SitesBLAST

A0A0M0ELU2 Bifunctional N-acyl-homoserine lactone acylase/prephenate dehydratase; Bifunctional AHL acylase/PDT; Quorum quenching protein GqqA; EC 3.5.1.97; EC 4.2.1.51 from Komagataeibacter europaeus (Gluconacetobacter europaeus) (see paper)
38% identity, 99% coverage: 4:280/281 of query aligns to 5:276/281 of A0A0M0ELU2

query
sites
A0A0M0ELU2

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R25 (≠ E25) mutation to S: Affects dimerization. Loss of N-acyl-homoserine lactone acylase activity.
T118 (= T115) mutation to S: Does not affect N-acyl-homoserine lactone acylase activity.
PPPGP 187:191 (≠ QPDMA 191:195) mutation to AS: Loss of N-acyl-homoserine lactone acylase activity.
A207 (≠ S211) binding L-phenylalanine
L208 (= L212) binding L-phenylalanine
N221 (≠ D225) binding L-phenylalanine
M222 (≠ L226) binding L-phenylalanine
F261 (≠ E265) mutation to S: Loss of N-acyl-homoserine lactone acylase activity.

Sites not aligning to the query:

279:281 mutation Missing: Does not affect N-acyl-homoserine lactone acylase activity.

7am0B Gqqa- a novel type of quorum quenching acylases (see paper)
38% identity, 99% coverage: 4:280/281 of query aligns to 2:273/278 of 7am0B

query
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7am0B

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binding phenylalanine: N199 (≠ H206), N200 (= N207), Q201 (≠ E208), P202 (≠ Q209), L205 (= L212), N218 (≠ D225), M219 (≠ L226), T220 (= T227), R221 (≠ K228), T234 (≠ Y241), F236 (= F243)

6vh5D Crystal structure of prephenate dehydratase from brucella melitensis biovar abortus 2308 in complex with phenylalanine
38% identity, 98% coverage: 2:276/281 of query aligns to 6:276/282 of 6vh5D

query
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6vh5D

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binding phenylalanine: N207 (= N207), V208 (≠ E208), L212 (= L212), N225 (≠ D225), M226 (≠ L226), T227 (= T227), K228 (= K228), S231 (= S231), T241 (≠ Y241), F243 (= F243)

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
33% identity, 98% coverage: 6:281/281 of query aligns to 106:383/386 of P0A9J8

query
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P0A9J8

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Sites not aligning to the query:

11 mutation R->A,K: Important decrease in catalytic efficiency and affinity.
28 mutation R->A,K: Important decrease in catalytic efficiency and affinity.
39 mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
52 mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
88 mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

3mwbA The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
30% identity, 96% coverage: 8:276/281 of query aligns to 5:274/306 of 3mwbA

query
sites
3mwbA

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I

A

R

E

E

T

A

F

L

V

V

K

P

V

I

E

T

H

F

C

V

L

L

L

V

G

A

L

R

P

P

G

G

S

V

S

E

V

L

E

S

R

D

A

I

T

K

K

R

A

I

M

S

S

T

H

H

P

G

Q

H

A

A

L

W

A

A

Q

Q

C

C

H

R

N

L

F

W

F

V

A

D

T

E

H

H

-

L

P

P

N

N

V

A

K

D

A

Y

E

V

A

P

T

G

Y

S

D

S

T

T

A

A

G

A

S

S

A

A

K

M

M

G

V

L

A

L

E

E

Q

D

G

D

D

A

P

P

T

Y

A

E

L

A

A

A

I

I

A

C

S

A

K

P

R

L

-

I

A

A

G

A

E

E

L

Q

Y

P

G

G

L

L

E

N

I

V

L

L

K

A

E

E

N

D

L

I

A

G

D

D

E

N

E

P

W

D

N

A

I

V

T
|
T

R

R

F
|
F

F

I

C

L

I

V

A

S

R

R

E

-

D

-

N

-

E

-

A

-

G

-

L

-

S

P

G

G

L

A

L

L

N

P

Q

E

P

R

D

T

M

G

A

A

N

D

P

K

K

T

T

T

S

V

I

V

V

V

F

P

S

L

L

P

H

E

N
x
D

E
x
H

Q

P

G

G

S

A

L
|
L

Y

M

K

E

A

I

L

L

A

D

T

Q

L

F

A

A

H

S

R

R

G

G

I

V

D
x
N

L
|
L

T
x
S

K
x
R

I

I

E

E

S
|
S

R

R

P

P

S

T

R

G

K

Q

K

Y

A

L

F

G

E

H

Y

Y

L

F

F
|
F

Y

S

A

I

D

D

F

A

I

D

G

G

H

H

H

A

D

T

D

D

P

S

L

R

I

V

Q

A

R

D

A

A

L

L

D

A

N

G

L

L

R

H

E

R

F

I

A

S

P

P

M

A

L

T

K

R

V

F

L

L

G

G

S

S

Y

Y

active site: T176 (= T171), F178 (= F173)
binding phenylalanine: D205 (≠ N207), H206 (≠ E208), L210 (= L212), N223 (≠ D225), L224 (= L226), S225 (≠ T227), R226 (≠ K228), S229 (= S231), F241 (= F243)

3mwbB The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
30% identity, 96% coverage: 8:276/281 of query aligns to 5:271/303 of 3mwbB

query
sites
3mwbB

Y

F

Q

L

G

G

E

P

P

Q

G

G

A

T

Y

F

S

T

E

E

I

A

A

A

A

L

L

M

R

Q

F

V

-

P

-

G

-

A

-

D

G

A

E

T

P

R

K

I

P

P

C

C

E

T

S

N

F

V

D

N

D

T

V

A

F

L

T

E

A

R

V

V

T

R

D

A

G

G

E

E

A

A

D

D

Y

A

A

A

A

M

I

V

P

P

I

I

E

E

N

N

S

S

L

V

G

E

G

G

S

G

I

V

H

T

Q

A

N

T

Y

L

D

D

L

A

L

I

L

A

R

T

-

G

R

Q

P

E

V

L

V

R

I

I

L

I

A

R

E

E

T

A

F

L

V

V

K

P

V

I

E

T

H

F

C

V

L

L

L

V

G

A

L

R

P

P

G

G

S

V

S

E

V

L

E

S

R

D

A

I

T

K

K

R

A

I

M

S

S

T

H

H

P

G

Q

H

A

A

L

W

A

A

Q

Q

C

C

H

R

N

L

F

W

F

V

A

D

T

E

H

H

-

L

P

P

N

N

V

A

K

D

A

Y

E

V

A

P

T

G

Y

S

D

S

T

T

A

A

G

A

S

S

A

A

K

M

M

G

V

L

A

L

E

E

Q

D

G

D

D

A

P

P

T

Y

A

E

L

A

A

A

I

I

A

C

S

A

K

P

R

L

-

I

A
|
A

G

A

E

E

L
x
Q

Y

P

G

G

L
|
L

E

N

I

V

L

L

K

A

E

E

N

D

L

I

A

G

D

D

E

N

E

P

W

D

N

A

I

V

T
|
T

R

R

F
|
F

F

I

C

L

I

V

A

S

R

R

E

-

D

-

N

-

E

-

A

-

G

-

L

-

S

P

G

G

L

A

L

L

N

P

Q

E

P

R

D

T

M

G

A

A

N

D

P

K

K

T

T

T

S

V

I

V

V

V

F

P

S

L

L

P

H

E

N
x
D

E
x
H

Q

P

G

G

S

A

L
|
L

Y

M

K

E

A

I

L

L

A

D

T

Q

L

F

A

A

H

S

R

R

G

G

I

V

D
x
N

L
|
L

T
x
S

K
x
R

I
|
I

E

E

S

S

R

R

P

P

S

T

R

L

K

G

K

-

A

-

F

-

E

H

Y
|
Y

L

F

F
|
F

Y

S

A

I

D

D

F

A

I

D

G

G

H

H

H

A

D

T

D

D

P

S

L

R

I

V

Q

A

R

D

A

A

L

L

D

A

N

G

L

L

R

H

E

R

F

I

A

S

P

P

M

A

L

T

K

R

V

F

L

L

G

G

S

S

Y

Y

active site: T176 (= T171), F178 (= F173)
binding magnesium ion: A155 (= A150), Q158 (≠ L153), L161 (= L156)
binding phenylalanine: D205 (≠ N207), H206 (≠ E208), L210 (= L212), N223 (≠ D225), L224 (= L226), S225 (≠ T227), R226 (≠ K228), I227 (= I229), Y236 (= Y241), F238 (= F243)

3luyA Putative chorismate mutase from bifidobacterium adolescentis
25% identity, 98% coverage: 1:276/281 of query aligns to 1:283/326 of 3luyA

query
sites
3luyA

M

M

T

S

N

A

R

R

L

K

I

L

A

F

Y

Y

Q

L

G

G

E

P

P

Q

G

G

A

T

Y

F

S

T

E

H

I

Q

A

A

L

V

-

N

-

A

-

Q

-

E

-

L

-

A

R

R

F

F

G

-

E

E

P

P

K

Q

-

G

-

F

-

D

-

L

-

M

P

P

C

M

E

D

S

D

F

V

D

P

D

Q

V

I

F

L

T

D

A

A

V

A

T

Q

D

H

G

G

E

D

A

G

D

-

Y

W

A

G

A

I

I

V

P

A

I

W

E

E

N

N

S

N

L

V

G

E

G

G

S

Y

I

V

H

V

Q

P

N

N

Y

L

D

D

L

A

L

L

L

I

R

D

-

A

R

K

P

D

V

L

V

V

I

G

L

F

A

A

E

R

T

V

F

G

V

V

K

N

V

V

E

E

H

F

C

D

L

A

L

Y

G

V

L

A

P

Q

G

G

S

A

S

D

V

P

E

A

R

E

A

A

T

R

K

I

A

A

M

T

S

A

H

H

P

P

Q

H

A

G

L

L

A

A

Q

Q

C

C

H

K

N

R

F

F

F

I

A

A

T

E

H

H

P

R

N

L

V

S

K

T

A

Q

E

P

A

A

T

T

Y

S

D

N

T

A

A

A

G

A

S

C

A

R

K

D

M

L

V

I

A

-

E

-

Q

-

G

-

D

-

P

P

T

G

A

E

L

I

A

A

I

F

A

G

S

P

K

A

R

I

A

C

G

G

E

E

L

L

Y

Y

G

D

L

I

E

T

I

R

L

I

K

G

E

T

N

A

L

I

A

Q

D

D

E

Y

E

Q

W

G

N

A

I

A

T
|
T

R

D

F

F

F

L

C

V

I

L

A

S

R

-

E

-

D

-

N

P

E

R

A

A

G

E

L

V

S

A

G

R

L

L

N

A

Q

K

P

P

D

R

M

A

-

E

A

A

N

N

P

V

K

E

T

Y

S

E

I

S

V

V

F

L

S

T

-

L

-

I

-

P

L

L

H

V

N

T

E
x
G

Q

P

G

G

S

V

L
|
L

Y

A

K

N

A

L

L

L

A

D

T

V

L

F

A

R

H

D

R

A

G

G

I

L

D

N

L

M

T

T

K

S

I

F

E

I

S

S

R

R

P

P

S

I

R

K

K

G

K

R

A

T

F

G

E

T

Y
|
Y

L

S

F
|
F

Y

I

A

V

D

T

F

L

I

D

G

A

H

A

H

P

D

W

D

E

P

E

L

R

I

F

Q

R

R

D

A

A

L

L

D

V

N

E

L

I

R

A

E

E

F

H

A

G

P

D

M

W

L

A

K

K

V

T

L

L

G

A

S

V

Y

Y

active site: T177 (= T171)
binding 3-phenylpyruvic acid: G215 (≠ E208), L219 (= L212), Y248 (= Y241), F250 (= F243)

7alzA Gqqa- a novel type of quorum quenching acylases (see paper)
32% identity, 45% coverage: 155:280/281 of query aligns to 72:189/194 of 7alzA

query
sites
7alzA

G

G

L

L

E

F

I

I

L

V

K

G

E

E

N

H

L

F

A

-

D

Q

E

R

E

V

W

H

N

N

I

T

T

T

R

R

F

F

F

Y

C

I

I

A

A

S

R

R

E

R

D

P

N

A

E

T

A

L

G

-

L

-

S

-

G

-

L

-

N

-

Q

P

P

P

D

P

M

G

A

P

N

G

P

F

K

M

T

T

S

T

I

L

V

L

F

F

S

R

L

V

H

N

N

N

E
x
Q

Q

P

G

G

S

A

L
|
L

Y

Y

K

K

A

A

L

L

A

G

T

G

L

L

A

A

H

T

R

A

G

G

I

V

D
x
N

L
x
M

T
|
T

K
x
R

I

L

E

E

S

S

R

Y

P

M

S

L

R

E

K

G

K

S

A

F

F

S

E

A

Y
x
T

L

Q

F
|
F

Y

L

A

M

D

D

F

V

I

E

G

G

H

H

H

P

D

E

D

A

P

P

L

P

I

L

Q

A

R

R

A

A

L

L

D

D

N

E

L

L

R

S

E

F

F

F

A

S

P

E

M

Q

L

Q

K

E

V

I

L

L

G

G

S

V

Y

Y

G

P

V

A

V

S

P

P

binding phenylalanine: Q117 (≠ E208), L121 (= L212), N134 (≠ D225), M135 (≠ L226), T136 (= T227), R137 (≠ K228), T150 (≠ Y241), F152 (= F243)

P16331 Phenylalanine-4-hydroxylase; PAH; Phe-4-monooxygenase; EC 1.14.16.1 from Mus musculus (Mouse) (see paper)
38% identity, 25% coverage: 196:264/281 of query aligns to 32:99/453 of P16331

query
sites
P16331

N

N

P

G

K

A

T

V

S

S

I

L

V

I

F

F

S

S

L

L

H

K

N

E

E

E

Q

V

G

G

S

A

L

L

Y

A

K

K

A

V

L

L

A

R

T

L

L

F

A

E

H

E

R

N

G

E

I

I

D

N

L

L

T

T

K

H

I

I

E

E

S

S

R

R

P

P

S

S

R

R

K

L

K

N

A

K

F

D

E

E

Y

Y

L

E

F

F

Y

F

A

T

D

-

F

Y

I

L

G

D

H

K

H

R

D

S

D

K

P

P

L

V

I

L

Q

G

R

S

A

I

L

I

D

K

N

S

L

L

R

R

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
106 V→A: Mutant mice have mild features of phenylketonuria.
263 F→S: Mutant mice have features of phenylketonuria.

P04176 Phenylalanine-4-hydroxylase; PAH; Phe-4-monooxygenase; EC 1.14.16.1 from Rattus norvegicus (Rat) (see 2 papers)
38% identity, 25% coverage: 196:264/281 of query aligns to 32:99/453 of P04176

query
sites
P04176

N

N

P

G

K

A

T

I

S

S

I

L

V

I

F

F

S

S

L

L

H

K

N

E

E

E

Q

V

G

G

S

A

L

L

Y

A

K

K

A

V

L

L

A

R

T

L

L

F

A

E

H

E

R

N

G

D

I

I

D

N

L

L

T

T

K

H

I

I

E

E

S

S

R

R

P

P

S

S

R

R

K

L

K

N

A

K

F

D

E

E

Y

Y

L

E

F

F

Y

F

A

T

D

-

F

Y

I

L

G

D

H

K

H

R

D

T

D

K

P

P

L

V

I

L

Q

G

R

S

A

I

L

I

D

K

N

S

L

L

R

R

Sites not aligning to the query:

16 modified: Phosphoserine; by PKA
285 binding Fe cation
290 binding Fe cation
330 binding Fe cation

5fgjA Structure of tetrameric rat phenylalanine hydroxylase, residues 1-453 (see paper)
38% identity, 25% coverage: 196:264/281 of query aligns to 13:80/428 of 5fgjA

query
sites
5fgjA

N

N

P

G

K

A

T

I

S

S

I

L

V

I

F

F

S

S

L

L

H

K

N

E

E

E

Q

V

G

G

S

A

L

L

Y

A

K

K

A

V

L

L

A

R

T

L

L

F

A

E

H

E

R

N

G

D

I

I

D

N

L

L

T

T

K

H

I

I

E

E

S

S

R

R

P

P

S

S

R

R

K

L

K

N

A

K

F

D

E

E

Y

Y

L

E

F

F

Y

F

A

T

D

-

F

Y

I

L

G

D

H

K

H

R

D

T

D

K

P

P

L

V

I

L

Q

G

R

S

A

I

L

I

D

K

N

S

L

L

R

R

Sites not aligning to the query:

active site: 263, 268, 308, 327
binding fe (iii) ion: 263, 268, 308

P70080 Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; EC 1.14.16.4 from Gallus gallus (Chicken) (see paper)
41% identity, 17% coverage: 198:246/281 of query aligns to 17:65/445 of P70080

query
sites
P70080

K

R

T

T

S

A

I

I

V

I

F

F

S

S

L

L

H

K

N

N

E

E

Q

V

G

G

S

G

L

L

Y

V

K

K

A

A

L

L

A

K

T

L

L

F

A

Q

H

E

R

K

G

H

I

V

D

N

L

L

T

V

K

H

I

I

E

E

S

S

R

R

P

K

S

S

R

K

K

R

A

N

F

S

E

E

Y

F

L

E

F

I

Y

F

A

V

D

D

Sites not aligning to the query:

236 binding L-tryptophan
258 binding L-tryptophan
266 binding L-tryptophan
273 binding Fe cation
278 binding Fe cation
318 binding Fe cation
337 binding L-tryptophan
367 binding L-tryptophan

P00439 Phenylalanine-4-hydroxylase; PAH; Phe-4-monooxygenase; EC 1.14.16.1 from Homo sapiens (Human) (see 48 papers)
46% identity, 18% coverage: 196:245/281 of query aligns to 32:81/452 of P00439

query
sites
P00439

N

N

P

G

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S

S

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F

S
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S

L
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L

H
x
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N

E

E

E

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x
V

G
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G

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x
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L
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L

Y

A

K

K

A

V

L

L

A
x
R

T

L

L
x
F

A
x
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H

E

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G

D

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D
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L

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T

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E

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R

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S

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R

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F39 (= F203) to L: in PAH deficiency; haplotype 1; dbSNP:rs62642926; natural variant: Missing (in PAH deficiency; severe; haplotypes 9,21)
S40 (= S204) to L: in PAH deficiency; severe; dbSNP:rs62642938
L41 (= L205) to F: in PAH deficiency; severe; dbSNP:rs62642928; to P: in PAH deficiency; mild; dbSNP:rs62642916
K42 (≠ H206) to I: in PAH deficiency; severe; haplotype 21; dbSNP:rs62635346
V45 (≠ Q209) to A: in PAH deficiency; severe; dbSNP:rs1592988883
G46 (= G210) to S: in PAH deficiency; severe; haplotype 5; significantly reduces phenylalanine binding; dbSNP:rs74603784
A47 (≠ S211) to V: in PAH deficiency; haplotype 4; significantly reduces phenylalanine binding; dbSNP:rs118203925
L48 (= L212) to S: in PAH deficiency; mild; haplotypes 3,4; dbSNP:rs5030841
R53 (≠ A217) to H: in PAH deficiency; severe; dbSNP:rs118092776
F55 (≠ L219) to L: in PAH deficiency; does not affect oligomerization; results in loss of substrate activation; dbSNP:rs199475598
E56 (≠ A220) to D: in PAH deficiency; severe; haplotype 10; dbSNP:rs199475567
N61 (≠ D225) to D: in PAH deficiency; severe; dbSNP:rs199475651
L62 (= L226) to P: in PAH deficiency; severe; dbSNP:rs1877437661
TH 63:64 (≠ TK 227:228) natural variant: TH -> PN (in PAH deficiency; severe; haplotype 1; abolishes phenylalanine binding)
I65 (= I229) to N: in PAH deficiency; severe; dbSNP:rs75193786; to S: in PAH deficiency; severe; results in disturbed oligomerization; results in loss of substrate activation; dbSNP:rs75193786; to T: in PAH deficiency; severe; haplotypes 1,5,9,21,B; abolishes phenylalanine binding; dbSNP:rs75193786; to V: in PAH deficiency; dbSNP:rs199475643
S67 (= S231) to P: in PAH deficiency; severe; haplotype 4; dbSNP:rs5030842
R68 (= R232) to S: in PAH deficiency; severe; haplotype 1; dbSNP:rs76394784
E76 (= E240) to A: in PAH deficiency; severe; dbSNP:rs62507347; to G: in PAH deficiency; dbSNP:rs62507347

Sites not aligning to the query:

16 modified: Phosphoserine; by PKA; S → P: in PAH deficiency; severe; uncertain significance; dbSNP:rs62642946
20 Q → L: in PAH deficiency; mild; dbSNP:rs199475662
84 D → Y: in PAH deficiency; severe; haplotype 4; dbSNP:rs62514902
87 S → R: in PAH deficiency; haplotype 1; dbSNP:rs62516151
92 T → I: in PAH deficiency; severe; dbSNP:rs62514903
94 natural variant: Missing (in PAH deficiency; mild; haplotype 2)
98 L → S: in PAH deficiency; dbSNP:rs62517167
104 A → D: in PAH deficiency; mild; haplotype 1; dbSNP:rs62642929
110 S → C: in PAH deficiency; mild
121 F → L: in PAH deficiency; mild
124 T → I: in PAH deficiency; severe; haplotype 28; dbSNP:rs199475571
129 D → Y: in PAH deficiency; severe; dbSNP:rs199475606
143 D → G: in PAH deficiency; severe; haplotype 11; dbSNP:rs199475572
145 D → V: in PAH deficiency; severe; dbSNP:rs140175796
146 H → Y: in PAH deficiency; severe; dbSNP:rs199475599
148 G → S: in PAH deficiency; severe; haplotypes 1,2,7; dbSNP:rs80297647
151 D → H: in PAH deficiency; severe; haplotypes 1,8; dbSNP:rs199475597
154 Y → N: in PAH deficiency; severe; dbSNP:rs199475587
155 R → P: in PAH deficiency; severe; dbSNP:rs199475663
157 R → N: in PAH deficiency; severe; 5% activity; requires 2 nucleotide substitutions; dbSNP:rs1565853495; R → S: in PAH deficiency; severe; dbSNP:rs199475612
158 R → Q: in PAH deficiency; severe; haplotypes 1,2,4,7,16, 28; dbSNP:rs5030843; R → W: in PAH deficiency; severe; dbSNP:rs75166491
160 Q → P: in PAH deficiency; severe; dbSNP:rs199475601
161 F → S: in PAH deficiency; severe; haplotype 4; dbSNP:rs79635844
164 I → T: in PAH deficiency; severe; haplotype 1; dbSNP:rs199475595
167 N → I: in PAH deficiency; severe; dbSNP:rs77554925; N → S: in PAH deficiency; mild; dbSNP:rs77554925
169 R → H: in PAH deficiency; severe; dbSNP:rs199475679
170 H → D: in PAH deficiency; mild; dbSNP:rs199475655; H → Q: in PAH deficiency; severe; does not affect oligomerization; dbSNP:rs199475652; H → R: in PAH deficiency; severe; dbSNP:rs199475573
171 G → A: in PAH deficiency; severe; haplotype 1; dbSNP:rs199475596; G → R: in PAH deficiency; severe; dbSNP:rs199475613
173 P → T: in PAH deficiency; severe; haplotype 4; dbSNP:rs199475574
174 I → T: in PAH deficiency; severe; haplotype 1; dbSNP:rs138809906; I → V: in PAH deficiency; severe; dbSNP:rs199475632
175 P → A: in PAH deficiency; severe; dbSNP:rs199475604
176 R → L: in PAH deficiency; dbSNP:rs74486803; R → P: in PAH deficiency; severe; dbSNP:rs74486803
177 V → L: in PAH deficiency; severe; haplotype 6; dbSNP:rs199475602; V → M: in PAH deficiency; mild; dbSNP:rs199475602
178 E → G: in PAH deficiency; dbSNP:rs77958223
183 E → Q: in PAH deficiency; severe; dbSNP:rs199475664
190 V → A: in PAH deficiency; severe; haplotype 3; dbSNP:rs62514919
194 L → P: in PAH deficiency; severe; dbSNP:rs5030844
196 S → Y: in PAH deficiency; mild; dbSNP:rs865899394
201 H → R: in PAH deficiency; severe; dbSNP:rs62517180; H → Y: in PAH deficiency; haplotype 1; dbSNP:rs62517205
204 Y → C: in PAH deficiency; mild; haplotypes 3,4; dbSNP:rs62514927
205 E → A: in PAH deficiency; severe; dbSNP:rs62508593
206 Y → D: in PAH deficiency; severe; dbSNP:rs62517170
207 N → D: in PAH deficiency; severe; dbSNP:rs62508572; N → S: in PAH deficiency; severe; haplotype 4; dbSNP:rs62508721
211 P → T: in PAH deficiency; severe; haplotype 4; dbSNP:rs62514931
212 L → P: in PAH deficiency; severe; dbSNP:rs62517198
213 L → P: in PAH deficiency; severe; dbSNP:rs62516109
217 C → G: in PAH deficiency; severe; dbSNP:rs62508718
218 G → V: in PAH deficiency; severe; haplotypes 1,2; dbSNP:rs62514933
221 E → G: in PAH deficiency; severe; haplotype 4; dbSNP:rs62514934
222 D → V: in PAH deficiency; severe; haplotypes 3,4; dbSNP:rs62507319
224 I → M: in PAH deficiency; severe; haplotype 4; dbSNP:rs199475576
225 P → R: in PAH deficiency; severe; dbSNP:rs62517204; P → T: in PAH deficiency; severe; haplotype 1; dbSNP:rs199475589
226 Q → H: in PAH deficiency; severe; dbSNP:rs62508615
230 V → I: in PAH deficiency; haplotype 4; dbSNP:rs62516152
231 S → F: in PAH deficiency; severe; dbSNP:rs62508577; S → P: in PAH deficiency; severe; dbSNP:rs5030845
233 F → L: in PAH deficiency; severe; haplotypes 2,3; dbSNP:rs62517208
238 T → P: in PAH deficiency; severe; haplotype 4; dbSNP:rs199475577
239 G → S: in PAH deficiency; severe; dbSNP:rs62517178
240 F → S: in PAH deficiency; severe; dbSNP:rs62508594
241 R → C: in PAH deficiency; haplotype 34; dbSNP:rs76687508; R → H: in PAH deficiency; severe; haplotypes 1,5; dbSNP:rs62508730; R → L: in PAH deficiency; severe; dbSNP:rs62508730
242 L → F: in PAH deficiency; severe; dbSNP:rs199475578
243 R → Q: in PAH deficiency; haplotypes 4,7,9; dbSNP:rs62508588
244 P → L: in PAH deficiency; severe; haplotype 12; dbSNP:rs118203923
245 V → A: in PAH deficiency; haplotypes 3,7; dbSNP:rs76212747; V → E: in PAH deficiency; severe; haplotype 11; dbSNP:rs76212747; V → L: in PAH deficiency; severe; dbSNP:rs62508694
246 A → D: in PAH deficiency; severe; dbSNP:rs199475610
247 G → V: in PAH deficiency; severe; haplotype 4; dbSNP:rs199475579
248 L → P: in PAH deficiency; severe; dbSNP:rs62507340
249 L → F: in PAH deficiency; severe; haplotype 1; dbSNP:rs74503222
252 R → G: in PAH deficiency; severe; haplotype 7; dbSNP:rs5030847; R → Q: in PAH deficiency; severe; haplotype 1; dbSNP:rs62644503; R → W: in PAH deficiency; severe; haplotypes 1,6,7,8,42, 69; complete loss of activity; dbSNP:rs5030847
255 L → S: in PAH deficiency; severe; haplotype 36; dbSNP:rs62642930; L → V: in PAH deficiency; severe; haplotypes 18,21; dbSNP:rs62642931
257 G → C: in PAH deficiency; severe; dbSNP:rs5030848
259 A → T: in PAH deficiency; severe; haplotype 3; dbSNP:rs62642932; A → V: in PAH deficiency; severe; haplotypes 7,42; dbSNP:rs118203921
261 R → P: in PAH deficiency; severe; dbSNP:rs5030849; R → Q: in PAH deficiency; haplotypes 1,2,4,22, 24,28; dbSNP:rs5030849
263 F → L: in PAH deficiency; severe; dbSNP:rs62642944
264 H → L: in PAH deficiency; severe; dbSNP:rs199475580
265 C → G: in PAH deficiency; severe; dbSNP:rs62517181
269 I → L: in PAH deficiency; dbSNP:rs62508692
270 R → K: in PAH deficiency; severe; dbSNP:rs62514950; R → S: in PAH deficiency; severe; haplotype 1; dbSNP:rs62514951
271 H → Y: in PAH deficiency; severe; dbSNP:rs62517164
273 S → F: in PAH deficiency; severe; haplotype 7; dbSNP:rs62514953
274 K → E: in PAH deficiency; severe; dbSNP:rs142934616
275 P → L: in PAH deficiency; severe; reduced activity; increased affinity for the substrate; mildly reduced substrate activation; decreased cofactor affinity; dbSNP:rs62508715
276 M → I: in PAH deficiency; severe; dbSNP:rs62514954; M → V: in PAH deficiency; severe; haplotype 4; dbSNP:rs62516149
277 Y → C: in PAH deficiency; severe; dbSNP:rs62516155; Y → D: in PAH deficiency; severe; haplotype 2; dbSNP:rs78655458
278 T → A: in PAH deficiency; severe; dbSNP:rs62516156; T → N: in PAH deficiency; severe; dbSNP:rs62507262
280 E → K: in PAH deficiency; severe; haplotypes 1,2,4,16,38; partial residual activity; dbSNP:rs62508698
281 P → L: in PAH deficiency; severe; haplotypes 1,4; dbSNP:rs5030851
282 D → N: in PAH deficiency; severe; haplotype 1; dbSNP:rs199475582
283 I → F: in PAH deficiency; severe; haplotype 21; dbSNP:rs62517168; I → N: in PAH deficiency; severe; dbSNP:rs62508693; I→C: Loss of positive cooperativity and reduction of fold-activation by L-Phe preincubation.
290 H → Y: in PAH deficiency; severe; dbSNP:rs1486763160
297 R → C: in PAH deficiency; severe; haplotype 4; dbSNP:rs62642945; R → H: in PAH deficiency; severe; dbSNP:rs62642939
299 F → C: in PAH deficiency; severe; haplotype 8; dbSNP:rs62642933
300 A → S: in PAH deficiency; haplotype 1; does not affect oligomerization; reduction in activity is probably due to a global conformational change in the protein that reduces allostery; dbSNP:rs5030853; A → V: in PAH deficiency; severe; dbSNP:rs199475609
303 S → P: in PAH deficiency; severe; haplotype 5; dbSNP:rs199475608
304 Q → R: in PAH deficiency; severe; dbSNP:rs199475592
306 I → V: in PAH deficiency; haplotype 4; dbSNP:rs62642934
309 A → D: in PAH deficiency; severe; haplotype 7; dbSNP:rs62642935; A → V: in PAH deficiency; severe; dbSNP:rs62642935
310 S → F: in PAH deficiency; severe; haplotype 7; dbSNP:rs62642913; S → Y: in PAH deficiency; mild; reduction in activity is probably due to a global conformational change in the protein that reduces allostery; dbSNP:rs62642913
311 L → P: in PAH deficiency; severe; haplotypes 1,7,10; dbSNP:rs62642936
314 P → H: in PAH deficiency; severe; dbSNP:rs62642940; P → S: in PAH deficiency; mild; does not affect oligomerization; reduction in activity is probably due to a global conformational change in the protein that reduces allostery; dbSNP:rs199475650
318 I → T: in PAH deficiency; severe; partial loss of activity; dbSNP:rs62642918
322 A → G: in PAH deficiency; severe; haplotype 12; dbSNP:rs62514958; A → T: in PAH deficiency; severe; haplotype 1; dbSNP:rs62514957; A → V: in PAH deficiency; severe; dbSNP:rs62514958
325 Y → C: in PAH deficiency; severe; dbSNP:rs62508578
330 E → D: in PAH deficiency; severe; dbSNP:rs62508580
331 F → L: in PAH deficiency; severe; haplotype 1; dbSNP:rs62517179
333 L → F: in PAH deficiency; severe; dbSNP:rs62516060
334 C → S: in PAH deficiency; severe; dbSNP:rs62517174
337 G → V: in PAH deficiency; severe; dbSNP:rs62517206
338 D → Y: in PAH deficiency; severe; haplotype 4; dbSNP:rs62516150
341 K → R: in PAH deficiency; severe; dbSNP:rs62516153; K → T: in PAH deficiency; severe; dbSNP:rs62516153
342 A → T: in PAH deficiency; severe; haplotype 5; dbSNP:rs62507282
343 Y → C: in PAH deficiency; severe; dbSNP:rs62507265
344 G → R: in PAH deficiency; severe; dbSNP:rs62508679; G → V: in PAH deficiency; severe; dbSNP:rs62508582
345 A → S: in PAH deficiency; severe; dbSNP:rs62516062; A → T: in PAH deficiency; severe; haplotype 7; dbSNP:rs62516062
347 L → F: in PAH deficiency; severe; dbSNP:rs62516154
348 L → V: in PAH deficiency; mild; haplotype 9; dbSNP:rs62516092
349 S → L: in PAH deficiency; severe; dbSNP:rs62507279; S → P: in PAH deficiency; severe; haplotypes 1,4; dbSNP:rs62508646
350 S → T: in PAH deficiency; severe; haplotype 2; dbSNP:rs62517183
357 C → G: in PAH deficiency; severe; dbSNP:rs62508595
362 P → T: in PAH deficiency; severe; dbSNP:rs62507329
364 natural variant: Missing (in PAH deficiency; severe; haplotype 5; dbSNP:rs62516096)
364:368 natural variant: Missing (in PAH deficiency; severe; dbSNP:rs62516096)
366 P → H: in PAH deficiency; severe; dbSNP:rs62516098
372 T → S: in PAH deficiency; severe; dbSNP:rs62517163
377 Y → C: in PAH deficiency; severe; haplotype 4; dbSNP:rs62642942
380 T → M: in PAH deficiency; haplotype 4; dbSNP:rs62642937
386 Y → C: in PAH deficiency; severe; dbSNP:rs62516141
387 Y → H: in PAH deficiency; severe; haplotype 1; dbSNP:rs62517194
388 V → L: in PAH deficiency; severe; dbSNP:rs62516101; V → M: in PAH deficiency; severe; haplotypes 1,4; dbSNP:rs62516101
390 E → G: in PAH deficiency; haplotype 4; dbSNP:rs5030856
394 D → A: in PAH deficiency; severe; dbSNP:rs62516102; D → H: in PAH deficiency; severe; dbSNP:rs62516142
395 A → G: in PAH deficiency; severe; dbSNP:rs62508736; A → P: in PAH deficiency; severe; haplotype 1; dbSNP:rs62516103
403 A → V: in PAH deficiency; haplotype 43; dbSNP:rs5030857
407 P → L: in PAH deficiency; severe; dbSNP:rs62644473; P → S: in PAH deficiency; severe; dbSNP:rs62644465
408 R → Q: in PAH deficiency; severe; haplotypes 4,12; dbSNP:rs5030859; R → W: in PAH deficiency; haplotypes 1,2,4,5,13,34,41,44; most common mutation; reduction in activity is probably due to a global conformational change in the protein that reduces allostery; dbSNP:rs5030858
410 F → S: in PAH deficiency; mild; dbSNP:rs62644475
413 R → P: in PAH deficiency; haplotype 4; dbSNP:rs79931499; R → S: in PAH deficiency; severe; haplotype 1; dbSNP:rs62644467
414 Y → C: in PAH deficiency; haplotype 4; does not affect oligomerization; reduction in activity is probably due to a global conformational change in the protein that reduces allostery; dbSNP:rs5030860
415 D → N: in PAH deficiency; haplotype 1; dbSNP:rs62644499
417 Y → H: in PAH deficiency; severe; reduction in activity is probably due to a global conformational change in the protein that reduces allostery; dbSNP:rs62644471
418 T → P: in PAH deficiency; severe; haplotype 4; dbSNP:rs62644501
421 I → S: in PAH deficiency; severe; dbSNP:rs199475696
430 L → P: in PAH deficiency; severe; dbSNP:rs199475607
447 A → D: in PAH deficiency; severe; dbSNP:rs76542238

5fiiB Structure of a human aspartate kinase, chorismate mutase and tyra domain. (see paper)
48% identity, 16% coverage: 200:245/281 of query aligns to 3:48/78 of 5fiiB

query
sites
5fiiB

S

S

I

L

V

I

F

F

S

S

L

L

H

K

N
x
E

E
|
E

Q

V

G

G

S

A

L
|
L

Y

A

K

K

A

V

L

L

A

R

T

L

L

F

A

E

H

E

R

N

G

D

I

V

D
x
N

L
|
L

T

T

K
x
H

I
|
I

E

E

S
|
S

R

R

P

P

S

S

R

R

K

L

K

K

A

K

F

D

E

E

Y
|
Y

L

E

F
|
F

Y

F

A

T

binding phenylalanine: E10 (≠ N207), E11 (= E208), L15 (= L212), N28 (≠ D225), L29 (= L226), H31 (≠ K228), I32 (= I229), S34 (= S231), Y44 (= Y241), F46 (= F243)

Query Sequence

>WP_012501779.1 NCBI__GCF_000020505.1:WP_012501779.1
MTNRLIAYQGEPGAYSEIAALRFGEPKPCESFDDVFTAVTDGEADYAAIPIENSLGGSIH
QNYDLLLRRPVVILAETFVKVEHCLLGLPGSSVERATKAMSHPQALAQCHNFFATHPNVK
AEATYDTAGSAKMVAEQGDPTALAIASKRAGELYGLEILKENLADEEWNITRFFCIARED
NEAGLSGLLNQPDMANPKTSIVFSLHNEQGSLYKALATLAHRGIDLTKIESRPSRKKAFE
YLFYADFIGHHDDPLIQRALDNLREFAPMLKVLGSYGVVPA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory