SitesBLAST
Comparing WP_012502229.1 NCBI__GCF_000020505.1:WP_012502229.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8qeuA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with ornithine (see paper)
46% identity, 90% coverage: 11:314/336 of query aligns to 2:303/304 of 8qeuA
Q51742 Ornithine carbamoyltransferase, anabolic; OTCase; EC 2.1.3.3 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see 3 papers)
47% identity, 90% coverage: 11:314/336 of query aligns to 8:311/315 of Q51742
- W22 (≠ I25) mutation to A: Decreased heat stability.
- E26 (≠ D29) mutation to Q: Increased dissociation of dodecamers into trimers.
- M30 (≠ H33) mutation to A: Increased dissociation of dodecamers into trimers.
- W34 (≠ Q37) mutation to A: Increased dissociation of dodecamers into trimers.
- Y228 (= Y231) mutation to C: Becomes active at low temperatures; when associated with G-278.
- A241 (≠ M244) mutation to D: Becomes active at low temperatures; when associated with G-278.
- E278 (= E281) mutation to G: Becomes active at low temperatures; when associated with C-228 or D-241.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
8qevA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with carbamoyl phosphate (see paper)
44% identity, 90% coverage: 11:314/336 of query aligns to 2:296/297 of 8qevA
Q81M99 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Bacillus anthracis
45% identity, 90% coverage: 11:313/336 of query aligns to 12:309/316 of Q81M99
- STRT 57:60 (≠ SLRT 63:66) binding carbamoyl phosphate
- Q84 (≠ G90) binding carbamoyl phosphate
- R108 (= R114) binding carbamoyl phosphate
- HPCQ 135:138 (= HPCQ 141:144) binding carbamoyl phosphate
- N166 (= N172) binding L-ornithine
- D230 (= D233) binding L-ornithine
- SM 234:235 (= SM 237:238) binding L-ornithine
- CL 269:270 (≠ CM 273:274) binding carbamoyl phosphate
- R297 (= R301) binding carbamoyl phosphate
4nf2A Crystal structure of anabolic ornithine carbamoyltransferase from bacillus anthracis in complex with carbamoyl phosphate and l- norvaline
44% identity, 90% coverage: 11:313/336 of query aligns to 8:305/307 of 4nf2A
- active site: R55 (= R65), T56 (= T66), R83 (≠ E93), R104 (= R114), H131 (= H141), Q134 (= Q144), D226 (= D233), C265 (= C273), R293 (= R301)
- binding phosphoric acid mono(formamide)ester: S53 (= S63), T54 (≠ L64), R55 (= R65), T56 (= T66), R104 (= R114), H131 (= H141), Q134 (= Q144), C265 (= C273), L266 (≠ M274), R293 (= R301)
- binding norvaline: L126 (= L136), N162 (= N172), D226 (= D233), S230 (= S237), M231 (= M238)
7nouA Crystal structure of mycobacterium tuberculosis argf in complex with (3,5-dichlorophenyl)boronic acid.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:305/308 of 7nouA
- active site: R102 (= R114), H129 (= H141), Q132 (= Q144), D225 (= D233), C265 (= C273), R293 (= R301)
- binding [3,5-bis(chloranyl)phenyl]-oxidanyl-oxidanylidene-boron: I46 (= I58), T52 (≠ L64), R53 (= R65), R53 (= R65), F56 (≠ V68), F56 (≠ V68), L79 (≠ V91), D82 (≠ R94), E83 (= E95), V91 (≠ L103), Y95 (= Y107), L266 (≠ M274), R293 (= R301)
7nosA Crystal structure of mycobacterium tuberculosis argf in complex with 4-bromo-6-(trifluoromethyl)-1h-benzo[d]imidazole.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:305/308 of 7nosA
7norA Crystal structure of mycobacterium tuberculosis argf in complex with 2-fluoro-4-hydroxybenzonitrile.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:305/308 of 7norA
7nnyA Crystal structure of mycobacterium tuberculosis argf in complex with naphthalen-1-ol.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:305/308 of 7nnyA
- active site: R102 (= R114), H129 (= H141), Q132 (= Q144), D225 (= D233), C265 (= C273), R293 (= R301)
- binding 1-naphthol: T52 (≠ L64), R53 (= R65), F56 (≠ V68), E83 (= E95), V91 (≠ L103), Y95 (= Y107)
7nnwA Crystal structure of mycobacterium tuberculosis argf in complex with methyl 4-hydroxy-3-iodobenzoate.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:305/308 of 7nnwA
- active site: R102 (= R114), H129 (= H141), Q132 (= Q144), D225 (= D233), C265 (= C273), R293 (= R301)
- binding methyl 3-iodanyl-4-oxidanyl-benzoate: I46 (= I58), T52 (≠ L64), R53 (= R65), F56 (≠ V68), L79 (≠ V91), L92 (= L104), Y95 (= Y107)
7nnvA Crystal structure of mycobacterium tuberculosis argf in complex with carbamoyl phosphate.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:305/308 of 7nnvA
- active site: R102 (= R114), H129 (= H141), Q132 (= Q144), D225 (= D233), C265 (= C273), R293 (= R301)
- binding phosphoric acid mono(formamide)ester: S51 (= S63), T52 (≠ L64), R53 (= R65), T54 (= T66), R102 (= R114), H129 (= H141), C265 (= C273), L266 (≠ M274), R293 (= R301)
2i6uA Crystal structure of ornithine carbamoyltransferase complexed with carbamoyl phosphate and l-norvaline from mycobacterium tuberculosis (rv1656) at 2.2 a (see paper)
48% identity, 79% coverage: 48:313/336 of query aligns to 34:304/307 of 2i6uA
- active site: R52 (= R65), T53 (= T66), R80 (≠ E93), R101 (= R114), H128 (= H141), Q131 (= Q144), D224 (= D233), C264 (= C273), R292 (= R301)
- binding phosphoric acid mono(formamide)ester: S50 (= S63), T51 (≠ L64), R52 (= R65), T53 (= T66), R101 (= R114), C264 (= C273), L265 (≠ M274), R292 (= R301)
- binding norvaline: L123 (= L136), N160 (= N172), D224 (= D233), S228 (= S237), M229 (= M238)
P9WIT9 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
48% identity, 79% coverage: 48:313/336 of query aligns to 34:304/307 of P9WIT9
- STRT 50:53 (≠ SLRT 63:66) binding carbamoyl phosphate
- Q77 (≠ G90) binding carbamoyl phosphate
- R101 (= R114) binding carbamoyl phosphate
- HPCQ 128:131 (= HPCQ 141:144) binding carbamoyl phosphate
- N160 (= N172) binding L-ornithine
- D224 (= D233) binding L-ornithine
- SM 228:229 (= SM 237:238) binding L-ornithine
- CL 264:265 (≠ CM 273:274) binding carbamoyl phosphate
- R292 (= R301) binding carbamoyl phosphate
7np0A Crystal structure of mycobacterium tuberculosis argf in complex with (4-nitrophenyl)boronic acid.
47% identity, 82% coverage: 39:313/336 of query aligns to 26:302/305 of 7np0A
7novA Crystal structure of mycobacterium tuberculosis argf in complex with (4-methyl-3-nitrophenyl)boronic acid.
48% identity, 79% coverage: 48:313/336 of query aligns to 35:299/302 of 7novA
- active site: R96 (= R114), H123 (= H141), Q126 (= Q144), D219 (= D233), C259 (= C273), R287 (= R301)
- binding (4-methyl-3-nitro-phenyl)-oxidanyl-oxidanylidene-boron: R53 (= R65), F56 (≠ V68), E77 (= E95), V85 (≠ L103), Y89 (= Y107), L260 (≠ M274), A284 (= A298), R287 (= R301)
7nnzB Crystal structure of mycobacterium tuberculosis argf in complex with 5-methyl-4-phenylthiazol-2-amine.
47% identity, 79% coverage: 48:313/336 of query aligns to 34:294/297 of 7nnzB
P00481 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Rattus norvegicus (Rat) (see 2 papers)
38% identity, 93% coverage: 3:314/336 of query aligns to 32:343/354 of P00481
- R92 (= R65) mutation to L: Strong decrease in ornithine carbamoyltransferase activity.
- C303 (= C273) mutation to S: Increases KM for ornithine 5-fold and decreases kcat 20-fold.
Sites not aligning to the query:
- 1:32 modified: transit peptide, Mitochondrion
P00480 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Homo sapiens (Human) (see 31 papers)
39% identity, 91% coverage: 9:313/336 of query aligns to 38:342/354 of P00480
- G39 (≠ K10) to C: in OTCD; late onset; dbSNP:rs72554306
- R40 (= R11) to H: in OTCD; late onset; dbSNP:rs72554308
- L43 (= L14) to F: in dbSNP:rs72554309
- K46 (≠ T17) to R: in dbSNP:rs1800321
- Y55 (≠ E26) to D: in OTCD; late onset; dbSNP:rs72554319
- L63 (≠ I34) to P: in OTCD; late onset; dbSNP:rs72554324
- K88 (= K61) modified: N6-acetyllysine; alternate; to N: in OTCD; late onset; dbSNP:rs72554339
- STRT 90:93 (≠ SLRT 63:66) binding carbamoyl phosphate
- G100 (= G73) to D: in OTCD; late onset; dbSNP:rs72554349
- F101 (≠ V74) to L: in dbSNP:rs1133135
- L111 (= L84) to P: in dbSNP:rs1800324
- T125 (≠ E98) to M: in OTCD; neonatal; dbSNP:rs72554356
- D126 (= D99) to G: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; 0.9% of wild-type activity; dbSNP:rs72554358
- R129 (= R102) to H: in OTCD; early onset; decreased ornithine carbamoyltransferase activity; 2.1% of wild-type activity; dbSNP:rs66656800
- A140 (= A113) to P: in OTCD; late onset; dbSNP:rs72556260
- R141 (= R114) binding carbamoyl phosphate; to Q: in OTCD; most common variant; loss of ornithine carbamoyltransferase activity; activity is 100-fold lower; dbSNP:rs68026851
- H168 (= H141) binding carbamoyl phosphate
- Q171 (= Q144) binding carbamoyl phosphate
- I172 (= I145) to M: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; dbSNP:rs72556280
- Y176 (≠ A149) to C: in OTCD; late onset; dbSNP:rs72556283
- TL 178:179 (= TL 151:152) natural variant: Missing (in OTCD; neonatal)
- Y183 (≠ G156) to D: in OTCD; late onset; dbSNP:rs72556292
- G188 (≠ D161) to R: in OTCD; neonatal; dbSNP:rs72556294
- G195 (= G168) to R: in OTCD; loss of ornithine carbamoyltransferase activity; dbSNP:rs67294955
- D196 (= D169) to V: in OTCD; neonatal; decreased ornithine carbamoyltransferase activity; 3.7% activity; dbSNP:rs72556300
- L201 (≠ A174) to P: in OTCD; neonatal; dbSNP:rs72558407
- S207 (≠ L180) to R: in OTCD; neonatal; dbSNP:rs72558415
- A209 (≠ G182) to V: in OTCD; neonatal; dbSNP:rs72558417
- M213 (≠ F186) to K: in OTCD; late onset
- H214 (= H187) to Y: in OTCD; neonatal; dbSNP:rs72558420
- P220 (= P193) to A: in OTCD; late onset; dbSNP:rs72558425
- P225 (= P198) to T: in OTCD; late onset; dbSNP:rs72558428
- L244 (≠ I214) to Q: in OTCD; late onset; dbSNP:rs72558436
- T262 (= T232) to K: in OTCD; mild; dbSNP:rs67333670
- T264 (≠ V234) to A: in OTCD; late onset; decreased ornithine carbamoyltransferase activity; 8.9% activity; dbSNP:rs72558444; to I: in OTCD; late onset; dbSNP:rs67156896
- W265 (= W235) to L: in OTCD; mild; dbSNP:rs72558446
- G269 (= G239) to E: in OTCD; neonatal; dbSNP:rs72558450
- Q270 (= Q240) to R: in dbSNP:rs1800328
- E272 (≠ D242) natural variant: Missing (in OTCD; late onset; dbSNP:rs72558452)
- R277 (= R247) to Q: in OTCD; late onset; dbSNP:rs66724222; to W: in OTCD; late onset; dbSNP:rs72558454
- H302 (= H272) to L: in OTCD; female; late onset; dbSNP:rs67993095; to Y: in OTCD; neonatal; dbSNP:rs72558463
- C303 (= C273) to R: in OTCD; neonatal; dbSNP:rs67468335
- CL 303:304 (≠ CM 273:274) binding carbamoyl phosphate
- E309 (≠ Q280) natural variant: Missing (in OTCD; late onset)
- R330 (= R301) binding carbamoyl phosphate
- T333 (≠ V304) natural variant: T -> A
- S340 (≠ K311) to P: in OTCD; late onset; dbSNP:rs72558489
Sites not aligning to the query:
- 1:32 modified: transit peptide, Mitochondrion
- 15 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-23 and G-26.
- 23 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-26.
- 26 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-23.
- 343 T → K: in OTCD; late onset; dbSNP:rs72558491
1othA Crystal structure of human ornithine transcarbamoylase complexed with n-phosphonacetyl-l-ornithine (see paper)
39% identity, 91% coverage: 9:314/336 of query aligns to 5:310/321 of 1othA
- active site: R59 (= R65), T60 (= T66), V87 (≠ E93), R108 (= R114), H135 (= H141), Q138 (= Q144), D230 (= D233), C270 (= C273), R297 (= R301)
- binding n-(phosphonoacetyl)-l-ornithine: S57 (= S63), T58 (≠ L64), R59 (= R65), T60 (= T66), R108 (= R114), L130 (= L136), H135 (= H141), N166 (= N172), D230 (= D233), S234 (= S237), M235 (= M238), C270 (= C273), L271 (≠ M274), R297 (= R301)
1c9yA Human ornithine transcarbamylase: crystallographic insights into substrate recognition and catalytic mechanism (see paper)
39% identity, 91% coverage: 9:314/336 of query aligns to 5:310/321 of 1c9yA
- active site: R59 (= R65), T60 (= T66), V87 (≠ E93), R108 (= R114), H135 (= H141), Q138 (= Q144), D230 (= D233), C270 (= C273), R297 (= R301)
- binding phosphoric acid mono(formamide)ester: S57 (= S63), T58 (≠ L64), R59 (= R65), T60 (= T66), R108 (= R114), C270 (= C273), L271 (≠ M274), R297 (= R301)
- binding norvaline: L130 (= L136), N166 (= N172), D230 (= D233), S234 (= S237), M235 (= M238)
Query Sequence
>WP_012502229.1 NCBI__GCF_000020505.1:WP_012502229.1
MSKQQTGTKKRDFLGFTGLDAEKIIELFDYSLHIKQQREANRQTAEFRPIRHKTVAMIFN
KPSLRTRVSFELGVYELGGHAISLEGKSIGVGERESVEDIARLLSRYNDAIVARLHEHEI
IETLAAHASIPVINALTDLSHPCQILADAFTLYEKGLWRDDIKVVFVGDGNNVANSWIEL
AGILPFHFVLTCPEGYLPDETLLKQARANAKSVIEIVHDPMEAAKDADVLYTDVWTSMGQ
EDEMAERLKTFEPYQINSKMMAEAKSSAVVMHCMPAHRGQEISAEVMDGPQSIIIDEAEN
RLHVQKALMVKLMNHDVYRKFHLTHRLNRAASQLKA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory