SitesBLAST
Comparing WP_012501543.1 NCBI__GCF_000020505.1:WP_012501543.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
52% identity, 97% coverage: 6:337/341 of query aligns to 4:340/346 of Q04797
- S98 (= S100) modified: Phosphoserine
- Y146 (= Y152) modified: Phosphotyrosine
P23247 Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
55% identity, 97% coverage: 7:338/341 of query aligns to 5:333/337 of P23247
- C132 (= C136) active site, Acyl-thioester intermediate
2r00C Crystal structure of aspartate semialdehyde dehydrogenase ii complexed with asa from vibrio cholerae (see paper)
55% identity, 97% coverage: 7:338/341 of query aligns to 4:332/336 of 2r00C
4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/357 of 4r54A
- binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G77), S73 (≠ G78), T94 (≠ S99), S95 (= S100), R98 (= R103), K222 (= K219)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), T10 (= T15), G11 (= G16), A12 (≠ L17), V13 (= V18), A35 (= A40), S36 (= S41), R38 (= R43), S39 (= S44), T56 (≠ P61), S70 (= S75), A71 (= A76), G72 (= G77), T75 (≠ A80), N93 (= N98), T94 (≠ S99), N126 (= N135), C127 (= C136), G160 (= G169), G328 (= G325)
4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/357 of 4r41A
- binding 4-nitro-2-phosphonobenzoic acid: S70 (= S75), G72 (= G77), S73 (≠ G78), N93 (= N98), T94 (≠ S99), S95 (= S100), R98 (= R103), K222 (= K219)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), T10 (= T15), G11 (= G16), A12 (≠ L17), A35 (= A40), S36 (= S41), R38 (= R43), S39 (= S44), T56 (≠ P61), A71 (= A76), G160 (= G169), M161 (≠ K170), G162 (≠ A171)
4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/357 of 4r3nA
- active site: C127 (= C136), Q154 (= Q163), R244 (= R241), H251 (= H248)
- binding benzene-1,2,3-tricarboxylic acid: S73 (≠ G78), T94 (≠ S99), S95 (= S100), R98 (= R103), N126 (= N135), K222 (= K219)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), T10 (= T15), G11 (= G16), A12 (≠ L17), V13 (= V18), A35 (= A40), S36 (= S41), S39 (= S44), T56 (≠ P61), S70 (= S75), A71 (= A76), G72 (= G77), N93 (= N98), T94 (≠ S99), N126 (= N135), C127 (= C136), G160 (= G169), M161 (≠ K170), G328 (= G325)
3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/357 of 3q1lA
3q11A Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with NADP and aspartyl beta- difluorophosphonate (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/358 of 3q11A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), T10 (= T15), G11 (= G16), A35 (= A40), S36 (= S41), R38 (= R43), S39 (= S44), T56 (≠ P61), A71 (= A76), T75 (≠ A80), G160 (= G169), M161 (≠ K170), G162 (≠ A171)
- binding 5,5-difluoro-4-oxo-5-phosphono-D-norvaline: R98 (= R103), N126 (= N135), C127 (= C136), Q154 (= Q163), G158 (= G167), E219 (= E216), K222 (= K219), R244 (= R241)
3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/357 of 3pwsA
- binding (2R)-2-aminohexanedioic acid: R98 (= R103), N126 (= N135), G158 (= G167), I208 (= I205), E219 (= E216), K222 (= K219), R244 (= R241)
- binding adenosine-2'-5'-diphosphate: G8 (= G13), T10 (= T15), G11 (= G16), A12 (≠ L17), A35 (= A40), S36 (= S41), R38 (= R43), S39 (= S44), T56 (≠ P61), A71 (= A76), T75 (≠ A80), G160 (= G169), M161 (≠ K170)
3pwkA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/357 of 3pwkA
- binding 5'-o-monophosphoryladenylyl(2'->5')adenylyl(2'->5')adenosine: G8 (= G13), T10 (= T15), G11 (= G16), A12 (≠ L17), A35 (= A40), S36 (= S41), R38 (= R43), S39 (= S44), T56 (≠ P61), A71 (= A76), T75 (≠ A80), G160 (= G169)
- binding trans-cyclohexane-1,4-dicarboxylic acid: R98 (= R103), N126 (= N135), G158 (= G167), A159 (≠ K168), E219 (= E216), K222 (= K219), R244 (= R241)
2gz3A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP and aspartate- semialdehyde (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/357 of 2gz3A
- active site: C127 (= C136), Q154 (= Q163), R244 (= R241), H251 (= H248)
- binding (2r)-2-amino-4-oxobutanoic acid: C127 (= C136), Q154 (= Q163), G158 (= G167), E219 (= E216), R244 (= R241), H251 (= H248)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), T10 (= T15), G11 (= G16), A12 (≠ L17), V13 (= V18), A35 (= A40), S36 (= S41), R38 (= R43), S39 (= S44), T56 (≠ P61), S70 (= S75), A71 (= A76), G72 (= G77), T75 (≠ A80), N93 (= N98), G158 (= G167), G160 (= G169), M161 (≠ K170), N324 (= N321), A329 (= A326)
2gz2A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with 2',5'-adp (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/357 of 2gz2A
- active site: C127 (= C136), Q154 (= Q163), R244 (= R241), H251 (= H248)
- binding adenosine-2'-5'-diphosphate: G8 (= G13), T10 (= T15), G11 (= G16), A35 (= A40), S36 (= S41), R38 (= R43), S39 (= S44), T56 (≠ P61), A71 (= A76), T75 (≠ A80)
2gz1A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/357 of 2gz1A
- active site: C127 (= C136), Q154 (= Q163), R244 (= R241), H251 (= H248)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), T10 (= T15), G11 (= G16), A12 (≠ L17), V13 (= V18), A35 (= A40), S36 (= S41), R38 (= R43), S39 (= S44), T56 (≠ P61), S70 (= S75), A71 (= A76), G72 (= G77), T75 (≠ A80), N93 (= N98), S157 (≠ T166), G158 (= G167), G160 (= G169), M161 (≠ K170), N324 (= N321), L325 (= L322)
4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/359 of 4r5hA
- binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ G78), T94 (≠ S99), S95 (= S100), R98 (= R103), N126 (= N135), C127 (= C136), Q154 (= Q163), G158 (= G167), K222 (= K219), R244 (= R241), H251 (= H248)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), T10 (= T15), G11 (= G16), A12 (≠ L17), V13 (= V18), A35 (= A40), S36 (= S41), R38 (= R43), S39 (= S44), T56 (≠ P61), S70 (= S75), A71 (= A76), G72 (= G77), T75 (≠ A80), N93 (= N98), T94 (≠ S99), P125 (= P134), N126 (= N135), C127 (= C136), G160 (= G169), M161 (≠ K170), G328 (= G325)
4r4jA Crystal structure of complex sp_asadh with 3-carboxypropyl-phthalic acid and nicotinamide adenine dinucleotide phosphate (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/359 of 4r4jA
- binding 3-(3-carboxypropyl)benzene-1,2-dicarboxylic acid: T94 (≠ S99), S95 (= S100), R98 (= R103), N126 (= N135), C127 (= C136), Q154 (= Q163), G158 (= G167), E219 (= E216), K222 (= K219), R244 (= R241), H251 (= H248)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), T10 (= T15), G11 (= G16), A12 (≠ L17), V13 (= V18), A35 (= A40), S36 (= S41), R38 (= R43), S39 (= S44), T56 (≠ P61), S70 (= S75), A71 (= A76), G72 (= G77), T75 (≠ A80), N93 (= N98), T94 (≠ S99), N126 (= N135), C127 (= C136), G160 (= G169), M161 (≠ K170), G328 (= G325)
3pyxB Crystals structure of aspartate beta-semialdehyde dehydrogenase complex with NADP and 2-aminoterephthalate (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/359 of 3pyxB
- binding 2-aminobenzene-1,4-dicarboxylic acid: R98 (= R103), G158 (= G167), E219 (= E216), K222 (= K219), R244 (= R241)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), T10 (= T15), G11 (= G16), A12 (≠ L17), V13 (= V18), A35 (= A40), S36 (= S41), R38 (= R43), S39 (= S44), T56 (≠ P61), S70 (= S75), A71 (= A76), G72 (= G77), T75 (≠ A80), C127 (= C136), S157 (≠ T166), G158 (= G167), G160 (= G169), M161 (≠ K170), N324 (= N321), L325 (= L322)
3pylC Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with d-2,3-diaminopropionate (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/361 of 3pylC
4r51A Crystal complex structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide adenine dinucleotide phosphate and phthalic acid (see paper)
49% identity, 97% coverage: 6:337/341 of query aligns to 1:340/360 of 4r51A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), T10 (= T15), G11 (= G16), A12 (≠ L17), V13 (= V18), A35 (= A40), S36 (= S41), S39 (= S44), T56 (≠ P61), S70 (= S75), A71 (= A76), G72 (= G77), N93 (= N98), T94 (≠ S99), N126 (= N135), C127 (= C136), G160 (= G169), M161 (≠ K170), G328 (= G325)
- binding phthalic acid: S73 (≠ G78), T94 (≠ S99), S95 (= S100), R98 (= R103), N126 (= N135), K222 (= K219)
8jusA Crystal structure of aspartate semialdehyde dehydrogenase from porphyromonas gingivalis complexed with 2',5'adenosine diphosphate
47% identity, 98% coverage: 8:340/341 of query aligns to 2:332/335 of 8jusA
3tz6A Crystal structure of aspartate semialdehyde dehydrogenase complexed with inhibitor smcs (cys) and phosphate from mycobacterium tuberculosis h37rv (see paper)
42% identity, 97% coverage: 6:337/341 of query aligns to 1:340/342 of 3tz6A
- active site: C129 (= C136), Q156 (= Q163), R248 (= R241), H255 (= H248)
- binding cysteine: C129 (= C136), Q156 (= Q163), G160 (= G167), E223 (= E216), R248 (= R241), H255 (= H248)
- binding glycerol: S108 (≠ P113), G187 (= G182), F192 (≠ D187), P201 (≠ Q196), Q225 (≠ M218), R228 (≠ V221), F229 (≠ N222), Q335 (= Q332), E338 (= E335), L339 (= L336)
- binding sulfate ion: R98 (= R103), H117 (≠ K126), R119 (≠ A128), N128 (= N135), C129 (= C136), K226 (= K219), E270 (≠ K263), R273 (= R266)
Query Sequence
>WP_012501543.1 NCBI__GCF_000020505.1:WP_012501543.1
MSSDAGYRVAVLGATGLVGRTMIKVLEERDFPVSELVPLASPRSRGEVVTFKGREFVTEV
PSPEIFEGVDIALFSAGGSASLEWAPVAAQAGAIVIDNSSAFRMEEGVPLVVPEVNAESI
FDREGKAAPIIANPNCSTIQMVVALKPLYDAYGISRIVVSTYQSVTGKGKAGRDALESEL
AGNIPEDPETFTHFHQIAFNAVPQIDAFTENGYTKEEMKMVNETRKIFDDQEIQVSPTTV
RIPVYGGHGESVNVELKRDYDIKMVRALLAGSPGIVMQDDPSARIYPMPMTSYERDEVFV
GRLRPDYWHPRTLNLWVVADNLRKGAATNAVQIAELLVGNL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory