SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012501363.1 NCBI__GCF_000020505.1:WP_012501363.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 6 hits to proteins with known functional sites (download)

5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
26% identity, 32% coverage: 426:636/662 of query aligns to 5:241/291 of 5odqB

query
sites
5odqB

Y

F

F

F

T

L

G
|
G

C
|
C

Y

I

A

M

S

P

H

N

D

-

P

-

R

R

N

Y

R

A

K

G

V

V

A

E

E

A

S

A

F

T

V

R

R

T

I

V

C

M

E

E

A

K

A

L

G

G

V

V

T

E

V

L

A

V

I

D

L

M

G

T

G

G

A

A

E

S

R

-

C
|
C

G

P

E

A

P

P

A
x
G

R

V

K

-

L

F

G

G

-

S

-

F

N

D

E

Q

Y

K

L

T

Y

W

R

L

M

T

V

L

A

A

D

A

S

R

N

N

I

L

E

C

A

I

I

A

R

E

A

E

S

M

G

G

A

V

R

D

R

-

I

I

V

V

T

T

A

V

C
|
C

P

N

H
x
G

C
|
C

F

Y

N

G

T

S

L

L

-

F

-

E

-

A

-

H

-

L

-

H

-

D

-

N

-

K

-

E

A

A

R

L

D

N

Y

F

R

V

E

N

L

E

G

K

L

L

D

D

-

K

-

V

-

G

-

K

-

E

-

Y

-

K

-

G

-

N

L

V

P

K

V

V

E

R

H

H

H

F

S

A

T

E

F

L

I

I

A

Y

R

N

L

D

I

I

N

G

D

V

G

D

K

K

L

I

K

A

L

E

K

K

P

V

E

E

R

R

-

P

-

L

-

N

F

I

T

N

A

V

T

G

F

V

H

H

D

Y

S
x
G

C
|
C

Y
x
H

L

F

A

L

R

K

Y

P

Q

T

E

D

I

V

-

K

-

H

-

L

-

G

-

S

A

A

E

E

E

R

P

P

-

V

-

M

-

L

R

D

E

E

V

I

V

V

R

E

A

A

A

T

G

G

I

A

K

K

L

S

V

V

E

-

M

-

A

-

A

P

F

Y

G

A

K

D

N

K

G

M

F

M

C
|
C

G

G

A

A

G
|
G

G

G

R

V

I
x
R

L

A

A

R

E

E

E

L

K

E

L

L

G

S

T

L

P

D

I

M

V

T

D

N

E

E

R

K

L

I

R

E

Q

N

A

M

R

I

Q

K

T

A

G

G

A

A

K

D

S

C

L

T

V

V

S

N

A

V

C
|
C

P

P

F
|
F

C
|
C

L

H

S
x
L

M

Q

F

F

E

D

D

R

G

G

binding Non-cubane [4Fe-4S]-cluster: G8 (= G429), C9 (= C430), C41 (= C465), C42 (= C466), C78 (= C502), G80 (≠ H504), C81 (= C505), G152 (≠ S552), C153 (= C553), H154 (≠ Y554), C193 (= C588), C194 (= C589), C231 (= C626), F233 (= F628), C234 (= C629)
binding 2-bromanylethanesulfonic acid: G46 (≠ A470), G80 (≠ H504), H154 (≠ Y554), G197 (= G592), R201 (≠ I596), F233 (= F628), L236 (≠ S631)

5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
26% identity, 32% coverage: 426:636/662 of query aligns to 5:241/291 of 5odhH

query
sites
5odhH

Y

F

F

F

T

L

G
|
G

C
|
C

Y

I

A

M

S

P

H

N

D

-

P

-

R

R

N

Y

R

A

K

G

V

V

A

E

E

A

S

A

F

T

V

R

R

T

I

V

C

M

E

E

A

K

A

L

G

G

V

V

T

E

V

L

A

V

I

D

L

M

G

T

G

G

A

A

E

S

R

-

C
|
C

G

P

E
x
A

P
|
P

A
x
G

R

V

K

-

L

F

G

G

-

S

-

F

N

D

E

Q

Y

K

L

T

Y

W

R

L

M

T

V

L

A

A

D

A

S

R

N

N

I

L

E

C

A

I

I

A

R

E

A

E

S

M

G

G

A

V

R

D

R

-

I

I

V

V

T

T

A

V

C
|
C

P

N

H
x
G

C
|
C

F

Y

N

G

T

S

L

L

-

F

-

E

-

A

-

H

-

L

-

H

-

D

-

N

-

K

-

E

A

A

R

L

D

N

Y

F

R

V

E

N

L

E

G

K

L

L

D

D

-

K

-

V

-

G

-

K

-

E

-

Y

-

K

-

G

-

N

L

V

P

K

V

V

E

R

H

H

H

F

S

A

T

E

F

L

I

I

A

Y

R

N

L

D

I

I

N

G

D

V

G

D

K

K

L

I

K

A

L

E

K

K

P

V

E

E

R

R

-

P

-

L

-

N

F

I

T

N

A

V

T

G

F

V

H

H

D

Y

S

G

C
|
C

Y
x
H

L

F

A

L

R

K

Y

P

Q

T

E

D

I

V

-

K

-

H

-

L

-

G

-

S

A

A

E

E

E

R

P

P

-

V

-

M

-

L

R

D

E

E

V

I

V

V

R

E

A

A

A

T

G

G

I

A

K

K

L

S

V

V

E

-

M

-

A

-

A

P

F

Y

G

A

K

D

N

K

G

M

F

M

C
|
C

G

G

A

A

G
|
G

G

G

R

V

I
x
R

L

A

A

R

E

E

E

L

K

E

L

L

G

S

T

L

P

D

I

M

V

T

D

N

E

E

R

K

L

I

R

E

Q

N

A

M

R

I

Q

K

T

A

G

G

A

A

K

D

S

C

L

T

V

V

S

N

A

V

C
|
C

P

P

F
|
F

C
|
C

L

H

S

L

M

Q

F

F

E

D

D
x
R

G

G

binding Non-cubane [4Fe-4S]-cluster: G8 (= G429), C9 (= C430), C41 (= C465), C42 (= C466), C78 (= C502), G80 (≠ H504), C81 (= C505), C153 (= C553), H154 (≠ Y554), C193 (= C588), C194 (= C589), C231 (= C626), F233 (= F628), C234 (= C629)
binding 1-thioethanesulfonic acid: A44 (≠ E468), P45 (= P469), G46 (≠ A470), G80 (≠ H504), H154 (≠ Y554), G197 (= G592), G198 (= G593)
binding Coenzyme B: R201 (≠ I596), F233 (= F628), R240 (≠ D635)

5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
26% identity, 32% coverage: 426:636/662 of query aligns to 5:241/291 of 5odhB

query
sites
5odhB

Y

F

F

F

T

L

G
|
G

C
|
C

Y
x
I

A

M

S

P

H

N

D

-

P

-

R

R

N

Y

R

A

K

G

V

V

A

E

E

A

S

A

F

T

V

R

R

T

I

V

C

M

E

E

A

K

A

L

G

G

V

V

T

E

V

L

A

V

I

D

L

M

G

T

G

G

A

A

E

S

R

-

C
|
C

G

P

E

A

P
|
P

A
x
G

R

V

K

-

L

F

G

G

-

S

-

F

N

D

E

Q

Y

K

L

T

Y

W

R

L

M

T

V

L

A

A

D

A

S

R

N

N

I

L

E

C

A

I

I

A

R

E

A

E

S

M

G

G

A

V

R

D

R

-

I

I

V

V

T

T

A

V

C
|
C

P

N

H
x
G

C
|
C

F

Y

N

G

T

S

L

L

-

F

-

E

-

A

-

H

-

L

-

H

-

D

-

N

-

K

-

E

A

A

R

L

D

N

Y

F

R

V

E

N

L

E

G

K

L

L

D

D

-

K

-

V

-

G

-

K

-

E

-

Y

-

K

-

G

-

N

L

V

P

K

V

V

E

R

H

H

H

F

S

A

T

E

F

L

I

I

A

Y

R

N

L

D

I

I

N

G

D

V

G

D

K

K

L

I

K

A

L

E

K

K

P

V

E

E

R

R

-

P

-

L

-

N

F

I

T

N

A

V

T

G

F

V

H

H

D

Y

S
x
G

C
|
C

Y
x
H

L

F

A

L

R

K

Y

P

Q

T

E

D

I

V

-

K

-

H

-

L

-

G

-

S

A

A

E

E

E

R

P

P

-

V

-

M

-

L

R

D

E

E

V

I

V

V

R

E

A

A

A

T

G

G

I

A

K

K

L

S

V

V

E

-

M

-

A

-

A

P

F

Y

G

A

K

D

N

K

G

M

F

M

C
|
C

G

G

A

A

G

G

R

V

I

R

L

A

A

R

E

E

E

L

K

E

L

L

G

S

T

L

P

D

I

M

V

T

D

N

E

E

R

K

L

I

R

E

Q

N

A

M

R

I

Q

K

T

A

G

G

A

A

K

D

S

C

L

T

V

V

S

N

A

V

C
|
C

P

P

F
|
F

C
|
C

L

H

S

L

M

Q

F

F

E

D

D

R

G

G

binding Non-cubane [4Fe-4S]-cluster: G8 (= G429), C9 (= C430), I10 (≠ Y431), C41 (= C465), C42 (= C466), C78 (= C502), G80 (≠ H504), C81 (= C505), G152 (≠ S552), C153 (= C553), H154 (≠ Y554), C193 (= C588), C194 (= C589), C231 (= C626), F233 (= F628), C234 (= C629)
binding 1-thioethanesulfonic acid: P45 (= P469), G46 (≠ A470), G80 (≠ H504), F233 (= F628)

5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
26% identity, 32% coverage: 426:636/662 of query aligns to 5:241/291 of 5odcB

query
sites
5odcB

Y

F

F

F

T

L

G
|
G

C
|
C

Y
x
I

A

M

S

P

H

N

D

-

P

-

R

R

N

Y

R

A

K

G

V

V

A

E

E

A

S

A

F

T

V

R

R

T

I

V

C

M

E

E

A

K

A

L

G

G

V

V

T

E

V

L

A

V

I

D

L

M

G

T

G

G

A

A

E

S

R

-

C
|
C

G

P

E

A

P

P

A

G

R

V

K

-

L

F

G

G

-

S

-

F

N

D

E

Q

Y

K

L

T

Y

W

R

L

M

T

V

L

A

A

D

A

S

R

N

N

I

L

E

C

A

I

I

A

R

E

A

E

S

M

G

G

A

V

R

D

R

-

I

I

V

V

T

T

A

V

C
|
C

P
x
N

H
x
G

C
|
C

F

Y

N

G

T

S

L

L

-

F

-

E

-

A

-

H

-

L

-

H

-

D

-

N

-

K

-

E

A

A

R

L

D

N

Y

F

R

V

E

N

L

E

G

K

L

L

D

D

-

K

-

V

-

G

-

K

-

E

-

Y

-

K

-

G

-

N

L

V

P

K

V

V

E

R

H

H

H

F

S

A

T

E

F

L

I

I

A

Y

R

N

L

D

I

I

N

G

D

V

G

D

K

K

L

I

K

A

L

E

K

K

P

V

E

E

R

R

-

P

-

L

-

N

F

I

T

N

A

V

T

G

F

V

H

H

D

Y

S
x
G

C
|
C

Y

H

L

F

A

L

R

K

Y

P

Q

T

E

D

I

V

-

K

-

H

-

L

-

G

-

S

A

A

E

E

E

R

P

P

-

V

-

M

-

L

R

D

E

E

V

I

V

V

R

E

A

A

A

T

G

G

I

A

K

K

L

S

V

V

E

-

M

-

A

-

A

P

F

Y

G

A

K

D

N

K

G

M

F

M

C
|
C

G

G

A

A

G
|
G

G

G

R

V

I

R

L

A

A

R

E

E

E

L

K

E

L

L

G

S

T

L

P

D

I

M

V

T

D

N

E

E

R

K

L

I

R

E

Q

N

A

M

R

I

Q

K

T

A

G

G

A

A

K

D

S

C

L

T

V

V

S

N

A

V

C
|
C

P

P

F
|
F

C
|
C

L

H

S

L

M

Q

F

F

E

D

D

R

G

G

binding Non-cubane [4Fe-4S]-cluster: G8 (= G429), C9 (= C430), I10 (≠ Y431), C41 (= C465), C42 (= C466), C78 (= C502), N79 (≠ P503), G80 (≠ H504), C81 (= C505), G152 (≠ S552), C153 (= C553), C193 (= C588), C194 (= C589), G197 (= G592), C231 (= C626), F233 (= F628), C234 (= C629)

5odcI Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
32% identity, 16% coverage: 274:376/662 of query aligns to 24:108/173 of 5odcI

query
sites
5odcI

G

G

A

D

S

D

V

V

S

S

D

S

L

L

S

K

W

-

K

-

D

-

L

-

F

-

D

-

T

-

D

-

A

A

C
|
C

M
x
Y

Q
|
Q

C
|
C

G
|
G

R

T

C
|
C

Q

T

Q

G

R

S

C
|
C

P
|
P

A

-

H

-

L

-

T

S

G

G

K

R

P

R

L

T

S

S

-
x
Y

-
x
R

P
x
T

M

R

K

K

V

V

I

I

T

-

D

-

I

-

G

-

E

-

L

-

A

-

G

-

N

R

E

K

N

A

G

L

K

L

G

G

L

M

V

D

E

D

K

V

V

L

S

D

R

S

D

D

A
x
D

L

I

W

W

S

K

C
|
C

T
|
T

C
|
C

R
x
Y

A

T

C
|
C

E

Y

Q

E

I

R

C
|
C

P

P

A

R

S

D

I
x
V

E

K

H

-

I

V

G

T

K

E

I

I

E

K

M

T

R

I

R

R

S

N

L

L

A

A

L

A

M

Q

E

K

G

G

binding magnesium ion: R53 (vs. gap), D73 (≠ A340)
binding iron/sulfur cluster: C34 (= C292), Y35 (≠ M293), Q36 (= Q294), C37 (= C295), G38 (= G296), C40 (= C298), C44 (= C302), P45 (= P303), Y52 (vs. gap), T54 (≠ P313), C77 (= C344), T78 (= T345), T79 (= T346), C80 (= C347), Y81 (≠ R348), C83 (= C350), C87 (= C354), V91 (≠ I358)

5odcC Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
32% identity, 16% coverage: 274:378/662 of query aligns to 24:110/184 of 5odcC

query
sites
5odcC

G

G

A

D

S

D

V

V

S

S

D

S

L

L

S

K

W

-

K

-

D

-

L

-

F

-

D

-

T

-

D

-

A

A

C
|
C

M
x
Y

Q
|
Q

C
|
C

G
|
G

R

T

C
|
C

Q

T

Q

G

R

S

C
|
C

P
|
P

A

S

H

-

L

-

T

-

G

G

K

R

P

R

L

T

S

S

-
x
Y

-

R

P
x
T

M

R

K

K

V

V

I

I

T

-

D

-

I

-

G

-

E

-

L

-

A

-

G

-

N

R

E

K

N

A

G

L

K

L

G

G

L

M

V

D

E

D

K

V

V

L

S

D

R

S

D

D

A

D

L

I

W

W

S

K

C
|
C

T
|
T

C
|
C

R
x
Y

A

T

C
|
C

E

Y

Q

E

I

R

C
|
C

P
|
P

A

R

S

D

I
x
V

E

K

H

-

I

V

G

T

K

E

I

I

E

K

M

T

R

I

R

R

S

N

L

L

A

A

L

A

M

Q

E

K

G

G

E

N

F

M

binding iron/sulfur cluster: C34 (= C292), Y35 (≠ M293), Q36 (= Q294), C37 (= C295), G38 (= G296), C40 (= C298), C44 (= C302), P45 (= P303), Y52 (vs. gap), T54 (≠ P313), C77 (= C344), T78 (= T345), T79 (= T346), C80 (= C347), Y81 (≠ R348), C83 (= C350), C87 (= C354), P88 (= P355), V91 (≠ I358)

Query Sequence

>WP_012501363.1 NCBI__GCF_000020505.1:WP_012501363.1
MEATREIYWNIDHGTVALMYLMALAAVAVMVYGFRERIALWQKGKSLDRFDHLQQRLAHF
ITDALSQRKVLNVMQGGLPHALLFWGFLLLFIGTVLVMLQADLLAPLFSVNLLSGDFYRL
YSFVLDLAGLAAMLALAALAVRRFIIKPAGLETSSADVAIHLLLFAILFSGFLIEGARMA
VTEVRDNPALAAWSPVGAVFARPFLGMDEHLVRGAHKGFWLLHLLFGLGFLAVIPRTKLR
HLATTSGGSLLEPHEPTGTYSAIDLEDEHIEQFGASVVSDLSWKDLFDTDACMQCGRCQQ
RCPAHLTGKPLSPMKVITDIGELAGNENGKGLVEKVSRDALWSCTTCRACEQICPASIEH
IGKIIEMRRSLALMEGEFPGDEARRACEAIEVNGNPFGLSATSRGDWAKGLPVSIIKGKC
DADILYFTGCYASHDPRNRKVAESFVRICEAAGVTVAILGGAERCCGEPARKLGNEYLYR
MVADSNIEAIRASGARRIVTACPHCFNTLARDYRELGLDLPVEHHSTFIARLINDGKLKL
KPERFTATFHDSCYLARYQEIAEEPREVVRAAGIKLVEMAAFGKNGFCCGAGGGRILAEE
KLGTPIVDERLRQARQTGAKSLVSACPFCLSMFEDGLKRADSREPIKAYDLAEIVARSID
RK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory