SitesBLAST

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
36% identity, 87% coverage: 7:89/95 of query aligns to 11:94/386 of P0A9J8

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P0A9J8

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R11 (= R7) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R24) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (= K35) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E48) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
Q88 (≠ E83) mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

5gmuB Crystal structure of chorismate mutase like domain of bifunctional dahp synthase of bacillus subtilis in complex with chlorogenic acid (see paper)
29% identity, 74% coverage: 7:76/95 of query aligns to 9:78/87 of 5gmuB

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5gmuB

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binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: R26 (= R24), R44 (≠ V42), F45 (≠ Y43), D46 (= D44), E50 (= E48), F78 (≠ I76)

Sites not aligning to the query:

binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: 79, 82, 85

1ecmB Atomic structure of the buried catalytic pocket of escherichia coli chorismate mutase
35% identity, 88% coverage: 7:90/95 of query aligns to 6:90/95 of 1ecmB

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1ecmB

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binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R23 (= R24), V30 (≠ I31), K34 (= K35), R42 (≠ Y43), D43 (= D44), R46 (= R47), E47 (= E48), I76 (= I76), S79 (= S79), Q83 (≠ E83)

P39912 Protein AroA(G); EC 2.5.1.54; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see paper)
27% identity, 81% coverage: 7:83/95 of query aligns to 10:86/358 of P39912

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P39912

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2 modified: Phosphoserine

3nvtA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e (see paper)
30% identity, 83% coverage: 5:83/95 of query aligns to 1:74/345 of 3nvtA

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3nvtA

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binding manganese (ii) ion: 114, 284, 310, 321

2gtvX Nmr structure of monomeric chorismate mutase from methanococcus jannaschii (see paper)
29% identity, 84% coverage: 5:84/95 of query aligns to 7:95/104 of 2gtvX

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2gtvX

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binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R9 (= R7), R34 (= R24), V41 (≠ I31), K45 (= K35), D54 (= D44), R57 (= R47), E58 (= E48), N90 (≠ S79), Q94 (≠ E83)

3tfcA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e in complex with phosphoenolpyruvate (see paper)
30% identity, 81% coverage: 7:83/95 of query aligns to 2:73/343 of 3tfcA

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3tfcA

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binding manganese (ii) ion: 113, 283, 309, 320
binding phosphoenolpyruvate: 137, 193, 196, 218, 248, 283

Query Sequence

>WP_012469705.1 NCBI__GCF_000020385.1:WP_012469705.1
MNINETRMRIDLLDDVLLRIFNERARLALEIGHYKKLLDLPVYDPAREKKIFARMKSDNP
GPLDDGAIVRLFERVIDESRRLERIMTQQEGQEEC

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory