SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012471074.1 NCBI__GCF_000020385.1:WP_012471074.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4dxvA Crystal structure of dihydrodipicolinate synthase from acinetobacter baumannii complexed with mg and cl ions at 1.80 a resolution
51% identity, 100% coverage: 2:290/290 of query aligns to 2:291/291 of 4dxvA

query
sites
4dxvA

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active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding magnesium ion: T104 (= T103), N134 (= N133), V135 (= V134), P136 (= P135), T139 (= T138)

3u8gA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with oxalic acid at 1.80 a resolution
51% identity, 100% coverage: 2:290/290 of query aligns to 2:291/291 of 3u8gA

query
sites
3u8gA

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active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding oxalate ion: A8 (= A8), G43 (= G42), T44 (= T43), T45 (= T44), L101 (= L100), Y133 (= Y132), K161 (= K160)

3tdfA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with 2-ketobutanoic acid at 1.99 a resolution
51% identity, 100% coverage: 2:290/290 of query aligns to 2:291/291 of 3tdfA

query
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3tdfA

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active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding 2-ketobutyric acid: A8 (= A8), T44 (= T43), T45 (= T44), Y133 (= Y132), K161 (= K160), I203 (= I202)

3tceA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with 5-hydroxylysine at 2.6 a resolution
51% identity, 100% coverage: 2:290/290 of query aligns to 2:291/291 of 3tceA

query
sites
3tceA

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active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding 5-hydroxylysine: S49 (= S48), L51 (= L50), H56 (= H55), Y106 (= Y105)

3rk8A Crystal structure of the chloride inhibited dihydrodipicolinate synthase from acinetobacter baumannii complexed with pyruvate at 1.8 a resolution
51% identity, 100% coverage: 2:290/290 of query aligns to 2:291/291 of 3rk8A

query
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3rk8A

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E

V

I

F

I

D

R

Q

V

A

A

K

N

G

K

R

R

V

I

P

P

V

I

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

S

T

T

A

R

E

E

A

A

I

I

H

E

L

L

T

T

R

K

E

A

A

A

K

K

E

D

I

L

G

G

V

A

A

D

G

A

V

A

L

L

V

V

T

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

T

T

Q

Q

E

E

G

G

L

L

Y

Y

L

Q

H

H

Y

Y

T

K

A

A

I

I

A

A

D

E

A

A

V

V

E

E

I

L

P

P

Q

L

I

I

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

G

V

V

N

D

L

L

L

S

P

N

E

D

T

T

V

A

A

V

R

R

L

L

A

A

G

E

H

I

K

P

N

N

I

I

V

V

A

G

I

I

K
|
K

E

D

A

A

T

T

G

G

S

D

L

V

Q

P

Q

R

A

G

S

K

E

A

V

L

M

I

A

D

L

A

C

L

G

N

D

G

Q

K

I

M

D

A

V

V

F

Y

S

S

G
|
G

D

D

F

E

I

T

T

A

F

W

P

E

M

L

M

M

A

L

C

L

G

G

A

A

K

D

G

G

V

N

I
|
I

S

S

V

V

T

T

A

A

N

N

I

I

M

A

P

P

K

K

A

A

I

M

G

S

D

E

L

V

T

C

D

A

A

V

F

A

Y

I

A

A

G

K

D

D

L

E

E

Q

K

Q

A

A

R

K

Q

T

L

L

H

N

L

N

D

K

T

I

L

A

K

N

I

L

S

H

N

N

A

I

M

L

F
|
F

I

C

E

E

S

S

N

N

P

P

V

I

P

P

V

V

K

K

T

W

A

A

L

L

A

H

L

E

M

M

G

G

K

L

C

I

S

D

D

T

E

G

V

I

R

R

L

L

P

P

L

L

A

T

P

P

M

L

S

A

A

E

A

Q

N

Y

K

R

A

E

K

P

L

L

E

R

A

N

I

A

M

L

K

K

E

D

Y

A

K

G

L

I

I

I

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding pyruvic acid: R138 (= R137), K161 (= K160), G186 (= G185), F244 (= F243)

3pueB Crystal structure of the complex of dhydrodipicolinate synthase from acinetobacter baumannii with lysine at 2.6a resolution
51% identity, 100% coverage: 2:290/290 of query aligns to 2:291/291 of 3pueB

query
sites
3pueB

F

I

K

Q

G

G

S

S

I

I

V

V

A

A

I

I

V

V

T

T

P

P

-

M

F

L

N

K

N

D

G

G

A

G

V

V

D

D

E

W

K

K

K

S

L

L

R

E

E

K

L

L

V

V

D

E

F

W

Q

H

I

I

E

E

N

Q

G

G

T

T

D

N

A

S

I

I

V

V

A

A

C

V

G

G

T
|
T

T

T

G

G

E

E

S

A

S

S

T

T

L

L

D

S

N

M

E

E

H

H

L

T

N

Q

V

V

I

I

K

K

I

E

V

I

F

I

D

R

Q

V

A

A

K

N

G

K

R

R

V

I

P

P

V

I

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

S

T

T

A

R

E

E

A

A

I

I

H

E

L

L

T

T

R

K

E

A

A

A

K

K

E

D

I

L

G

G

V

A

A

D

G

A

V

A

L

L

V

V

T

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

T

T

Q

Q

E

E

G

G

L

L

Y

Y

L

Q

H

H

Y

Y

T

K

A

A

I

I

A

A

D

E

A

A

V

V

E

E

I

L

P

P

Q

L

I

I

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

G

V

V

N

D

L

L

L

S

P

N

E

D

T

T

V

A

A

V

R

R

L

L

A

A

G

E

H

I

K

P

N

N

I

I

V

V

A

G

I

I

K
|
K

E

D

A

A

T

T

G

G

S

D

L

V

Q

P

Q

R

A

G

S

K

E

A

V

L

M

I

A

D

L

A

C

L

G

N

D

G

Q

K

I

M

D

A

V

V

F

Y

S

S

G

G

D

D

F

E

I

T

T

A

F

W

P

E

M

L

M

M

A

L

C

L

G

G

A

A

K

D

G

G

V

N

I
|
I

S

S

V

V

T

T

A

A

N

N

I

I

M

A

P

P

K

K

A

A

I

M

G

S

D

E

L

V

T

C

D

A

A

V

F

A

Y

I

A

A

G

K

D

D

L

E

E

Q

K

Q

A

A

R

K

Q

T

L

L

H

N

L

N

D

K

T

I

L

A

K

N

I

L

S

H

N

N

A

I

M

L

F
|
F

I

C

E

E

S

S

N
|
N

P

P

V

I

P

P

V

V

K

K

T

W

A

A

L

L

A

H

L

E

M

M

G

G

K

L

C

I

S

D

D

T

E

G

V

I

R

R

L

L

P

P

L

L

A

T

P

P

M

L

S

A

A

E

A

Q

N

Y

K

R

A

E

K

P

L

L

E

R

A

N

I

A

M

L

K

K

E

D

Y

A

K

G

L

I

I

I

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding lysine: R138 (= R137), F244 (= F243), N248 (= N247)

3s8hA Structure of dihydrodipicolinate synthase complexed with 3- hydroxypropanoic acid(hpa)at 2.70 a resolution
54% identity, 99% coverage: 1:286/290 of query aligns to 1:287/292 of 3s8hA

query
sites
3s8hA

M

M

F

I

K

A

G

G

S

S

I

M

V

V

A
|
A

I
x
L

V

V

T

T

P

P

F

F

N

D

-

A

N

Q

G

G

A

R

V

L

D

D

E

W

K

D

K

S

L

L

R

A

E

K

L

L

V

V

D

D

F

F

Q

H

I

L

E

Q

N

D

G

G

T

T

D

N

A

A

I

I

V
|
V

A

A

C

V

G
|
G

T
|
T

G
|
G

E

E

S

S

S

A

T

T

L

L

D

D

N

V

E

E

H

H

L

I

N

Q

V

V

I

V

K

R

I

R

V

V

F

V

D

D

Q

Q

A

V

K

K

G

G

R

R

V

I

P

P

V

V

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

S

T

T

A

R

E

E

A

A

I

V

H

A

L

L

T

T

R

E

E

A

A

A

K

K

E

S

I

G

G

G

V

A

A

D

G

A

V

C

L

L

V

V

T

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

T

T

Q

Q

E

E

G

G

L

M

Y

Y

L

Q

H

H

Y

F

T

R

A

H

I

I

A

A

D

E

A

A

V

V

E

A

I

I

P

P

Q

Q

I

I

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

S

V

C

N

D

L

M

L

L

P

P

E

E

T

T

V

V

A

E

R

R

L

L

A

S

G

K

H

V

K

P

N

N

I

I

V

I

A

G

I

I

K
|
K

E

E

A

A

T

T

G

G

S

D

L

L

Q

Q

Q

R

A

A

S

K

E

E

V

V

M

I

A

E

L

R

C

V

G

G

D

K

Q

D

I

F

D

L

V

V

F

Y

S

S

G

G

D

D

F

A

I

T

T

A

F

V

P

E

M

L

M

M

A

L

C

L

G

G

A

G

K

K

G

G

V

N

I
|
I

S

S

V

V

T

T

A

A

N

N

I

V

M

A

P

P

K

R

A

A

I

M

G

S

D

D

L

L

T

C

D

A

A

A

F

A

Y

M

A

R

G

G

D

D

L

A

E

A

K

A

A

A

R

R

Q

A

L

I

H

N

L

D

D

R

T

L

L

M

K

P

I

L

S

H

N

K

A

A

M

L

F

F

I

I

E

E

S

S

N

N

P

P

V

I

P

P

V

V

K

K

T

W

A

A

L

L

A

H

L

E

M

M

G

G

K

L

C

I

S

P

D

E

E

G

V

I

R

R

L

L

P

P

L

L

A

T

P

W

M

L

S

S

A

P

A

H

N

C

K

H

A

D

K

P

L

L

E

R

A

Q

I

A

M

M

K

R

E

Q

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding 3-hydroxy-propanoic acid: A8 (= A8), L9 (≠ I9), V40 (= V39), G43 (= G42), T44 (= T43), T45 (= T44), G46 (= G45), K161 (= K160)

3puoA Crystal structure of dihydrodipicolinate synthase from pseudomonas aeruginosa(psdhdps)complexed with l-lysine at 2.65a resolution (see paper)
54% identity, 99% coverage: 1:286/290 of query aligns to 1:287/292 of 3puoA

query
sites
3puoA

M

M

F

I

K

A

G

G

S

S

I

M

V

V

A

A

I

L

V

V

T

T

P

P

F

F

N

D

-

A

N

Q

G

G

A

R

V

L

D

D

E

W

K

D

K

S

L

L

R

A

E

K

L

L

V

V

D

D

F

F

Q

H

I

L

E

Q

N

D

G

G

T

T

D

N

A

A

I

I

V

V

A

A

C

V

G

G

T
|
T

T

T

G

G

E

E

S
|
S

S
x
A

T

T

L
|
L

D
|
D

N
x
V

E

E

H

H

L

I

N

Q

V

V

I

V

K

R

I

R

V

V

F

V

D

D

Q

Q

A

V

K

K

G

G

R

R

V

I

P

P

V

V

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

S

T

T

A

R

E

E

A

A

I

V

H

A

L

L

T

T

R

E

E

A

A

A

K

K

E

S

I

G

G

G

V

A

A

D

G

A

V

C

L

L

V

V

T

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

T

T

Q

Q

E

E

G

G

L

M

Y

Y

L

Q

H

H

Y

F

T

R

A

H

I

I

A

A

D

E

A

A

V

V

E

A

I

I

P

P

Q

Q

I

I

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

S

V

C

N

D

L

M

L

L

P

P

E

E

T

T

V

V

A

E

R

R

L

L

A

S

G

K

H

V

K

P

N

N

I

I

V

I

A

G

I

I

K
|
K

E

E

A

A

T

T

G

G

S

D

L

L

Q

Q

Q

R

A

A

S

K

E

E

V

V

M

I

A

E

L

R

C

V

G

G

D

K

Q

D

I

F

D

L

V

V

F

Y

S

S

G

G

D

D

F

A

I

T

T

A

F

V

P

E

M

L

M

M

A

L

C

L

G

G

A

G

K

K

G

G

V

N

I
|
I

S

S

V

V

T

T

A

A

N

N

I

V

M

A

P

P

K

R

A

A

I

M

G

S

D

D

L

L

T

C

D

A

A

A

F

A

Y

M

A

R

G

G

D

D

L

A

E

A

K

A

A

A

R

R

Q

A

L

I

H

N

L

D

D

R

T

L

L

M

K

P

I

L

S

H

N

K

A

A

M

L

F

F

I

I

E

E

S

S

N

N

P

P

V

I

P

P

V

V

K

K

T

W

A

A

L

L

A

H

L

E

M

M

G

G

K

L

C

I

S

P

D

E

E

G

V
x
I

R

R

L
|
L

P

P

L
|
L

A
x
T

P

W

M

L

S

S

A

P

A

H

N

C

K

H

A

D

K

P

L

L

E

R

A

Q

I

A

M

M

K

R

E

Q

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding lysine: S48 (= S47), A49 (≠ S48), L51 (= L50), D52 (= D51), V53 (≠ N52), I266 (≠ V265), L268 (= L267), L270 (= L269), T271 (≠ A270)

2atsA Dihydrodipicolinate synthase co-crystallised with (s)-lysine
47% identity, 100% coverage: 1:290/290 of query aligns to 1:292/292 of 2atsA

query
sites
2atsA

M

M

F

F

K

T

G

G

S

S

I

I

V

V

A

A

I

I

V

V

T

T

P

P

F

M

N

D

-

E

N

K

G

G

A

N

V

V

D

C

E

R

K

A

K

S

L

L

R

K

E

K

L

L

V

I

D

D

F

Y

Q

H

I

V

E

A

N

S

G

G

T

T

D

S

A

A

I

I

V

V

A

S

C

V

G

G

T
|
T

T

T

G

G

E

E

S

S

S
x
A

T

T

L
|
L

D

N

N
x
H

E

D

E

E

H
|
H

L

A

N

D

V

V

I

V

K

M

I

M

V

T

F

L

D

D

Q

L

A

A

K

D

G

G

R

R

V

I

P

P

V

V

I

I

A

A

G

G

T

T

G

G

S

A

N
|
N

S

A

T

T

A

A

E
|
E

A

A

I

I

H

S

L

L

T

T

R

Q

E

R

A

F

K

N

E

D

I

S

G

G

V

I

A

V

G

G

V

C

L

L

L

T

V

V

T

T

P

P

Y
|
Y

N

N

K

R

P

P

T

S

Q

Q

E

E

G

G

L

L

Y

Y

L

Q

H

H

Y

F

T

K

A

A

I

I

A

A

D

E

A

H

V

T

E

D

I

L

P

P

Q

Q

I

I

L

L

Y
|
Y

N

N

V

V

P

P

G

S

R
|
R

T

T

G

G

V

C

N

D

L

L

P

P

E

E

T

T

V

V

A

G

R

R

L

L

A

A

G

K

H

V

K

K

N

N

I

I

V

I

A

G

I

I

K
|
K

E

E

A

A

T

T

G

G

S

N

L

L

Q

T

Q

R

A

V

S

N

E

Q

V

I

M

K

A

E

L

L

C

V

G

S

D

D

Q

D

I

F

D

V

V

L

F

L

S

S

G

G

D

D

F

A

I

S

T

A

F

L

P

D

M

F

M

M

A

Q

C

L

G

G

A

G

K

H

G

G

V

V

I
|
I

S

S

V

V

T

T

A

A

N

N

I

V

M

A

P

A

K

R

A

D

I

M

G

A

D

Q

L

M

T

C

D

K

A

L

F

A

Y

A

A

E

G

G

D

H

L

F

E

A

K

E

A

A

R

R

Q

V

L

I

H

N

L

Q

D

R

T

L

L

M

K

P

I

L

S

H

N

N

A

K

M

L

F

F

I

V

E

E

S

P

N

N

P

P

V

I

P

P

V

V

K

K

T

W

A

A

L

C

A

K

L

E

M

L

G

G

-

L

K

V

C

A

S

T

D

D

E

T

V

L

R

R

L

L

P

P

L

M

A

T

P

P

M

I

S

T

A

D

A

S

N

G

K

R

A

E

K

T

L

V

E

R

A

A

I

A

M

L

K

K

E

H

Y

A

K

G

L

L

I

L

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding d-lysine: A49 (≠ S48), L51 (= L50), H53 (≠ N52), H56 (= H55), N80 (= N79), E84 (= E83), Y106 (= Y105)

P0A6L2 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 from Escherichia coli (strain K12) (see 7 papers)
47% identity, 100% coverage: 1:290/290 of query aligns to 1:292/292 of P0A6L2

query
sites
P0A6L2

M

M

F

F

K

T

G

G

S

S

I

I

V

V

A

A

I

I

V

V

T

T

P

P

F

M

N

D

-

E

N

K

G

G

A

N

V

V

D

C

E

R

K

A

K

S

L

L

R

K

E

K

L

L

V

I

D

D

F

Y

Q

H

I

V

E

A

N

S

G

G

T

T

D

S

A

A

I

I

V

V

A

S

C

V

G

G

T
|
T

G

G

E

E

S

S

S

A

T

T

L

L

D

N

N

H

E

D

E

E

H

H

L

A

N

D

V

V

I

V

K

M

I

M

V

T

F

L

D

D

Q

L

A

A

K

D

G

G

R

R

V

I

P

P

V

V

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

A

T

T

A

A

E

E

A

A

I

I

H

S

L

L

T

T

R

Q

E

R

A

F

K

N

E

D

I

S

G

G

V

I

A

V

G

G

V

C

L

L

L

T

V

V

T

T

P

P

Y

Y

Y
|
Y

N

N

K

R

P

P

T

S

Q

Q

E

E

G

G

L

L

Y

Y

L

Q

H

H

Y

F

T

K

A

A

I

I

A

A

D

E

A

H

V

T

E

D

I

L

P

P

Q

Q

I

I

L

L

Y
|
Y

N

N

V

V

P

P

G

S

R
|
R

T

T

G

G

V

C

N

D

L

L

P

P

E

E

T

T

V

V

A

G

R

R

L

L

A

A

G

K

H

V

K

K

N

N

I

I

V

I

A

G

I

I

K
|
K

E

E

A

A

T

T

G

G

S

N

L

L

Q

T

Q

R

A

V

S

N

E

Q

V

I

M

K

A

E

L

L

C

V

G

S

D

D

Q

D

I

F

D

V

V

L

F

L

S

S

G

G

D

D

F

A

I

S

T

A

F

L

P

D

M

F

M

M

A

Q

C
x
L

G

G

A

G

K

H

G

G

V

V

I
|
I

S

S

V

V

T

T

A

A

N

N

I

V

M

A

P

A

K

R

A

D

I

M

G

A

D

Q

L

M

T

C

D

K

A

L

F

A

Y

A

A

E

G

G

D

H

L

F

E

A

K

E

A

A

R

R

Q

V

L

I

H

N

L

Q

D

R

T

L

L

M

K

P

I

L

S

H

N

N

A

K

M

L

F

F

I

V

E

E

S

P

N

N

P

P

V

I

P

P

V

V

K

K

T

W

A

A

L

C

A

K

L

E

M

L

G

G

-

L

K

V

C

A

S

T

D

D

E

T

V

L

R

R

L

L

P

P

L

M

A

T

P

P

M

I

S

T

A

D

A

S

N

G

K

R

A

E

K

T

L

V

E

R

A

A

I

A

M

L

K

K

E

H

Y

A

K

G

L

L

I

L

T44 (= T43) mutation to S: 8% of wild-type activity. 4-fold decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA.; mutation to V: Reduced kcat by 99.9%.
T45 (= T44) binding pyruvate
Y107 (= Y106) mutation to F: Reduced kcat by 90%.; mutation to W: Reduced activity by 95%. Reduced affinity for both substrates. Exists as a mixture of monomer, dimer and tetramer in solution. Has significantly lower thermal stability than the wild-type enzyme.
Y133 (= Y132) mutation to F: Reduced kcat by 99.7%. Reduced affinity for both substrates.
R138 (= R137) mutation R->A,H: Strongly increased KM for L-aspartate 4-semialdehyde. No effect on KM for pyruvate. Reduced activity by 99.7%.
K161 (= K160) mutation to A: 0.1% of wild-type activity. 3-fold decrease in affinity for pyruvate, and 2-fold decrease in that for (S)-ASA.; mutation to R: 0.35% of wild-type activity. 3-fold decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA.
L197 (≠ C196) mutation L->Y,D: 1.4 to 2.5% of wild-type activity. Decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA. Exists as a dimer in solution.
I203 (= I202) binding pyruvate

5t25A Kinetic, spectral and structural characterization of the slow binding inhibitor acetopyruvate with dihydrodipicolinate synthase from escherichia coli.
47% identity, 100% coverage: 1:290/290 of query aligns to 2:293/293 of 5t25A

query
sites
5t25A

M

M

F

F

K

T

G

G

S

S

I

I

V

V

A

A

I

I

V

V

T

T

P

P

F

M

N

D

-

E

N

K

G

G

A

N

V

V

D

C

E

R

K

A

K

S

L

L

R

K

E

K

L

L

V

I

D

D

F

Y

Q

H

I

V

E

A

N

S

G

G

T

T

D

S

A

A

I

I

V

V

A

S

C

V

G

G

T
|
T

T

T

G

G

E

E

S

S

S
x
A

T

T

L
|
L

D

N

N
x
H

E

D

E

E

H

H

L

A

N

D

V

V

I

V

K

M

I

M

V

T

F

L

D

D

Q

L

A

A

K

D

G

G

R

R

V

I

P

P

V

V

I

I

A

A

G

G

T

T

G

G

S

A

N
|
N

S

A

T

T

A

A

E
|
E

A

A

I

I

H

S

L

L

T

T

R

Q

E

R

A

F

K

N

E

D

I

S

G

G

V

I

A

V

G

G

V

C

L

L

L

T

V

V

T

T

P

P

Y
|
Y

N

N

K

R

P

P

T

S

Q

Q

E

E

G

G

L

L

Y

Y

L

Q

H

H

Y

F

T

K

A

A

I

I

A

A

D

E

A

H

V

T

E

D

I

L

P

P

Q

Q

I

I

L

L

Y
|
Y

N

N

V

V

P

P

G

S

R
|
R

T

T

G

G

V

C

N

D

L

L

P

P

E

E

T

T

V

V

A

G

R

R

L

L

A

A

G

K

H

V

K

K

N

N

I

I

V

I

A

G

I

I

K
|
K

E

E

A

A

T

T

G

G

S

N

L

L

Q

T

Q

R

A

V

S

N

E

Q

V

I

M

K

A

E

L

L

C

V

G

S

D

D

Q

D

I

F

D

V

V

L

F

L

S

S

G

G

D

D

F

A

I

S

T

A

F

L

P

D

M

F

M

M

A

Q

C

L

G

G

A

G

K

H

G

G

V

V

I
|
I

S

S

V

V

T

T

A

A

N

N

I

V

M

A

P

A

K

R

A

D

I

M

G

A

D

Q

L

M

T

C

D

K

A

L

F

A

Y

A

A

E

G

G

D

H

L

F

E

A

K

E

A

A

R

R

Q

V

L

I

H

N

L

Q

D

R

T

L

L

M

K

P

I

L

S

H

N

N

A

K

M

L

F

F

I

V

E

E

S

P

N

N

P

P

V

I

P

P

V

V

K

K

T

W

A

A

L

C

A

K

L

E

M

L

G

G

-

L

K

V

C

A

S

T

D

D

E

T

V

L

R

R

L

L

P

P

L

M

A

T

P

P

M

I

S

T

A

D

A

S

N

G

K

R

A

E

K

T

L

V

E

R

A

A

I

A

M

L

K

K

E

H

Y

A

K

G

L

L

I

L

active site: T45 (= T43), Y108 (= Y106), Y134 (= Y132), R139 (= R137), K162 (= K160), I204 (= I202)
binding lysine: A50 (≠ S48), L52 (= L50), H54 (≠ N52), N81 (= N79), E85 (= E83), Y107 (= Y105)

3i7sA Dihydrodipicolinate synthase mutant - k161a - with the substrate pyruvate bound in the active site. (see paper)
47% identity, 100% coverage: 1:290/290 of query aligns to 1:292/292 of 3i7sA

query
sites
3i7sA

M

M

F

F

K

T

G

G

S

S

I

I

V

V

A
|
A

I

I

V

V

T

T

P

P

F

M

N

D

-

E

N

K

G

G

A

N

V

V

D

C

E

R

K

A

K

S

L

L

R

K

E

K

L

L

V

I

D

D

F

Y

Q

H

I

V

E

A

N

S

G

G

T

T

D

S

A

A

I

I

V

V

A

S

C

V

G

G

T
|
T

G

G

E

E

S

S

S

A

T

T

L

L

D

N

N

H

E

D

E

E

H

H

L

A

N

D

V

V

I

V

K

M

I

M

V

T

F

L

D

D

Q

L

A

A

K

D

G

G

R

R

V

I

P

P

V

V

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

A

T

T

A

A

E

E

A

A

I

I

H

S

L

L

T

T

R

Q

E

R

A

F

K

N

E

D

I

S

G

G

V

I

A

V

G

G

V

C

L

L

L

T

V

V

T

T

P

P

Y

Y

Y
|
Y

N

N

K

R

P

P

T

S

Q

Q

E

E

G

G

L

L

Y

Y

L

Q

H

H

Y

F

T

K

A

A

I

I

A

A

D

E

A

H

V

T

E

D

I

L

P

P

Q

Q

I

I

L

L

Y
|
Y

N

N

V

V

P

P

G

S

R
|
R

T

T

G

G

V

C

N

D

L

L

P

P

E

E

T

T

V

V

A

G

R

R

L

L

A

A

G

K

H

V

K

K

N

N

I

I

V

I

A

G

I

I

K
x
R

E

E

A

A

T

T

G

G

S

N

L

L

Q

T

Q

R

A

V

S

N

E

Q

V

I

M

K

A

E

L

L

C

V

G

S

D

D

Q

D

I

F

D

V

V

L

F

L

S

S

G

G

D

D

F

A

I

S

T

A

F

L

P

D

M

F

M

M

A

Q

C

L

G

G

A

G

K

H

G

G

V

V

I
|
I

S

S

V

V

T

T

A

A

N

N

I

V

M

A

P

A

K

R

A

D

I

M

G

A

D

Q

L

M

T

C

D

K

A

L

F

A

Y

A

A

E

G

G

D

H

L

F

E

A

K

E

A

A

R

R

Q

V

L

I

H

N

L

Q

D

R

T

L

L

M

K

P

I

L

S

H

N

N

A

K

M

L

F

F

I

V

E

E

S

P

N

N

P

P

V

I

P

P

V

V

K

K

T

W

A

A

L

C

A

K

L

E

M

L

G

G

-

L

K

V

C

A

S

T

D

D

E

T

V

L

R

R

L

L

P

P

L

M

A

T

P

P

M

I

S

T

A

D

A

S

N

G

K

R

A

E

K

T

L

V

E

R

A

A

I

A

M

L

K

K

E

H

Y

A

K

G

L

L

I

L

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), R161 (≠ K160), I203 (= I202)
binding pyruvic acid: A8 (= A8), T44 (= T43), T45 (= T44), Y133 (= Y132)

Q9X1K9 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
45% identity, 100% coverage: 1:290/290 of query aligns to 1:294/294 of Q9X1K9

query
sites
Q9X1K9

M

M

F

F

K

R

G

G

S

V

I

G

V

T

A

A

I

I

V

V

T

T

P

P

F

F

N

K

N

N

G

G

A

E

V

L

D

D

E

L

K

E

K

S

L

Y

R

E

E

R

L

L

V

V

D

R

F

Y

Q

Q

I

L

E

E

N

N

G

G

T

V

D

N

A

A

I

L

V

I

A

V

C

L

G

G

T

T

G

G

E

E

S

S

S

P

T

T

L

V

D

N

N

E

E

D

E

E

H

R

L

E

N

K

V

L

I

V

K

S

I

R

V

T

F

L

D

E

Q

I

A

V

K

D

G

G

R

K

V

I

P

P

V

V

I

I

A

V

G

G

T

A

G

G

S

T

N

N

S

S

T

T

A

E

E

K

A

T

I

L

H

K

L

L

T

V

R

K

E

Q

A

A

K

E

E

K

I

L

G

G

V

A

A

N

G

G

V

V

L

L

L

V

V

V

T

T

P

P

Y

Y

N

N

K

K

P

P

T

T

Q

Q

E

E

G

G

L

L

Y

Y

L

Q

H

H

Y

Y

T

K

A

Y

I

I

A

S

D

E

A

R

V

T

E

D

I

L

P

G

Q

I

I

V

L

V

Y

Y

N

N

V

V

P

P

G

G

R

R

T

T

G

G

V

V

N

N

L

V

L

L

P

P

E

E

T

T

V

A

A

A

R

R

L

I

A

A

G

A

H

D

-

L

K

K

N

N

I

V

V

V

A

G

I

I

K

K

E

E

A

A

T

N

G

P

S
x
D

L
x
I

Q
x
D

Q

Q

A

I

S

D

E

R

V

T

M

V

A

S

L

L

C

T

G

K

D

Q

-

A

-

R

-

S

Q

D

I

F

D

M

V

V

F

W

S

S

G

G

D

N

D

D

F

D

I

R

T

T

F

F

P

Y

M

L

A

C

C

A

G

G

A

G

K

D

G

G

V

V

I

I

S

S

V

V

T

V

A

S

N

N

I

V

M

A

P

P

K

K

A

Q

I

M

G

V

D

E

L

L

T

C

D

A

A

E

F

Y

Y

F

A

S

G

G

D

N

L

L

E

E

K

K

A

S

R

R

Q

E

L

V

H

H

L

R

D

K

T

L

L

R

K

P

I

L

S

M

N

K

A

A

M

L

F

F

I

V

E

E

S

T

N

N

P

P

V

I

P

P

V

V

K

K

T

A

A

A

L

L

A

N

L

L

M

M

G

G

K

F

C

I

S

E

D

N

E

E

V

L

R

R

L

L

P

P

L

L

A

V

P

P

M

A

S

S

A

E

A

K

N

T

K

V

A

E

K

L

L

L

E

R

A

N

I

V

M

L

K

K

E

E

Y

S

K

G

L

L

I

L

DID 166:168 (≠ SLQ 165:167) mutation to AAA: Exists as a monomer in solution. Decreased activity and substrate affinity. Reduced thermal stability.

1o5kA Crystal structure of dihydrodipicolinate synthase (tm1521) from thermotoga maritima at 1.80 a resolution
45% identity, 100% coverage: 1:290/290 of query aligns to 2:295/295 of 1o5kA

query
sites
1o5kA

M

M

F

F

K

R

G

G

S

V

I

G

V

T

A

A

I

I

V

V

T

T

P

P

F

F

N

K

N

N

G

G

A

E

V

L

D

D

E

L

K

E

K

S

L

Y

R

E

E

R

L

L

V

V

D

R

F

Y

Q

Q

I

L

E

E

N

N

G

G

T

V

D

N

A

A

I

L

V

I

A

V

C

L

G

G

T
|
T

T

T

G

G

E

E

S

S

S

P

T

T

L

V

D

N

N

E

E

D

E

E

H

R

L

E

N

K

V

L

I

V

K

S

I

R

V

T

F

L

D
x
E

Q

I

A

V

K
x
D

G

G

R

K

V

I

P

P

V

V

I

I

A

V

G

G

T

A

G

G

S

T

N

N

S

S

T

T

A

E

E

K

A

T

I

L

H

K

L

L

T

V

R

K

E

Q

A

A

K

E

E

K

I

L

G

G

V

A

A

N

G

G

V

V

L

L

L

V

V

V

T

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

T

T

Q

Q

E

E

G

G

L

L

Y

Y

L

Q

H

H

Y

Y

T

K

A

Y

I

I

A

S

D

E

A

R

V

T

E

D

I

L

P

G

Q

I

I

V

L

V

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

G

V

V

N

N

L

V

L

L

P

P

E

E

T

T

V

A

A

A

R

R

L

I

A

A

G

A

H

D

-

L

K

K

N

N

I

V

V

V

A

G

I

I

K
|
K

E

E

A

A

T

N

G

P

S

D

L

I

Q

D

Q

Q

A

I

S

D

E

R

V

T

M

V

A

S

L

L

C

T

G

K

D

Q

-

A

-

R

-

S

Q

D

I

F

D

M

V

V

F

W

S

S

G

G

D

N

D

D

F

D

I

R

T

T

F

F

P

Y

M

L

A

C

C

A

G

G

A

G

K

D

G

G

V

V

I
|
I

S

S

V

V

T

V

A

S

N

N

I

V

M

A

P

P

K

K

A

Q

I

M

G

V

D

E

L

L

T

C

D

A

A

E

F

Y

Y

F

A

S

G

G

D

N

L

L

E

E

K

K

A

S

R

R

Q

E

L

V

H

H

L

R

D

K

T

L

L

R

K

P

I

L

S

M

N

K

A

A

M

L

F

F

I

V

E

E

S

T

N

N

P

P

V

I

P

P

V

V

K

K

T

A

A

A

L

L

A

N

L

L

M

M

G

G

K

F

C

I

S

E

D

N

E

E

V

L

R

R

L

L

P

P

L

L

A

V

P

P

M

A

S

S

A

E

A

K

N

T

K

V

A

E

K

L

L

L

E

R

A

N

I

V

M

L

K

K

E

E

Y

S

K

G

L

L

I

L

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K162 (= K160), I207 (= I202)
binding calcium ion: E65 (≠ D64), D68 (≠ K67)

4pfmA Shewanella benthica dhdps with lysine and pyruvate
47% identity, 100% coverage: 1:289/290 of query aligns to 2:292/295 of 4pfmA

query
sites
4pfmA

M

M

F

I

K

N

G

G

S

S

I

I

V

V

A

A

I

L

V

I

T

T

P

P

F

L

N

N

-

S

N

D

G

G

A

T

V

V

D

D

E

Y

K

T

K

S

L

L

R

E

E

K

L

L

V

V

D

E

F

Y

Q

H

I

I

E

T

N

E

G

G

T

T

D

D

A

A

I

I

V

V

A

A

C

V

G

G

T
|
T

T

T

G

G

E

E

S
|
S

S
x
A

T

T

L
|
L

D

P

N

I

E

S

E

E

H
|
H

L

I

N

A

V

V

I

V

K

G

I

Q

V

T

F

V

D

K

Q

F

A

A

K

S

G

G

R

R

V

I

P

P

V

V

I

I

A

G

G

G

T

N

G

G

S

A

N
|
N

S

A

T

T

A

A

E
|
E

A

A

I

I

H

E

L

L

T

T

R

K

E

A

A

Q

K

N

E

K

I

L

G

G

V

V

A

A

G

A

V

M

L

L

L

G

V

V

T

T

P

P

Y
|
Y

N

N

K

K

P

P

T

S

Q

P

E

K

G

G

L

L

Y

I

L

A

H

H

Y

Y

T

T

A

A

I

V

A

A

D

A

A

S

V

T

E

D

I

I

P

P

Q

Q

I

I

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

A

V

V

N

D

L

M

L

L

P

P

E

E

T

T

V

I

A

A

R

Q

L

L

A

V

G

E

H

V

K

P

N

N

I

I

V

I

A

G

I

V

K
|
K

E

D

A

A

T

T

G

G

S

D

L

V

Q

A

Q

R

A

V

S

K

E

Q

V

L

M

R

A

D

L

L

C

C

G

G

D

N

Q

D

I

F

D

L

V

L

F

Y

S

S

G

G

D

D

F

A

I

T

T

A

F

R

P

E

M

F

M

L

A

T

C

L

G

G

A

G

K

D

G

G

V

V

I
|
I

S

S

V

V

T

A

A

N

N

N

I

I

M

V

P

P

K

K

A

L

I

F

G

K

D

L

L

M

T

C

D

D

A

A

F

A

Y

L

A

A

G

G

D

D

L

T

E

Q

K

A

A

A

R

M

Q

A

L

A

H

E

L

D

D

Q

T

I

L

K

K

G

I

L

S

F

N

S

A

A

M

L

F

F

I

C

E

E

S

A

N

N

P

P

V

I

P

P

V

V

K

K

T

W

A

A

L

A

A

H

L

K

M

M

G

G

K

L

C

I

S

S

D

Q

-

G

E

D

V

I

R

R

L

L

P

P

L

L

A

T

P

E

M

L

S

S

A

T

A

E

N

F

K

H

A

G

K

L

L

L

E

L

A

D

I

A

M

M

K

K

E

N

Y

A

K

R

L

I

active site: T45 (= T43), Y108 (= Y106), Y134 (= Y132), R139 (= R137), K162 (= K160), I204 (= I202)
binding lysine: S49 (= S47), A50 (≠ S48), L52 (= L50), H57 (= H55), N81 (= N79), E85 (= E83), Y107 (= Y105)

4m19A Dihydrodipicolinate synthase from c. Jejuni with pyruvate bound to the active site and lysine bound to allosteric site (see paper)
47% identity, 99% coverage: 4:289/290 of query aligns to 6:293/296 of 4m19A

query
sites
4m19A

G

G

S

A

I

M

V

T

A

A

I

L

V

I

T

T

P

P

F

F

N

K

N

N

G

G

A

K

V

V

D

D

E

E

K

Q

K

S

L

Y

R

A

E

R

L

L

V

I

D

K

F

R

Q

Q

I

I

E

E

N

N

G

G

T

I

D

D

A

A

I

V

V

V

A

P

C

V

G

G

T
|
T

T

T

G

G

E

E

S
|
S

S
x
A

T

T

L
|
L

D

T

N
x
H

E

E

H
|
H

L

R

N

T

V

C

I

I

K

E

I

I

V

A

F

V

D

E

Q

T

A

C

K

K

G

G

-

T

R

K

V

V

P

K

V

V

I

L

A

A

G

G

T

A

G

G

S

S

N
|
N

S

A

T

T

A

H

E
|
E

A

A

I

V

H

G

L

L

T

A

R

K

E

F

A

A

K

K

E

E

I

H

G

G

V

A

A

D

G

G

V

I

L

L

L

S

V

V

T

A

P

P

Y
|
Y

N

N

K

K

P

P

T

T

Q

Q

E

Q

G

G

L

L

Y

Y

L

E

H

H

Y

Y

T

K

A

A

I

I

A

A

D

Q

A

S

V

V

E

D

I

I

P

P

Q

V

I

L

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

G

V

C

N

E

L

I

L

S

P

T

E

D

T

T

V

I

A

I

R

K

L

L

-

F

A

R

G

D

H

C

K

E

N

N

I

I

V

Y

A

G

I

V

K
|
K

E

E

A

A

T

S

G

G

S

N

L

I

Q

D

Q

K

A

C

S

V

E

D

V

L

M

L

A

A

L

H

C

-

G

E

D

P

Q

R

I

M

D

M

V

L

F

I

S

S

G

G

D

E

D

D

F

A

I

I

T

N

F

Y

P

P

M

I

M

L

A

S

C

N

G

G

A

G

K

K

G

G

V

V

I
|
I

S

S

V

V

T

T

A

S

N

N

I

L

M

L

P

P

K

D

A

M

I

I

G

S

D

A

L

L

T

T

D

H

A

F

F

A

Y

L

A

D

G

E

D

N

L

Y

E

K

K

E

A

A

R

K

Q

K

L

I

H

N

L

D

D

E

T

L

L

Y

K

N

I

I

S

N

N

K

A

I

M

L

F

F

I

C

E

E

S

S

N

N

P

P

V

I

P

P

V

I

K

K

T

T

A

A

L

M

A

Y

L

L

M

A

G

G

K

L

C

I

-

E

S

S

D

L

E

E

V

F

R

R

L

L

P

P

L

L

A

C

P

S

M

P

S

S

A

K

A

E

N

N

K

F

A

A

K

K

L

I

E

E

A

E

I

V

M

M

K

K

E

K

Y

Y

K

K

L

I

active site: T45 (= T43), Y109 (= Y106), Y135 (= Y132), R140 (= R137), K164 (= K160), I205 (= I202)
binding lysine: S49 (= S47), A50 (≠ S48), L52 (= L50), H54 (≠ N52), H57 (= H55), N82 (= N79), E86 (= E83), Y108 (= Y105)

6u01B Dihydrodipicolinate synthase (dhdps) from c.Jejuni, n84d mutant with pyruvate bound in the active site (see paper)
46% identity, 99% coverage: 4:289/290 of query aligns to 6:293/296 of 6u01B

query
sites
6u01B

G

G

S

A

I

M

V

T

A

A

I

L

V

I

T

T

P

P

F

F

N

K

N

N

G

G

A

K

V

V

D

D

E

E

K

Q

K

S

L

Y

R

A

E

R

L

L

V

I

D

K

F

R

Q

Q

I

I

E

E

N

N

G

G

T

I

D

D

A

A

I

V

V

V

A

P

C

V

G

G

T
|
T

T

T

G

G

E

E

S

S

S

A

T

T

L

L

D

T

N

H

E

E

H

H

L

R

N

T

V

C

I

I

K

E

I

I

V

A

F

V

D

E

Q

T

A

C

K

K

G

G

-

T

R

K

V

V

P

K

V

V

I

L

A

A

G

G

T

A

G

G

S

S

N

D

S

A

T

T

A

H

E

E

A

A

I

V

H

G

L

L

T

A

R

K

E

F

A

A

K

K

E

E

I

H

G

G

V

A

A

D

G

G

V

I

L

L

L

S

V

V

T

A

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

T

T

Q

Q

E

Q

G

G

L

L

Y

Y

L

E

H

H

Y

Y

T

K

A

A

I

I

A

A

D

Q

A

S

V

V

E

D

I

I

P

P

Q

V

I

L

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

G

V

C

N

E

L

I

L

S

P

T

E

D

T

T

V

I

A

I

R

K

L

L

-

F

A

R

G

D

H

C

K

E

N

N

I

I

V

Y

A

G

I

V

K
|
K

E

E

A

A

T

S

G

G

S

N

L

I

Q

D

Q

K

A

C

S

V

E

D

V

L

M

L

A

A

L

H

C

-

G

E

D

P

Q

R

I

M

D

M

V

L

F

I

S
|
S

G

G

D
x
E

D
|
D

F

A

I

I

T
x
N

F

Y

P

P

M

I

M

L

A

S

C

N

G

G

A

G

K

K

G

G

V

V

I
|
I

S

S

V

V

T

T

A

S

N

N

I

L

M

L

P

P

K

D

A

M

I

I

G

S

D

A

L

L

T

T

D

H

A

F

F

A

Y

L

A

D

G

E

D

N

L

Y

E

K

K

E

A

A

R

K

Q

K

L

I

H

N

L

D

D

E

T

L

L

Y

K

N

I

I

S

N

N

K

A

I

M

L

F

F

I

C

E

E

S

S

N

N

P

P

V

I

P

P

V

I

K

K

T

T

A

A

L

M

A

Y

L

L

M

A

G

G

K

L

C

I

-

E

S

S

D

L

E

E

V

F

R

R

L

L

P

P

L

L

A

C

P

S

M

P

S

S

A

K

A

E

N

N

K

F

A

A

K

K

L

I

E

E

A

E

I

V

M

M

K

K

E

K

Y

Y

K

K

L

I

active site: T45 (= T43), Y109 (= Y106), Y135 (= Y132), R140 (= R137), K164 (= K160), I205 (= I202)
binding magnesium ion: S187 (= S184), E189 (≠ D186), D190 (= D187), N193 (≠ T190), I205 (= I202)

5ktlA Dihydrodipicolinate synthase from the industrial and evolutionarily important cyanobacteria anabaena variabilis. (see paper)
46% identity, 100% coverage: 2:290/290 of query aligns to 5:295/295 of 5ktlA

query
sites
5ktlA

F

F

K

G

G

T

S

V

I

L

V

T

A

A

I

M

V

I

T

T

P

P

F

F

N

K

-

A

N

D

G

G

A

S

V

V

D

N

E

Y

K

A

K

V

L

A

R

A

E

E

L

L

V

A

D

A

F

H

Q

L

I

V

E

D

N

N

G

G

T

T

D

D

A

T

I

L

V

V

A

V

C

C

G

G

T
|
T

T

T

G

G

E

E

S
|
S

S

P

T

T

L
|
L

D

S

N

W

E

D

E

E

H
x
E

L

Y

N

N

V

L

I

F

K

V

I
x
E

V

V

F

L

D
x
Q

Q

T

A

V

K

A

G

G

R

K

V

A

P

K

V

V

I

I

A

A

G

G

T

C

G

G

S

S

N

N

S

S

T

T

A

K

E

E

A

A

I

I

H

A

L

A

T

T

R

Q

E

K

A

A

K

A

E

K

I

I

G

G

V

V

A

H

G

G

V

T

L

L

L

Q

V

V

T

V

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

T

P

Q

Q

E

A

G

G

L

L

Y

Y

L

Q

H

H

Y

F

T

Q

A

A

I

I

A

A

D

Q

A

A

V

C

-

P

E

D

I

L

P

P

Q

L

I

L

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

G

V

Q

N

N

L

L

L

S

P

P

E

E

T

T

V

V

A

V

R

R

L

L

A

A

G

E

H

I

K

D

N

N

I

I

V

I

A

G

I

V

K
|
K

E

E

A

A

T

S

G

G

S

N

L

L

Q

D

Q

Q

A

A

S

G

E

E

V

I

M

R

A

R

L

S

C

T

G

P

D

K

Q

E

I

F

D

Q

V

I

F

Y

S

A

G

G

D

D

F

S

I

L

T

T

F

L

P

P

M

L

A

A

C

I

G

G

A

A

K

K

G

G

V

V

I
x
V

S

S

V

V

T

A

A

S

N

H

I

L

M

V

P

G

K

N

A

Q

I

L

G

Q

D

Q

L

M

T

I

D

Q

A

A

F

F

Y

N

A

S

G

G

D

Q

L

V

E

T

K

V

A

A

R

S

Q

D

L

I

H

H

L

L

D

R

T

L

L

L

K

P

I

L

S

F

N

K

A

A

M

L

F

F

I

I

E

T

S

T

N

N

P

P

V

I

P

P

V

I

K

K

T

Q

A

A

L

L

A

K

L

L

M

Q

G

G

K

W

C

E

S

V

D

G

E

S

V

T

R

R

L

P

P

P

L

L

A

S

P

D

M

A

S

D

A

A

A

E

N

V

K

S

A

Q

K

K

L

L

E

E

A

A

I

V

M

M

K

K

E

H

Y

L

K

N

L

L

I

I

active site: T47 (= T43), Y110 (= Y106), Y137 (= Y132), R142 (= R137), K165 (= K160), V207 (≠ I202)
binding manganese (ii) ion: S51 (= S47), L54 (= L50), E59 (≠ H55), E65 (≠ I61), Q68 (≠ D64)

7kkdB Dihydrodipicolinate synthase (dhdps) from c.Jejuni, n84a mutant with pyruvate bound in the active site and r,r-bislysine bound at the allosteric site
46% identity, 99% coverage: 4:289/290 of query aligns to 16:303/306 of 7kkdB

query
sites
7kkdB

G

G

S

A

I

M

V

T

A

A

I

L

V

I

T

T

P

P

F

F

N

K

N

N

G

G

A

K

V

V

D

D

E

E

K

Q

K

S

L

Y

R

A

E

R

L

L

V

I

D

K

F

R

Q

Q

I

I

E

E

N

N

G

G

T

I

D

D

A

A

I

V

V

V

A

P

C

V

G

G

T

T

G

G

E

E

S
|
S

S

A

T

T

L
|
L

D

T

N

H

E

E

H
|
H

L

R

N

T

V

C

I

I

K

E

I

I

V

A

F

V

D

E

Q

T

A

C

K

K

G

G

-

T

R

K

V

V

P

K

V

V

I

L

A

A

G

G

T

A

G

G

S

S

N

A

S

A

T

T

A

H

E
|
E

A

A

I

V

H

G

L

L

T

A

R

K

E

F

A

A

K

K

E

E

I

H

G

G

V

A

A

D

G

G

V

I

L

L

L

S

V

V

T

A

P

P

Y
|
Y

Y

Y

N

N

K

K

P

P

T

T

Q

Q

E

Q

G

G

L

L

Y

Y

L

E

H

H

Y

Y

T

K

A

A

I

I

A

A

D

Q

A

S

V

V

E

D

I

I

P

P

Q

V

I

L

L

L

Y

Y

N

N

V

V

P

P

G

G

R

R

T

T

G

G

V

C

N

E

L

I

L

S

P

T

E

D

T

T

V

I

A

I

R

K

L

L

-

F

A

R

G

D

H

C

K

E

N

N

I

I

V

Y

A

G

I

V

K

K

E

E

A

A

T

S

G

G

S

N

L

I

Q

D

Q

K

A

C

S

V

E

D

V

L

M

L

A

A

L

H

C

-

G

E

D

P

Q

R

I

M

D

M

V

L

F

I

S

S

G

G

D

E

D

D

F

A

I

I

T

N

F

Y

P

P

M

I

M

L

A

S

C

N

G

G

A

G

K

K

G

G

V

V

I

I

S

S

V

V

T

T

A

S

N

N

I

L

M

L

P

P

K

D

A

M

I

I

G

S

D

A

L

L

T

T

D

H

A

F

F

A

Y

L

A

D

G

E

D

N

L

Y

E

K

K

E

A

A

R

K

Q

K

L

I

H

N

L

D

D

E

T

L

L

Y

K

N

I

I

S

N

N

K

A

I

M

L

F

F

I

C

E

E

S

S

N

N

P

P

V

I

P

P

V

I

K

K

T

T

A

A

L

M

A

Y

L

L

M

A

G

G

K

L

C

I

-

E

S

S

D

L

E

E

V

F

R

R

L

L

P

P

L

L

A

C

P

S

M

P

S

S

A

K

A

E

N

N

K

F

A

A

K

K

L

I

E

E

A

E

I

V

M

M

K

K

E

K

Y

Y

K

K

L

I

binding (2R,5R)-2,5-diamino-2,5-bis(4-aminobutyl)hexanedioic acid: S59 (= S47), L62 (= L50), H67 (= H55), E96 (= E83), Y118 (= Y105)

7kg2A Dihydrodipicolinate synthase (dhdps) from c.Jejuni, h59k mutant with pyruvate bound in the active site and l-histidine bound at the allosteric site
46% identity, 99% coverage: 4:289/290 of query aligns to 6:293/296 of 7kg2A

query
sites
7kg2A

G

G

S

A

I

M

V

T

A

A

I

L

V

I

T

T

P

P

F

F

N

K

N

N

G

G

A

K

V

V

D

D

E

E

K

Q

K

S

L

Y

R

A

E

R

L

L

V

I

D

K

F

R

Q

Q

I

I

E

E

N

N

G

G

T

I

D

D

A

A

I

V

V

V

A

P

C

V

G

G

T

T

G

G

E

E

S
|
S

S
x
A

T

T

L
|
L

D

T

N

H

E

E

H
x
K

L

R

N

T

V

C

I

I

K

E

I

I

V

A

F

V

D

E

Q

T

A

C

K

K

G

G

-

T

R

K

V

V

P

K

V

V

I

L

A

A

G

G

T

A

G

G

S

S

N
|
N

S

A

T

T

A

H

E
|
E

A

A

I

V

H

G

L

L

T

A

R

K

E

F

A

A

K

K

E

E

I

H

G

G

V

A

A

D

G

G

V

I

L

L

L

S

V

V

T

A

P

P

Y
|
Y

Y

Y

N

N

K

K

P

P

T

T

Q

Q

E

Q

G

G

L

L

Y

Y

L

E

H

H

Y

Y

T

K

A

A

I

I

A

A

D

Q

A

S

V

V

E

D

I

I

P

P

Q

V

I

L

L

L

Y

Y

N

N

V

V

P

P

G

G

R

R

T

T

G

G

V

C

N

E

L

I

L

S

P

T

E

D

T

T

V

I

A

I

R

K

L

L

-

F

A

R

G

D

H

C

K

E

N

N

I

I

V

Y

A

G

I

V

K

K

E

E

A

A

T

S

G

G

S

N

L

I

Q

D

Q

K

A

C

S

V

E

D

V

L

M

L

A

A

L

H

C

-

G

E

D

P

Q

R

I

M

D

M

V

L

F

I

S

S

G

G

D

E

D

D

F

A

I

I

T

N

F

Y

P

P

M

I

M

L

A

S

C

N

G

G

A

G

K

K

G

G

V

V

I

I

S

S

V

V

T

T

A

S

N

N

I

L

M

L

P

P

K

D

A

M

I

I

G

S

D

A

L

L

T

T

D

H

A

F

F

A

Y

L

A

D

G

E

D

N

L

Y

E

K

K

E

A

A

R

K

Q

K

L

I

H

N

L

D

D

E

T

L

L

Y

K

N

I

I

S

N

N

K

A

I

M

L

F

F

I

C

E

E

S

S

N

N

P

P

V

I

P

P

V

I

K

K

T

T

A

A

L

M

A

Y

L

L

M

A

G

G

K

L

C

I

-

E

S

S

D

L

E

E

V

F

R

R

L

L

P

P

L

L

A

C

P

S

M

P

S

S

A

K

A

E

N

N

K

F

A

A

K

K

L

I

E

E

A

E

I

V

M

M

K

K

E

K

Y

Y

K

K

L

I

binding histidine: S49 (= S47), A50 (≠ S48), L52 (= L50), K57 (≠ H55), N82 (= N79), E86 (= E83), Y108 (= Y105)

Query Sequence

>WP_012471074.1 NCBI__GCF_000020385.1:WP_012471074.1
MFKGSIVAIVTPFNNGAVDEKKLRELVDFQIENGTDAIVACGTTGESSTLDNEEHLNVIK
IVFDQAKGRVPVIAGTGSNSTAEAIHLTREAKEIGVAGVLLVTPYYNKPTQEGLYLHYTA
IADAVEIPQILYNVPGRTGVNLLPETVARLAGHKNIVAIKEATGSLQQASEVMALCGDQI
DVFSGDDFITFPMMACGAKGVISVTANIMPKAIGDLTDAFYAGDLEKARQLHLDTLKISN
AMFIESNPVPVKTALALMGKCSDEVRLPLAPMSAANKAKLEAIMKEYKLI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory