SitesBLAST

W22 (≠ I25) mutation to A: Decreased heat stability.
E26 (≠ D29) mutation to Q: Increased dissociation of dodecamers into trimers.
M30 (≠ H33) mutation to A: Increased dissociation of dodecamers into trimers.
W34 (≠ Q37) mutation to A: Increased dissociation of dodecamers into trimers.
Y228 (= Y231) mutation to C: Becomes active at low temperatures; when associated with G-278.
A241 (≠ M244) mutation to D: Becomes active at low temperatures; when associated with G-278.
E278 (= E281) mutation to G: Becomes active at low temperatures; when associated with C-228 or D-241.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8qevA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with carbamoyl phosphate (see paper)
44% identity, 90% coverage: 11:314/336 of query aligns to 2:296/297 of 8qevA

query
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8qevA

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binding phosphoric acid mono(formamide)ester: S52 (= S63), M53 (≠ L64), R54 (= R65), T55 (= T66), R103 (= R114), C255 (= C273), L256 (≠ M274), R283 (= R301)

Q81M99 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Bacillus anthracis
45% identity, 90% coverage: 11:313/336 of query aligns to 12:309/316 of Q81M99

query
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STRT 57:60 (≠ SLRT 63:66) binding carbamoyl phosphate
Q84 (≠ G90) binding carbamoyl phosphate
R108 (= R114) binding carbamoyl phosphate
HPCQ 135:138 (= HPCQ 141:144) binding carbamoyl phosphate
N166 (= N172) binding L-ornithine
D230 (= D233) binding L-ornithine
SM 234:235 (= SM 237:238) binding L-ornithine
CL 269:270 (≠ CM 273:274) binding carbamoyl phosphate
R297 (= R301) binding carbamoyl phosphate

4nf2A Crystal structure of anabolic ornithine carbamoyltransferase from bacillus anthracis in complex with carbamoyl phosphate and l- norvaline
44% identity, 90% coverage: 11:313/336 of query aligns to 8:305/307 of 4nf2A

query
sites
4nf2A

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active site: R55 (= R65), T56 (= T66), R83 (≠ E93), R104 (= R114), H131 (= H141), Q134 (= Q144), D226 (= D233), C265 (= C273), R293 (= R301)
binding phosphoric acid mono(formamide)ester: S53 (= S63), T54 (≠ L64), R55 (= R65), T56 (= T66), R104 (= R114), H131 (= H141), Q134 (= Q144), C265 (= C273), L266 (≠ M274), R293 (= R301)
binding norvaline: L126 (= L136), N162 (= N172), D226 (= D233), S230 (= S237), M231 (= M238)

7nouA Crystal structure of mycobacterium tuberculosis argf in complex with (3,5-dichlorophenyl)boronic acid.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:305/308 of 7nouA

query
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7nouA

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active site: R102 (= R114), H129 (= H141), Q132 (= Q144), D225 (= D233), C265 (= C273), R293 (= R301)
binding [3,5-bis(chloranyl)phenyl]-oxidanyl-oxidanylidene-boron: I46 (= I58), T52 (≠ L64), R53 (= R65), R53 (= R65), F56 (≠ V68), F56 (≠ V68), L79 (≠ V91), D82 (≠ R94), E83 (= E95), V91 (≠ L103), Y95 (= Y107), L266 (≠ M274), R293 (= R301)

7nosA Crystal structure of mycobacterium tuberculosis argf in complex with 4-bromo-6-(trifluoromethyl)-1h-benzo[d]imidazole.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:305/308 of 7nosA

query
sites
7nosA

R

R

P

P

I

L

R

Q

H

G

-

P

K

R

T

G

V

V

A

A

M

V

I
|
I

F

F

N

D

K

K

P

N

S

S

L

T

R

R

T

T

R

R

V

F

S

S

F

F

E

E

L

L

G

G

V

I

Y

A

E

Q

L

L

G

G

H

H

A

A

I

V

S

V

L

V

E

D

G

S

K

G

S

S

I

T

G

Q

V

L

G

G

E

R

R
x
D

E
|
E

S

T

V

L

E

Q

D

D

I

T

A

A

R

K

L
x
V

L
|
L

S

S

R

R

Y

Y

N

V

D

D

A

A

I

I

V

V

A

W

R
|
R

L

T

H

F

E

G

H

Q

E

E

I

R

I

L

E

D

T

A

L

M

A

A

A

S

H

V

A

A

S

T

I

V

P

P

V

V

I

I

N

N

A

A

L

L

T

S

D

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

L

L

A

A

D

D

A

L

F

Q

T

T

L

I

Y

A

E

E

-

R

K

K

G

G

L

A

W

L

R

R

D

G

D

-

I

L

K

R

V

L

V

S

F

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

A

A

N

H

S

S

W

L

I

L

E

-

L

L

A

G

G

G

I

V

L

T

P

A

-

G

F

I

H

H

F

V

V

T

L

V

T

A

C

A

P

P

E

E

G

G

Y

F

L

L

P

P

D

D

E

P

T

S

L

V

L

R

K

A

Q

A

A

A

R

E

A

R

N

R

A

A

K

Q

S

D

V

T

-

G

-

A

-

S

I

V

E

T

I

V

V

T

H

A

D

D

P

A

M

H

E

A

A

A

K

A

D

G

A

A

D

D

V

V

L

L

Y

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Q

Q

E

E

D

N

E

D

M

G

A

L

E

D

R

R

L

V

K

K

T

P

F

F

E

R

P

P

Y

F

Q

Q

I

L

N

N

S

S

K

R

M

L

A

A

E

L

A

A

K

D

S

S

S

D

A

A

V

I

V

V

M

L

H

H

C
|
C

M

L

P

P

A

A

H

H

R

R

G

G

Q

D

E

E

I

I

S

T

A

D

E

A

V

V

M

M

D

D

G

G

P

P

Q

A

S

S

I

A

I

V

I

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

V

V

K

W

L

L

M

L

active site: R102 (= R114), H129 (= H141), Q132 (= Q144), D225 (= D233), C265 (= C273), R293 (= R301)
binding 4-bromanyl-6-(trifluoromethyl)-1~{H}-benzimidazole: I46 (= I58), D82 (≠ R94), E83 (= E95), V91 (≠ L103), L92 (= L104)

7norA Crystal structure of mycobacterium tuberculosis argf in complex with 2-fluoro-4-hydroxybenzonitrile.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:305/308 of 7norA

query
sites
7norA

R

R

P

P

I

L

R

Q

H

G

-

P

K

R

T

G

V

V

A

A

M

V

I

I

F

F

N

D

K

K

P

N

S

S

L
x
T

R

R

T

T

R

R

V
x
F

S

S

F

F

E

E

L

L

G

G

V

I

Y

A

E

Q

L

L

G

G

H

H

A

A

I

V

S

V

L

V

E

D

G

S

K

G

S

S

I
x
T

G

Q

V
x
L

G

G

E

R

R

D

E

E

S

T

V

L

E

Q

D

D

I

T

A

A

R

K

L

V

L

L

S

S

R

R

Y

Y

N

V

D

D

A

A

I

I

V

V

A

W

R
|
R

L

T

H

F

E

G

H

Q

E

E

I

R

I

L

E

D

T

A

L

M

A

A

A

S

H

V

A

A

S

T

I

V

P

P

V

V

I

I

N

N

A

A

L

L

T

S

D

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

L

L

A

A

D

D

A

L

F

Q

T

T

L

I

Y

A

E

E

-

R

K

K

G

G

L

A

W

L

R

R

D

G

D

-

I

L

K

R

V

L

V

S

F

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

A

A

N

H

S

S

W

L

I

L

E

-

L

L

A

G

G

G

I

V

L

T

P

A

-

G

F

I

H

H

F

V

V

T

L

V

T

A

C

A

P

P

E

E

G

G

Y

F

L

L

P

P

D

D

E

P

T

S

L

V

L

R

K

A

Q

A

A

A

R

E

A

R

N

R

A

A

K

Q

S

D

V

T

-

G

-

A

-

S

I

V

E

T

I

V

V

T

H

A

D

D

P

A

M

H

E

A

A

A

K

A

D

G

A

A

D

D

V

V

L

L

Y

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Q

Q

E

E

D

N

E

D

M

G

A

L

E

D

R

R

L

V

K

K

T

P

F

F

E

R

P

P

Y

F

Q

Q

I

L

N

N

S

S

K

R

M

L

A

A

E

L

A

A

K

D

S

S

S

D

A

A

V

I

V

V

M

L

H

H

C
|
C

M

L

P

P

A

A

H

H

R

R

G

G

Q

D

E

E

I

I

S

T

A

D

E

A

V

V

M

M

D

D

G

G

P

P

Q

A

S

S

I

A

I

V

I

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

V

V

K

W

L

L

M

L

active site: R102 (= R114), H129 (= H141), Q132 (= Q144), D225 (= D233), C265 (= C273), R293 (= R301)
binding 2-fluoro-4-hydroxybenzonitrile: T52 (≠ L64), F56 (≠ V68), T77 (≠ I89), L79 (≠ V91)

7nnyA Crystal structure of mycobacterium tuberculosis argf in complex with naphthalen-1-ol.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:305/308 of 7nnyA

query
sites
7nnyA

R

R

P

P

I

L

R

Q

H

G

-

P

K

R

T

G

V

V

A

A

M

V

I

I

F

F

N

D

K

K

P

N

S

S

L
x
T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

E

E

L

L

G

G

V

I

Y

A

E

Q

L

L

G

G

H

H

A

A

I

V

S

V

L

V

E

D

G

S

K

G

S

S

I

T

G

Q

V

L

G

G

E

R

R

D

E
|
E

S

T

V

L

E

Q

D

D

I

T

A

A

R

K

L
x
V

L

L

S

S

R

R

Y
|
Y

N

V

D

D

A

A

I

I

V

V

A

W

R
|
R

L

T

H

F

E

G

H

Q

E

E

I

R

I

L

E

D

T

A

L

M

A

A

A

S

H

V

A

A

S

T

I

V

P

P

V

V

I

I

N

N

A

A

L

L

T

S

D

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

L

L

A

A

D

D

A

L

F

Q

T

T

L

I

Y

A

E

E

-

R

K

K

G

G

L

A

W

L

R

R

D

G

D

-

I

L

K

R

V

L

V

S

F

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

A

A

N

H

S

S

W

L

I

L

E

-

L

L

A

G

G

G

I

V

L

T

P

A

-

G

F

I

H

H

F

V

V

T

L

V

T

A

C

A

P

P

E

E

G

G

Y

F

L

L

P

P

D

D

E

P

T

S

L

V

L

R

K

A

Q

A

A

A

R

E

A

R

N

R

A

A

K

Q

S

D

V

T

-

G

-

A

-

S

I

V

E

T

I

V

V

T

H

A

D

D

P

A

M

H

E

A

A

A

K

A

D

G

A

A

D

D

V

V

L

L

Y

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Q

Q

E

E

D

N

E

D

M

G

A

L

E

D

R

R

L

V

K

K

T

P

F

F

E

R

P

P

Y

F

Q

Q

I

L

N

N

S

S

K

R

M

L

A

A

E

L

A

A

K

D

S

S

S

D

A

A

V

I

V

V

M

L

H

H

C
|
C

M

L

P

P

A

A

H

H

R

R

G

G

Q

D

E

E

I

I

S

T

A

D

E

A

V

V

M

M

D

D

G

G

P

P

Q

A

S

S

I

A

I

V

I

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

V

V

K

W

L

L

M

L

active site: R102 (= R114), H129 (= H141), Q132 (= Q144), D225 (= D233), C265 (= C273), R293 (= R301)
binding 1-naphthol: T52 (≠ L64), R53 (= R65), F56 (≠ V68), E83 (= E95), V91 (≠ L103), Y95 (= Y107)

7nnwA Crystal structure of mycobacterium tuberculosis argf in complex with methyl 4-hydroxy-3-iodobenzoate.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:305/308 of 7nnwA

query
sites
7nnwA

R

R

P

P

I

L

R

Q

H

G

-

P

K

R

T

G

V

V

A

A

M

V

I
|
I

F

F

N

D

K

K

P

N

S

S

L
x
T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

E

E

L

L

G

G

V

I

Y

A

E

Q

L

L

G

G

H

H

A

A

I

V

S

V

L

V

E

D

G

S

K

G

S

S

I

T

G

Q

V
x
L

G

G

E

R

R

D

E

E

S

T

V

L

E

Q

D

D

I

T

A

A

R

K

L

V

L
|
L

S

S

R

R

Y
|
Y

N

V

D

D

A

A

I

I

V

V

A

W

R
|
R

L

T

H

F

E

G

H

Q

E

E

I

R

I

L

E

D

T

A

L

M

A

A

A

S

H

V

A

A

S

T

I

V

P

P

V

V

I

I

N

N

A

A

L

L

T

S

D

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

L

L

A

A

D

D

A

L

F

Q

T

T

L

I

Y

A

E

E

-

R

K

K

G

G

L

A

W

L

R

R

D

G

D

-

I

L

K

R

V

L

V

S

F

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

A

A

N

H

S

S

W

L

I

L

E

-

L

L

A

G

G

G

I

V

L

T

P

A

-

G

F

I

H

H

F

V

V

T

L

V

T

A

C

A

P

P

E

E

G

G

Y

F

L

L

P

P

D

D

E

P

T

S

L

V

L

R

K

A

Q

A

A

A

R

E

A

R

N

R

A

A

K

Q

S

D

V

T

-

G

-

A

-

S

I

V

E

T

I

V

V

T

H

A

D

D

P

A

M

H

E

A

A

A

K

A

D

G

A

A

D

D

V

V

L

L

Y

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Q

Q

E

E

D

N

E

D

M

G

A

L

E

D

R

R

L

V

K

K

T

P

F

F

E

R

P

P

Y

F

Q

Q

I

L

N

N

S

S

K

R

M

L

A

A

E

L

A

A

K

D

S

S

S

D

A

A

V

I

V

V

M

L

H

H

C
|
C

M

L

P

P

A

A

H

H

R

R

G

G

Q

D

E

E

I

I

S

T

A

D

E

A

V

V

M

M

D

D

G

G

P

P

Q

A

S

S

I

A

I

V

I

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

V

V

K

W

L

L

M

L

active site: R102 (= R114), H129 (= H141), Q132 (= Q144), D225 (= D233), C265 (= C273), R293 (= R301)
binding methyl 3-iodanyl-4-oxidanyl-benzoate: I46 (= I58), T52 (≠ L64), R53 (= R65), F56 (≠ V68), L79 (≠ V91), L92 (= L104), Y95 (= Y107)

7nnvA Crystal structure of mycobacterium tuberculosis argf in complex with carbamoyl phosphate.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:305/308 of 7nnvA

query
sites
7nnvA

R

R

P

P

I

L

R

Q

H

G

-

P

K

R

T

G

V

V

A

A

M

V

I

I

F

F

N

D

K

K

P

N

S
|
S

L
x
T

R
|
R

T
|
T

R

R

V

F

S

S

F

F

E

E

L

L

G

G

V

I

Y

A

E

Q

L

L

G

G

H

H

A

A

I

V

S

V

L

V

E

D

G

S

K

G

S

S

I

T

G

Q

V

L

G

G

E

R

R

D

E

E

S

T

V

L

E

Q

D

D

I

T

A

A

R

K

L

V

L

L

S

S

R

R

Y

Y

N

V

D

D

A

A

I

I

V

V

A

W

R
|
R

L

T

H

F

E

G

H

Q

E

E

I

R

I

L

E

D

T

A

L

M

A

A

A

S

H

V

A

A

S

T

I

V

P

P

V

V

I

I

N

N

A

A

L

L

T

S

D

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

L

L

A

A

D

D

A

L

F

Q

T

T

L

I

Y

A

E

E

-

R

K

K

G

G

L

A

W

L

R

R

D

G

D

-

I

L

K

R

V

L

V

S

F

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

A

A

N

H

S

S

W

L

I

L

E

-

L

L

A

G

G

G

I

V

L

T

P

A

-

G

F

I

H

H

F

V

V

T

L

V

T

A

C

A

P

P

E

E

G

G

Y

F

L

L

P

P

D

D

E

P

T

S

L

V

L

R

K

A

Q

A

A

A

R

E

A

R

N

R

A

A

K

Q

S

D

V

T

-

G

-

A

-

S

I

V

E

T

I

V

V

T

H

A

D

D

P

A

M

H

E

A

A

A

K

A

D

G

A

A

D

D

V

V

L

L

Y

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Q

Q

E

E

D

N

E

D

M

G

A

L

E

D

R

R

L

V

K

K

T

P

F

F

E

R

P

P

Y

F

Q

Q

I

L

N

N

S

S

K

R

M

L

A

A

E

L

A

A

K

D

S

S

S

D

A

A

V

I

V

V

M

L

H

H

C
|
C

M
x
L

P

P

A

A

H

H

R

R

G

G

Q

D

E

E

I

I

S

T

A

D

E

A

V

V

M

M

D

D

G

G

P

P

Q

A

S

S

I

A

I

V

I

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

V

V

K

W

L

L

M

L

active site: R102 (= R114), H129 (= H141), Q132 (= Q144), D225 (= D233), C265 (= C273), R293 (= R301)
binding phosphoric acid mono(formamide)ester: S51 (= S63), T52 (≠ L64), R53 (= R65), T54 (= T66), R102 (= R114), H129 (= H141), C265 (= C273), L266 (≠ M274), R293 (= R301)

2i6uA Crystal structure of ornithine carbamoyltransferase complexed with carbamoyl phosphate and l-norvaline from mycobacterium tuberculosis (rv1656) at 2.2 a (see paper)
48% identity, 79% coverage: 48:313/336 of query aligns to 34:304/307 of 2i6uA

query
sites
2i6uA

R

R

P

P

I

L

R

Q

H

G

-

P

K

R

T

G

V

V

A

A

M

V

I

I

F

F

N

D

K

K

P

N

S
|
S

L
x
T

R
|
R

T
|
T

R

R

V

F

S

S

F

F

E

E

L

L

G

G

V

I

Y

A

E

Q

L

L

G

G

H

H

A

A

I

V

S

V

L

V

E

D

G

S

K

G

S

S

I

T

G

Q

V

L

G

G

E
x
R

R

D

E

E

S

T

V

L

E

Q

D

D

I

T

A

A

R

K

L

V

L

L

S

S

R

R

Y

Y

N

V

D

D

A

A

I

I

V

V

A

W

R
|
R

L

T

H

F

E

G

H

Q

E

E

I

R

I

L

E

D

T

A

L

M

A

A

A

S

H

V

A

A

S

T

I

V

P

P

V

V

I

I

N

N

A

A

L
|
L

T

S

D

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

L

L

A

A

D

D

A

L

F

Q

T

T

L

I

Y

A

E

E

-

R

K

K

G

G

L

A

W

L

R

R

D

G

D

-

I

L

K

R

V

L

V

S

F

Y

V

F

G

G

D

D

G

G

-

A

N

N

N
|
N

V

M

A

A

N

H

S

S

W

L

I

L

E

-

L

L

A

G

G

G

I

V

L

T

P

A

-

G

F

I

H

H

F

V

V

T

L

V

T

A

C

A

P

P

E

E

G

G

Y

F

L

L

P

P

D

D

E

P

T

S

L

V

L

R

K

A

Q

A

A

A

R

E

A

R

N

R

A

A

K

Q

S

D

V

T

-

G

-

A

-

S

I

V

E

T

I

V

V

T

H

A

D

D

P

A

M

H

E

A

A

A

K

A

D

G

A

A

D

D

V

V

L

L

Y

V

T

T

D
|
D

V

T

W

W

T

T

S
|
S

M
|
M

G

G

Q

Q

E

E

D

N

E

D

M

G

A

L

E

D

R

R

L

V

K

K

T

P

F

F

E

R

P

P

Y

F

Q

Q

I

L

N

N

S

S

K

R

M

L

A

A

E

L

A

A

K

D

S

S

S

D

A

A

V

I

V

V

M

L

H

H

C
|
C

M
x
L

P

P

A

A

H

H

R

R

G

G

Q

D

E

E

I

I

S

T

A

D

E

A

V

V

M

M

D

D

G

G

P

P

Q

A

S

S

I

A

I

V

I

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

V

V

K

W

L

L

M

L

active site: R52 (= R65), T53 (= T66), R80 (≠ E93), R101 (= R114), H128 (= H141), Q131 (= Q144), D224 (= D233), C264 (= C273), R292 (= R301)
binding phosphoric acid mono(formamide)ester: S50 (= S63), T51 (≠ L64), R52 (= R65), T53 (= T66), R101 (= R114), C264 (= C273), L265 (≠ M274), R292 (= R301)
binding norvaline: L123 (= L136), N160 (= N172), D224 (= D233), S228 (= S237), M229 (= M238)

P9WIT9 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
48% identity, 79% coverage: 48:313/336 of query aligns to 34:304/307 of P9WIT9

query
sites
P9WIT9

R

R

P

P

I

L

R

Q

H

G

-

P

K

R

T

G

V

V

A

A

M

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I

I

F

F

N

D

K

K

P

N

S
|
S

L
x
T

R
|
R

T
|
T

R

R

V

F

S

S

F

F

E

E

L

L

G

G

V

I

Y

A

E

Q

L

L

G

G

H

H

A

A

I

V

S

V

L

V

E

D

G

S

K

G

S

S

I

T

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x
Q

V

L

G

G

E

R

R

D

E

E

S

T

V

L

E

Q

D

D

I

T

A

A

R

K

L

V

L

L

S

S

R

R

Y

Y

N

V

D

D

A

A

I

I

V

V

A

W

R
|
R

L

T

H

F

E

G

H

Q

E

E

I

R

I

L

E

D

T

A

L

M

A

A

A

S

H

V

A

A

S

T

I

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P

P

V

V

I

I

N

N

A

A

L

L

T

S

D

D

L

E

S

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H
|
H

P
|
P

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|
C

Q
|
Q

I

V

L

L

A

A

D

D

A

L

F

Q

T

T

L

I

Y

A

E

E

-

R

K

K

G

G

L

A

W

L

R

R

D

G

D

-

I

L

K

R

V

L

V

S

F

Y

V

F

G

G

D

D

G

G

-

A

N

N

N
|
N

V

M

A

A

N

H

S

S

W

L

I

L

E

-

L

L

A

G

G

G

I

V

L

T

P

A

-

G

F

I

H

H

F

V

V

T

L

V

T

A

C

A

P

P

E

E

G

G

Y

F

L

L

P

P

D

D

E

P

T

S

L

V

L

R

K

A

Q

A

A

A

R

E

A

R

N

R

A

A

K

Q

S

D

V

T

-

G

-

A

-

S

I

V

E

T

I

V

V

T

H

A

D

D

P

A

M

H

E

A

A

A

K

A

D

G

A

A

D

D

V

V

L

L

Y

V

T

T

D
|
D

V

T

W

W

T

T

S
|
S

M
|
M

G

G

Q

Q

E

E

D

N

E

D

M

G

A

L

E

D

R

R

L

V

K

K

T

P

F

F

E

R

P

P

Y

F

Q

Q

I

L

N

N

S

S

K

R

M

L

A

A

E

L

A

A

K

D

S

S

S

D

A

A

V

I

V

V

M

L

H

H

C
|
C

M
x
L

P

P

A

A

H

H

R

R

G

G

Q

D

E

E

I

I

S

T

A

D

E

A

V

V

M

M

D

D

G

G

P

P

Q

A

S

S

I

A

I

V

I

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

V

V

K

W

L

L

M

L

STRT 50:53 (≠ SLRT 63:66) binding carbamoyl phosphate
Q77 (≠ G90) binding carbamoyl phosphate
R101 (= R114) binding carbamoyl phosphate
HPCQ 128:131 (= HPCQ 141:144) binding carbamoyl phosphate
N160 (= N172) binding L-ornithine
D224 (= D233) binding L-ornithine
SM 228:229 (= SM 237:238) binding L-ornithine
CL 264:265 (≠ CM 273:274) binding carbamoyl phosphate
R292 (= R301) binding carbamoyl phosphate

7np0A Crystal structure of mycobacterium tuberculosis argf in complex with (4-nitrophenyl)boronic acid.
47% identity, 82% coverage: 39:313/336 of query aligns to 26:302/305 of 7np0A

query
sites
7np0A

E

E

A

L

N

K

R

K

Q

D

T

P

A

V

E

S

F

R

R

R

P

P

I

L

R

Q

H

G

-

P

K

R

T

G

V

V

A

A

M

V

I

I

F

F

N

D

K

K

P

N

S

S

L

T

R

R

T

T

R

R

V

F

S

S

F

F

E

E

L

L

G

G

V

I

Y

A

E

Q

L

L

G

G

H

H

A

A

I

V

S

V

L

V

E

D

G

S

K

-

S

-

I

-

G

G

V

L

G

G

E

R

R

D

E
|
E

S

T

V

L

E

Q

D

D

I

T

A

A

R

K

L
x
V

L

L

S

S

R

R

Y
|
Y

N

V

D

D

A

A

I

I

V

V

A

W

R
|
R

L

T

H

F

E

G

H

Q

E

E

I

R

I

L

E

D

T

A

L

M

A

A

A

S

H

V

A

A

S

T

I

V

P

P

V

V

I

I

N

N

A

A

L

L

T

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D

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

L

L

A

A

D

D

A

L

F

Q

T

T

L

I

Y

A

E

E

-

R

K

K

G

G

L

A

W

L

R

R

D

G

D

-

I

L

K

R

V

L

V

S

F

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

A

A

N

H

S

S

W

L

I

L

E

-

L

L

A

G

G

G

I

V

L

T

P

A

-

G

F

I

H

H

F

V

V

T

L

V

T

A

C

A

P

P

E

E

G

G

Y

F

L

L

P

P

D

D

E

P

T

S

L

V

L

R

K

A

Q

A

A

A

R

E

A

R

N

R

A

A

K

Q

S

D

V

T

-

G

-

A

-

S

I

V

E

T

I

V

V

T

H

A

D

D

P

A

M

H

E

A

A

A

K

A

D

G

A

A

D

D

V

V

L

L

Y

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Q

Q

E

E

D

N

E

D

M

G

A

L

E

D

R

R

L

V

K

K

T

P

F

F

E

R

P

P

Y

F

Q

Q

I

L

N

N

S

S

K

R

M

L

A

A

E

L

A

A

K

D

S

S

S

D

A

A

V

I

V

V

M

L

H

H

C
|
C

M

L

P

P

A

A

H

H

R

R

G

G

Q

D

E

E

I

I

S

T

A

D

E

A

V

V

M

M

D

D

G

G

P

P

Q

A

S

S

I

A

I

V

I

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

V

V

K

W

L

L

M

L

active site: R99 (= R114), H126 (= H141), Q129 (= Q144), D222 (= D233), C262 (= C273), R290 (= R301)
binding p-nitrophenylboronic acid: E80 (= E95), V88 (≠ L103), Y92 (= Y107)

7novA Crystal structure of mycobacterium tuberculosis argf in complex with (4-methyl-3-nitrophenyl)boronic acid.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:299/302 of 7novA

query
sites
7novA

R

R

P

P

I

L

R

Q

H

G

-

P

K

R

T

G

V

V

A

A

M

V

I

I

F

F

N

D

K

K

P

N

S

S

L

T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

E

E

L

L

G

G

V

I

Y

A

E

Q

L

L

G

G

H

H

A

A

I

V

S

V

L

V

E

D

G

S

K

R

S

D

I

-

G

-

V

-

G

-

E

-

R

-

E
|
E

S

T

V

L

E

Q

D

D

I

T

A

A

R

K

L
x
V

L

L

S

S

R

R

Y
|
Y

N

V

D

D

A

A

I

I

V

V

A

W

R
|
R

L

T

H

F

E

G

H

Q

E

E

I

R

I

L

E

D

T

A

L

M

A

A

A

S

H

V

A

A

S

T

I

V

P

P

V

V

I

I

N

N

A

A

L

L

T

S

D

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

L

L

A

A

D

D

A

L

F

Q

T

T

L

I

Y

A

E

E

-

R

K

K

G

G

L

A

W

L

R

R

D

G

D

-

I

L

K

R

V

L

V

S

F

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

A

A

N

H

S

S

W

L

I

L

E

-

L

L

A

G

G

G

I

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L

T

P

A

-

G

F

I

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H

F

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T

L

V

T

A

C

A

P

P

E

E

G

G

Y

F

L

L

P

P

D

D

E

P

T

S

L

V

L

R

K

A

Q

A

A

A

R

E

A

R

N

R

A

A

K

Q

S

D

V

T

-

G

-

A

-

S

I

V

E

T

I

V

V

T

H

A

D

D

P

A

M

H

E

A

A

A

K

A

D

G

A

A

D

D

V

V

L

L

Y

V

T

T

D
|
D

V

T

W

W

T

T

S

S

M

M

G

G

Q

Q

E

E

D

N

E

D

M

G

A

L

E

D

R

R

L

V

K

K

T

P

F

F

E

R

P

P

Y

F

Q

Q

I

L

N

N

S

S

K

R

M

L

A

A

E

L

A

A

K

D

S

S

S

D

A

A

V

I

V

V

M

L

H

H

C
|
C

M
x
L

P

P

A

A

H

H

R

R

G

G

Q

D

E

E

I

I

S

T

A

D

E

A

V

V

M

M

D

D

G

G

P

P

Q

A

S

S

I

A

I

V

I

W

D

D

E

E

A
|
A

E

E

N

N

R
|
R

L

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

V

V

K

W

L

L

M

L

active site: R96 (= R114), H123 (= H141), Q126 (= Q144), D219 (= D233), C259 (= C273), R287 (= R301)
binding (4-methyl-3-nitro-phenyl)-oxidanyl-oxidanylidene-boron: R53 (= R65), F56 (≠ V68), E77 (= E95), V85 (≠ L103), Y89 (= Y107), L260 (≠ M274), A284 (= A298), R287 (= R301)

7nnzB Crystal structure of mycobacterium tuberculosis argf in complex with 5-methyl-4-phenylthiazol-2-amine.
47% identity, 79% coverage: 48:313/336 of query aligns to 34:294/297 of 7nnzB

query
sites
7nnzB

R

R

P

P

I

L

R

Q

H

G

-

P

K

R

T

G

V

V

A

A

M

V

I

I

F

F

N

D

K

K

P

N

S

S

L
x
T

R
|
R

T

T

R

R

V
x
F

S

S

F

F

E

E

L

L

G

G

V

I

Y

A

E

Q

L

L

G

G

H

H

A

A

I

V

S

V

L

V

E

D

G

S

K

G

S

S

I

T

G

Q

V

L

G

G

E

R

R

D

E

E

S

T

V

L

E

Q

D

D

I

T

A

A

R

K

L

V

L

L

S

S

R

R

Y

Y

N

V

D

D

A

A

I

I

V

V

A

W

R
|
R

L

T

H

F

E

G

H

Q

E

E

I

R

I

L

E

D

T

A

L

M

A

A

A

S

H

V

A

A

S

T

I

V

P

P

V

V

I

I

N

N

A

A

L

L

T

S

D

D

L

E

S

F

H
|
H

P

P

C

C

Q
|
Q

I

V

L

L

A

A

D

D

A

L

F

Q

T

T

L

I

Y

A

E

E

-

R

K

K

G

G

L

A

W

L

R

R

D

-

D

G

I

L

K

R

V

L

V

S

F

Y

V

F

G

G

D

D

G

G

-

A

N

N

V

M

A

A

N

H

S

S

W

L

I

L

E

-

L

L

A

G

G

G

I

V

L

T

P

A

-

G

F

I

H

H

F

V

V

T

L

V

T

A

C

A

P

P

E

E

G

G

Y

F

L

L

P

P

D

D

E

P

T

S

L

V

L

R

K

A

Q

A

A

A

R

E

A

R

N

R

A

A

K

Q

S

D

V

T

-

G

-

A

-

S

I

V

E

T

I

V

V

T

H

A

D

D

P

A

M

H

E

A

A

A

K

A

D

G

A

A

D

D

V

V

L

L

Y

V

T

T

D
|
D

V

T

W

W

T

T

S

-

M

-

G

-

Q

-

E

-

D

-

E

-

M

-

A

-

E

-

R

R

L

V

K

K

T

P

F

F

E

R

P

P

Y

F

Q

Q

I

L

N

N

S

S

K

R

M

L

A

A

E

L

A

A

K

D

S

S

S

D

A

A

V

I

V

V

M

L

H

H

C
|
C

M

L

P

P

A

A

H

H

R

R

G

G

Q

D

E

E

I

I

S

T

A

D

E

A

V

V

M

M

D

D

G

G

P

P

Q

A

S

S

I

A

I

V

I

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

V

A

Q

Q

K

K

A

A

L

L

M

L

V

V

K

W

L

L

M

L

active site: R101 (= R114), H128 (= H141), Q131 (= Q144), D224 (= D233), C254 (= C273), R282 (= R301)
binding 5-methyl-4-phenyl-1,3-thiazol-2-amine: T51 (≠ L64), R52 (= R65), F55 (≠ V68)

P00481 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Rattus norvegicus (Rat) (see 2 papers)
38% identity, 93% coverage: 3:314/336 of query aligns to 32:343/354 of P00481

query
sites
P00481

K
x
Q

Q

S

Q

Q

T

V

G

Q

T

L

K

K

K

G

R

R

D

D

F

L

L

L

G

T

F

L

T

K

G

N

L

F

D

T

A

G

E

E

K

E

I

I

I

Q

E

Y

L

M

F

L

D

W

Y

L

S

S

L

A

H

D

I

L

K

K

Q

F

Q

R

R

I

E

-

A

-

N

-

R

K

Q

Q

T

K

A

G

E

E

F

Y

R

L

P

P

I

L

-

L

R

Q

H

G

K

K

T

S

V

L

A

G

M

M

I

I

F

F

N

E

K

K

P

R

S

S

L

T

R
|
R

T

T

R

R

V

L

S

S

F

T

E

E

L

T

G

G

V

F

Y

A

E

L

L

L

G

G

H

H

A

P

I

S

S

F

L

L

E

T

G

T

K

Q

S

D

I

I

G

H

V

L

G

G

E

V

R

N

E

E

S

S

V

L

E

T

D

D

I

T

A

A

R

R

L

V

L

L

S

S

R

S

Y

M

N

T

D

D

A

A

I

V

V

L

A

A

R

R

L

V

H

Y

E

K

H

Q

E

S

I

D

I

L

E

D

T

I

L

L

A

A

A

K

H

E

A

A

S

T

I

I

P

P

V

I

I

V

N

N

A

G

L

L

T

S

D

D

L

L

S

Y

H

H

P

P

C

I

Q

Q

I

I

L

L

A

A

D

D

A

Y

F

L

T

T

L

L

Y

Q

E

E

K

H

G

Y

L

G

W

S

R

L

D

K

D

G

I

L

K

T

V

L

V

S

F

W

V

I

G

G

D

D

G

G

N

N

V

I

A

L

N

H

S

S

W

I

I

M

E

M

L

S

A

A

G

A

I

K

L

F

P

G

F

M

H

H

F

L

V

Q

L

A

T

A

C

T

P

P

E

K

G

G

Y

Y

L

E

P

P

D

D

E

P

T

N

L

I

L

V

K

K

Q

L

A

A

R

E

A

Q

N

Y

A

A

K

K

-

E

-

N

-

G

S

T

V

R

I

L

E

S

I

M

V

T

H

N

D

D

P

P

M

L

E

E

A

A

K

R

D

G

A

G

D

N

V

V

L

L

Y

I

T

T

D

D

V

T

W

W

T

I

S

S

M

M

G

G

Q

Q

E

E

D

D

E

E

M

K

A

K

E

K

R

R

L

L

K

Q

T

A

F

F

E

Q

P

G

Y

Y

Q

Q

I

V

N

T

S

M

K

K

M

T

M

A

A

K

E

V

A

A

K

A

S

S

S

D

A

W

V

T

V

F

M

L

H

H

C
|
C

M

L

P

P

A

-

H

R

R

K

G

P

Q

E

E

E

I

V

S

D

A

D

E

E

V

V

M

F

D

Y

G

S

P

P

Q

R

S

S

I

L

I

V

I

F

D

P

E

E

A

A

E

E

N

N

R

R

L

K

H

W

V

T

Q

I

K

M

A

A

L

V

M

M

V

V

K

S

L

L

M

L

N

T

R92 (= R65) mutation to L: Strong decrease in ornithine carbamoyltransferase activity.
C303 (= C273) mutation to S: Increases KM for ornithine 5-fold and decreases kcat 20-fold.

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

P00480 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Homo sapiens (Human) (see 31 papers)
39% identity, 91% coverage: 9:313/336 of query aligns to 38:342/354 of P00480

query
sites
P00480

K

K

K
x
G

R
|
R

D

D

F

L

L
|
L

G

T

F

L

T
x
K

G

N

L

F

D

T

A

G

E

E

K

E

I

I

I

K

E
x
Y

L

M

F

L

D

W

Y

L

S

S

L

A

H

D

I
x
L

K

K

Q

F

Q

R

R

I

E

-

A

-

N

-

R

K

Q

Q

T

K

A

G

E

E

F

Y

R

L

P

P

I

L

-

L

R

Q

H

G

K

K

T

S

V

L

A

G

M

M

I

I

F

F

N

E

K
|
K

P

R

S
|
S

L
x
T

R
|
R

T
|
T

R

R

V

L

S

S

F

T

E

E

L

T

G
|
G

V
x
F

Y

A

E

L

L

L

G

G

H

H

A

P

I

C

S

F

L
|
L

E

T

G

T

K

Q

S

D

I

I

G

H

V

L

G

G

E

V

R

N

E

E

S

S

V

L

E
x
T

D
|
D

I

T

A

A

R
|
R

L

V

L

L

S

S

R

S

Y

M

N

A

D

D

A

A

I

V

V

L

A
|
A

R
|
R

L

V

H

Y

E

K

H

Q

E

S

I

D

I

L

E

D

T

T

L

L

A

A

A

K

H

E

A

A

S

S

I

I

P

P

V

I

I

I

N

N

A

G

L

L

T

S

D

D

L

L

S

Y

H
|
H

P

P

C

I

Q
|
Q

I
|
I

L

L

A

A

D

D

A
x
Y

F

L

T
|
T

L
|
L

Y

Q

E

E

K

H

G
x
Y

L

S

W

S

R

L

D

K

D
x
G

I

L

K

T

V

L

V

S

F

W

V

I

G
|
G

D
|
D

G

G

N

N

V

I

A
x
L

N

H

S

S

W

I

I

M

E

M

L
x
S

A

A

G
x
A

I

K

L

F

P

G

F
x
M

H
|
H

F

L

V

Q

L

A

T

A

C

T

P
|
P

E

K

G

G

Y

Y

L

E

P
|
P

D

D

E

A

T

S

L

V

L

T

K

K

Q

L

A

A

R

E

A

Q

N

Y

A

A

K

K

-

E

-

N

-

G

S

T

V

K

I
x
L

E

L

I

L

V

T

H

N

D

D

P

P

M

L

E

E

A

A

K

H

D

G

A

G

D

N

V

V

L

L

Y

I

T
|
T

D

D

V
x
T

W
|
W

T

I

S

S

M

M

G
|
G

Q
|
Q

E

E

D
x
E

E

E

M

K

A

K

E

K

R
|
R

L

L

K

Q

T

A

F

F

E

Q

P

G

Y

Y

Q

Q

I

V

N

T

S

M

K

K

M

T

M

A

A

K

E

V

A

A

K

A

S

S

S

D

A

W

V

T

V

F

M

L

H
|
H

C
|
C

M
x
L

P

P

A

-

H

R

R

K

G

P

Q
x
E

E

E

I

V

S

D

A

D

E

E

V

V

M

F

D

Y

G

S

P

P

Q

R

S

S

I

L

I

V

I

F

D

P

E

E

A

A

E

E

N

N

R
|
R

L

K

H

W

V
x
T

Q

I

K

M

A

A

L

V

M

M

V

V

K
x
S

L

L

M

L

G39 (≠ K10) to C: in OTCD; late onset; dbSNP:rs72554306
R40 (= R11) to H: in OTCD; late onset; dbSNP:rs72554308
L43 (= L14) to F: in dbSNP:rs72554309
K46 (≠ T17) to R: in dbSNP:rs1800321
Y55 (≠ E26) to D: in OTCD; late onset; dbSNP:rs72554319
L63 (≠ I34) to P: in OTCD; late onset; dbSNP:rs72554324
K88 (= K61) modified: N6-acetyllysine; alternate; to N: in OTCD; late onset; dbSNP:rs72554339
STRT 90:93 (≠ SLRT 63:66) binding carbamoyl phosphate
G100 (= G73) to D: in OTCD; late onset; dbSNP:rs72554349
F101 (≠ V74) to L: in dbSNP:rs1133135
L111 (= L84) to P: in dbSNP:rs1800324
T125 (≠ E98) to M: in OTCD; neonatal; dbSNP:rs72554356
D126 (= D99) to G: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; 0.9% of wild-type activity; dbSNP:rs72554358
R129 (= R102) to H: in OTCD; early onset; decreased ornithine carbamoyltransferase activity; 2.1% of wild-type activity; dbSNP:rs66656800
A140 (= A113) to P: in OTCD; late onset; dbSNP:rs72556260
R141 (= R114) binding carbamoyl phosphate; to Q: in OTCD; most common variant; loss of ornithine carbamoyltransferase activity; activity is 100-fold lower; dbSNP:rs68026851
H168 (= H141) binding carbamoyl phosphate
Q171 (= Q144) binding carbamoyl phosphate
I172 (= I145) to M: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; dbSNP:rs72556280
Y176 (≠ A149) to C: in OTCD; late onset; dbSNP:rs72556283
TL 178:179 (= TL 151:152) natural variant: Missing (in OTCD; neonatal)
Y183 (≠ G156) to D: in OTCD; late onset; dbSNP:rs72556292
G188 (≠ D161) to R: in OTCD; neonatal; dbSNP:rs72556294
G195 (= G168) to R: in OTCD; loss of ornithine carbamoyltransferase activity; dbSNP:rs67294955
D196 (= D169) to V: in OTCD; neonatal; decreased ornithine carbamoyltransferase activity; 3.7% activity; dbSNP:rs72556300
L201 (≠ A174) to P: in OTCD; neonatal; dbSNP:rs72558407
S207 (≠ L180) to R: in OTCD; neonatal; dbSNP:rs72558415
A209 (≠ G182) to V: in OTCD; neonatal; dbSNP:rs72558417
M213 (≠ F186) to K: in OTCD; late onset
H214 (= H187) to Y: in OTCD; neonatal; dbSNP:rs72558420
P220 (= P193) to A: in OTCD; late onset; dbSNP:rs72558425
P225 (= P198) to T: in OTCD; late onset; dbSNP:rs72558428
L244 (≠ I214) to Q: in OTCD; late onset; dbSNP:rs72558436
T262 (= T232) to K: in OTCD; mild; dbSNP:rs67333670
T264 (≠ V234) to A: in OTCD; late onset; decreased ornithine carbamoyltransferase activity; 8.9% activity; dbSNP:rs72558444; to I: in OTCD; late onset; dbSNP:rs67156896
W265 (= W235) to L: in OTCD; mild; dbSNP:rs72558446
G269 (= G239) to E: in OTCD; neonatal; dbSNP:rs72558450
Q270 (= Q240) to R: in dbSNP:rs1800328
E272 (≠ D242) natural variant: Missing (in OTCD; late onset; dbSNP:rs72558452)
R277 (= R247) to Q: in OTCD; late onset; dbSNP:rs66724222; to W: in OTCD; late onset; dbSNP:rs72558454
H302 (= H272) to L: in OTCD; female; late onset; dbSNP:rs67993095; to Y: in OTCD; neonatal; dbSNP:rs72558463
C303 (= C273) to R: in OTCD; neonatal; dbSNP:rs67468335
CL 303:304 (≠ CM 273:274) binding carbamoyl phosphate
E309 (≠ Q280) natural variant: Missing (in OTCD; late onset)
R330 (= R301) binding carbamoyl phosphate
T333 (≠ V304) natural variant: T -> A
S340 (≠ K311) to P: in OTCD; late onset; dbSNP:rs72558489

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion
15 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-23 and G-26.
23 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-26.
26 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-23.
343 T → K: in OTCD; late onset; dbSNP:rs72558491

1othA Crystal structure of human ornithine transcarbamoylase complexed with n-phosphonacetyl-l-ornithine (see paper)
39% identity, 91% coverage: 9:314/336 of query aligns to 5:310/321 of 1othA

query
sites
1othA

K

K

K

G

R

R

D

D

F

L

L

L

G

T

F

L

T

K

G

N

L

F

D

T

A

G

E

E

K

E

I

I

I

K

E

Y

L

M

F

L

D

W

Y

L

S

S

L

A

H

D

I

L

K

K

Q

F

Q

R

R

I

E

-

A

-

N

-

R

K

Q

Q

T

K

A

G

E

E

F

Y

R

L

P

P

I

L

-

L

R

Q

H

G

K

K

T

S

V

L

A

G

M

M

I

I

F

F

N

E

K

K

P

R

S
|
S

L
x
T

R
|
R

T
|
T

R

R

V

L

S

S

F

T

E

E

L

T

G

G

V

F

Y

A

E

L

L

L

G

G

H

H

A

P

I

C

S

F

L

L

E

T

G

T

K

Q

S

D

I

I

G

H

V

L

G

G

E
x
V

R

N

E

E

S

S

V

L

E

T

D

D

I

T

A

A

R

R

L

V

L

L

S

S

R

S

Y

M

N

A

D

D

A

A

I

V

V

L

A

A

R
|
R

L

V

H

Y

E

K

H

Q

E

S

I

D

I

L

E

D

T

T

L

L

A

A

A

K

H

E

A

A

S

S

I

I

P

P

V

I

I

I

N

N

A

G

L
|
L

T

S

D

D

L

L

S

Y

H
|
H

P

P

C

I

Q
|
Q

I

I

L

L

A

A

D

D

A

Y

F

L

T

T

L

L

Y

Q

E

E

K

H

G

Y

L

S

W

S

R

L

D

K

D

G

I

L

K

T

V

L

V

S

F

W

V

I

G

G

D

D

G

G

N

N

N
|
N

V

I

A

L

N

H

S

S

W

I

I

M

E

M

L

S

A

A

G

A

I

K

L

F

P

G

F

M

H

H

F

L

V

Q

L

A

T

A

C

T

P

P

E

K

G

G

Y

Y

L

E

P

P

D

D

E

A

T

S

L

V

L

T

K

K

Q

L

A

A

R

E

A

Q

N

Y

A

A

K

K

-

E

-

N

-

G

S

T

V

K

I

L

E

L

I

L

V

T

H

N

D

D

P

P

M

L

E

E

A

A

K

H

D

G

A

G

D

N

V

V

L

L

Y

I

T

T

D
|
D

V

T

W

W

T

I

S
|
S

M
|
M

G

G

Q

R

E

E

D

E

E

E

M

K

A

K

E

K

R

R

L

L

K

Q

T

A

F

F

E

Q

P

G

Y

Y

Q

Q

I

V

N

T

S

M

K

K

M

T

M

A

A

K

E

V

A

A

K

A

S

S

S

D

A

W

V

T

V

F

M

L

H

H

C
|
C

M
x
L

P

P

A

-

H

R

R

K

G

P

Q

E

E

E

I

V

S

D

A

D

E

E

V

V

M

F

D

Y

G

S

P

P

Q

R

S

S

I

L

I

V

I

F

D

P

E

E

A

A

E

E

N

N

R
|
R

L

K

H

W

V

T

Q

I

K

M

A

A

L

V

M

M

V

V

K

S

L

L

M

L

N

T

active site: R59 (= R65), T60 (= T66), V87 (≠ E93), R108 (= R114), H135 (= H141), Q138 (= Q144), D230 (= D233), C270 (= C273), R297 (= R301)
binding n-(phosphonoacetyl)-l-ornithine: S57 (= S63), T58 (≠ L64), R59 (= R65), T60 (= T66), R108 (= R114), L130 (= L136), H135 (= H141), N166 (= N172), D230 (= D233), S234 (= S237), M235 (= M238), C270 (= C273), L271 (≠ M274), R297 (= R301)

1c9yA Human ornithine transcarbamylase: crystallographic insights into substrate recognition and catalytic mechanism (see paper)
39% identity, 91% coverage: 9:314/336 of query aligns to 5:310/321 of 1c9yA

query
sites
1c9yA

K

K

K

G

R

R

D

D

F

L

L

L

G

T

F

L

T

K

G

N

L

F

D

T

A

G

E

E

K

E

I

I

I

K

E

Y

L

M

F

L

D

W

Y

L

S

S

L

A

H

D

I

L

K

K

Q

F

Q

R

R

I

E

-

A

-

N

-

R

K

Q

Q

T

K

A

G

E

E

F

Y

R

L

P

P

I

L

-

L

R

Q

H

G

K

K

T

S

V

L

A

G

M

M

I

I

F

F

N

E

K

K

P

R

S
|
S

L
x
T

R
|
R

T
|
T

R

R

V

L

S

S

F

T

E

E

L

T

G

G

V

F

Y

A

E

L

L

L

G

G

H

H

A

P

I

C

S

F

L

L

E

T

G

T

K

Q

S

D

I

I

G

H

V

L

G

G

E
x
V

R

N

E

E

S

S

V

L

E

T

D

D

I

T

A

A

R

R

L

V

L

L

S

S

R

S

Y

M

N

A

D

D

A

A

I

V

V

L

A

A

R
|
R

L

V

H

Y

E

K

H

Q

E

S

I

D

I

L

E

D

T

T

L

L

A

A

A

K

H

E

A

A

S

S

I

I

P

P

V

I

I

I

N

N

A

G

L
|
L

T

S

D

D

L

L

S

Y

H
|
H

P

P

C

I

Q
|
Q

I

I

L

L

A

A

D

D

A

Y

F

L

T

T

L

L

Y

Q

E

E

K

H

G

Y

L

S

W

S

R

L

D

K

D

G

I

L

K

T

V

L

V

S

F

W

V

I

G

G

D

D

G

G

N

N

N
|
N

V

I

A

L

N

H

S

S

W

I

I

M

E

M

L

S

A

A

G

A

I

K

L

F

P

G

F

M

H

H

F

L

V

Q

L

A

T

A

C

T

P

P

E

K

G

G

Y

Y

L

E

P

P

D

D

E

A

T

S

L

V

L

T

K

K

Q

L

A

A

R

E

A

Q

N

Y

A

A

K

K

-

E

-

N

-

G

S

T

V

K

I

L

E

L

I

L

V

T

H

N

D

D

P

P

M

L

E

E

A

A

K

H

D

G

A

G

D

N

V

V

L

L

Y

I

T

T

D
|
D

V

T

W

W

T

I

S
|
S

M
|
M

G

G

Q

R

E

E

D

E

E

E

M

K

A

K

E

K

R

R

L

L

K

Q

T

A

F

F

E

Q

P

G

Y

Y

Q

Q

I

V

N

T

S

M

K

K

M

T

M

A

A

K

E

V

A

A

K

A

S

S

S

D

A

W

V

T

V

F

M

L

H

H

C
|
C

M
x
L

P

P

A

-

H

R

R

K

G

P

Q

E

E

E

I

V

S

D

A

D

E

E

V

V

M

F

D

Y

G

S

P

P

Q

R

S

S

I

L

I

V

I

F

D

P

E

E

A

A

E

E

N

N

R
|
R

L

K

H

W

V

T

Q

I

K

M

A

A

L

V

M

M

V

V

K

S

L

L

M

L

N

T

active site: R59 (= R65), T60 (= T66), V87 (≠ E93), R108 (= R114), H135 (= H141), Q138 (= Q144), D230 (= D233), C270 (= C273), R297 (= R301)
binding phosphoric acid mono(formamide)ester: S57 (= S63), T58 (≠ L64), R59 (= R65), T60 (= T66), R108 (= R114), C270 (= C273), L271 (≠ M274), R297 (= R301)
binding norvaline: L130 (= L136), N166 (= N172), D230 (= D233), S234 (= S237), M235 (= M238)

Query Sequence

>WP_012502229.1 NCBI__GCF_000020505.1:WP_012502229.1
MSKQQTGTKKRDFLGFTGLDAEKIIELFDYSLHIKQQREANRQTAEFRPIRHKTVAMIFN
KPSLRTRVSFELGVYELGGHAISLEGKSIGVGERESVEDIARLLSRYNDAIVARLHEHEI
IETLAAHASIPVINALTDLSHPCQILADAFTLYEKGLWRDDIKVVFVGDGNNVANSWIEL
AGILPFHFVLTCPEGYLPDETLLKQARANAKSVIEIVHDPMEAAKDADVLYTDVWTSMGQ
EDEMAERLKTFEPYQINSKMMAEAKSSAVVMHCMPAHRGQEISAEVMDGPQSIIIDEAEN
RLHVQKALMVKLMNHDVYRKFHLTHRLNRAASQLKA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory