SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_012469705.1 NCBI__GCF_000020385.1:WP_012469705.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

5j6fA Crystal structure of dah7ps-cm complex from geobacillus sp. With prephenate (see paper)
32% identity, 85% coverage: 3:83/95 of query aligns to 4:84/352 of 5j6fA

query
sites
5j6fA
I
 
L
N
 
D
E
 
E
T
 
L
R
 
R
M
 
A
R
 
R
I
 
V
D
 
D
L
 
E
L
 
I
D
 
N
D
 
L
V
 
Q
L
 
L
L
 
L
R
 
K
I
 
L
F
 
I
N
 
N
E
 
E
R
|
R
A
 
G
R
 
R
L
 
L
A
 
V
L
 
Q
E
 
E
I
|
I
G
 
G
H
 
K
Y
 
I
K
|
K
K
 
E
L
 
A
L
 
Q
D
 
G
L
 
T
P
 
H
V
 
R
Y
|
Y
D
|
D
P
 
P
A
 
V
R
|
R
E
 
E
K
 
R
K
 
K
I
x
M
F
 
L
A
 
D
R
 
L
M
 
I
K
 
S
S
 
E
D
 
H
N
 
N
P
 
D
G
 
G
P
 
P
L
 
F
D
 
E
D
 
T
G
 
S
A
 
T
I
 
L
V
 
Q
R
 
H
L
 
I
F
 
F
E
 
K
R
 
E
V
 
I
I
 
F
D
 
K
E
 
A
S
 
A
R
 
L
R
 
E
L
 
L
E
 
Q

Sites not aligning to the query:

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
36% identity, 87% coverage: 7:89/95 of query aligns to 11:94/386 of P0A9J8

query
sites
P0A9J8
R
|
R
M
 
E
R
 
K
I
 
I
D
 
S
L
 
A
L
 
L
D
 
D
D
 
E
V
 
K
L
 
L
L
 
L
R
 
A
I
 
L
F
 
L
N
 
A
E
 
E
R
|
R
A
 
R
R
 
E
L
 
L
A
 
A
L
 
V
E
 
E
I
 
V
G
 
G
H
 
K
Y
 
A
K
|
K
K
 
L
L
 
L
L
 
S
D
 
H
L
 
R
P
 
P
V
 
V
Y
 
R
D
 
D
P
 
I
A
 
D
R
 
R
E
|
E
K
 
R
K
 
D
I
 
L
F
 
L
A
 
E
R
 
R
M
 
L
K
 
I
S
 
T
-
 
L
D
 
G
N
 
K
P
 
A
G
 
H
P
 
H
L
 
L
D
 
D
D
 
A
G
 
H
A
 
Y
I
 
I
V
 
T
R
 
R
L
 
L
F
 
F
E
 
Q
R
 
L
V
 
I
I
 
I
D
 
E
E
 
D
S
 
S
R
 
V
R
 
L
L
 
T
E
x
Q
R
 
Q
I
 
A
M
 
L
T
 
L
Q
 
Q
Q
 
Q

5gmuB Crystal structure of chorismate mutase like domain of bifunctional dahp synthase of bacillus subtilis in complex with chlorogenic acid (see paper)
29% identity, 74% coverage: 7:76/95 of query aligns to 9:78/87 of 5gmuB

query
sites
5gmuB
R
 
R
M
 
Q
R
 
K
I
 
A
D
 
D
L
 
E
L
 
L
D
 
N
D
 
L
V
 
Q
L
 
I
L
 
L
R
 
K
I
 
L
F
 
I
N
 
N
E
 
E
R
|
R
A
 
G
R
 
N
L
 
V
A
 
V
L
 
K
E
 
E
I
 
I
G
 
G
H
 
K
Y
 
A
K
 
K
K
 
E
L
 
A
L
 
Q
D
 
G
L
 
V
P
 
N
V
x
R
Y
x
F
D
|
D
P
 
P
A
 
V
R
 
R
E
|
E
K
 
R
K
 
T
I
 
M
F
 
L
A
 
N
R
 
N
M
 
I
K
 
I
S
 
E
D
 
N
N
 
N
P
 
D
G
 
G
P
 
P
L
 
F
D
 
E
D
 
N
G
 
S
A
 
T
I
 
I
V
 
Q
R
 
H
L
 
I
F
 
F
E
 
K
R
 
E
V
 
I
I
x
F

Sites not aligning to the query:

1ecmB Atomic structure of the buried catalytic pocket of escherichia coli chorismate mutase
35% identity, 88% coverage: 7:90/95 of query aligns to 6:90/95 of 1ecmB

query
sites
1ecmB
R
 
R
M
 
E
R
 
K
I
 
I
D
 
S
L
 
A
L
 
L
D
 
D
D
 
E
V
 
K
L
 
L
L
 
L
R
 
A
I
 
L
F
 
L
N
 
A
E
 
E
R
|
R
A
 
R
R
 
E
L
 
L
A
 
A
L
 
V
E
 
E
I
x
V
G
 
G
H
 
K
Y
 
A
K
|
K
K
 
L
L
 
L
L
 
S
D
 
H
L
 
R
P
 
P
V
 
V
Y
x
R
D
|
D
P
 
I
A
 
D
R
|
R
E
|
E
K
 
R
K
 
D
I
 
L
F
 
L
A
 
E
R
 
R
M
 
L
K
 
I
S
 
T
-
 
L
D
 
G
N
 
K
P
 
A
G
 
H
P
 
H
L
 
L
D
 
D
D
 
A
G
 
H
A
 
Y
I
 
I
V
 
T
R
 
R
L
 
L
F
 
F
E
 
Q
R
 
L
V
 
I
I
|
I
D
 
E
E
 
D
S
|
S
R
 
V
R
 
L
L
 
T
E
x
Q
R
 
Q
I
 
A
M
 
L
T
 
L
Q
 
Q
Q
 
Q
E
 
H

P39912 Protein AroA(G); EC 2.5.1.54; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see paper)
27% identity, 81% coverage: 7:83/95 of query aligns to 10:86/358 of P39912

query
sites
P39912
R
 
R
M
 
Q
R
 
K
I
 
A
D
 
D
L
 
E
L
 
L
D
 
N
D
 
L
V
 
Q
L
 
I
L
 
L
R
 
K
I
 
L
F
 
I
N
 
N
E
 
E
R
 
R
A
 
G
R
 
N
L
 
V
A
 
V
L
 
K
E
 
E
I
 
I
G
 
G
H
 
K
Y
 
A
K
 
K
K
 
E
L
 
A
L
 
Q
D
 
G
L
 
V
P
 
N
V
 
R
Y
 
F
D
 
D
P
 
P
A
 
V
R
 
R
E
 
E
K
 
R
K
 
T
I
 
M
F
 
L
A
 
N
R
 
N
M
 
I
K
 
I
S
 
E
D
 
N
N
 
N
P
 
D
G
 
G
P
 
P
L
 
F
D
 
E
D
 
N
G
 
S
A
 
T
I
 
I
V
 
Q
R
 
H
L
 
I
F
 
F
E
 
K
R
 
E
V
 
I
I
 
F
D
 
K
E
 
A
S
 
G
R
 
L
R
 
E
L
 
L
E
 
Q

Sites not aligning to the query:

3nvtA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e (see paper)
30% identity, 83% coverage: 5:83/95 of query aligns to 1:74/345 of 3nvtA

query
sites
3nvtA
E
 
E
T
 
L
R
 
R
M
 
T
R
 
Q
I
 
V
D
 
D
L
 
Q
L
 
L
D
 
N
D
 
I
V
 
D
L
 
L
L
 
L
R
 
E
I
 
L
F
 
I
N
 
S
E
 
K
R
 
R
A
 
A
R
 
N
L
 
L
A
 
V
L
 
Q
E
 
E
I
 
I
G
 
G
H
 
K
Y
 
I
K
 
K
K
 
G
L
 
R
L
 
-
D
 
-
L
 
-
P
 
-
V
 
-
Y
 
F
D
 
D
P
 
P
A
 
L
R
 
R
E
 
E
K
 
R
K
 
E
I
 
M
F
 
L
A
 
N
R
 
T
M
 
I
K
 
L
S
 
A
D
 
A
N
 
N
P
 
E
G
 
G
P
 
P
L
 
F
D
 
E
D
 
D
G
 
S
A
 
T
I
 
V
V
 
Q
R
 
K
L
 
L
F
 
F
E
 
K
R
 
E
V
 
I
I
 
F
D
 
K
E
 
A
S
 
G
R
 
L
R
 
E
L
 
L
E
 
Q

Sites not aligning to the query:

2gtvX Nmr structure of monomeric chorismate mutase from methanococcus jannaschii (see paper)
29% identity, 84% coverage: 5:84/95 of query aligns to 7:95/104 of 2gtvX

query
sites
2gtvX
E
 
E
T
 
I
R
|
R
M
 
K
R
 
K
I
 
I
D
 
D
L
 
E
L
 
I
D
 
D
D
 
N
V
 
K
L
 
I
L
 
L
R
 
K
-
 
A
-
 
R
-
 
W
-
 
P
-
 
W
-
 
A
-
 
E
-
 
K
I
 
L
F
 
I
N
 
A
E
 
E
R
|
R
A
 
N
R
 
S
L
 
L
A
 
A
L
 
K
E
 
D
I
x
V
G
 
A
H
 
E
Y
 
I
K
|
K
K
 
N
L
 
Q
L
 
L
D
 
G
L
 
I
P
 
P
V
 
I
Y
 
N
D
|
D
P
 
P
A
 
E
R
|
R
E
|
E
K
 
K
K
 
Y
I
 
I
F
 
Y
A
 
D
R
 
R
M
 
I
-
 
R
K
 
K
S
 
L
D
 
C
N
 
K
P
 
E
G
 
H
P
 
N
L
 
V
D
 
D
D
 
E
G
 
N
A
 
I
I
 
G
V
 
I
R
 
K
L
 
I
F
 
F
E
 
Q
R
 
R
V
 
L
I
 
I
D
 
E
E
 
H
S
x
N
R
 
K
R
 
A
L
 
L
E
x
Q
R
 
K

3tfcA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e in complex with phosphoenolpyruvate (see paper)
30% identity, 81% coverage: 7:83/95 of query aligns to 2:73/343 of 3tfcA

query
sites
3tfcA
R
 
R
M
 
T
R
 
Q
I
 
V
D
 
D
L
 
Q
L
 
L
D
 
N
D
 
I
V
 
D
L
 
L
L
 
L
R
 
E
I
 
L
F
 
I
N
 
S
E
 
K
R
 
R
A
 
A
R
 
N
L
 
L
A
 
V
L
 
Q
E
 
E
I
 
I
G
 
G
H
 
K
Y
 
I
K
 
K
K
 
L
L
 
R
L
 
-
D
 
-
L
 
-
P
 
-
V
 
-
Y
 
F
D
 
D
P
 
P
A
 
L
R
 
R
E
 
E
K
 
R
K
 
E
I
 
M
F
 
L
A
 
N
R
 
T
M
 
I
K
 
L
S
 
A
D
 
A
N
 
N
P
 
E
G
 
G
P
 
P
L
 
F
D
 
E
D
 
D
G
 
S
A
 
T
I
 
V
V
 
Q
R
 
K
L
 
L
F
 
F
E
 
K
R
 
E
V
 
I
I
 
F
D
 
K
E
 
A
S
 
G
R
 
L
R
 
E
L
 
L
E
 
Q

Sites not aligning to the query:

Query Sequence

>WP_012469705.1 NCBI__GCF_000020385.1:WP_012469705.1
MNINETRMRIDLLDDVLLRIFNERARLALEIGHYKKLLDLPVYDPAREKKIFARMKSDNP
GPLDDGAIVRLFERVIDESRRLERIMTQQEGQEEC

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory