SitesBLAST
Comparing WP_012469913.1 NCBI__GCF_000020385.1:WP_012469913.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2y27B Crystal structure of paak1 in complex with atp from burkholderia cenocepacia (see paper)
52% identity, 98% coverage: 7:433/435 of query aligns to 5:427/427 of 2y27B
- binding adenosine-5'-triphosphate: K65 (= K67), S90 (= S92), S91 (= S93), G92 (= G94), T93 (= T95), T94 (= T96), F138 (= F140), A211 (= A213), E212 (= E214), P213 (= P215), D232 (= D234), I233 (= I235), Y234 (= Y236), G235 (= G237), L236 (= L238), S237 (= S239), D302 (= D304), I320 (≠ M325), R323 (= R328), K419 (= K425)
- binding magnesium ion: V200 (≠ K202), S202 (= S204), L204 (= L206), M226 (≠ L228), G227 (= G229), Q347 (≠ I352), L350 (≠ V355)
2y4oA Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
52% identity, 98% coverage: 7:434/435 of query aligns to 7:433/433 of 2y4oA
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (≠ Y135), F140 (= F140), A213 (= A213), E214 (= E214), P215 (= P215), I235 (= I235), G237 (= G237), L238 (= L238), S239 (= S239), P244 (= P244), D304 (= D304), R325 (= R328), I331 (= I334), N336 (= N339)
2y4oB Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
52% identity, 98% coverage: 7:433/435 of query aligns to 7:432/432 of 2y4oB
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (≠ Y135), F140 (= F140), G212 (= G212), A213 (= A213), E214 (= E214), P215 (= P215), I235 (= I235), G237 (= G237), L238 (= L238), S239 (= S239), P244 (= P244), D304 (= D304), R325 (= R328), I331 (= I334), N336 (= N339)
- binding magnesium ion: S204 (= S204), V228 (≠ L228)
2y4nA Paak1 in complex with phenylacetyl adenylate (see paper)
52% identity, 98% coverage: 7:434/435 of query aligns to 5:426/426 of 2y4nA
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: Y131 (= Y135), F136 (= F140), G138 (= G142), G208 (= G212), A209 (= A213), E210 (= E214), P211 (= P215), I231 (= I235), Y232 (= Y236), G233 (= G237), L234 (= L238), S235 (= S239), P240 (= P244), D300 (= D304), R321 (= R328), K417 (= K425)
- binding magnesium ion: V198 (≠ K202), S200 (= S204), Q345 (≠ I352), L348 (≠ V355)
4r1mA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.48 a resolution
47% identity, 99% coverage: 2:433/435 of query aligns to 4:433/435 of 4r1mA
- binding adenosine monophosphate: A215 (= A213), E216 (= E214), P217 (= P215), N236 (≠ D234), S237 (≠ I235), F238 (≠ Y236), G239 (= G237), M240 (≠ L238), T241 (≠ S239), D305 (= D304), R329 (= R328), I335 (= I334), N340 (= N339)
- binding zinc ion: C252 (= C250), H259 (= H258), C314 (= C313), C316 (= C315)
4r1lA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.42 a resolution
46% identity, 99% coverage: 2:432/435 of query aligns to 4:433/433 of 4r1lA
- binding adenosine-5'-diphosphate: A215 (= A213), E216 (= E214), P217 (= P215), S237 (≠ I235), F238 (≠ Y236), G239 (= G237), M240 (≠ L238), T241 (≠ S239), D305 (= D304), R329 (= R328), N340 (= N339)
- binding adenosine monophosphate: A215 (= A213), E216 (= E214), P217 (= P215), S237 (≠ I235), F238 (≠ Y236), G239 (= G237), M240 (≠ L238), T241 (≠ S239), D305 (= D304), R329 (= R328), N340 (= N339)
- binding coenzyme a: S136 (≠ A134), A164 (≠ G162), G165 (= G163), N166 (= N164), S167 (≠ T165), I185 (≠ T183), Y188 (= Y186), K337 (≠ R336), T408 (= T408)
- binding zinc ion: C252 (= C250), H259 (= H258), C314 (= C313), C316 (= C315)
6he0A Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in complex with 2-hib-amp and coa in the thioesterfication state (see paper)
30% identity, 100% coverage: 2:435/435 of query aligns to 22:466/477 of 6he0A
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] 2-methyl-2-oxidanyl-propanoate: S241 (≠ G212), G242 (≠ A213), E243 (= E214), P244 (= P215), G267 (≠ Y236), S268 (≠ G237), M269 (≠ L238), A270 (≠ S239), D335 (= D304), I357 (≠ M325), N371 (= N339)
- binding adenosine monophosphate: G242 (≠ A213), E243 (= E214), P244 (= P215), C266 (≠ I235), G267 (≠ Y236), S268 (≠ G237), A270 (≠ S239), E271 (= E240), D335 (= D304), N371 (= N339)
- binding coenzyme a: Y166 (≠ F140), A188 (≠ G162), G189 (= G163), P191 (vs. gap), S194 (≠ T165), Y210 (≠ T181), G211 (≠ C182), T212 (= T183), Y215 (= Y186), H218 (≠ Y189), R368 (= R336), G369 (= G337), M401 (≠ L369), V439 (= V407), R440 (≠ T408)
6hdyA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with s3-hb-amp (see paper)
29% identity, 100% coverage: 2:435/435 of query aligns to 22:463/474 of 6hdyA
- binding (3s)-3-hydroxybutanoic acid: Y162 (≠ F140), S237 (≠ G212), G263 (≠ Y236), S264 (≠ G237), M265 (≠ L238), A266 (≠ S239), F271 (≠ G245)
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (3~{S})-3-oxidanylbutanoate: Y162 (≠ F140), G164 (= G142), S237 (≠ G212), G238 (≠ A213), E239 (= E214), P240 (= P215), C262 (≠ I235), G263 (≠ Y236), S264 (≠ G237), A266 (≠ S239), F271 (≠ G245), D331 (= D304), I353 (≠ M325), R356 (= R328), K453 (= K425)
6hdxA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with r3-hib-amp (see paper)
29% identity, 100% coverage: 2:435/435 of query aligns to 22:463/474 of 6hdxA
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (2~{R})-2-methyl-3-oxidanyl-propanoate: Y162 (≠ F140), G164 (= G142), S237 (≠ G212), G238 (≠ A213), E239 (= E214), P240 (= P215), C262 (≠ I235), G263 (≠ Y236), S264 (≠ G237), A266 (≠ S239), F271 (≠ G245), D331 (= D304), I353 (≠ M325), R356 (= R328), K453 (= K425)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: Y162 (≠ F140), G164 (= G142), S237 (≠ G212), G263 (≠ Y236), S264 (≠ G237), A266 (≠ S239), F271 (≠ G245)
6siyA Paak family amp-ligase with amp and substrate (see paper)
28% identity, 95% coverage: 15:428/435 of query aligns to 15:411/433 of 6siyA
- binding 3-hydroxyanthranilic acid: T125 (≠ Y135), P126 (≠ G136), T132 (≠ G142), L135 (≠ G145), R153 (≠ G163), N177 (≠ T183), A209 (= A213), E232 (≠ I235), G234 (= G237), S235 (≠ L238)
- binding adenosine monophosphate: S85 (= S93), A209 (= A213), E210 (= E214), P211 (= P215), E232 (≠ I235), Y233 (= Y236), G234 (= G237), S235 (≠ L238), T236 (≠ S239), D296 (= D304), V316 (≠ R324)
- binding magnesium ion: R75 (≠ M83), E76 (= E84), L78 (≠ I86), P117 (≠ K127), G144 (= G154), A145 (= A155), T146 (≠ S156)
6siwA Paak family amp-ligase with amp (see paper)
28% identity, 95% coverage: 15:428/435 of query aligns to 14:410/432 of 6siwA
- binding adenosine monophosphate: S84 (= S93), A208 (= A213), E209 (= E214), P210 (= P215), E231 (≠ I235), Y232 (= Y236), G233 (= G237), S234 (≠ L238), T235 (≠ S239), D295 (= D304), V315 (≠ R324)
- binding magnesium ion: E75 (= E84), L77 (≠ I86), S83 (= S92), P116 (≠ K127), G143 (= G154), T145 (≠ S156), E236 (= E240)
- binding zinc ion: C244 (= C250), H250 (= H258), C304 (= C313), C306 (= C315)
6sixB Paak family amp-ligase with anp (see paper)
28% identity, 95% coverage: 15:428/435 of query aligns to 19:415/437 of 6sixB
- binding phosphoaminophosphonic acid-adenylate ester: S88 (= S92), S89 (= S93), A213 (= A213), E214 (= E214), P215 (= P215), E236 (≠ I235), Y237 (= Y236), G238 (= G237), S239 (≠ L238), T240 (≠ S239), E241 (= E240), D300 (= D304), V320 (≠ R324), R323 (= R328)
- binding magnesium ion: R79 (≠ M83), E80 (= E84), P121 (≠ K127), T150 (≠ S156)
- binding zinc ion: C249 (= C250), H255 (= H258), C309 (= C313), C311 (= C315)
3cw8X 4-chlorobenzoyl-coa ligase/synthetase, bound to 4cba-adenylate (see paper)
25% identity, 66% coverage: 70:355/435 of query aligns to 133:427/501 of 3cw8X
- binding 5'-O-[(S)-{[(4-chlorophenyl)carbonyl]oxy}(hydroxy)phosphoryl]adenosine: H207 (≠ L139), V208 (≠ F140), V209 (≠ T141), G281 (≠ A213), A282 (≠ E214), T283 (≠ P215), I303 (= I235), Y304 (= Y236), G305 (= G237), T306 (≠ L238), T307 (≠ S239), M310 (= M242), N311 (≠ G243), M324 (≠ I259), D385 (= D304)
3cw9A 4-chlorobenzoyl-coa ligase/synthetase in the thioester-forming conformation, bound to 4-chlorophenacyl-coa (see paper)
25% identity, 66% coverage: 70:355/435 of query aligns to 133:427/503 of 3cw9A
- active site: T161 (≠ S92), R181 (≠ W112), H207 (≠ L139), T307 (≠ S239), E308 (= E240), I406 (= I334), N411 (= N339)
- binding 4-Chlorophenacyl-coenzyme A: M203 (≠ Y135), P204 (≠ G136), H207 (≠ L139), V208 (≠ F140), V209 (≠ T141), A280 (≠ G212), G305 (= G237), T306 (≠ L238), M310 (= M242), N311 (≠ G243), S407 (≠ I335), G408 (≠ R336), G409 (= G337), E410 (≠ V338)
- binding adenosine monophosphate: T161 (≠ S92), G281 (≠ A213), A282 (≠ E214), T283 (≠ P215), I303 (= I235), Y304 (= Y236), G305 (= G237), T306 (≠ L238), T307 (≠ S239), D385 (= D304), R400 (= R328), I406 (= I334), N411 (= N339)
Sites not aligning to the query:
6m2uA The crystal structure of benzoate coenzyme a ligase double mutant (h333a/i334a) in complex with 2-chloro-1,3-thiazole-5-carboxylate-amp (see paper)
26% identity, 63% coverage: 81:355/435 of query aligns to 157:443/518 of 6m2uA
- active site: S176 (= S92), T196 (≠ W112), T324 (≠ S239), E325 (= E240), K422 (≠ I334), Y427 (≠ N339)
- binding adenosine monophosphate: G298 (≠ S217), E299 (= E218), A300 (= A219), D319 (= D234), G320 (≠ I235), I321 (≠ Y236), G322 (= G237), T324 (≠ S239), D401 (≠ C315), R416 (= R328), K422 (≠ I334), Y427 (≠ N339)
- binding 2-chloranyl-1,3-thiazole-5-carboxylic acid: Y223 (= Y137), A297 (≠ W216), G322 (= G237), S323 (≠ L238), A328 (≠ G243)
Sites not aligning to the query:
6m2tA The crystal structure of benzoate coenzyme a ligase double mutant (h333a/i334a) in complex with 2-methyl-thiazole-5 carboxylate-amp
26% identity, 63% coverage: 81:355/435 of query aligns to 157:443/518 of 6m2tA
- active site: S176 (= S92), T196 (≠ W112), T324 (≠ S239), E325 (= E240), K422 (≠ I334), Y427 (≠ N339)
- binding 2-methyl-1,3-thiazole-5-carboxylic acid: Y223 (= Y137), G322 (= G237), S323 (≠ L238), A328 (≠ G243)
- binding adenosine monophosphate: G298 (≠ S217), E299 (= E218), A300 (= A219), G320 (≠ I235), I321 (≠ Y236), S323 (≠ L238), T324 (≠ S239), D401 (≠ C315), R416 (= R328), K422 (≠ I334), Y427 (≠ N339)
Sites not aligning to the query:
3dlpX 4-chlorobenzoyl-coa ligase/synthetase, mutant d402p, bound to 4cb (see paper)
24% identity, 66% coverage: 70:355/435 of query aligns to 133:427/504 of 3dlpX
5n9xB Structure of adenylation domain thr1 involved in the biosynthesis of 4-chlorothreonine in streptomyces sp.Oh-5093, ligand bound structure (see paper)
25% identity, 47% coverage: 84:287/435 of query aligns to 133:338/486 of 5n9xB
- active site: T141 (≠ S92), L161 (≠ W112), T286 (≠ S239), E287 (= E240)
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (2~{S},3~{R})-2-azanyl-3-oxidanyl-butanoate: T141 (≠ S92), D186 (≠ G136), F187 (≠ Y137), G257 (≠ A213), E258 (= E214), K259 (≠ P215), N281 (≠ D234), M282 (≠ I235), Y283 (= Y236), G284 (= G237), I285 (≠ L238), T286 (≠ S239), V290 (≠ G243)
Sites not aligning to the query:
- active site: 391, 396, 472
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (2~{S},3~{R})-2-azanyl-3-oxidanyl-butanoate: 370, 382, 385, 470, 472
5n9xA Structure of adenylation domain thr1 involved in the biosynthesis of 4-chlorothreonine in streptomyces sp.Oh-5093, ligand bound structure (see paper)
26% identity, 41% coverage: 63:240/435 of query aligns to 114:290/489 of 5n9xA
- active site: T142 (≠ S92), L162 (≠ W112), T289 (≠ S239), E290 (= E240)
- binding adenosine-5'-triphosphate: T142 (≠ S92), S143 (= S93), K150 (≠ T100), G260 (≠ A213), E261 (= E214), K262 (≠ P215), N284 (≠ D234), M285 (≠ I235), Y286 (= Y236), G287 (= G237), I288 (≠ L238), T289 (≠ S239)
- binding threonine: F186 (≠ Y135), D187 (≠ G136), F188 (≠ Y137), G260 (≠ A213), G287 (= G237)
Sites not aligning to the query:
1t5dX 4-chlorobenzoyl-coa ligase/synthetase bound to 4-chlorobenzoate (see paper)
24% identity, 66% coverage: 70:355/435 of query aligns to 133:425/502 of 1t5dX
Sites not aligning to the query:
Query Sequence
>WP_012469913.1 NCBI__GCF_000020385.1:WP_012469913.1
MANYFNEEFETLPRAALEALQLKRLQATVARVYANVPFYKQSFDAAGIKPEDIRSLTDLQ
RLPFTTKQNMRDSYPYGLFAAPMEEIVRIHASSGTTGKPTVVGYTQKDIETWSELMARSF
MAAGAHKGDIIHNAYGYGLFTGGLGAHYGAEKLGASVIPISGGNTKRQIMIMQDFGSTVL
TCTPSYSLYLAEEAAAEGVDIKSSKLRVGIFGAEPWSEAMRGEIEANLGLDAIDIYGLSE
IMGPGVAIECIEEKHGLHIWEDHFIPEIINPETGQPVAEGEKGELVITTITKQGIPLIRY
RTRDITSISYAPCACGRTHGRIARMSGRSDDMLIIRGVNVFPSQIESILVRIEGVEPHYL
LIVDRKDNLDTLEVQVEVDERIFSDEIKVLQTLARRIEKEIKDMLGVTCTVKLVEPKTIQ
RSEGKAQRVRDNRKL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory