R

L

L

L

P

L

A

K

L

S

D

F

T

L

V

S

L

F

A

S

H

S
x
D

V

M

A

A

D

T

F

F

K

D

L

G

L

L

D

N

K
x
E

V

M

K

S

V

P

V

V

I

L

S

S

F

T

L

L

active site: A69 (≠ N74), G70 (= G75), D311 (≠ E337), Y337 (≠ Q363), E371 (≠ K397)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ Y261), L237 (= L262), S238 (= S263), S243 (= S268), S261 (= S283), M262 (≠ T284), Y315 (≠ S341), L319 (≠ Q345), G336 (= G362), Y337 (≠ Q363), G338 (= G364), D340 (= D366), I341 (≠ E367), D362 (≠ S388), E371 (≠ K397)
binding magnesium ion: D242 (= D267), D340 (= D366)
binding pyrophosphate 2-: S238 (= S263), G240 (= G265), D242 (= D267), S243 (= S268), D340 (= D366)

Sites not aligning to the query:

active site: 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 432, 434, 435
binding pyrophosphate 2-: 412, 432, 433

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
25% identity, 56% coverage: 60:405/614 of query aligns to 41:374/491 of 1mc1A

query
sites
1mc1A

H

H

Q

A

P

P

M

S

C

V

Y

A

L

P

D

D

R

R

F

A

I

V

I

A

-

R

-

S

-

L

-

T

-

G

-

A

-

P

-

T

-

A

T

V

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

Y

R

L

D

E

E

L

L

K

-

E

-

L

L

S

S

N

V

K

L

G

P

Y

A

Y

G

F

P

I

A

S

P

T

E

S

G

D

D

T

A

E

E

V

L

I

V

L

L

A

R

A

L

Y

L

A

E

E

R

W

Y

G

D

E

L

D

H

C

A

V

F

T

R

H

L

L

V

N

N

G

G

M

R

F

F

A

A

F

T

G

-

L

V

Y

V

D

R

L

T

L

G

E

D

R

R

T

V

L

L

F

-

L

L

A

A

R

T

D

D

R

H

V

A

G

G

E

S

K

V

P

P

L

L

Y

Y

Y

T

S

C

K

V

N

A

E

P

N

G

E

E

F

V

F

R

F

A

F

S

S

T

E

E

P

A

K

K

Q

A

V

L

I

A

S

A

S

-

G

-

I

-

E

-

G

G

I

F

P

P

N

L

E

A

V

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active site: A65 (≠ N74), G66 (= G75), D306 (≠ E337), Y332 (≠ Q363), E366 (≠ K397)
binding adenosine monophosphate: V231 (≠ Y261), S233 (= S263), S238 (= S268), S256 (= S283), M257 (≠ T284), G331 (= G362)
binding magnesium ion: D237 (= D267), D335 (= D366)
binding deoxyguanidinoproclavaminic acid: Y310 (≠ S341), Y332 (≠ Q363), G333 (= G364), I336 (≠ E367), D357 (≠ S388), E366 (≠ K397)
binding pyrophosphate 2-: S233 (= S263), G235 (= G265), D237 (= D267), S238 (= S268), D335 (= D366)

Sites not aligning to the query:

active site: 427
binding adenosine monophosphate: 427, 430
binding deoxyguanidinoproclavaminic acid: 427
binding pyrophosphate 2-: 407, 427, 428

Query Sequence

>WP_012469560.1 NCBI__GCF_000020385.1:WP_012469560.1
MCGIAGIVYRNKKSNHSEQIAEMLKLIHHRGPDGMGVFEAENVVLGHRRLAIIDLSPSGH
QPMCYLDRFIITFNGEIYNYLELKEELSNKGYYFISTSDTEVILAAYAEWGEDCVTHLNG
MFAFGLYDLLERTLFLARDRVGEKPLYYSKNENEFFFFSEPKQVISSGIIEGIPNEVAIR
QYLEYQFTLSAQTFFTGIYKLLPGHCATLRNGVFKSREYWSLADVEVDYSISYAEAKLRI
KNLVEDSLKIRLRSDVPVASYLSGGIDSTIIATSAAKELSSLSTYTFTSKKYPRFDESQN
ARLTADLIHADHHEIEIENADTLSLWQNSTYFMDEPEVGYSLLSQMAVSREVAKQTKVVL
GGQGGDELFFGYAWYSNLLLKSFLSFSSVADFKLLDKVKVVISFLLNSPKRTALRLIFDS
FKSFGRPLSEIYIDTWRGYGCFSLLQDIKIKEGANSPVSGCLKLSELKKFEFNYWLHGLL
HVEDRSSMAHSLESRVPLLDHRLVEFVFSLPPHFMIDGVLNKKIFIDAFSDILPNHVKNN
KQKMGYVSPIHSWLSDQHVTAFIKDVISNKSSFIYHFVDYYKMKSLQINDRQLWMLISLE
IWHNIFIKRDIKSC

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory