SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012502591.1 NCBI__GCF_000020505.1:WP_012502591.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
56% identity, 92% coverage: 6:257/273 of query aligns to 5:256/258 of 4h7oA

query
sites
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binding arginine: T51 (≠ S52), S53 (≠ H54), Y127 (≠ F128), Q131 (≠ R132)
binding cysteine: D88 (= D89), P89 (= P90), D153 (= D154), H154 (= H155), G180 (= G181), R188 (= R189), H189 (= H190)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
55% identity, 92% coverage: 6:257/273 of query aligns to 9:260/262 of 1t3dA

query
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binding cysteine: D92 (= D89), P93 (= P90), D157 (= D154), G184 (= G181), R192 (= R189), H193 (= H190)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
56% identity, 92% coverage: 6:255/273 of query aligns to 5:254/258 of 8i04A

query
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binding serine: D88 (= D89), P89 (= P90), D153 (= D154), H154 (= H155), G180 (= G181), R188 (= R189), H189 (= H190)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
54% identity, 93% coverage: 5:258/273 of query aligns to 4:257/257 of 1ssqD

query
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binding cysteine: D88 (= D89), D153 (= D154), H154 (= H155)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
54% identity, 93% coverage: 3:257/273 of query aligns to 9:263/272 of 3gvdI

query
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G

V

V

P

P

A

A

H

R

I

I

V

V

G

G

R

K

T

P

E

E

V

S

P

D

E

K

P

P

S

S

L

L

D

D

M

M

N

D

Q

Q

R

H

L

F

binding cysteine: D95 (= D89), P96 (= P90), D160 (= D154), G186 (= G180), G187 (= G181), R195 (= R189)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
56% identity, 87% coverage: 6:243/273 of query aligns to 8:245/246 of 8i09A

query
sites
8i09A

I

V

W

W

S

K

L

N

I

I

R

K

E

A

E

E

A

A

R

R

L

A

E

L

C

A

E

D

R

C

E

E

P

P

E

M

I

L

R

A

L

S

F

F

L

Y

E

H

Q

A

H

T

I

L

L

L

R

K

Y

H

D

E

E

N

F

L

A

G

P

S

A

A

L

L

A

S

M

Y

L

M

L

L

S

A

V

N

K

K

L

L

G

A

S

S

K

P

H

I

F

M

P

P

P

A

P

I

V

A

L

I

E

R

G

E

I

V

F

V

E

E

D

E

F

A

Y

Y

R

A

Q

A

N

D

P

P

E

E

S

M

V

I

R

A

C

S

A

A

A

A

C

C

D

D

M

I

V

Q

A

A

T

V

Q

R

E

T

R

R

D
|
D

P

P

A

A

A

V

V

D

N

K

Y

Y

F

S

E

T

I

P

M

L

L

L

F

Y

L

L

K

K

G

G

Y

F

Q

H

A

A

L

L

Q

Q

S

A

Y

Y

R

R

L

I

A

G

H

H

W

W

L

L

W

W

Q

N

N

K

G

G

R

R

K

R

S

A

L

L

A

A

Y

I

F

F

L

L

Q

Q

N

N

R

Q

M

V

S

S

E

V

V

S

F

F

A

Q

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

K

I

I

G

G

K

R

G

G

I

I

L

M

L

L

D
|
D

H
|
H

A

A

T

T

S

G

L

I

V

V

I

V

G
|
G

E

E

T

T

A

A

V

V

V

I

E

E

D

D

N

D

V

V

S

S

I

I

L

L

H

Q

E

S

V

V

T

T

L

L

G

G

G
|
G

T
|
T

G

G

K

K

E

T

G

S

G

G

D

D

R
|
R

H
|
H

P

P

K

K

V

I

G

R

K

E

S

G

V

V

M

M

I

I

G
|
G

A

A

G

G

A

A

K

K

I

I

L

L

G

G

N

N

I

I

K

E

I

V

G

G

E

R

G

G

A

A

K
|
K

I
|
I

G
|
G

A

A

G

G

S

S

V

V

V

V

L
|
L

D

Q

D

P

V

V

P

P

P

P

H

H

Y

T

T

T

V

A

A
|
A

G

G

V

V

P

P

A

A

H

R

I

I

V

V

G

G

R

K

binding cysteine: D91 (= D89), D156 (= D154), H157 (= H155), G164 (= G162), R191 (= R189), H192 (= H190)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (= G181), T184 (= T182), G202 (= G200), K218 (= K216), I219 (= I217), G220 (= G218), L226 (= L224), A236 (= A234)

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
56% identity, 86% coverage: 6:241/273 of query aligns to 9:244/244 of 8i06A

query
sites
8i06A

I

V

W

W

S

K

L

N

I

I

R

K

E

A

E

E

A

A

R

R

L

A

E

L

C

A

E

D

R

C

E

E

P

P

E

M

I

L

R

A

L

S

F

F

L

Y

E

H

Q

A

H

T

I

L

L

L

R

K

Y

H

D

E

E

N

F

L

A

G

P

S

A

A

L

L

A

S

M

Y

L

M

L

L

S

A

V

N

K

K

L

L

G

A

S

S

K

P

H

I

F

M

P

P

P

A

P

I

V

A

L

I

E

R

G

E

I

V

F

V

E

E

D

E

F

A

Y

Y

R

A

Q

A

N

D

P

P

E

E

S

M

V

I

R

A

C

S

A

A

A

A

C

C

D

D

M

I

V

Q

A

A

T

V

Q

R

E

T

R

R

D

D

P
|
P

A

A

A

V

V

D

N

K

Y

Y

F

S

E

T

I

P

M

L

L

L

F

Y

L

L

K

K

G

G

Y

F

Q

H

A

A

L

L

Q

Q

S

A

Y

Y

R

R

L

I

A

G

H

H

W

W

L

L

W

W

Q

N

N

K

G

G

R

R

K

R

S

A

L

L

A

A

Y

I

F

F

L

L

Q

Q

N

N

R

Q

M

V

S

S

E

V

V

S

F

F

A

Q

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

K

I

I

G

G

K

R

G

G

I

I

L

M

L

L

D

D

H
|
H

A

A

T

T

S

G

L

I

V

V

I

V

G

G

E

E

T

T

A

A

V

V

V

I

E

E

D

D

N

D

V

V

S

S

I

I

L
|
L

H
x
Q

E

S

V

V

T

T

L

L

G

G

G

G

T

T

G

G

K

K

E

T

G

S

G

G

D

D

R

R

H

H

P

P

K

K

V

I

G

R

K

E

S

G

V

V

M

M

I

I

G
|
G

A
|
A

G

G

A

A

K

K

I

I

L

L

G

G

N

N

I

I

K

E

I

V

G

G

E

R

G

G

A

A

K

K

I

I

G
|
G

A
|
A

G

G

S

S

V

V

V

V

L

L

D

Q

D

P

V

V

P

P

P

P

H

H

Y

T

T
|
T

V

A

A
|
A

G

G

V

V

P

P

A

A

H

R

I

I

V

V

binding coenzyme a: H158 (= H155), L177 (= L174), Q178 (≠ H175), G203 (= G200), A204 (= A201), G221 (= G218), A222 (= A219), T235 (= T232), A237 (= A234)
binding cysteine: P93 (= P90), H158 (= H155)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
54% identity, 93% coverage: 5:257/273 of query aligns to 9:260/261 of 6wyeA

query
sites
6wyeA

D

D

I

L

W

W

S

H

L

T

I

I

R

R

E

E

E

E

A

T

R

A

L

A

E

A

C

A

E

A

R

A

E

E

P

P

E

M

I

L

R

A

L

S

F

F

L

L

E

H

Q

Q

H

T

I

V

L

L

R

R

Y

H

D

E

E

S

F

L

A

G

P

S

A

V

L

L

A

A

M

Y

L

H

L

L

S

S

V

S

K

K

L

L

G

G

S

S

K

P

H

I

F

M

P

D

P

V

P

R

V

A

L

L

E

F

G

E

I

I

F

Y

E

Q

D

Q

F

A

Y

L

R

G

Q

S

N

D

P

T

E

Q

S

I

V

S

R

K

C

C

A

V

A

E

C

A

D

D

M

L

V

K

A

A

T

I

Q

Y

E

E

R

R

D
|
D

P
|
P

A

A

A

C

V

D

N

E

Y

Y

F

S

E

L

I

P

M

L

L

L

F

Y

L

F

K

K

G

G

Y

F

Q

H

A

A

L

I

Q

Q

S

A

Y

H

R

R

L

I

A

N

H

H

W

R

L

L

W

Y

Q

L

N

D

G

G

R

R

K

K

S

T

L

L

A

A

Y

Y

F

F

L

L

Q

Q

N

N

R

R

M

M

S

S

E

E

V

V

F

F

A

G

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

R

I

L

G

G

K

Y

G

G

I

L

L

M

L

L

D
|
D

H
|
H

A

A

T

T

S

G

L

F

V

V

I

A

G

G

E

E

T

T

A

A

V

V

V

L

E

G

D

N

N

N

V

I

S

S

I

I

L

L

H

H

E

G

V

V

T

T

L

L

G

G

G
|
G

T

S

G

G

K

K

E

E

G

G

G

G

D

D

R
|
R

H
|
H

P

P

K

K

V

I

G

G

K

D

S

G

V

V

M

M

I

I

G

G

A

A

G

N

A

A

K

S

I

I

L

L

G

G

N

N

I

I

K

R

I

I

G

G

E

S

G

N

A

A

K

K

I

I

G

G

A

A

G

G

S

S

V

V

V

V

L

V

D

S

D

D

V

V

P

P

P

P

H

S

Y

I

T

T

V

V

A

V

G

G

V

V

P

P

A

A

H

K

I

P

V

V

G

A

R

R

T

S

E

-

V

L

P

K

E

T

P

P

S

S

L

A

D

D

M

M

N

D

Q

Q

R

N

L

I

binding (2S)-2-hydroxybutanedioic acid: D93 (= D89), P94 (= P90), D158 (= D154), H159 (= H155), G185 (= G181), R193 (= R189), H194 (= H190)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
55% identity, 86% coverage: 5:238/273 of query aligns to 7:240/243 of 7ra4A

query
sites
7ra4A

D

D

I

L

W

W

S

H

L

T

I

I

R

R

E

E

E

E

A

T

R

A

L

A

E

A

C

A

E

A

R

A

E

E

P

P

E

M

I

L

R

A

L

S

F

F

L

L

E

H

Q

Q

H

T

I

V

L

L

R

R

Y

H

D

E

E

S

F

L

A

G

P

S

A

V

L

L

A

A

M

Y

L

H

L

L

S

S

V

S

K

K

L

L

G

G

S

S

K

P

H

I

F

M

P

D

P

V

P

R

V

A

L

L

E

F

G

E

I

I

F

Y

E

Q

D

Q

F

A

Y

L

R

G

Q

S

N

D

P

T

E

Q

S

I

V

S

R

K

C

C

A

V

A

E

C

A

D

D

M

L

V

K

A

A

T

I

Q

Y

E

E

R

R

D
|
D

P
|
P

A

A

A

C

V

D

N

E

Y

Y

F

S

E

L

I

P

M

L

L

L

F

Y

L

F

K

K

G

G

Y

F

Q

H

A

A

L

I

Q

Q

S

A

Y

H

R

R

L

I

A

N

H

H

W

R

L

L

W

Y

Q

L

N

D

G

G

R

R

K

K

S

T

L

L

A

A

Y

Y

F

F

L

L

Q

Q

N

N

R

R

M

M

S

S

E

E

V

V

F

F

A

G

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

R

I

L

G

G

K

Y

G

G

I

L

L

M

L

L

D
|
D

H
|
H

A

A

T

T

S

G

L

F

V

V

I

A

G

G

E

E

T

T

A

A

V

V

V

L

E

G

D

N

N

N

V

I

S

S

I

I

L

L

H

H

E

G

V

V

T

T

L

L

G

G

G

G

T

S

G

G

K

K

E

E

G

G

G

G

D

D

R
|
R

H
|
H

P

P

K

K

V

I

G

G

K

D

S

G

V

V

M

M

I

I

G

G

A

A

G

N

A

A

K

S

I

I

L

L

G

G

N

N

I

I

K

R

I

I

G

G

E

S

G

N

A

A

K

K

I

I

G

G

A

A

G

G

S

S

V

V

V

V

L

V

D

S

D

D

V

V

P

P

P

P

H

S

Y

I

T

T

V

V

A

V

G

G

V

V

P

P

A

A

binding serine: D91 (= D89), P92 (= P90), D156 (= D154), H157 (= H155), R191 (= R189), H192 (= H190)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
53% identity, 86% coverage: 6:241/273 of query aligns to 8:243/243 of 4n69A

query
sites
4n69A

I

V

W

W

S

G

L

Q

I

I

R

K

E

A

E

E

A

A

R

R

L

R

E

D

C

A

E

E

R

S

E

E

P

P

E

A

I

L

R

A

L

S

F

Y

L

L

E

Y

Q

S

H

T

I

I

L

L

R

S

Y

H

D

S

E

S

F

L

A

E

P

R

A

S

L

L

A

S

M

F

L

H

L

L

S

G

V

N

K

K

L

L

G

C

S

S

K

S

H

T

F

L

P

L

P

S

P

T

V

L

L

L

E

Y

G

D

I

L

F

F

E

L

D

N

F

A

Y

F

R

S

Q

S

N

D

P

P

E

S

S

L

V

R

R

S

C

A

A

A

A

V

C

A

D

D

M

L

V

R

A

A

T

A

Q

R

E

E

R

R

D

D

P

P

A

A

A

C

V

V

N

S

Y

Y

F

S

E

H

I

C

M

L

L

L

F

N

L

Y

K

K

G

G

Y

F

Q

L

A

A

L

C

Q

Q

S

A

Y

H

R

R

L

V

A

A

H

H

W

L

L

L

W

W

Q

R

N

Q

G

S

R

R

K

R

S

P

L

L

A

A

Y

L

F

A

L

L

Q

H

N

S

R

R

M

I

S

A

E

N

V

V

F

F

A

A

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

R

I

I

G

G

K

K

G

G

I

I

L

L

L

F

D

D

H

H

A

A

T

T

S

G

L

V

V

V

I

V

G

G

E

E

T

T

A

A

V

V

V

I

E

G

D

N

N

N

V

V

S

S

I

I

L

L

H

H

E

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

V

G

G

G

G

D

D

R
|
R

H
|
H

P

P

K

K

V

I

G

G

K

D

S

G

V

V

M

L

I

I

G

G

A

A

G

G

A

A

K

T

I

I

L

L

G

G

N

N

I

I

K

K

I

I

G

G

E

E

G

G

A

A

K

K

I

V

G

G

A

A

G

G

S

S

V

V

V

V

L

L

D

I

D

D

V

V

P

P

P

P

H

R

Y

T

T

T

V

A

A

V

G

G

V

N

P

P

A

A

H

R

I

L

V

V

binding serine: G183 (= G181), R191 (= R189), H192 (= H190)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
51% identity, 89% coverage: 3:244/273 of query aligns to 6:247/250 of 4hzdA

query
sites
4hzdA

V

V

Q

D

D

P

I

I

W

W

S

H

L

S

I

I

R

R

E

A

E

E

A

A

R

E

L

E

E

A

C

T

E

R

R

N

E

D

P

P

E

V

I

L

R

G

L

A

F

F

L

L

E

Y

Q

A

H

T

I

I

L

L

R

N

Y

Q

D

P

E

S

F

L

A

E

P

E

A

A

L

V

A

M

M

H

L

R

L

I

S

A

V

E

K

R

L

L

G

G

S

H

K

P

H

D

F

V

P

S

P

A

P

D

V

I

L

L

E

R

G

Q

I

T

F

F

E

D

D

T

F

M

Y

L

R

E

Q

A

N

N

P

P

E

E

S

W

V

S

R

H

C

V

A

L

A

R

C

V

D

D

M

I

V

Q

A

A

T

V

Q

Y

E

D

R

R

D

D

P

P

A

A

A

Y

V

S

N

R

Y

F

F

M

E

D

I

P

M

V

L

L

F

Y

L

L

K

K

G

G

Y

F

Q

H

A

A

L

I

Q

Q

S

T

Y

H

R

R

L

L

A

A

H

H

W

W

L

L

W

Y

Q

K

N

Q

G

G

R

R

K

K

S

D

L

F

A

A

Y

Y

F

Y

L

L

Q

Q

N

S

R

R

M

S

S

S

E

S

V

I

F

F

A

Q

V

T

D

D

I

I

H

H

P

P

A

A

A

A

K

R

I

L

G

G

K

S

G

G

I

L

L

F

L

L

D

D

H
|
H

A

A

T

T

S

G

L

L

V

V

I

V

G

G

E

E

T

T

A

A

V

V

V

V

E

E

D

D

N

N

V

V

S

S

I

I

L

L

H

H

E

G

V

V

T

T

L

L

G

G

G

G

T

T

G

G

K

K

E

S

G

S

G

G

D

D

R

R

H

H

P

P

K

K

V

I

G

R

K

Q

S

G

V

V

M

L

I

I

G
|
G

A
|
A

G

G

A

A

K

K

I

I

L

L

G

G

N

N

I

I

K

Q

I

V

G

G

E

Q

G

C

A

S

K

K

I

I

G
x
A

A
|
A

G

G

S
|
S

V

V

V

V

L

L

D

K

D

S

V

V

P

P

P

H

H

N

Y

V

T
|
T

V

V

A
|
A

G
|
G

V

V

P

P

A

A

H

R

I

I

V
x
I

G

G

R

E

T

T

binding coenzyme a: H158 (= H155), G203 (= G200), A204 (= A201), A221 (≠ G218), A222 (= A219), T235 (= T232), A237 (= A234), I244 (≠ V241)
binding magnesium ion: S224 (= S221), A237 (= A234), G238 (= G235)

1sstA Serine acetyltransferase- complex with coa (see paper)
53% identity, 87% coverage: 5:241/273 of query aligns to 4:233/233 of 1sstA

query
sites
1sstA

D

D

I

V

W

W

S

Q

L

H

I

I

R

R

E

Q

E

E

A

A

R

K

L

E

E

L

C

A

E

E

R

N

E

E

P

P

E

M

I

L

R

A

L

S

F

F

L

F

E

H

Q

S

H

T

I

I

L

L

R

K

Y

H

D

Q

E

N

F

L

A

G

P

G

A

A

L

L

A

S

M

Y

L

L

L

L

S

A

V

N

K

K

L

L

G

A

S

N

K

P

H

I

F

M

P

P

P

A

P

I

V

S

L

L

E

R

G

E

I

I

F

I

E

E

D

E

F

A

Y

Y

R

Q

Q

S

N

N

P

P

E

S

S

I

V

I

R

D

C

C

A

A

A

A

C

C

D

D

M

I

V

Q

A

A

T

V

Q

R

E

H

R

R

D

D

P

P

A

A

A

V

V

E

N

L

Y

W

F

S

E

T

I

P

M

L

L

L

F

Y

L

L

K

K

G

G

Y

F

Q

H

A

A

L

I

Q

Q

S

S

Y

Y

R

R

L

I

A

T

H

H

W

Y

L

L

W

W

Q

N

N

Q

G

N

R

R

K

K

S

S

L

L

A

A

Y

L

F

Y

L

L

Q

Q

N

N

R

Q

M

I

S

S

E

V

V

A

F

F

A

D

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

K

I

I

G

G

K

H

G

G

I

I

L

M

L

F

D

D

H
|
H

A

A

T

T

S

G

L

I

V

V

I

V

G

G

E

E

T

T

A

S

V

V

V

I

E

E

D

N

N

D

V

V

S

S

I

I

L
|
L

H

Q

E

G

V

V

T

T

L

L

G

G

G
|
G

T
|
T

G

-

K

-

E

-

G

-

G

-

D

-

R

-

H

H

P

P

K

K

V

V

G

R

K

E

S

G

V

V

M

M

I

I

G

G

A
|
A

G

G

A

A

K

K

I

I

L
|
L

G

G

N

N

I

I

K

E

I

V

G

G

E

K

G

Y

A

A

K
|
K

I

I

G
|
G

A
|
A

G

N

S

S

V
|
V

V

V

L
|
L

D

N

D

P

V

V

P

P

P

E

H

Y

Y

A

T
|
T

V

A

A
|
A

G

G

V
|
V

P
|
P

A

A

H

R

I

I

V
|
V

binding coenzyme a: H154 (= H155), L173 (= L174), G180 (= G181), T181 (= T182), A193 (= A201), L198 (= L206), K208 (= K216), G210 (= G218), A211 (= A219), V214 (= V222), L216 (= L224), T224 (= T232), A226 (= A234), V228 (= V236), P229 (= P237), V233 (= V241)

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
52% identity, 86% coverage: 6:241/273 of query aligns to 4:233/233 of 4n6bA

query
sites
4n6bA

I

V

W

W

S

G

L

Q

I

I

R

K

E

A

E

E

A

A

R

R

L

R

E

D

C

A

E

E

R

S

E

E

P

P

E

A

I

L

R

A

L

S

F

Y

L

L

E

Y

Q

S

H

T

I

I

L

L

R

S

Y

H

D

S

E

S

F

L

A

E

P

R

A

S

L

L

A

S

M

F

L

H

L

L

S

G

V

N

K

K

L

L

G

C

S

S

K

S

H

T

F

L

P

L

P

S

P

T

V

L

L

L

E

Y

G

D

I

L

F

F

E

L

D

N

F

A

Y

F

R

S

Q

S

N

D

P

P

E

S

S

L

V

R

R

S

C

A

A

A

A

V

C

A

D

D

M

L

V

R

A

A

T

A

Q

R

E

E

R

R

D

D

P

-

A

-

A

-

V

V

N

S

Y

Y

F

S

E

H

I

C

M

L

L

L

F

N

L

Y

K

K

G

G

Y

F

Q

L

A

A

L

C

Q

Q

S

A

Y

H

R

R

L

V

A

A

H

H

W

L

L

L

W

W

Q

R

N

Q

G

S

R

R

K

R

S

P

L

L

A

A

Y

L

F

A

L

L

Q

H

N

S

R

R

M

I

S

A

E

N

V

V

F

F

A

A

V

V

D

D

I

I

H

H

P

P

A

A

A

A

K

R

I

I

G

G

K

K

G

G

I

I

L

L

L

F

D

D

H
|
H

A

A

T

T

S

G

L

V

V

V

I

V

G

G

E

E

T

T

A

A

V

V

V

I

E

G

D

N

N

N

V

V

S

S

I

I

L
|
L

H

H

E

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

-

K

-

E

-

G

G

G

G

D

D

R

R

H

H

P

P

K

K

V

I

G

G

K

D

S

G

V

V

M

L

I

I

G

G

A

A

G

G

A

A

K

T

I

I

L

L

G

G

N

N

I

I

K

K

I

I

G

G

E

E

G

G

A

A

K
|
K

I

V

G
|
G

A
|
A

G

G

S

S

V

V

V

V

L
|
L

D

I

D

D

V

V

P

P

P

P

H

R

Y

T

T

T

V

A

A
x
V

G

G

V

N

P

P

A

A

H

R

I

L

V

V

binding coenzyme a: H150 (= H155), L169 (= L174), G176 (= G181), K208 (= K216), G210 (= G218), A211 (= A219), L216 (= L224), V226 (≠ A234)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
39% identity, 54% coverage: 81:227/273 of query aligns to 106:252/280 of 7bw9A

query
sites
7bw9A

D

D

M

I

V

I

A

A

T

A

Q

Y

E

T

R

G

D
|
D

P
|
P

A

A

A

A

V

S

N

S

Y

L

F

A

E

M

I

I

M

I

L

R

F

S

L

Y

K

P

G

G

Y

I

Q

H

A

V

L

M

Q

M

S

I

Y

Q

R

R

L

V

A

A

H

H

W

I

L

L

W

Y

Q

M

N

N

G

G

R

D

K

I

S

E

L

Y

A

S

Y

R

F

E

L

L

Q

M

N

E

R

N

M

I

S

H

E

S

V

V

F

T

A

G

V

I

D

D

I

I

H

H

P

P

A

G

A

T

K

S

I

I

G

G

K

N

G

H

I

F

L

F

L

I

D
|
D

H
|
H

A

G

T

V

S

G

L

V

V

V

I

I

G

G

E

E

T

T

A

A

V

V

V

I

E

G

D

N

N

W

V

C

S

R

I

V

L

Y

H

Q

E

S

V

V

T

T

L

L

G

G

G
x
A

T

M

G

S

K

F

E

K

G

G

G

N

D

K

R
|
R

H
|
H

P

P

K

T

V

I

G

G

K

D

S

F

V

V

M

V

I

I

G

G

A

A

G

G

A

A

K

K

I

V

L

L

G

G

N

N

I

I

K

T

I

I

G

G

E

S

G

N

A

V

K

K

I

I

G

G

A

A

G

N

S

C

V

W

V

I

L

T

D

Q

D

N

V

I

binding cysteine: D114 (= D89), P115 (= P90), D179 (= D154), H180 (= H155), A206 (≠ G181), R214 (= R189), H215 (= H190)

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
37% identity, 59% coverage: 72:233/273 of query aligns to 99:268/270 of 3p47A

query
sites
3p47A

P

P

E

S

S

I

V

K

R

R

C

T

A

L

A

K

C

T

D

D

M

L

V

I

A

A

T

A

Q

Y

E

A

R

G

D

D

P

P

A

A

A

A

V

P

N

G

Y

L

F

S

E

L

I

I

M

I

L

R

F

C

L

Y

K

P

G

G

Y

F

Q

Q

A

A

L

V

Q

I

S

V

Y

Y

R

R

L

I

A

A

H

H

W

V

L

L

W

Y

Q

E

N

C

G

G

R

E

K

R

S

Y

L

Y

A

C

Y

R

F

E

L

M

Q

M

N

E

R

S

M

V

S

H

E

S

V

Y

F

T

A

S

V

I

D

D

I

I

H

H

P

P

A

G

A

A

K

S

I

I

G

K

K

G

G

H

I

F

L

F

L

I

D

D

H

H

A

G

T

V

S

G

L

V

V

V

I

I

G

G

E

E

T

T

A

A

V

I

V

I

E

G

D

E

N

W

V

C

S

R

I

I

L

Y

H

Q

E

S

V

V

T

T

L

L

-

G

-
x
A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

G

G

G

T

D

K

R
|
R

H
|
H

P

P

K

T

V

V

G

G

K

D

S

Y

V

V

M

T

I

I

G

G

A

T

G

G

A

A

K

K

I

V

L

L

G

G

N

N

I

I

K

I

I

V

G

G

E

S

G

H

A

V

K

R

I

I

G

G

A

A

G

N

S

C

V

W

V

I

L

D

D

R

D

D

V

V

P

D

P

S

H

N

Y

Q

T

T

V

V

binding cysteine: A208 (vs. gap), H210 (vs. gap), R224 (= R189), H225 (= H190)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
37% identity, 59% coverage: 72:233/273 of query aligns to 97:266/267 of 3q1xA

query
sites
3q1xA

P

P

E

S

S

I

V

K

R

R

C

T

A

L

A

K

C

T

D

D

M

L

V

I

A

A

T

A

Q

Y

E

A

R

G

D

D

P

P

A

A

A

A

V

P

N

G

Y

L

F

S

E

L

I

I

M

I

L

R

F

C

L

Y

K

P

G

G

Y

F

Q

Q

A

A

L

V

Q

I

S

V

Y

Y

R

R

L

I

A

A

H

H

W

V

L

L

W

Y

Q

E

N

C

G

G

R

E

K

R

S

Y

L

Y

A

C

Y

R

F

E

L

M

Q

M

N

E

R

S

M

V

S

H

E

S

V

Y

F

T

A

S

V

I

D

D

I

I

H

H

P

P

A

G

A

A

K

S

I

I

G

K

K

G

G

H

I

F

L

F

L

I

D

D

H

H

A

G

T

V

S

G

L

V

V

V

I

I

G

G

E

E

T

T

A

A

V

I

V

I

E

G

D

E

N

W

V

C

S

R

I

I

L

Y

H

Q

E

S

V

V

T

T

L

L

-

G

-

A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

G

G

G

T

D

K

R
|
R

H
|
H

P

P

K

T

V

V

G

G

K

D

S

Y

V

V

M

T

I

I

G

G

A

T

G

G

A

A

K

K

I

V

L

L

G

G

N

N

I

I

K

I

I

V

G

G

E

S

G

H

A

V

K

R

I

I

G

G

A

A

G

N

S

C

V

W

V

I

L

D

D

R

D

D

V

V

P

D

P

S

H

N

Y

Q

T

T

V

V

binding serine: H208 (vs. gap), R222 (= R189), H223 (= H190)

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
31% identity, 38% coverage: 137:241/273 of query aligns to 71:181/200 of 1krrA

query
sites
1krrA

F

Y

A

G

V

S

D

N

I

I

H

H

P

-

A

-

A

-

K

-

I

I

G

G

K

R

G

N

I

F

L

Y

L

A

D
x
N

H

F

A

N

T

L

S

T

L

I

V

V

I

D

G

D

E

Y

T

T

A

V

V

T

V

I

E

G

D

D

N

N

V

V

S

L

I
|
I

L
x
A

H
x
P

E

N

V

V

T

T

L

L

G

S

G

V

T
|
T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

G

N

G

G

D

E

R

M

H

Y

P

S

-

F

-

P

-

I

K

T

V

I

G

G

K

N

S

N

V

V

M

W

I

I

G
|
G

A
x
S

G

H

A

V

K

V

I

I

L
x
N

G

P

N

G

I

V

K

T

I

I

G

G

E

D

G

N

A

S

K

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

L

T

D

K

D

D

V

I

P

P

P

P

H

N

Y

V

T

V

V

A

A
|
A

G

G

V

V

P
|
P

A

C

H

R

I

V

V

I

binding acetyl coenzyme *a: N84 (≠ D154), I103 (= I173), A104 (≠ L174), P105 (≠ H175), T112 (= T182), H114 (vs. gap), G140 (= G200), S141 (≠ A201), N146 (≠ L206), G158 (= G218), A159 (= A219), A174 (= A234), P177 (= P237)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
31% identity, 38% coverage: 137:241/273 of query aligns to 71:181/201 of 1krvA

query
sites
1krvA

F

Y

A

G

V

S

D

N

I

I

H

H

P

-

A

-

A

-

K

-

I

I

G

G

K

R

G

N

I

F

L
x
Y

L

A

D
x
N

H

F

A

N

T

L

S

T

L

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

V

T

V

I

E

G

D

D

N

N

V

V

S

L

I

I

L
x
A

H

P

E

N

V

V

T

T

L

L

G
x
S

G

V

T
|
T

G

G

-

H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

G

N

G

G

D

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

K

T

V

I

G

G

K

N

S

N

V

V

M
x
W

I

I

G
|
G

A
x
S

G

H

A

V

K

V

I

I

L
x
N

G

P

N

G

I

V

K

T

I

I

G

G

E

D

G

N

A

S

K

V

I

I

G

G

A
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A

G

G

S

S

V

I

V

V

L

T

D

K

D

D

V

I

P

P

P

P

H

N

Y

V

T

V

V

A

A

A

G

G

V

V

P
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P

A

C

H
x
R

I

V

V

I

binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (≠ L152), N84 (≠ D154), V90 (= V160), D92 (≠ G162), M126 (≠ R189)
binding coenzyme a: A104 (≠ L174), S110 (≠ G180), T112 (= T182), W138 (≠ M198), G140 (= G200), S141 (≠ A201), N146 (≠ L206), A159 (= A219), P177 (= P237), R179 (≠ H239)

Sites not aligning to the query:

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
31% identity, 38% coverage: 137:241/273 of query aligns to 71:181/201 of 1kruA

query
sites
1kruA

F

Y

A

G

V

S

D

N

I

I

H

H

P

-

A

-

A

-

K

-

I

I

G

G

K

R

G

N

I

F

L
x
Y

L

A

D
x
N

H

F

A

N

T

L

S

T

L

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

V

T

V

I

E

G

D

D

N

N

V

V

S
x
L

I

I

L

A

H

P

E

N

V

V

T

T

L

L

G

S

G

V

T

T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

G

N

G

G

D

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

K

T

V

I

G

G

K

N

S

N

V

V

M
x
W

I

I

G
|
G

A
x
S

G

H

A

V

K

V

I

I

L

N

G

P

N

G

I

V

K

T

I

I

G

G

E

D

G

N

A

S

K

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

L

T

D

K

D

D

V

I

P

P

P

P

H

N

Y

V

T

V

V

A

A

A

G

G

V

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P
|
P

A

C

H

R

I

V

V

I

binding coenzyme a: H114 (vs. gap), W138 (≠ M198), G140 (= G200), S141 (≠ A201), G158 (= G218), A159 (= A219), P177 (= P237)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: Y82 (≠ L152), Y82 (≠ L152), N84 (≠ D154), V90 (= V160), D92 (≠ G162), L102 (≠ S172), H114 (vs. gap), M126 (≠ R189)

Sites not aligning to the query:

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 38% coverage: 137:241/273 of query aligns to 72:182/203 of P07464

query
sites
P07464

F

Y

A

G

V

S

D

N

I

I

H

H

P

-

A

-

A

-

K

-

I

I

G

G

K

R

G

N

I

F

L

Y

L

A

D
x
N

H

F

A

N

T

L

S

T

L

I

V

V

I

D

G
x
D

E

Y

T

T

A

V

V

T

V

I

E

G

D

D

N

N

V

V

S

L

I

I

L

A

H

P

E

N

V

V

T

T

L

L

G

S

G

V

T

T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

G

N

G

G

D

E

R

M

H

Y

P

S

-

F

-

P

-

I

K

T

V

I

G

G

K

N

S

N

V

V

M

W

I

I

G

G

A
x
S

G

H

A

V

K

V

I

I

L

N

G

P

N

G

I

V

K

T

I

I

G

G

E

D

G

N

A

S

K

V

I

I

G

G

A
|
A

G

G

S

S

V

I

V

V

L
x
T

D
x
K

D

D

V

I

P

P

P

P

H

N

Y

V

T

V

V

A

A

A

G

G

V

V

P

P

A

C

H
x
R

I

V

V

I

N85 (≠ D154) binding in other chain; binding in other chain
D93 (≠ G162) binding substrate
H115 (vs. gap) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A201) binding in other chain
A160 (= A219) binding in other chain
TK 165:166 (≠ LD 224:225) binding acetyl-CoA
R180 (≠ H239) binding acetyl-CoA

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
71 binding substrate
183 binding in other chain

Query Sequence

>WP_012502591.1 NCBI__GCF_000020505.1:WP_012502591.1
MSVQDIWSLIREEARLECEREPEIRLFLEQHILRYDEFAPALAMLLSVKLGSKHFPPPVL
EGIFEDFYRQNPESVRCAACDMVATQERDPAAVNYFEIMLFLKGYQALQSYRLAHWLWQN
GRKSLAYFLQNRMSEVFAVDIHPAAKIGKGILLDHATSLVIGETAVVEDNVSILHEVTLG
GTGKEGGDRHPKVGKSVMIGAGAKILGNIKIGEGAKIGAGSVVLDDVPPHYTVAGVPAHI
VGRTEVPEPSLDMNQRLVFPEKHKQTGTHHSCL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory